| nosocomial conjunctival ophthalmomyiasis with cochliomyia macellaria. | | 1991 | 2012163 |
| nosocomial nasal myiasis. | sixty-five fly maggots were retrieved from the nasal cavity of an unconscious 64-year-old man who had been admitted 18 days earlier with diabetic hyperosmolar coma. the larvae were identified as cochliomyia macellaria, an organism commonly associated with myiasis in the united states. the clinical time sequence indicates that this infection was acquired in the hospital. this incident provides further evidence that immobile and debilitated patients are at risk to acquire myiasis. | 1986 | 3754428 |
| a case of aural myiasis caused by cochliomyia macellaria (fabricius). | | 1968 | 4988429 |
| survival after submergence in the pupae of five species of blow flies (diptera: calliphoridae). | survival of pupae of known ages after 1 to 5 d of submersion under water was assessed by determining the emergence of protophormia terraenovae (robineau-desvoidy), calliphora vicina (robineau-desvoidy), cochliomyia macellaria (f.), phormia regina (meigen), and phaenicia sericata (meigen). survival after 1 d of submergence was < 14% in the white puparial stage, 0-100% in pharate adults nearing eclosion, and approximately 100% in pupae between these two stages. this pattern inversely tracks o2 con ... | 1994 | 7966181 |
| effect of the red imported fire ant (hymenoptera: formicidae) and carcass type on the daily occurrence of postfeeding carrion-fly larvae (diptera: calliphoridae, sarcophagidae). | red imported fire ants, solenopsis invicta buren, caused an increased in the proportion of gaps in the daily occurrence of postfeeding larvae of cochliomyia macellaria (f.) in carrion-baited traps. chrysomya rufifacies appeared later in the decomposition process and over a wider period of days in goat than in rabbit carcasses. the dependence of presence or absence of a carrion arthropod taxon at a given point in succession on interactions between other arthropods or carcass type must be consider ... | 1994 | 8158622 |
| theoretical dynamics of experimental populations of introduced and native blowflies (diptera:calliphoridae). | equilibrium dynamics in experimental populations of chrysomya megacephala (f.) and c. putoria (wiedemann), which have recently invaded the americas, and the native species cochliomyia macellaria (f.), were investigated using nonlinear difference equations. a theoretical analysis of the mathematical model using bifurcation theory established the combination of demographic parameters responsible for producing shifts in blowfly population dynamics from stable equilibria to bounded cycles and aperio ... | 1996 | 8699446 |
| dispersal and distribution of calliphoridae (diptera) immatures from animal carcasses in southern louisiana. | spatial distributions of calliphorid pupae (based on adult emergence) from swine carcasses located in woods and pasture were determined during 4 seasons in southern louisiana. adult emergence was substantially greater in pastures than woods for all seasons except winter. secondary screwworm, cochliomyia macellaria (f.), was the predominant calliphorid species collected ( > 95%) during summer and fall; whereas, black blow fly, phormia regina (meigen), was the primary species (86%) during spring. ... | 1996 | 8699464 |
| identification of screwworm species by polymerase chain reaction-restriction fragment length polymorphism. | restriction fragment length polymorphisms in polymerase chain reaction amplified fragments (pcr-rflp) of mitochondrial dna were used to differentiate species of new world screwworms (diptera: calliphoridae). twenty-seven restriction enzymes were screened on five regions of mtdna. eleven restriction fragment length patterns differentiated new world screwworm, cochliomyia hominivorax (coquerel), from secondary screwworm, cochliomyia macellaria (f.). five restriction fragment length patterns were p ... | 1996 | 8834744 |
| mitochondrial dna restriction site map of cochliomyia macellaria (diptera:calliphoridae). | the mitochondrial dna of the secondary screwworm, cochliomyia macellaria (f.), was cleaved with 15 restriction endonucleases to produce 54 restriction fragments. forty-three restriction sites recognized by 12 enzymes were assigned positions on a restriction site map. both the restriction fragments and the restriction site map were compared with published data from the screwworm, cochliomyia hominivorax (coquerel). the restriction site maps were aligned using a combination of highly conserved res ... | 1996 | 8840699 |
| effects of temperature on cochliomyia macellaria (diptera:calliphoridae) development. | growth curves were studied for the egg, larva, and pupa of the secondary screwworm, cochliomyia macellaria (f), under mean cyclic temperatures of 15.6, 21.1, 26.7, 32.2, and a constant 25.0 degrees c. development from egg to adult under all regimes ranged from 170 to 588 h. a constant temperature regime of 25 degrees c produced a range of pupation times from 92 to 142 h, with adult emergence ranging from 196 to 261 h. the maximal preferential temperature of 39 degrees c was determined for maggot ... | 1996 | 8961637 |
| [the synanthropy of the calliphorids (insecta, diptera) of curitiba, paraná, brazil]. | the calliphorids synantropics were studied in three different environments--urban, rural and forest area--in metropolitan region of curitiba, paraná, brazil during october and december of 1996 to estimate the abundance, preferences of the adults to baits (fresh fish, bird liver and human faeces) and the synanthropic index for the most abundant species. a total of 3399 calliphorids of 13 different species were sampled: phaenicia eximia (wiedemann, 1819), phaenicia cuprina (wiedemann, 1818), phaen ... | 1998 | 9697653 |
| seasonal colonization and decomposition of rat carrion in water and on land in an open field in south carolina. | decomposition and insect colonization of rat, rattus rattus l., carrion on land and in water were compared during summer and winter in a plowed field in northwestern south carolina. during winter, carcasses on land reached the dried-remains stage of decomposition, whereas carcasses in water reached the early-floating stage. during summer, carcasses in both habitats entered the final-remains stage of decomposition in 1-2 wk. fewer than 30 species of carrion insects were recorded from the carcasse ... | 1998 | 9775597 |
| evolution and structural organisation of mitochondrial dna control region of myiasis-causing flies. | this study reports the molecular characterization of the mtdna control region (called the a+t-rich region in insects) of five dipteran species which cause myiasis: cochliomyia hominivorax coquerel, cochliomyia macellaria fabricius, chrysomya megacephala fabricius, lucilia eximia wiedemann (diptera: calliphoridae) and dermatobia hominis linnaeus jr (diptera: oestridae). the control region in these species varies in length from 1000 to 1600 bp. two structural domains with specific evolutionary pat ... | 2000 | 10759315 |
