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detection of antibodies to toxoplasma gondii in wild animals in brazil.toxoplasmosis is a worldwide zoonosis caused by an obligate intracellular protozoan parasite, toxoplasma gondii, that affects all warm-blooded animals, including wild animals. the increased number of cases of parasitic infections is mainly due to the destruction of environmental conservation areas, which is driving wild animals out of their habitats and towards urban areas. in this study, the occurrence of t. gondii infection was investigated by the modified agglutination test (mat) in 26 differ ...201425278979
heavy metals in hair of wild canids from the brazilian cerrado.in this study, we aimed to assess whether free-ranging wild canids are exposed to heavy metals in one of the most developed and populated regions of brazil. hair of 26 wild canids (maned wolves chrysocyon brachyurus, crab-eating foxes cerdocyon thous, and hoary foxes lycalopex vetulus) from the cerrado biome in southeast brazil were analyzed by spectrophotometry to detect cadmium, chromium, and lead, and also the essential copper, iron, manganese, and zinc traces. all samples showed traces of co ...201122201044
molecular and serological detection of leishmania spp. in captive wild animals from ilha solteira, sp, brazil.leishmaniasis is a zoonotic disease that affects 12 million people worldwide. several mammalian species can serve as a reservoir for this disease. dogs are the main reservoir for visceral leishmaniasis in urban areas, which has become a serious public health concern in brazil. the aim of this study was to evaluate the presence of leishmania spp. in captive wild animals from ilha solteira, são paulo, brazil. blood and various tissues samples were collected from animals of five different species: ...201121961752
occurrence of neospora caninum and toxoplasma gondii dna in brain tissue from hoary foxes (pseudalopex vetulus) in brazil.the hoary fox (pseudalopex vetulus) is a wild canid native to brazil and is commonly found in the semiarid northeastern area living in contact with cattle. the main purpose of this study was to investigate the presence of neospora caninum and toxoplasma gondii dna in hoary foxes, in the state of paraíba, brazil. brain tissue samples were collected from 49 hoary foxes. from the samples, dna extraction and the polymerase chain reaction (pcr) were performed using specific primers for n. caninum and ...201525746974
dna extraction from hair shafts of wild brazilian felids and canids.wild felids and canids are usually the main predators in the food chains where they dwell and are almost invisible to behavior and ecology researchers. due to their grooming behavior, they tend to swallow shed hair, which shows up in the feces. dna found in hair shafts can be used in molecular studies that can unravel, for instance, genetic variability, reproductive mode and family structure, and in some species, it is even possible to estimate migration and dispersion rates in given populations ...201021174262
first identification of canine distemper virus in hoary fox (lycalopex vetulus): pathologic aspects and virus phylogeny.canine distemper virus (cdv) has been reported in several wild animal species, but there have been no reports of cdv in hoary fox (lycalopex vetulus). this paper characterizes the first case of natural cdv infection in hoary fox, including the clinical and pathologic aspects of the disease as well as the viral strain phylogeny.201020090049
serologic survey of brucellosis in captive neotropical wild carnivores in northeast brazil.abstract. this study reports the detection of antibodies against brucella abortus and b. canis in wild neotropical carnivores kept in captivity in three zoos in northeastern brazil. a total of 42 serum samples were examined, 17 from coatis (nasua nasua), eight from crab-eating raccoons (procyon cancrivorus), three from crab-eating foxes (cerdocyon thous), three from hoary foxes (lycalopex vetulus), two from little spotted cats (leopardus tigrinus), five from tayras (eira barbara), two from great ...201222779245
visceral leishmaniasis in captive wild canids in brazil.visceral leishmaniasis (vl) is endemic in belo horizonte (state of minas gerais, brazil). leishmania sp. can naturally infect several species of mammals, and the domestic dog is the most important reservoir of the disease in south america. this report describes five cases of visceral leishmaniasis in brazilian canids. among 15 animals kept in captivity in a zoo in belo horizonte (state of minas gerais, brazil), two animals, a bush dog (spheotos venaticos) and a hoary zorro (lycalopex vetulus) we ...200818556130
characterization of rabies virus isolated from canids and identification of the main wild canid host in northeastern brazil.the rabies cases in dogs and wild canids in northeastern brazil are a public and animal health problem. this paper describes the identities of the coding region of the n-gene of rabies virus (rabv) isolated in canids from northeastern brazil. the genetic tree generated using the sequence data described here divided the cluster brazilan canids into two subclusters (dog-related strains and wild canid-related strains) with identities greater than those already described. the two subclusters are sub ...200817889396
