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renal hyperostotic osteodystrophy associated with unilateral renal aplasia in a captive maned wolf. 19863503153
studies on two cases of dioctophyma renale (goeze, 1782) infection in chrysocyon brachyurus (illiger). 19724271846
treatment of fibrosarcoma in a maned wolf (chrysocyon brachyurus) by rostral maxillectomy.a 12-yr-old captive intact male maned wolf (chrysocyon brachyurus) was diagnosed with a fibrosarcoma of the incisive bones. the mass was excised by rostral maxillectomy, and the wolf remained normal and on display with good function and cosmetics for 7 mo. subsequently, it became weak, ataxic, and dyspneic and was euthanatized. at necropsy, there was a small regrowth of the maxillary tumor, a metastatic mediastinal mass, and multiple metastatic lung masses, suggesting that oral fibrosarcoma in m ...200011237149
serologic response of maned wolves (chrysocyon brachyurus) to canine and canine parvovirus vaccination distemper virus.this study evaluated the immune response of 47 (22 males, 25 females) captive maned wolves (chrysocyon brachyurus) to modified-live canine parvovirus and canine distemper virus (onderstepoort and rockborn strains) vaccines. sera were collected from 33 adults and 14 pups, including five free-ranging pups captured at 1 yr of age or younger. all the adults and four captive-born pups had been vaccinated prior to this first blood collection. virus neutralization and hemagglutination-inhibition assays ...200112790399
seroprevalence of toxoplasma gondii and neospora caninum in captive maned wolves (chrysocyon brachyurus) from southeastern and midwestern regions of brazil.the main purpose of the present study was to investigate the occurrence of antibodies against t. gondii and n. caninum in captive maned wolves from brazil, considering that little information is available at the literature about infections by these parasites in this wild animal. serum samples were obtained from 59 maned wolves originated from six zoos and from one ecological reserve of the southeastern and midwestern regions of brazil. to detect igg antibodies against t. gondii, an elisa protoco ...200415262003
seroprevalences of antibodies to neospora caninum and toxoplasma gondii in zoo animals.neospora caninum is an apicomplexan parasite that causes neuromuscular disease in dogs and abortions in cattle. little is known about the prevalence of antibodies to this parasite in zoo animals. sera from 556 animals, from 13 czech and slovak zoos were tested for antibodies to n. caninum and toxoplasma gondii by indirect fluorescent antibody test. antibodies to n. caninum were found in 31 of 556 zoo animals (5.6%), representing 18 of 114 species tested: eurasian wolf (canis lupus lupus), maned ...200616387445
evaluation of homologous, heterologous, and affinity conjugates for the serodiagnosis of toxoplasma gondii and neospora caninum in maned wolves (chrysocyon brachyurus).use of serological tests in the diagnosis of infectious diseases in wild animals has several limitations, primarily the difficulty of obtaining species-specific reagents. wild canids, such as maned wolves (chrysocyon brachyurus), are highly predisposed to infection by toxoplasma gondii and, to a lesser extent, to neospora caninum. the aim of the present study was to evaluate homologous, heterologous, and affinity conjugates in enzyme-linked immunosorbent assays (elisas) and indirect fluorescent ...200516419770
a molecular phylogeny of the canidae based on six nuclear loci.we have reconstructed the phylogenetic relationships of 23 species in the dog family, canidae, using dna sequence data from six nuclear loci. individual gene trees were generated with maximum parsimony (mp) and maximum likelihood (ml) analysis. in general, these individual gene trees were not well resolved, but several identical groupings were supported by more than one locus. phylogenetic analysis with a data set combining the six nuclear loci using mp, ml, and bayesian approaches produced a mo ...200516213754
ticks (acari: ixodida) on wild carnivores in brazil.the present study reports field data of ticks infesting wild carnivores captured from july 1998 to september 2004 in brazil. additional data were obtained from one tick collection and from previous published data of ticks on carnivores in brazil. during field work, a total of 3437 ticks were collected from 89 cerdocyon thous (crab-eating fox), 58 chrysocyon brachyurus (maned wolf), 30 puma concolor (puma), 26 panthera onca (jaguar), 12 procyon cancrivorus (crab-eating raccoon), 4 speothos venati ...200516082932
populational genetic structure of free-living maned wolves (chrysocyon brachyurus) determined by proteic markers.electrophoretic analysis of presumptive twenty gene loci products was conducted in hemolisates and plasma samples of twenty-eight maned wolves (chrysocyon brachyurus) from an area in northeastern são paulo state, brazil. the area sampled was divided into three sub-areas, with the mogi-guaçu and pardo rivers regarded as barriers to the gene flow. the polymorphism degree and heterozygosity level (intralocus and average) estimated in this study were similar to those detected by other authors for ma ...200415620002
serum lipid concentrations in six canid and four ursid species in four zoos.serum lipid levels were measured in healthy captive wild canids and ursids, and the values were compared with previously published data. serum lipid levels were evaluated in blood samples collected from eight african wild dogs (lycaon pictus), three arctic foxes (alopex lagopus), nine gray wolves (canis lupus), four maned wolves (chrysocyon brachyurus), two mexican wolves (canis lupus baleiyi), nine red wolves (canis rufus), two brown bears (ursus arctos), six polar bears (ursus maritimus), six ...200415193071
extraosseous osteosarcoma in a maned wolf (chrysocyon brachyurus).a 6-yr-old maned wolf (chrysocyon brachyurus) was diagnosed with an extraosseous osteosarcoma on the lateral aspect of the right thigh. antemortem radiography revealed a calcified mass with no skeletal involvement. the mass was excised, but visible regrowth of the tumor was evident within 5 wk. histologic examination and immunohistochemistry, including staining for p53 tumor suppression gene protein, were performed on the excised mass. the maned wolf was euthanized 13 wk after the initial diagno ...200517312777
crystal structure of hemoglobin from the maned wolf (chrysocyon brachyurus) using synchrotron radiation.crystal structure of hemoglobin isolated from the brazilian maned wolf (chrysocyon brachyurus) was determined using standard molecular replacement technique and refined using maximum-likelihood and simulated annealing protocols to 1.87a resolution. structural and functional comparisons between hemoglobins from the chrysocyon brachyurus and homo sapiens are discussed, in order to provide further insights in the comparative biochemistry of vertebrate hemoglobins.200314683506
