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molecular identification of a stenotrophomonas species used in the bioassay for erythromycin in aquaculture samples.a bacterial strain isolated from aquaculture pond slurry, which was extremely sensitive to erythromycin, was used to detect erythromycin at levels as low as 0.05 micrograms ml-1 in aquaculture water, sediments and soil samples. identification of the indicator organism was attempted by 16s rrna sequencing, biochemical profile, fatty-acid analysis and polymerase chain reaction (pcr). genbank comparison showed that the 16s rrna sequence of the strain was similar to those of more than 20 copies of x ...19989727203
possible role of xanthobaccins produced by stenotrophomonas sp. strain sb-k88 in suppression of sugar beet damping-off disease.three antifungal compounds, designated xanthobaccins a, b, and c, were isolated from the culture fluid of stenotrophomonas sp. strain sb-k88, a rhizobacterium of sugar beet that suppresses damping-off disease. production of xanthobaccin a in culture media was compared with the disease suppression activities of strain sb-k88 and less suppressive strains that were obtained by subculturing. strain sb-k88 was applied to sugar beet seeds, and production of xanthobaccin a in the rhizosphere of seedlin ...199910508056
denitrification with methane as electron donor in oxygen-limited bioreactors.the microbial population from a reactor using methane as electron donor for denitrification under microaerophilic conditions was analyzed. high numbers of aerobic methanotrophic bacteria (3 10(7) cells/ml) and high numbers of acetate-utilizing denitrifying bacteria (2 10(7) cells/ml) were detected, but only very low numbers of methanol-degrading denitrifying bacteria (4 10(4) cells/ml) were counted. two abundant acetate-degrading denitrifiers were isolated which, based on 16s rrna analysis, were ...200010919339
16s ribosomal dna-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by pcr and denaturing gradient gel electrophoresis.the bacterial community in partially purified water, which is prepared by ion exchange from tap water and is used in pharmaceutical manufacturing processes, was analyzed by denaturing gradient gel electrophoresis (dgge). 16s ribosomal dna fragments, including v6, -7, and -8 regions, were amplified with universal primers and analyzed by dgge. the bacterial diversity in purified water determined by pcr-dgge banding patterns was significantly lower than that of other aquatic environments. the bacte ...200211823209
keratin degradation: a cooperative action of two enzymes from stenotrophomonas sp.a novel keratin-degrading bacterium stenotrophomonas sp. strain d-1, isolated from deer fur, produced two types of extracellular proteins: proteolytic and disulfide bond-reducing. the results on the biochemical properties suggest that this protease belongs to the serine protease, and the disulfide bond-reducing protein could be the disulfide reductase type. none of these enzymes showed keratinolytic activity independently. however, after mixing of the two enzymes, the keratinolytic activity was ...200212074595
extended spectrum beta-lactamase (esbl)-producing enterobacteriaceae: considerations for diagnosis, prevention and drug treatment.extended spectrum beta-lactamase (esbl)-producing organisms pose unique challenges to clinical microbiologists, clinicians, infection control professionals and antibacterial-discovery scientists. esbls are enzymes capable of hydrolysing penicillins, broad-spectrum cephalosporins and monobactams, and are generally derived from tem and shv-type enzymes. esbls are often located on plasmids that are transferable from strain to strain and between bacterial species. although the prevalence of esbls is ...200312558458
aerobic biodegradation of nonylphenol by cold-adapted bacteria.three strains capable of mineralizing nonylphenol as sole carbon source were isolated from a sample of contaminated soil and characterized as two pseudomonas spp. and a stenotrophomonas sp. the two pseudomonas spp. expressed characteristics typical of psychrophiles growing optimally of 10 degrees c and capable of growing at 0 degree c. the stenotrophomonas sp. was more likely psychrotrophic because it had an optimal temperature between 14 and 22 degrees c although it was not capable of growing a ...200312882175