| partial sequencing of the cytochrome oxydase b subunit gene i: a tool for the identification of european species of blow flies for postmortem interval estimation. | the identification of insects found on a dead body can lead to the estimation of the time of death (postmortem interval). we report an updated version of an established method based on sequence analysis of pcr products from a region of the cytochrome b oxidase subunit i mitochondrial gene of different members of the family calliphoridae, by sequencing six european species: lucilia sericata (meigen), lucilia caesar (linné), lucilia illustris (meigen), calliphora vicina (robineau-desvody), calliph ... | 2000 | 10914577 |
| characterization of the screwworm flies cochliomyia hominivorax and cochliomyia macellaria by pcr-rflp of mitochondrial dna. | the primary screwworm fly cochliomyia hominivorax (coquerel) (diptera: calliphoridae) is one of the most important insect pests of livestock in neotropical regions, whereas cochliomyia macellaria (fabricius) (diptera: calliphoridae), the secondary screwworm, is of medical and sanitary importance because of its role in the dissemination of pathogens. these two species share morphological similarities and both may occur in the same myiasis, but in different developmental stages. in this work, the ... | 2001 | 11434552 |
| insects of forensic significance in argentina. | records from forensic expertises and trappings with beef baits conducted in buenos aires, argentina (34 degrees 36's), show that the dominating species are widespread ones (calliphora vicina and phaenicia sericata), with different behaviour in each large latitudinal zone. it is suggested that the range of the yearly photoperiod variation has an influence in the behaviour of the blowflies, making up for differences in the succession patterns. the calliphorid blowflies cochliomyia macellaria and c ... | 2001 | 11457623 |
| spatio-temporal dynamics and transition from asymptotic equilibrium to bounded oscillations in chrysomya albiceps (diptera, calliphoridae). | the sensitivity of parameters that govern the stability of population size in chrysomya albiceps and describe its spatial dynamics was evaluated in this study. the dynamics was modeled using a density-dependent model of population growth. our simulations show that variation in fecundity and mainly in survival has marked effect on the dynamics and indicates the possibility of transitions from one-point equilibrium to bounded oscillations. c. albiceps exhibits a two-point limit cycle, but the intr ... | 2001 | 11500759 |
| distinction among the puparia of three blowfly species (diptera: calliphoridae) frequently found on unburied corpses. | calliphorid larvae are important in the decomposition of carrion. since these larvae are present in the primary stages of succession on carcasses, they may be important indicators of death time and the movement of corpses in homicide investigations. in this study we examined the morphological differences among puparia of chrysomya megacephala, c. putoria and cochliomyia macellaria. puparia of the three species (n=30, each) were obtained from the f2 generation bred in culture medium at 25 degrees ... | 2001 | 11562702 |
| seasonal patterns of arthropods occurring on sheltered and unsheltered pig carcasses in buenos aires province (argentina). | differences in the succession of insects and other arthropoda (invertebrate animals with jointed legs), on domestic pig carcasses placed under a roof and under the open sky have been studied in buenos aires province, argentina (latitude 34 degrees 45's) in all the seasons of the year. faunal associations proved different for each treatment in winter: the common bluebottle calliphora vicina was found in both, but on the sheltered carcass cochliomyia macellaria and the rare phaenicia cluvia were f ... | 2002 | 11955835 |
| flies (calliphoridae, muscidae) and beetles (silphidae) from human cadavers in cali, colombia. | adult specimens of cochliomyia macellaria, chrysomya megacephala, ch. rufifacies, lucilia sp. (calliphoridae), musca domestica (muscidae), oxelytrum discicolle (silphidae) and sarcophagidae were recovered from 12 human cadavers in cali, valle, colombia. information regarding these findings is presented. | 2002 | 11992165 |
| larval dispersal and predation in experimental populations of chrysomya albiceps and cochliomyia macellaria (diptera: calliphoridae). | in this study we investigated the larval dispersal associated with larval predation in experimental populations of chrysomya albiceps and cochliomyia macellaria. frequency distribution of sampling units (g test) in the substrate was used to evaluate variation in larval dispersal. an experimental acrylic channel (1 x 0.1 x 0.2 m) covered with wood shavings was used to observe larval dispersal prior to pupation. the acrylic channel was graduated at 0.05 m intervals, each representing a sampling un ... | 2003 | 12563480 |
| quantifying the potential pathogens transmission of the blowflies (diptera: calliphoridae). | to quantify the potential capability of transporting and passing infective pathogens of some blowflies (diptera: calliphoridae), mihályi's danger-index was calculated for seven species. the original equation was modified to include synanthropic information to discriminate between asynanthropic, hemisynanthropic, and eusynanthropic status. three groups were recognized, of which phaenicia cluvia and musca domestica proved the flies with lowest index value (d = 2.93 and 3.00 respectively); cochliom ... | 2003 | 12764436 |
| composition of the surface hydrocarbons from the vitelline membranes of dipteran embryos. | hydrocarbons were the major lipid class extracted by hexane from the vitelline membrane surface of dechorionated eggs of the house fly, musca domestica, the new world screwworm, cochliomyia hominivorax, the secondary screwworm, cochliomyia macellaria, the green bottle fly, phaenicia sericata, the sheep blow fly, lucilia cuprina and the mexican fruit fly, anastrepha ludens. the length of time the embryos must be exposed to hexane with or without a small amount of alcohol in order to attain permea ... | 2003 | 14529755 |
| factors related to the attraction of flies at a biosolids composting facility (bariloche, argentina). | the composting process is used to treat biosolids from the wastewater treatment plant of bariloche (nw patagonia, argentina). since 1998, an odourless, innocuous and stable organic amendment has been produced at the biosolids composting plant of bariloche. however, volatile compounds produced during this process, attract different vectors, mainly insects belonging to the order diptera, particularly in summer. to evaluate factors associated with the attraction of diptera to composting windrows, v ... | 2004 | 15207571 |
