| eperythrozoon wenyoni--a blood parasite of cattle. a first report in new zealand. | | 1977 | 275683 |
| letter: eperythrozoon wenyoni, a possible cause of anaemia in british cattle. | | 1976 | 936451 |
| eperythrozoon wenyoni anaemia in cattle. | | 1976 | 1014301 |
| development of a recombinant anaplasma marginale dna probe. | an anaplasma marginale dna probe has been developed by using an improved method for the isolation of genomic dna. purified genomic a. marginale dna from the st. croix isolate was partially digested with sau 3a1 into fragments (greater than or equal to 5.0 kb). the restriction fragments were cloned using standard techniques in the pbr322 vector and used to transform e. coli (dh5) host cells. the recombinant a. marginale dna library was screened by the colony lifting procedure. colonies containing ... | 1990 | 2260276 |
| enzyme-linked immunosorbent assay for detection of antibodies to eperythrozoon wenyoni in cattle. | | 1990 | 2287138 |
| eperythrozoon wenyonii infection in dairy cattle. | approximately 10 of 100 young heifers that had recently delivered their first calf--members of a large colorado dairy herd--had a syndrome of swollen teats and distal portions of the hind limbs, prefemoral lymphadenopathy, transient fever, rough coat, decreased milk production, and subsequent weight loss and reproductive inefficiency. acute clinical signs of disease were associated with large numbers of eperythrozoon wenyonii seen on blood smears, and resolution of signs correlated with reductio ... | 1990 | 2332369 |
| enzyme-linked immunosorbent assay using solubilised antigen for detection of antibodies to anaplasma marginale. | an enzyme-linked immunosorbent assay (elisa) was used to detect antibodies against anaplasma marginale. a marginale bodies were separated from parasitised erythrocytes by a modified nitrogen decompression method, sonicated, then solubilised with triton x-100 and used as the elisa antigen. in this elisa system the required amount of antigen protein was 16.2 ng for each well. in the course of experimental infections, of calves, significant antibody levels were detected by elisa and the complement ... | 1988 | 3238774 |
| anaplasma marginale, eperythrozoon wenyoni: lectin reactions with bovine erythrocytes. | normal bovine erythrocytes were agglutinated with four of five lectins specific for different oligosaccharides. the order of reactivity was wheat germ greater than ricin greater than soybean greater than peanut. concanavalin a did not agglutinate normal bovine erythrocytes. after neuraminidase treatment of normal bovine erythrocytes, each lectin agglutinated the cells with decreased concentrations of lectin, verifying that partial removal of sialic acid exposes more of each lectin's binding site ... | 1986 | 3943586 |
| eperythrozoon wenyoni: a scanning electron microscope study. | | 1973 | 4126714 |
| the infectivity of theileria mutans, eperythrozoon wenyoni and e. teganodes to sheep and of e. ovis to cattle. | | 1971 | 5003201 |
| hemoparasitism, humoral immunodeficiency, and an igg1 fragment in a cow. | an adult cow with nonregenerative anemia was found to be infected with eperythrozoon wenyoni and trypanosoma theileri. further laboratory testing revealed hypoalbuminemia, hypogammaglobulinemia, decreased numbers of circulating b lymphocytes, and presence of a serum immunoglobulin g1 fragment. reduced b-lymphocyte regions in lymphoid tissue, evidence of chronic nephritis, and infection with fasciola hepatica and sarcocystis spp were found at necropsy. the cause of the acquired humoral immunodefi ... | 1982 | 7130011 |
| use of in vitro culture to isolate babesia bovis from theileria buffeli, eperythrozoon wenyoni and anaplasma spp. | on nine separate occasions, babesia bovis microaerophilus stationary phase (masp) cultures were initiated with blood from calves with concurrent infections of b. bovis and theileria buffeli, eperythrozoon wenyoni or anaplasma spp. in each case b. bovis became established in culture and was maintained for 30-49 days. culture material was inoculated into susceptible splenectomised calves to test for persistence of the other organisms. no haemoparasites other than b. bovis were detected in giemsa-s ... | 1994 | 8091617 |
