| phylogenetic diversity of the deinococci as determined by 16s ribosomal dna sequence comparison. | 16s ribosomal dna (rdna) sequences were determined for the five species of the genus deinococcus (deinococcus erythromyxa, deinococcus proteolyticus, deinococcus radiodurans, deinococcus radiophilus, and deinococcus radiopugnans) and the single species of the genus. deinobacter (deinobacter grandis). with the exception of deinococcus erythromyxa, the deinococci form a coherent phylogenetic cluster which is related to the thermus-meiothermus lineage. an analysis of the 16s rdna sequence of deinoc ... | 1997 | 9103641 |
| meiothermus cerbereus sp. nov., a new slightly thermophilic species with high levels of 3-hydroxy fatty acids. | strains of meiothermus cerbereus sp. nov. were isolated from the hot springs within the geysir geothermal area of iceland. the strains of meiothermus cerbereus produce red-orange-pigmented colonies, have an optimum growth temperature of about 55 degrees c, and have higher levels of 3-oh fatty acids than the strains of the other species of the genus meiothermus. these strains, unlike all other strains of the species of the genus meiothermus examined previously, required cysteine, thiosulfate, or ... | 1997 | 9336933 |
| f-and v-atpases in the genus thermus and related species. | the discovery of a v-type atpase in the gram-negative bacterium thermus thermophilus hb8 (yokoyama et al., j. biol. chem. 265, 21946, 1990) was unexpected, since only eukaryotic endomembranes and archaea were thought to contain this enzyme complex, and horizontal gene transfer was suggested to explain the finding. we examined membrane-associated atpases from representatives of several groups of the genus thermus. the enzymes were extracted with chloroform and purified by ion exchange chromatogra ... | 1998 | 9741106 |
| characterization of glycolipids from meiothermus spp. | thin-layer chromatographic analysis of the polar lipids of meiothermus strains revealed two glycolipid bands with similar chromatographic mobility to the major glycolipid of thermus strains. in this study the glycolipids from the type strains of meiothermus ruber, meiothermus chliarophilus, meiothermus silvanus and meiothermus cerbereus were characterized using gc, gc/ms, fast atom bombardment ms and chemical methods. all strains contained dihexosyl-(n-acyl)hexosaminylglucosyl diacylglycerols, r ... | 1999 | 10376835 |
| carotenoid glycoside esters from the thermophilic bacterium meiothermusruber | the thermophilic bacterium meiothermus ruber produces a series of carotenoid glycoside esters. the major carotenoid has been identified as 1'-beta-glucopyranosyl-3,4,3',4'-tetradehydro-1', 2'-dihydro-beta,psi-caroten-2-one (1). it is acylated at the 6' '-position of the glucose unit by a series of c10-c17 fatty acids. the structure of 1 was established by spectral means, including complete assignment of the 1h and 13c nmr resonances by inverse 2d nmr spectroscopy. these carotenoids are thought t ... | 1999 | 10395503 |
| horizontal transfer of archaeal genes into the deinococcaceae: detection by molecular and computer-based approaches. | members of the deinococcaceae (e.g., thermus, meiothermus, deinococcus) contain a/v-atpases typically found in archaea or eukaryotes which were probably acquired by horizontal gene transfer. two methods were used to quantify the extent to which archaeal or eukaryotic genes have been acquired by this lineage. screening of a meiothermus ruber library with probes made against thermoplasma acidophilum dna yielded a number of clones which hybridized more strongly than background. one of these contain ... | 2000 | 11116332 |
| [unique properties of highly radioresistant bacteria]. | in connection with the chernobyl nuclear power plant (chnpp) accident and the negative ecological after-effects for biota in this zone the interest has arisen to radioresistant bacteria, as to the most dynamic model of the given ecosystem, and to mechanisms which provide resistance of bacteria to ionizing radiation. the analysis of published data has shown that the radioresistant bacteria are not interrelated taxonomically and phylogenetically. the extreme radioresistant bacteria are represented ... | 2000 | 11300085 |