molecular and culture-based analyses of aerobic carbon monoxide oxidizer diversity.isolates belonging to six genera not previously known to oxidize co were obtained from enrichments with aquatic and terrestrial plants. dna from these and other isolates was used in pcr assays of the gene for the large subunit of carbon monoxide dehydrogenase (coxl). coxl and putative coxl fragments were amplified from known co oxidizers (e.g., oligotropha carboxidovorans and bradyrhizobium japonicum), from novel co-oxidizing isolates (e.g., aminobacter sp. strain cox, burkholderia sp. strain lu ...200314660374
identification of novel eubacteria from spent mushroom compost (smc) waste by dna sequence typing: ecological considerations of disposal on agricultural land.a small study was undertaken to examine the microbiological characteristics of spent mushroom compost (smc), which is the major waste by-product of the mushroom industry and which is regularly disposed off by application to agricultural land. the primary aim of this study was to examine smc for the presence of faecal bacterial pathogens, including campylobacter spp., salmonella spp. and listeria monocytogenes. secondly it was desirable to quantify bacterial and fungal populations within smc, and ...200414672727
identification of genomic groups in the genus stenotrophomonas using gyrb rflp analysis.stenotrophomonas maltophilia isolates have been recovered from various clinical samples, including the respiratory tract of cystic fibrosis (cf) patients, but this organism is also widespread in nature. previously it has been shown that there is a considerable genomic diversity within s. maltophilia. the aims of our study were to determine the taxonomic resolution of restriction fragment length polymorphism (rflp) analysis of the polymerase chain reaction-amplified gyrb gene for the genus stenot ...200415039092
aerobic biotransformation of octylphenol polyethoxylate surfactant in soil microcosms.the biotransformation of octylphenol polyethoxylate surfactant (triton x-100) and bacterial communities in soil microcosms was investigated. the soil microcosms were designed to simulate real sites of bioremediation. the soil used in this study was contaminated with pesticides and alkylphenol polyethoxylate surfactants over a long period of time. the nitrogen source, (nh4)2so4 and the mineral salt basal solution were added to the microcosm and the water content was adjusted to 50% with distilled ...200415116878
analysis of tnt (2,4,6-trinitrotoluene)-inducible cellular responses and stress shock proteome in stenotrophomonas sp. ok-5.in this study, the cellular responses of stenotrophomonas sp. ok-5 to explosive 2,4,6-trinitrotoluene (tnt) have been extensively analyzed. the stress shock proteins, which might contribute to enhancing cellular resistance to tnt-mediated toxicity, were induced at different concentrations of tnt used as a substrate for cell culture of stenotrophomonas sp. ok-5 capable of utilizing tnt. proteomic analysis for 2-de of soluble protein fractions from the culture of ok-5 exposed to tnt demonstrated a ...200415486709
selenite precipitation by a rhizospheric strain of stenotrophomonas sp. isolated from the root system of astragalus bisulcatus: a biotechnological perspective.a bacterial strain (seite02), related to the species stenotrophomonas maltophilia and resistant to selenite (seiv) up to 50 mm in the growth medium, was isolated from rhizospheric soil of a selenium hyperaccumulator plant, the legume astragalus bisulcatus. the influence of seiv on the active growth of this se-tolerant bacterial strain has been investigated in oxic conditions, along with the isolate's ability to reduce selenite to elemental selenium (se(0)). interestingly, concentrations of 0.5 m ...200515661289
plant-microbe interactions: identification of epiphytic bacteria and their ability to alter leaf surface permeability.bacteria were either isolated from leaf surfaces of hedera helix or obtained from a culture collection in order to analyse their effect on barrier properties of isolated hedera and prunus laurocerasus cuticles. on the basis of the 16s rdna sequences the genera of the six bacterial isolates from hedera were identified as pseudomonas sp., stenotrophomonas sp. and achromobacter. water permeability of cuticles isolated from h. helix was measured before and after inoculation with the six bacterial st ...200515819920
