| psychromonas antarcticus gen. nov., sp. nov., a new aerotolerant anaerobic, halophilic psychrophile isolated from pond sediment of the mcmurdo ice shelf, antarctica | a gram-negative, rod- to oval-shaped, aerotolerant anaerobic bacterium was isolated from an anaerobic enrichment inoculated with sediment taken from below the cyanobacterial mat of a high-salinity pond near bratina island on the mcmurdo ice shelf, antarctica. the organism was positive for terminal oxidase and catalase and was motile by means of a polar flagellum. optimal growth of anaerobic cultures occurred at 12 degrees c, at ph 6.5, and at an nacl concentration of 3% (w/v). of a variety of po ... | 1998 | 9477258 |
| psychromonas marina sp. nov., a novel halophilic, facultatively psychrophilic bacterium isolated from the coast of the okhotsk sea. | a facultatively psychrophilic bacterium, strain 4-22t, was isolated from a cold current off the monbetsu coast of the okhotsk sea in hokkaido, japan. the isolate was a rod-shaped facultative anaerobe that reduced nitrate to nitrite and hydrolysed starch, dna and alginic acid, but not chitin or gelatin. the isolate grew at 0 degrees c, but not at 26 degrees c; the optimum growth temperature was 14-16 degrees c. nacl was required for growth. the dna g+c content was 43.5 mol%. the whole-cell fatty ... | 2002 | 12361246 |
| psychromonas kaikoae sp. nov., a novel from the deepest piezophilic bacterium cold-seep sediments in the japan trench. | two strains of obligately piezophilic bacteria were isolated from sediment collected from the deepest cold-seep environment with chemosynthesis-based animal communities within the japan trench, at a depth of 7434 m. the isolated strains, jt7301 and jt7304t, were closely affiliated with members of the genus psychromonas on the basis of 16s rdna sequence analysis. hybridization values for dna-dna relatedness between these strains and the psychromonas antarctica reference strain were significantly ... | 2002 | 12361254 |
| psychromonas profunda sp. nov., a psychropiezophilic bacterium from deep atlantic sediments. | a psychropiezophilic bacterium, strain 2825t (=lmg 21260t =jcm 11437t), isolated from deep atlantic sediments at a depth of 2770 m and a temperature of 2 degrees c, was found by polyphasic analysis to represent a novel species of the genus psychromonas, psychromonas profunda sp. nov. it is a strict psychrophile and a moderate piezophile, whose degree of piezophily is increased markedly when the temperature is raised to 10 degrees c. the piezophily of p. profunda is intermediate between that of t ... | 2003 | 12710622 |
| psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from spitzbergen. | using starch as a carbon source at a cultivation temperature of 4 degrees c, a number of gram-negative, aerobic strains was isolated from sea-ice and sea-water samples collected at spitzbergen in the arctic. analysis of the genetic diversity of the novel isolates by random amplification of polymorphic dna (rapd) and eric fingerprinting revealed a homogenic group of biofilm-forming bacteria that contained small extrachromosomal elements. as a representative of the group, strain pull 5.3t, isolate ... | 2003 | 12710624 |
| subfreezing growth of the sea ice bacterium "psychromonas ingrahamii". | "psychromonas ingrahamii," named for john l. ingraham, was isolated from sea ice from off point barrow, alaska. this large rod-shaped bacterium belongs to the gamma-proteobacteria. "p. ingrahamii" is a psychrophilic, heterotrophic bacterium that is gas vacuolate and nonmotile. "p. ingrahamii" is notable in that it grows at a temperature of -12 degrees c with a generation time of 240 h. this is the lowest growth temperature of any organism authenticated by a growth curve. | 2004 | 14994177 |
| phylogenetic relationships among marine alteromonas-like proteobacteria: emended description of the family alteromonadaceae and proposal of pseudoalteromonadaceae fam. nov., colwelliaceae fam. nov., shewanellaceae fam. nov., moritellaceae fam. nov., ferrimonadaceae fam. nov., idiomarinaceae fam. nov. and psychromonadaceae fam. nov. | the phylogenetic relationships among marine alteromonas-like bacteria of the genera alteromonas, pseudoalteromonas, glaciecola, thalassomonas, colwellia, idiomarina, oceanimonas, oceanisphaera, shewanella, moritella, ferrimonas, psychromonas and several other genera of the 'gammaproteobacteria' were studied. results of 16s rrna gene sequence analyses revealed that some members of these genera formed several coherent groups at the family level. characteristic signature oligonucleotides for studie ... | 2004 | 15388743 |
