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[biology of triatoma vitticeps (stal, 1859) under laboratory conditions (hemiptera: reduviidae: triatominae). ii. resistance to fasting].following the study on the biology of triatoma vitticeps (gonçalves et al., 1988) observations have been made on its resistance to starvation. of the 286 eggs obtained only 201 hatched and reached the intended stage for observation. the others did not eclode, neither reached the ecdisis nor died, without explanation. the nymphs were kept, separately, in borrel flasks and properly listed. the blood-meal was performed in mice, although the insects were kept without feeding as soon as moulted. the ...19892181243
[biology of triatoma vitticeps (stal, 1859) under laboratory conditions (hemiptera: reduviidae: triatominae). i. evolutive cycle].observations were made on the evolutive cycle of triatoma vitticeps, held under laboratory conditions and fed weekly in mice. of the 435 eggs obtained, from 4 virgin couples, 149 were purposed for the biological cycle study and 286 to evaluate their resistance to starvation, which shall be a second part of this work. only 50 specimens reached the adult stage in a period of means (s) = 270 +/- 45 days. at the incubation time, the first and second instars were of less than a month for each, while ...19883078351
attempts to improve xenodiagnosis: comparative test of sensibility using rhodnius neglectus, panstrongylus megistus, triatoma vitticeps and triatoma infestans in endemic areas of brazil.from june 1984 to july 1992, 392 xenodiagnostic tests were applied on 264 patients with chronic chagas disease from brazilian endemic areas of virgem da lapa and coronel murta, situated in the jequitinhonha valley, in the state of minas gerais. the susceptibilities of rhodnius neglectus, panstrongylus megistus, triatoma vitticeps and triatoma infestans were compared. most of the time 20 nymphs (fourth instar) of each species were applied to 161 women and 103 men aged between 5 and 83 years of ag ...19979302418
dynamics of evolution and resistance to starvation of triatoma vitticeps (stal 1859) (reduviidae: triatominae), submitted to two different regimens of food deprivation.the resistance to starvation of triatoma vitticeps has been analyzed comparatively, according to different regimens of food deprivation under laboratory conditions. one cohort, composed of 100 specimens of each evolutionary nymphal stage, was submitted to continuous fasting until death; the second group, arranged in the same way, was fed once on chicken. through this work, it was possible not only to compare the results obtained from the first group with other authors' results but, also, to anal ...200212471435
[natural infection of triatoma vitticeps (stal, 1859) with flagellates morphologically similar to trypanosoma cruzi (chagas, 1909) in espírito santo state].adult specimens of triatoma vitticeps are frequently captured by residents in rural areas of the state of espírito santo, brazil. with aim of determining the natural infection rate of this species, we examined the excrement of 116 wild specimens, captured in 27 municipal districts of the state, after blood meal in chicken and spontaneous excretion. of these, 100 (86.2%) were infected with flagellates morphologically similar to trypanosoma cruzi. our results showed natural infection rates of trit ...200616501776
peridomiciliary colonies of triatoma vitticeps (stal, 1859) (hemiptera, reduviidae, triatominae) infected with trypanosoma cruzi in rural areas of the state of espírito santo, brazil.in brazil, the colonization of human dwellings by triatomines occurs in areas with native vegetation of the caatinga or cerrado types. in areas of atlantic forest such as in the brazilian state of espírito santo, there are no species adapted to live in human habitations. the few autochthonous cases of chagas disease encountered in espírito santo have been attributed to adult specimens of triatoma vitticeps that invade houses from forest remnants. in recent years, the entomology unit of the espír ...200516184222
feeding patterns of triatoma vitticeps in the state of rio de janeiro, brazil.feeding patterns of triatomines have contributed to elucidate its biology. triatoma vitticeps, naturally infected with t. cruzi, has been found in domiciles. its behavior and epidemiological patterns were investigated.200010973153