| evaluation of significance of bacteria in larval development of cochliomyia macellaria (diptera: calliphoridae). | bacteria were isolated and identified from the digestive tract of the secondary screwworm, cochliomyia macellaria (f.) (diptera: calliphoridae), and their role in the larval development of this insect was assessed in laboratory bioassays. the analysis of 16s rdna sequences revealed that the bacterial isolates represented four species: providencia sp., escherichia coli o157:h7 (escherich), enterococcus faecalis (orla-jensen), and ochrobactrum sp. (holmes). developmental assays demonstrated that c ... | 2006 | 17162944 |
| a checklist of arthropods associated with rat carrion in a montane locality of northern venezuela. | this is the first report of arthropods associated with carrion in venezuela, using laboratory bred rats (rattus norvegicus). rat carcasses were exposed to colonization by arthropods in neighboring montane savanna and cloud forest habitats in the state of miranda. the taxonomic composition of the arthropods varied between both ecosystems. scarabaeidae, silphidae, micropezidae, phoridae, vespidae and one species of ant, were collected only in the cloud forest. dermestes maculatus, chrysomya albice ... | 2008 | 17386987 |
| forensically important calliphoridae (diptera) associated with pig carrion in rural north-central florida. | a study to determine the relative abundance and seasonality of forensically important blow flies (diptera: calliphoridae) in rural north-central florida was conducted using pig carcasses (sus scrofa l.) as models for human bodies. seven species of calliphoridae were collected: lucilia coeruleiviridis (=phaenicia) (macquart), cochliomyia macellaria (f.), chrysomya rufifaces (macquart), phormia regina (meigen), chrysomya megacephala (f.), and a few specimens of calliphora livida hall, and callipho ... | 2007 | 17547239 |
| thermoregulation in larval aggregations of carrion-feeding blow flies (diptera: calliphoridae). | the growth and development of carrion-feeding calliphorid (diptera: calliphoridae) larvae, or maggots, is of great interest to forensic sciences, especially for estimation of a postmortem interval (pmi). the development rate of calliphorid larvae is influenced by the temperature of their immediate environment. heat generation in larval feeding aggregations (=maggot masses) is a well-known phenomenon, but it has not been quantitatively described. calculated development rates that do not include i ... | 2007 | 17547240 |
| [myiasis associated with some socioeconomic factors in five urban areas of the state of rio de janeiro]. | this was a descriptive epidemiological study with the aim of evaluating the occurrence of human myiasis in urban areas of four municipalities in the state of rio de janeiro. seventy-one patients who spontaneously sought attendance at primary healthcare units between october 1999 and october 2003 were examined. the disease was more prevalent among adults, including in individuals more than 51 years old (42.3%), and among children less than 10 years old (33.8%). from all the cases studied, 62% wer ... | 2007 | 17568884 |
| incidence of myiasis in panama during the eradication of cochliomyia hominivorax (coquerel 1858, diptera: calliphoridae) (2002-2005). | we present the results of a study on myiasis in panama during the first years of a cochliomyia hominivorax eradication program (1998-2005), with the aim of investigating the behavior of the flies that produce myiasis in animals and human beings. the hosts that registered positive for myiasis were cattle (46.4%), dogs (15.3%), humans (14.7%), birds (12%), pigs (6%), horses (4%), and sheep (1%). six fly species caused myiasis: dermatobia hominis (58%), phaenicia spp. (20%), cochliomyia macellaria ... | 2007 | 17923994 |
| species composition and seasonal succession of saprophagous calliphorids in a rural area of córdoba: argentina. | during 2004, four experiments were carried out, one each season, in order to determine the species composition and seasonal dynamics of calliphoridae in a rural area of córdoba (argentina). two pigs (sus scrofa l.), weighing approximately 8 kg each, were used in each experiment. they were killed with a blow to the head and immediately placed in a variant of the schoenly et al. (1991) trap. one pig was exposed in the shade and the other one under direct sunlight. the insect fauna was collected da ... | 2007 | 18064353 |
| insect succession and decomposition patterns on shaded and sunlit carrion in saskatchewan in three different seasons. | a study was conducted on decomposition and insect succession in the prairie ecozone of saskatchewan in the year 2000. eighteen domestic pig carcasses (42-79 kg) were employed as human models for applications to future homicide investigations in this region. two major variables were considered including the effect of season and habitat (sun versus shade). research was conducted over 25 weeks, spanning three seasons: spring, summer and fall. ambient temperature, internal carcass temperature, fauni ... | 2008 | 18662603 |
| amplified fragment length polymorphism used for inter- and intraspecific differentiation of screwworms (diptera: calliphoridae). | morphologically, early immature stages of the economically important pest called screwworms, cochliomyia hominivorax (coquerel) (diptera: calliphoridae), and non-pest secondary screwworms, cochliomyia macellaria (fabricius) (diptera: calliphoridae), are nearly indistinguishable. correct identification is crucial to the ongoing eradication and exclusion program protecting the united states, mexico and central america from reinvasion of screwworms persistent in south america and the caribbean. amp ... | 2009 | 19006578 |
| wing morphometry as a tool for correct identification of primary and secondary new world screwworm fly. | cochliomyia hominivorax and cochliomyia macellaria are endemic neotropical calliphoridae species. the former causes severe myiasis in hosts while the latter is sarcosaprophagous, but commonly found as a second invader in wounds. due to the morphological similarity between them and the potential losses that c. hominivorax represents for cattle breeders, the rapid and correct identification of these two species is very important. in addition to a correct identification of these species, a good kno ... | 2010 | 19302726 |
