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molecular variability of pseudallescheria boydii, a neurotropic opportunist.the sequences of the internal transcribed spacer (its) ribosomal dna (rdna) domain data obtained by restriction fragment length polymorphism analysis with 18s rdna and fingerprinting (m13) for clinical and environmental strains of pseudallescheria boydii (anamorph, scedosporium apiospermum) were compared to those for related species of pseudallescheria, petriella, and scedosporium. the infraspecific variability of p. boydii was considerable. there were five different lengths in the 18s rdnas wit ...200010970369
molecular phylogeny of the pseudallescheria boydii species complex: proposal of two new species.pseudallescheria boydii (anamorph scedosporium apiospermum) is the species responsible for human scedosporiosis, a fungal infection with a high mortality rate and which is difficult to treat. recently, it has been demonstrated that high genetic variation exists within this species. we have performed a morphological and molecular study involving numerous strains of clinical or environmental origins and from different countries. the analysis of partial sequences of the beta-tubulin (two loci) and ...200516207945
intracavitary voriconazole for the treatment of hemoptysis complicating pseudallescheria angusta pulmonary mycetomas in fibrocystic sarcoidosis.pulmonary mycetomas often occur in fibrocystic sarcoidosis. when this condition is complicated by hemoptysis, definitive surgery is usually precluded because of poor lung function. intracavitary antifungal therapy has been described for the treatment of symptomatic pulmonary mycetomas. we report the first use of intracavitary voriconazole in the management of a pseudallescheria angusta pulmonary mycetoma complicated by hemoptysis in a patient with fibrocystic sarcoidosis and renal transplant.201120831365
structural analysis of glucosylceramides (glccer) from species of the pseudallescheria/scedosporium complex.glucosylceramides (glccer) are the main neutral glycosphingolipids expressed in fungal cells. in this work, glucosylceramides (glccer) were extracted from three strains of scedosporium (pseudallescheria) boydii, one strain of pseudallescheria ellipsoidea and one strain of pseudallescheria angusta and purified by several chromatographic steps. using high-performance thin layer chromatography (hptlc), we found a similarity between glccer obtained from all of the analysed strains. a detailed struct ...201626781373
intra- and interspecific diversity of ultrastructural markers in scedosporium.ultrastructural features of conidia, lateral walls of aerial and submerged hyphal cells, and of septal pore apparatus of scedosporium apiospermum, s. boydii, pseudallescheria angusta and scedosporium aurantiacum were studied. submerged hyphal cells possessed a thick extracellular matrix. crystalline satellites accessory to the septal pore apparatus were revealed. fundamental ultrastructural features appeared to be heterogeneous at low taxonomic levels. the closely interrelated members of the s. ...201626781370
the 'species complex' issue in clinically relevant fungi: a case study in scedosporium apiospermum.the genus scedosporium currently comprises six species, scedosporium apiospermum, scedosporium boydii, pseudallescheria angusta, scedosporium minutisporum, scedosporium dehoogii, and scedosporium aurantiacum, most of which can be distinguished with the primary fungal dna barcode, the its1/2 region of the rdna gene cluster. in the present study, four additional genetic loci were explored from a phylogenetic point of view enabling a barcoding approach based on k2p pairwise distances to resolve the ...201626781369
susceptibility and diversity in the therapy-refractory genus scedosporium.scedosporium species show decreased susceptibility to the majority of systemic antifungal drugs. acquired resistance is likely to disseminate differentially with the mode of exchange of genetic material between lineages. inter- and intraspecific diversities of scedosporium species were analyzed for three partitions (rdna internal transcribed spacer gene [its], partial β-tubulin gene, and amplified fragment length polymorphism profiles), with the aim to establish distribution of resistance betwee ...201425070092
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