an investigation on the ecology of triatoma vitticeps (stal, 1859) and its possible role in the transmission of trypanosoma cruzi, in the locality of triunfo, santa maria madalena municipal district, state of rio de janeiro, brazil.from january 1989 to april 1995, 465 specimens of triatoma vitticeps were collected in the locality of triunfo, 2nd district of santa maria madalena municipal district, state of rio de janeiro. the bugs were found indoors by local residents with predominance of adults. the flight activity was high in hot months when the incidence in the domicile also increased. two hundred and two bugs (111 alive and 91 dead) were examined for trypanosoma cruzi infection. this was detected in 31 of the dead bugs ...19989921289
the effect of azadirachtin on fresh isolates of trypanosoma cruzi in different species of triatomines.the effect of azadirachtin was investigated using three different fresh isolates of trypanosoma cruzi and five different triatomine species which were infected as third-instar larvae. the two t. cruzi strains which originated from sylvatic triatoma vitticeps showed a high prevalence after the molt to the fifth instar in panstrongylus megistus and rhodnius neglectus and a low prevalence in triatoma infestans and rhodnius robustus. the third t. cruzi strain originating from a patient in piaui show ...19989569093
effects of the spore-endotoxin complex of a strain of bacillus thuringiensis serovar morrisoni upon triatoma vitticeps (hemiptera: reduviidae) under laboratory conditions. 19947476224
[geographic distribution of triatoma vitticeps stal, 1859 (hemiptera, reduviidae) in the state of rio de janeiro, brazil]. 19863077191
lethality of triatomines (hemiptera: reduviidae), vectors of chagas' disease, feeding on blood baits containing synthetic insecticides, under laboratory conditions.a laboratory study was conducted to test the toxicity of synthetic insecticides added to defibrinated sheep blood kept at room temperature and offered as food to the following triatomine species: triatoma infestans, panstrongylus megistus, triatoma vitticeps, triatoma pseudomaculata, triatoma brasiliensis and rhodnius prolixus. the insecticides used, at a concentration of 1 g/l, were: hch, ddt, malathion and trichlorfon, and the lethalithy observed at the end of a 7-day period varied according t ...19911726952
triatoma vitticeps subcomplex (hemiptera, reduviidae, triatominae): a new grouping of chagas disease vectors from south america.triatomines have been grouped into complexes and subcomplexes based largely on morphological and geographical distribution. although these groupings are not formally recognised as taxonomic ranks, they are likely monophyletic. however, recent studies have demonstrated that some subcomplexes from south america did not form monophyletic groups, and reorganisations have been suggested. one suggested reorganisation is to exclude triatoma vitticeps, t. melanocephala, and t. tibiamaculata from the t. ...201728407782
characterization of the external female genitalia of six species of triatominae (hemiptera: reduviidade) by scanning electron microscopy.by macroscopic and microscopic dorsal side observation, it was noted that the ix and x segments of two species each of panstrongylus and triatoma terminate in an elongated way, whereas they terminate abruptly in the two species of rhodnius. scanning observation of the dorsal, ventral, lateral and posterior sides of the female genitalia of panstrongylus herreri, panstrongylus megistus, rhodnius colombiensis, rhodnius prolixus, triatoma infestans and triatoma vitticeps revealed that these six spec ...201020512241
spermatogenesis in triatoma melanocephala (hemiptera: triatominae).triatoma melanocephala is a rare species of hemiptera. it belonged to the brasiliensis subcomplex and presents morphological characteristics very close to those of triatoma vitticeps. we investigated spermatogenesis of t. melanocephala and compared it with that of t. vitticeps in order to determine whether these organisms have similar cytogenetic characteristics. lacto-acetic orcein staining was used to observe all stages of meiosis. these two species were found to have the same karyotype (2n = ...201324301754
evaluation of the chagas disease control program in açucena municipality, rio doce valley, state of minas gerais, brazil.açucena municipality, rio doce valley, state of minas gerais, brazil temporarily (2001-2005) interrupted epidemiological surveillance for chagas disease. the objective of this work was to evaluate the chagas disease control program (cdcp) in açucena and to offer suggestions for improving local epidemiological surveillance.201424861292