birth and mortality of maned wolves chrysocyon brachyurus (illiger, 1811) in captivity.the aims of this study were to verify the distribution of births of captive maned wolves chrysocyon brachyurus and the causes of their deaths during the period from 1980 to 1998, based on the registry of births and deaths in the international studbook for maned wolves. to determine birth distribution and average litter size, 361 parturitions were analyzed for the 1989-98 period. to analyze causes of mortality, the animals were divided into four groups: 1. pups born in captivity that died prior t ...200212185920
capillariidae eggs found in the urine of a free ranging maned wolf from argentina.the first finding of a capillariid in the urinary tract of a free ranging maned wolf (chrysocyon brachyurus) is described. the individual was an adult male attacked by dogs in the locality of cayastacito (santa fe, argentina, 31 degrees 05' s, 60 degrees 34' w). eggs found in urine measured 64.6-66.9 micrometer (mean 65.4 micrometer) x 26.9-31 micrometer (mean 29 micrometer). further studies are needed to determine whether this finding corresponds to a new capillariid species, related to c. brac ...200212118281
molecular systematics of the canidae.despite numerous systematic studies, the relationships among many species within the dog family, canidae, remain unresolved. two problems of broad evolutionary significance are the origins of the taxonomically rich canidae fauna of south america and the development in three species of the trenchant heel, a unique meat-cutting blade on the lower first molar. the first problem is of interest because the fossil record provides little evidence for the origins of divergent south american species such ...199711975336
serum concentrations of vitamin d metabolites, vitamins a and e, and carotenoids in six canid and four ursid species at four zoos.nutritional status for six captive canid species (n=34) and four captive ursid species (n=18) were analyzed. the species analyzed included: african wild dog (lycaon pictus), arctic fox (alopex lagopus), gray wolf (canis lupus), maned wolf (chrysocyon brachyurus), mexican wolf (canis lupus baleiyi), red wolf (canis rufus), brown bear (ursus arctos), polar bear (ursus maritimus), spectacled bear (tremarctos ornatus), and sun bear (ursus malayanus). diet information was collected for these animals ...200111137448
conservation value of a native forest fragment in a region of extensive agriculture.a survey of mammals and birds was carried out in a semi-deciduous forest fragment of 150 ha located in a zone of intensive agriculture in ribeirão preto, state of são paulo, south-eastern brazil. line transect sampling was used to census mammals and birds during six days, totalling 27.8 km of trails and 27.8 hours of observation. twenty mammal species were confirmed in the area (except bats and small mammals), including rare or endangered species, such as the mountain lion (puma concolor), the m ...200010959107
prevalence of gastrointestinal bacterial pathogens in a population of zoo animals.faecal prevalence of gastrointestinal bacterial pathogens, including campylobacter, escherichia coli o157:h7, salmonella, shigella, yersinia, as well as arcobacter, were examined in 317 faecal specimens from 44 animal species in belfast zoological gardens, during july-september 2006. thermophilic campylobacters including campylobacter jejuni, campylobacter coli and campylobacter lari, were the most frequently isolated pathogens, where members of this genus were isolated from 11 animal species (1 ...200818331520
cystine nephrolithiasis in an endangered canid, chrysocyon brachyurus (carnivora: canidae). 199910883330
visceral leishmaniasis in captive wild canids in brazil.visceral leishmaniasis (vl) is endemic in belo horizonte (state of minas gerais, brazil). leishmania sp. can naturally infect several species of mammals, and the domestic dog is the most important reservoir of the disease in south america. this report describes five cases of visceral leishmaniasis in brazilian canids. among 15 animals kept in captivity in a zoo in belo horizonte (state of minas gerais, brazil), two animals, a bush dog (spheotos venaticos) and a hoary zorro (lycalopex vetulus) we ...200818556130
longitudinal fecal steroid excretion in maned wolves (chrysocyon brachyurus).this study used a fecal steroid monitoring technique to evaluate reproductive cycles in male (4) and female (15) maned wolves, endangered south american canids. a radiolabeled testosterone infusion on a male revealed a fast and predominantly fecal route of excretion for this steroid. testosterone was also excreted as eight unidentified metabolites, which was not the primary form of this steroid quantified in our assays. fecal steroid concentrations (estradiol, e2; progestins, p; testosterone, t) ...19989748408
karyotype of south american pampas fox pseudalopex gymnocercus (carnivora, canidae).the karyotype of the pampas fox has 2n=74 and a nf=76. except for chrysocyon brachyurus, 2n=74 is a common diploid number for the south american canidae. this number is higher than in the vulpes group and lower than in the group of the typical canis. no 'marker chromosomes' are present in the south american canidae.19807418837
parvovirus enteritis in vaccinated juvenile bush dogs.parvovirus enteritis developed in 10 of 17 vaccinated juvenile bush dogs (speothos venaticus) from 4 litters in a 5-month period. nine dogs died. the first outbreak involved 6 of 9 bush dogs from 2 litters. each had been vaccinated with a killed feline-origin parvovirus vaccine at 11 and 14 weeks of age. the 6 affected dogs became ill at 29 weeks of age and died. the second outbreak involved a litter of 6 bush dogs. each had been vaccinated every 2 weeks starting at 5 weeks of age. two were isol ...19826890953
clinical trials with canine distemper vaccines in exotic carnivores.two types of killed canine distemper virus (cdv) vaccine and a modified-live cdv vaccine were clinically evaluated in four species of exotic carnivores. in 16 trials in which 13 red pandas (ailurus fulgens) were given the killed vaccine, only 1 animal had a virus-neutralization titer that exceeded 1:100. a red panda given modified-live cdv vaccine deemed safe for gray foxes and ferrets died of bacterial pneumonia 16 days later. there was no pathologic evidence of canine distemper in that panda. ...19836358163
[the kidney worm--dioctophyme renale--(goeze, 1782, stiles, 1901) as cause of death in a maned wolf (chryosocyon jubatus)]. 19666010492
[dioctophyma renale (dioctophymoidea, nematoda) in a maned wolf (chrysocyon brachyurus)]. 19725019466
encephalomalacia in maned wolves (chrysocyon brachyurus) associated with possible disseminated intravascular coagulation syndrome. 19873606507
chromosomal evolution of the canidae. i. species with high diploid numbers.the giemsa banding patterns of seven canid species, including the grey wolf (canis lupus), the maned wolf (chrysocyon brachyurus), the bush dog (speothos venaticus), the crab-eating fox (cerdocyon thous), the grey fox (urocyon cinereoargenteus), the bat-eared fox (otocyon megalotis), and the fennec (fennecus zerda), are presented and compared. relative to other members of canidae, these species have high diploid complements (2n greater than 64) consisting of largely acrocentric chromosomes. they ...19873568761