| characterization and utilization of microsatellite loci in the new world screwworm fly, cochliomyia hominivorax. | new world screwworm populations in north and central america have been the targets of virtually continuous eradication attempts by sterile insect technique (sit) since the 1950s. nevertheless, in some areas, such as jamaica, sit control programmes have failed. reasons for the failure of sit-based control programmes in some locations are unknown, but it has been hypothesized that failure may be related to mating incompatibility between sterile and wild fly populations or to the existence of sexua ... | 2009 | 19335825 |
| a preliminary study of insect fauna on pig carcasses located in sugarcane in winter in southeastern brazil. | minimum post-mortem intervals can be estimated based on analyses of the pattern of insect succession on a carcass. in order to successfully apply this approach, insect development rates must be considered with regard to local regional climatic conditions. this study is the first to analyse insect succession on carcasses decomposing in a sugarcane crop in brazil. in all seasons, chrysomya albiceps (wiedemann) (diptera: calliphoridae) and cochliomyia macellaria (fabricius) were frequent visitors d ... | 2009 | 19493195 |
| [description and key to the main species of calliphoridae (diptera) larvae of forensic importance from colombia]. | larvae of 13 blowfly species from colombia are described and an illustrated key for all them is presented. all larval instars of calliphora nigribasis macquart, cochliomyia macellaria (fabricius), chrysomya albiceps (wiedemann), chrysomya megacephala (fabricius), hemilucilia segmentaria (fabricius), hemilucilia semidiaphana (rondani), lucilia eximia (weidemann) are described, but the second and third instars of compsomyiops verena (walter), and only the third instar of calliphora vicina robineau ... | 2009 | 19618061 |
| insects (diptera) associated with cadavers at the institute of legal medicine in pernambuco, brazil: implications for forensic entomology. | increasing rates of unsolved homicides in brazil prompt the need for applied entomological data to be used as a complementary tool by criminal investigators. in that context, we analyzed the occurrence of forensically important insect species (order diptera) on 14 cadavers taken into the institute of legal medicine (ilm), in pernambuco, brazil, according to the conditions of the body and the pattern of colonisation by insects. simultaneously, we surveyed the diversity of insects in the surroundi ... | 2010 | 20181449 |
| a checklist of calliphoridae blowflies (insecta, diptera) associated with a pig carrion in central brazil. | although the cerrado is the second major brazilian biome, few studies have been undertaken about its entomofauna. blowflies have an important role in forensic entomology, helping in the determination of postmortem intervals. the main goal of this exploratory study was to identify and to catalog the blowfly species associated with a pig carcass. the study was conducted in a pasture in brasília, distrito federal. a pig (sus scrofa) was killed with a .22 caliber shot in the frontal region of the he ... | 2010 | 20666916 |
| effects of temperature and tissue type on the development of cochliomyia macellaria (diptera: calliphoridae). | the secondary screwworm, cochliomyia macellaria (fabricius), was reared on either equine gluteus muscle or porcine loin muscle at 20.8 degrees c, 24.3 degrees c, and 28.2 degrees c. c. macellaria needed approximately 35% more time to complete development when reared at 20.8 than 28.2 degrees c. furthermore, larval growth and weight over time did not differ between larvae reared on equine versus porcine muscle. this study is the second in the united states to examine the development of c. macella ... | 2010 | 20939391 |
| rehydration of forensically important larval diptera specimens. | established procedures for collecting and preserving evidence are essential for all forensic disciplines to be accepted in court and by the forensic community at large. entomological evidence, such as diptera larvae, are primarily preserved in ethanol, which can evaporate over time, resulting in the dehydration of specimens. in this study, methods used for rehydrating specimens were compared. the changes in larval specimens with respect to larval length and weight for three forensically importan ... | 2011 | 21337957 |
| abundance and seasonality of cochliomyia macellaria (diptera: calliphoridae) in southern pantanal, brazil. | cochliomyia macellaria (diptera: calliphoridae), known as the secondary screwworm, occurs in the americas and has medical-veterinary and forensic importance. this study aimed to describe the seasonal fluctuation of this species in the pantanal region, central-western brazil. from december 2004 to november 2007 fly catches using four windoriented traps baited with decaying bovine liver were carried out at the nhumirim ranch, nhecol+óndia subregion, southern pantanal. traps remained active through ... | 2011 | 21439228 |
| epidemiological study of myiases in the hospital do andara+¡, rio de janeiro, including reference to an exotic etiological agent. | myiases are infestations with dipteran larvae in both necrosed and living tissues, the food source of these insects. these illnesses occur in warm humid climates, and are most frequent in developing countries. we assessed the epidemiological aspects and the influence of climate on the occurrence of myiases and the bioagents in patients admitted to the federal hospital do andara+¡ in rio de janeiro from february 2007 to 2008. the influence of abiotic factors (temperature, humidity, and rainfall) ... | 2011 | 21710036 |
| blow flies (diptera: calliphoridae) survive burial: evidence of ascending vertical dispersal. | this study was undertaken to determine if immature blow flies could complete development following burial and emerge from the soil as adults. two species of blow flies, cochliomyia macellaria and protophormia terraenovae, were placed at three depths and at three different life stages, in a simulated burial to evaluate the impact of soil on ascending vertical dispersal and fly survival. in soil columns, immature stages of each species were covered with 5, 25 and 50cm of soil. emerging adult flies ... | 2011 | 21890294 |
| a comparison of blow fly (diptera: calliphoridae) and beetle (coleoptera) activity on refrigerated only versus frozen-thawed pig carcasses in indiana. | to have large numbers of replicates in forensic entomology research, it is often necessary to freeze carcasses and thaw them before use in experiments. research on the effects of freezing on decomposition is minimal in the literature and the effect that it has on insect (e.g., blow fly, beetle) activity is virtually unknown. the objective of this study was to compare the differences in insect activity occurring on refrigerated only versus frozen-thawed pig carcasses, and to characterize the asso ... | 2011 | 22238884 |
