| five new species of henneguya (protozoa: myxosporida) from ictalurid fishes. | four new species of henneguya (myxosporidan) are described from ictalurus punctatus rafinesque (channel catfish). they are as follows: henneguya postexilis sp. n. and henneguya longicauda sp. n. from the gills; henneguya adiposa sp. n. from the adipose fin; and henneguya diversis sp. n. from the liver, kidney, connective tissue of muscles and fins, and tumor-like external growths. henneguya pellis sp. n. is described from the dermis of ictalurus furcatus (lesueur) (blue catfish). the development ... | 1977 | 881651 |
| absence of ascorbic acid synthesis in channel catfish, ictalurus punctatus and blue catfish, ictalurus frucatus. | | 1973 | 4754775 |
| fish tissue quality in the lower mississippi river and health risks from fish consumption. | between 1990 and 1994, samples of three shellfish species (i.e. blue crab, callinectes sapidus;crayfish, procambarus acutis; and river shrimp, macrobrachium ohionii) and 16 fish species and were collected at six sites along the lower mississippi river by the louisiana department of environmental quality, office of water resources in coordination with the us environmental protection agency. the fish species included: bigmouth buffalo (ictiobus cyanellus); blue catfish (ictalurus furcatus); carp ( ... | 2003 | 12526903 |
| catfish hepcidin gene is expressed in a wide range of tissues and exhibits tissue-specific upregulation after bacterial infection. | antimicrobial peptides (amps) are important components of the host innate immune response against microbial invasion. the cysteine-rich amps such as defensin and hepcidin have been extensively studied from various organisms, but their role in disease defense in catfish is unknown. as a first step, we sequenced a hepcidin cdna from both channel catfish and blue catfish, and characterized the channel catfish hepcidin gene. the channel catfish hepcidin gene consists of two introns and three exons t ... | 2005 | 15935472 |
| a broadly applicable method to characterize large dna viruses and adenoviruses based on the dna polymerase gene. | many viral pathogens are poorly characterized, are difficult to culture or reagents are lacking for confirmatory diagnoses. we have developed and tested a robust assay for detecting and characterizing large dna viruses and adenoviruses. the assay is based on the use of degenerate pcr to target a gene common to these viruses, the dna polymerase, and sequencing the products. | 2006 | 16608526 |
| comparative study of mannose-binding c-type lectin isolated from channel catfish (ictalurus punctatus) and blue catfish (ictalurus furcatus). | mannose-binding c-type lectin (mbl) was isolated from channel catfish (ictalurus punctatus) nwac 102 and 103 strains, blue catfish (ictalurus furcatus) d+b and rio grande strains, hybrid catfish (channel catfish female nwac 103 x blue catfish male d+b) sera, and purified by affinity chromatography from channel catfish norris strain serum. reduction of purified channel catfish mbl with 2-me yielded a single band of 62 kda by sds-page and western blot analysis using guinea pig anti-mbl igg as prim ... | 2007 | 17950622 |
| the two channel catfish intelectin genes exhibit highly differential patterns of tissue expression and regulation after infection with edwardsiella ictaluri. | intelectins (intl) are ca(2+)-dependent secretory glycoproteins that play a role in the innate immune response. the mammalian intl is also known as lactoferrin receptor (lfr) that is involved in iron metabolism. the objective of this study was to characterize the intelectin genes in both channel catfish and blue catfish, to determine their genomic organization and copy numbers, to determine their patterns of tissue expression, and to establish if they are involved in defense responses of catfish ... | 2008 | 18078992 |
| effect of preservatives on microbial safety and quality of smoked blue catfish (ictalurus furcatus) steaks during room-temperature storage. | the microbial safety and quality of smoked blue catfish (ictalurus furcatus) steaks treated with antimicrobials and antioxidants were examined during 6-week ambient storage. five pre-smoking soaking treatments were applied: 25% nacl and 1% ascorbic acid for 30 min or 1h, 3% sodium lactate with or without 5% rosemary extract for 30 min, and 5% sorbic acid alone for 30 min. after smoking, cooled catfish steaks were packed and analyzed at 0, 2, 4, and 6 weeks of ambient storage. neither listeria no ... | 2008 | 18954730 |
| a genome-wide association study in catfish reveals the presence of functional hubs of related genes within qtls for columnaris disease resistance. | columnaris causes severe mortalities among many different wild and cultured freshwater fish species, but understanding of host resistance is lacking. catfish, the primary aquaculture species in the united states, serves as a great model for the analysis of host resistance against columnaris disease. channel catfish in general is highly resistant to the disease while blue catfish is highly susceptible. f2 generation of hybrids can be produced where phenotypes and genotypes are segregating, provid ... | 2015 | 25888203 |