characterization of a new keratin-degrading bacterium isolated from deer fur.a keratin-degrading bacterium was isolated from soil containing deer fur. an axenic culture of the keratin-degrading bacterium was obtained in liquid culture using a keratin enrichment technique. the isolated bacterium was gram negative and catalase- and oxidase-positive. transmission electron microscopic observations showed that the bacterium was rod-shaped, 1.0-1.3 microm long and 0.7 microm in diameter. phylogenetic analysis of 16s rdna revealed that the new isolate has only 90.6% homology wi ...200216233255
monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16s rrna gene-based analyses.the diversity and dynamics of bacterial populations in saint-nectaire, a raw-milk, semihard cheese, were investigated using a dual culture-dependent and direct molecular approach combining single-strand conformation polymorphism (sscp) fingerprinting and sequencing of 16s rrna genes. the dominant clones, among 125 16s rrna genes isolated from milk, belonged to members of the firmicutes (58% of the total clones) affiliated mainly with the orders clostridiales and the lactobacillales, followed by ...200717259356
identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india.lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ...200717264993
removal of cadmium ions during stationary growth phase by an extremely cadmium-resistant strain of stenotrophomonas sp.stenotrophomonas sp. cd02 was isolated from a site that previously had been contaminated with high concentrations of the heavy metals cadmium (3 mg kg(-1)) and chromium (115 mg kg(-1)). this strain was able to grow on complex (luria bertani) medium containing high concentrations of cadmium ion (up to 4 mm). additionally, it could remove up to 80% of the dissolved ions but only after reaching stationary growth phase. strain cd02 also tolerated high concentrations of other heavy metals such as chr ...200717447550
isolation and characterization of diuron-degrading bacteria from lotic surface water.the bacterial community structure of a diuron-degrading enrichment culture from lotic surface water samples was analyzed and the diuron-degrading strains were selected using a series of techniques combining temporal temperature gradient gel electrophoresis (ttge) of 16 s rdna gene v1-v3 variable regions, isolation of strains on agar plates, colony hybridization methods, and biodegradation assays. the ttge fingerprints revealed that diuron had a strong impact on bacterial community structure and ...200717450392
characterization of symbiotic and endophytic bacteria isolated from root nodules of herbaceous legumes grown in qinghai-tibet plateau and in other zones of china.qinghai-tibet plateau is the highest place in the world and the environment in that plateau is hard for animals and plants, with low temperature, low concentration of oxygen and high solar radiation. in this study, 61 root nodule isolates from vicia, oxytropis, medicago, melilotus and onobrychis species grown in qinghai-tibet plateau and in loess plateau were comparatively characterized. based upon the results of numerical taxonomy, ardra, aflp, dna-dna hybridization and 16s rdna sequencing, the ...200717541555
molecular phylogenetic diversity of bacteria and its spatial distribution in composts.to investigate microbial diversity of swine manure composts in the initial stage and the spatial distribution due to gradient effect.200717897238
cultivable bacteria associated with larval gut of prothiofos-resistant, prothiofos-susceptible and field-caught populations of diamondback moth, plutella xylostella and their potential for, antagonism towards entomopathogenic fungi and host insect nutrition.to evaluate whether the gut bacteria of insecticide-resistant, insecticide-susceptible and field-caught populations of the lepidopteran insect pest diamondback moth (dbm)--plutella xylostella (l.)--are variable and their role in host protection and nutrition.200717973916
