elicitor- and a23187-induced expression of wck-1, a gene encoding mitogen-activated protein kinase in wheat. | wheat cultured cells were used to study the role of ca2+ in regulating protein kinases during the induction of defense-related genes by fungal elicitor treatments. manipulation of intracellular ca2+ concentrations by treatment with calcium ionophore a23187 in the presence of high extracellular ca2+ resulted in the induction of mrna expression of wck-1, a gene encoding mitogen-activated protein (map) kinase. the induction of wck-1 mrna by a23187 did not occur when extracellular ca2+ was chelated ... | 1999 | 10527417 |
a rapid induction by elicitors of the mrna encoding ccd-1, a 14kda ca2+ -binding protein in wheat cultured cells. | intracellular ca(2+) has been implicated in the signal transduction processes during the development of the plant defense system against fungal pathogens. from wheat cultured cells that had been treated with the elicitor derived from typhula ishikariensis, the ccd-1 gene encoding a 14 kda ca(2+)-binding protein with an acidic amphiphilic feature was isolated. the ccd-1-encoded protein (ccd-1) shares homology to the c-terminal half domain of centrin, a ca(2+)-binding protein conserved in eukaryot ... | 2000 | 10890529 |
the first linkage map of the plant-pathogenic basidiomycete typhula ishikariensis. | speckled snow mold, caused by the basidiomycete typhula ishikariensis imai, is one of the most prominent winter diseases on perennial grasses and cereal crops in the northern hemisphere. the first linkage map of t. ishikariensis was constructed using a population of 93 sibling monokaryons derived from a single dikaryotic hybrid isolate that was created by a hyphal fusion of two monokaryotic parental isolates. the parental isolates were produced from a pathogenic dikaryotic isolate collected from ... | 2008 | 18356947 |
distribution of typhula spp. and typhula ishikariensis varieties in wisconsin, utah, michigan, and minnesota. | abstract snow molds are psychrophilic fungi that grow under snow cover by taking advantage of carbohydrate-depleted, dormant plants. typhula snow molds caused by typhula incarnata, t. phacorrhiza, and t. ishikariensis are the most important winter diseases of perennial grasses and winter cereals in the united states. colonized turfgrass samples with sclerotia were collected from 135 golf courses in wisconsin, utah, michigan, and minnesota in spring 2001 and 2002. species and varieties from a tot ... | 2006 | 18944047 |
ice-binding proteins from enoki and shiitake mushrooms. | fungi have developed a variety of mechanisms for tolerating cold, including production of proteins that bind to ice, as shown by their ability to slightly lower the freezing point. at present, only one of these proteins, from the snow mold typhula ishikariensis, and partial transcripts of a similar protein from shiitake mushroom, lentinula edodes, have been identified. here, we report the full sequences of ice-binding proteins from shiitake and another mushroom, the cold-adapted flammulina popul ... | 2009 | 19121299 |
paenibacillus macquariensis subsp. defensor subsp. nov., isolated from boreal soil. | two gram-variable, aerobic, motile, rod-shaped, endospore-forming bacterial strains, m4-2t and m4-1, were isolated from soil samples collected from oblast magadan, russian far east, as micro-organisms antagonistic to the psychrophilic phytopathogenic fungus typhula ishikariensis. strains m4-2t and m4-1 were identified as members of the genus paenibacillus by phenotypic and phylogenetic analyses based on 16s rrna gene sequences. the strains contained anteiso-c15:0 as the major fatty acid (63.0-64 ... | 2009 | 19605719 |
comparison of functional properties of two fungal antifreeze proteins from antarctomyces psychrotrophicus and typhula ishikariensis. | antifreeze proteins are structurally diverse polypeptides that have thermal hysteresis activity and have been discovered in many cold-adapted organisms. of these, fungal antifreeze protein has been purified and partially characterized only in a species of psychrophilic basidiomycete, typhula ishikariensis. here we report a new fungal antifreeze protein from another psychrophile, antarctomyces psychrotrophicus. we examined its biochemical properties and thermal hysteresis activity, and compared t ... | 2010 | 20030710 |
sclerotia of typhula ishikariensis biotype b (typhulaceae) from archaeological sites (4000 to 400 bp) in hokkaido, northern japan. | despite their close association with human activities, plant pathogenic fungi have rarely been found in archaeological excavations. we report here that a fungus was closely associated with human activities even in prehistoric times. sclerotium-like objects were found at historical sites (4000 to 400 bp) on the island of hokkaido, northern japan. they were spherical, 0.3-1.0 mm in diameter, and had a medulla and rind. some had leaf fragments on the surface or a protuberance that resembled emergin ... | 2010 | 21622406 |
Graminan breakdown by fructan exohydrolase induced in winter wheat inoculated with snow mold. | Fructan structures vary widely among plant species. Graminan-type fructans, extensions of sucrose through ß-(2,6)-linked fructosyl units with branches of ß-(2,1)-linked fructosyl units, accumulate in tissues of winter wheat (Triticum aestivum) during cold hardening and are metabolized under persistent snow cover. Snow molds such as Typhula ishikariensis and Microdochium nivale opportunistically infect wheat under snow cover. Snow mold-resistant wheat cultivars tend to heavily accumulate and slow ... | 2011 | 21983139 |