comparison of restriction fragment length polymorphisms of ribosomal dna between diphyllobothrium nihonkaiense and d. latum. | restriction fragment length polymorphisms (rflps) of ribosomal dna (rdna) were compared between diphyllobothrium latum and d. nihonkaiense using seven kinds of restriction endonucleases. no intra-specific variation in restriction fragment profiles was shown within both species of diphyllobothrium. digestion of the genomic dna with three endonucleases. smai, hinfi and hhai, provided one or two different bands between two species, although the hybridization patterns generated with the others. hind ... | 1992 | 1363430 |
[a human infection of the cestode, diphyllobothrium nihonkaiense yamane et al., 1986]. | a mature cestode, obtained from a 49-year-old man in fukuoka, southern japan, was examined. the worm was about 70 cm in length without scolex. the largest segment was 1.8 x 0.8 cm. cirrus sac lay at an obtuse angle (about 150 degrees) to seminal vesicle. the eggs were 62.06 +/- 9.40 x 43.33 +/- 6.43 microns and the shell thickness 1.0-1.5 microns, with round, shallow and sparsely distributed surface pits ranging 0.8-2.0 microns in diameter. the above features suggested that this worm was identif ... | 1989 | 2807129 |
praziquantel for the treatment of diphyllobothrium nihonkaiense infections in humans. | | 1994 | 7992346 |
single dose treatment with praziquantel for human diphyllobothrium nihonkaiense infections. | diphyllobothrium nihonkaiense is a large pseudophyllid cestode and one of the commonest parasites derived from fish in japan. three japanese patients infected with d. nihonkaiense were treated with a single oral dose of 7, 23 or 25 mg/kg of praziquantel. all patients expelled the tapeworm with scolex, and no side effect was noted. this study indicates that single-dose treatment with praziquantel is effective against d. nihonkaiense infection. | 1993 | 8249091 |
five cases of diphyllobothrium nihonkaiense infection with discovery of plerocercoids from an infective source, oncorhynchus masou ishikawae. | five persons from 2 families residing at miyama town, mie prefecture, japan, ingested fresh raw fish oncorhynchus sp. on 9 may 1999 that was caught at owase district in mie. they all expelled diphyllobothriid cestodes 11-37 days after ingesting the fish. the parasites were morphologically identical to diphyllobothrium nihonkaiense yamane et al., 1986. five plerocercoids were detected from a portion of the fish. nucleotide sequence of a region of the cytochrome c oxidase subunit i gene of mitocho ... | 2001 | 11227909 |
a case of diphyllobothrium nihonkaiense infection successfully treated by oral administration of gastrografin. | a diphyllobothriid cestode infection found in a 54-year-old male residing in oita, japan, was successfully treated by oral administration of gastrografin in combination with a intramuscular injection of vagostigmin. the strobila expelled was 6.14 m long with a scolex, and morphologically identical with diphyllobothrium nihonkaiense except unusual ovaries of which posterior horns were confluent in each proglottid. this is the first case of treatment of cestode infection by oral administration of ... | 1999 | 11269276 |
single low-dose treatment with praziquantel for diphyllobothrium nihonkaiense infections. | to investigate whether praziquantel is effective against diphyllobothriasis nihonkaiense at doses less than the conventional dose. | 2003 | 12583616 |
putative diphyllobothrium nihonkaiense acquired from a pacific salmon (oncorhynchus keta) eaten in france; genomic identification and case report. | we report here a likely case of diphyllobothrium nihonkaiense contracted in france through the consumption of a pacific salmon imported from canada. the species diagnosis was made by molecular analysis of two mitochondrial genes (coi & nd3). this case is rather unusual in that d. nihonkaiense has never been reported along the pacific coast of north america. | 2006 | 16243582 |
mitochondrial genomes of the human broad tapeworms diphyllobothrium latum and diphyllobothrium nihonkaiense (cestoda: diphyllobothriidae). | mitochondrial dna (mtdna) sequences of the human broad tapeworms diphyllobothrium latum and diphyllobothrium nihonkaiense have been totally determined. both of them are closed circular molecules (total length, 13,720 bp in d. latum and 13,747 bp in d. nihonkaiense) containing genes for 12 proteins, 22 transfer rnas, and two ribosomal rnas. all the genes are coded on t-rich strand. the gene order of diphyllobothrium mtdnas is completely identical with that of taenia and echinococcus mtdnas. the o ... | 2007 | 17252274 |
