| polar glycosphingolipids in insect: chemical structures of glycosphingolipid series containing 2'-aminoethylphosphoryl-(----6)-n-acetylglucosamine as a polar group from larvae of the green-bottle fly, lucilia caesar. | a series of glycosphingolipids containing 2'-aminoethylphosphoryl(----6)-n-acetylglucosamine as a polar group has been demonstrated in larvae of the green-bottle fly, lucilia caesar. the thin-layer chromatographic pattern of the total polar glycolipid revealed the presence of more than eight components, of which five major components were purified by the use of successive column chromatography on qae- and deae-sephadex and silicic acid (iatrobeads). from structural studies including compositiona ... | 1991 | 1778972 |
| studies on glycosphingolipids in larvae of the green-bottle fly, lucilia caesar: two neutral glycosphingolipids having large straight oligosaccharide chains with eight and nine sugars. | two neutral glycosphingolipids having large straight oligosaccharide chains with eight and nine sugars, provisionally named cos and cns, were isolated and purified from larvae of the green-bottle fly, lucilia caesar, as the only two remaining unidentified significant neutral glycolipids in this organism. from the results of sugar analysis, permethylation, negative-ion fast atom bombardment mass spectroscopy (fab-ms), and 1h-nmr studies, the structures of the two glycolipids are proposed to be: c ... | 1990 | 2391351 |
| [asthma caused by lucilia caesar larvae: clinical and immunologic study]. | lucilia caesar larvae (lcl) are used as live fish bait by anglers. five cases of asthma and rhinoconjunctivitis following exposure to lcl are reported. three had work-related asthma as they were working on a fish bait farm or shop and two had asthma when they went fishing. in one subject exposure to lcl caused asthma, rhinoconjunctivitis and contact urticaria. in four subjects peak expiratory flow rate (pefr) was monitored during exposure to lcl. in three out of four subjects there was evidence ... | 1989 | 2630895 |
| characterization of two glucuronic acid-containing glycosphingolipids in larvae of the green-bottle fly, lucilia caesar. | two glucuronic acid-containing glycosphingolipids were purified from larvae of the green-bottle fly, lucilia caesar by deae-sephadex and iatrobeads column chromatography. structures of these acidic glycolipids, glycolipids x and y, were elucidated by means of sugar analysis, permethylation, enzymatic hydrolysis, negative-ion fast atom bombardment mass spectrometry, and nmr studies. glycolipid x was determined to have the following structure: glca beta 1-3gal beta 1-3galnac alpha 1-4 galnac beta ... | 1989 | 2768250 |
| [wound myiasis in cattle infested by lucilia caesar (diptera: calliphoridae)]. | a case report on facultative wound myiasis after sectio caesarea in a cow by larvae of lucilia caesar in may 1986. | 1987 | 3445964 |
| [wound infaction in a dog caused by the blowfly lucilia caesar (linnaeus, 1758) (diptera: calliphoridae)]. | | 1971 | 4931781 |
| variations in the photoperiodic reaction controlling diapause induction in lucilia caesar l. (diptera: calliphoridae). | | 1971 | 5546098 |
| isolation of sphingoethanolamine from pupae of the green-bottle fly, lucilia caesar. | | 1968 | 5707810 |
| studies on glycosphingolipids of larvae of the green-bottle fly, lucilia caesar. i. isolation and characterization of glycosphingolipids having novel sugar sequences. | the neutral glycosphingolipids from larvae of the green-bottle fly, lucilia caesar, were analyzed. thin-layer chromatograms showed that the larvae contained at least seven major glycolipid components. of them, the four components with shorter sugar chains consisting of one to four sugar units, were purified by iatrobeads column chromatography. the structures were identified by partial acid hydrolysis, sequential enzymatic hydrolysis, chromium trioxide oxidation and methylation analysis as: glc b ... | 1982 | 7130143 |
| studies on glycosphingolipids of larvae of the green-bottle fly, lucilia caesar. ii. isolation and structural studies of three glycosphingolipids with novel sugar sequences. | three novel glycosphingolipids containing five to seven sugars in their saccharide chains have been isolated from larvae of the green-bottle fly, lucilia caesar. the structures of these glycolipids were identified by sequential enzymatic degradation with exoglycosidases, methylation analysis, and partial acid hydrolysis to be: gal-nac alpha (1-4)galnac beta (1-4)glcnac beta (1-3)man beta (1-4)glc-cer, gal alpha (1-4)galnac alpha (1-4)galnac beta (1-4)glcnac beta (1-3)man beta (1-4)glc-cer, and g ... | 1982 | 7142125 |
| asthma caused by live fish bait. | larvae of insects and worms are commonly used as live fish bait (lfb) by anglers. asthma, rhinoconjunctivitis, and urticaria related to various kinds of lfb have been reported. | 1994 | 8120269 |
