| siderophores and related outer membrane proteins produced by pseudomonads isolated from eels and freshwater. | a total of 46 environmental pseudomonads, together with six type strains, were examined for their siderophore-producing activity. all strains were able to grow under iron-limiting conditions, gave orange halos in the cas agar assay, and produced hydroxamates, and some of them also produced phenolate-type compounds. bioassays showed that all strains, except pseudomonas aeruginosa, promoted growth of mutant strain arthrobacter flavescens jg-9, deficient in hydroxamate production, and some of them ... | 1992 | 1459416 |
| chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi. | linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ... | 1991 | 1657086 |
| synthetic ferrichrome analogues with growth promotion activity for arthrobacter flavescens. | two families of trihydroxamic acid analogues of ferrichrome were chemically synthesized and tested for biological activity with arthrobacter flavescens. compounds using a tertiary amine as anchor showed little activity. several compounds using tetrahedral carbon as anchor showed activity approaching or equalling that of the natural siderophore, ferrichrome. the biological activity is discussed in relation to physical and chemical properties of the analogues. | 1988 | 3196346 |
| siderophore production by vibrio vulnificus. | previous studies in our laboratory, as well as clinical evidence, have suggested that increased iron levels in the host may be important in infections caused by the halophilic pathogen vibrio vulnificus. to study iron acquisition, we induced siderophore production by growth in a low-iron medium, and biochemical testing indicated the production of both hydroxamate- and phenolate-type siderophores. the siderophores were extracted from growth filtrates with ethyl acetate (for phenolates) and phenol ... | 1983 | 6223882 |
| siderophore-mediated iron uptake in different strains of anabaena sp. | anabaena sp. strain 6411, which produces the dihydroxamate siderophore schizokinen to facilitate iron uptake, is also capable of using the related siderophore aerobactin. the two siderophores compete for the same iron transport system, but there is a markedly higher affinity for ferric schizokinen than for ferric aerobactin. the trihydroxamate siderophore ferrioxamine b is far less effective as an iron donor in this organism. anabaena sp. strain 7120 appears to be closely related to strain 6411. ... | 1983 | 6227608 |
| extracellular siderophores from aspergillus ochraceous. | a large number of iron-chelating compounds (siderophores) were isolated from supernatants of iron-deficient cultures of a mold isolate, subsequently identified as aspergillus ochraceous . siderophores in their iron chelate form were purified to homogeneity by using bio-gel p2, silica gel, and c-18 bonded silica gel (reverse-phase) columns. most of these compounds, as identified by 1h and 13c nuclear magnetic resonance spectroscopy and x-ray crystallography, belong to the ferrichrome family. the ... | 1984 | 6233261 |
| retrohydroxamate ferrichrome, a biomimetic analogue of ferrichrome. | a new synthetic analogue of ferrichrome, retrohydroxamate ferrichrome, has been examined for biological activity. although spectroscopic evidence indicates that the analogue is a weaker fe(iii) chelator than ferrichrome, retrohydroxamate ferrichrome is indistinguishable from ferrichrome in its growth factor activity for arthrobacter flavescens, and in its potency in antagonizing the antibiotic activity of albomyhcin against bacillus subtilis. it is as active as ferrichrome as a siderophore for t ... | 1984 | 6424674 |
| production and isolation of siderophores from the soil fungus epicoccum purpurascens. | a large number of iron transport agents, siderophores, which stimulated the growth of arthrobacter flavescens jg-9, were isolated during a study of the antitumor activity associated with the metabolic products of the fungus epicoccum purpurascens. the production of the siderophores was significantly enhanced in a variety of media by culture of the fungus in the near absence of ferric iron. a novel method of purification involving a carboxylic ion-exchange resin separated the siderophores into fo ... | 1981 | 6453608 |
| siderophore production by pathogenic neisseria spp. | previous studies have established the importance of iron acquisition to the growth and virulence of neisseria meningitidis and neisseria gonorrhoeae. although preliminary evidence that the neisseria spp. produce siderophores has been presented, the exact mechanism of iron acquisition has remained obscure. siderophore production by n. gonorrhoeae and n. meningitidis was induced in two different low-iron media. the iron-reactive siderophores, "gonobactin" and "meningobactin," were partially purifi ... | 1981 | 6454659 |