[seroprevalence of antibodies anti-neospora caninum and anti-toxoplasma gondii in captive wild canids].neosporosis is considered one of the main cause of abortion in dairy cattle in the world. the prevalence of neospora caninum in wild species has been studied since the coyote (canis latrans), a north american wild canid specie was discovered as definitive host of this parasite. the aim of the present study was to determine the serum prevalence of n. caninum and t. gondii in wild native canids species from brazil. serum samples of 25 crab-eating dogs (cerdocyon thous), five pampas fox (lycalopex ...200820059860
the majority of vitamin a is transported as retinyl esters in the blood of most carnivores.1. in canines and mustelides total vitamin a was 10-50 times higher compared to other species due to a high amount of retinyl esters (40-99% of total vitamin a) in blood plasma. the dominant vitamin a ester was in most species retinyl stearate. 2. in ursidae, procyonidae, viveridae and felidae, total vitamin a was much lower. when present, however, retinyl esters also represented 10-65% of total vitamin a in plasma. 3. only retinol was detected in plasma of the family, hyaenidae, and the suborde ...19901971545
cystinuria in a maned wolf.a renal calculus composed principally of the amino acid, cystine, was found in an 8-year-old male maned wolf (chrysocyon brachyurus). cystine crystals were found in the urine sediment. the renal clearance of 10 amino acids was abnormal, whereas reabsorption of others was normal. the renal clearance of cystine, lysine, ornithine, and arginine exceeded the filtered load. the renal tubular handling of glucose, phosphate, sodium, potassium, and uric acid was identical to that for the clinically norm ...1978738937
serologic evidence of leishmania infection in free-ranging wild and domestic canids around a brazilian national park.transmission of disease between wildlife, domestic animals, and humans is of great concern to conservation issues and public health. here we report on the prevalence of anti-leishmania sp. antibodies in 21 wild canids (7 chrysocyon brachyurus, 12 cerdocyon thous, and 2 lycalopex vetulus) and 74 free domestic dogs (canis familiaris) sampled around the serra do cipó national park. in dogs, the apparent prevalence was 8.1% and in wild canids it was 19% (2 crab-eating foxes, c. thous, and 2 maned wo ...200616699717
patterns of fecal gonadal hormone metabolites in the maned wolf (chrysocyon brachyurus).ex situ populations of maned wolves are not viable due to low reproductive efficiency. the objective of this study was to increase knowledge regarding the reproductive physiology of maned wolves to improve captive management. fecal samples were collected 3-5 d/wk from 12 females of various reproductive age classes (young, prime breeding and aged) and reproductive histories (conceived and raised pups, conceived but lost pups, pseudo-pregnant and unpaired). ovarian steroids were extracted from fec ...200616513162
a note on the effect of the full moon on the activity of wild maned wolves, chrysocyon brachyurus.it is well established in the scientific literature that animal prey species reduce their activity at times of high predation risk. in the case of nocturnal animals this occurs when there is a full moon; however, the response of predators to the changes in their prey behaviour is relatively unknown. two responses are possible: (1) increase in search effort to maintain food intake; or (2) decrease in distance travelled due to either: (a) an effort to conserve energy or (b) increased kill efficien ...200616814488
the seminiferous epithelium cycle and daily spermatic production in the adult maned wolf (chrysocyon brachyurus, illiger, 1811).the duration of the seminiferous epithelium cycle was estimated in adult maned wolves (chrysocyon brachyurus, illiger, 1811), by applying intratesticular injections with tritiated thymidine. the total duration of the seminiferous epithelium cycle in this species was calculated in 8.99 days. so, taking into account that approximately 4.5 cycles of the seminiferous epithelium are necessary for the whole spermatogenesis process to complete, the production of spermatozoa from one spermatogonia will ...200717157026
transferability of short tandem repeat markers for two wild canid species inhabiting the brazilian cerrado.the maned wolf (chrysocyon brachyurus) and the crab-eating fox (cerdocyon thous) are two wild-canid species found in the brazilian cerrado. we tested cross-amplification and transferability of 29 short tandem repeat primers originally developed for cattle and domestic dogs and cats on 38 individuals of each of these two species, collected in the emas national park, which is the largest national park in the cerrado region. six of these primers were successfully transferred (cssm-038, pez-05, pez- ...200617183492
immobilization of free-ranging maned wolf (chrysocyon brachyurus) with tiletamine and zolazepam in central brazil.a tiletamine hydrochloride-zolazepam hydrochloride combination was used successfully to immobilize 27 free-ranging maned wolves (chrysocyon brachyurus) at a mean dose of 2.77+/-0.56 (mean+/-sd) mg/kg. the induction time ranged from 3-15 min. animals remained immobilized for periods of 48.56 +/-12.65 min. compulsive licking, excessive salivation, muscle twitching, muscle tremors, tachypnea, and bradycardia were observed associated with the induction of the anesthesia in 13 of 27 maned wolves. mus ...200617312818
exposure of free-ranging maned wolves (chrysocyon brachyurus) to infectious and parasitic disease agents in the noël kempff mercado national park, bolivia.maned wolves (chrysocyon brachyurus) are neotropic mammals, listed as a cites appendix ii species, with a distribution south of the amazon forest from bolivia, through northern argentina and paraguay and into eastern brazil and northern uruguay. primary threats to the survival of free-ranging maned wolves include habitat loss, road kills, and shooting by farmers. an additional threat to the conservation of maned wolves is the risk of morbidity and mortality due to infectious and parasitic diseas ...200517323558
genetic variability in maned wolf based on heterologous short-tandem repeat markers from domestic dog.the maned wolf (chrysocyon brachyurus) is the largest south american canid. habitat loss and fragmentation, due to agricultural expansion and predatory hunting, are the main threats to this species. it is included in the official list of threatened wildlife species in brazil, and is also protected by iucn and cites. highly variable genetic markers such as microsatellites have the potential to resolve genetic relationships at all levels of the population structure (among individuals, demes or met ...200717624858