autochthonous transmission of chagas disease in rio de janeiro state, brazil: a clinical and eco-epidemiological study.after the control of the main modes of chagas disease (cd) transmission in most endemic countries, it is important to identify the participation of native sylvatic vectors in cd transmission. although cd is not considered endemic in rio de janeiro state (rj), brazil, we identified patients with cd born in rj and investigated the possible autochthonous transmission in the state.201525566786
ecological scenario and trypanosoma cruzi dtu characterization of a fatal acute chagas disease case transmitted orally (espírito santo state, brazil).trypanosoma cruzi infection via oral route results in outbreaks or cases of acute chagas disease (acd) in different brazilian regions and poses a novel epidemiological scenario. in the espírito santo state (southeastern brazil), a fatal case of a patient with acd led us to investigate the enzootic scenario to avoid the development of new cases. at the studied locality, triatoma vitticeps exhibited high t. cruzi infection rates and frequently invaded residences.201627580853
feeding behavior of triatoma vitticeps (reduviidae: triatominae) in the state of minas gerais, brazil.the objective of this study was to evaluate the feeding behavior of triatoma vitticeps through the identification of its food sources and the characterization of the blood ingestion process. in addition, we aimed to verify if the saliva of this vector interferes with the perception of the host during the feedings by creating a nervous impulse. here, we demonstrated that the t. vitticeps saliva reduces, gradually and irreversibly, the amplitude of the compound action potential of the nervous fibr ...201121340350
morphometry and morphology of the antennae of panstrongylus megistus burmeister, rhodnius neglectus lent, rhodnius prolixus stal and triatoma vitticeps stal (hemiptera: reduviidae).the length of the four right antennal segments from nymphs and adults of panstrongylus megistus burmeister, rhodnius neglectus lent, rhodnius prolixus stal and triatoma vitticeps stal were measured. the length of the antennal segments of the adults of all four species, 4th and 5th instars of p. megistus, and 5th instar of r. neglectus and r. prolixus followed the same pattern: 2nd>3rd>4th>1st. the pattern of 1st and 2nd instars of p. megistus was: 4th>3rd>2nd>1st. for 3rd instars of p. megistus, ...201020498958
analysis of the geographical distribution of triatoma vitticeps (stål, 1859) based on data of species occurrence in minas gerais, brazil.the purpose of this study was to determine the potential for infestation of man-made environments by triatoma vitticeps, correlating the epidemiological importance of this species with that of others present in the brazilian state of minas gerais. in addition, climatic variables that could help explain the distribution of this species and clarify its population dynamics were sought. this was done by carrying out a historical survey of the presence of t. vitticeps in artificial ecotopes, using da ...201020493970
digestive cells in the midgut of triatoma vitticeps (stal, 1859) in different starvation periods.triatoma vitticeps (stal, 1859) is a hematophagous hemiptera that, although being considered wild, can be found in households, being a potential chagas' disease vector. this work describes the histology and ultrastructure of the midgut of t. vitticeps under different starvation periods. fifteen adults of both sexes starved for 3, 7, 20 and 25 days were studied. in general, digestive cells had apical microvilli, basal plasma membrane infoldings and central nucleus. the perimicrovillar membrane wa ...201020451882
major cysteine protease (cruzipain) in z3 sylvatic isolates of trypanosoma cruzi from rio de janeiro, brazil.trypanosoma cruzi, the etiologic agent of chagas' disease, is represented by a set of parasites which circulate between man, vectors, domestic and wild animals. recently, our group isolated from triatoma vitticeps strains of t. cruzi that were characterized as belonging to the z3 phylogenetic lineage. since very little is known about the biological and/or biochemical markers of sylvatic z3 isolates, we have studied the protein and protease profiles of distinct z3 isolates designated as smm10, sm ...200919437041