| new data on henneguya pellis (myxozoa: myxobolidae), a parasite of blue catfish ictalurus furcatus. | the original description of henneguya pellis, a myxozoan parasitizing blue catfish ictalurus furcatus, is supplemented with new data on histopathology, spore morphology, and 18s small subunit (ssu) ribosomal dna (rdna) sequence. plasmodia presented as both internal and external, raised, cyst-like lesions on the body wall of the peritoneal cavity and on the skin. the cysts contained numerous elongate, lanceolate myxospores, flattened parallel to the suture line. the spore body was 14.8 ± 1.1 µm ( ... | 2009 | 19575542 |
| differential susceptibility of blue catfish, ictalurus furcatus (valenciennes), channel catfish, i. punctatus (rafinesque), and blue x channel catfish hybrids to channel catfish virus. | | 2008 | 18086038 |
| differences in mortality, growth, lysozyme, and toll-like receptor gene expression among genetic groups of catfish exposed to virulent edwardsiella ictaluri. | survivorship to esc (enteric septicemia of catfish) varies among and within strains of commercially raised catfish, however the immunological basis for differences in susceptibility is not well-understood. we assessed the effect of pathogen challenge with edwardsiella ictaluri on five genetic groups of catfish by measuring both phenotypic response (mortality, pathogen levels, specific growth rate), and three measures of immune response, including lysozyme activity and mrna expression of two toll ... | 2008 | 18023209 |
| host-specific association between flavobacterium columnare genomovars and fish species. | a total of 90 flavobacterium columnare isolates were recovered from predominant wild fish species in the mobile river, alabama, usa. isolates were identified and confirmed by fatty acid methyl ester analysis and specific pcr amplification. genomovar ascription was performed using 16s-restriction fragment length polymorphism (rflp) analysis. the majority of genomovar i isolates were recovered from threadfin shad while genomovar ii isolates came from catfish (including channel and blue catfish). a ... | 2007 | 17706907 |
| microarray analysis of gene expression in the blue catfish liver reveals early activation of the mhc class i pathway after infection with edwardsiella ictaluri. | the acute nature of disease outbreaks in aquaculture settings has served to emphasize the importance of the innate immune response of fish for survival and led to the recent identification and characterization of many of its components. catfish, the predominant aquaculture species in the united states, is an important model for the study of the teleost immune system. however, transcriptomic-level studies of disease-related gene expression in catfish have only recently been initiated, and underst ... | 2008 | 17599411 |
| expression analysis of the acute phase response in channel catfish (ictalurus punctatus) after infection with a gram-negative bacterium. | the acute phase response (apr) is a set of metabolic and physiological reactions occurring in the host in response to tissue infection or injury and is a crucial component of the larger innate immune response. the apr is best characterized by dramatic changes in the concentration of a group of plasma proteins known as acute phase proteins (apps) which are synthesized in the liver and function in a wide range of immunity-related activities. utilizing a new high-density in situ oligonucleotide mic ... | 2007 | 17449095 |
| structural characterisation and expression analysis of toll-like receptor 2 gene from catfish. | toll-like receptors (tlrs) are important components of innate immunity. they were found to recognise specific structures on pathogens termed pathogen-associated molecular patterns (pamps) and utilise conserved signaling pathways to activate pro-inflammatory cytokines and type-1 interferons. in spite of much understanding gained from the mammalian systems, many fish tlrs are unknown. recent studies in japanese flounder as well as in zebrafish suggested that the ligand binding and activation of in ... | 2007 | 17116407 |
| toll-like receptor 3 and ticam genes in catfish: species-specific expression profiles following infection with edwardsiella ictaluri. | toll-like receptors (tlrs) are a family of transmembrane proteins that recognize specific pathogen-associated molecular patterns and use conserved signaling pathways to activate proinflammatory cytokines and type-1 interferons to fight infection. tlr3 in mammals is best known for its recognition of dsrna as ligand and its myd88-independent signaling. tlr3, upon recognition of dsrna, recruits and binds its adaptor protein tir domain-containing adapter molecule (ticam) 1. here we report the genomi ... | 2006 | 16969679 |