spotted black snake (pseudechis guttatus) envenomation in a maned wolf (chrysocyon brachyurus).envenomation by a spotted black snake (pseudechis guttatus), following multiple bites on the buccal mucosa of a captive maned wolf (chrysocyon brachyurus), caused the animal's collapse, hemolysis, rhabdomyolysis, local tissue necrosis, hepatic and renal failure, and subsequent death. the wolf died despite intensive supportive care including antivenom administration, fluid support, and a blood transfusion. gross necropsy findings included myocardial and intestinal hemorrhage, pulmonary congestion ...200717939361
comparison of serum hormone levels of captive and free-living maned wolves chrysocyon brachyurus.serum hormone levels were compared between captive and free-living maned wolves and seasonal variations of sex hormones were studied. blood samples were collected from 16 male and 26 female adult animals from brazilian zoos, and from 30 male and 24 female free-living adults to determine serum progesterone and testosterone by radioimmunoassay. serum testosterone concentrations varied (p < 0.05) across seasons for 16 captive males, being higher in autumn (2184.7 +/- 355.1 pg/ml) than in summer (10 ...200818297198
prevalence of crown trauma in free-ranging maned wolves (chrysocyon brachyurus) in central brazil.nineteen free-ranging maned wolves (chrysocyon brachyurus) were captured in central brazil from april through november 2003. oral cavity examination revealed 34 teeth with crown trauma in twelve animals. canine teeth were most commonly affected (44.2%), followed by premolar (29.4%) and incisor teeth (26.5%). trauma to maxillary teeth (67.7%) was more frequent compared with mandibular teeth (32.3 %). the majority of dental fractures were located at the cuspid portion of the crown (56.0%). slab fr ...200718309856
cytogenetic studies and karyotype nomenclature of three wild canid species: maned wolf (chrysocyon brachyurus), bat-eared fox (otocyon megalotis) and fennec fox (fennecus zerda).we have analysed the chromosomes of three wild and endangered canid species: the maned wolf (chrysocyon brachyurus), the bat-eared fox (otocyon megalotis) and the fennec fox (fennecuszerda) using classical and molecular cytogenetic methods. for the first time detailed and encompassing descriptions of the chromosomes are presented including the chromosomal assignment of nucleolar organizer regions and the 5s rrna gene cluster. we propose a karyotype nomenclature with ideograms including more than ...200818544923
a simple ductal mammary papilloma in a male maned wolf (chrysocyon brachyurus).a 1-cm-diameter nodule was identified in the left inguinal mammary gland of a 9-year-old male maned wolf (chrysocyon brachyurus). the mass was surgically excised and examined histologically. microscopically, the neoplasm consisted of papillary proliferations of epithelial cells on well-defined fibrovascular stalks. a myoepithelial layer was located between the single layer of epithelial cells and the fibrovascular stalk. this histologic appearance was compatible with a diagnosis of simple ductal ...200919139520
serologic survey of infectious diseases in populations of maned wolf (chrysocyon brachyurus) and crab-eating fox (cerdocyon thous) from aguas emendadas ecological station, brazil.domestic dogs are reservoirs for many infectious diseases and may represent a potential source of infection for wild canid populations. a serologic investigation of antibodies to toxoplasma gondii, neospora caninum, brucella abortus, and leptospira spp. was conducted on three maned wolves (chrysocyon brachyurus) and seven crab-eating foxes (cerdocyon thous), all free-living, at the aguas emendadas ecological station (esecae), federal district, brazil, between february and october 2006. out of th ...201323505716
pathogens of wild maned wolves (chrysocyon brachyurus) in brazil.the maned wolf, chrysocyon brachyurus, is an endangered neotropical canid that survives at low population densities. diseases are a potential threat for its conservation but to date have been poorly studied. we performed clinical evaluations and investigated the presence of infectious diseases through serology and coprologic tests on maned wolves from galheiro natural private reserve, perdizes city, minas gerais state, southeastern brazil. fifteen wolves were captured between 2003 and 2008. we f ...201223060508
hematology and blood chemistry parameters differ in free-ranging maned wolves (chrysocyon brachyurus) living in the serra da canastra national park versus adjacent farmlands, brazil.there has been growing interest in the specific impacts of anthropogenic factors on the health of wildlife. this study examined hematology and serum chemistry status of a prominent carnivore, the maned wolf (chrysocyon brachyurus), living in, on the boundaries to, or on adjacent farmlands to the serra da canastra national park, brazil. twenty-eighty wolves were captured, and values were compared 1) between subadults (n=8 animals) and adults (n=20 animals), 2) males (n=12 animals) and females (n= ...200919204338
first observations of nest attendance behavior by wild maned wolves, chrysocyon brachyurus.in many species of canids the male's role in reproduction extends to providing extensive or some parental care to his offspring. maned wolves are a monogamous canid species whose males have been observed providing parental care to their offspring in captivity, but no field observations exist. we observed a wild pair of maned wolves at their nest site in a period soon after the female had given birth for a total period of 65 days. we made five observation sessions with an average of 3-4 days each ...200919358320
behavioral and physiologic responses to environmental enrichment in the maned wolf (chrysocyon brachyurus).the ex situ population of maned wolves is not self-sustaining due to poor reproduction, caused primarily by parental incompetence. studies have shown that environmental enrichment can promote natural parental behaviors in zoo animals. the objective of this study was to determine the effects of environmental enrichment on behavioral and physiological responses of maned wolves. during an 8-week experimental period, daily behavior observations and fecal sample collection were conducted on four adul ...200719360584
synovial cell sarcoma in a captive maned wolf (chrysocyon brachyurus). 200919377091
relaxin concentrations in serum and urine of endangered and crazy mixed-up species.the human population explosion has pushed many mammalian wildlife species to the brink of extinction. conservationists are increasingly turning to captive breeding as a means of preserving the gene pool. we previously reported that serum immunoactive relaxin provided a reliable means of distinguishing between true and pseudopregnancy in domestic dogs, and this method has since been found to be a reliable indicator of true pregnancy in endangered asian and african elephants and sumatran rhinocero ...200919416182