serologic evidence of leishmania infection in free-ranging wild and domestic canids around a brazilian national park.transmission of disease between wildlife, domestic animals, and humans is of great concern to conservation issues and public health. here we report on the prevalence of anti-leishmania sp. antibodies in 21 wild canids (7 chrysocyon brachyurus, 12 cerdocyon thous, and 2 lycalopex vetulus) and 74 free domestic dogs (canis familiaris) sampled around the serra do cipó national park. in dogs, the apparent prevalence was 8.1% and in wild canids it was 19% (2 crab-eating foxes, c. thous, and 2 maned wo ...200616699717
antigenic and genetic characterization of rabies viruses isolated from domestic and wild animals of brazil identifies the hoary fox as a rabies reservoir.fifty brazilian rabies viruses, collected from many different animal species and several regions of the country, were characterized by partial sequencing of the central, variable region of the p gene, a locus useful for sensitive molecular epidemiological studies. phylogenetic analysis of the sequences, which included comparison with other rabies strains recovered from throughout the americas, identified three main groups of brazilian viruses, arbitrarily designated brl-1 to brl-3. brl-1 was fou ...200516227239
ticks infesting wildlife species in northeastern brazil with new host and locality records.from september 2008 to march 2010, 397 ticks (315 larvae, 33 nymphs, 23 females, and 26 males) were collected from captive and free-living wildlife species in northeastern brazil. six tick species were identified, including amblyomma auricularium (conil) on tamandua tetradactyla (l.), amblyomma dubitatum neumann on hydrochaeris hydrochaeris (l.), nectomys rattus (pelzen) and t. tetradactyla, amblyomma parvum aragão on t. tetradactyla, amblyomma rotundatum koch on boa constrictor l., chelonoidis ...201021175080
complete genome analysis of a rabies virus isolate from brazilian wild fox.the complete genome sequence of wild-type rabies virus (rabv) isolated from a wild brazilian hoary fox (dusicyon sp.), the br-pfx1 isolate, was determined and compared with fixed rabv strains. the genome structure and organization of the br-pfx1 isolate were composed of 11,924 nt and included the five standard genes of rhabdoviruses. sequences of mrna start and stop signals for transcription were highly conserved among all structural protein genes of the br-pfx1 isolate. all amino acid residues ...200919701711
ticks (acari: ixodida) on wild carnivores in brazil.the present study reports field data of ticks infesting wild carnivores captured from july 1998 to september 2004 in brazil. additional data were obtained from one tick collection and from previous published data of ticks on carnivores in brazil. during field work, a total of 3437 ticks were collected from 89 cerdocyon thous (crab-eating fox), 58 chrysocyon brachyurus (maned wolf), 30 puma concolor (puma), 26 panthera onca (jaguar), 12 procyon cancrivorus (crab-eating raccoon), 4 speothos venati ...200516082932
wild canids, domestic dogs and their pathogens in southeast brazil: disease threats for canid conservation.wild canids are under many pressures, including habitat loss, fragmentation and disease. the current lack of information on the status of wildlife health may hamper conservation efforts in brazil. in this paper, we examined the prevalence of canine pathogens in 21 free-ranging wild canids, comprising 12 cerdocyon thous (crab-eating fox), 7 chrysocyon brachyurus (maned wolf), 2 lycalopex vetulus (hoary fox), and 70 non-vaccinated domestic dogs from the serra do cipó national park area, southeast ...201032214695
[on wild reservoirs and vectors of trypanosoma cruzi. xliv. natural infection of the field fox, dusicyon (lycalopex) vetulus (lung, 1842) by t. cruzi]. 19705499335
molecular identification of spirometra spp. (cestoda: diphyllobothriidae) in some wild animals from brazil.species of the genus spirometra are diphyllobothriid tapeworms with complex life cycles and are involved in human sparganosis, a neglected disease that affects individuals worldwide. although some species were reported in wild felids and human cases of sparganosis were described in brazil, the biology and taxonomy of these parasites are poorly understood. in the present study, samples of diphyllobothriids (eggs and/or proglottids) obtained from the stools of wild carnivores (leopardus pardalis a ...201627235572
[seasonal evaluation of mammal species richness and abundance in the "mário viana" municipal reserve, mato grosso, brasil].we evaluated seasonal species presence and richness, and abundance of medium and large sized mammalian terrestrial fauna in the "mário viana" municipal biological reserve, nova xavantina, mato grosso, brazil. during 2001, two monthly visits were made to an established transect, 2,820 m in length. records of 22 mammal species were obtained and individual footprint sequences quantified for seasonal calculation of species richness and relative abundance index (x footprints/km traveled). all 22 spec ...200618491629