| meiothermus taiwanensis sp. nov., a novel filamentous, thermophilic species isolated in taiwan. | two novel filamentous bacterial isolates, strains wr-30t and wr-220, with an optimum growth temperature of approximately 55-60 degrees c were isolated from wu-rai hot springs in the northern part of taiwan. these isolates were aerobic, thermophilic, non-sporulating, red-pigmented and heterotrophic and formed extremely long, filamentous trichomes from cells of different lengths. phylogenetic analysis of 16s rdna, dna-dna hybridization, morphological and biochemical features and fatty acid composi ... | 2002 | 12361269 |
| meiothermus rosaceus sp nov isolated from tengchong hot spring in yunnan, china. | a rosy-pigmented gram-negative, thermophilic bacterium with an optimum growth temperature of about 55 degrees c was isolated from tengchong hot springs in yunnan province, china. its growth scarcely occurred below 40 degrees c or above 70 degrees c. phylogenetic and secondary structural analyses of 16s rrna and dna-dna hybridization showed that the organism represented a new species of the genus meiothermus. this new species could be distinguished easily from other species of the genus meiotherm ... | 2002 | 12435512 |
| colored moderately thermophilic bacteria in paper-machine biofilms. | biofilms cause several problems in papermaking. this report describes a microbiological survey of colored biofilms in six paper and board machines, including two case studies of outbreaks of colored slimes in which the causative bacteria were found. a total of 95 pink-, red-, orange- or yellow-pigmented strains were isolated. nearly all (99%) of the strains grew at 52 degrees c, 72% grew at 56 degrees c, but only 30% grew at 28 degrees c, indicating that most of the strains were moderately therm ... | 2003 | 12700951 |
| identification and characterization of a hsp70 (dnak) chaperone system from meiothermus ruber. | we have cloned the genes encoding the chaperones of meiothermus ruber, hsp70 (mru.hsp70), hsp40 (mru.hsp40) and hsp22 (mru.hsp22). the genes hsp70, hsp22 and hsp40 of m. ruber are organized into an operon. the amino acid sequences of the three m. ruber chaperones show strong similarity with the heat shock proteins of thermus thermophilus. both mru.hsp40 and its homolog from t. thermophilus lack a cysteine-rich region. however, recombinant mru.hsp70 and mru.hsp40 associate in an atp-dependent man ... | 2003 | 12715159 |
| cloning of an alkaline phosphatase gene from the moderately thermophilic bacterium meiothermus ruber and characterization of the recombinant enzyme. | a gene that codes for an alkaline phosphatase was cloned from the thermophilic bacterium meiothermus ruber, and its nucleotide sequence was determined. the deduced amino acid sequence indicates that the enzyme precursor including the putative signal sequence is composed of 503 amino acid residues and has an estimated molecular mass of 54,229 da. comparison of the peptide sequence with that of the prototype alkaline phosphatase from escherichia coli revealed conservation of the regions in the vic ... | 2003 | 12928867 |
| [thermostability of alkaline phosphatase of meithermus ruber bacteria: cloning of the gene, expression in escherichia coli cells and biochemical characterization of the recombinant protein]. | the meiothermus ruber alkaline phosphatase gene was cloned, expressed in escherichia coli cells, and sequenced. the enzyme precursor, including the putative signal peptide, was shown to consist of 503 residues (deduced molecular mass 54,229 da). the recombinant enzyme showed the maximal activity at 60-65 degrees c and ph 11.0 and had k(m) = 0.055 mm as estimated with p-nitrophenyl phosphate (pnpp). the enzyme proved to be moderately thermostable, retaining 50% activity after 6 h incubation at 60 ... | 2003 | 14714494 |
| distinctive protein signatures provide molecular markers and evidence for the monophyletic nature of the deinococcus-thermus phylum. | the deinococcus-thermus group of species is currently recognized as a distinct phylum solely on the basis of their branching in 16s rrna trees. no unique biochemical or molecular characteristics that can distinguish this group from all other bacteria are known at present. in this work, we describe eight conserved indels (viz., inserts or deletions) in seven widely distributed proteins that are distinctive characteristics of the deinococcus-thermus phylum but are not found in any other group of b ... | 2004 | 15126471 |