| the catfish liver-expressed antimicrobial peptide 2 (leap-2) gene is expressed in a wide range of tissues and developmentally regulated. | antimicrobial peptides (amps) are important components of the host's innate immune response against microbial invasion. the cysteine-rich amps such as defensin and hepcidin have been extensively studied, but the recently identified cysteine-rich liver-expressed antimicrobial peptide 2 (leap-2) has been characterized from only a few organisms. here we cloned and sequenced the leap-2 cdnas from both channel catfish and blue catfish. the leap-2 gene from channel catfish was also sequenced and chara ... | 2006 | 16310050 |
| cloning and characterization of myogenic regulatory genes in three ictalurid species. | we report sequence, tissue expression and map-position data for myogenin, myod1, myostatin and follistatin in three ictalurid catfish species: channel catfish (ictalurus punctatus), blue catfish (i. furcatus) and white catfish (ameiurus catus). these genes are involved in muscle growth and development in mammals and may play similar roles in catfish. amino acid sequences were highly conserved among the three ictalurid species (>95% identity), moderately conserved among catfish and zebrafish (app ... | 2004 | 15566463 |
| estima, a tool for est management in a multi-project environment. | single-pass, partial sequencing of complementary dna (cdna) libraries generates thousands of chromatograms that are processed into high quality expressed sequence tags (ests), and then assembled into contigs representative of putative genes. usually, to be of value, ests and contigs must be associated with meaningful annotations, and made available to end-users. | 2004 | 15527510 |
| analysis of a catfish gene resembling interleukin-8: cdna cloning, gene structure, and expression after infection with edwardsiella ictaluri. | chemokines are important mediators for innate immunity involved in recruitment, activation and adhesion of a variety of leukocyte types to inflammatory foci. while almost all chemokines have been identified from mammals, only a handful of fish chemokines have been identified. here we report molecular cloning, sequence analysis, and expression of a channel catfish gene resembling interleukin-8 (il-8). the gene has two alternatively spliced transcripts encoding 114 and 111 amino acids, respectivel ... | 2005 | 15450753 |
| multiple cc chemokines in channel catfish and blue catfish as revealed by analysis of expressed sequence tags. | chemokines represent a superfamily of chemotactic cytokines involved in recruitment, activation and adhesion of a variety of leukocyte types to inflammatory foci, as well as in the organization and maintenance of lymphoid organ architecture and in normal developmental processes. nearly all chemokines have been identified in human and mouse, but only a handful of fish chemokines have been identified. here we describe 14 distinct chemokines from channel catfish and blue catfish identified by analy ... | 2004 | 15309344 |
| sequence analysis and expression of a cxc chemokine in resistant and susceptible catfish after infection of edwardsiella ictaluri. | chemokines represent a superfamily of chemotactic cytokines involved in recruitment, activation and adhesion of a variety of leukocyte types to inflammatory foci. we cloned and sequenced the cdna of a cxc chemokine that is most similar to cxcl10 from channel catfish and blue catfish. sequence analysis of pcr amplicons from a single f1 hybrid catfish indicated that channel catfish and blue catfish may have a multigene family for the cxc chemokine. the catfish cxc chemokine was expressed in a wide ... | 2004 | 15043945 |
| putative snp discovery in interspecific hybrids of catfish by comparative est analysis. | in this study, we identified putative snp markers within genes by comparative analysis of expressed sequence tags (ests). comparison of 849 ests from blue catfish (ictalurus furcatus) with >11,000 ests from channel catfish (i. punctatus) deposited in genbank resulted in the identification of 1020 putative snps within 161 genes, of which 145 were nuclear genes of known function. the observed frequency of snps within ests of the two closely related catfish species was 1.32 snp per 100 bp. the majo ... | 2003 | 14687075 |
| microsatellite-containing genes from the channel catfish brain: evidence of trinucleotide repeat expansion in the coding region of nucleotide excision repair gene rad23b. | expansion of trinucleotide repeats within genes is well known to cause pathological conditions in humans. here we report a large number of genes containing simple sequence repeats (ssr) from the brain of channel catfish, of which a homologue of the rad23b gene was found to include (cca) trinucleotide repeats within its coding region. because of the importance of the rad23b gene in the nucleotide excision repair (ner) system, the catfish rad23b locus was further characterized. the (acc) repeats e ... | 2001 | 11716474 |
