| biochemical identification of citrobacter species defined by dna hybridization and description of citrobacter gillenii sp. nov. (formerly citrobacter genomospecies 10) and citrobacter murliniae sp. nov. (formerly citrobacter genomospecies 11). | recent work describing six named species and two unnamed genomospecies within citrobacter has enlarged the genus to 11 species. dna relatedness and phenotypic tests were used to determine how well these species can be identified. one hundred thirty-six strains were identified to species level by dna relatedness and then identified phenotypically in a blinded fashion. by using conventional tests, 119 of the 136 strains (88%) were correctly identified to species level. three additional strains (2% ... | 1999 | 10405411 |
| structure of the o-specific polysaccharide isolated from the lipopolysaccharide of citrobacter gillenii serotype o12a, 12b strain pcm 1544. | a neutral o-specific polysaccharide was isolated from the lipopolysaccharide of citrobacter gillenii strain pcm 1544, representing serotype o12a,12b. the polysaccharide was studied by sugar and methylation analyses and smith degradation along with 1h and 13c nmr spectroscopy, including a roesy experiment. the following structure of the tetrasaccharide repeating unit was established, in which substitution with terminal glcnac is approximately 60%. [structure: see text] | 2001 | 11383903 |
| structures of two o-chain polysaccharides of citrobacter gillenii o9a,9b lipopolysaccharide. a new homopolymer of 4-amino-4,6-dideoxy-d-mannose (perosamine). | mild acid degradation of the lipopolysaccharide of citro- bacter gillenii o9a,9b released a polysaccharide (ps), which was found to consist of a single monosaccharide, 4- acetamido-4,6-dideoxy-d-mannose (d-rha4nac, n-acetyl-d-perosamine). ps was studied by methylation analysis and (1)h-nmr and (13)c-nmr spectroscopy, using two-dimensional (1)h,(1)h cosy, tocsy, noesy, and h-detected (1)h,(13)c heteronuclear correlation experiments. it was found that ps includes two structurally different polysac ... | 2002 | 11784302 |
| structure of a new 2-deoxy-2-[(r)-3-hydroxybutyramido]-d-glucose-containing o-specific polysaccharide from the lipopolysaccharide of citrobacter gillenii pcm 1542. | the o-specific polysaccharide of citrobacter gillenii pcm 1542 from serotype o-12a,12 b is composed of one residue each of d-glucose, d-glcnac, 2-deoxy-2-[(r)-3-hydroxybutyramido]-d-glucose (d-glcnacyl) and two galnac residues. on the basis of sugar and methylation analyses of the intact and smith degraded polysaccharides, along with 1d and 2d 1h and 13c nmr spectroscopy, the following structure of the branched pentasaccharide repeating unit of the o-specific polysaccharide was established:this ... | 2002 | 12350323 |
| structure of the o-specific polysaccharide from the lipopolysaccharide of citrobacter gillenii o11, strain pcm 1540. | the o-specific polysaccharide of the lipopolysaccharide of citrobacter gillenii pcm 1540 (serogroup o11) consists of d-glc, d-man, d-galnac, d-glcnac, 2-acetamido-2,6-dideoxy-d-galactose (d-fucnac) and o-acetyl groups in the ratios 2:1:1:1:1:1. on the basis of sugar and methylation analyses and smith-degradation along with 1d and 2d 1h and 13c nmr spectroscopy, the following structure of the branched hexasaccharide repeating unit was established: [structure: see text]. citrobacter werkmanii pcm ... | 2003 | 12801712 |
| chromosome-encoded narrow-spectrum ambler class a beta-lactamase gil-1 from citrobacter gillenii. | a novel beta-lactamase gene was cloned from the whole-cell dna of an enterobacterial citrobacter gillenii reference strain that displayed a weak narrow-spectrum beta-lactam-resistant phenotype and was expressed in escherichia coli. it encoded a clavulanic acid-inhibited ambler class a beta-lactamase, gil-1, with a pi value of 7.5 and a molecular mass of ca. 29 kda. gil-1 had the highest percent amino acid sequence identity with tem-1 and shv-1, 77%, and 67%, respectively, and only 46%, 31%, and ... | 2007 | 17242148 |
| synthesis of spacer-equipped di-, tri-, and the tetrasaccharide fragments of the deacetylated o-ps1 of citrobacter gillenii o9a,9b, and a related pentasaccharide. | the title rhamnooligosaccharides [alpha-d-rhap4nac-(1-->3)-alpha-d-rhap4nac-1-o-(ch(2))(5)coome, alpha-d-rhap4nac-(1-->3)-alpha-d-rhap4nac-(1-->3)-alpha-d-rhap4nac-1-o-(ch(2))(5)coome, alpha-d-rhap4nac-(1-->2)-alpha-d-rhap4nac-(1-->3)-alpha-d-rhap4nac-(1-->3)-alpha-d-rhap4nac-1-o-(ch(2))(5)coome, and alpha-d-rhap4nac-(1-->3)-alpha-d-rhap4nac-(1-->2)-alpha-d-rhap4nac-(1-->3)-alpha-d-rhap4nac-(1-->3)-alpha-d-rhap4nac-1-o-(ch(2))(5)coome] were synthesized in a stepwise fashion from 5-methoxycarbony ... | 2008 | 18436197 |
| study of the bacterial ecosystem in tropical cooked and peeled shrimps using a polyphasic approach. | the characterization of the microbial ecosystem of cooked tropical shrimps was carried out using a polyphasic approach. first, culture-dependent methods were used for bacterial enumeration and the phenotypic and molecular identification of bacterial isolates. then, culture-independent methods, including pcr-ttge (v3 region of the 16s rrna gene), provided a fingerprinting of bacterial dna directly extracted from shrimps. two batches of cooked and peeled tropical shrimps were stored at 5 and 15 de ... | 2009 | 18573557 |
| molecular analysis of intestinal microbiota of rainbow trout (oncorhynchus mykiss). | the aim of this study was to evaluate different molecular tools based on the 16s rrna gene, internal transcribed spacer, and the rpob gene to examine the bacterial populations present in juvenile rainbow trout intestines. dna was extracted from both pooled intestinal samples and bacterial strains. genes were pcr-amplified and analysed using both temporal temperature gradient gel electrophoresis (ttge) and restriction fragment length polymorphism methods. because of the high cultivability of the ... | 2010 | 19780831 |
| molecular characterization and antimicrobial resistance profile of atypical citrobacter gillenii and citrobacter sp. isolated from diseased rainbow trout (oncorhynchus mykiss). | over the last decade, citrobacter species have been responsible for infections in fish and many species and also new citrobacter species have been identified. in this study, molecular identifications and the phenotypic and genotypic antimicrobial-resistance characteristics of atypical and typical citrobacter species were determined. | 2017 | 28732787 |