| sensitivity and specificity of nucleic acid probes for potato leafroll luteovirus detection. | complementary dna (cdna) probes, prepared by nick-translation or by oligolabelling of a 520 bp fragment representing residues 4741 to 5261 in the potato leafroll luteovirus (plrv) sequence, were equally sensitive, with a detection limit equivalent to sap from about 600 micrograms of infected potato or nicotiana clevelandii leaf tissue and to rna from about 120 micrograms tissue. increasing the concentration of oligolabelled probe gave similar results with shorter autoradiographic exposures, but ... | 1991 | 1804851 |
| relationships among luteoviruses based on nucleic acid hybridization and serological studies. | the luteoviruses barley yellow dwarf virus (bydv-pav and bydv-rpv), bean leaf roll virus (blrv) beet western yellows virus (bwyv), carrot red leaf virus, potato leaf roll virus, and soybean dwarf virus (sdv) were compared by hybridization with random cdna probes and serologically with polyclonal antisera. for hybridizations, filters had each rna blotted in duplicate dots at 10 ng/dot. random-primed cdna probes were prepared from 300 ng of each rna and used to probe filters at three levels of str ... | 1990 | 2338398 |
| distribution of hammerhead and hammerhead-like rna motifs through the genbank. | hammerhead ribozymes previously were found in satellite rnas from plant viroids and in repetitive dna from certain species of newts and schistosomes. to determine if this catalytic rna motif has a wider distribution, we decided to scrutinize the genbank database for rnas that contain hammerhead or hammerhead-like motifs. the search shows a widespread distribution of this kind of rna motif in different sequences suggesting that they might have a more general role in rna biology. the frequency of ... | 2000 | 10899150 |
| rt-pcr using redundant primers to detect the three viruses associated with carrot motley dwarf disease. | a method was developed for detecting and distinguishing the viruses associated with carrot motley dwarf (cmd) disease, i.e. carrot mottle umbravirus (cmov), carrot red leaf virus (crlv) and the virus known as carrot red leaf virus-associated rna (crlvarna). redundant primers were made that targeted the rna-dependent rna polymerase (rdrp) gene in all available sequences of umbraviruses and in a subset of polerovirus genomes, and specific and redundant primers were made to target the same gene in ... | 2000 | 10960703 |
| the complete genome sequence, organization and affinities of carrot red leaf virus. | a sequence of 5723 nucleotides (genbank accession number: ay695933) is reported for the rna genome of an isolate of carrot red leaf virus (ctrlv). the sequence is predicted to contain six large open reading frames and non coding sequences of 28 nucleotides at the 5' end, 110 nucleotides at the 3' end, and 215 nucleotides between the two main blocks of coding sequences. the 5' coding region encodes two polypeptides with calculated molecular masses (mr) of 28.6 kda (p0) and 68.2 kda (p1) that over ... | 2005 | 15883658 |
| a small rna resembling the beet western yellows luteovirus st9-associated rna is a component of the california carrot motley dwarf complex. | abstract virions were purified from anthriscus cerefolium or coriandrum sativum plants infected with the viruses that cause california carrot motley dwarf. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of virion preparations yielded a single prominent protein species of approximately 28,000 molecular weight; however, denaturing agarose gel electrophoresis showed that virions contained three prominent single-stranded rnas of approximately 5.6, 4.2, and 2.8 kb. northern hybridization a ... | 1998 | 18944986 |
| nucleotide sequence of a satellite rna associated with carrot motley dwarf in parsley and carrot. | carrot motley dwarf (cmd) is known to result from a mixed infection by two viruses, the polerovirus carrot red leaf virus and one of the umbraviruses carrot mottle mimic virus or carrot mottle virus. some umbraviruses have been shown to be associated with small satellite (sat) rnas, but none have been reported for the latter two. a cmd-affected parsley plant was used for sap transmission to test plants, that were used for dsrna isolation. the presence of a 0.8-kbp dsrna indicated the occurrence ... | 2009 | 19020967 |
| generic detection of poleroviruses using an rt-pcr assay targeting the rdrp coding sequence. | in this study a two-step rt-pcr assay was developed for the generic detection of poleroviruses. the rdrp coding region was selected as the primers' target, since it differs significantly from that of other members in the family luteoviridae and its sequence can be more informative than other regions in the viral genome. species specific rt-pcr assays targeting the same region were also developed for the detection of the six most widespread poleroviral species (beet mild yellowing virus, beet wes ... | 2014 | 24374125 |
| carrot yellow leaf virus is associated with carrot internal necrosis. | internal necrosis of carrot has been observed in uk carrots for at least 10 years, and has been anecdotally linked to virus infection. in the 2009 growing season some growers had up to 10% of yield with these symptoms. traditional diagnostic methods are targeted towards specific pathogens. by using a metagenomic approach with high throughput sequencing technology, other, as yet unidentified causes of root necrosis were investigated. additionally a statistical analysis has shown which viruses are ... | 2014 | 25365290 |