[focus of chagas disease in são fidélis, in the state of rio de janeiro].four specimens of triatoma vitticeps and one of panstrongylus geniculatus were caught inside a home in the municipality of são fidélis, rio de janeiro. one of the specimens of triatoma vitticeps proved positive for infection with trypanosoma cruzi. two people living in that home (one adult and one adolescent) presented positive serology.200818853020
molecular characterization of trypanosoma cruzi sylvatic isolates from rio de janeiro, brazil.trypanosoma cruzi, the etiologic agent of chagas disease, presents considerable heterogeneity between isolated populations within the wild and domestic cycles. by using multiplex polymerase chain reaction based on the mini-exon gene, characterization was performed on seven samples isolated from specimens of triatoma vitticeps that had been collected from the locality of triunfo in the municipality of santa maria madalena, state of rio de janeiro, brazil. the samples smm10, smm53, smm88, and smm9 ...200818622628
population dynamics of triatoma vitticeps (stål, 1859) in itanhomi, minas gerais, brazil.taxonomic markers (head structure morphometry, isoenzymes and randon amplified polymorphism of dna - rapd) were used to understand the population dynamics of triatoma vitticeps, predominant triatomine species in itanhomi district, using samples obtained from domestic, peridomiciliary and sylvatic habitats. morphometric analysis revealed sexual dimorphism within the three samples although specimens could not be separated according to the habitat in which they were captured. forty-two bands were a ...200818368232
dynamics of feeding and defecation in triatoma vitticeps (stal, 1859) (hemiptera, reduviidae, triatominae) and its potential in the transmission of trypanosoma cruzi.adults of triatoma vitticeps infected by flagellates similar to trypanosoma cruzi are frequently captured by the inhabitants of rural areas in the brazilian state of espirito santo. the dynamics of feeding and defecation were observed in three groups of adult triatomines, consisting of sylvatic t. vitticeps and laboratory-reared specimens of this species and t. infestans. triatomines were observed from the moment they were presented with an immobilized chicken as a bloodmeal source until 240 min ...200617072459
[source of chagas disease in arcádia, state of rio de janeiro, brazil].following the report of triatomine nymphs in a house in arcadia, miguel pereira, state of rio de janeiro, brazil, the infested dwelling was checked. several eggs and 46 specimens of triatoma vitticeps (stal, 1859) were collected. among them, adults and nymphal instars accounted for 43.5% and 56.5%, respectively. analysis of blood meals showed the ecletism of this species; 24 (52.2%) were single feeds, 18 insects (39.1%) fed on two hosts and 4 (8.7%) on three hosts. trypanosoma cruzi infection ra ...200414762512
meiosis aspects and nucleolar activity in triatoma vitticeps (triatominae, heteroptera).some aspects of both the nucleolar organizer activity and meiosis were studied in the testes of triatoma vitticeps (heteroptera, triatominae). the techniques used included squashing followed by lacto-acetic orcein staining, silver-ion impregnation, fluorescent banding (cma3, quinacrine mustard and dapi) and fluorescent in situ hybridization (fish). a close relationship between heterochromatin and nucleolus in testicular cells was observed. during meiosis, the silver-ion impregnation pattern vari ...200616502091
endocrine and regenerative cells in the midgut of chagas' disease vector triatoma vitticeps during different starvation periods.the midgut of an insect has three main cell types, viz., digestive, endocrine and regenerative cells, and a relationship between cell morphology and blood meal has been suggested. this study evaluated the occurrence of the regenerative and endocrine cells in different regions of the midgut of female and male triatoma vitticeps during different periods after a blood meal. adults of both sexes were dissected at 4, 72, 120, 168, 288, 360 and 600 hours after feeding and the midgut was pulled apart. ...201425403079
occurrence and distribution of triatomines (hemiptera: reduviidae) in municipalities of the northeastern region of minas gerais state, brazil.triatomines are targeted for the control of chagas disease in endemic areas of brazil.201727598640