wild carnivores, domestic dogs and ticks: shared parasitism in the brazilian cerrado.the increase of contact between natural and rural areas is prominent in brazil, due to agricultural activities and concern with the environmental conservation. in this context, domestic animals, wild fauna and humans are exposed to mutual exchange of parasites, microorganisms and diseases. we studied tick parasitism of wild carnivores and domestic dogs, and the environmental of questing ticks, in extensive cattle ranch areas intermingled with natural vegetation, and in a natural reserve, both in ...202032102697
isolation of rabies virus from the parotid salivary glands of foxes (pseudalopex vetulus) from paraíba state, northeastern brazil.to determine the presence of rabies virus in the parotid salivary glands, 12 road-killed rabies-positive hoary foxes (pseudoalopex vetulus) were tested by using the fluorescent antibody test (fat) and mouse inoculation test (mit). all 12 parotid salivary glands were positive for both tests, although in some cases several passages were required. the findings of this study support the importance of the hoary fox as rabies reservoir in the sem-iarid region of paraíba state, northeastern brazil.200924031385
exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus and parvovirus in the cerrado of central brazil.human population growth around protected areas increases the contact between wild and domestic animals, promoting disease transmission between them. this study investigates the exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus (cdv) and parvovirus in emas national park (enp) in the cerrado savanna of central brazil. serum samples were collected from 169 wild carnivores, including the maned wolf (chrysocyon brachyurus), crab-eating fox (cerdocyon thous), hoary f ...201627469023
wild and domestic canids and their interactions in the transmission cycles of trypanosoma cruzi and leishmania spp. in an area of the brazilian cerrado.trypanosoma cruzi and leishmania spp. are parasites that infect multiple hosts including canids, considered bioaccumulators of parasites. deforestation in the cerrado biome has resulted in the exposure of wild canids to anthropized areas, where they may establish ecological and epidemiological relationships with domestic dogs. we evaluated the infection by trypanosomatids in canids from a cerrado agroecosystem between 2013 and 2017. samples of wild canids (blood, bone marrow and skin) and dogs ( ...202033036238
rapid prototyping and inclined plane technique in the treatment of maxillofacial malformations in a fox.an approximately 9-month-old fox (pseudalopex vetulus) was presented with malocclusion and deviation of the lower jaw to the right side. orthodontic treatment was performed using the inclined plane technique. virtual 3d models and prototypes of the head were based on computed tomography (ct) image data to assist in diagnosis and treatment.201020514249
first assessment of hoary fox (lycalopex vetulus) seasonal ovarian cyclicity by non-invasive hormonal monitoring technique.reproduction is key to species survival, and reproductive physiology represents a high priority investigative area for conservation biology, as it provides a basic understanding of critical life-history traits, information that is helpful for the establishment of management strategies. here, we generated knowledge about the reproductive endocrinology of the hoary fox (lycalopex vetulus), a small canid (2.5-4 kg) endemic to open areas of the brazilian cerrado and listed in the brazilian national ...202032411373
hammondia sp. oocysts shed by a brazilian fox (lycalopex vetulus) differ from hammondia heydorni and hammondia triffittae.a brazilian fox (lycalopex vetulus) was rescued from a highway, and 16 days after maintained in captivity, the fox shed oocysts with sizes compatible with hammondia sp. and neospora caninum. dna extracted from oocysts were initially tested in two pcrs targeting the internal transcribed spacer 1 (its-1) of the rdna of hammondia heydorni and the nc-5 gene of n. caninum. a 270-bp product was visualized in the pcr for h. heydorni. no amplification was observed for n. caninum pcr. since its-1-based p ...201829781058
bartonella clarridgeiae and bartonella vinsonii subsp. berkhoffii exposure in captive wild canids in brazil.summary wild canids are potential hosts for numerous species of bartonella, yet little research has been done to quantify their infection rates in south america. we sought to investigate bartonella seroprevalence in captive wild canids from 19 zoos in são paulo and mato grosso states, brazil. blood samples were collected from 97 wild canids belonging to four different native species and three european wolves (canis lupus). indirect immunofluorescent antibody testing was performed to detect the p ...201524892580
genetic characterization of toxoplasma gondii from brazilian wildlife revealed abundant new genotypes.this study aimed to isolate and genotype t. gondii from brazilian wildlife. for this purpose, 226 samples were submitted to mice bioassay and screened by pcr based on 18s rrna sequences. a total of 15 t. gondii isolates were obtained, including samples from four armadillos (three dasypus novemcinctus, one euphractus sexcinctus), three collared anteaters (tamandua tetradactyla), three whited-lipped peccaries (tayassu pecari), one spotted paca (cuniculus paca), one oncilla (leopardus tigrinus), on ...201425426424
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