| selection and validation of reference genes for functional studies in the calliphoridae family. | the genera cochliomyia and chrysomya contain both obligate and saprophagous flies, which allows the comparison of different feeding habits between closely related species. among the different strategies for comparing these habits is the use of qpcr to investigate the expression levels of candidate genes involved in feeding behavior. to ensure an accurate measure of the levels of gene expression, it is necessary to normalize the amount of the target gene with the amount of a reference gene having ... | 2014 | 25373149 |
| bacterial volatiles attract gravid secondary screwworms (diptera: calliphoridae). | bovine blood inoculated and incubated with bacteria was tested to determine if secondary screwworm, cochliomyia macellaria (f.), would be attracted to the incubated substrate for oviposition. five species of bacteria, klebsiella oxytoca (flugge), proteus mirabilis hauser, proteus vulgaris hauser, providencia rettgeri hadley, elkins and caldwell, and providencia stuartii ewing, previously isolated from animal wounds infested by primary screwworms, cochliomyia hominivorax (coquerel), were used. in ... | 2016 | 26748982 |
| first record of larval secretions of cochliomyia macellaria (fabricius, 1775) (diptera: calliphoridae) inhibiting the growth of staphylococcus aureus and pseudomonas aeruginosa. | maggot debridement therapy (mdt) consists on the intentional and controlled application of sterilized larvae of the order diptera on necrotic skin lesions with the purpose of cleaning necrotic tissue and removing pathogenic bacteria. during mdt, a marked antimicrobial activity has been reported in literature specially associated with antibacterial substances from lucilia sericata (meigen); however, regarding cochliomyia macellaria (fabricius), little is known. this study aimed to evaluate in vit ... | 2017 | 27696133 |
| an artificial diet for rearing cochliomyia macellaria (diptera: calliphoridae). | larvae of the secondary screwworm, cochliomyia macellaria (f.), feed on carrion and may sometimes cause animal myiasis. they have been reared in the laboratory on various animal tissues to study their growth and development because of their importance in forensic science. we use secondary screwworms in our laboratory for preliminary experiments as a model for the primary screwworm, cochliomyia hominivorax (coquerel), which has been eradicated from the united states. c. macellaria larvae reared o ... | 2013 | 24020312 |
| diptera of medico-legal importance associated with pig carrion in a tropical dry forest. | the diversity of necrophagous diptera is largely unknown in seasonally dry tropical forests, despite their medical, veterinary, and forensic relevance. we performed a study in the dry caatinga forest exclusive to brazil in order to assess the diversity and temporal pattern of diptera species using pig carcasses as substrates. adults were collected daily until complete skeletonization. we collected 17,142 adults from 18 families, 10 of which comprise species with known necrophagous habits. the mo ... | 2016 | 27330102 |
| assessment of the abundance and diversity of calliphoridae and sarcophagidae (diptera) in sites with different degrees of human impact in the iberá wetlands (argentina). | the aim of the present study was to explore the diversity and describe the spatial distribution of calliphoridae and sarcophagidae along an urbanization gradient in the iberá wetlands. the flies were collected at 18 sampling points, three per site. in total, 3,318 flies were collected (3,077 blow flies and 241 flesh flies), distributed in 13 genera and 33 species. calliphoridae was the most abundant, comprised 92.74% of all adult flies. chrysomya albiceps (wiedemann), cochliomyia macellaria (f.) ... | 2016 | 27106933 |
| intraguild predation influences oviposition behavior of blow flies (diptera: calliphoridae). | the objective of the present study was to determine whether blow flies (diptera: calliphoridae) are able to identify larvae of an intraguild predator species in the substrate and avoid laying eggs there. blow flies oviposited in traps with different treatments: substrate only and substrate with larvae of chrysomya albiceps (wiedemann, 1819), chrysomya megacephala (fabricius, 1794), or chrysomya putoria (wiedemann, 1830). ch. megacephala, ch. putoria, and lucilia eximia (wiedemann, 1819) avoided ... | 2016 | 26888288 |
| distribution and abundance of necrophagous flies (diptera: calliphoridae and sarcophagidae) in maranhão, northeastern brazil. | this study aimed at surveying the local calliphorid and sarcophagid species in maranhão state (brazil) to determine their distribution and abundance, as well as the distribution of exotic chrysomya species. in total, 18,128 calliphorid specimens were collected, distributed in 7 genera and 14 species. the species hemilucilia semidiaphana (rondani, 1850) and paralucilia paraensis (mello, 1969) were new state records. chrysomya albiceps (wiedemann, 1819) and cochliomyia macellaria (f., 1775) were t ... | 2015 | 26078304 |
| blowflies (diptera, calliphoridae) associated with pig carcasses in a caatinga area, northeastern brazil. | studies that focused on calliphoridae associated with pig carcasses are abundant in southern and southeastern brazil; however, there are few in northeast. here, we present an inventory of the blowfly species associated with the stages of decomposition of pig carcasses in a caatinga area during dry and rainy seasons. the study took place at the private reserve for the environmental inheritance "fazenda almas," state of paraíba, brazil. using a modified version of the shannon trap, 32,909 adult sp ... | 2014 | 27193518 |
| effect of lunar phases, tides, and wind speed on the abundance of diptera calliphoridae in a mangrove swamp. | abiotic factors, such as lunar phases and tides, have a significant effect on insect development. reproduction and immature development are usually interlinked to these abiotic factors. the tide is at its highest levels at full moon or new moon, hindering the feeding of the immature or causing their drowning. the oviposition by adult females is also compromised on these days because much of the available food is submerged. another important abiotic factor is the wind, which displaces odoriferous ... | 2014 | 27193403 |
| an update of the blow flies (diptera: calliphoridae) of the galápagos islands, and first record of chrysomya rufifacies (macquart) from mainland ecuador. | seven species of calliphoridae are reported from the galápagos islands, ecuador: lucilia pionia (walker), l. setosa (james), l. deceptor (curran), l. eximia (wiedemann), cochliomyia macellaria (fabricius), chrysomya albiceps (wiedemann), and chrysomya megacephala (fabricius). lucilia eximia is newly recorded from the islands. lucilia sp. near pionia is recorded from the island of española. the distribution and collection records of these species are discussed and listed, and a key to their ident ... | 2013 | 25113695 |