| structural determination of the polar glycoglycerolipids from thermophilic bacteria meiothermus taiwanensis. | the polar glycolipids were isolated from the thermophilic bacteria meiothermus taiwanensis atcc baa-400 by ethanol extraction and purified by sephadex lh-20 and silica gel column chromatography. the fatty acid composition of o-acyl groups in the glycolipids was obtained by gas chromatography mass spectroscopy analysis on their methyl esters derived from methanolysis and was made mainly of c(15:0) (34.0%) and c(17:0) (42.3%) fatty acids, with the majority as branched fatty acids (over 80%). remov ... | 2004 | 15560795 |
| identification of meiothermus as the dominant genus in a storage system for spent nuclear fuel. | to characterize the biofilms in an interim wet storage system (clab) for spent nuclear fuel. | 2005 | 15715877 |
| polyamine analysis for chemotaxonomy of thermophilic eubacteria: polyamine distribution profiles within the orders aquificales, thermotogales, thermodesulfobacteriales, thermales, thermoanaerobacteriales, clostridiales and bacillales. | cellular polyamines of 45 thermophilic and 8 related mesophilic eubacteria were investigated by hplc and gc analyses for the thermophilic and chemotaxonomic significance of polyamine distribution profiles. spermidine and a quaternary branched penta-amine, n4-bis(aminopropyl)norspermidine, were the major polyamine in thermocrinis, hydrogenobacter, hydrogenobaculum, aquifex, persephonella, sulfurihydrogenibium, hydrogenothermus, balnearium and thermovibrio, located in the order aquificales. thermo ... | 2004 | 15747232 |
| meiothermus timidus sp. nov., a new slightly thermophilic yellow-pigmented species. | several yellow-pigmented isolates, with optimum growth temperatures between 55 and 60 degrees c, were recovered from hot springs in central portugal and the azores. phylogenetic analysis of the 16s rdna showed that these organisms represented a new species of the genus meiothermus. the new isolates could be distinguished from other strains of the species of the genus meiothermus by biochemical characteristics and the fatty acid composition because they had very high levels of iso c15:0 and iso c ... | 2005 | 15796977 |
| distribution of f- and a/v-type atpases in thermus scotoductus and other closely related species. | the presence of an a/v-type atpase in different thermus species and in the deeper branching species meiothermus ruber and deinococcus radiodurans suggests that the presence of the archaeal-type atpase is a primitive character of the deinococci that was acquired through horizontal gene transfer (hgt). however, the presence of a bacterial type f-atpases was reported in two newly identified thermus species (thermus scotoductus dsm 8553 and thermus filiformis dsm 4687). two different scenarios can e ... | 2006 | 16423651 |
| structural elucidation of phosphoglycolipids from strains of the bacterial thermophiles thermus and meiothermus. | the structures of two major phosphoglycolipids from the thermophilic bacteria thermus oshimai ntu-063, thermus thermophilus ntu-077, meiothermus ruber ntu-124, and meiothermus taiwanensis ntu-220 were determined using spectroscopic and chemical analyses to be 2'-o-(1,2-diacyl-sn-glycero-3-phospho) -3'-o-(alpha-n-acetyl-glucosaminyl)-n-glyceroyl alkylamine [pgl1 (1)] and the novel structure 2'-o-(2-acylalkyldio-1-o-phospho)-3'-o-(alpha-n-acetylglucosaminyl)-n-glyceroyl alkylamine [pgl2 (2)]. pgl2 ... | 2006 | 16675854 |
| detection and quantitation of colored deposit-forming meiothermus spp. in paper industry processes and end products. | colored biofilms cause problems in paper industry. in this work we used real-time pcr to detect and to quantitate members of the genus meiothermus from the process samples and end products from 24 machines manufacturing pulp, paper and board in four countries. the results obtained from 200 samples showed the importance of members of the genus meiothermus as ubiquitous biofoulers in paper machines. this genus was the dominant biofouler in some mills. from < or =10(4) to 10(11) copies of meiotherm ... | 2007 | 17131103 |
| synthesis of a tetrasaccharide glycosyl glycerol. precursor to glycolipids of meiothermus taiwanensis atcc baa-400. | synthesis of a tetrasaccharide glycosyl glycerol, the core structure of glycoglycerolipid from meiothermus taiwanensis atcc baa-400, was described. a one-pot glycosylation with three components was employed as a key step. | 2007 | 17579454 |
