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determination of mycelial steroids with the aid of polyene antibiotics. ii. quantitative determination of the mycelial steroids of mucor hiemalis.uv absorption spectrum of polyene antibiotics was dramatically altered in the presence of steroids. the introduction of a steroid to an aqueous solution of polyene antibiotics resulted in a change in the ratio of absorbance of peak 3 (with shorter wavelength) to peak 1 (with longer wavelength), e3/e1. interactions between 28 steroids and 4 polyene antibiotics were studied and the results revealed that the structures of steroids essential for the optimal interaction with polyene antibiotics were ...19761036984
keratinophilic fungi and other moulds associated with air-dust particles from egypt.one-hundred and eleven species and three species varieties belonging to 39 genera were collected from 50 dust samples on the five media used at 28 degrees c. using the hair-baiting technique with horse hair, 10 species of chrysosporium were isolated: c. asperatum, c. state of arthroderma tuberculatum, c. indicum, c. inops, c. keratinophilum, c. merdarium, c. pannorum, c. queenslandicum, c. tropicum and c. xerophilum. true dermatophytes were isolated: trichophyton verrucosum and trichophyton sp. ...19901702081
[the formation of all-trans-phytoene in mucor hiemalis]. 19725070654
[the effect of aflatoxin b1 on the cytochemically detectable activity of various enzymes of mucor hiemalis (mucorales)]. 19704322088
[mycoflora of hay and straw].studies were carried out on hay and straw to establish the composition of mycoflora in them. they were sampled both at the time of harvesting and after storing for four to five months. it was found that the hay and straw were most often inseminated with spores of the fungus species alternaria tenuis, aspergillus fumigatus, mucor hiemalis, penicillium sp., and more rarely with spores of fusarium, chaetomium, scopulariopsis, cladosporium, trichoderma, and stachybotrys alternans. it was demonstrate ...19863739224
[metabolism of some phenolic derivatives in mucor hiemalis]. 19724660175
distribution of adenosine 5'-triphosphate (atp)-dependent hexose kinases in microorganisms.a systematic study of adenosine triphosphate (atp)-dependent hexose kinases among microorganisms has been undertaken. sixteen hexose kinases of five major types were partially purified from 12 microorganisms and characterized with respect to specificity for sugar and nucleotide substrates and michaelis constants for the sugar substrates. glucokinase activities that phosphorylate glucose and glucosamine are inhibited by n-acetyl-glucosamine and xylose, were found to be present in the non-sulphur ...1978214181
fungi in two swimming pools in assiut town, egypt.50 water samples from 2 public swimming pools were tested for the presence of fungi using the hair baiting technique and plate count methods. sabouraud's-dextrose agar and littman-oxgall agar media were used for isolation of the fungi. of the keratinolytic fungi 8 species were recorded of which three species were dermatophytes (trichophyton terrestre, 14% of the samples; t. mentagrophytes, 10% and microsporum gypseum, 6%). on littman-oxgall agar the following species were recovered: aspergillus ...19892475993
subcutaneous mucormycosis caused by mucor hiemalis wehmer f. luteus (linnemann) schipper 1973.the authors report a case of subcutaneous mucormycosis in a diabetic patient with verrucous lesions in the fourth finger of the right hand. initially diagnosed as a fixed cutaneous sporotrichosis case, success was obtained with the use of potassium iodide with healing of the lesion in a period of nearly 5 months. at present the patient remains under clinical observation with no treatment or relapse.19902267000