biodegradation of p-nitrophenol and 4-chlorophenol by stenotrophomonas sp.a bacterium named lz-1 capable of utilizing high concentrations of p-nitrophenol (pnp) (up to 500 mg l(-1)) as the sole source of carbon, nitrogen and energy was isolated from an activated sludge. based on the results of phenotypic features and phylogenetic similarity of 16s rrna gene sequences, strain lz-1 was identified as a stenotrophomonas sp. other p-substituted phenols such as 4-chlorophenol (4-cp) were also degraded by strain lz-1, and both pnp and 4-cp were degraded via the hydroquinone ...200718031334
diversity and antimicrobial susceptibility of oxytetracycline-resistant isolates of stenotrophomonas sp. and serratia sp. associated with costa rican crops.to ameliorate the identification, evaluate the diversity, and determine the antimicrobial sensitivity of 19 oxytetracycline-resistant isolates of stenotrophomonas sp. and serratia sp. associated with costa rican crops.200718045439
application of a magnetotactic bacterium, stenotrophomonas sp. to the removal of au(iii) from contaminated wastewater with a magnetic separator.in this study, the feasibility of applying a magnetotactic bacterial isolate (mtb), stenotrophomonas sp. to the removal of au(iii) was investigated. biosorption experiments showed that au(iii) biosorption capacity exhibited no significant difference in the initial ph range of 1.0-5.5, while decreased more significantly in the initial ph range of 5.5-13.0. langmuir isotherm indicated that the maximum au(iii) biosorption capacity of stenotrophomonas sp. were 506, 369 and 308 mg g(-1) dry weight bi ...200818439649
biodegradation of 2,4,6-trichlorophenol in a packed-bed biofilm reactor equipped with an internal net draft tube riser for aeration and liquid circulation.for the aerobic biodegradation of the fungicide and defoliant 2,4,6-trichlorophenol (2,4,6-tcp), a bench-scale packed-bed bioreactor equipped with a net draft tube riser for liquid circulation and oxygenation (pb-alr) was constructed. to obtain a high packed-bed volume relative to the whole bioreactor volume, a high a(d)/a(r) ratio was used. reactor's downcomer was packed with a porous support of volcanic stone fragments. pb-alr hydrodynamics and oxygen mass transfer behavior was evaluated and c ...200918539387
microbial diversity of soil bacteria in agricultural field contaminated with heavy metals.in this study we evaluated the bacterial diversity in a soil sample from a site next to a chemical industrial factory previously contaminated with heavy metals. analysis of 16s rdna sequences amplified from dna directly extracted from the soil revealed 17 different bacterial types (genera and/or species). they included polyangium spp., sphingomonas spp., variovorax spp., hafina spp., clostridia, acidobacteria, the enterics and some uncultured strains. microbes able to tolerate high concentration ...200818595405
identification of novel denitrifying bacteria stenotrophomonas sp. zz15 and oceanimonas sp. yc13 and application for removal of nitrate from industrial wastewater.two novel denitrifying bacteria were successfully isolated from industrial wastewater and soil samples. using morphological, biochemical/biophysical and 16s rrna gene analyses, these two bacteria were identified as stenotrophomonas sp. zz15 and oceanimonas sp. yc13, respectively. both of these two bacteria showed efficient no(3) (-)-n removing abilities under a semi-anaerobic condition without obvious accumulation of no(2) (-)-n, n(2)o-n and nh(4) (+)-n. no(3) (-)-n removal from paper mill waste ...200919002594
cross-utilization and expression of outer membrane receptor proteins for siderophore uptake by diamondback moth plutella xylostella (lepidoptera: plutellidae) gut bacteria.siderophore production by entomo- and phytopathogens, plus the cross-utilization of these siderophores and expression of outer membrane receptor proteins (omrps) by diamondback moth (dbm) gut bacterial strains, were all examined. all the tested strains grew in the presence of 2, 2'-dipyridyl, and the brachybacterium sp. psgb10, pseudomonas sp. prgb06, and serratia marcescens flgb16 strains were found to cross-utilize the siderophores of various entomopathogens, including bacillus thuringiensis. ...200819054090
cellular and biochemical response to cr(vi) in stenotrophomonas sp.chromium (cr)-resistant bacteria isolated from a soil with 6 g kg(-1) of cr were identified based on 16s rrna gene sequence analysis as a stenotrophomonas, and designated as jd1. growth of jd1 was accompanied by transformation of cr(vi) to cr(iii) in liquid medium initially containing 300 mg l(-1) cr(vi), the maximum concentration allowing growth. jd1 produced the highest levels of a cr(vi)-binding exopolysaccharide when grown in medium with 100 mg l(-1) cr(vi). the relative exopolysaccharide mo ...200919146572