diphyllobothrium nihonkaiense (yamane et al., 1986) in switzerland: first molecular evidence and case reports. | we report the first cases of locally-acquired diphyllobothrium nihonkaiense (yamane, kamo, bylund and wikgren, 1986) in switzerland, confirmed by genetic analysis (18s rrna, coi and its1-5.8s rrna-its2 genes). diphyllobothriasis in this country is attributed to the tapeworm d. latum (linnaeus, 1758) but the increasing popularity of raw fish culinary specialities (sushi, carpaccio, tartare) brings out a new diagnostic problem, so that people can get infected by exotic species of tapeworms. | 2007 | 17369079 |
characterization of the complete mitochondrial genome of diphyllobothrium nihonkaiense (diphyllobothriidae: cestoda), and development of molecular markers for differentiating fish tapeworms. | we sequenced and characterized the complete mitochondrial genome of the japanese fish tapeworm d. nihonkaiense. the genome is a circular-dna molecule of 13607 bp (one nucleotide shorter than that of d. latum mtdna) containing 12 protein-coding genes (lacking atp8), 22 trna genes and two rrna genes. gene order and genome content are identical to those of the other cestodes reported thus far, including its congener d. latum. the only exception is hymenolepis diminuta in which the positions of trns ... | 2007 | 17646713 |
diphyllobothriasis nihonkaiense: possibly acquired in switzerland from imported pacific salmon. | a 5-year-old japanese boy passed tapeworm strobila while he was living in switzerland. during a short visit to japan, he was successfully treated with a single dose of praziquantel. dna sequences of its1, cox1 and nd3 genes from the tapeworm were compatible with those of diphyllobothrium nihonkaiense rather than diphyllobothrium latum, which is prevalent in europe. the patient consumed imported salmon in switzerland. this case highlights the globalization of d. nihonkaiense, which was once restr ... | 2008 | 18628587 |
a 2-year-old girl with diphyllobothrium nihonkaiense infection treated with oral praziquantel and a laxative. | we report a case of diphyllobothrium nihonkaiense infection in a 2-year-old japanese girl. when infection occurs in early childhood, it is necessary to offer supportive care in addition to standard treatment with oral praziquantel or with of a duodenal tube using a radiopaque contrast medium. we treated d. nihonkaiense infection in a 2-year-old girl successfully treated with oral praziquantel and a laxative. | 2008 | 18781046 |
[diphyllobothrium nihonkaiense infection: a new risk in relation with the consumption of salmon]. | | 2009 | 18834694 |
mitochondrial dna divergence in populations of the tapeworm diphyllobothrium nihonkaiense and its phylogenetic relationship with diphyllobothrium klebanovskii. | diphyllobothrium nihonkaiense [y. yamane, h. kamo, g. bylund, j.p. wilkgren. diphyllobothrium nihonkaiense sp. nov (cestoda: diphyllobothriidae)- revised identification of japanese broad tapeworm. shimane j med sci 1986;10:29-48.] and diphyllobothrium klebanovskii [i.v. muratov, p.s. posokhov. causative agent of human diphyllobothriasis - diphyllobothrium klebanovskii sp. n. parazitologiia. 1988;22:165-170.] are two major species of human diphyllobothriasis in japan and far east russia, respecti ... | 2009 | 18835460 |
a case of diphyllobothrium nihonkaiense infection possibly linked to salmon consumption in new zealand. | global increases in salmon consumption and changes in fish-eating habits have meant that diphyllobothrium infections now occur in regions where they were previously absent. in the present paper, a case of diphyllobothriasis through the likely ingestion of raw salmon in new zealand in a japanese patient is reported. the causative tapeworm species was identified as diphyllobothrium nihonkaiense based on mitochondrial dna analysis of proglottid expelled from the patient. | 2009 | 19444469 |
