molecular evolution of a host-range gene in geminiviruses infecting asexual populations of eupatorium makinoi. | asexual plants of eupatorium makinoi is frequently infected with tobacco leaf curl geminivirus (tlcv). the host range of tlcv is narrow, and orf c4 is considered to function as a host range determinant. using this tlcv-eupatorium system, we tested the expectation that the rate of amino acid replacements will be accelerated in orf c4 if resistant genes of the host plants drive molecular evolution in orf c4. orf c4 is entirely contained within a longer orf c1 encoding a replication protein. we ana ... | 1998 | 9794079 |
characterization of tobacco geminiviruses in the old and new world. | biological differences and molecular variability between six phenotypically distinct tobacco-infecting geminivirus isolates from southern africa (zimbabwe) and mexico were investigated. host range studies conducted with tobacco virus isolates zim h from zimbabwe and mex 15 and mex 32 from mexico indicated all had narrow host ranges restricted to the solanaceae. alignment of coat protein gene (cp) and common region (cr) sequences obtained by pcr, and phylogenetic analysis of the cp sequences indi ... | 1999 | 10365162 |
complete nucleotide sequence and the genome organization of tobacco leaf curl geminivirus from japan. | the genomic dna of tobacco leaf curl geminivirus (tlcv) from tomato plants with leaf curl disease in japan has been sequenced. the single circular dna molecule comprises 2,761 nucleotides. tlcv dna contains six open reading frames (orfs) capable of encoding proteins with a molecular weight greater than 10 k. in total nucleotide sequence comparisons with other geminiviruses, tlcv was most closely related to tomato leaf curl virus from taiwan (twtolcv) (76% identity), tomato leaf curl virus from b ... | 1999 | 10585147 |
molecular characterization of a novel defective dna isolated from tobacco tissues infected with tobacco leaf curl virus. | defective dna of tobacco leaf curl virus (tlcv) was identified in tlcv-infected tobacco plants. the defective dna was cloned and sequenced. the sequence showed it was about half the size of the tlcv dna-a, and was derived from tlcv dna-a by a large deletion. the defective dna contained the intergenic region and part of the ac1 (rep) gene of tlcv, and also novel open reading frames (orfs). the immunotrapping tests showed the defective dna was associated with geminate particles, suggesting it coul ... | 2001 | 11394577 |
molecular characterization of a distinct begomovirus infecting tobacco in yunnan, china. | virus isolate y3v, obtained from tobacco showing leaf curl symptoms in yunnan, china, had particles with the size and morphology typical of geminiviruses. in reactions with a set of monoclonal antibodies raised against begomoviruses, y3v was readily differentiated from two previously studied chinese begomovirus isolates. the complete nucleotide sequence of a dna-a-like molecule of y3v was determined; it comprises 2744 nucleotides and has a typical begomovirus genome organization. when compared w ... | 2001 | 11676420 |
evidence for recombination among isolates of tobacco leaf curl japan virus and honeysuckle yellow vein mosaic virus. | complete nucleotide sequences of tobacco leaf curl japan virus (tblcjv) isolates from infected tomato ( lycopersicon esculentum) plants in nara (-[jp2], 2764 nt; -[jp3], 2761 nt), kochi (-[koc], 2760 nt) and yamaguchi (-[yam], 2758 nt) prefectures, of japan were determined. these sequences were compared with each other and the sequences of further begomoviruses from japan. tblcjv, tblcjv-[jp2], tblcjv-[jp3], tblcjv-[koc], tblcjv-[yam], honeysuckle yellow vein mosaic virus (hyvmv), eupatorium yel ... | 2004 | 15168208 |
chinese tomato yeliow ieaf curl virus- a new species of geminivirus. | chine tomato yellow leaf curl virus (tylcv-chi) and other geminiviruses were analysed with 20 monoclonai antibodies. it was shown that tylcv-chi is serclogicaily close to chinese tabacco ieaf curl virus (tblcv-chi). the fragment of tylcv-chi dna including the common region (cr), n-terminal of coat protein gene and av1 gene was amplified by pcr and cloned, and its dna sequence was determined. these raults showed that tylcv-chi is different from other known geminiviruses in the world, and is a new ... | 1998 | 18726248 |
phylogenetic lineage of tobacco leaf curl virus in korea and estimation of recombination events implicated in their sequence variation. | new strains of tobacco leaf curl virus (tblcv) were isolated from tomato plants in four different local communities of korea, and hence were designated tblcv-kr. phylogenetic analysis of the sequences of the whole genome and of individual orfs of these viruses indicated that they are closely related to the tobacco leaf curl japan virus (tblcjv) cluster, which includes honeysuckle yellow vein virus (hyvv), honeysuckle yellow vein mosaic virus (hyvmv), and tblcjv isolates. four putative recombinat ... | 2011 | 21549772 |
host range and genetic diversity of croton yellow vein mosaic virus, a weed-infecting monopartite begomovirus causing leaf curl disease in tomato. | croton yellow vein mosaic virus (cyvmv) is a widely occurring begomovirus in croton bonplandianum, a common weed in the indian subcontinent. in this study, cyvmv (genus begomovirus, family geminiviridae) was transmitted by whiteflies (bemisia tabaci) to as many as 35 plant species belonging to 11 families, including many vegetables, tobacco varieties, ornamentals and weeds. cyvmv produced bright yellow vein symptoms in croton, whereas in all the other host species, the virus produced leaf curl s ... | 2013 | 23096697 |
effects of virus infection on demographic traits of an agamospermous population of eupatorium chinense (asteraceae). | there are few studies of the interaction between wild plants and viruses. in this paper, the incidence of a geminivirus (tobacco leaf curl virus, tlcv) infection, and its effects on mortality, growth and reproduction of its host-plant, eupatorium chinense, are reported. a total of 221 plants of an agamospermous population of e. chinense were chosen and their demographic behaviour followed over 2 years (1991-1992). the proportion of infected plants differed between years, with fewer plants infect ... | 1993 | 28313644 |