| the evolution of ectoparasitism in the genus lucilia (diptera:calliphoridae). | to consider the evolutionary origin of the ectoparasitic habit in the blowfly genus lucilia (diptera:calliphoridae), phylogenetic analyses of mitochondrial dna sequence data were performed for 10 species, including all the common lucilia agents of myiasis, collected from africa, australasia, north america and europe. complementary genetic distance and parsimony analyses are used to consider inter and intraspecific relationships within the genus with reference to previous morphological work. the ... | 1997 | 9076529 |
| seasonal and spatial changes in blowfly production from small and large carcasses at durham in lowland northeast england. | colonization by blowflies (diptera: calliphoridae) of mouse carcasses exposed in open agricultural land near durham (54 45'n) changed from early spring monopolization by calliphora vicina r.-d. to a summer pattern of multiple species exploitation by this species together with lucilia caesar l., l. illustris mg., l. silvarum mg., l. sericata mg. and l. richardsi collin. in a garden at the edge of durham, mouse carcasses were dominated by c. vicina from spring to autumn. difference in mouse coloni ... | 1999 | 10514049 |
| partial sequencing of the cytochrome oxydase b subunit gene i: a tool for the identification of european species of blow flies for postmortem interval estimation. | the identification of insects found on a dead body can lead to the estimation of the time of death (postmortem interval). we report an updated version of an established method based on sequence analysis of pcr products from a region of the cytochrome b oxidase subunit i mitochondrial gene of different members of the family calliphoridae, by sequencing six european species: lucilia sericata (meigen), lucilia caesar (linné), lucilia illustris (meigen), calliphora vicina (robineau-desvody), calliph ... | 2000 | 10914577 |
| diptera as vectors of mycobacterial infections in cattle and pigs. | mycobacteria were isolated from 14 (4.5%) of 314 samples, containing 7791 adult diptera, which were collected in the czech republic and slovakia in 1997-2000. these flies were collected from three cattle herds with paratuberculosis, two pig herds with mycobacterial infections and one farm that kept both cattle and pigs and that did not have problems of mycobacterial infections. mycobacterium intracellulare was isolated from eristalis tenax linnaeus (diptera: syrphidae) captured from a pig herd. ... | 2001 | 11434556 |
| synthetic studies on glycosphingolipids from the protostomia phyla: syntheses of arthro-series glycosphingolipids. | glycosphingolipids isolated from larvae of the green-bottle fly, lucilia caesar, have quite unique structures containing glcnacbeta-(1 --> 3)-man and galnacbeta-(1 --> 4)-glcnacbeta-(1 --> 3)-man. we have synthesized two glycosphingolipids, beta-d-glcnacp-(1 --> 3)-beta-d-manp-(1 --> 4)-beta-d-glcp-(1 --> 1)-cer and beta-d-galnacp-(1 --> 4)-beta-d-glcnacp-(1 --> 3)-beta-d-manp-(1 --> 4)-beta-d-glcp-(1 --> 1)-cer. a key reaction in the synthetic sequence is the application of the intramolecular a ... | 2002 | 12433469 |
| neurosecretory cells in the brain of the larva of lucilia caesar l. | | 1957 | 13407695 |
| differences in the ecology of lucilia caesar (l.) and lucilia illustris (meig.) (diptera, callphoridae) in finland. | | 1964 | 14348753 |
| blowflies calliphora vicina and lucilia sericata as passive vectors of mycobacterium avium subsp. avium, m. a. paratuberculosis and m. a. hominissuis. | mycobacterium avium subsp. paratuberculosis (actinomycetales: mycobacteriaceae) isolates of identical restriction fragment length polymorphism (rflp) type b-c1 were isolated from: intestinal mucosa of two cows showing clinical signs of paratuberculosis, a specimen of the blowfly calliphora vicina robineau-desvoidy (diptera: calliphoridae) captured while perched on these cattle intestines in a waste container at the site of the slaughter, and the blowflies c. vicina and lucilia caesar linnaeus ca ... | 2004 | 15189236 |
| carriage of bacteria by proboscises, legs, and feces of two species of flies in street food vending sites in ouagadougou, burkina faso. | flies are widely recognized as potential reservoirs and vectors of bacteria. in the present study, an attempt was made to assess meat-poultry and local fruit juice processing and vending sites for their hygienic status and the presence of houseflies, musca domestica, and blow flies, lucilia caesar, for bacterial carriage. the hygienic status results revealed the presence of waste and sewage nearby which provided food and harborage for flies. on the two sites, the m. domestica population was domi ... | 2006 | 16924933 |
| use of lucilia species for forensic investigations in southern europe. | the aim of this study was to highlight the importance of evaluating entomological evidence in forensic investigations on a regional scale. to evaluate climatic, geographical and environmental influences on the selection of carrion-breeding fauna in northern italy and consequently on inferred forensic data (post-mortem intervals and post-mortem transfer), we present details of six indoor-outdoor cases. results show that the most abundant species was lucilia sericata, together with other fly speci ... | 2008 | 18079080 |