| infertility associated with eperythrozoon wenyonii infection in a bull. | a 16-month-old charolais bull was examined because of acute onset of scrotal and hind limb edema, fever, tachycardia, tachypnea, anorexia, and lethargy. scrotal circumference on initial examination was 48 cm. clinicopathologic abnormalities included microcytic, normochromic anemia and numerous eperythrozoon organisms in blood smears. results of immunohistochemical staining of a skin biopsy specimen suggested that the edema was the result of an arthus-type reaction. semen quality deteriorated rap ... | 1994 | 8144388 |
| theileria sergenti and t. buffeli: polymerase chain reaction-based marker system for differentiating the parasite species from infected cattle blood and infected tick salivary gland. | benign theileria species in cattle. theileria sergenti and t. buffeli, are morphologically indistinguishable. the polymerase chain reaction (pcr) was used to amplify the genes encoding the 33- and 34-kda major piroplasm antigens (p33/34) of t. sergenti and t. buffeli from cattle blood infected with these parasites and tick salivary gland infected with t. sergenti. following amplification, the p33 gene from t. sergenti and the p34 gene from t. buffeli were clearly differentiated using the restric ... | 1995 | 8542983 |
| the cell wall-less rickettsia eperythrozoon wenyonii is a mycoplasma. | the 16s rrna gene of eperythrozoon (haemobartonella) wenyonii, a wall-less hemotrophic prokaryote currently classified as a rickettsia, was sequenced to determine the relationship of this organism to other wall-less prokaryotes. comparison to the genbank data base showed that this hemotrophic organism is a mycoplasma (family mollicutes). phylogenetic analysis of 16s rrna genes indicated that this and other recently sequenced 16s rrna genes of hemotrophic bacteria form a new, separate branch whic ... | 1997 | 9513278 |
| proposal to transfer some members of the genera haemobartonella and eperythrozoon to the genus mycoplasma with descriptions of 'candidatus mycoplasma haemofelis', 'candidatus mycoplasma haemomuris', 'candidatus mycoplasma haemosuis' and 'candidatus mycoplasma wenyonii'. | cell-wall-less uncultivated parasitic bacteria that attach to the surface of host erythrocytes currently are classified in the order rickettsiales, family anaplasmataceae, in the genera haemobartonella and eperythrozoon. recently 16s rrna gene sequences have been determined for four of these species: haemobartonella felis and haemobartonella muris and eperythrozoon suis and eperythrozoon wenyonii. phylogenetic analysis of these sequence data shows that these haemotrophic bacteria are closely rel ... | 2001 | 11411711 |
| use of a polymerase chain reaction assay to detect infection with eperythrozoon wenyoni in cattle. | to determine whether a polymerase chain reaction (pcr) assay could be used to detect eperythrozoon wenyoni in the blood of cattle. | 2001 | 11724184 |
| revision of haemotrophic mycoplasma species names. | the recently proposed transfer of four rickettsias from the genera haemobartonella and eperythrozoon to the genus mycoplasma with the candidatus status is herein revised. this is because the candidatus designation is for new, incompletely described taxa, in order to give them a provisional status. thus, 'candidatus mycoplasma haemofelis' is revised to mycoplasma haemofelis comb. nov., nom. nov., 'candidatus mycoplasma haemomuris' is revised to mycoplasma haemomuris comb. nov., nom. nov., 'candid ... | 2002 | 11931184 |
| multiple infection in bovines from the tropics: observation of blood parasites by scanning and transmission electron microscope. | blood samples from a splenectomized bovine, experimentally inoculated with blood from a field cow living in southwestern venezuela, were processed for transmission and scanning electron microscopy. the blood sample showed multiple infection with hemoparasites of the genera anaplasma marginale, eperythrozoon wenyonii and trypanosoma vivax. scanning electron microscope showed that the blood from bovines with multiple infection had profound deformation in knob-like protruding structures with reduce ... | 2002 | 12701380 |
| morphologic, antigenic, and pathogenic characteristics of eperythrozoon ovis and eperythrozoon wenyoni. | | 1963 | 14035671 |