trypanosoma (herpetosoma) rangeli tejera, 1920: study of the effects of the parasite on the vector.for evaluation of the possible pathogenicity of trypanosoma (herpetosoma) rangeli tejera, 1920 to the triatomid vector, first-stage nymphs of laboratory-bred insects were engorged upon albino mice showing average parasitemias of 2 x 10(6) and 2 x 10(5) trypanosomes/ml blood. the vector strains were: rhodnius prolixus ("new" strain), triatoma pallidipennis, and triatoma vitticeps. an "old" strain of r. prolixus (maintained 30 years in the laboratory) was also employed to check the effects of labo ...19892701258
high trypanosoma spp. diversity is maintained by bats and triatomines in espírito santo state, brazil.the aim of this study was to reevaluate the ecology of an area in the atlantic forest, southeast brazil, where chagas disease (cd) has been found to occur. in a previous study, immediately after the occurrence of a cd case, we did not observe any sylvatic small mammals or dogs with trypanosoma cruzi cruzi infections, but triatoma vitticeps presented high t. c. cruzi infection rates. in this study, we investigated bats together with non-volant mammals, dogs, and triatomines to explore other possi ...201729176770
spatial distribution of triatomines in domiciles of an urban area of the brazilian southeast region.reports of triatomine infestation in urban areas have increased. we analysed the spatial distribution of infestation by triatomines in the urban area of diamantina, in the state of minas gerais, brazil. triatomines were obtained by community-based entomological surveillance. spatial patterns of infestation were analysed by ripley's k function and kernel density estimator. normalised difference vegetation index (ndvi) and land cover derived from satellite imagery were compared between infested an ...201626814643
multiple mitochondrial genes of some sylvatic brazilian triatoma: non-monophyly of the t. brasiliensis subcomplex and the need for a generic revision in the triatomini.multiple fragments of mitochondrial dna genes (cytochrome b, cytochrome oxidase i, and 16s rdna) were used to evaluate the phylogenetic relationships among triatoma melanocephala, triatoma tibiamaculata, triatoma vitticeps, and other members of triatoma brasiliensis subcomplex under a bayesian framework and maximum parsimony criterion. with the addition of new sequences of t. tibiamaculata and t. vitticeps, triatoma juazeirensis, triatoma melanica and the newly sequenced t. melanocephala, the th ...201424508245
distribution of constitutive heterochromatin in species of triatomines with fragmentation of sex chromosomes x.cytogenetic analyses of triatomines are considered to be important taxonomic tools. thus, we analyzed the pattern of constitutive heterochromatin in 7 species of triatomine with fragmentation of the sex chromosome x, focusing on the cytotaxonomy of these triatomines. the species analyzed included triatoma vitticeps, triatoma melanocephala, triatoma tibiamaculata, triatoma protracta, meccus pallidipennis, panstrongylus megistus, and panstrongylus lignarius. the seminiferous tubules of the adult m ...201425501239
feeding behavior of different species of chagas' disease vectors stimulated with blood sources at different temperatures.1. the feeding behavior of six triatomid species toward latex condoms filled with blood at 26 +/- 1 and 36 +/- 1 degrees c was observed for 4 h. 2. the species studied were triatoma infestans, triatoma brasiliensis, triatoma vitticeps, triatoma pseudomaculata, rhodnius prolixus and panstrongylus megistus, and 34 to 348 insects were studied in each group (average, 190). all the life stages of each species were used. 3. although most insects (80%, average for 6 species) preferred the warmer blood, ...19921304939
[correlation among the positivity of the artificial xenodiagnosis and the amount of blood and triatomines used in the exam, in chronic chagasic patients].the aim of this study was to verify whether the amount of blood and number of triotomines used could improve the artificial xenodiagnosis performed in 200 chronic phase infected individuals. ten or 40ml of peripheral blood was collected in heparinized (20.4 iu) vacuum tubes, and fed to 60 and 360 triatomines, respectively. dipetalogaster maximus (1st instar, about 15 days after eclosion), as well as 3rd instar triatoma infestans and triatoma vitticeps and the 4th instar of rhodnius neglectus wer ...200611873258
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