| malonichrome, a new iron chelate from fusarium roseum. | the predominant iron chelates, or siderochromes, produced by the fungus, fusarium roseum during culture periods up to seven days are the ester type fusarinine compounds. during longer periods of incubation, the fusarinine compounds completely disappear from the culture medium and are replaced by a new siderochrome. the new compound has been isolated, purified, and its structure determined. it is a cyclic hexapeptide containing one residue of l-alanine, two residues of glycine and three residues ... | 1980 | 7388041 |
| union of the genera microbacterium orla-jensen and aureobacterium collins et al. in a redefined genus microbacterium. | the 16s rrna gene sequences of 19 strains, 11 strains representing validated aureobacterium or microbacterium species and eight strains of non-valid species or isolates, were determined. these sequences were aligned with the sequences of other validated aureobacterium and microbacterium species and related actinobacteria. a comparative sequence analysis of 43 strains revealed that the species of the genera aureobacterium and microbacterium form a monophyletic association in which species of both ... | 1998 | 9734028 |
| siderophore mediated plutonium accumulation by microbacterium flavescens (jg-9). | uptake of plutonium and uranium mediated by the siderophore desferrioxamine-b (dfob) has been studied for the common soil aerobe microbacterium flavescens(jg-9). m. flavescens does not bind or take up nitrilotriacetic acid (nta) complexes of u(vi), fe(iii), or pu(iv) or u(vi)-dfob but does take up fe(iii)-dfob and pu(iv)-dfob. pu(iv)-dfob and fe(iii)-dfob accumulations are similar: only living and metabolically active bacteria take up these metal-siderophore complexes. the fe(iii)-dfob and pu(iv ... | 2001 | 11478246 |
| microbial growth-promotion activity of 3-hydroxymonoazine- and n-hydroxydiazine-type heterocycles. | three 3-hydroxymonoazine- and three n-hydroxydiazine-type heterocycles were tested whether they act as artificial siderophores toward aureobacterium flavescens jg-9 (atcc no. 25091). among them, 1-hydroxy-3,5,6-trimethyl-2(1h)-pyrazinone (3) showed the highest growth-promotion activity comparable to desferrioxamine b (dfb), a natural trihydroxamate siderophore, at 48.5 microm or above, followed by 1-hydroxy-5,6-dimethyl-2(1h)-pyrazinone (2), 1-hydroxy-4,6-dimethyl-2(1h)-pyrimidinone (1), and 3-h ... | 2002 | 12235861 |
| high affinity iron-uptake systems in vibrio damsela: role in the acquisition of iron from transferrin. | in this work, the high affinity iron-acquisition systems displayed by virulent and avirulent strains of vibrio damsela have been investigated. this species is an autochthonous member of marine ecosystems that can behave as an opportunistic pathogen for fish and mammals. all strains tested (i) were able to grow under the restricted conditions imposed by the iron chelators transferrin (tf) and eddha, (ii) secreted siderophores of hydroxamic type, other than aerobactin and desferal, that were able ... | 1997 | 12452589 |
| development and application of an assay for uranyl complexation by fungal metabolites, including siderophores. | an assay to detect uo(2)(2+) complexation was developed based on the chrome azurol s (cas) assay for siderophores (b. schwyn and j. b. neilands, anal. biochem. 160:47-56, 1987) and was used to investigate the ability of fungal metabolites to complex actinides. in this assay the discoloration of two dyed agars (one containing a cas-fe(3+) dye and the other containing a cas-uo(2)(2+) dye) caused by ligands was quantified. the assay was tested by using the siderophore desferrioxamine b (dfo), and t ... | 2003 | 12788768 |
| decomposition of puromycin aminoncleoside by arthrobacter flavescens. | | 1962 | 13901476 |
| synthesis, solution behavior, thermal stability, and biological activity of an fe(iii) complex of an artificial siderophore with intramolecular hydrogen bonding networks. | previously, an artificial siderophore complex, the iron(iii) complex with tris[2-[(n-acetyl-n-hydroxy)glycylamino]ethyl]amine (tage), was constructed in order to understand the effect of intramolecular hydrogen bonding interaction on the siderophore function, and its structural characterization in the solid state was reported (inorg. chem. 2001, 40, 190). in this paper, the solution behavior of the m(iii)-tage (m = fe, ga) system has been investigated using (1)h nmr, uv-vis, and fab mass spectro ... | 2004 | 15606204 |