first record of entodiniomorph ciliates in a carnivore, the maned wolf (chrysocyon brachyurus), from brazil.the entodiniomorph ciliates (ciliophora: entodiniomorphida) are endosymbiotes widely found in the intestines of herbivorous mammals. these commensals commonly occur in the artiodactyla and perissodactyla and have also been described in the proboscidea, primates, rodentia, and diprotodontia. this study reports the first finding of a ciliate in a member of order carnivora, the maned wolf (chrysocyon brachyurus). fecal samples from wild and captive maned wolves were screened using ethyl acetate sed ...200919569492
cardiac evaluation of clinically healthy captive maned wolves (chrysocyon brachyurus).the objective of this project was to determine radiographic vertebral heart sizes and electrocardiographic (ecg) and echocardiographic measurements in healthy anesthetized maned wolves (chrysocyon brachyurus). the wolves, housed at the white oak conservation and smithsonian national zoo conservation and research centers, were being anesthetized for annual examinations. cardiac auscultation, thoracic radiographs, a standard 6-lead ecg, and echocardiography were performed on the wolves while they ...200919746863
advances in reproductive science for wild carnivore conservation.knowledge about reproduction is critical for predicting the viability of wildlife populations in nature and for managing breeding programmes in captivity. intensive species-based studies are the priority, because reproductive mechanisms are extraordinarily diverse, even within the same taxonomic family. carnivores deserve more attention as such species are highly vulnerable to environmental change and human persecution. the present review provides contemporary illustrations of how reproductive s ...200919754535
a comparative approach to the study of keeper-animal relationships in the zoo.research on intensively farmed animals over the past 25 years has shown that human-animal interactions, by affecting the animal's fear of humans, can markedly limit the productivity and welfare of farm animals. this article begins to explore some of the factors that need to be considered to investigate keeper-animal relationships (kars) in the zoo. in the mid-1990s, a large body of multi-institutional data on zookeepers and animals was collected from 46 zoos. using standardized questionnaires, 8 ...200919885915
non-invasive measurement of thyroid hormone in feces of a diverse array of avian and mammalian species.we developed and validated a non-invasive thyroid hormone measure in feces of a diverse array of birds and mammals. an i(131) radiolabel ingestion study in domestic dogs coupled with high pressure liquid chromatography (hplc) analysis, showed that peak excretion in feces occurred at 24-48h post-ingestion, with i(131)-labelled thyroid hormone metabolites excreted primarily as triiodothyronine (t3) and relatively little thyroxine (t4), at all excretion times examined. the immunoreactive t3 profile ...201020412809
dna extraction from hair shafts of wild brazilian felids and canids.wild felids and canids are usually the main predators in the food chains where they dwell and are almost invisible to behavior and ecology researchers. due to their grooming behavior, they tend to swallow shed hair, which shows up in the feces. dna found in hair shafts can be used in molecular studies that can unravel, for instance, genetic variability, reproductive mode and family structure, and in some species, it is even possible to estimate migration and dispersion rates in given populations ...201021174262
myiasis by screw worm cochliomyia hominivorax (coquerel) (diptera: calliphoridae) in a wild maned wolf chrysocyon brachyurus (mammalia: canidae), in bras+¡lia, brazil.in april 2009, a wild maned wolf, chrysocyon brachyurus, was captured in an area of cerrado in bras+¡lia, df, brazil, with screw worm maggots in external wounds. fifty larvae were bred in the laboratory and eight adults of cochliomyia hominivorax (coquerel) emerged 10 days after pupation. this is the first report of a myiasis by c. hominivorax in a free-living maned wolf in brazil.201121437499
[studies on rabies in brazil. i. occurrence of rabies in the pampas wolf (chrysocyon brachyurus)]. 19684230925
cystinuria in the maned wolf of south america.of 42 maned wolves in zoos or live-trapped in brazil, 34 had excessive cystine in their urine. renal clearance studies of five of the affected wolves revealed a variable defect for the reabsorption of cystine and dibasic amino acids. the renal tubular handling of other solutes including glucose, phosphate, sodium, potassium, and uric acid was considered normal. urinary calculi composed of cystine were found in four wolves and proved fatal in three of them. with the exception of the high incidenc ...19817233184
structure of greyhound hemoglobin: origin of high oxygen affinity.this study presents the crystal structure of greyhound hemoglobin (grhb) determined to 1.9 å resolution. grhb was found to crystallize with an α(1)β(1) dimer in the asymmetric unit and belongs to the r2 state. oxygen-affinity measurements combined with the fact that grhb crystallizes in the r2 state despite the high-salt conditions used for crystallization strongly indicate that grhb can serve as a model high-oxygen-affinity hemoglobin (hb) for higher mammals, especially humans. structural analy ...201121543841
behavioral responses of maned wolves (chrysocyon brachyurus, canidae) to different categories of environmental enrichment stimuli and their implications for successful reintroduction.the maned wolf (chrysocyon brachyurus, illiger, 1815, canidae) is a threatened species that inhabits the cerrados of brazil, argentina, peru, bolivia, paraguay, and uruguay. captive maned wolves could be potentially used in reintroduction programs for species conservation; however, it is necessary that their behavior and cognitive abilities are conserved. environmental enrichment is a tool used to stimulate captive animals and maintain a natural behavioral repertoire. to compare the variation of ...201121796672
mandibular morphometry applied to anesthetic blockage in the maned wolf (chrysocyon brachyurus).chrysocyon brachyurus (maned wolf) is the biggest south american canid and has a high frequency of dental injuries, both in the wild and in captivity. thus, veterinary procedures are necessary to preserve the feeding capacity of hundreds of captive specimens worldwide. the aim of this study was to investigate the mandibular morphometry of the maned wolf with emphasis on the establishment of anatomic references for anesthetic block of the inferior alveolar and mental nerves. therefore, 16 measure ...201627010268
population structure of mtdna variation due to pleistocene fluctuations in the south american maned wolf (chrysocyon brachyurus, illiger, 1815): management units for conservation. 201528158644
Resource selection and its implications for wide-ranging mammals of the brazilian cerrado.Conserving animals beyond protected areas is critical because even the largest reserves may be too small to maintain viable populations for many wide-ranging species. Identification of landscape features that will promote persistence of a diverse array of species is a high priority, particularly, for protected areas that reside in regions of otherwise extensive habitat loss. This is the case for Emas National Park, a small but important protected area located in the Brazilian Cerrado, the world' ...201122205984