[application of biological aerated filter in treating biomass wastewater and its microbial population characteristics].biomass wastewater contains plenty of cellulose, hemicellulose, starch, saccharide, organic acid and protein. it has high cod value and leads to environment pollution and resource waste. biomass wastewater was treated in this investigate using a biological aerated filter (baf) on laboratory scale, and the effect of treatment in different hydraulic retention time (hrt) had been studied. the results show that the optimum hrt is 10 h. the value of cod and tn in effluent is 74.90 mg/l and 1.21 mg/l ...200819186816
development of an autofluorescent organophosphates-degrading stenotrophomonas sp. with dehalogenase activity for the biodegradation of hexachlorocyclohexane (hch).simultaneous biodegradation of hexachlorocyclohexane (hch) and organophosphates (ops) by a recombinant stenotrophomonas sp. was studied in the study. the broad-host-range plasmid pvgab, harboring enhanced green fluorescent protein gene (egfp) and dehalogenase genes (lina and linb), was constructed and transformed into the op-degrading strain stenotrophomonas yc-1 to get the recombinant strain yc-h. over-expression of dehalogenase (lina and linb) and enhanced green fluorescent protein (egfp) was ...200919269809
factors associated with bacteremia due to multidrug-resistant gram-negative bacilli in hematopoietic stem cell transplant recipients.the epidemiology of bacteremia developing during neutropenia has changed in the past decade, with the re-emergence of gram-negative (gn) bacteria and the development of multidrug resistance (mdr) among gn bacteria. we conducted a case-control study in order to identify factors associated with bacteremia due to multidrug-resistant gram-negative (mdrgn) isolates in hematopoietic stem cell transplant recipients. ten patients with mdrgn bacteremia were compared with 44 patients with gn bacteremia wi ...200919287908
bacterial communities in a crude oil gathering and transferring system (china).bacterial communities in crude oil and oil field production water samples from an oil gathering and transferring system in changqing oil field in china were investigated by 16s rrna denaturing gradient gel electrophoresis (dgge) analysis followed by gene cloning and sequencing. dgge profiles showed that bacterial communities are far more rich in the water samples than that in the crude oil samples, and that bacteria related to ochrobactrum sp. and stenotrophomonas sp. were detected in all crude ...200919351561
extremophile culture collection from andean lakes: extreme pristine environments that host a wide diversity of microorganisms with tolerance to uv radiation.a total of 88 bacterial strains were isolated from six andean lakes situated at altitudes ranging from 3,400 to 4,600 m above sea level: l. aparejos (4,200 m), l. negra (4,400 m), l. verde (4,460 m), l. azul (4,400 m), l. vilama (4,600 m), and salina grande (3,400 m). salinity ranged from 0.4 to 117 ppm. general diversity was determined by denaturing gradient gel electrophoresis (dgge) analysis. from the excised dgge bands, 182 bacterial sequences of good quality were obtained. gammaproteobacter ...200919495855
efficacy of bacterial consortium-aie2 for contemporaneous cr(vi) and azo dye bioremediation in batch and continuous bioreactor systems, monitoring steady-state bacterial dynamics using qpcr assays.bacterial consortium-aie2 with a capability of contemporaneous cr(vi) reduction and azo dye rv5 decolourization was developed from industrial wastewaters by enrichment culture technique. the 16s rrna gene based molecular analyses revealed that the consortium bacterial community structure consisted of four bacterial strains namely, alcaligenes sp. dma, bacillus sp. dmb, stenotrophomonas sp. dms and enterococcus sp. dme. cumulative mechanism of cr(vi) reduction by the consortium was determined usi ...200919517254