[prevalence of intestinal canine and feline parasites in saitama prefecture, japan]. | we studied the prevalence of intestinal parasites in animal companions in saitama prefecture, japan, where no detailed data is currently available. between may 1999 and december 2007, fecal samples were collected from 906 dogs and 1,079 cats in public animal shelters and examined by microscopy. overall, prevalence of intestinal parasites in dogs was 38.6% and cats 43.1%. trichuris vulpis was the most prevalent canine parasite species (22.3%), followed by toxocara canis (12.5%), ancylostoma canin ... | 2009 | 19522305 |
diphyllobothriasis associated with eating raw pacific salmon. | the incidence of human infection with the broad tapeworm diphyllobothrium nihonkaiense has been increasing in urban areas of japan and in european countries. d. nihonkaiense is morphologically similar to but genetically distinct from d. latum and exploits anadromous wild pacific salmon as its second intermediate host. clinical signs in humans include diarrhea and discharge of the strobila, which can be as long as 12 m. the natural life history and the geographic range of the tapeworm remain to b ... | 2009 | 19523283 |
inter- and intra-specific characterization of tapeworms of the genus diphyllobothrium (cestoda: diphyllobothriidea) from switzerland, using nuclear and mitochondrial dna targets. | human diphyllobothriosis is caused by at least 14 species of cestodes belonging to the genus diphyllobothrium. molecular analysis by sequencing of nuclear and mitochondrial targets identifies some species at inter- and intra-specific level, and helps to reconstruct their phylogenetic relationships. nevertheless, the suitability of further molecular targets deserves to be widened, and the comparison of samples of different geographical origin could allow their intra-specific characterization, whi ... | 2010 | 19800982 |
morphologic and genetic identification of diphyllobothrium nihonkaiense in korea. | diphyllobothrium nihonkaiense was first described by yamane in 1986 but the taxonomical features have been obscure due to lack of critical morphologic criteria in its larval and adult stages. in korea, this tapeworm had long been known as diphyllobothrium latum. in this study, we observed 62 specimens collected from korean residents and analyzed them by morphological features and nucleotide sequences of mitochondrial cox1 gene as well as the its1 region. adult tapeworms were examined after carmi ... | 2009 | 19967084 |
anomalous segmentation of diphyllobothrium nihonkaiense. | an anomalous tapeworm with abnormal segmentation was obtained from a 6-year-old boy in japan. the tapeworm consisted of proglottids with slanted anterior and posterior margins of proglottids and 4-6 sets of reproductive organs arranged between the margins. the morphology of the tapeworm did not correspond to any of the described cestodes. however, molecular identification based on nuclear and mitochondrial genes clearly showed the tapeworm was diphyllobothrium nihonkaiense. | 2010 | 20035897 |
detection and identification of diphyllobothrium nihonkaiense plerocercoids from wild pacific salmon (oncorhynchus spp.) in japan. | we investigated the risk of diphyllobothriasis from ingestion of wild pacific salmon in japan by surveying diphyllobothrium plerocercoids in 182 salmon samples obtained from japan. the plerocercoids were not detected in chum salmon (oncorhynchus keta) (0/26), called akizake in japan, caught between september and november. however, the detection rate of plerocercoids in chum salmon, called tokishirazu in japan, caught between early april and june, was 51.1% (24/47) with an average of two plerocer ... | 2010 | 20334715 |
[a case of diphyllobothrium nihonkaiense identified by virtual enterography and virtual enteroscopy]. | in october 2007, a woman in her early 30s presented, complaining of worm discharge in her stool. we identified it as diphyllobothrium nihonkaiense, and identified it by virtual enterography and virtual enteroscopy. it was treated by 1500 mg/day praziquantel orally. we think these methods, of pathology as well as capsule enteroscopy and enteroscopy, are useful for the searching of the small intestinal lesions. we hope these examinations will become more widespread with reference to these cases. | 2010 | 20693755 |