| psychromonas ingrahamii sp. nov., a novel gas vacuolate, psychrophilic bacterium isolated from arctic polar sea ice. | a gas vacuolate bacterium, designated strain 37t, was isolated from a sea ice core collected from point barrow, alaska, usa. cells of strain 37t were large (6-14 microm in length), rod-shaped, contained gas vacuoles of two distinct morphologies, and grew well at nacl concentrations of 1-10 % and at temperatures of -12 to 10 degrees c. the dna g+c content was 40 mol%. whole-cell fatty acid analysis showed that 16 : 1omega7c comprised 67 % of the total fatty acid content. phylogenetic analysis of ... | 2006 | 16627645 |
| marine bacteria associated with the korean brown alga, undaria pinnatifida. | several marine bacterial strains were isolated from undaria pinnatifida (miyok in korean). sixty-six strains were isolated on r2a agar media at 10 degrees and identified by a phylogenetic analysis of the 16s rrna gene sequences. they were grouped into 10 different sequence types based on the initial sequence analysis of the 5' domain of the gene (approximately 500 bp). full sequences of 16s rrna gene were obtained from one strain in each sequence type and the species-affiliation was determined u ... | 2006 | 17205052 |
| psychromonas hadalis sp. nov., a novel piezophilic bacterium isolated from the bottom of the japan trench. | an obligately piezophilic bacterium was isolated from sediment collected from the bottom of the japan trench at a depth of 7542 m. the isolated strain, designated k41g(t), was closely affiliated with members of the genus psychromonas on the basis of 16s rrna gene sequence analysis. levels of dna-dna relatedness between strain k41g(t) and psychromonas reference strains were significantly lower than that accepted as the phylogenetic definition of a species. the optimal temperature and pressure for ... | 2007 | 17551059 |
| genomics of an extreme psychrophile, psychromonas ingrahamii. | the genome sequence of the sea-ice bacterium psychromonas ingrahamii 37, which grows exponentially at -12c, may reveal features that help to explain how this extreme psychrophile is able to grow at such low temperatures. determination of the whole genome sequence allows comparison with genes of other psychrophiles and mesophiles. | 2008 | 18460197 |
| psychromonas japonica sp. nov., psychromonas aquimarina sp. nov., psychromonas macrocephali sp. nov. and psychromonas ossibalaenae sp. nov., psychrotrophic bacteria isolated from sediment adjacent to sperm whale carcasses off kagoshima, japan. | four novel species of psychrotrophic bacteria were isolated from sediment adjacent to sperm whale carcasses off kagoshima, japan, at a depth of 228-250 m. cells of the five isolated strains, jamm 0394(t), jamm 0404(t), jamm 0415(t), jamm 0700 and jamm 0738(t), were gram-negative, rod-shaped, non-sporulating and motile by means of a single polar flagellum. the novel strains were able to produce isoprenoid quinone q-8 as the major component. the predominant fatty acids were c(16 : 0) and c(16 : 1) ... | 2008 | 18599721 |
| highly phosphorylated core oligosaccharide structures from cold-adapted psychromonas arctica. | many cold habitats contain plenty of microorganisms that represent the most abundant cold-adapted life forms on earth. these organisms have developed a wide range of adaptations that involve the cell wall of the microorganism. in particular, bacteria enhance the synthesis of unsaturated fatty acids of membrane lipids to maintain the membrane fluidity, but very little is known about the adaptational changes in the structure of the lipopolysaccharides (lpss), the main constituent of the outer leaf ... | 2008 | 18770509 |
| psychromonas heitensis sp. nov., a psychrotolerant bacterium isolated from seawater in japan. | four bacterial strains, 04he-4-40, a4i-9, a4i-21 and ak15-027(t), were isolated from heita bay off kamaishi in japan. based on 16s rrna gene sequence analysis, the isolates were placed as a new lineage within the genus psychromonas. the 16s rrna gene sequence similarity values between the isolates and type strains of recognized psychromonas species were less than 97 %. the dna g+c contents of the isolates were about 38 mol%. the major respiratory quinone was q-8, and the predominant cellular fat ... | 2008 | 18842836 |