molecular and serological detection of leishmania spp. in captive wild animals from ilha solteira, sp, brazil.leishmaniasis is a zoonotic disease that affects 12 million people worldwide. several mammalian species can serve as a reservoir for this disease. dogs are the main reservoir for visceral leishmaniasis in urban areas, which has become a serious public health concern in brazil. the aim of this study was to evaluate the presence of leishmania spp. in captive wild animals from ilha solteira, são paulo, brazil. blood and various tissues samples were collected from animals of five different species: ...201121961752
heavy metals in hair of wild canids from the brazilian cerrado.in this study, we aimed to assess whether free-ranging wild canids are exposed to heavy metals in one of the most developed and populated regions of brazil. hair of 26 wild canids (maned wolves chrysocyon brachyurus, crab-eating foxes cerdocyon thous, and hoary foxes lycalopex vetulus) from the cerrado biome in southeast brazil were analyzed by spectrophotometry to detect cadmium, chromium, and lead, and also the essential copper, iron, manganese, and zinc traces. all samples showed traces of co ...201122201044
seasonal aspects of reproductive physiology in captive male maned wolves (chrysocyon brachyurus, illiger 1815).the aim of the study was to evaluate the seasonality of andrological characteristics and hormonal profile of captive maned wolves (chrysocyon brachyurus, illiger 1811). three adult males were evaluated from the companhia brasileira de metalurgia e mineração scientific breeding center in araxá, mg, brazil, over 13 months. semen was collected 2-3 times weekly and analysed. scrotal circumference, biometrics and testicular volume were measured. stool samples were collected 2-3 times weekly to analys ...201223279512
rangelia vitalii in a free-ranging maned wolf (chrysocyon brachyurus) and co-infections.an adult free-ranged female maned wolf was rescued from a periurban area subject to anthropogenic disturbances in the minas gerais, brazil. the animal presented poor body condition and anemia. the clinical condition rapidly deteriorated culminating in dead and a necropsy was performed. the main gross lesions were marked anemia and blood content in the intestines accompanied by many types of parasites. the protozoa rangelia vitalii was identified by histopathological analysis predominantly within ...201627761403
genetic characterization of toxoplasma gondii from brazilian wildlife revealed abundant new genotypes.this study aimed to isolate and genotype t. gondii from brazilian wildlife. for this purpose, 226 samples were submitted to mice bioassay and screened by pcr based on 18s rrna sequences. a total of 15 t. gondii isolates were obtained, including samples from four armadillos (three dasypus novemcinctus, one euphractus sexcinctus), three collared anteaters (tamandua tetradactyla), three whited-lipped peccaries (tayassu pecari), one spotted paca (cuniculus paca), one oncilla (leopardus tigrinus), on ...201425426424
parasites of domestic and wild canids in the region of serra do cipó national park, brazil.over recent decades, diseases have been shown to be important causes of extinctions among wild species. greater emphasis has been given to diseases transmitted by domestic animals, which have been increasing in numbers in natural areas, along with human populations. this study had the aim of investigating the presence of intestinal helminths in wild canids (maned wolf, chrysocyon brachyurus, and crab-eating fox, cerdocyon thous) in the serra do cipó national park (43-44º w and 19-20º s) and endo ...201223070438
bartonella clarridgeiae and bartonella vinsonii subsp. berkhoffii exposure in captive wild canids in brazil.summary wild canids are potential hosts for numerous species of bartonella, yet little research has been done to quantify their infection rates in south america. we sought to investigate bartonella seroprevalence in captive wild canids from 19 zoos in são paulo and mato grosso states, brazil. blood samples were collected from 97 wild canids belonging to four different native species and three european wolves (canis lupus). indirect immunofluorescent antibody testing was performed to detect the p ...201524892580
exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus and parvovirus in the cerrado of central brazil.human population growth around protected areas increases the contact between wild and domestic animals, promoting disease transmission between them. this study investigates the exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus (cdv) and parvovirus in emas national park (enp) in the cerrado savanna of central brazil. serum samples were collected from 169 wild carnivores, including the maned wolf (chrysocyon brachyurus), crab-eating fox (cerdocyon thous), hoary f ...201627469023
hemorrhagic and necrotizing hepatitis associated with administration of a modified live canine adenovirus-2 vaccine in a maned wolf (chrysocyon brachyurus).a 15-yr-old, female, maned wolf (chrysocyon brachyurus) was euthanized after presenting semicomatose with severe, uncontrolled frank hemorrhage from her rectum 6 days following a routine physical examination and vaccination. histopathology indicated severe hemorrhagic and necrotizing hepatitis with intranuclear basophilic inclusion bodies in the liver that were thought to be consistent with adenoviral infection. further classification by polymerase chain reaction, immunohistochemical staining, v ...201222779244
clostridium difficile and clostridium perfringens from wild carnivore species in brazil.despite some case reports, the importance of clostridium perfringens and clostridium difficile for wild carnivores remains unclear. thus, the objective of this study was to identify c. perfringens and c. difficile strains in stool samples from wild carnivore species in brazil. a total of 34 stool samples were collected and subjected to c. perfringens and c. difficile isolation. suggestive colonies of c. perfringens were then analyzed for genes encoding the major c. perfringens toxins (alpha, bet ...201424979683
microbiota and anthropic interference on antimicrobial resistance profile of bacteria isolated from brazilian maned-wolf (chrysocyon brachyurus).both the study of brazilian wild mammal fauna and the conditions that foster the preservation of endangered species, such as brazilian maned-wolf (chrysocyon brachyurus), in wild life are of extreme importance. in order to study the resistance profile of microbiota bacterial colonizing brazilian maned-wolf, this work investigated samples from eight male captive and free roaming animals originating from different brazilian geographical regions. samples for microbiological purposes were collected ...201324688529