| tlr-independent induction of human monocyte il-1 by phosphoglycolipids from thermophilic bacteria. | the structures of phosphoglycolipids pgl1 and pgl2 from the thermophilic bacteria meiothermus taiwanensis, meiothermus ruber, thermus thermophilus, and thermus oshimai are determined recently (yang et al. in j lipid res. 47:1823-1932, 2006). these bacteria belong to gram-negative bacteria that do not contain lipopolysaccharide, but high amounts of phosphoglycolipids and glycoglycerolipids. here we show that pgl1/pgl2 mixture (pgl1: pgl2 = 10:1 ~ 10:2) from m. taiwanensis and t. oshimai, but not ... | 2008 | 18161025 |
| electrochemical inactivation of paper mill bacteria with mixed metal oxide electrode. | in this study electrochemical inactivation of selected bacteria living in paper mill circulating waters was investigated. three aerobic bacteria species (deinococcus geothermalis, pseudoxanthomonas taiwanensis and meiothermus silvanus) were inactivated effectively (>2 log) at a mixed metal oxide (mmo) electrode in 3 min. the influence of parameters, such as current density and initial ph or chloride concentration of synthetic paper machine water (spw) on the inactivation efficiency were studied. ... | 2008 | 18206301 |
| microbe repelling coated stainless steel analysed by field emission scanning electron microscopy and physicochemical methods. | coating of stainless steel with diamond-like carbon or certain fluoropolymers reduced or almost eliminated adhesion and biofilm growth of staphylococcus epidermidis, deinococcus geothermalis, meiothermus silvanus and pseudoxanthomonas taiwanensis. these species are known to be pertinent biofilm formers on medical implants or in the wet-end of paper machines. field emission scanning electron microscopic analysis showed that staph. epidermidis, d. geothermalis and m. silvanus grew on stainless ste ... | 2008 | 18379832 |
| isolation and identification of a thermophilic strain producing trehalose synthase from geothermal water in china. | a slightly thermophilic strain, cbs-01, producing trehalose synthase (tres), was isolated from geothermal water in this study. according to the phenotypic characteristics and phylogenetic analysis of the 16s rrna gene sequence, it was identified as meiothermus ruber. the trehalose synthase gene of meiothermus ruber cbs-01 was cloned by polymerase chain reaction and sequenced. the tres gene consisted of 2,895 nucleotides, which specified a 964-amino-acid protein. this novel tres catalyzed reversi ... | 2008 | 18685210 |
| quantitative contributions of bacteria and of deinococcus geothermalis to deposits and slimes in paper industry. | deinococcus geothermalis has frequently been isolated from pink colored deposits of paper industry processes. laboratory studies have shown that d. geothermalis is capable of forming on nonliving surfaces patchy biofilms that are resistant to adverse agents such as extreme ph, desiccation, solubilising detergents and biocides. this study was done to quantitatively assess the role of d. geothermalis as a biofouler in paper industry. colored deposits were collected from 24 european and north ameri ... | 2008 | 18726625 |
| fatty acids, unusual glycophospholipids and dna analyses of thermophilic bacteria isolated from hot springs. | the composition of fatty acids in 12 strains of the genera thermus, meiothermus, geobacillus and alicyclobacillus was analyzed by gas chromatography-mass spectrometry. major fas found in the profiles included i-15:0, i-17:0, ai-15:0, i-16:0, 16:0, ai-17:0, together with some minor components. branched fas were predominant, forming more than 80% of all fas measured. fast atom bombardment-mass spectrometry was used for analysis of unusual glycophospholipids, i.e., acylglycosylcardiolipins from gen ... | 2009 | 18989616 |
| sustainable and practical degradation of intact chicken feathers by cultivating a newly isolated thermophilic meiothermus ruber h328. | the sustainable and practical degradation of intact chicken feathers by a newly isolated thermophilic bacterium meiothermus ruber h328 was presented with extensive data. aerobic cultivation with moderately thermophilic strain h328 at 55 degrees c for 6 days led to the apparently complete decay of the truly intact feathers and provided 1.89 mmol free amino acids and 7.32 mmol acid-hydrolyzed amino acids from 50 ml of culture containing 3% (w/v) intact chicken feathers. the amino acid components i ... | 2009 | 19194700 |
