| detection of a novel bovine lymphotropic herpesvirus. | degenerate pcr primers which amplify a conserved region of the dna polymerase genes of the herpesvirus family were used to provide sequence evidence for a new bovine herpesvirus in bovine b-lymphoma cells and peripheral blood mononuclear cells (pbmc). the sequence of the resultant amplicon was found to be distinct from those of known herpesvirus isolates. alignment of amino acid sequences demonstrated 70% identity with ovine herpesvirus 2, 69% with alcelaphine herpesvirus 1, 65% with bovine herp ... | 1998 | 9557713 |
| detection of two novel porcine herpesviruses with high similarity to gammaherpesviruses. | evidence for the existence of porcine gammaherpesviruses was obtained by pcr and sequence analysis. initially, samples of peripheral blood mononuclear cells (pbmc), spleens, lungs, kidneys and livers of pigs from germany and spain were tested with a pcr assay which targets conserved regions of the herpesvirus dna polymerase gene with degenerate and deoxyinosine-substituted primers. amplicons of identical sequence were obtained from one spleen and two pbmc samples. this sequence showed a high per ... | 1999 | 10211967 |
| newly recognized herpesvirus causing malignant catarrhal fever in white-tailed deer (odocoileus virginianus). | malignant catarrhal fever (mcf) was diagnosed by clinical signs and lesions in five out of six white-tailed deer (odocoileus virginianus) in a north american zoo. the clinical signs and histopathological lesions in these deer were typical of mcf. antibody to an epitope conserved among the mcf viruses was detected in the sera collected from the deer. pcr failed to amplify viral sequences from dna extracted from peripheral blood leukocytes (pbl) and/or spleens of the deer with primers specific for ... | 2000 | 10747100 |
| detection and multigenic characterization of a novel gammaherpesvirus in goats. | evidence for the existence of a caprine gammaherpesvirus was obtained by analysis of peripheral blood leucocytes of goats with pcr assays that target the herpesvirus genes encoding the glycoprotein b (gb), the dna polymerase (dpol) and the terminase (term) with degenerate and deoxyinosine-substituted primers. a contiguous 3.6 kbp sequence extending from the gb to the dpol gene was then determined with specific primers. all sequences (gb, dpol and term) showed a close relationship with the corres ... | 2001 | 11311431 |
| a novel subgroup of rhadinoviruses in ruminants. | in the course of investigating the malignant catarrhal fever (mcf) subgroup of rhadinoviruses, seven novel rhadinoviruses were identified in a variety of ruminants, including domestic sheep, bighorn sheep, bison, black-tailed deer, mule deer, fallow deer, elk and addax. based on the dna polymerase gene sequences, these newly recognized viruses clustered into a second distinct subgroup in ruminants with three members identified previously in cattle, goats and oryx. phylogenetic analysis revealed ... | 2005 | 16227223 |
| bovine lymphotropic herpesvirus and non-responsive post-partum metritis in dairy herds in the uk. | bovine lymphotropic herpesvirus (blhv) was detected for the first time in the uk in december 2005 in a dairy herd suffering from chronic, non-responsive post-partum metritis (nppm). a small-scale investigation was undertaken in order to determine whether this was an isolated case. samples of vaginal exudates or vaginal swabs were collected from cows in 13 uk dairy herds with a history of post-partum metritis that had not responded to standard treatment regimes for this condition. cows in 9/13 he ... | 2008 | 17408995 |
| quebec: detection of bovine lymphotropic herpesvirus dna in tissues of a bovine aborted fetus. | | 2010 | 21119872 |
| comparison of nested pcr and qpcr for the detection and quantitation of bohv6 dna. | nested pcr and qpcr (quantitative pcr) tests based on glycoprotein b (gb) gene were designed for detecting bovine herpesvirus 6 (bohv6) in bovine whole blood samples and wild ruminant blood clots (deer and roe-deer). this virus, commonly known as blhv (bovine lymphotropic herpesvirus) belongs to the herpesviridae family, subfamily gammaherpesvirinae and macavirus genus. dna isolated from 92 dairy cow blood samples and 69 wild ruminant clots were examined for the presence of bohv6 using nested pc ... | 2013 | 23954301 |
| detection of bovine herpesvirus type 4 antibodies and bovine lymphotropic herpesvirus in new zealand dairy cows. | to detect the presence of bovine herpesvirus (bohv) type 4 in new zealand dairy cows with clinical metritis. | 2014 | 24967608 |
| bovine lymphotropic herpesvirus detected in belgium. | | 2013 | 23687207 |