| detection and differentiation of aino and akabane simbu serogroup bunyaviruses by nested polymerase chain reaction. | reverse transcription-polymerase chain reaction (rt-pcr) and nested pcr were developed to detect and differentiate aino (aino) and akabane (aka) virus s rna. two pairs of aino- and aka-specific primers for nested pcr were synthesized and examined for their capacity to amplify pcr products using 7 simbu serogroup viruses isolated in japan and australia. rt- and nested pcr using aka-specific primers amplified cdna from tinaroo virus rna as well as homologous rna. nested pcr products were different ... | 1999 | 10603165 |
| genetic diversity and reassortments among akabane virus field isolates. | sequencing and phylogenetic analysis were carried out for 35 akabane virus (akav) field isolates collected from japan, taiwan, australia and kenya, and for one tinaroo virus (tinv). of the three rna segments, the m rna segment encoding the glycoproteins that induce neutralization antibodies was the most variable among the isolates. the difference in the m rna segments among asian (japanese and taiwanese) isolates was not large (<12.3% nucleotide (nt) and <5.9% amino acid (aa) differences), rathe ... | 2007 | 17659802 |
| enhanced arbovirus surveillance with deep sequencing: identification of novel rhabdoviruses and bunyaviruses in australian mosquitoes. | viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. a metagenomics approach was used to identify virus sequences in australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. sequence comparisons revealed strains of liao ning virus (reovirus, seadornavirus), previously detected only in china, livestock-infecting stretch lagoon virus (reovirus, orbivirus), two novel dimarhabdov ... | 2013 | 24314645 |