| microbiological degradation of malodorous substances of swine waste under aerobic conditions. | phenol, p-cresol, and volatile fatty acids (vfa; acetic, propionic, isobutyric, butyric, isovaleric, and valeric acids) were used as odor indicators of swine waste. aeration of the waste allowed the indigenous microorganisms to grow and degrade these malodorous substances. the time required for degradation of these substances varied according to the waste used, and it was not necessarily related to their concentrations. using a minimal medium which contained one of the malodorous compounds as so ... | 1987 | 16347254 |
| isolation and preliminary characterization of hydroxamic acids formed by nitrogen-fixing azotobacter chroococcum b-8. | the free-living diazotroph azotobacter chroococcum b-8 responded to iron-limited growth conditions by forming hydroxamic acids and an 85,000-dalton outer membrane protein. the fe(iii)-binding hydroxamate compounds stimulated the growth of arthrobacter flavescens jg-9 and gave a positive csaky reaction for bound hydroxylamines. the hydroxamates were isolated from liquid cultures by benzyl alcohol extraction and purified by size exclusion chromatography and high-performance liquid chromatography. ... | 1989 | 16347843 |
| adsorption of microorganisms onto an artificial siderophore-modified au substrate. | the hydroxamate-type artificial siderophore, tris[2-{3-(n-acetyl-n-hydroxamino)propylamido}propyl]aminomethane (tappa) and its fe(iii) complex, fe(iii)-tappa were prepared and characterized by several spectroscopic methods. fe(iii)-tappa exhibits biological activity for the hydroxamate-type siderophore auxotrophic microorganism, microbacterium flavescens, suggesting that fe(iii)-tappa can permeate the cell membrane of the microorganism. the adsorption of the fe(iii)-siderophore complex onto a de ... | 2007 | 17890077 |
| L-Tryptophan Production by Auxotrophic and Analogue Resistant Mutants of Aureobacterium flavescens. | A number of tyrosine plus phenylalanine double auxotrophic mutants were isolated by N-methyl-N-nitro-N-nitrosoguanidine (MNNG) treatment of a locally isolated strain of Aureobacterium flavescens of which 11A(39) and 11A(17) were selected on the basis of their tryptophan production in a mineral salt medium over other isolated mutant strains. The mutational block in the aromatic amino acid biosynthetic pathway of the selected double auxotrophs were determined. By controlling pH of the production m ... | 2011 | 22084602 |
| adsorption behavior of microbes on a qcm chip modified with an artificial siderophore-fe(3+) complex. | three hydroxamate-type artificial siderophores with terminal nh(2) groups, tris[2-{3-(n-acyl-n-hydroxamino)propylamido}propyl]aminomethane (1-3, acyl-r group = me, et, and ph, respectively), and their fe(3+) complexes, 4-6, were prepared. the stability constant (log β) of 4 was estimated to be about 31 by its edta titration. the biological activities of 4-6 for microbacterium flavescens, which is a hydroxamate-type siderophore, auxotrophic gram-positive microbe, clearly indicated that they perme ... | 2011 | 22182317 |
| [Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut]. | We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping. | 2011 | 21866710 |
| microbacterium aureliae sp. nov., a novel actinobacterium isolated from aurelia aurita, the moon jellyfish. | the taxonomic position of a lemon-yellow-pigmented actinobacterium, strain jf-6t, isolated from aurelia aurita, the moon jellyfish, collected from the bay of bengal coast, kanyakumari, india, was determined using a polyphasic approach. the strain had phenotypic and chemotaxonomic properties that were consistent with its classification in the genus microbacterium. alignment of the 16s rrna gene sequence of strain jf-6t with sequences from microbacterium arthrosphaerae cc-vm-yt, microbacterium yan ... | 2016 | 27506590 |
| classification of some coryneform bacteria in a new genus aureobacterium. | chemical, biochemical and morphological data indicate a close relationship between the taxa "microbacterium liquefaciens", arthrobacter flavescens, arthrobacter terregens, "corynebacterium barkeri", curtobacterium saperdae and curtobacterium testaceum. it is proposed that the above taxa be reclassified in a new genus aureobacterium, as aureobacterium liquefaciens nom. rev., comb. nov.; aureobacterium flavescens comb. nov.; aureobacterium terregens comb. nov.; aureobacterium barkeri nom. rev., co ... | 1983 | 23194596 |