| concurrent infections with vector-borne pathogens associated with fatal hemolytic anemia in a cattle herd in switzerland. | bovine anaplasmosis is a vector-borne disease that results in substantial economic losses in other parts of the world but so far not in northern europe. in august 2002, a fatal disease outbreak was reported in a large dairy herd in the swiss canton of grisons. diseased animals experienced fever, anorexia, agalactia, and depression. anemia, ectoparasite infestation, and, occasionally, hemoglobinuria were observed. to determine the roles of vector-borne pathogens and to characterize the disease, b ... | 2004 | 15297529 |
| the detection of mycoplasma (formerly eperythrozoon) wenyonii by 16s rdna pcr and denaturing gradient gel electrophoresis. | although the role of mycoplasma wenyonii in disease is still subject to some debate, infections have been reported to result in parasitaemia, anaemia, scrotal and hind limb oedema, tachycardia, pyrexia, infertility, swollen teats, prefemoral lymphadenopathy and decreased milk production. previously, diagnosis of m. wenyonii has been based on blood smears but is not specific for m. wenyonii and can be difficult to interpret. we have previously described the use of pcr and denaturing gradient gel ... | 2006 | 16846699 |
| identification of a haemomycoplasma species in anemic reindeer (rangifer tarandus). | during an 18-mo period (may 2002-november 2003), 10 animals in a herd of 19 reindeer (rangifer tarandus) at the national animal disease center (nadc) experienced episodes of anemia. affected animals had histories of weight loss, unthriftiness, occasionally edema of dependent parts and moderate anemia characterized by microcytosis or macrocytosis, hypochromasia, schistocytosis, keratocytosis, acanthocytosis, and dacryocytosis. numerous basophilic punctate to ring-shaped bodies, measuring less tha ... | 2006 | 16870847 |
| molecular detection of mycoplasma wenyonii and 'candidatus mycoplasma haemobos' in cattle in hokkaido, japan. | blood samples from 78 cattle were tested for hemoplasma infection using molecular methods. pcr and sequence analysis revealed that 17 cattle were infected with mycoplasma wenyonii, while 13 were infected with 'candidatus mycoplasma haemobos'. four animals were infected with both species. this is the first study to report hemoplasma species infection among cattle in japan. | 2008 | 18571343 |
| differential detection of hemotropic mycoplasma species in cattle by melting curve analysis of pcr products. | we developed a real-time pcr procedure followed by melting curve analysis using the green fluorescence dye sybr green i for rapid detection and differentiation of hemplasmas in cattle. analysis of the melting temperature (tm) of the pcr products allowed for differentiation of the 2 bovine hemoplasmas, mycoplasma wenyonii and a provisional species, ;candidatus mycoplasma haemobos' (a synonym of ;candidatus m. haemobovis'). the tm (mean +/- s.e.) of the pcr products from the bovine hemoplasmas wer ... | 2010 | 19893280 |
| comparison of the effect of two hemoplasma species on hematological parameters in cattle. | mycoplasma wenyonii and ;candidatus mycoplasma haemobos' are pathogens associated with bovine hemoplasmosis. hematological parameters of these two hemoplasma species were compared in a cattle herd that was known to be infected with these 2 pathogens. ;c. m. haemobos'-infected cattle exhibited lower red blood cell levels, hemoglobin concentrations and packed cell volumes than m. wenyonii-infected cattle and hemoplasma-negative controls. on the other hand, cattle infected with m. wenyonii did not ... | 2010 | 19915333 |
| detection of candidatus mycoplasma haemobos in cattle with anaemia. | haemotrophic mycoplasmas are unculturable eperythrocytic pathogens that are found in a wide range of domestic and wild animals. in this study an outbreak of haemotrophic mycoplasmosis in cattle herds in northern germany is reported. affected animals exhibited anaemia and depression and infection was confirmed following microscopic examination of blood smears and on pcr. sequence analysis indicated that in addition to infection with mycoplasma wenyonii, animals were infected with a novel bovine h ... | 2011 | 20188610 |
| treatment of mycoplasma wenyonii infection in cows with imidocarb dipropionate injection-acupuncture. | sixteen shorthorn cows from xiazhuang farm were admitted to the veterinary teaching hospital at the college of animal science and veterinary medicine, shandong agricultural university for evaluation of poor appetite, listlessness, fever, tachycardia, tachypnea, lethargy, positive jugular venous pulse and anemia. blood smear examination and polymerase chain reaction analysis in these cows revealed an infection with mycoplasma wenyonii. the subjects were divided into two groups: control group (thr ... | 2008 | 20633467 |
