| 15 alpha-hydroxylation of 13-ethyl-gon-4-ene-3,17-dione using a hyphal fungus immobilized in calcium alginate gel beads. | calcium alginate-entrapped mycelium of penicillium raistrickii i 477 was used for the 15 alpha-hydroxylation of 13-ethyl-gon-4-ene-3,17-dione. the system was tested in 10 batches during 40 days of operation, the catalytic activity could be completely regenerated by incubation in a nutrient medium between several batches. | 1991 | 1813625 |
| an intermediary role for the tremorgenic mycotoxin tr-2 in the biosynthesis of verruculogen. | 14c-labelled compound tr-2, a tremorgenic mycotoxin, was administered to penicillium raistrickii in submerged fermentation. half of the added radiolabel was taken up by the fungus during the 60 h incubation period and the secondary metabolites subsequently isolated, principally verruculogen but also fumitremorgin b, were found to be radiolabelled. the efficiency of biosynthetic incorporation of tr-2 into verruculogen within the mycelium was at least 35%, demonstrating for the first time an inter ... | 1983 | 6626168 |
| verruculogen produced by soil fungi in england and wales. | soil fungi, including aspergillus fischeri, penicillium piceum, penicillium nigricans, and penicillium raistrickii, produced a tremorgenic toxin previously described as toxin x. chemical analysis showed that this toxin was predominantly verruculogen. | 1981 | 7316507 |
| steroid hydroxylation with free and immobilized cells of penicillium raistrickii in the presence of beta-cyclodextrin. | free and ca-alginate-immobilized cells of penicillium raistrickii i 477 were used for 15 alpha-hydroxylation of 13-ethyl-gon-4-en-3,17-dione. the product formation of both free and immobilized cells was increased in the presence of beta-cyclodextrin, in comparison with reactions carried out in the presence of methanol. application of beta-cyclodextrin led to increasing solubility of the steroid substrate. the fungus was able to utilize beta-cyclodextrin as a carbon source. | 1993 | 7763546 |
| investigations of the oxygen supply in ca-alginate beads and microcapsules loaded with penicillium raistrickii using a microelectrode. | the oxygen supply of free, ca-alginate entrapped and microencapsulated mycelia of penicillium raistrickii i 477 capable of 15 alpha-hydroxylation of 13-ethyl-gon-4-en-3,17-dione was investigated. using an oxygen microelectrode distinct gradients of oxygen within the ca-alginate beads as well as the microcapsules were detected. slope and width of the gradients were investigated in dependence on the kind of immobilization, the culture age and the cell density on or in the carrier as well as the di ... | 1993 | 8229673 |
| involvement of cytochrome p-450 in the 15alpha-hydroxylation of 13-ethyl-gon-4-ene-3,17-dione by penicillium raistrickii. | the 15alpha-hydroxylation of 13-ethyl-gon-4-ene-3,17-dione (gd) with different subcellular fractions of penicillium raistrickii i 477 was investigated. cytochrome p-450 was shown to be involved in this reaction. the steroid transformation was inhibited by carbon monoxide, metyrapone, p-cmb, iodoacetamide, n-methylmaleimide and several metal ions. the 15alpha-hydroxylase was observed to be dependent on nicotinamide-adenine dinucleotide phosphate (nadph) replaceable by naio4, and the activity was ... | 1997 | 9219926 |
| new p-terphenyl and polyketide metabolites from the sclerotia of penicillium raistrickii. | three new p-terphenyls (1-3), a new xanthone derivative (4), and two known fungal metabolites (5 and 6) have been isolated from the sclerotia of penicillium raistrickii (nrrl 2039). the structures for 3,3"-dihydroxy-6'-desmethylterphenyllin (1); 3'-demethoxy-6'-desmethyl-5'-methoxycandidusin b (2); 6'-desmethylcandidusin b (3); and 1,3,5, 6-tetrahydroxy-8-methylxanthone (4) were determined on the basis of hrms and nmr data. although compounds 1 and 4 exhibited mild antiinsectan and antibacterial ... | 1998 | 9748377 |
| an amendment of aspergillus section candidi based on chemotaxonomical evidence. | a novel 2,2'-epoxy-terphenyllin, candidusin c, in addition to the well known secondary metabolites terphenyllin, 3-hydroxyterpenyllin and chlorflavonin, has been isolated from the chemically unexplored fungus aspergillus campestris. the latter three are known secondary metabolites from aspergillus candidus and therefore a large number of aspergilli were screened for production of these compounds to see whether they could be regarded as chemotaxonomical indicators of section membership in the mon ... | 2000 | 10724184 |