primary cutaneous mucormycosis in a healthy young girl. report of a case caused by mucor hiemalis wehmer.we report an unusual case of primary cutaneous mucormycosis caused by mucor hiemalis wehmer that occurred in a healthy young girl after an insect bite. the patient had a slowly extending, indurated, erythematous, and scaling eruption on the right cheek. histologic examination revealed granulomatous dermatitis and characteristic broad, nonseptate, pale-staining hyphae. this is the first report of an infection in a human being caused by mucor hiemalis wehmer. the infection was cured with intraveno ...19912050857
antibiotic activity of the pyrenocines.pyrenocine a, a phytotoxin produced by pyrenochaeta terrestris (hansen) gorenz, walker and larson, possesses general antibiotic activity against plants, fungi, and bacteria. effective doses for 50% inhibition (ed50s) are 4 micrograms/ml for onion seedling elongation; 14, 20, 20, and 25 micrograms/ml for the germination of asexual spores of fusarium oxysporum f. sp. cepae, fusarium solani f. sp. pisi, mucor hiemalis, and rhizopus stolonifer, respectively. pyrenocine a also inhibits the linear myc ...19873594310
metabolic products of microorganisms. 243. pyridazomycin, a new antifungal antibiotic produced by streptomyces violaceoniger.pyridazomycin, a new antifungal antibiotic produced by streptomyces violaceoniger sp. griseofuscus (strain tü 2557), was detected in a selective screening against mucor hiemalis (tü 179/180). the amino acid side chain of 1 can be seen as l-ornithine, whose gamma-nitrogen atom is part of a pyridazine ring building a quaternary ammonium system. the structure of 1 was established by spectroscopic analysis of the parent compound and degradation products. the occurrence of a pyridazine ring in microb ...19883384747
purification and characterization of a novel fungal endo-beta-n-acetylglucosaminidase acting on complex oligosaccharides of glycoproteins.a novel endo-beta-n-acetylglucosaminidase acting on complex type sugar chains of glycoproteins was found in the culture broth of a fungus isolated from soil and identified as mucor hiemalis f. hiemalis on the basis of various characteristics. the endo-beta-n-acetylglucosaminidase, named endo-m, was purified to almost homogeneity by polyacrylamide gel electrophoresis involving ammonium sulfate fractionation, and column chromatographies on deae-sepharose cl-6b, sephadex g-200, hydroxylapatite, tsk ...19901368528
an endo-(1 lead to 6) -beta-d-glucanase from mucor hiemalis.an endo-(1 lead to 6)-beta-d-glucanase (ec 3.2.1), isolated from the culture filtrate of mucor hiemalis, was purified by ammonium sulphate fractionation and gel filtration. the homogeneity of the enzyme was confirmed by disc electrophoresis. the enzyme had a wide range of temperature and ph stability, high substrate specificity, and an action pattern of the endo-type.1976947537
[phospholipase c of fungi and staphylococci].the activity of phospholipase was studied in the cultural broth and cell extract of 112 strains of fungi and yeasts. the endoenzyme was detected in 19 strains of mycelial fungi, the exoenzyme was found in mucor hiemalis 50 and aspergillus niger 117. phospholipase c of m. hiemalis was purified and compared to phospholipase of staphylococci. the values of km are 8.9 and 1.07 mm, respectively, for the fungal and staphylococcal enzymes.1976187904
an endo-(1 leads to 3)-beta-d-glucanase from mucor hiemalis.an endo-(1 leads to 3)-beta-d-glucanase (ec 3.2.1.6), isolated from the culture filtrate of the fungus mucor hiemalis, was purified by ammonium sulfate fractionation, gel filtration, and column chromatography on o-(carboxymethyl)cellulose. the optimum ph, optimum temperature, and km value of the enzyme were ph 5.0, 50 degrees, and 0.048%, respectively. the enzyme was strongly inactivated by pb2+, cu2+, and hg2+ ions and also inhibited by zn2+ and fe3+ ions. the enzyme was specific for laminaran ...197716694
effect of triglycerides on the production of lipids and lipase by mucor hiemalis.the effect of triglycerides on the growth of mucor hiemalis and the production of lipase and mycelial lipids was studied. addition of 1% triglycerides to the fermentation medium was best for the mycelial as well as the broth lipase production. the added triglycerides seemed to be utilized through the formation of free fatty acids, and towards the end of the growth phase most of the triglycerides and their hydrolysis products were utilized. the mycelial lipase activity was maximum (66 u/g dry myc ...19836883224