transmissibility of leishmania infantum from maned wolves (chrysocyon brachyurus) and bush dogs (speothos venaticus) to lutzomyia longipalpis.leishmania (leishmania) infantum is the cause of visceral leishmaniasis in the americas. the disease is transmitted mostly through the bite of the invertebrate vector, the phlebotomine lutzomyia longipalpis in the new world. although the domestic dog is considered the most important reservoir of the disease, other mammalian, including wildlife, are susceptible to infection. the goal of this study was to perform xenodiagnosis to evaluate the capacity of naturally infected maned wolves (chrysocyon ...201526342623
seroprevalence of nine leptospira interrogans serovars in wild carnivores, ungulates, and primates from a zoo population in a metropolitan region of chile.serum samples from 130 individuals representing 42 species of carnivores, ungulates, and primates from a population of captive mammals in metropolitan region in chile were tested for antibodies against nine serovars of leptospira interrogans using the microscopic agglutination test. ten percent of the animals were seropositive to one or more serovars. seroprevalence was significantly higher in ungulates (20.4%) compared to carnivores (3.8%) and primates (3.4%). there were no significant differen ...201526667533
oslerus osleri (cobbold, 1876) infection in maned wolf (chrysocyon brachyurus, illiger, 1815).oslerus osleri is a small nematode that infects the respiratory tract of domestic and wild canids and is responsible for causing chronic nodular tracheobronchitis. this paper aims to report a case of parasitism by o. osleri in a free-living maned wolf (chrysocyon brachyurus) that was struck by a motor vehicle. fecal samples were collected, and the presence of spiral larvae, with "s"-shaped tails, was observed on flotation. this characteristic was compatible with the filaroididae family larvae of ...201223082541
spirocerca lupi granulomatous pneumonia in two free-ranging maned wolves (chrysocyon brachyurus) from central brazil.the current report describes granulomatous pneumonia due to spirocerca lupi in 2 free-ranging maned wolves (chrysocyon brachyurus). both wolves had multiple, white, 1-1.5 cm in diameter, soft, encapsulated granulomas in the caudal lung lobes, which contained centrally placed parasites on cut sections. microscopically, there was granulomatous inflammation with numerous intralesional sections of spirurid nematodes. representative complete adult specimens of nematodes derived from these lesions wer ...201425319031
molecular detection of hepatozoon spp. in brazilian and exotic wild carnivores.hepatozoon spp. are apicomplexan parasites that infect a wide variety of animals. the infection occurs through the ingestion of a hematophagous arthropod definitive host. herein, we assessed the presence of hepatozoon spp. in 165 captive wild felids and 100 captive wild canids using molecular techniques. we found that 6 felids (4 little spotted cats, 1 jaguarondi, and 1 puma) and 5 canids (2 bush dogs, 1 fox, 1 crab-eating fox, and 1 maned wolf) were positive for hepatozoon spp. hepatozoon spp. ...201020630658
effectiveness of scat-detection dogs in determining species presence in a tropical savanna landscape.most protected areas are too small to sustain populations of wide-ranging mammals; thus, identification and conservation of high-quality habitat for those animals outside parks is often a high priority, particularly for regions where extensive land conversion is occurring. this is the case in the vicinity of emas national park, a small protected area in the brazilian cerrado. over the last 40 years the native vegetation surrounding the park has been converted to agriculture, but the region still ...201021029162
comparative molecular phylogeny and evolution of sex chromosome dna sequences in the family canidae (mammalia: carnivora).to investigate the molecular phylogeny and evolution of the family canidae, nucleotide sequences of the zinc-finger-protein gene on the y chromosome (zfy, 924-1146 bp) and its homologous gene on the x chromosome (zfx, 834-839 bp) for twelve canid species were determined. the phylogenetic relationships among species reconstructed by the paternal zfy sequences closely agreed with those by mtdna and autosomal dna trees in previous reports, and strongly supported the phylogenetic affinity between th ...201222379982
novel radiographic technique for pregnancy detection in the maned wolf (chrysocyon brachyurus) without anesthesia.maned wolves ( chrysocyon brachyurus ) maintained in ex situ populations challenge veterinarians and managers with high neonatal mortality and parental incompetence. these challenges led to the development of a novel diagnostic approach for pregnancy detection using radiographic imaging without anesthesia or sedation. to do this, a specialized crate was constructed to easily contain a single maned wolf, allowing the capture of lateral projection radiographic images of the abdomen prior to and th ...201728363056
environmental enrichment effect on fecal glucocorticoid metabolites and captive maned wolf (chrysocyon brachyurus) behavior.environmental enrichment is a technique that may reduce the stress of nonhuman animals in captivity. stress may interfere with normal behavioral expression and affect cognitive decision making. noninvasive hormonal studies can provide important information about the stress statuses of animals. this study evaluated the effectiveness of different environmental enrichment treatments in the diminution of fecal glucocorticoid metabolites (stress indicators) of three captive maned wolves (chrysocyon b ...201627057585
gastrointestinal parasites of maned wolf (chrysocyon brachyurus, illiger 1815) in a suburban area in southeastern brazil.we examined 42 maned wolf scats in an unprotected and disturbed area of cerrado in southeastern brazil. we identified six helminth endoparasite taxa, being phylum acantocephala and family trichuridae the most prevalent. the high prevalence of the family ancylostomatidae indicates a possible transmission via domestic dogs, which are abundant in the study area. nevertheless, our results indicate that the endoparasite species found are not different from those observed in protected or least disturb ...201526421763
population structure of mtdna variation due to pleistocene fluctuations in the south american maned wolf (chrysocyon brachyurus, illiger, 1815): management units for conservation.the maned wolf (chrysocyon brachyurus) is one of the largest south american canids, and conservation across this charismatic carnivore's large range is presently hampered by a lack of knowledge about possible natural subdivisions which could influence the population's viability. to elucidate the phylogeographic patterns and demographic history of the species, we used 2 mtdna markers (d-loop and cytochrome b) from 87 individuals collected throughout their range, in argentina, bolivia, brazil, and ...201526245781
