| the bacterial flora of fresh and chill-stored pork: analysis by cloning and sequencing of 16s rrna genes. | the composition of the initial bacterial flora of pork and the development of the flora after storage at +4 degrees c for 4 days were analysed by amplification, cloning and sequencing of 16s rdna. a total of 122 clones were obtained, with lengths of > or =400 nucleotides and > or =95% similarity to database sequences. nineteen clones were similar to sequences in database not assigned to any genera. fourteen different genera were represented in clones from fresh meat, with 36.5% of the clones mos ... | 2003 | 12745230 |
| diversity and dynamics of communities of coagulase-negative staphylococci in traditional fermented sausages. | evaluation of composition and evolution of the coagulase-negative staphylococci (cns) communities in two traditionally fermented sausages (salsiccia and soppressata lucana) produced in basilicata, southern italy. | 2004 | 15239693 |
| development of a multiplex pcr for the identification of staphylococcus genus and four staphylococcal species isolated from food. | to develop a multiplex pcr that allows the identification of bacteria belonging to the staphylococcus genus and in particular to the species staphylococcus xylosus, s. saprophyticus, s. epidermidis and s. aureus isolated from food manufacturing plants. | 2004 | 15479426 |
| genotypic characterization of bacteria cultured from duck faeces. | to characterize the bacterial composition of mallard duck faeces and determine if novel bacterial species are present that could be utilized as potential indicators of avian faecal contamination. | 2005 | 16033461 |
| microorganisms associated with maari, a baobab seed fermented product. | a microbiological study was carried out on baobab fermented seeds (maari) obtained from 4 different production sites in burkina faso (mansila, toulfé, ouagadougou and gorgadji). a total of 390 representative isolates comprising 251 aerobic mesophilic bacteria (amb) and 139 lactic acid bacteria (lab) were isolated and identified to species level using a combination of pheno- and genotypic methods including conventional morphological analysis, carbohydrate fermentation profiling, rep-pcr ((gtg)(5) ... | 2010 | 20688411 |
| rapid and reliable identification of staphylococcus equorum by a species-specific pcr assay targeting the soda gene. | rapid and reliable identification of staphylococcus (s.) equorum was achieved by species-specific pcr assays. a set of primers targeting the manganese-dependent superoxide dismutase (soda) gene of s. equorum was designed. species-specificity of the primer set was evaluated by using a total of 112 strains (including 27 reference strains of the dsm collection), representing 26 different species of the genus staphylococcus, 3 species of the genus kocuria, and different strains of macrococcus caseol ... | 2004 | 15612627 |
| combining denaturing gradient gel electrophoresis of 16s rdna v3 region and 16s-23s rdna spacer region polymorphism analyses for the identification of staphylococci from italian fermented sausages. | separation of amplified v3 region from 16s rdna by denaturing gradient gel electrophoresis (pcr-dgge) and 16s-23s rdna intergenic spacer region polymorphism (isr-pcr) analyses were tested as tool for differentiation of staphylococcal strains commonly isolated from fermented sausages. variable v3 regions of 25 staphylococcal reference strains and 96 wild strains of species belonging to the genera staphylococcus, micrococcus and kocuria were analyzed. pcr-dgge profiles obtained were species-specif ... | 2003 | 14529185 |
| macrococcus brunensis sp. nov., macrococcus hajekii sp. nov. and macrococcus lamae sp. nov., from the skin of llamas. | eight strains of gram-positive, catalase- and oxidase-positive cocci were isolated from the skin of llamas (lama glama l.) and characterized using a polyphasic approach. these strains were assigned to the genus macrococcus on the basis of their phenotypic properties (resistance to bacitracin and sensitivity to furazolidone) and dna base content (40-42 mol% g+c). phylogenetic analysis based on 16s rdna confirmed that the strains are members of the genus macrococcus: they differed from all hithert ... | 2003 | 13130064 |
| phylogenetic study of staphylococcus and macrococcus species based on partial hsp60 gene sequences. | a 600 bp partial hsp60 gene sequence has been described previously as a novel genetic marker for species identification and phylogenetic studies within the genus staphylococcus. in the present study, the 600 bp partial hsp60 gene sequences of 40 validly described staphylococcus species and subspecies and four macrococcus species were pcr-amplified and sequenced. phylogenetic analysis revealed excellent concordance between the unrooted dendrograms based on partial hsp60 and 16s rrna gene sequence ... | 2003 | 12656157 |
| diversity, dynamics, and activity of bacterial communities during production of an artisanal sicilian cheese as evaluated by 16s rrna analysis. | the diversity and dynamics of the microbial communities during the manufacturing of ragusano cheese, an artisanal cheese produced in sicily (italy), were investigated by a combination of classical and culture-independent approaches. the latter included pcr, reverse transcriptase-pcr (rt-pcr), and denaturing gradient gel electrophoresis (dgge) of 16s rrna genes (rdna). bacterial and lactobacillus group-specific primers were used to amplify the v6 to v8 and v1 to v3 regions of the 16s rrna gene, r ... | 2002 | 11916708 |
| delimiting the genus staphylococcus through description of macrococcus caseolyticus gen. nov., comb. nov. and macrococcus equipercicus sp. nov., and macrococcus bovicus sp. no. and macrococcus carouselicus sp. nov. | four species of the newly proposed genus macrococcus, namely macrococcus caseolyticus gen. nov., comb. nov. (formerly staphylococcus caseolyticus schleifer, kilpper-bälz, fischer, faller and endl 1982, 19vp), macrococcus equipercicus sp. nov., macrococcus bovicus sp. nov. macrococcus carouselicus sp. nov., are described on the basis of a phylogenetic analysis comparing 16s rrna sequences, dna-dna liquid hybridization, dna base composition, normalized ribotype patterns, macrorestriction pattern a ... | 1998 | 9734040 |