| myiasis of the tracheostomy wound: case report. | "myiasis" is a parasitic infestation of live human or vertebrate animal tissues or cavities caused by dipterous larvae (maggots) which feed on the host's dead or living tissue, liquid body substances or ingested food. they are extremely rare in western countries, especially in e.n.t. practice, and to the best of our knowledge, only two cases of myiasis in a tracheostomy wound have been reported in the english literature. the case is reported, probably the first, of percutaneous tracheotomy myias ... | 2006 | 18236640 |
| responses of tabanidae (diptera) to canopy traps baited with 4-methylphenol, 3-isopropylphenol, and naphthalene. | the attraction of female tabanids to unbaited and single-baited canopy traps using 4-methylphenol, 3-isopropylphenol, and naphthalene was studied in three forest localities in eastern croatia. tabanids were collected in a significantly higher number in traps baited with these chemicals compared to unbaited control traps. the number of females of tabanus bromius, tabanus sudeticus, tabanus tergestinus, hybomitra ciureai, haematopota pluvialis, and tabanus maculicornis collected from 4-methylpheno ... | 2007 | 18260506 |
| terminal contact elements of insect attachment devices studied by transmission x-ray microscopy. | for the first time, the terminal elements (spatulae) of setal (hairy) attachment devices of the beetle gastrophysa viridula (coleoptera, chrysomelidae) and the fly lucilia caesar (diptera, calliphoridae) were studied using transmission x-ray microscopy (txm) with a lateral resolution of about 30 nm. since images are taken under ambient conditions, we demonstrate here that this method can be applied to study the contact behaviour of biological systems, including animal tenent setae, in a fresh st ... | 2008 | 18515726 |
| influxed insects as vectors for campylobacter jejuni and campylobacter coli in danish broiler houses. | the vector potential of flies (diptera: brachycera) for spread of campylobacter jejuni and campylobacter coli on 5 danish broiler farms was evaluated in a longitudinal field study from april to november 2004. first, the prevalence of c. jejuni- and c. coli-positive flies was determined in 2,816 flies captured from farm surroundings. each individual fly was macerated, preenriched in bolton broth for 24 h at 42 degrees c, streaked onto modified campylobater blood-free selective agar and incubated ... | 2008 | 18577626 |
| an initial study of insect succession and carrion decomposition in various forest habitats of central europe. | at the end of summer and beginning of fall 2005 insect succession and pig carrion decomposition were studied in pine-oak forest, hornbeam-oak forest, and alder forest in western poland (central europe). five stages of decomposition were recognized: fresh stage, bloated stage, active decay stage, advanced decay stage, and remains stage. the highest rate of decomposition was observed in the alder forest and the lowest in the pine-oak forest. a similar composition of carrion entomofauna was found i ... | 2008 | 18715728 |
| molecular identification of forensically important blowfly species (diptera: calliphoridae) from germany. | forensic entomology applies knowledge about the behaviour and ecology of insects associated to corpses to homicide investigations. it is possible to calculate a minimum post-mortem interval by determining the age of the oldest blowfly larvae feeding on a corpse. the growth rate of the larvae is highly dependent on temperature and also varies between the different blowfly species infesting a corpse. it is, thus, crucial to correctly identify the species collected from a crime scene. to increase t ... | 2009 | 19862555 |
| use of cytochrome c oxidase subunit i (coi) nucleotide sequences for identification of the korean luciliinae fly species (diptera: calliphoridae) in forensic investigations. | blowflies, especially species belonging to the subfamily luciliinae, are the first insects to lay eggs on corpses in korea. fast and accurate species identification has been a key task for forensic entomologists. because conventional morphologic identification methods have many limitations with respect to forensic practice, molecular methods have been proposed to identify fly species of forensic importance. to this end, the authors amplified and sequenced the full length of the cytochrome c oxid ... | 2009 | 19949660 |
| [how many neuroblasts build mushroom bodies in lucilia caesar l. and musca domestica l. (diptera, brachycera cyclorrhapha)?]. | the green-bottle fly lucilia caesar and the housefly musca domestica differ greatly in the number of neuroblasts producing mushroom bodies. four neuroblasts were found in each mushroom body of lucilia pupae, and its calyx has a quadruple structure. in the housefly, the number of mushroom body neuroblasts rises up 20 in each brain hemisphere. this leads to a more complicated calyx structure. the neuroblast number observed in lucilia and musca is compared with that found in other diptera. | 2009 | 20146414 |