induction of ovarian activity and ovulation in an induced ovulator, the maned wolf (chrysocyon brachyurus), using gnrh agonist and recombinant lh.assisted reproductive techniques, such as ovarian manipulation and artificial insemination, are useful for enhancing genetic management of threatened wildlife maintained ex situ. in this study, we used noninvasive fecal hormone monitoring to investigate (1) the influence of pairing with a male on endocrine responses of female maned wolves (chrysocyon brachyurus) to a gnrh agonist (deslorelin) and (2) the efficiency of recombinant lh (relh) on ovulation induction in females housed alone. deslorel ...201424742964
spermatozoa from the maned wolf (chrysocyon brachyurus) display typical canid hyper-sensitivity to osmotic and freezing-induced injury, but respond favorably to dimethyl sulfoxide.we assessed the influences of medium osmolality, cryoprotectant and cooling and warming rate on maned wolf (chrysocyon brachyurus) spermatozoa. ejaculates were exposed to ham's f10 medium (isotonic control) or to this medium plus nacl (350-1000mosm), sucrose (369 and 479mosm), 1m glycerol (1086mosm) or dimethyl sulfoxide (me2so, 1151mosm) for 10 min. each sample then was diluted back into ham's medium and assessed for sperm motility and plasma membrane integrity. although glycerol and me2so had ...201424731851
the complete mitochondrial genome sequence of the maned wolf (chrysocyon brachyurus).in this study, the complete mitochondrial genome of the maned wolf (chrysocyon brachyurus), the unique species in chrysocyon, was sequenced and reported for the first time using blood samples obtained from a female individual in shanghai zoo, china. sequence analysis showed that the genome structure was in accordance with other canidae species and it contained 12 s rrna gene, 16 s rrna gene, 22 trna genes, 13 protein-coding genes and 1 control region.201624708104
the origins of the enigmatic falkland islands wolf.the origins of the extinct falkland islands wolf (fiw), dusicyon australis, have remained a mystery since it was first recorded by europeans in the seventeenth century. it is the only terrestrial mammal on the falkland islands (also known as the malvinas islands), which lie ~460 km from argentina, leading to suggestions of either human-mediated transport or overwater dispersal. previous studies used ancient dna from museum specimens to suggest that the fiw diverged from its closest living relati ...201323462995
adrenal activity in maned wolves is higher on farmlands and park boundaries than within protected areas.in this study we measured excreted fecal corticoid metabolites (fcm) in maned wolves (chrysocyon brachyurus) living within a protected reserve, on farmlands or in a boundary zone between the two habitats, and determined the impacts of season and reproductive status on adrenal activity. feces were collected within a national park (n=191 samples), a park boundary zone (n=39) and on nearby farmlands (n=27), processed and analyzed by enzyme immunoassay. fcm amounts from samples collected on farmland ...201222917914
severe anemia caused by babesiosis in a maned wolf (chrysocyon brachyurus).an 8-yr-old, captive, spayed, female maned wolf (chrysocyon brachyurus) developed progressive lethargy and weakness over a 24-hr period. clinical signs included vomiting, recumbency, horizontal nystagmus, possible blindness, pale icteric mucus membranes, and port-wine colored urine. a complete blood cell count revealed severe anemia (packed cell volume [pcv], 6%) and intraerythrocytic piroplasms consistent with a babesia species. polymerase chain reaction testing later confirmed babesiosis. the ...201222448525
non-invasive sampling in itatiaia national park, brazil: wild mammal parasite detection.non-invasive sampling through faecal collection is one of the most cost-effective alternatives for monitoring of free-living wild mammals, as it provides information on animal taxonomy as well as the dynamics of the gastrointestinal parasites that potentially infect these animals. in this context, this study aimed to perform an epidemiological survey of gastrointestinal parasites using non-invasive faecal samples from carnivores and artiodactyls identified by stool macroscopy, guard hair morphol ...202032807197
osteology and radiology of the maned wolf (chrysocyon brachyurus) pelvic limb.this study describes the osteology and radiology of the pelvic limb in maned wolves. ten (five live and five dead) maned wolves (chrysocyon brachyurus), five males and five females, aged from 2 to 7 years old were used. digital radiographs were taken and recorded for both pelvic limbs in all animals. osteology was correlated with the radiographic images. the pelvis had a rectangular shape, and the obturator foramen (foramen obturatum) was oval. the femoral neck (collum femoris) was short and thi ...201728940671
climate and land-use change will lead to a faunal "savannization" on tropical rainforests.humans have fragmented, reduced or altered the biodiversity in tropical forests around the world. climate and land-use change act synergistically, increasing drought and fire frequencies, converting several tropical rainforests into derived savannas, a phenomenon known as "savannization." yet, we lack a full understanding of the faunal changes in response to the transformation of plant communities. we argue that the composition of vertebrate assemblages in ecotone regions of forest-savanna trans ...202033006792
parental behaviour in maned wolf chrysocyon brachyurus at belo horizonte zoo.there is limited information on the reproductive behaviour of the maned wolf chrysocyon brachyurus. although it is a monogamous species, little is known about the ♂'s role in the rearing of pups. in 1994 a comparative study of the behaviour of two ♀♀ with new-born pups was carried out at belo horizonte zoo to try to establish the ♂'s role in rearing: one ♀ was housed alone with her pups throughout the observation period; the other ♂ was housed with the ♀ and pups for the first month and alone wi ...199732336820
naturally acquired infectious canine hepatitis in two captive maned wolf (chrysocyon brachyurus) puppies.we report the clinicopathological manifestations of canine adenovirus type 1 (cav 1) infection in captive-born naturally infected maned wolves (chrysocyon brachyurus). two 3-month-old maned wolves presented with lethargy, emesis, dehydration, pallor, hypothermia, leucocytosis, neutrophilia, lymphopaenia and thrombocytopaenia. one of the puppies died shortly after admission, with gross changes that included marked gastrointestinal petechiae, splenomegaly, hepatomegaly and pulmonary haemorrhage. h ...202134340805
wild and domestic canids and their interactions in the transmission cycles of trypanosoma cruzi and leishmania spp. in an area of the brazilian cerrado.trypanosoma cruzi and leishmania spp. are parasites that infect multiple hosts including canids, considered bioaccumulators of parasites. deforestation in the cerrado biome has resulted in the exposure of wild canids to anthropized areas, where they may establish ecological and epidemiological relationships with domestic dogs. we evaluated the infection by trypanosomatids in canids from a cerrado agroecosystem between 2013 and 2017. samples of wild canids (blood, bone marrow and skin) and dogs ( ...202033036238
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