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[isolation of free-living amoebae from the nasal mucosa of man. potential risk (author's transl)].from march 1976 until december 1978, we have analysed 1039 nasal swabs in order to discover the healthy free-living amoebae carriers. so, we have isolated 9 strains of which one hartmannella vermiformis. from the 8 remaining acanthamoebae, only one, orl 561 (acanthamoeba hatchetti) is as pathogenic for mice as the 2 other identical known strains and a. culbertsoni.1979120123
incubation of water samples containing amoebae improves detection of legionellae by the culture method.some protozoans isolated from aquatic habitats, including domestic water supplies, can support the intracellular replication of autochthonous legionellae in vitro. we studied the effect of incubating water samples containing amoebae on the sensitivity of culture for legionellae. samples collected during investigations of legionellosis epidemics and shown by conventional culture procedures to contain amoebae, but not legionellae, were incubated at 35 degrees c and replated. legionellae were recov ...19921622278
stimulatory effect of cooling tower biocides on amoebae.two species of amoebae were isolated from the cooling tower of an air-conditioning system and examined for effects of exposure to four cooling tower biocides, a thiocarbamate compound, tributyltin neodecanoate mixed with quaternary ammonium compounds, another quaternary ammonium compound alone, and an isothiazolin derivative. the amoebae isolated were acanthamoeba hatchetti and a cochliopodium species. two other amoeba cultures, an a. hatchetti culture and cochliopodium bilimbosum, were obtained ...19938250551
occurrence and genetic characterisation of acanthamoeba spp. from environmental and domestic water sources in queen elizabeth protected area, uganda.acanthamoeba is an emerging potentially pathogenic amoeba that has been receiving increasing attention worldwide as a reservoir and potential vector for the transmission of pathogenic bacteria. it is also associated with brain cell damage, keratitis and skin irritation in humans. its effects are more severe in immunocompromised individuals. this study provides for the first time in uganda, information on the prevalence and genotypes of acanthamoeba in environmental and domestic (tap) water.201626935431
identification and quantification of the acanthamoeba species and genotypes from reservoirs in taiwan by molecular techniques.the occurrence of acanthamoeba was investigated from 21 main reservoirs of taiwan with 12 (57.1%) testing positive. analysis of the 18s rrna gene pcr product was performed in order to identify the acanthamoeba isolates. acanthamoeba spp. concentrations were determined according to taqman real-time qpcr. acanthamoeba genotypes of all isolates were identified t4. the species were categorized to acanthamoeba culbertsoni, acanthamoeba polyphaga, acanthamoeba castellanii and acanthamoeba hatchetti. t ...201424388954
cytotoxic effect of acriflavine against clinical isolates of acanthamoeba spp.acanthamoeba keratitis (ak) is a potentially devastating and sight-threatening infection of the cornea caused by the ubiquitous free-living amoebae, acanthamoeba species. its eradication is difficult because the amoebas encyst, making it highly resistant to anti-amoebic drugs. acriflavine neutral (acf) has been used for treatment of microbial infections for humans and fishes. the aim of our study was to evaluate the time-dependent cytotoxicities of acf against acanthamoeba spp. trophozoites and ...201323052789
free-living protozoa in the gastrointestinal tract and feces of pigs: exploration of an unknown world and towards a protocol for the recovery of free-living protozoa.associations with free-living protozoa (flp) have been implicated in the persistence of foodborne pathogenic bacteria in food-related environments. to date however no information is available on the presence of flp in the gastrointestinal tract (git) of pigs, which represents an important reservoir for zoonotic foodborne bacteria and hence a potential location for associations with flp. this is at least partly due to the lack of adequate protocols to recover flp from intestinal content and feces ...201627369581
a clinical acanthamoeba isolate harboring two distinct bacterial endosymbionts.acanthamoebae feed on bacteria but are also frequent hosts of bacterial symbionts. here, we describe the stable co-occurrence of two symbionts, one affiliated to the genus parachlamydia and the other to the candidate genus paracaedibacter (alphaproteobacteria), within a clinical isolate of acanthamoeba hatchetti genotype t4. we performed fluorescence in situ hybridization (fish) and transmission electron microscopy (tem) to describe this symbiosis. our study adds to other reports of simultaneous ...201627344110
severe amoebic placentitis in a horse caused by an acanthamoeba hatchetti isolate identified using next-generation sequencing.a case of amoebic placentitis in a mare from eastern australia was diagnosed postpartum by histopathological examination of the placenta. the identity of the etiological agent was confirmed as acanthamoeba hatchetti by use of diversity profiling based on a next-generation sequencing approach.201424829227
diversity and seasonal impact of acanthamoeba species in a subtropical rivershed.this study evaluated the presence of acanthamoeba species in the puzih river watershed, which features typical subtropical monsoon climate and is located just above the tropic of cancer in taiwan. the relationship between the seasonal and geographical distributions of acanthamoeba species in this rivershed was also investigated. acanthamoeba species were detected in water samples using the amoebal enrichment culture method and confirmed by pcr. a total of 136 water samples were included in this ...201324490160
miltefosine and polyhexamethylene biguanide: a new drug combination for the treatment of acanthamoeba keratitis.in this study, a series of compounds - miltefosine, polyhexamethylene biguanide, chlorhexidine and propamidine isethionate - and combinations of the latter three agents with miltefosine were prepared and used in a rat model for the topical treatment of acanthamoeba keratitis.201423601234
adaptation of amoebae to cooling tower biocides.adaptation of amoebae to four cooling tower biocides, which included a thiocarbamate compound, tributyltin neodecanoate mixed with quaternary ammonium compounds (tbt/qac), another qac alone, and an isothiazolin derivative, was studied. previously we found that amoebae isolated from waters of cooling towers were more resistant to cooling tower biocides than amoebae from other habitats. acanthamoeba hatchetti and cochliopodium bilimbosum, obtained from american type culture collection and used in ...199424190342
efficacy of miltefosine for topical treatment of acanthamoeba keratitis in syrian hamsters.acanthamoeba keratitis is a painful corneal infection and difficult to treat because no sufficiently efficient drug has yet been available. the aim of the study therefore was to assess the therapeutic potential of miltefosine on acanthamoeba keratitis-infected hamster eyes. the cornea of hamsters were infected with acanthamoeba hatchetti, a human corneal isolate. on the fifth day, all the cornea were microscopically examined in order to determine the degree of infections (g, from 0 to 3). four g ...201121748351
[value of nu/nu mice in the production of experimental amoebic keratitis with acanthamoeba].the intracorneal inoculation of nude mice with acanthamoeba hatchetti amoebae induced a keratitis with disruption of corneal fibres, inflammatory response, secondary vascularization and amoebic trophozoites.19892516476
pathogenic amoebas from brackish and ocean sediments, with a description of acanthamoeba hatchetti, n. sp.acanthamoeba culbertsoni was isolated from a sewage-spoil dump site near ambrose light, new york bight. a second species, acanthamoeba hatchetti, n. sp., was isolated from brewerton channel, baltimore harbor, maryland. both species killed laboratory mice after infection by the intranasal route.1977867031
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