| Biochemical characterization and FAD-binding analysis of oleate hydratase from Macrococcus caseolyticus. | A putative fatty acid hydratase gene from Macrococcus caseolyticus was cloned and expressed in Escherichia coli. The recombinant enzyme was a 68 kDa dimer with a molecular mass of 136 kDa. The enzymatic products formed from fatty acid substrates by the putative enzyme were isolated with high purity (>99%) by solvent fractional crystallization at low temperature. After the identification by GC-MS, the purified hydroxy fatty acids were used as standards to quantitatively determine specific activit ... | 2011 | 22203098 |
| catalase-positive cocci in fermented sausage: variability due to different pork breeds, breeding systems and sausage production technology. | the aim of this study was to compare the ecology of catalase-positive cocci (cpc) present in traditional fermented sausages produced using different breeds of pork, each of which was raised in two different environments and processed using two different technologies. semi-quantitative molecular methods were used to determine bacterial identities. almost all fermentations were characterised by a significant increase in cpc during the first few days of fermentation, reaching values of 10(5)-10(6) ... | 2012 | 22202871 |
| [phylogenetic diversity of culturable endophytic bacteria isolated from the populus euphratica at the disused ancient ugan river]. | the aim of this study was to identify the culturable endophytic bacteria recovered from the populus euphratica at the disused (122 years ago) ancient ugan river of middle reachs of tarim river, and to understand their phylogenetic diversity and community structure. | 2011 | 21574378 |
| diversity of the predominant spoilage bacteria in water-boiled salted duck during storage. | the spoilage microbiota in water-boiled salted duck during storage at 4 degrees c was determined using culture-dependent and independent methods. analysis of the denaturing gradient gel electrophoresis (dgge) patterns of pcr amplicons targeting the v3 region of the 16s rdna and sequencing of the bands allowed profiling of the microbiota present in the duck. community dna extracts were prepared directly from water-boiled salted duck and from culturable bacterial fractions harvested from both mrs ... | 2010 | 20629890 |
| inducibly cefoxitin-resistant macrococcus-like organism falsely identified as methicillin-resistant staphylococcus aureus on chromagar with oxacillin. | | 2010 | 20554829 |
| surface microbiota analysis of taleggio, gorgonzola, casera, scimudin and formaggio di fossa italian cheeses. | the composition of the bacterial consortia of the smear italian cheeses and their role on quality and safety is still poorly understood. the objective of this study was to identify and characterize the bacterial communities present on the surface of five traditional italian cheeses, casera valtellina, scimudin, formaggio di fossa, gorgonzola and taleggio. dgge analysis performed using total dna obtained from cheese surfaces enabled us to identify the dominant bacterial populations. bands showing ... | 2010 | 20167385 |
| staphylococcal cassette chromosome mec-like element in macrococcus caseolyticus. | macrococcus is a bacterial genus that is closely related to staphylococcus, which typically is isolated from animal skin and products. the genome analysis of multidrug-resistant macrococcus caseolyticus strain jcsc5402, isolated from chicken, previously led to the identification of plasmid pmccl2, which carries a transposon containing an unusual form of the macrococcus mec gene complex (meca(m)-mecr1(m)-meci(m)-blaz(m)). in m. caseolyticus strain jcsc7096, this mec transposon containing the mec ... | 2010 | 20086147 |
| study of microbial diversity in raw milk and fresh curd used for fontina cheese production by culture-independent methods. | the bacterial populations of raw milk employed for the production of fontina cheese in alpine farms located in different valleys and altitudes (from 700 to 2246 m above sea level) were investigated by culture independent techniques. total microbial dna was isolated from milk and curd samples and used as template in polymerase chain reaction (pcr) to study the hypervariable v3 region of the bacterial 16s rrna gene and analyzed by denaturing gradient gel electrophoresis (dgge). representative band ... | 2009 | 19232767 |
| complete genome sequence of macrococcus caseolyticus strain jcscs5402, [corrected] reflecting the ancestral genome of the human-pathogenic staphylococci. | we isolated the methicillin-resistant macrococcus caseolyticus strain jcsc5402 from animal meat in a supermarket and determined its whole-genome nucleotide sequence. this is the first report on the genome analysis of a macrococcal species that is evolutionarily closely related to the human pathogens staphylococcus aureus and bacillus anthracis. the essential biological pathways of m. caseolyticus are similar to those of staphylococci. however, the species has a small chromosome (2.1 mb) and lack ... | 2009 | 19074389 |
| identification and differentiation of staphylococcus carnosus and staphylococcus simulans by species-specific pcr assays of soda genes. | the aim of this study was to design species-specific pcr assays for rapid and reliable identification and differentiation of staphylococcus (s.) carnosus and s. simulans strains. two different sets of primers, targeting the manganese-dependent superoxide dismutase (soda) gene of s. carnosus and s. simulans, respectively, were designed. species-specificity of both sets of primers was evaluated by using 93 strains, representing 26 different species of the genus staphylococcus, 3 species of the gen ... | 2005 | 16106559 |
| culture-dependent and -independent methods to investigate the microbial ecology of italian fermented sausages. | in this study, the microbial ecology of three naturally fermented sausages produced in northeast italy was studied by culture-dependent and -independent methods. by plating analysis, the predominance of lactic acid bacteria populations was pointed out, as well as the importance of coagulase-negative cocci. also in the case of one fermentation, the fecal enterocci reached significant counts, highlighting their contribution to the particular transformation process. yeast counts were higher than th ... | 2005 | 15812029 |