Publications

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the use of tadpoles of bufo melanostictus (schneider), rhacophorus leucomystax maculatus (gray) and uperodon sp. in the bioassay of aflatoxins. 19695797383
phylogeography and historical demography of polypedates leucomystax in the islands of indonesia and the philippines: evidence for recent human-mediated range expansion?southeast asia's widespread species offer unique opportunities to explore the effects of geographical barriers to dispersal on patterns of vertebrate lineage diversification. we analyzed mitochondrial gene sequences (16s rdna) from a geographically widespread sample of 266 southeast asian tree frogs, including 244 individuals of polypedates leucomystax and its close relatives. our expectation was that lineages on island archipelagos would exhibit more substantial geographic structure, correspond ...201020601009
rhabdias kongmongthaensis sp. n. (nematoda: rhabdiasidae) from polypedates leucomystax (amphibia: anura: rhacophoridae) in thailand.rhabdias kongmongthaensis sp. n. is described based on specimens found in the lungs of the tree frog polypedates leucomystax (gravenhorst) (amphibia: rhacophoridae) from kanchanaburi province, western thailand. the new species is similar to two north-american species, rhabdias ranae and r. americanus, by presence of two lateral pseudolabia, each with two inner submedian protuberances. r. kongmongthaensis differs from both species by relative length and shape of the tail, and by its distribution ...200516405297
mauthner neurons survive metamorphosis in anurans: a comparative hrp study on the cytoarchitecture of mauthner neurons in amphibians.giant medullary neurons were revealed in adult xenopus laevis and rana esculenta following hrp injections to the spinal cord. these neurons were identified as mauthner neurons because (1) they have the same position and orientation as the larval mauthner neurons, i.e., they lie at the level of the viiith nerve root. (2) they have two large dendritic trees that for each species are similar to those of the larval mauthner neurons, (3) they are clearly distinguishable from other large reticular neu ...19863081602
revision of the frog fly genus caiusa surcouf, 1920 (diptera, calliphoridae), with a note on the identity of plinthomyia emimelania rondani, 1875.the oriental, australasian and oceanian genus caiusa surcouf, 1920 is revised, species concepts being based on male and female genitalia. a key to males for all known species, and a key to females for all except one are given. all relevant types still in existence have been studied, complete synonymies given and the geographical distribution reconsidered. the eight species included in the genus are: caiusa borneoensis sp. nov. (malaysia, thailand, vietnam); caiusa coomani séguy, 1948 (china, mal ...201525947832
unusual mating behavior of the malaysian treefrogs, polypedates leucomystax. 19921574131
genealogy and demographic history of a widespread amphibian throughout indochina.relatively little is known about spatial patterns of cryptic diversity in tropical species and the processes that generate them. few studies examine the geographic distribution of genetic lineages in southeast asia, an area hypothesized to harbor substantial cryptic diversity. we investigated the evolutionary history of asian tree frogs of the polypedates leucomystax complex (n = 172) based on 1800 bp of the mtdna genes nd1 and cytochrome b and tested hypotheses pertaining to climate, geology, a ...201223125408
dna barcoding and the identification of tree frogs (amphibia: anura: rhacophoridae).the dna barcoding gene coi (cytochrome c oxidase subunit i) effectively identifies many species. herein, we barcoded 172 individuals from 37 species belonging to nine genera in rhacophoridae to test if the gene serves equally well to identify species of tree frogs. phenetic neighbor joining and phylogenetic bayesian inference were used to construct phylogenetic trees, which resolved all nine genera as monophyletic taxa except for rhacophorus, two new matrilines for liuixalus, and polypedates leu ...201626004249
zygocaulus nagoensis n. gen. and n. sp. (trichostrongyloidea: dictyocaulidae), a peculiar bursate nematode collected from an alien frog, polypedates leucomystax, in nago, okinawa island, japan.a peculiar bursate nematode, zygocaulus nagoensis n. gen. and n. sp. (trichostrongyloidea: dictyocaulidae: mertensinematinae), was described from an alien frog, polypedates leucomystax (anura: rhacophoridae), collected on okinawa island, japan. it is related to mertensinema and borrellostrongylus, the only hitherto known genera of mertensinematinae, but is readily distinguished from them by having only 2 pairs of lateral rays and simple distal ends of the dorsal ray branches.201931116078
crystallization of ranasmurfin, a blue-coloured protein from polypedates leucomystax.ranasmurfin, a previously uncharacterized approximately 13 kda blue protein found in the nests of the frog polypedates leucomystax, has been purified and crystallized. the crystals are an intense blue colour and diffract to 1.51 a with p2(1) symmetry and unit-cell parameters a = 40.9, b = 59.9, c = 45.0 a, beta = 93.3 degrees . self-rotation function analysis indicates the presence of a dimer in the asymmetric unit. biochemical data suggest that the blue colour of the protein is related to dimer ...200617077494
phylogenetic relationships and genetic diversity of the polypedates leucomystax complex in thailand.taxonomic uncertainty of the asian tree frog polypedates leucomystax complex presents the challenging task of inferring its biogeographical history. here, we describe its dispersion and the genetic relationships among different populations in thailand, where we connect the population of the p. leucomystax complex of the sunda islands to the indochina (mainland) population based on analyses of 266 sequences of the mitochondrial cytochrome c oxidase subunit i (coi) gene. our maternal genealogy imp ...201829362696
ultrastructure and motility of the spermatozoa of polypedates leucomystax (amphibia, anura, rhacophoridae).sperm morphology is thought to be shaped by evolutionary pressure from the fertilization environment. rhacophoridae (amphibia, anura) include both foam-nesting and nonfoam-nesting species and exhibit a variety of sperm morphologies. here, we examine the sperm morphology and motility of a foam-nesting rhacophoridae frog, polypedates leucomystax. their spermatozoa have a sickle-shaped head and a thick tail containing two axonemes with their doublet microtubule ones (db1s) facing one another. these ...201323125050
a new tree frog in the genus polypedates (anura: rhacophoridae) from southern thailand.we describe a new species of southeast asian rhacophorid frog belonging to the polypedates leucomystax species complex from songkhla province, southern thailand. polypedates discantus sp. nov. is distinguished from its congeners by the combination of having the skin of the head not co-ossified with the skull; absent or indistinct white dots on the back of the thigh; paired-vocal sac openings; and a round tubercle on the tibiotarsal articulation. the new species is also distinguished from p. leuc ...201326146745
protozoan parasites of four species of wild anurans from a local zoo in malaysia.the parasitic protozoan fauna in sixty-six anurans comprising of duttaphrynus melanostictus, phrynoidis juxtaspera, hylarana erythraea and polypedates leucomystax collected from zoo negara malaysia was investigated. the distribution and prevalence rate of parasitic species in the digestive tract and blood were examined. seven species of intestinal protozoa (opalina ranarum, cepedea dimidiata, nycthetorus cordiformis, entamoeba ranarum, iodamoeba butschlii, endamoeba blattae, and tritrichomonas s ...201324522131
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