metabolism of acenaphthylene via 1,2-dihydroxynaphthalene and catechol by stenotrophomonas sp. rmsk.stenotrophomonas sp. rmsk capable of degrading acenaphthylene as a sole source of carbon and energy was isolated from coal sample. metabolites produced were analyzed and characterized by tlc, hplc and mass spectrometry. identification of naphthalene-1,8-dicarboxylic acid, 1-naphthoic acid, 1,2-dihydroxynaphthalene, salicylate and detection of key enzymes namely 1,2-dihydroxynaphthalene dioxygenase, salicylaldehyde dehydrogenase and catechol-1,2-dioxygenase in the cell free extract suggest that a ...200919543983
impact of cadmium on the bacterial communities in the gut of metaphire posthuma.the effects of cadmium (cd) contamination in soil onto the bacterial communities of the guts pooled from ten metaphire posthuma were addressed during 14 days' incubation. we found that about 50% of cd (5mg/kg, dry weight soil) in the contaminated soil was bio-accumulated into the earthworms. dna was extracted from the guts of m. posthuma and their dwelling soil irrespective of cd treatment for the analysis of the bacterial communities of guts in m. posthuma and in soil by pcr-dgge (polymerase ch ...200919747774
functional consortium for denitrifying sulfide removal process.denitrifying sulfide removal (dsr) process simultaneously converts sulfide, nitrate, and chemical oxygen demand from industrial wastewaters to elemental sulfur, nitrogen gas, and carbon dioxide, respectively. this investigation utilizes a dilution-to-extinction approach at 10(-2) to 10(-6) dilutions to elucidate the correlation between the composition of the microbial community and the dsr performance. in the original suspension and in 10(-2) dilution, the strains stenotrophomonas sp., thauera s ...201019956940
isolation of a methyl parathion-degrading strain stenotrophomonas sp. smsp-1 and cloning of the ophc2 gene.a rod-shaped, gram-negative bacterium stenotrophomonas sp. smsp-1 was isolated from the sludge of a wastewater treating system of a pesticide manufacturer. strain smsp-1 could hydrolyze methyl parathion to p-nitrophenol (pnp) and dimethyl phosphorothioate but could not degrade pnp further. strain smsp-1 was able to hydrolyze other organophosphate pesticides, including fenitrothion, ethyl parathion, fenthion, and phoxim, but not chlorpyrifos. a 4395-bp dna fragment, including an organophosphorus ...201020182770
reductive degradation of pyrazine-2-carboxylate by a newly isolated stenotrophomonas sp. hcu1.a bacterium growing on pyrazine-2-carboxylate broth was isolated, purified and identified as a strain of stenotrophomonas sp. based on polyphasic taxonomic analyses and designated as strain hcu1. 16s rrna gene sequence of strain hcu1 showed 98.7% sequence similarity with the type strain of stenotrophomonas maltophilia belonging to gammaproteobacteria. growth of strain hcu1 was demonstrated when pyrazine-2-carboxylate was used as a sole source of nitrogen. ring reduction of pyrazine-2-carboxylate ...201020217461
construction of river model biofilm for assessing pesticide effects.due to the high importance of biofilms on river ecosystems, assessment of pesticides' adverse effects is necessary but is impaired by high variability and poor reproducibility of both natural biofilms and those developed in the laboratory. we constructed a model biofilm to evaluate the effects of pesticides, consisting in cultured microbial strains, pedobacter sp. 7-11, aquaspirillum sp. t-5, stenotrophomonas sp. 3-7, achnanthes minutissima n71, nitzschia palea n489, and/or cyclotella meneghinia ...201120422166
genetic engineering of stenotrophomonas strain yc-1 to possess a broader substrate range for organophosphates.in this work, stenotrophomonas sp. strain yc-1, a native soil bacterium that produces methyl parathion hydrolase (mph), was genetically engineered to possess a broader substrate range for organophosphates (ops). a surface anchor system derived from the truncated ice nucleation protein (inpnc) from pseudomonas syringae was used to target organophosphorus hydrolase (oph) onto the cell surface of strain yc-1, reducing the potential substrate uptake limitation. the surface localization of inpnc-oph ...201020455565