| prosthenystera oonastica n. sp. (digenea: callodistomidae) from ictalurid catfishes in southeastern united states and molecular evidence differentiating species in the genus across americas. | prosthenhystera oonastica n. sp. is described as a cryptic species from the gall bladder of three ictalurid catfishes, ictalurus punctatus (rafinesque), ictalurus furcatus (valenciennes), and pylodictis olivaris (rafinesque), in rivers in the southeastern united states. the species was originally named by wilmer a. rogers in 1979 but never formally described. material used for the description consists of two specimens of roger's original material and ten new specimens. we found no significant mo ... | 2015 | 25557746 |
| catfish hybrid ictalurus punctatus × i. furcatus exhibits higher resistance to columnaris disease than the parental species. | we present experimental data on susceptibility to columnaris disease, caused by the bacterium flavobacterium columnare, in hybrid catfish (female channel catfish ictalurus punctatus × male blue catfish i. furcatus) (c×b). under our experimental conditions, c×b hybrids were significantly more resistant to columnaris disease caused by the highly virulent strain of f. columnare bgfs-27 (genomovar ii) than channel catfish and blue catfish. channel and blue catfish cumulative mortalities after immers ... | 2012 | 22885516 |
| nutritional impacts on gene expression in the surface mucosa of blue catfish (ictalurus furcatus). | short-term feed deprivation is a common occurrence in both wild and farmed fish species, due to reproductive processes, seasonal variations in temperature, or in response to a disease outbreak. fasting can have dramatic physiological and biological consequences for fish, including impacts on mucosal immunity which can, in turn, change host susceptibility to pathogens. culture and selection of blue catfish (ictalurus furcatus) has gained importance as the production of a channel catfish×blue catf ... | 2014 | 24378224 |
| epidemiology of columnaris disease affecting fishes within the same watershed. | in the southeastern usa, columnaris disease (caused by flavobacterium columnare) typically affects catfish raised in earthen ponds from early spring until late summer. recently, unusually severe outbreaks of columnaris disease occurred at the e. w. shell fisheries center located in auburn, al, usa. during these outbreaks, catfish and other aquaculture and sport fish species that were in ponds located within the same watershed were affected. our objective was to investigate the genetic diversity ... | 2014 | 24991846 |
| comparative susceptibility of channel catfish, blue catfish, and their hybrid cross to experimental challenge with bolbophorus damnificus (digenea: bolbophoridae) cercariae. | the digenetic trematode bolbophorus damnificus has been implicated in significant losses in catfish aquaculture since the late 1990s. the complex life cycle sequentially involves the american white pelican pelecanus erythrorhynchos, the marsh rams horn snail planorbella trivolvis, and channel catfish ictalurus punctatus. research supports anecdotal reports from the industry, suggesting that the hybrid of channel catfish×blue catfish i. furcatus is less susceptible to disease agents that have bee ... | 2014 | 24895863 |
| efficacy of florfenicol for control of mortality associated with edwardsiella ictaluri in three species of catfish. | the efficacy of florfenicol for control of mortality associated with edwardsiella icatluri was studied in fingerlings of channel catfish ictalurus puntatus (delta strain), blue catfish i. furcatus (d&b strain), and a hybrid catfish (delta strain channel catfish × d&b strain blue catfish). on day 0, fish were immersion challenged in 65-l aquaria. for each of the three species of catfish, 10 aquaria were randomly assigned to two treatment groups, either treated with florfenicol at 0 mg/kg of body ... | 2015 | 26306332 |
| the nck and abi adaptor genes in catfish and their involvement in esc disease response. | adaptor proteins non-catalytic region of tyrosine kinase (nck) and abelson interactor (abi) are crucial for disease response. nck1 was identified to be a candidate gene for enteric septicemia of catfish (esc) disease resistance, and was speculated to play similar roles during esc and enteropathogenic escherichia coli (epec) pathogenicity. abi1 was reported as a positional candidate gene for bacterial cold water disease (bcwd) resistance in rainbow trout. in this study, three nck genes and six ab ... | 2017 | 28341353 |
| isolation of bacteroides from fish and human fecal samples for identification of unique molecular markers. | bacteroides molecular markers have been used to identify human fecal contamination in natural waters, but recent work in our laboratory confirmed cross-amplification of several human-specific bacteroides spp. assays with fecal dna from fish. for identification of unique molecular markers, bacteroides from human (n = 4) and fish (n = 7) fecal samples were cultured and their identities were further confirmed using rapid id 32a api strips. the 16s rdna from multiple isolates from each sample was pc ... | 2013 | 24313449 |