[influence of anaerobiosis on the sterol composition of mucor hiemalis].strict anaerobic cultures of mucor hiemalis lead to major modifications of the quantitative and qualitative composition of sterols. sterols of which the lateral chain contains an ethyl or ethylidene group in c24 are for the first time brought to evidence in a mucorales. isolation in the same conditions of e-ethylidene-24 (28) lanostene-8 ol-3 beta lets us suppose that sterols, which are absent in aerobic cultures, are derived from this triterpene of 32 c atoms.19836883220
cytological effects of fungal metabolites produced by fungi isolated from egyptian poultry feedstuffs.the cytogenetic effects of fungal metabolites produced by 113 strains belonging to 36 fungal species and isolated form 5 substrates of commercial poultry feedstuffs were tested for their effect on the growing root meristems of allium cepa. the fungal metabolites of paecilomyces canescens, aspergillus fumigatus, syncephalastrum racemosum, aspergillus terreus and mucor hiemalis strongly suppressed cell division. metabolites from other strains had less effect on cell division but permitted the appe ...19892746472
keratinolytic and keratinophilic fungi in the soils of papua new guinea.a study was made of soil samples collected during an expedition to the highlands of papua new guinea. fungi were isolated from the samples by the method of hair baiting (to-ka-va). of the 33 species isolated, about half showed keratinolytic activity. such activity is previously unreported for mucor hiemalis f. hiemalis, myrothecium roridum, paecilomyces carneus, p. marquandii, penicillium brevicompactum, rhinocladiella mansonii and verticillium lecanii. the species most active keratinolytically ...19911723786
release of proteinase from mycelium of mucor hiemalis.when mucor hiemalis nrrl 3103 was grown in soybean medium, only a small fraction of the proteinase produced by the organism appeared in the culture filtrate, whereas the bulk of the enzyme was bound to the mycelial surface. optimal ph of the proteinase ranged from 3.0 to 3.5. inclusion of sodium chloride or other ionizable salts in the growth medium, however, resulted in the liberation from the mycelium of the loosely bound enzyme as it was formed. maximal release of proteinase was achieved at a ...19676025300
microbial endo-beta-n-acetylglucosaminidases acting on complex-type sugar chains of glycoproteins.the activity and substrate specificity of endo-beta-n-acetylglucosaminidase [glycopeptide-d-mannosyl-n4-(n-acetyl-d-glucosaminyl)2-asparagine 1,4-n-acetyl-beta-glucosamino-hydrolase, ec 3.2.1.96] obtained from mucor hiemalis (endo-m) was compared with that of the enzyme obtained from flavobacterium meningosepticum (endo-f), which is the only enzyme available that acts on the complex oligosaccharides of asparagine-linked sugar chains in glycoproteins. they showed almost the same activities toward ...19911718952
myxothiazol, an antibiotic from myxococcus fulvus (myxobacterales). i. cultivation, isolation, physico-chemical and biological properties.myxothiazol (ab-mx f16-1), a new antifungal antibiotic, is produced by the myxobacterium myxococcus fulvus strain mx f16. it is active against many filamentous fungi, and completely inhibits growth of mucor hiemalis at a concentration of 2 micrograms/ml. the molecular formula of myxothiazol was determined to e c25h33n3o3s2.19806788741
evidence on intracellular sites of terminal synthesis of carotenoids in mucor hiemalis wehm. 19676036891
