molecular characterisation of an isolate of dasheen mosaic virus from zantedeschia aethiopica in china and comparisons in the genus potyvirus.the complete nucleotide sequence of an isolate of dasheen mosaic virus from zantedeschia aethiopica in zhejiang province, china, was determined. the 9991 nucleotide genome was typical of the genus potyvirus and phylogenetic analysis showed it to be a member of the bean common mosaic virus subgroup. the 3'-terminal sequence, including the coat protein region, was determined for three further isolates from china and japan. variations in the length and composition of the n-terminus of the coat prot ...200111699967
zantedeschia mosaic virus causing leaf mosaic symptom in calla lily is a new potyvirus.anovel virus, zantedeschia mosaic virus (zamv-kr), causing mosaic and malformation symptoms was isolated from calla lily ( zantedeschia spp.) in korea and its biological and molecular properties were characterized. the virus was distinct from dasheen mosaic virus, an araceae-infecting potyvirus, by serological and sequence analyses. multiple alignments of the cp amino acid sequence between the virus and other potyviruses showed 51.8 to 62.1% identity. phylogenetic analyses of the cp revealed tha ...200212491097
identification and molecular characterization of zantedeschia mild mosaic virus, a new calla lily-infecting potyvirus.tissue-cultured seedlings of calla lily (zantedeschia spp.) with mild mosaic symptom were observed in taiwan. a new potyvirus, zantedeschia mild mosaic virus (zammv), was identified in the diseased plants based on the result of elisa, virion morphology and sequence data. in the host range test, zammv only infected philodendron selloum and zantedeschia spp. causing systemic mosaic and mild mosaic symptom, respectively. the 3'-terminal region of the virus was amplified by rt-pcr from total rna usi ...200515703845
differences between adenosine triphosphatases from monocotylous and dicotylous plants.the mitochondrial membrane-bound atpases of several plants were investigated. two distinct types were encountered. the mitochondrial atpases of castor bean (ricinus communis var. zanzibarensis, l.), cauliflower (brassica oleracea, l.), and scarlet runner (phaseolus coccineus, l.) were found to be inhibited by oligomycin, to have elevated molecular weights when separated from the organelles by ultrasonication and ammonium sulfate treatment and, subsequent to purification, to be cold-labile. on th ...197716659806
production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic efficient protocol for the agrobacterium tumefaciens-mediated transformation of calla lily (zantedeschia elliottiana (w. wats.) engl. cultivar 'florex gold') is described. shoot basal discs were co-cultivated with a. tumefaciens c58c1 carrying a plasmid containing neomycin phosphotransferase (nptii) and plant ferredoxin-like protein (pflp) genes. after agrobacterium co-cultivation, the shoot basal discs were exposed to 100 mg l(-1) kanamycin for selection. twenty-eight out of 260 discs (10.8% ...200717033825
priming of antimicrobial phenolics during induced resistance response towards pectobacterium carotovorum in the ornamental monocot calla lily.calla lilies are herbaceous monocotyledonous plants that are highly sensitive to pectobacterium carotovorum, the causal agent of soft-rot disease. results demonstrate that, in response to elicitation using plant defense activators, the calla lily produces elevated levels of antimicrobial phenolics and that these compounds contribute to increased resistance against p. carotovorum, as shown by reduced bacterial proliferation in elicited leaves. the polyphenolic nature of the induced compounds was ...200717994692
expression and purification of zantedeschia aethiopica agglutinin in escherichia coli.recombinant zantedeschia aethiopica agglutinin (zaa) was expressed in escherichia coli as n-terminal his-tagged fusion. after induction with isopropylthio-beta-d-galactoside (iptg), the recombinant zaa was purified by metal-affinity chromatography. the purified zaa protein was applied in anti-fungal assay and the result showed that recombinant zaa had anti-fungal activity towards leaf mold (fulvia fulva), one of the most serious phytopathogenic fungi causing significant yield loss of crops. this ...200918080841
characterization of tomato zonate spot virus, a new tospovirus in isolate of a new tospovirus species, causing concentric zoned ringspots on fruits and necrotic lesions on leaves of infected plants, was characterised based on particle morphology, host range and serological properties. the complete nucleotide sequences of large (l), medium (m), and small (s) rnas of this virus were found to contain 8919, 4945, and 3279 nts respectively. the l rna encoded the rna-dependent rna polymerase (rdrp) (2885 aa, 332.7 kda). the m rna encoded a non-structural (nsm) pr ...200818320136