| the classic episode of biological invasion: cochliomyia macellaria (fabricius, 1775) versus chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) - evaluation of the biotic potential. | the classic episode of biological invasion: cochliomyia macellaria (fabricius, 1775) versus chrysomya megacephala (fabricius, 1794) (diptera: calliphoridae) - evaluation of the biotic potential. the biotic of the native fly cochliomyia macellaria and the exotic chrysomya megacephala was compared, through the reproductive capacity and longevity. the experiment took place in a climatized chamber, with four repetitions of 15 grouped couples of each species. the posture, egg mass weight, and death o ... | 2016 | 27508990 |
| decomposition of concealed and exposed porcine remains in the north carolina piedmont. | we examined the decomposition and subsequent insect colonization of small pig carrion (sus scrofa (l.)) placed in concealed and open environments during spring, summer, and fall in raleigh, north carolina, as a model for juvenile human remains. remains were concealed in simulated attics in three manners, ranging from minimal to well-concealed. concealment had a significant effect on the insect community colonizing the remains across all three seasons; the beetles necrobia rufipes (degeer) (cleri ... | 2016 | 26590193 |
| larval distribution and behavior of chrysomya rufifacies (macquart) (diptera: calliphoridae) relative to other species on florida black bear (carnivora: ursidae) decomposing carcasses. | larval interactions of dipteran species, blow flies in particular, were observed and documented daily over time and location on five black bear carcasses in gainesville, fl, usa, from june 2002 - september 2004. cochliomyia macellaria (fabricius) or chrysomya megacephala (fabricius) larvae were collected first, after which chrysomya rufifacies (macquart) oviposited on the carcasses in multiple locations (i.e., neck, anus, and exposed flesh) not inhabited already by the other blow fly larvae. wit ... | 2014 | 27193400 |
| conservation and sex-specific splicing of the transformer gene in the calliphorids cochliomyia hominivorax, cochliomyia macellaria and lucilia sericata. | transformer (tra) promotes female development in several dipteran species including the australian sheep blowfly lucilia cuprina, the mediterranean fruit fly, housefly and drosophila melanogaster. tra transcripts are sex-specifically spliced such that only the female form encodes full length functional protein. the presence of six predicted tra/tra2 binding sites in the sex-specific female intron of the l. cuprina gene suggested that tra splicing is auto-regulated as in medfly and housefly. with ... | 2013 | 23409170 |
| identification of oviposition attractants of the secondary screwworm, cochliomyia macellaria (f.) released from rotten chicken liver. | the secondary screwworm, cochliomyia macellaria (f.), is an important blowfly species affecting both livestock and humans. it can transmit pathogenic disease agents mechanically and is an agent of facultative myiasis, which leads to economic losses. the adult flies are attracted to decomposing carcasses, carrion, or rotten meat in order to deposit their eggs, and the hatched larvae develop on these decaying organic materials. this research was aimed to identify volatiles emitted from rotten chic ... | 2013 | 24174348 |
| life history of cochliomyia macellaria (fabricius, 1775) (diptera, calliphoridae), a blowfly of medical and forensic importance. | the life history traits of blow fly cochliomyia macellaria (fabricius, 1775) was studied under semi-controlled laboratory conditions at 29.14°c temperature, 72.53% relative humidity, and 12-h photoperiod. the raw data were analyzed based on the age-stage, two-sex life table, considering the development rates among individuals of both sexes. cochliomyia macellaria survival rate was 0.43 (♂) and 0.40 (♀), while life expectancy was 17.9 (♂) and 20.9 (♀) days, for adult males and females, respective ... | 2017 | 28265808 |
| identification and characterization of micrornas in the screwworm flies cochliomyia hominivorax and cochliomyia macellaria (diptera: calliphoridae). | micrornas (mirnas) are small noncoding rnas that modulate gene expression through post-transcriptional regulation. here, we report the identification and characterization of mirnas in two closely related screwworm flies with different feeding habits: cochliomyia hominivorax and cochliomyia macellaria. the new world screwworm, c. hominivorax, is an obligatory parasite of warm-blooded vertebrates, whereas the secondary screwworm, c. macellaria, is a free-living organism that feeds on decaying orga ... | 2017 | 27775856 |
| development and validation of a new technique for estimating a minimum postmortem interval using adult blow fly (diptera: calliphoridae) carcass attendance. | understanding the onset and duration of adult blow fly activity is critical to accurately estimating the period of insect activity or minimum postmortem interval (minpmi). few, if any, reliable techniques have been developed and consequently validated for using adult fly activity to determine a minpmi. in this study, adult blow flies (diptera: calliphoridae) of cochliomyia macellaria and chrysomya rufifacies were collected from swine carcasses in rural central texas, usa, during summer 2008 and ... | 2015 | 25367772 |
| quantifying pteridines in the heads of blow flies (diptera: calliphoridae): application for forensic entomology. | in forensic cases involving entomological evidence, establishing the postcolonization interval (post-ci) is a critical component of the investigation. traditional methods of estimating the post-ci rely on estimating the age of immature blow flies (diptera: calliphoridae) collected from remains. however, in cases of delayed discovery (e.g., when remains are located indoors), these insects may have completed their development and be present in the environment as adults. adult fly collections are o ... | 2017 | 28942377 |
| nonconsumptive effects of predatory chrysomya rufifacies (diptera: calliphoridae) larval cues on larval cochliomyia macellaria (diptera: calliphoridae) growth and development. | forensic entomologists often rely on development data associated with a given species to estimate when it colonized human or other vertebrate remains. in most instances, these development studies are based on single species reared in isolation in the laboratory. this study examined the impact of excretions and secretions (es) associated with third-instar chrysomya rufifacies (macquart), a predator, on the development of its prey, cochliomyia macellaria (f.). not surprisingly, ch. rufifacies es d ... | 2017 | 28541462 |