| identification of fungal contamination and determination of mycotoxigenic molds by micellar electrokinetic capillary chromatography in smoked paprika. | the purpose of this work was to analyze the fungal contamination in smoked and unsmoked paprika processed from different cultivars of pepper and to investigate the ability of these and other mycotoxigenic molds to grow and synthesize mycotoxins in smoked paprika. eighteen mycotoxins were evaluated using micellar electrokinetic capillary chromatography. no relevant differences were found in fungal contamination between smoked and unsmoked paprika. the number of yeasts obtained was low, ranging fr ... | 2005 | 15830676 |
| effect of selected natural dyes in reduction on colour changes of egyptian linen textiles by fungi. | linen is the most historical egyptian textile fibre liable to fungal deterioration. fungal deterioration of dyed linen textiles may appear as undesirable different stains. in order to success in removing of fungal stains from biodeteriorated historical egyptian dyed linen textiles, it is necessary to understand the nature and causes of these stains, hence their subsequent removal. so this paper aims to investigate the effect of fungi on dyed linen textiles. in this study linen textile samples we ... | 2007 | 17867537 |
| isolation of brazilian marine fungi capable of growing on ddd pesticide. | the fungi aspergillus sydowii ce15, aspergillus sydowii ce19, aspergillus sydowii gc12, bionectria sp. ce5, penicillium miczynskii gc5, penicillium raistrickii ce16 and trichoderma sp. gc1, isolated from marine sponges geodia corticostylifera and chelonaplysylla erecta, were evaluated for their ability to grow in the presence of ddd pesticide. increasing concentrations of ddd pesticide, i.e., 5.0 mg (1.56 × 10⁻¹² mmol), 10.0 mg (3.12 × 10⁻²) mmol) and 15.0 mg (4.68 × 10⁻² mmol) in solid and liqu ... | 2011 | 20533078 |
| marine fungi aspergillus sydowii and trichoderma sp. catalyze the hydrolysis of benzyl glycidyl ether. | whole cells of the marine fungi aspergillus sydowii gc12, penicillium raistrickii ce16, p. miczynskii gc5, and trichoderma sp. gc1, isolated from marine sponges of the south atlantic ocean (brazil), have been screened for the enzymatic resolution of (±)-2-(benzyloxymethyl)oxirane (benzyl glycidyl ether; 1). whole cells of a. sydowii gc12 catalyzed the enzymatic hydrolysis of (r,s)-1 to yield (r)-1 with an enantiomeric excess (ee) of 24-46% and 3-(benzyloxy)propane-1,2-diol (2) with ee values <10 ... | 2011 | 20549284 |
| penicillium simile sp. nov. revealed by morphological and phylogenetic analysis. | the morphology of three phenetically identical penicillium isolates, collected from bioaerosol in a restoration laboratory in italy, provided macro- and microscopical characteristics that were similar though not completely ascribable to penicillium raistrickii. for this reason, a phylogenetic approach based on the dna sequencing analysis was performed to establish both the taxonomic status and the evolutionary relationships of these three peculiar isolates in relation to previously described pen ... | 2011 | 21460135 |
| biotransformation of bavachinin by three fungal cell cultures. | biotransformation of bavachinin (1) was investigated using three fungal cell cultures of aspergillus flavus atcc 30899, cunninghamella elegans cicc 40250 and penicillium raistrickii atcc 10490, respectively. two major converted products were identified by lc/ms, (1)h nmr and (13)c nmr and x-ray diffraction. two biocatalyst systems, a. flavus atcc 30899 and c. elegans cicc 40250 cell cultures, showed a great capacity of hydroxylation and two hydroxyl groups were attached at c-2″ and c-3″ position ... | 2014 | 24012108 |
