| the genome organization of banana bunchy top virus: analysis of six ssdna components. | we have cloned, sequenced and analysed an additional five circular ssdna components of banana bunchy top virus (bbtv) which we have called components 2, 3, 4, 5 and 6. these components were present in all bbtv infections tested. four of these components (components 3, 4, 5 and 6) had one large open reading frame (orf) in the virion sense located 3' of a stem-loop structure. each orf had a potential tata box and one or two potential polyadenylation signals associated with it and each polyadenylat ... | 1995 | 7782775 |
| ten distinct circular ssdna components, four of which encode putative replication-associated proteins, are associated with the faba bean necrotic yellows virus genome. | four further circular ssdna components (c7-c10), about 1 kb in size and structurally similar to the previously described components (c1-c6) found associated with a syrian (sy) isolate of faba bean necrotic yellows virus (fbnyv), have been identified. similar to c1 and c2, two of the new components (c7 and c9) encode putative replication-associated (rep) proteins of 33.2 and 32.7 kda, respectively, the former of which is 90% identical to the c10 rep protein of milk vetch dwarf virus (mdv). c8 enc ... | 1998 | 9880028 |
| sequences of ten circular ssdna components associated with the milk vetch dwarf virus genome. | milk vetch dwarf virus (mdv) is a member of the proposed genus nanovirus, and its genome is composed of multiple, circular ssdna components of about 1 kb. we have cloned and sequenced ten ssdna components and designated them mdv-c1 to c10. each dna component contains one potential major open reading frame, and contains a putative stem-loop structure in the non-coding region. notably, four components (c1, c2, c3 and c10) encode distinct replication-associated (rep) proteins of 33 kda, which show ... | 1998 | 9880029 |
| the master rep concept in nanovirus replication: identification of missing genome components and potential for natural genetic reassortment. | faba bean necrotic yellows virus (fbynv), milk vetch dwarf virus (mdv), and subterranean clover stunt virus (scsv) are nanoviruses that infect leguminous plants. from mdv- and scsv-infected tissue we identified viral dnas that encode a replication initiator protein (rep), essential for replication of the multiple circular single-stranded dnas of these viruses. these previously undescribed rep proteins of mdv and scsv are strikingly similar in sequence and functionally equivalent to the master re ... | 2000 | 10936099 |
| the promoter of milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants. | the activity of a predicted promoter, pmc8, from milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35s rna promoter (p35s) and pncr, a promoter from soybean chlorotic mottle virus. when the gus fusion gene was introduced into tobacco, pmc8 showed a similar expression profile to p35s but with a more intense expression in proliferating tissues. the usefulness of pmc8 was confirmed by driving nptii for selection of kanamycin-resistant tobacco plants with improve ... | 2005 | 15812660 |
| characteristics of the promoters derived from the single-stranded dna components of milk vetch dwarf virus in transgenic tobacco. | predicted promoter regions of milk vetch dwarf virus (mdv) components (c1-c11) were isolated and fused with a beta-glucuronidase (gus) reporter gene and the characteristics of the promoters were examined. in transgenic tobacco calli, promoters of mdv c4 (encoding a cell-cycle link protein), c5 and c7 (both encoding unknown proteins), c6 (encoding a nuclear-shuttle protein) and c8 (encoding a movement protein) generated a stronger level of gus expression than the cauliflower mosaic virus 35s rna ... | 2005 | 15914865 |
| two distinct nanovirus species infecting faba bean in morocco. | using monoclonal antibodies raised against a faba bean necrotic yellows virus (fbnyv) isolate from egypt and a faba bean necrotic stunt virus (fbnsv) isolate from ethiopia, a striking serological variability among nanovirus isolates from faba bean in morocco was revealed. to obtain a better understanding of this nanovirus variability in morocco, the entire genomes of two serologically contrasting isolates referred to as mor5 and mor23 were sequenced. the eight circular ssdna components, each ide ... | 2010 | 20069400 |
| complete genomic characterization of milk vetch dwarf virus isolates from cowpea and broad bean in anhui province, china. | cowpea and broad bean plants showing severe stunting and leaf rolling symptoms were observed in hefei city, anhui province, china, in 2014. symptomatic plants from both species were shown to be infected with milk vetch dwarf virus (mdv) by pcr. the complete genomes of mdv isolates from cowpea and broad bean were sequenced. each of them had eight genomic dnas that differed between the two isolates by 10.7% in their overall nucleotide sequences. in addition, the mdv genomes from cowpea and broad b ... | 2017 | 28386650 |