| meiothermus rufus sp. nov., a new slightly thermophilic red-pigmented species and emended description of the genus meiothermus. | four red-pigmented isolates, with optimum growth temperatures of approximately 55-60 degrees c and an optimum ph for growth between 7.5 and 8.5, were recovered from hot springs in central france. phylogenetic analysis of the 16s rrna gene sequences showed that these organisms represented a new species of the genus meiothermus. the new isolates could be distinguished from other strains of the species of the genus meiothermus primarily by the glycolipid profile and fatty acid composition because t ... | 2009 | 19577874 |
| [cloning, expression and functional analysis of the genes in tps/tpp trehalose synthetic pathway of meiothermus ruber]. | by constructing the genomic dna library of meiothermus ruber cbs-01, the genes of trehalose phosphate synthase (tps) and trehalose phosphate phosphatase (tpp) involved in trehalose synthesis were cloned. the genes were cloned into the plasmid pet21a, and expressed in escherichia coli rosetta gami (de3). the activities of these two purified enzymes were confirmed by thin layer chromatography (tlc). meanwhile, we tested the cellular compatible solutes of m. ruber cbs-01 under different environment ... | 2009 | 19621581 |
| meiothermus cateniformans sp. nov., a slightly thermophilic species from north-eastern china. | two aerobic, non-motile and non-sporulating bacteria, strains ly1( t) and l462, were recovered from a hot spring located in the qianshan area of north-eastern china. the novel strains had gram-negative cell walls and grew at 30-66 degrees c (optimum 55-60 degrees c) and ph 5.5-10.0 (optimum ph 8.0-8.5). 16s rrna gene sequence similarity analysis revealed that strains ly1( t) and l462 were most closely related to meiothermus ruber atcc 35948(t), m. taiwanensis wr-30(t) and m. cerbereus gy-1(t), w ... | 2010 | 19661497 |
| the phylogenetic diversity of thermus and meiothermus from microbial mats of an australian subsurface aquifer runoff channel. | spectral analysis of the cell free extracts of four mat samples colonizing a great artesian bain (gab) aquifer bore runoff channel suggested that thermus was present in the 75 degrees c grey mat, meiothermus was present in the 66 degrees c red mat, a mixed population of meiothermus/thermus and photosynthetic microbes were present in the 57 degrees c green mat and photosynthetic microbes were present in the 52 degrees c brown mat. enumeration studies indicated that thermus dominated the grey mats ... | 2004 | 19712377 |
| amplified ribosomal dna restriction analysis of free-living bacteria present in the headbox of a canadian paper machine. | the headbox water is the main source of bacterial contamination of paper machines. identification of these bacterial contaminants could be an asset in developing specific control methods. an amplified ribosomal dna restriction analysis (ardra) was carried out to characterize the bacterial communities associated with the headbox water of a paper machine in a canadian mill in february and july 2006. eight bacterial genera were identified as the main colonizers present in the headbox water. the gen ... | 2009 | 19767853 |
| overexpression and characterization of a thermostable trehalose synthase from meiothermus ruber. | a thermostable trehalose synthase (tres) gene from meiothermus ruber cbs-01 was cloned and overexpressed in escherichia coli. the purified recombinant tres could utilize maltose to produce trehalose, and showed an optimum ph and temperature of 6.5 and 50 degrees c, respectively. kinetic analysis showed that the enzyme had a twofold higher catalytic efficiency (k (cat)/k (m)) for maltose than for trehalose, indicating maltose as the preferred substrate. the tres also had a weak hydrolytic propert ... | 2010 | 19779856 |
| decomposition of intact chicken feathers by a thermophile in combination with an acidulocomposting garbage-treatment process. | in order to develop a practical method for the decomposition of intact chicken feathers, a moderate thermophile strain, meiothermus ruber h328, having strong keratinolytic activity, was used in a bio-type garbage-treatment machine working with an acidulocomposting process. the addition of strain h328 cells (15 g) combined with acidulocomposting in the garbage machine resulted in 70% degradation of intact chicken feathers (30 g) within 14 d. this degradation efficiency is comparable to a previous ... | 2009 | 19897897 |