a novel disaccharide substrate having 1,2-oxazoline moiety for detection of transglycosylating activity of endoglycosidases.a disaccharide substrate of manbeta1-4glcnac-oxazoline 2 was designed and synthesized as a novel probe for detection of the transglycosylating activity of endoglycosidases. a regio- and stereoselective transglycosylation reaction of 2 to glcnacbeta1-o-pnp or dns-asn(glcnac)-oh catalyzed by endo-beta-n-acetylglucosaminidase from mucor hiemalis (endo-m) and endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) has been demonstrated for the first time, resulting in the core tri ...200111514092
[demonstration of trans isomers of phytoene and phytofluene in rhodopseudomonas spheroides, rhodospirillum rubrum and mucor hiemalis]. 19724622087
myxothiazol, a new antibiotic interfering with respiration.myxothiazol, a new antibiotic from the myxobacterium myxococcus fulvus, inhibited the growth of many yeasts and fungi at concentrations between 0.01 and 3 micrograms/ml. it was generally inactive against bacteria. the inhibitory effect was cytostatic. with candida albicans, saccharomyces cerevisiae, and mucor hiemalis, the growth inhibition was neutralized by glucose. soon after being added to a cell suspension, the compound almost completely blocked oxygen consumption.19817247372
mitosis in mucor hiemalis. a comparative light and electron microscopical study. 19734778923
sexuality in an azygosporic strain of mucor hiemalis. i. breakdown of the azygosporic component. 19665926562
the localization of some enzymes in the mycelium of mucor hiemalis. 19685709638
fatal rhinocerebral mucormycosis in newly diagnosed diabetic.a 48-year-old male developed fever and sore throat while in spain and was admitted to hospital, dehydrated, ketotic, pyrexial, and with a blood glucose of 35 mmol/l. despite treatment with intravenous fluids, insulin, cephalosporin, potassium and bicarbonate he returned to britain 7 days later, underhydrated and acidotic, though euglycaemic. his face was discoloured, there was painless nasal and maxillary swelling, oral candidiasis, and he went on to develop ophthalmoplegia and sudden blindness. ...19862951184
transglycosylation activity of mucor hiemalis endo-beta-n-acetyl-glucosaminidase which transfers complex oligosaccharides to the n-acetylglucosamine moieties of peptides.a novel endo-beta-n-acetylglucosaminidase in the culture fluid of mucor hiemalis isolated from soil was found to have transglycosylation activity. this endo-beta-n-acetylglucosaminidase, endo-m, could liberate the complex type of asparagine-linked oligosaccharides by hydrolysis of diacetylchitobiose linkage from glycoproteins. the treatment of endo-m with n-acetyl-glucosamine and asialotransferrin glycopeptide having the complex type of oligosaccharides resulted in the transfer of the released o ...19948074662
the mineral nutritional requirements of mucor hiemalis wehm. 195413130824
the relative ability of fungi from sphagnum fuscum to decompose selected carbon substrates.nine species from a suite of 55 microfungi isolated from living and decomposing sphagnum fuscum were selected for studies of in vitro decomposition of tannic acid, cellulose, and starch. in vitro decomposition of s. fuscum plants and spruce wood chips was also examined. oidiodendron maius and oidiodendron scytaloides degraded tannic acid, giving a positive reaction for polyphenol oxidases. most taxa degraded cellulose and starch via the synthesis of cellulases and amylase, respectively. mass los ...200211989764
[detection of 6-methylaminopurine in dna of the fungus mucor hiemalis]. 19705498700
lactobacillus coryniformis subsp. coryniformis strain si3 produces a broad-spectrum proteinaceous antifungal compound.the antifungal activity spectrum of lactobacillus coryniformis subsp. coryniformis strain si3 was investigated. the strain had strong inhibitory activity in dual-culture agar plate assays against the molds aspergillus fumigatus, a. nidulans, penicillium roqueforti, mucor hiemalis, talaromyces flavus, fusarium poae, f. graminearum, f. culmorum, and f. sporotrichoides. a weaker activity was observed against the yeasts debaryomyces hansenii, kluyveromyces marxianus, and saccharomyces cerevisiae. th ...200111133421