[molecular identification of human diphyllobothrium nihonkaiense using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequence]. | identification of diphyllobothrium species has been carried out based on their morphology, especially sexual organs. in addition to these criteria, pcr-based identification methods have been developed recently. a 20 year-old japanese living in kochi prefecture passed tapeworm. he was successfully treated with single dose of gastrografin. we examined the morphologic features of the proglottids and eggs using histology and scanning electron microscope. we also analyzed mitochondrial cytochrome c o ... | 2010 | 21229706 |
long fish tapeworm in the intestine: an in situ observation by capsule endoscopy. | we present the case of a patient who was referred to our hospital after she reported having passed a long, whitish object per rectum. accordingly, capsule endoscopy was performed using the pillcam(®) sb video capsule. a tapeworm of the species diphyllobothrium nihonkaiense was detected; it appeared to be freely floating and unfolded in the jejunum and sometimes tangled or irregularly folded in the ileum. the stretching of the strobila by strong peristalsis in the ileum may have resulted in the s ... | 2011 | 21325765 |
two pediatric cases of diphyllobothrium nihonkaiense infection in summer 2010. | we report two pediatric cases (6- and 8-year-old japanese boys) of diphyllobothrium nihonkaiense infection occurring in july and august 2010. both patients were asymptomatic, but presented to our hospital after passing tapeworm segments in their stool. a single dose of praziquantel was administered to each patient, and both patients expelled segments. the tapeworms were identified as d. nihonkaiense by mitochondrial cytochrome c oxidase subunit 1 gene analysis. the patients have shown no recurre ... | 2011 | 22142556 |
[case report; a case of diphyllobothrium nihonkaiense infection probably caused by eating raw trout]. | | 2011 | 22250428 |
rapid identification of nine species of diphyllobothriidean tapeworms by pyrosequencing. | the identification of diphyllobothriidean tapeworms (cestoda: diphyllobothriidea) that infect humans and intermediate/paratenic hosts is extremely difficult due to their morphological similarities, particularly in the case of diphyllobothrium and spirometra species. a pyrosequencing method for the molecular identification of pathogenic agents has recently been developed, but as of yet there have been no reports of pyrosequencing approaches that are able to discriminate among diphyllobothriidean ... | 2016 | 27853295 |
first confirmed human case of diphyllobothrium stemmacephalum infection and molecular verification of the synonymy of diphyllobothrium yonagoense with d. stemmacephalum (cestoda: diphyllobothriidea). | several tapeworm species in the genus diphyllobothrium cobbold, 1858 have uncertain taxonomic positions, leading to taxonomic confusion as well as misdiagnosis of infections. taxonomic revision based on dna sequence analysis is considered necessary to resolve the taxonomy of several cases, including that between diphyllobothrium stemmacephalum, the type species of the genus, and diphyllobothrium yonagoense. diphyllobothrium yonagoense was synonymized with d. stemmacephalum based on morphological ... | 2016 | 27266483 |
diphyllobothrium nihonkaiense: wide egg size variation in 32 molecularly confirmed adult specimens from korea. | the eggs of diphyllobothrium nihonkaiense were reported to be smaller than those of the classical diphyllobothrium latum in general. however, verification using a large number of adult tapeworms is required. we assessed the egg size variation in 32 adult specimens of d. nihonkaiense recovered from korean patients in 1975-2014. the diagnosis of individual specimens was based on analysis of the mitochondrial cytochrome c oxidase 1 gene sequence. uterine eggs (nā=ā10) were obtained from each specim ... | 2015 | 25758587 |
diphyllobothrium nihonkaiense infections in a family. | diphyllobothrium latum and diphyllobothrium nihonkaiense are morphologically similar to each other, and only genetic method can differentiate clearly between the 2 species. a strobila of diphyllobothriid tapeworm discharged from a 7-year-old boy was analyzed to identify the species by mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequencing. he and his family (total 4 persons) ate slices of 3 kinds of raw fish 16 days before visiting our outpatient clinic. all family members complaine ... | 2015 | 25748717 |