| a unique group of virus-related, genome-integrating elements found solely in the bacterial family thermaceae and the archaeal family halobacteriaceae. | viruses sh1 and p23-77, infecting archaeal haloarcula species and bacterial thermus species, respectively, were recently designated to form a novel viral lineage. in this study, the lineage is expanded to archaeal halomicrobium and bacterial meiothermus species by analysis of five genome-integrated elements that share the core genes with these viruses. | 2010 | 20400546 |
| meiothermus granaticius sp. nov., a new slightly thermophilic red-pigmented species from the azores. | two red-pigmented isolates, with optimum growth temperatures between 45 and 50 degrees c, were recovered from a hot spring in the furnas, area da fonte 1825 on the island of são miguel in the azores. phylogenetic analysis of the 16s rrna gene sequences showed that these organisms represented a new species of the genus meiothermus. these new isolates could be distinguished from other strains of the species of the genus meiothermus primarily by the fatty acid composition and polar lipid pattern, s ... | 2010 | 20451341 |
| structural variation of glycolipids from meiothermus taiwanensis atcc baa-400 under different growth temperatures. | a major glycolipid, alpha-galf(1-3)-alpha-galp(1-6)-beta-glcpnacyl(1-2)-alpha-glcp(1-1)-2-acylalkyldiol, is obtained from meiothermus taiwanensis. this novel glycolipid is found only when the bacterium grows above 62 degrees c, which is significantly different from those from the same bacteria incubated at 55 degrees c. terminal galactofuranoside and 1,2-alkyldiol lipids replaced galactopyranoside and glycerol lipids, respectively, under increased growth temperature. this variation is likely nec ... | 2010 | 20668750 |
| biofilm-forming bacteria with varying tolerance to peracetic acid from a paper machine. | biofilms cause runnability problems in paper machines and are therefore controlled with biocides. peracetic acid is usually effective in preventing bulky biofilms. this study investigated the microbiological status of a paper machine where low concentrations (≤15 ppm active ingredient) of peracetic acid had been used for several years. the paper machine contained a low amount of biofilms. biofilm-forming bacteria from this environment were isolated and characterized by 16s rrna gene sequencing, ... | 2010 | 21161323 |
| isolation and characterization of a new bacteriophage mmp17 from meiothermus. | thermophiles and their viruses are extraordinarily important because of their roles in processes of evolution, biogeochemistry and ecology. species of the genus meiothermus share close relationship with genus thermus, but no meiothermus bacteriophage has been reported yet. in this work, a new thermophilic bacteriophage named mmp17 (meiothermus myoviridae phage 17) was isolated from a meiothermus strain and was further characterized. mmp17 was a typical myovirus with an icosahedral head (42 nm in ... | 2011 | 21225300 |
| complete genome sequence of meiothermus ruber type strain (21). | meiothermus ruber (loginova et al. 1984) nobre et al. 1996 is the type species of the genus meiothermus. this thermophilic genus is of special interest, as its members share relatively low degrees of 16s rrna gene sequence similarity and constitute a separate evolutionary lineage from members of the genus thermus, from which they can generally be distinguished by their slightly lower temperature optima. the temperature related split is in accordance with the chemotaxonomic feature of the polar l ... | 2010 | 21304689 |
| complete genome sequence of meiothermus silvanus type strain (vi-r2). | meiothermus silvanus (tenreiro et al. 1995) nobre et al. 1996 belongs to a thermophilic genus whose members share relatively low degrees of 16s rrna gene sequence similarity. meiothermus constitutes an evolutionary lineage separate from members of the genus thermus, from which they can generally be distinguished by their slightly lower temperature optima. m. silvanus is of special interest as it causes colored biofilms in the paper making industry and may thus be of economic importance as a biof ... | 2010 | 21304690 |