| [sequencing of mitochondrial dna cytochrome oxidase subunit i gene in sarcosaphagous flies from 14 provinces in china]. | to detect the 278 bp region of gene of the cytochrome oxidase subunit i (coi) in mitochondral dna (mtdna) of sarcosaphagous flies, identify the species of sarcosaphagous flies, and provide reference for forensic application. | 2010 | 20818074 |
| the use of coi barcodes for molecular identification of forensically important fly species in germany. | deoxyribonucleic acid (dna)-based insect identification has become a routine and accurate tool in forensic entomology. in the present study, we demonstrate the utility of the mitochondrial dna cytochrome oxidase i gene "barcoding region" as a universal marker for molecular identification of forensically important diptera. we analyzed 111 specimens belonging to 13 species originating from frankfurt am main, germany (calliphoridae: calliphora vicina, calliphora vomitoria, lucilia ampullacea, lucil ... | 2011 | 22186975 |
| outbreak of type c botulism in birds and mammals in the emilia romagna region, northern italy. | over a 7-day period beginning 8 august 2011, a large number of wild birds of several species were found dead or with neurologic clinical signs along the shore of crostolo stream, in the emilia romagna region, italy. twenty-eight mallards (anas platyrhynchos), two hooded crows (corvus corone cornix), and three coypus (myocastor coypus) were found moribund on the crostolo stream bank, collected, and sent to istituto zooprofilattico sperimentale della lombardia e dell'emilia romagna, reggio emilia ... | 2013 | 24502738 |
| synanthropy and temporal variability of calliphoridae living in cosenza (calabria, southern italy). | the aim of this study was to investigate the synanthropy of diptera: calliphoridae, insects of forensic importance, in calabria (southern italy). the study lasted 2 years, from february 2010 to january 2012, and was carried out in three areas of cosenza province representing "urban", "rural", and "wild" environments. bottle traps baited with pork were used to catch calliphoridae. six species were identified, calliphora vicina robineau-desvoidy 1830, calliphora vomitoria (l.), chrysomya albiceps ... | 2014 | 25527571 |
| applicability of partial characterization of cytochrome oxidase i in identification of forensically important flies (diptera) from china and egypt. | precise species identification of every insect sample collected from criminal scenes play an essential role in the accurate estimation of postmortem interval. the morphological similarity poses a great challenge for forensic entomologists. dna-based method can be used as a supplemental means of morphological method. in the present study, we demonstrate the applicability of the 304-bp cytochrome oxidase i gene fragment in molecular identification of forensically important diptera. we analyzed 75 ... | 2013 | 23661266 |
| cytochrome b as a useful tool for the identification of blowflies of forensic interest (diptera, calliphoridae). | in forensic entomology the fast and accurate identification of insects collected at the scene of events is essential if errors are to be avoided in estimating infestation times of interest and determining the post-mortem interval (pmi). traditional identification based on morphological characteristics can be complicated due to physical similarities between different species, especially at immature stages. genetic analysis provides a fast and reliable identification method. in this paper, molecul ... | 2013 | 23597749 |
| carrion flies of forensic interest: a study of seasonal community composition and succession in lisbon, portugal. | information on diptera community, seasonality and successional patterns in every geographical region is fundamental for the use of flies as forensic indicators of time of death. in order to obtain these data from the lisbon area (portugal), experiments were conducted during the four seasons of the year, using piglet carcasses as animal models. five stages were recognized during the decomposition process. the stages, besides visually defined, could be separated taking into account the occurrence ... | 2012 | 22765479 |
| using the developmental gene bicoid to identify species of forensically important blowflies (diptera: calliphoridae). | identifying species of insects used to estimate postmortem interval (pmi) is a major subject in forensic entomology. because forensic insect specimens are morphologically uniform and are obtained at various developmental stages, dna markers are greatly needed. to develop new autosomal dna markers to identify species, partial genomic sequences of the bicoid (bcd) genes, containing the homeobox and its flanking sequences, from 12 blowfly species (aldrichina grahami, calliphora vicina, calliphora l ... | 2013 | 23586044 |