novel specificities of mucor hiemalis endo-beta-n-acetylglucosaminidase acting complex asparagine-linked oligosaccharides.mucor hiemalis endo-beta-n-acetylglucosaminidase (endo-m) was proved to act on complex type biantennary oligosaccharides of glycoproteins by using dansylated asparagine-linked and pyridylaminated oligosaccharides, as the substrate. the enzyme could act on both asialo- and sialo-biantennary oligosaccharides. this is the only endo-beta-n-acetylglucosaminidase known to act on sialo glycans, though their activity for them was weak. the enzyme could liberate complex type biantennary oligosaccharides ...19947509658
[effect of pantothenic acid, pantethine and phosphorylated pantethine on carotene formation in mucor hiemalis]. 195413210343
microbial endoglycosidases for analyses of oligosaccharide chains in glycoproteins.microbial endoglycosidases are useful for elucidating the structure and function of the oligosaccharide chains of glycoconjugates. most of the microbial endo-beta-n-acetylglucosaminidases including endo-h can preferentially act on high-mannose type chains of asparagine-linked oligosaccharides of various glycoproteins. among them, flavobacterium sp. enzyme is produced in large amounts by the inducing cultivation. using this enzyme, the role of oligosaccharide chains in various microbial glycoenzy ...19947822234
[effect of ecological factors on spore germination and the viability of the mycelial fragments of microscopic fungi].spore germination and the viability of the mycelial fragments of the microscopic fungi alternaria alternata, penicillium spinulosum, and mucor hiemalis were studied with respect to the action of some ecological factors: sucrose concentration (0, 0.2, 2, 10, and 100 g/l), temperature (4, 20, 25, and 30 degrees c), ph (3.5, 4.0, 5.0, 6.2, and 7.0), and cadmium concentration (0, 2, 10, and 100 mg/l). the spore germination rate and the viabilities of different mycelial fragments were found to reach ...200111386056
coprophilous fungi of the horse.a total of 1267 microfungi, including 35 myxomycetes, were recorded from the fecal samples of the 60 horses; of these 395 were found on 20 saddle-horse feces, 363 on 20 race-horses and 509 on 20 working horses. eighty two species representing 53 genera were recorded; of these 7 were zygomycetes, 18 ascomycetes, 1 basidiomycetes and 25 fungi imperfecti: 2 myxomycetes. common coprophilous fungi are in decreasing order pilobolus kleinii, saccobolus depauperatus, mucor hiemalis, lasiobolus ciliatus, ...19817242651
photochemical interaction of dictamnine, a furoquinoline alkaloid, with fungal dna.the furoquinoline alkaloid dictamnine has been shown to provoke lethal damage to filamentous fungi in near ultraviolet light. the phototoxicity was more pronounced against mucor hiemalis and mucor ramannianus than against fusarium graminearum and penicillium italicum. in vitro, labeled dictamnine was shown to form covalent monoadducts with purified dna from m. hiemalis in the presence of long-wave ultraviolet light. addition of [3h]dictamnine to cultures of the same organism showed photobinding ...19827104872
chemo-enzymatic synthesis of a bioactive peptide containing a glutamine-linked oligosaccharide and its characterization.a bioactive peptide containing a glutamine-linked oligosaccharide was chemo-enzymatically synthesized by use of the solid-phase method of peptide synthesis and the transglycosylation activity of endo-beta-n-acetylglucosaminidase. substance p, a neuropeptide, is an undecapeptide containing two l-glutamine residues. a substance p derivative with an n-acetyl-d-glucosamine residue attached to the fifth or sixth l-glutamine residue from the n-terminal region was chemically synthesized. a sialo comple ...200111410333
biological interaction between soil fungi and toxocara canis eggs.fungi from the soil of public areas in la plata, argentina were isolated and evaluated for their biological interaction with toxocara canis eggs in vitro. we isolated and identified two fungal species: fusarium pallidoroseum and mucor hiemalis. each species was co-cultured with t. canis eggs in sterile distilled water. the samples were observed by light and scanning electron microscopy (sem) at days 4, 7 and 14 post-inoculation. under the conditions of our experiments, f. pallidoroseum exhibited ...200211750118