| composition of the bacterial biota in slime developed in two machines at a canadian paper mill. | during the process of papermaking by pulp and paper plants, a thick and viscous deposits, termed slime, is quickly formed around the paper machines, which can affect the papermaking process. in this study, we explored the composition of the bacterial biota in slime that developed on shower pipes from 2 machines at a canadian paper mill. firstly, the composition was assessed for 12 months by dna profiling with polymerase chain reaction coupled with denaturing gradient gel electrophoresis. except ... | 2011 | 21326351 |
| meiothermus hypogaeus sp. nov., a novel moderately thermophilic bacterium isolated from a hot spring. | a novel moderately thermophilic, red-pigmented bacterium, designated strain azm34c11(t), was isolated from the bottom of a 1000 meter deep drilled well located in a japanese hot spring. the isolated cells were gram-negative and grew optimally at 50 °c, ph 7.6, and 0-0.3 % (w/v) nacl concentration. analysis of the 16s rrna gene revealed that the isolate belonged to the genus meiothermus, and the sequence similarities between the isolate and other meiothermus species were 88.2-94.8 %. strain azm34 ... | 2011 | 21335495 |
| biosynthesis of ω-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria geobacillus stearothermophilus and meiothermus ruber. | two thermophilic strains belonging to geobacillus stearothermophilus and meiothermus ruber, which naturally do not synthesize ω-alicyclic fatty acids (ω-fas) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize ω-fas when cultivated with the appropriate precursor. the incorporation of cyclic acids influenced the whole fa com ... | 2011 | 21487936 |
| crystal structure of the cytoplasmic n-terminal domain of subunit i, a homolog of subunit a, of v-atpase. | subunit "a" is associated with the membrane-bound (v(0)) complex of eukaryotic vacuolar h(+)-atpase acidification machinery. it has also been shown recently to be involved in diverse membrane fusion/secretory functions independent of acidification. here, we report the crystal structure of the n-terminal cytosolic domain from the meiothermus ruber subunit "i" homolog of subunit a. the structure is composed of a curved long central a-helix bundle capped on both ends by two lobes with similar a/ß a ... | 2011 | 21787787 |
| sequence of the hyperplastic genome of the naturally competent thermus scotoductus sa-01. | many strains of thermus have been isolated from hot environments around the world. thermus scotoductus sa-01 was isolated from fissure water collected 3.2 km below surface in a south african gold mine. the isolate is capable of dissimilatory iron reduction, growth with oxygen and nitrate as terminal electron acceptors and the ability to reduce a variety of metal ions, including gold, chromate and uranium, was demonstrated. the genomes from two different thermus thermophilus strains have been com ... | 2011 | 22115438 |
| rhamnolipid-producing thermophilic bacteria of species thermus and meiothermus. | novel rhamnolipid-producing strains of three thermophilic bacteria, thermus sp., t. aquaticus and meiothermus ruber were identified that have not been previously described as rhamnolipid producers. rhamnolipids were extracted from supernatant and further purified by thin-layer chromatography. mass spectrometry with negative electrospray ionization revealed 77 rhamnolipid homologues varying in chain length and unsaturation. tandem mass spectrometry identified mono-rhamnolipid and di-rhamnolipid h ... | 2011 | 21984420 |
| characterization of mspni (g/gwcc) and mspnii (r/gatcy), novel thermostable type ii restriction endonucleases from meiothermus sp., isoschizomers of avaii and bstyi. | mspni and mspnii, isoschizomers of prototype type ii restriction endonucleases avaii and bstyi, were extracted from an extreme thermophile bacterium belonging to the genus meiothermus, isolated from the hot sulphur springs in north himalayan region of india where temperature and ph ranged from 60 to 80°c and 7.5 to 8.5, respectively. the two enzymes were purified to homogeneity using cibacron-blue 3ga agarose, q-sepharose and sp-sepharose chromatography and were homodimers with subunit molecular ... | 2011 | 22189541 |