| identification, molecular characterization, and occurrence of two bovine hemoplasma species in swiss cattle and development of real-time taqman quantitative pcr assays for diagnosis of bovine hemoplasma infections. | concomitantly with an outbreak of fatal anaplasmosis in a cattle herd in switzerland in 2002, we detected two bovine hemoplasma species in diseased animals: mycoplasma wenyonii (formerly eperythrozoon wenyonii) and a second, novel bovine hemoplasma species later designated "candidatus mycoplasma haemobos" (synonym, "candidatus mycoplasma haemobovis"). the second species was characterized by a shorter 16s rrna gene. the aims of the present study were to provide a detailed molecular characterizati ... | 2010 | 20686093 |
| fatal bovine anaplasmosis in a herd with new genotypes of anaplasma marginale, anaplasma ovis and concurrent haemoplasmosis. | haematological and molecular analysis of blood samples was carried out during an outbreak of bovine anaplasmosis in hungary. acute disease was observed in five animals, two of which died. anaplasma-carrier state was diagnosed in 69 (92%) of cattle. further evaluation of 24 blood samples revealed concurrent infections with mycoplasma wenyonii and 'candidatusm. haemobos' in 22 and 21 animals, respectively. in addition, two cows were identified with rickettsaemia. regarding molecular investigation ... | 2010 | 21094505 |
| molecular investigation of transplacental and vector-borne transmission of bovine haemoplasmas. | the present study was carried out in a herd with concurrent infections of mycoplasma wenyonii and 'candidatus m. haemobos', to investigate if transplacental and/or vector-borne transmission is possible for one or both bovine haemoplasma species. for this purpose blood samples were collected from 38 mother animals and their newborn calves; as well as from 17 uninseminated cows twice three months apart. in addition, 311 mosquitoes and blood-sucking flies (diptera: culicidae, tabanidae, muscidae) w ... | 2011 | 21605950 |
| prevalence of hemoplasma infection among cattle in the western part of japan. | we have examined for hemoplasma infection among cattle in the hiroshima and miyazaki prefectures by using a sensitive real-time pcr, with sybr green i and with melting curve analysis, which allow to distinguish the two bovine hemoplasma species, mycoplasma wenyonii and 'candidatus m. haemobos'. we found 69.4% of 36 cattle in hiroshima and 93.8% of 32 cattle in miyazaki infected with either of these two hemoplasma species. high morbidity in western part of japan may reflect the activity of arthro ... | 2011 | 21799295 |
| severe anemia associated with mycoplasma wenyonii infection in a mature cow. | the clinical findings, diagnostic tests, and treatment of clinical anemia in a mature angus cow infected with the hemoplasma mycoplasma wenyonii are described. mycoplasma wenyonii has been previously reported to cause clinical anemia in young or splenectomized cattle; however, infection has not been associated with severe anemia in mature animals. | 2011 | 22379205 |
| a case report of mycoplasma wenyonii associated immune-mediated haemolytic anaemia in a dairy cow. | a three year old, second lactation holstein dairy cow presented to the scottish centre for production animal health and food safety, glasgow university veterinary school in november 2014 with a history of post-calving vulval/vaginal bleeding nine days prior to presentation, followed by a sudden reduction in milk yield. subsequent investigations resulted in a diagnosis of immune-mediated haemolytic anaemia secondary to infection with mycoplasma wenyonii. | 2016 | 26807214 |
| detection of 'candidatus mycoplasma haemobos', mycoplasma wenyonii and anaplasma phagocytophilum from cattle in england. | | 2012 | 22517868 |
| prevalence and risk factor analysis of bovine hemoplasma infection by direct pcr in eastern hokkaido, japan. | the prevalence and risk factors of 2 bovine hemoplasma species-mycoplasma wenyonii (mw) and "candidatus mycoplasma haemobos" (cmh)-were examined by direct pcr using whole blood samples collected from 343 cattle in eastern hokkaido, japan. the sensitivity of the direct pcr assay was 10-fold higher than the standard pcr assay, detecting 5 copies of bovine hemoplasma dna per reaction. an epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was perfo ... | 2012 | 22673725 |