| methods for external disinfection of blow fly (diptera: calliphoridae) eggs prior to use in wound debridement therapy. | maggot debridement therapy (mdt) is the use of the larval stage of flies (i.e., calliphoridae) to remove necrotic tissue and disinfect wounds. effective mdt requires aseptic technique to prevent the unintentional introduction of pathogenic bacteria into a wound to be debrided; yet the external surface of calliphoridae eggs is often heavily contaminated with bacteria. studies of external disinfection of dipteran eggs have been reported, but neither their efficacy nor effect on egg viability has b ... | 2016 | 27041484 |
| hydrocarbon profiles throughout adult calliphoridae aging: a promising tool for forensic entomology. | blow flies (diptera: calliphoridae) are typically the first insects to arrive at human remains and carrion. predictable succession patterns and known larval development of necrophagous insects on vertebrate remains can assist a forensic entomologist with estimates of a minimum post-mortem interval (pmimin) range. however, adult blow flies are infrequently used to estimate the pmimin, but rather are used for a confirmation of larval species identification. cuticular hydrocarbons have demonstrated ... | 2014 | 25447177 |
| resource use by an introduced and native carrion flies. | the carrion fly chrysomya rufifacies has recently been introduced to north america. larvae of this species are facultative predators on other carrion larvae, and are known to reduce populations of the new world fly cochliomyia macellaria in the laboratory and in certain field situations. in order to identify conditions under which native taxa might avoid interaction with the invader, we examined broad patterns of resource use by capturing postfeeding larvae as they left a carcass. the calliphori ... | 1994 | 28313964 |
| molecular phylogeny of the forensically important genus cochliomyia (diptera: calliphoridae). | cochliomyia townsend includes several abundant and one of the most broadly distributed, blow flies in the americas, and is of significant economic and forensic importance. for decades, cochliomyia hominivorax (coquerel) and cochliomyia macellaria (fabricius) have received attention as livestock parasites and primary indicator species in forensic entomology. however, cochliomyia minima shannon and cochliomyia aldrichi del ponte have only been subject to basic taxonomy and faunistic studies. here ... | 2016 | 27563274 |
| physical and physiological factors influence behavioral responses of cochliomyia macellaria (diptera: calliphoridae) to synthetic attractants. | volatile chemicals from waste artificial larval media as well as from bovine blood inoculated with bacteria isolated from screwworm-infested wounds attract gravid females of cochliomyia hominivorax coquerel and cochliomyia macellaria (f.). chemicals identified from volatiles are dimethyl disulfide, dimethyl trisulfide, phenol, p-cresol, and indole; a blend of these attracted females to oviposit. present studies investigated the effectiveness of these compounds, either in a blend or individually ... | 2017 | 28535247 |
| sequence-characterized amplified regions that differentiate new world screwworms from other potential wound-inhabiting flies. | new world screwworms, cochliomyia hominivorax (coquerel, 1858), were once devastating pests of warm-blooded animals in the united states before they were successfully eradicated using the sterile insect technique. guarding against the introduction of screwworms to north america or any other screwworm-free area relies on rapid, reliable identification of suspected cases. in the current study, the dna from excised markers generated by randomly amplified polymorphic dna polymerase chain reaction wa ... | 2015 | 25387845 |
| volatiles from waste larval rearing media attract gravid screwworm flies (diptera: calliphoridae) to oviposit. | gravid screwworm flies, cochliomyia hominivorax (coquerel), are attracted to the volatiles from waste larval rearing media to deposit eggs. studies were conducted to identify volatile chemicals from the waste larval media and determine their effectiveness to attract gravid flies to oviposit. volatiles were collected using solid-phase microextraction method, and five active chemicals, namely, dimethyl disulfide, dimethyl trisulfide, phenol, p-cresol, and indole, were identified using gas chromato ... | 2014 | 24897851 |
| morphological and cytochemical aspects of spermatozoa in the genus cochliomyia (diptera: calliphoridae). | the objective of this study was to characterize, structurally and ultrastructurally, the spermatozoa of the screwworm flies cochliomyia hominivorax and cochliomyia macellaria. to visualize the ultrastructure of microtubules and identify basic proteins, techniques such as the tannic acid fixation and the cytochemical method of ethanolic phosphotungstic acid (epta) were used. these methods of fixation are important because they reinforce the evidence of the protofilaments present in the microtubul ... | 2012 | 22997238 |
| dipteran diversity and ecological succession on dead pigs in contrasting mountain habitats of chiapas, mexico. | a comparative study of diptera communities associated with pig carcasses was carried out in urban and natural settings in tropical mountain habitats of southern mexico. schoenly-type traps were used to set out pig carcasses during 68 d of sampling effort. in total, 1,703 adult diptera specimens were collected in both localities combined, and recording a total of 30 (morpho) species and 19 families. compared to natural habitats, a significantly greater diversity in urban habitats, as well as a fa ... | 2018 | 29186358 |
| insecticide activity of baccharis dracunculifolia essential oil against cochliomyia macellaria (diptera: calliphoridae). | the ethnobotanical uses of brazilian plants for different injuries and diseases conjoined with local rich biodiversity represent an important resource for research and development. this study aimed to characterise bdeo and its in vitro activity on the third instar larvae (l3) of cochliomyia macellaria. groups of 20 l3 were placed on filter paper impregnated with increasing concentrations of 5-30% (v/v), equivalent to 0.79-4.77 μl/cm2, solubilised in ethanol or acetone. the major constituents of ... | 2017 | 29052447 |
| factors affecting species identifications of blow fly pupae based upon chemical profiles and multivariate statistics. | alternative methods for the identification of species of blow fly pupae have been developed over the years that consist of the analyses of chemical profiles. however, the effect of biotic and abiotic factors that could influence the predictive manner for the tests have not been evaluated. the lipids of blowfly pupae (cochliomyia macellaria, lucilia cuprina, lucilia sericata, and phormia regina) were extracted in pentane, derivatized, and analyzed by total-vaporization solid phase microextraction ... | 2017 | 28398264 |