| biodegradation of the pyrethroid pesticide esfenvalerate by marine-derived fungi. | esfenvalerate biodegradation by marine-derived fungi is reported here. esfenvalerate (s,s-fenvalerate) and its main metabolites [3-phenoxybenzaldehyde (pbald), 3-phenoxybenzoic acid (pbac), 3-phenoxybenzyl alcohol (pbalc), and 2-(4-chlorophenyl)-3-methylbutyric acid (clac)] were quantitatively analyzed by a validated method in triplicate experiments. all the strains (penicillium raistrickii cbmai 931, aspergillus sydowii cbmai 935, cladosporium sp. cbmai 1237, microsphaeropsis sp. cbmai 1675, ac ... | 2016 | 27381569 |
| biodegradation of methyl parathion by whole cells of marine-derived fungi aspergillus sydowii and penicillium decaturense. | seven marine fungi strains (aspergillus sydowii cbmai 934, a. sydowii cbmai 935, a. sydowii cbmai 1241, penicillium decaturense cbmai 1234, penicillium raistrickii cbmai 931, p. raistrickii cbmai 1235, and trichoderma sp. cbmai 932) were screened by their growth in the presence of methyl parathion (mp) in a solid culture medium. the strains with best growth were a. sydowii cbmai 935 and p. decaturense cbmai 1234. biodegradation reactions were performed in 10, 20 and 30d in a malt extract liquid ... | 2014 | 24955826 |
| stereoselective bioreduction of α-azido ketones by whole cells of marine-derived fungi. | seven strains of marine-derived fungi (aspergillus sclerotiorum cbmai 849, cladosporium cladosporioides cbmai 857, penicillium raistrickii cbmai 931, penicillium citrinum cbma 1186, mucor racemosus cbmai 847, beauveria felina cbmai 738, and penicillium oxalicum cbmai 1185) and terrestrial fungus penicillium chrysogenum cbma1199 were screened as catalysts for the asymmetric reduction of α-keto azides 5-8 to their corresponding β-azidophenylethanols 9-12. the marine fungi showed prelog and anti-pr ... | 2015 | 26272428 |
| pyran rings containing polyketides from penicillium raistrickii. | five new pyran rings containing polyketides, penicipyrans a-e (1-5), together with the known pestapyrone a (6), were isolated from the saline soil-derived penicillium raistrickii. their structures were determined by interpretation of nmr and hresims data. the absolute configurations of compounds 4 and 5 were established by the modified mosher's method and single-crystal x-ray diffraction analysis, respectively. these compounds possessed high structural diversity including two α-pyrones (1, 2), t ... | 2016 | 28025533 |
| [secondary metabolites from penicillium raistrickii]. | to investigate the secondary metabolites from penicillium raistrickii. | 2014 | 26080503 |
| peneciraistin c induces caspase-independent autophagic cell death through mitochondrial-derived reactive oxygen species production in lung cancer cells. | peneciraistin c (pe-c) is a novel spiroketal compound isolated from the saline soil derived fungus penicillium raistrickii. our previous study showed that pe-c exerted a potent cytotoxic effect on many kinds of cancer cell lines, especially on human lung cancer a549 cells. here, we report the anticancer mechanisms of pe-c in a variety of lung cancer cells. the results showed that pe-c induced caspase-independent autophagic cell death and elevated mitochondrial-derived reactive oxygen species lev ... | 2013 | 23952056 |
| 15α-hydroxylation of a steroid (13-ethyl-gon-4-en-3,17-dione) by penicillium raistrickii in an ionic liquid/aqueous biphasic system. | biphasic processes are used in whole-cell biotransformation to overcome the low water solubility of substrates and products as well as their inhibitory effects on the biocatalyst. commercially available [ntf(2)]- and [pf(6)]-based ionic liquids (ils) were used in a biphasic system for the 15α-hydroxylation of 13-ethyl-gon-4-en-3,17-dione by penicillium raistrickii. with the substrate at 5 g l(-1) and a volume ratio of il to buffer, buffer ph and cell density at, 1:9, 6.5, 16.8 g(dw) l(-1), respe ... | 2012 | 22829288 |
| identification and characterization of the steroid 15α-hydroxylase gene from penicillium raistrickii. | penicillium raistrickii atcc 10490 is used for the commercial preparation of 15α-13-methy-estr-4-ene-3,17-dione, a key intermediate in the synthesis of gestodene, which is a major component of third-generation contraceptive pills. although it was previously shown that a cytochrome p450 enzyme in p. raistrickii is involved in steroid 15α-hydroxylation, the gene encoding the steroid 15α-hydroxylase remained unknown. in this study, we report the cloning and characterization of the 15α-hydroxylase g ... | 2017 | 28664322 |