| assessment of the ability of aedes species mosquitoes to transmit feline mycoplasma haemofelis and 'candidatus mycoplasma haemominutum'. | the objective of this study was to evaluate wild-caught mosquitoes for evidence of hemotropic mycoplasma species dna and to determine whether the feline hemoplasmas, mycoplasma haemofelis (mhf) and 'candidatus mycoplasma haemominutum' (mhm), can be transmitted by aedes aegypti mosquitoes in a laboratory setting. | 2016 | 27418288 |
| mycoplasma wenyonii infection in dairy cows. | mycoplasma wenyonii infection in two dairy herdshypomagnesaemia in suckler cowsleptospiral milk drop in dairy cowsnon-resolving orf in six-month-old lambshepatosis dietetica in a five-month-old giltthese are among matters discussed in the disease surveillance report for september 2016 from sac consulting: veterinary services (sac c vs). | 2016 | 27980144 |
| vertical transmission of mycoplasma wenyonii in cattle, supported by analysis of the ribonuclease p rna gene - short communication. | the vertical transmission of mycoplasma (m.) wenyonii was investigated in beef cattle raised on a farm in japan by analysing the ribonuclease p rna (rnpb) gene sequence using pcr. peripheral blood samples from 17 dams infected with m. wenyonii and from their neonatal calves were collected and colostrum samples were taken from cows immediately after parturition, and subsequently the blood samples of calves were monitored continuously for three months. at birth on day 0, although no rnpb gene was ... | 2015 | 26551417 |
| investigation of bovine haemoplasmas and their association with anaemia in new zealand cattle. | a dairy cow, from a herd in the waikato region of new zealand, was reported with regenerative anaemia on 12 september 2014. testing of blood from the animal using pcr assays for theileria orientalis produced a negative result for both chitose and ikeda types. | 2016 | 26411673 |
| effect of chronic hemoplasma infection on cattle productivity. | the present study evaluated the effect of hemoplasmosis on cattle productivity. prevalence of bovine hemoplasma was examined by polymerase chain reaction (pcr) using whole blood samples collected from 93 breeding cows and their 71 calves in hokkaido, japan. monthly milk production records and other clinical data were compared between mycoplasma wenyonii (mw)-infected, "candidatus mycoplasma haemobos" (cmh)-infected, co-infected and pcr-negative groups. blood chemical parameters were obtained fro ... | 2013 | 23676278 |
| [haemoplasma infection in a dairy cow]. | the present work describes the clinical and laboratory examination as well as the treatment of a 7-year-old local dairy breed cow presented with reduced appetite, decreasing milk yield and striking yellowish discoloured skin and mucosa. the laboratory examination revealed a high degree regenerative anaemia and hyperbilirubinaemia. the bovine haemotrophic mycoplasma species mycoplasma wenyonii and 'candidatus mycoplasma haemobos' were detected in the blood by pcr. treatment with oxytetracycline r ... | 2012 | 23242150 |
| complete genome sequence of mycoplasma wenyonii strain massachusetts. | mycoplasma wenyonii is a hemotrophic mycoplasma that causes acute and chronic infections in cattle. here, we announce the first complete genome sequence of this organism. the genome is a single circular chromosome with 650,228 bp and g+c% of 33.9. analyses of m. wenyonii genome will provide insights into its biology. | 2012 | 22965086 |
| detection of mycoplasma wenyonii in cattle and transmission vectors by the loop-mediated isothermal amplification (lamp) assay. | mycoplasma wenyonii is a wall-less hemotrophic prokaryote with worldwide distribution. this paper describes the development of a lamp method targeting 16s rrna for specific detection of m. wenyonii in its vectors and cattle. the lamp method is specific for m. wenyonii detection and more sensitive than pcr. a total of 330 blood samples from cattle were tested by lamp and pcr detection, 71 (21.5 %) samples were positive by the lamp, while only 62 (18.8 %) were positive by pcr. for detecting transm ... | 2013 | 22684637 |