| cloning of a cdna encoding a putative molt-inhibiting hormone from the eyestalk of the sand shrimp metapenaeus ensis. | degenerate primers were designed from the amino acid sequence of the neuropeptide pej-sgp-iv of the shrimp penaeus japonicus. reverse transcriptase-polymerase chain reaction (rt-pcr) was performed using eyestalk complementary dna of the sand shrimp metapenaeus ensis. a partial cdna that codes for a protein homologous to the neuropeptide pej-sg-iv was cloned. the partial cdna was used as a probe to screen the eyestalk cdna library. several cdna clones with nucleotide sequence identical to the par ... | 1998 | 9701616 |
| identification and molecular characterization of charybdis feriatus tropomyosin, the major crab allergen. | crab sensitivity is one of the most common seafood allergies. however, to date, there has been no report on the molecular characterization of crab allergens and no comparative analysis with other seafood allergens. | 1998 | 9819304 |
| pcr cloning and expression of the molt-inhibiting hormone gene for the crab (charybdis feriatus). | a pcr-based genomic dna walking technique was used to clone the gene for the molt-inhibiting hormone of the crab, charybdis feriatus. several overlapping genomic clones were isolated, and the mih gene for the crab was reconstructed. dna sequence determination of the overlapping clone reveals that the mih gene spans 4.3kb and consists of three exons and two introns. exons 1 and 2 carry a coding sequence for the signal peptide, and exons 2 and 3 consist of coding sequence for the mature peptide. t ... | 1998 | 9931416 |
| cloning and characterisation of mitochondrial manganese superoxide dismutase (mtmnsod) from the giant freshwater prawn macrobrachium rosenbergii. | a cdna encoding a mitochondrial manganese superoxide dismutase (mtmnsod) was cloned from the hepatopancreas of giant freshwater prawn macrobrachium rosenbergii using reverse transcription polymerase chain reaction (rt-pcr) by degenerate primers. both 3'- and 5'-regions were isolated by rapid amplification of cdna end (race) pcr method. analysis of nucleotide sequence revealed that the mtmnsod full-length cdna consists of 1202bp containing an open reading frame of 654bp, which encodes a protein c ... | 2006 | 16621606 |
| occurrence and density of vibrio parahaemolyticus in live edible crustaceans from markets in china. | vibrio parahaemolyticus is a marine bacterium causing foodborne disease. occurrence of the bacterium was investigated in six species of edible crustaceans available from markets in mainland china. the bacterium was detected in 22 of 45 whole-body, shell, and feces samples, including mitten crabs, which are supposed to be produced in freshwater ponds. the mean densities ranged from 2.8 log cfu/g in mitten crabs (eriocheir sinensis) to 5.1 cfu/g in giant tiger prawns (penaeus monodon). in hemolymp ... | 2006 | 17133820 |
| identification of the major allergens of charybdis feriatus (red crab) and its cross-reactivity with portunus pelagicus (blue crab). | tropomyosin and arginine kinase have been identified as the major allergens in multiple species of crab. charybdis feriatus is an important commercial crab in this country. | 2012 | 23393908 |
| computational analysis of molt-inhibiting hormone from selected crustaceans. | molt-inhibiting hormone (mih) is a principal endocrine hormone regulating the growth in crustaceans. in total, nine mih peptide sequences representing members of the family penaeidae (penaeus monodon, litopenaeus vannamei, marsupenaeus japonicus), portunidae (portunus trituberculatus, charybdis japonica, charybdis feriata), cambaridae (procambarus bouvieri), parastacidae (cherax quadricarinatus) and varunidae (eriocheir sinensis) were selected for our study. in order to develop a structure based ... | 2013 | 24041714 |
| [mass spectrometry identification and immune cross-reactivity of a minor shrimp allergen-sarcoplasmic calcium binding protein from litopenaeus vannamei]. | to identify sarcoplasmic calcium-binding protein (scp) as a minor shrimp allergen by mass spectrometry, and to analyze the immune cross-reactivity among crustacean scps. | 2012 | 22863586 |