| early mucosal responses in blue catfish (ictalurus furcatus) skin to aeromonas hydrophila infection. | bacterial pathogens are well-equipped to detect, adhere to, and initiate infection in their finfish hosts. the mucosal surfaces of fish, such as the skin, function as the front line of defense against such bacterial insults that are routinely encountered in the aquatic environment. while recent progress has been made, and despite the obvious importance of mucosal surfaces, the precise molecular events that occur soon after encountering bacterial pathogens remain unclear. indeed, these early even ... | 2013 | 23337110 |
| marine nutrient contributions to freshwater apex predators. | recent investigations into the nutrient cycling of coastal ecosystems have suggested that migratory or anadromous fish could be important vectors of marine nutrients. anadromous fish have assimilated marine nutrients that would contribute to the nutrient budgets of freshwater systems by excretion, gamete release, or the decay of post-reproductive carcasses. however, the extent to which freshwater predators utilize marine material is not well understood. in systems where anadromous fish temporari ... | 2000 | 28308350 |
| using flow-ecology relationships to evaluate ecosystem service trade-offs and complementarities in the nation's largest river swamp. | large river systems are inextricably linked with social systems; consequently, management decisions must be made within a given ecological, social, and political framework that often defies objective, technical resolution. understanding flow-ecology relationships in rivers is necessary to assess potential impacts of management decisions, but translating complex flow-ecology relationships into stakeholder-relevant information remains a struggle. the concept of ecosystem services provides a bridge ... | 2015 | 25840699 |
| potential human health risk by metal(loid)s, 234,238u and 210po due to consumption of fish from the "luis l. leon" reservoir (northern méxico). | concentrations of as, cu, fe, hg, pb and zn and activity concentrations from 234,238u and 210po in water, fillet, liver and gills were determined in three stocked fish species from the luis l. leon reservoir, located in northern mexico. the considered species were lepomis cyanellus, cyprinus carpio and ictalurus furcatus. 238u and 234u activity concentration (ac) in fillet samples showed values of 0.007-0.014 and 0.01-0.02 bq∙kg-1 wet weight (ww), respectively. liver samples for l. cyanellus, c. ... | 2014 | 24968208 |
| molecular identification and expression analysis of a goose-type lysozyme (lysg) gene in yellow catfish pelteobagrus fulvidraco. | lysozymes, innate immunity molecules, play a vital role in immune response to pathogens. the yellow catfish pelteobagrus fulvidraco (siluriformes: bagridae) is an economically important fish in china. the aim of this study was to quantify expression of the p. fulvidraco lysg gene (a g-type lysozyme) in response to pathogen-associated molecular patterns (pamp) challenge. first, the p. fulvidraco lysg gene (pflysg) was cloned and characterized. the full-length cdna of pflysg is 1323 bp, including ... | 2016 | 27645907 |
| 18s rrna gene sequencing identifies a novel species of henneguya parasitizing the gills of the channel catfish (ictaluridae). | in the southeastern usa, the channel catfish ictalurus punctatus is a host to at least eight different species of myxozoan parasites belonging to the genus henneguya, four of which have been characterized molecularly using sequencing of the small subunit ribosomal rna (ssu rrna) gene. however, only two of these have confirmed life cycles that involve the oligochaete dero digitata as the definitive host. during a health screening of farm-raised channel catfish, several fish presented with deforme ... | 2014 | 25270236 |
| host-parasite relationships of rhabdochona kidderi pearse, 1936 (nematoda: rhabdochonidae) in fishes of the lacantún river in the lacandon rain forest of chiapas state, southern mexico, with a key to mexican species of rhabdochona railliet, 1916. | for the first time, the nematode rhabdochona kidderi kidderi pearse, 1936 (rhabdochonidae) was recorded from fishes of the lacantún river (usumacinta river basin) in the lacandon rain forest, chiapas state, southern mexico. amphilophus nourissati (allgayer) and theraps irregularis günther (both perciformes: cichlidae) were found to be the only definitive hosts in the locality, whereas eugerres mexicanus (steindachner) (perciformes: gerreidae), ariopsis sp., cathorops aguadulce (meek) and potomar ... | 2012 | 22488427 |
| a review of fish lectins. | lectins have been reported from various tissues of a diversity of fish species including japanese eel, conger eel, electric eel, bighead carp, gibel carp, grass carp, arabian gulf catfish, channel catfish, blue catfish, catfish, pike perch, perch, powan, zebrafish, toxic moray, cobia fish, steelhead trout, japanese trout, atlantic salmon, chinook salmon, olive rainbow smelt, rainbow smelt, white-spotted charr, tilapia, blue gourami, ayu, potca fish, spanish mackerel, gilt head bream, tench, roac ... | 2015 | 25929869 |