four additional cases of diphyllobothrium nihonkaiense infection confirmed by analysis of cox1 gene in korea. | most of the diphyllobothriid tapeworms isolated from human samples in the republic of korea (= korea) have been identified as diphyllobothrium nihonkaiense by genetic analysis. this paper reports confirmation of d. nihonkaiense infections in 4 additional human samples obtained between 1995 and 2014, which were analyzed at the department of parasitology, hallym university college of medicine, korea. analysis of the mitochondrial cytochrome c oxidase 1 (cox1) gene revealed a 98.5-99.5% similarity ... | 2015 | 25748716 |
human diphyllobothrium nihonkaiense infection in washington state. | a patient in washington state harbored a fish tapeworm most likely acquired from eating raw salmon. diphyllobothrium nihonkaiense was identified by cox1 sequence analysis. although this is the first documented human d. nihonkaiense infection in the united states, the parasite may have been present earlier but misidentified as diphyllobothrium latum. | 2015 | 25609724 |
three cases of diphyllobothrium nihonkaiense infection in korea. | until 2012, a total of 48 cases of diphyllobothriasis had been reported in korea, all of which were morphologically identified as diphyllobothrium latum. however, some of these specimens were analyzed by nucleotide sequencing of the mitochondrial cox1 gene, which showed that all were d. nihonkaiense, not d. latum. after that, 3 further cases of diphyllobothriasis were confirmed as d. nihonkaiense. in the present study, 3 new cases of d. nihonkaiense were detected from 2011 through 2013. the host ... | 2014 | 25548420 |
two human cases of diphyllobothrium nihonkaiense infection in korea. | diphyllobothrium latum and diphyllobothrium nihonkaiense are the 2 reported main causes of human diphyllobothriasis in the republic of korea. however, the differentiation of these 2 species based on morphologic features alone is difficult. the authors used nucleotide sequencing of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene to diagnose diphyllobothrium spp. two patients visited the emergency room at kyungpook national university hospital on 3 april and 12 april 2013, respectivel ... | 2014 | 24850965 |
molecular detection of diphyllobothrium nihonkaiense in humans, china. | the cause of diphyllobothriosis in 5 persons in harbin and shanghai, china, during 2008-2011, initially attributed to the tapeworm diphyllobothrium latum, was confirmed as d. nihonkaiense by using molecular analysis of expelled proglottids. the use of morphologic characteristics alone to identify this organism was inadequate and led to misidentification of the species. | 2014 | 24447495 |
a case of diphyllobothrium nihonkaiense infection as confirmed by mitochondrial cox1 gene sequence analysis. | diphyllobothrium nihonkaiense has been reported in korea as diphyllobothrium latum because of their close morphologic resemblance. we have identified a human case of d. nihonkaiense infection using the mitochondrial cytochrome c oxidase subunit i (cox1) gene sequence analysis. on 18 february 2012, a patient who had consumed raw fish a month earlier visited our outpatient clinic with a long tapeworm parasite excreted in the feces. the body of the segmented worm was 2 m long and divided into the s ... | 2013 | 24039292 |
diphyllobothrium nihonkaiense tapeworm larvae in salmon from north america. | diphyllobothriosis is reemerging because of global importation and increased popularity of eating raw fish. we detected diphyllobothrium nihonkaiense plerocercoids in the musculature of wild pink salmon (oncorhynchus gorbuscha) from alaska, usa. therefore, salmon from the american and asian pacific coasts and elsewhere pose potential dangers for persons who eat these fish raw. | 2017 | 28098540 |
molecular identification of diphyllobothrium nihonkaiense from 3 human cases in heilongjiang province with a brief literature review in china. | human diphyllobothriasis is a widespread fish-borne zoonosis caused by the infection with broad tapeworms belonging to the genus diphyllobothrium. in mainland china, so far 20 human cases of diphyllobothrium infections have been reported, and the etiologic species were identified as d. latum and d. nihonkaiense based on morphological characteristics or molecular analysis. in the present study, proglottids of diphyllobothriid tapeworms from 3 human cases that occurred in heilongjiang province, ch ... | 2015 | 26797434 |