| species composition of forensically important blow flies (diptera: calliphoridae) and flesh flies (diptera: sarcophagidae) through space and time. | weekly monitoring of forensically important flight-active blow flies (diptera: calliphoridae) and flesh flies (diptera: sarcophagidae) was performed using small baited traps. sampling took place in two rural, one suburban and two urban habitats in and around frankfurt (main), germany, lasting two years and eight months. highest values for species richness and chao-shen entropy estimator for shannon's index in both families were found at the urban sites, peaking during summer. space-time interact ... | 2014 | 24529768 |
| temperature-dependent appearance of forensically useful flies on carcasses. | flies are frequently used for postmortem interval (pmi) estimations. these estimates are usually based on the age of larval or pupal specimens. however, the age defines only the minimum pmi. in order to move forensic entomology further, a method useful for the estimation of an interval preceding insect appearance on a corpse called the pre-appearance interval (pai) is needed. recently, it was demonstrated that the pai of several carrion beetles is closely related to the temperature prevailing th ... | 2014 | 24096988 |
| muscle attachment site (mas) patterns for species determination in european species of lucilia (diptera: calliphoridae). | species identification is generally assessed to be more difficult in larval stages than in adult forms. especially closely related species such as lucilia caesar and lucilia illustris are difficult to identify. the aim of this study was to simplify species determination in lucilia larvae for entomological and forensic purposes. muscle attachment site (mas) patterns were previously found to be a good tool for species determination in blowfly larvae. here, distinctive mas patterns are presented fo ... | 2015 | 25500717 |
| morphology and identification of first instars of the european and mediterranean blowflies of forensic importance. part ii. luciliinae. | first instars of lucilia ampullacea villeneuve, lucilia caesar linnaeus, lucilia cuprina weidemann, lucilia richardsi collin, lucilia sericata meigen and lucilia silvarum meigen (diptera: calliphoridae) are thoroughly documented with scanning electron microscopy images, light microscopy photographs and line drawings. the following morphological structures are documented: pseudocephalon, antennal complex, maxillary palpus, facial mask, cephaloskeleton, thoracic and abdominal spinulation, spiracul ... | 2013 | 23205742 |
| molecular identification of forensically important calliphoridae and sarcophagidae species using its2 nucleotide sequences. | the application of insect evidence to forensic investigations is mainly based on the estimation of postmortem interval and the identification of insect species from samples that are collected from the crime scene. due to the limited number of expert taxonomists, species identification is one of the major barriers for crime scene investigators to utilize forensic entomology. therefore, the molecular identification of species, using mitochondrial cytochrome c oxidase subunit i (coi) gene, has been ... | 2018 | 29331679 |
| oleic acid is a precursor of linoleic acid and the male sex pheromone in nasonia vitripennis. | linoleic acid (c18:2(δ9,12), la) is crucial for many cell functions in organisms. it has long been a paradigm that animals are unable to synthesize la from oleic acid (c18:1(δ9), oa) because they were thought to miss δ(12)-desaturases for inserting a double bound at the δ(12)-position. today it is clear that this is not true for all animals because some insects and other invertebrates have been demonstrated to synthesize la. however, the ability to synthesize la is known in only five insect orde ... | 2014 | 24874439 |
| unusual pseudomyiasis with musca domestica (housefly) larvae in a tracheostomy wound: a case report and literature review. | we report a case of pseudomyiasis (accidental myiasis) in a 52-year-old man who had previously undergone surgery and placement of a tracheostomy tube for laryngeal cancer. the patient presented to our outpatient department with a foul-smelling, blood-stained discharge and frequent and severe episodic pain at the site of the tracheostomy. he also reported the discharge of many whitish larvae from the site. on clinical examination, 3 of these larvae were removed from the tracheostomy wound and lat ... | 2013 | 23904316 |
| why is the molecular identification of the forensically important blowfly species lucilia caesar and l. illustris (family calliphoridae) so problematic? | species of the fly genus lucilia are commonly used in forensic investigations to estimate the postmortem interval (pmi). two close-related species lucilia caesar and l. illustris are difficult to identify. previous studies showed that the mitochondrial cytochrome c oxidase subunit i (coi) marker could be used to identify many lucilia species. however, mixed results were obtained for l. caesar and l. illustris due to some european specimens showing identical haplotypes. here, we investigated 58 n ... | 2012 | 22964163 |