Publications

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the role of cattle as hosts of glossina longipennis at galana ranch, south-eastern kenya.glossina longipennis were recorded visiting and engorging on cattle in an enclosure and on a single ox in a crush using transparent electrocuting nets in an incomplete ring. of the total flies caught, 3-6% of males and 5-6% of females in the total catches were engorged (a feeding success rate of up to 16.6% and 12.6%, respectively, depending on assumptions made about the proportion which had an opportunity to feed). direct observation of tsetse from an observation pit showed 57% landing on the f ...19968994134
molecular characterization of a tsetse fly midgut proteolytic lectin that mediates differentiation of african trypanosomes.differentiation of bloodstream-form trypanosomes into procyclic (midgut) forms is an important first step in the establishment of an infection within the tsetse fly. this complex process is mediated by a wide variety of factors, including those associated with the vector itself, the trypanosomes and the bloodmeal. as part of an on-going project in our laboratory, we recently isolated and characterized a bloodmeal-induced molecule with both lectin and trypsin activities from midguts of the tsetse ...200616551548
control of glossina longipennis (diptera: glossinidae) by insecticide-treated targets at galana ranch, kenya, and confirmation of the role of g. longipennis as a vector of cattle trypanosomiasis.glossina longipennis corti was studied in galana ranch, kenya over a four year period, in two areas (tank e and lali) where the species was abundant and other species were absent or scarce. there was active transmission of trypanosomiasis to cattle in both areas, the parasite species being trypanosoma vivax ziemann and t. congolense broden. mean infection rates of the g. longipennis were 1.1% and 0. 55% for t. vivax and t. congolense respectively at tank e, and 0.88% and 0.15% at lali. experimen ...200011082557
tsetse and other biting fly responses to nzi traps baited with octenol, phenols and acetone.octenol (1-octen-3-ol), acetone, 4-methylphenol, 3-n-propylphenol, and other potential attractants (human urine, stable fly faeces), as well as guiacol, creosol (potential repellents), were tested as baits for biting flies in north america using standard phthalogen blue if3gm cotton nzi traps, or similar commercial polyester traps. baits were tested during the summers of 2001-04 at a residence in canada and during january-august 2001 at a dairy in the u.s.a. behaviour in the presence of octenol ...200717373949
spatial and temporal distribution of tsetse fly trap catches at nguruman, southwest kenya.spatial and temporal dynamics of rapidly growing populations of tsetse flies at nguruman, southwest kenya during 1993-1995, were investigated, following six years of intensive population suppression with traps over a c. 100 km2 area. the two tsetse species present were randomly distributed in the short rainy season, but were aggregated in the dry and long rainy seasons. maximum temperature was the dominant weather factor associated with the degree of aggregation. trends in catches at 20 fixed si ...200111415476
purification and characterization of a midgut lectin-trypsin complex from the tsetse fly glossina longipennis.a blood-meal-induced lectin (agglutinin) with proteolytic activity was isolated from midgut extracts of glossina longipennis by a two-step procedure involving anion-exchange chromatography. it is a glycoprotein [native molecular weight (m(r) 61,000 +/- 3000 da) composed of two noncovalently-linked subunits designated alpha (m(r), approximately 27,000 da) and beta (m(r), approximately 33,000 da). the trypsin activity and the glycosyl residues were present on the alpha- and beta-subunits, respecti ...19957624283
comparative susceptibility of glossina longipennis and g. brevipalpis to pathogenic species of trypanosoma. 19989622377
large-scale rearing of glossina longipennis in the laboratory. 19901980571
activity patterns in glossina longipennis: a field study using different sampling methods.studies n the daily activity of glossina longipennis at galana ranch using a black odour-baited electrocuting target confirmed its crepuscular activity profile. activity started at 05.00-05.30 hours and peaked at 06.00-06.30 hours, stopped by 09.00 hours, then started again at 17.00-17.30 hours with a peak at 18.30-19.00 hours, ceasing by 19.30 hours. females made up 60% of the overall catch, and tended to arrive later than males. other stationary sampling methods (trap, stationary ox) gave simi ...19989824824
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