degradation of microcystin-lr and rr by a stenotrophomonas sp. strain ems isolated from lake taihu, china.a bacterial strain ems with the capability of degrading microcystins (mcs) was isolated from lake taihu, china. the bacterium was tentatively identified as a stenotrophomonas sp. the bacterium could completely consume mc-lr and mc-rr within 24 hours at a concentration of 0.7 microg/ml and 1.7 microg/ml, respectively. the degradation of mc-lr and mc-rr by ems occurred preferentially in an alkaline environment. in addition, mlra gene involved in the degradation of mc-lr and mc-rr was detected in e ...201020479990
[isolation, identification, and degrading characteristics of a high-efficient pyrene-degrading bacterial strain].by using selective enrichment culture with pyrene as the sole carbon source, a pyrene-degrading bacterial strain zq5 was isolated from the oil-contaminated soil in shenfu irrigation area. the strain was identified as stenotrophomonas sp., based on its morphological, physiological, and biochemical characteristics, and similarity identification of 16s rdna sequence. the pyrene-degrading characteristics and the effects of culture condition on the degrading efficiency of the strain were investigated ...201020707122
bacterial diversity, organic pollutants and their metabolites in two aeration lagoons of common effluent treatment plant (cetp) during the degradation and detoxification of tannery wastewater.in this study, pcr-rflp and gc-ms approaches were used to characterize the bacterial diversity, organic pollutants and metabolites during the tannery wastewater treatment process at common effluent treatment plant (cetp). results revealed that the bacterial communities growing in aeration lagoon-i were dominated with escherichia sp., stenotrophomonas sp., bacillus sp. and cronobacter sp. while that of aeration lagoon-ii prevailed with stenotrophomonas sp., and burkholderiales bacterium, respecti ...201021075615
biodegradation of fenvalerate and 3-phenoxybenzoic acid by a novel stenotrophomonas sp. strain zs-s-01 and its use in bioremediation of contaminated soils.a bacterial strain zs-s-01, newly isolated from activated sludge, could effectively degrade fenvalerate and its hydrolysis product 3-phenoxybenzoic acid (3-pba). based on the morphology, physiological biochemical characteristics, and 16 s rdna sequence, strain zs-s-01 was identified as stenotrophomonas sp. strain zs-s-01 could also degrade and utilize deltamethrin, beta-cypermethrin, beta-cyfluthrin, and cyhalothrin as substrates for growth. strain zs-s-01 was capable of degrading fenvalerate ra ...201021184062
delineation of stenotrophomonas spp. by multi-locus sequence analysis and maldi-tof mass spectrometry.the genus stenotrophomonas is genetically and phenotypically heterogeneous. of the nine species now accepted, only s. maltophilia is of clinical importance. based on dna-sequences of seven house keeping genes, it encompasses genogroups of dna-similarity below 97% that predominantly comprise strains of environmental origin. therefore, in order to unravel the uneven distribution of environmental isolates within genogroups and reveal genetic relationships within the genus, there is need for an easy ...201121247714
isolation of a selected microbial consortium capable of degrading methyl parathion and p-nitrophenol from a contaminated soil site.a bacterial consortium with the ability to degrade methyl parathion and p-nitrophenol, using these compounds as the only carbon source, was obtained by selective enrichment in a medium with methyl parathion. samples were taken from moravia, medellin; an area that is highly contaminated, owing to the fact that it was used as a garbage dump from 1974 to 1982. acinetobacter sp, pseudomonas putida, bacillus sp, pseudomonas aeruginosa citrobacter freundii, stenotrophomonas sp, flavobacterium sp, prot ...201121328125
degradation of cyhalofop-butyl (cyb) by pseudomonas azotoformans strain qdz-1 and cloning of a novel gene encoding cyb-hydrolyzing esterase.cyhalofop-butyl (cyb) is a widely used aryloxyphenoxy propanoate (aopp) herbicide for control of grasses in rice fields. five cyb-degrading strains were isolated from rice field soil and identified as agromyces sp., stenotrophomonas sp., aquamicrobium sp., microbacterium sp., and pseudomonas azotoformans ; the results revealed high biodiversity of cyb-degrading bacteria in rice soil. one strain, p. azotoformans qdz-1, degraded 84.5% of 100 mg l(-1) cyb in 5 days of incubation in a flask and util ...201121534595
