| molecular cloning and restriction enzyme mapping of avian adenovirus type 8 dna. | avian adenovirus (aav) type 8 was cultured in an avian hepatoma cell line designated ch-sah and the viral dna extracted and purified. restriction enzyme analysis of viral dna using the endonucleases apai, ecori, hindiii, kpni, noti, spei, stui and xbai was carried out, and fragments representing the entire genome were cloned. according to the restriction enzyme fragments, the size of the aav type 8 genome was calculated to be 44.7 kb. subcloning of viral dna fragments and hybridization studies u ... | 1996 | 8896244 |
| the orf rtl1 transcript of fowl adenovirus type-8 is spliced and truncated at late stages of the virus replication cycle. | two transcription products were found for the open reading frame (orf) rtl1 located near the right terminus of the fowl adenovirus type-8 genome. the larger transcript, which was transcribed mostly during the early stage of the virus infection, contains the complete sequence (933 nucleotides) of the predicted orf from the genomic dna sequence encoding a 311 amino acid (aa) polypeptide. in contrast, the shorter transcript, which was more predominant at the late stage of the infection, was missing ... | 2000 | 10872874 |
| the complete nucleotide sequence of fowl adenovirus type 8. | the fowl adenovirus type 8 (fadv-8) genome was sequenced and found to be 45063 nucleotides in length, the longest adenovirus (adv) genome for which the complete nucleotide sequence has been determined so far. no regions homologous to early regions 1, 3 and 4 (e1, e3 and e4) of mastadenoviruses were recognized. gene homologues for early region 2 (e2) proteins, intermediate protein iva2 and late proteins were found by their similarities to protein sequences from other advs. however, sequences homo ... | 2000 | 10859390 |
| growth characteristics of fowl adenovirus type 8 in a chicken hepatoma cell line. | fowl adenoviruses, many of which appear to be non-pathogenic, are ubiquitous in birds. in addition, the genome of these viruses is large, making them ideal candidates for construction as vectors for foreign genes. current methods to cultivate fowl adenoviruses use primary cell cultures derived from embryonated chicken eggs. in order to provide a more suitable culture method, the growth of fowl adenovirus type 8 (fadv-8) was investigated in ch-sah, a continuous hepatoma cell line. a one step grow ... | 1998 | 9763123 |
| sequence and transcriptional analysis of terminal regions of the fowl adenovirus type 8 genome. | fowl adenovirus (fadv) type 1, celo strain has no homologies to mastadenovirus e1a, e1b, e3 and e4, which regulate virus gene expression, dna replication and virus-host interaction. similarly, the right 5 kb and left 15 kb ends of celo virus dna are non-homologous to mastadenoviruses. to compare celo virus with another fadv, 7.5 kb of the left and 17 kb of the right ends of fadv type 8 (strain a-2a) were sequenced and nine and 17 open reading frames (orfs), respectively, were found. this fadv-8 ... | 1998 | 9780058 |