| identification and expression of the tig gene coding for trigger factor from psychrophilic bacteria with no information of genome sequence available. | trigger factor (tf) plays a key role as a molecular chaperone with a peptidyl-prolyl cis-trans isomerase (ppiase) activity by which cells promote folding of newly synthesized proteins coming out of ribosomes. since psychrophilic bacteria grow at a quite low temperature, between 4 and 15 degrees c, tf from such bacteria was investigated and compared with that of mesophilic bacteria e. coli in order to offer an explanation of cold-adaptation at a molecular level. using a combination of gradient pc ... | 2009 | 19459006 |
| psychromonas agarivorans sp. nov., a novel agarolytic bacterium. | two heterotrophic, agarolytic bacteria were isolated from marine environments. a 16s rrna gene sequence analysis showed the isolates (designated strains j42-3a(t) and 04oz-as15-7a) to be positioned in a separate lineage within the genus psychromonas. members of the genus psychromonas are recognized as being psychrophilic or psychrotolerant, whereas none of the currently established psychromonas species is known to be agarolytic. the dna g+c contents of the isolates were about 42 mol% and the pre ... | 2009 | 19502298 |
| psychromonas boydii sp. nov., a gas-vacuolate, psychrophilic bacterium isolated from an arctic sea-ice core. | a gas-vacuolate bacterium, strain 174(t), was isolated from a sea-ice core collected from point barrow, alaska, usa. comparative analysis of 16s rrna gene sequences showed that this bacterium was most closely related to psychromonas ingrahamii 37(t), with a similarity of >99 %. however, strain 174(t) could be clearly distinguished from closely related species by dna-dna hybridization; relatedness values determined by two different methods between strain 174(t) and p. ingrahamii 37(t) were 58.4 a ... | 2010 | 19648341 |
| structural adaptation of serine hydroxymethyltransferase to low temperatures. | structural adaptation of serine hydroxymethyltransferase (shmt), a pyridoxal-5'-phosphate dependent enzyme that catalyzes the reversible conversion of l-serine and tetrahydropteroylglutamate to glycine and 5,10-methylene-tetrahydropteroylglutamate, synthesized by microorganisms adapted to low temperatures has been analyzed using a comparative approach. the variations of amino acid properties and frequencies among three temperature populations (psychrophilic, mesophilic, hyper- and thermophilic) ... | 2010 | 19815026 |
| overexpression of cold shock protein a of psychromonas arctica kopri 22215 confers cold-resistance. | a polar bacterium was isolated from arctic sea sediments and identified as psychromonas artica, based on 16s rdna sequence. psychromonas artica kopri 22215 has an optimal growth temperature of 10 degrees c and a maximum growth temperature of 25 degrees c, suggesting this bacterium is a psychrophile. cold shock proteins (csps) are induced upon temperature downshift by more than 10 degrees c. functional studies have researched mostly csps of a mesophilic bacterium escherichia coli, but not on thos ... | 2010 | 20169403 |
| identifying fermenting bacteria in anoxic tidal-flat sediments by a combination of microcalorimetry and ribosome-based stable-isotope probing. | a novel approach was developed to follow the successive utilization of organic carbon under anoxic conditions by microcalorimetry, chemical analyses of fermentation products and stable-isotope probing (sip). the fermentation of (13) c-labeled glucose was monitored over 4 weeks by microcalorimetry in a stimulation experiment with tidal-flat sediments. based on characteristic heat production phases, time points were selected for quantifying fermentation products and identifying substrate-assimilat ... | 2011 | 22188432 |
| marine bacterioplankton diversity and community composition in an antarctic coastal environment. | the bacterial community inhabiting the water column at terra nova bay (ross sea, antarctica) was examined by the fluorescent in situ hybridization (fish) technique and the genotypic and phenotypic characterization of 606 bacterial isolates. overall, the fish analysis revealed a bacterioplankton composition that was typical of antarctic marine environments with the cytophaga/flavobacter (cf) group of bacteroidetes that was equally dominant with the actinobacteria and gammaproteobacteria. as sampl ... | 2011 | 21748267 |