extracorporeal worm extraction of diphyllobothrium nihonkaiense with amidotrizoic acid in a child. | infection cases of diphyllobothriid tapeworms are not much in the below teen-age group. we report a case of diphyllobothrium nihonkaiense infection in a 13-year-old boy. he presented with severe fatigue, occasional abdominal pain at night time. he also had several episodes of tapeworm segment discharge in his stools. by his past history, he had frequently eaten raw fish including salmon and trout with his families. numerous eggs of diphyllobothriid tapeworm were detected in the fecal examination ... | 2014 | 25548421 |
diphyllobothrium nihonkaiense infection linked to chilled salmon consumption. | this is a case of a 40-year-old japanese adult male who had acute onset watery diarrhoea with intermittent abdominal discomfort. several days later, he found a 1 metre of tape-shaped object emerged from his anus and was eventually admitted to our hospital for further investigation. stool examination revealed eggs of diphyllobothrium with characteristic shape in his stool. after oral praziquantel administration, segments of proglottids were expelled from his anus. the proglottids were sent to the ... | 2012 | 22665864 |
capsule endoscopy is a feasible procedure for identifying a diphyllobothrium nihonkaiense infection and determining the indications for vermifuge treatment. | diphyllobothrium is a member of cestoda family, which is the largest parasite of humans. the diagnosis of diphyllobothriasis is based on the detection of eggs in the stool. because the remainder of the scolex causes a relapse in diphyllobothriasis, the scolex must be completely discharged to cure the parasite infection. however, the scolex or forefront of the diphyllobothrium is difficult to detect with gastroduodenoscopy and colonoscopy, because most diphyllobothrium attach to the jejunal wall. ... | 2010 | 22767523 |
complete sequence and characterization of the mitochondrial genome of diphyllobothrium stemmacephalum, the type species of genus diphyllobothrium (cestoda: diphyllobothriidae), using next generation sequencing. | we first constructed and characterized the complete mitochondrial genome (mitogenome) sequence of diphyllobothrium stemmacephalum, the type species of genus diphyllobothrium, using next generation sequencing (ngs). the mitogenome of d. stemmacephalum was 13,716bp, including 12 protein-coding genes, 22 trna genes, 2 rrna genes and 2 longer intergenic non-coding regions, and has features common to mitogenomes of other cestodes. although it has been accepted that trna for serine (trns2(ucn)) in pla ... | 2017 | 28647499 |
four human cases of diphyllobothrium nihonkaiense (eucestoda: diphyllobothriidae) in china with a brief review of chinese cases. | we described 4 human infection cases of zoonotic fish-tapeworm, diphyllobothrium nihonkaiense, identified with morphological and molecular characters and briefly reviewed chinese cases in consideration of it as an emerging parasitic disease in china. the scolex and mature and gravid proglottids of some cases were seen, a rosette-shaped uterus was observed in the middle of the mature and gravid proglottids, and the diphyllobothriid eggs were yellowish-brown in color and displayed a small knob or ... | 2017 | 28719957 |
validity of the bear tapeworm diphyllobothrium ursi (cestoda: diphyllobothriidae) based on morphological and molecular markers. | the bear tapeworm diphyllobothrium ursi is described based upon the morphology of adult tapeworms recovered from the brown bear (ursus arctos middendorffi) and larval plerocercoids found in sockeye salmon (oncorhynchus nerka) from kodiak island in alaska in 1952. however, in 1987 d. ursi was synonymized with diphyllobothrium dendriticum, and the taxonomic relationship between both species has not subsequently been revised. in this study mitochondrial cytochrome c oxidase subunit 1 gene (cox1) se ... | 2012 | 22663179 |
first record of human infection with the tapeworm diphyllobothrium nihonkaiense in north america. | the tapeworm diphyllobothrium nihonkaiense (cestoda: diphyllobothriidea), originally described from japan, is reported from a man in north america for the first time. species identification was based on sequences of ribosomal (partial 18s rrna) and mitochondrial (partial cytochrome c oxidase subunit i) genes of proglottids expelled from a czech tourist who ate raw pacific sockeye salmon (oncorhynchus nerka) from british columbia, canada. | 2008 | 18256421 |