biodegradation of geosmin in drinking water by novel bacteria isolated from biologically active carbon.three strains of gram-negative bacteria capable of removing geosmin from drinking water were isolated from biologically active carbon and identified to be chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp. based on physio-biochemistry analysis and 16s rrna gene sequence analysis. removal efficiencies of 2 mg/l geosmin in mineral salts medium were 84.0%, 80.2% and 74.4% for chryseobacterium sp., sinorhizobium sp. and stenotrophomonas sp., respectively, while removal efficiencies of ...201121790055
c-s targeted biodegradation of dibenzothiophene by stenotrophomonas sp. nisoc-04.crude oil-contaminated soil samples were gathered across khuzestan oilfields (national iranian south oil company, nisoc) consequently experienced a screening procedure for isolating c-s targeted dibenzothiophene-biodegrading microorganisms with previously optimized techniques. among the isolates, a bacterial strain was selected due to its capability of biodegrading dibenzothiophene in a c-s targeted manner in aqueous phases and medium mostly consisting of separately biphasic water-gasoline. the ...201121750993
acceleration of nonylphenol and 4-tert-octylphenol degradation in sediment by phragmites australis and associated rhizosphere bacteria.we investigated biodegradation of technical nonylphenol (tnp) in phragmites australis rhizosphere sediment by conducting degradation experiments using sediments spiked with tnp. accelerated tnp removal was observed in p. australis rhizosphere sediment, whereas tnp persisted in unvegetated sediment without plants and in autoclaved sediment with sterile plants, suggesting that the accelerated tnp removal resulted largely from tnp biodegradation by rhizosphere bacteria. three bacterial strains, ste ...201121736332
arsenic-resistant bacteria solubilized arsenic in the growth media and increased growth of arsenic hyperaccumulator pteris vittata l.the role of arsenic-resistant bacteria (arb) in arsenic solubilization from growth media and growth enhancement of arsenic-hyperaccumulator pteris vittata l. was examined. seven arb (tolerant to 10mm arsenate) were isolated from the p. vittata rhizosphere and identified by 16s rrna sequencing as pseudomonas sp., comamonas sp. and stenotrophomonas sp. during 7-d hydroponic experiments, these bacteria effectively solubilized arsenic from the growth media spiked with insoluble feaso(4) and alaso(4) ...201121840210
removal of high concentrations of nh(3) by a combined photoreactor and biotrickling filter system.average emission levels as high as 800 ppm(v) nh(3) have often been found during the anaerobic fermentation process. at these levels, nh(3) is regarded as an environmental toxic compound. high concentrations of nh(3) gas are difficult to treat in a single treatment process, suggesting that, in terms of economic cost and treatment performance, a coupled system may be a feasible technological alternative. in the coupled tio(2) photocatalytic-biological treatment system evaluated here, the optimal ...201122175871
Reducing Sugar-Producing Bacteria from Guts of Tenebrio Molitor Linnaeus (Yellow mealworm) for Lignocellulosic Waste Minimization.The guts of Tenebrio Molitor Linnaeus (yellow mealworm) were used as inocula to isolate reducing sugar-producing bacteria during bioconversion of lignocellulose to reducing sugars in this study. Three carbon sources, i.e., carboxymethyl cellulose (CMC), filter paper (FP), and lignocellulosic waste (LIG), were specifically selected; and two types of culturing media (M1 and M2) were used. After 6 months of sequential cultivation, lignocellulose (i.e., polysaccharides) degradation of enrichments M1 ...201121869568
purification and characterization of a cold active alkaline protease from stenotrophomonas sp., isolated from kashmir, india.a psychrotolerant alkaline protease producing bacterium iiim-st045 was isolated from a soil sample collected from the thajiwas glacier of kashmir, india and identified as stenotrophomonas sp. on the basis of its biochemical properties and 16s ribosomal gene sequencing. the strain could grow well within a temperature range of 4-37°c however, showed optimum growth at 15°c. the strain was found to over-produce proteases when it was grown in media containing lactose as carbon source (157.50 u mg(-1) ...201122805828
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