differential pathogenicity and genetic diversity among pectobacterium carotovorum ssp. carotovorum isolates from monocot and dicot hosts support early genomic divergence within this taxon.the capability of pectobacterium carotovorum isolates to infect monocotyledonous plants has been previously reported; however, no full consideration was given to characterize the association between such isolates and their monocot hosts. to assess differences in aggressiveness among p. carotovorum ssp. carotovorum isolates originating from monocotyledonous or dicotyledonous plants, we used as model plants two susceptible monocot hosts, the ornamentals zantedeschia aethiopica and ornithogalum dub ...200818681897
serological comparison and molecular characterization for verification of calla lily chlorotic spot virus as a new tospovirus species belonging to watermelon silver mottle virus serogroup.abstract calla lily chlorotic spot virus (ccsv) isolated from central taiwan was recently identified as a tospovirus serologically but distantly related to watermelon silver mottle virus (wsmov). to clarify the serological relationship between the two viruses, rabbit polyclonal antibody (pab) to ccsv and mouse monoclonal antibodies (mabs) to wsmov np or ccsv np were produced in this investigation, using purified nucleocapsid protein (np) as immunogens. the pab to ccsv np reacted stronger with th ...200518943560
identification of common epitopes on a conserved region of nss proteins among tospoviruses of watermelon silver mottle virus serogroup.abstract the nss protein of watermelon silver mottle virus (wsmov) was expressed by a zucchini yellow mosaic virus (zymv) vector in squash. the expressed nss protein with a histidine tag and an additional nia protease cleavage sequence was isolated by ni(2+)-nta resins as a free-form protein and further eluted after sodium dodecyl sulfate-polyacrylamide gel electrophoresis for production of rabbit antiserum and mouse monoclonal antibodies (mabs). the rabbit antiserum strongly reacted with the ns ...200618943661
serological relationship between melon yellow spot virus and watermelon silver mottle virus and differential detection of the two viruses in cucurbits.melon yellow spot virus (mysv), a tentative member of the genus tospovirus, is considered a distinct serotype due to the lack of a serological relationship with other tospoviruses in its nucleocapsid protein (np). recently, a virus isolate collected from diseased watermelon in central taiwan (mysv-tw) was found to react with a rabbit antiserum (ras) prepared against the np of watermelon silver mottle virus (wsmov), and a monoclonal antibody (mab) prepared against the common epitope of the nss pr ...201020480192
a potyvirus p1 protein interacts with the rieske fe/s protein of its host.abstract yeast two-hybrid (y2h) screens were used to test for interactions between the p1 protein of soybean mosaic virus pinellia isolate (smv-p) and a cdna expression library of its host, the aroid pinellia ternata. of the 13 independent interacting clones identified, ten were identical and had an open reading frame predicted to encode a 23.7-kda protein closely related to the cytochrome b6/f complex rieske fe/s genes of plants. the interaction between smv-p-p1 and the mature rieske fe/s prote ...200720507538
seeking a way to promote the use of constructed wetlands for domestic wastewater treatment in developing countries.the aim of this study was to evaluate the domestic wastewater treatment efficiency as well as the survivability of commercially valuable ornamental plants in subsurface flow wetlands (ssfw) for domestic wastewater (dww) treatment in laboratory and pilot wetland studies. the laboratory scale study included five different species (zantedeschia aethiopica, strelitzia reginae, anthurium andreanum, canna hybrids and hemmerocallis dumortieri) that were evaluated in horizontal flow subsurface treatment ...201121330710
poisonous plants in new zealand: a review of those that are most commonly enquired about to the national poisons zealand has a number of plants, both native and introduced, contact with which can lead to poisoning. the new zealand national poisons centre (nznpc) frequently receives enquiries regarding exposures to poisonous plants. poisonous plants can cause harm following inadvertent ingestion, via skin contact, eye exposures or inhalation of sawdust or smoked plant matter.201223321887
shedding some light over the floral metabolism by arum lily (zantedeschia aethiopica) spathe de novo transcriptome assembly.zantedeschia aethiopica is an evergreen perennial plant cultivated worldwide and commonly used for ornamental and medicinal purposes including the treatment of bacterial infections. however, the current understanding of molecular and physiological mechanisms in this plant is limited, in comparison to other non-model plants. in order to improve understanding of the biology of this botanical species, rna-seq technology was used for transcriptome assembly and characterization. following z. aethiopi ...201424614014