| post-colonization interval estimates using multi-species calliphoridae larval masses and spatially distinct temperature data sets: a case study. | common forensic entomology practice has been to collect the largest diptera larvae from a scene and use published developmental data, with temperature data from the nearest weather station, to estimate larval development time and post-colonization intervals (pcis). to evaluate the accuracy of pci estimates among calliphoridae species and spatially distinct temperature sources, larval communities and ambient air temperature were collected at replicate swine carcasses (n = 6) throughout decomposit ... | 2017 | 28375172 |
| forensic entomology: evaluating uncertainty associated with postmortem interval (pmi) estimates with ecological models. | estimates of insect age can be informative in death investigations and, when certain assumptions are met, can be useful for estimating the postmortem interval (pmi). currently, the accuracy and precision of pmi estimates is unknown, as error can arise from sources of variation such as measurement error, environmental variation, or genetic variation. ecological models are an abstract, mathematical representation of an ecological system that can make predictions about the dynamics of the real syst ... | 2016 | 27247349 |
| evaluation of conventional therapeutic methods versus maggot therapy in the evolution of healing of tegumental injuries in wistar rats with and without diabetes mellitus. | larval therapy consists on the application of sterilized carrion flies larvae, reared in laboratory, on acute, chronic, and/or infected wounds in order to promote healing. conventional methods for treating injuries include mechanical debridement or silver-based dressings; however, they are not always effective for wound healing. larval therapy is a feasible and safe treatment for therapeutic application and, in many cases, the only and the most recommended alternative for difficult healing injur ... | 2016 | 26976407 |
| evaluation of larval density cochliomyia macellaria f. (diptera: calliphoridae) for therapeutic use in the recovery of tegumentar injuries. | larval therapy (lt) is the application of carrion flies (diptera) sterile larvae on chronic or infected wounds to promote or accelerate the healing process. high cost and the development of resistance by certain groups of pathogenic bacteria to these drugs encouraged the resurgence of lt, currently used in approximately 20 countries and more recently in brazil. this study evaluated the behavior and larval density of cochliomyia macellaria f. (calliphoridae), one of the most appropriate species f ... | 2015 | 26022143 |
| histological patterns in healing chronic wounds using cochliomyia macellaria (diptera: calliphoridae) larvae and other therapeutic measures. | the healing process occurs due to the interaction of cellular, molecular, and biochemical events. regarding lesions difficult to heal, especially in immunocompromised patients, monitoring and intervention to promote healing is a constant focus of research. another aggravating factor is the increase in the number of reported cases of microbial resistance, indicating that various dressings and drugs have been increasingly inefficient. larval therapy (lt) involves the application of sterile fly lar ... | 2015 | 25903008 |
| description of third instars of cochliomyia minima (diptera: calliphoridae) from west indies, and updated identification key. | the blow fly cochliomyia minima shannon is endemic to the caribbean, and it has great potential for forensic applications because of its abundance and broad distribution in the region. however, its larval stages are unknown. here, i update previously published identification keys by describing for the first time the morphology of c. minima larvae. the larvae of c. minima are found to be very similar to those of cochliomyia macellaria f., but the former can be easily identified by the oral scleri ... | 2014 | 25276936 |
| ultrastructure of immature stages of cochliomyia macellaria (diptera: calliphoridae), a fly of medical and veterinary importance. | cochliomyia macellaria (diptera: calliphoridae) is known as the secondary screwworm because it causes secondary or facultative myiasis when the larvae feed on necrotic tissues. this fly has a significant medical and veterinary importance since it has been reported to transport eggs of dermatobia hominis (human botfly), which can cause significant economic losses to livestock. since this screwworm has been collected colonizing both pig carcasses and human cadavers, it is considered one of the mos ... | 2014 | 25028212 |
| evaluating the utility of hexapod species for calculating a confidence interval about a succession based postmortem interval estimate. | carrion insect succession patterns have long been used to estimate the postmortem interval (pmi) during a death investigation. however, no published carrion succession study included sufficient replication to calculate a confidence interval about a pmi estimate based on occurrence data. we exposed 53 pig carcasses (16±2.5 kg), near the likely minimum needed for such statistical analysis, at a site in north-central indiana, usa, over three consecutive summer seasons. insects and collembola were s ... | 2014 | 24905150 |
| developmental variation among cochliomyia macellaria fabricius (diptera: calliphoridae) populations from three ecoregions of texas, usa. | forensic entomologists rely on published developmental datasets to estimate the age of insects developing on human remains. currently, these datasets only represent populations of targeted insects from specific locations. however, recent data indicate that populations can exhibit genetic variation in their development, including signatures of local adaptation demonstrated by regionally distinct plastic responses to their environments. in this study, three geographically distinct populations of t ... | 2014 | 24811885 |
| preliminary study of the succession pattern of necrobiont insects, colonising species and larvae on pig carcasses in temuco (chile) for forensic applications. | knowledge regarding the succession patterns of insects that visit carcasses as well as the other arthropod that colonise them and analysis of the parameters that are associated with larvae allow calculation of the minimum postmortem interval (pmi). this information is obtained from experiments carried out under specific geoclimatic conditions, which determine their application in forensic environments under similar conditions. the field study presented here is the first in chile to analyse the d ... | 2012 | 22578807 |