[characteristics of lipid composition in arthrospores, budding cells and mycelium of the fungus mucor hiemalis].the fungus mucor hiemalis f-1156, which is believed to be monomorphic, was found to be able to grow dimorphically in a liquid medium that is free of chemical agents influencing morphogenesis. the growing mycelium produced arthrospores in large amounts. the lipids of the mycelium, yeastlike budding cells, and arthrospores differed in the contents of saturated and unsaturated fatty acids and in the proportion of polar and neutral lipids. the arthrospores contained more monoenoic fatty acids in the ...200111558271
[composition of lipids from yeast-like and mycelial cells of the fungus mucor hiemalis, grown in the presence of 4-chloroaniline].the fungus mucor hiemalis, which is commonly thought to be monomorphic, produced two types of cells, yeastlike and mycelial, during growth in a medium containing 4-chloroaniline. among the polar lipids of yeastlike cells, diphosphatidylglycerol was dominant, while phosphatidylcholine and phosphatidylethanolamine were present in minor amounts. conversely, mycelial cells mainly contained phosphatidylcholine and phosphatidylethanolamine, whereas the content of diphosphatidylglycerol was low. the ne ...200011195578
chemo-enzymatic synthesis of the glycosylated alpha-mating factor of saccharomyces cerevisiae and analysis of its biological activity.the effect of glycosylation on a bioactive peptide was studied using yeast saccharomyces cerevisiae alpha-mating factor, which is composed of 13 amino acids. in this study, we prepared glycosylated alpha-mating factor by chemo-enzymatic synthesis. at first, n-acetylglucosaminyl alpha-mating factor (trp-his-trp-leu-gln(glcnac)-leu-lys-pro-gly-gln-pro-met-tyr) was chemically synthesized by the solid-phase method. then, using the transglycosylation activity of mucor hiemalis endo-beta-n-acetylgluco ...200212234498
[characterization of fungi isolated from woody-chip piles, especially thermophilic and thermotolerant isolates].samples were taken in order to isolate fungi from four woodychip piles (three of these were treated with liquid manure) from different depths. 39 species of 121 isolates could be determined. they represent 28 genera. aspergillus fumigatus, trichoderma reseei, corynascus sespedonium and mucor hiemalis were isolated most frequently. the isolates were examined for their temperature requirements. following the definitions of hawskworth (1983) they could be classified into the three groups of "thermo ...19948087476
growth of chitinolytic dune soil beta-subclass proteobacteria in response to invading fungal hyphae.it has frequently been reported that chitinolytic soil bacteria, in particular biocontrol strains, can lyse living fungal hyphae, thereby releasing potential growth substrate. however, the conditions used in such assays (high bacterial density, rich media, fragmented hyphae) make it difficult to determine whether mycolytic activity is actually of importance for the growth and survival of chitinolytic bacteria in soils. an unidentified group of beta-subclass proteobacteria (cbetaps) was most domi ...200111472904
linkage of sugar chains to a fragment peptide of fgf-5s by a chemoenzymatic strategy and changes in the rate of proteolytic hydrolysis.various o-linked and n-linked sugar chains were linked enzymatically to a fragment peptide (leu-ser-gln(or asn)-val-his-arg) of fgf-5s. first, galactose was linked with beta-(1-->3)-linkage to galnac-linked peptide by a transglycosylation using beta-galactosidase from bacillus circulans (recombinant). then sialic acid was linked with the aid of sialyltransferase from rat liver (recombinant) to give neuacalpha-(2-->3)-galbeta-(1-->3)-galnac-linked hexapeptide. further, a sialylated 2-chain biante ...200111788798