characterization of pectobacterium carotovorum proteins differentially expressed during infection of zantedeschia elliotiana in vivo and in vitro which are essential for virulence.the identification of phytopathogen proteins that are differentially expressed during the course of the establishment of an infection is important to better understand the infection process. in vitro approaches, using plant extracts added to culture medium, have been used to identify such proteins, but the biological relevance of these findings for in planta infection are often uncertain until confirmed by in vivo studies. here, we compared the proteins of pectobacterium carotovorum ssp. carotov ...201627671364
genetic transformation of ornithogalum via particle bombardment and generation of pectobacterium carotovorum-resistant plants.bacterial soft rot caused by pectobacterium carotovorum subsp. carotovorum (pcc) is one of the most devastating diseases of ornithogalum species. no effective control measures are currently available to use against this pathogen; thus, introduction of resistant genes via genetic transformation into this crop is a promising approach. tachyplesin i, an antimicrobial peptide, has been shown to effectively control numerous pathogenic bacteria, including pcc. in this study, liquid-grown cell clusters ...201425438795
priming of protein expression in the defence response of zantedeschia aethiopica to pectobacterium carotovorum.the defence response of zantedeschia aethiopica, a natural rhizomatous host of the soft rot bacterium pectobacterium carotovorum, was studied following the activation of common induced resistance pathways—systemic acquired resistance and induced systemic resistance. proteomic tools were used, together with in vitro quantification and in situ localization of selected oxidizing enzymes. in total, 527 proteins were analysed by label-free mass spectrometry (ms) and annotated against the national cen ...201424822269
monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in southwestern china.the thrips-borne tospoviruses calla lily chlorotic spot virus (ccsv), tomato zonate spot virus (tzsv) and a new species provisionally named tomato necrotic spot associated virus (tnsav) infect similar crops in southwestern china. the symptoms exhibiting on virus-infected crops are similar, which is difficult for distinguishing virus species by symptomatology. the sequences of nucleocapsid proteins (nps) of ccsv, tnsav and tzsv share high degrees of amino acid identity with each other, and their ...201627121504
full-length m and l rna sequences of tospovirus isolate 2009-gzt, which causes necrotic ringspot on tomato in china.the virus isolate 2009-gzt, collected from tomato in guizhou province of china, was identified as a new member of the genus tospovirus based on its s rna sequence. because its provisional name, "tomato necrotic spot virus" (tnsv), was identical to an already existing member of the genus ilarvirus, 2009-gzt was renamed "tomato necrotic spot-associated virus" (tnsav). in this study, the full-length sequences of the genomic m and l rnas of tnsav were determined and analyzed. the m rna has 4,773 nuc ...201626887969
cloning and functional analysis of a novel ascorbate peroxidase (apx) gene from anthurium 888-bp full-length ascorbate peroxidase (apx) complementary dna (cdna) gene was cloned from anthurium andraeanum, and designated as anapx. it contains a 110-bp 5'-noncoding region, a 28-bp 3'-noncoding region, and a 750-bp open reading frame (orf). this protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting of α-helixes, β-turns, and random coils. the anapx protein showed 93%, 87%, 87%, 87%, and 86% similarities to the apx homologs from zantedeschia aethiopica, v ...201324302711
ornamental plants for micropollutant removal in wetland systems.the objective of this paper was to evaluate the efficiency of micropollutant removal, such as cu, zn, carbamazepine, and linear alkylbenzene sulfonates (las), through the use of a subsurface vertical flow constructed wetland system with ornamental plants. zantedeschia aethiopica, canna indica, carex hirta, miscanthus sinensis, and phragmites australis were selected and planted in lysimeters filled up with gravel. the lysimeters were completely saturated with synthetic wastewater (n 280 mg l(-1), ...201524798922
identification of a new tospovirus causing necrotic ringspot on tomato in china.emerging tospoviruses cause significant yield losses and quality reduction in vegetables, ornamentals, and legumes throughout the world. so far, eight tospoviruses were reported in china. tomato fruits displaying necrotic and concentric ringspot symptoms were found in guizhou province of southwest china.201425465801
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