chemoenzymatic synthesis of neoglycopeptides using endo beta-n-acetylglucosaminidase from mucor hiemalis. 200312968358
in vitro evidence of mycoparasitism of the ectomycorrhizal fungus laccaria laccata against mucor hiemalis in the rhizosphere of pinus sylvestris.interactions between the ectomycorrhizal fungus laccaria laccata and the soil fungus mucor hiemalis f. hiemalis in co-culture, and in the rhizosphere of in vitro-grown pinus sylvestris seedlings were investigated by light- and scanning electron-microscopy. in co-culture, mycelial growth away from the l. laccata colony reduced the number of aerial hyphae at the contact zone and increased the density and compactness of the mycelium-characterized gross morphology of the saprobic fungus. although th ...200312634918
transglycosylation of intact sialo complex-type oligosaccharides to the n-acetylglucosamine moieties of glycopeptides by mucor hiemalis endo-beta-n-acetylglucosaminidase.the endo-beta-n-acetylglucosaminidase (endo-beta-glcnac-ase) of mucor hiemalis, endo-m, was found to transfer the sialo complex-type oligosaccharides from transferrin glycopeptide to the n-acetylglucosamine (glcnac) moieties of peptidyl-glcnac. disialo complex-type oligosaccharide of transferrin glycopeptide was transferred to 9-fluorenylmethyloxycarbonyl (fmoc)-asparaginyl-n-acetylglucosaminide (fmoc-asn-glcnac) by endo-m in a high yield. the structure of the reaction product was confirmed to b ...19968870237
identification of an endo-beta-n-acetylglucosaminidase gene in caenorhabditis elegans and its expression in escherichia coli.we report the identification, molecular cloning, and characterization of an endo-beta-n-acetylglucosaminidase from the nematode caenorhabditis elegans. a search of the c. elegans genome database revealed the existence of a gene exhibiting 34% identity to mucor hiemalis (a fungus) endo-beta-n-acetylglucosaminidase (endo-m). actually, the c. elegans extract contained endo-beta-n-acetylglucosaminidase activity. the putative cdna for the c. elegans endo-beta-n-acetylglucosaminidase (endo-ce) was amp ...200212244070
chemo-enzymatic synthesis of eel calcitonin glycosylated at two sites with the same and different carbohydrate structures.naturally occurring glycopeptides and glycoproteins usually contain more than one glycosylation site, and the structure of the carbohydrate attached is often different from site to site. therefore, synthetic methods for preparing peptides and proteins that are glycosylated at multiple sites, possibly with different carbohydrate structures, are needed. here, we report a chemo-enzymatic approach for accomplishing this. complex-type oligosaccharides were introduced to the calcitonin derivatives tha ...200616343462
[composition of sterols from arthrospores and mycelia of the fungus mucor hiemalis].sterol composition of the arthrospores and mycelium of the fungus mucor hiemalis 1156 was studied by the method of chromatography-mass spectrometry. along with ergosterol, the major sterol of the culture studied, ten minor sterol were identified, which were either precursors or products of ergosterol degradation. the content of individual sterols differed substantially in arthrospores and mycelium, which represent different stages of ontogenetic development of the fungus. in arthrospores, the co ...200212244716
microbial models of mammalian metabolism. fungal metabolism of phenolic and nonphenolic p-cymene-related drugs and prodrugs. i. metabolites of thymoxamine.this study was undertaken to validate the use of microbial biotransformation systems for drug metabolism studies. thymoxamine 1 was rapidly hydrolyzed to desacetylthymoxamine (dat) 2 by numerous fungi. other known animal metabolites, such as n-desmethyl-desacetylthymoxamine 3 and desacetylthymoxamine-o-sulfate 6, were produced from dat by mucor rouxii and mortierella isabellina. dat-n-oxide 5, a putative animal microsomal metabolite, was also produced by m. isabellina, in addition, a few strains ...19979172947
survey of mycoflora and mycotoxins in egyptian soybean seeds.after four months in commercial storage, 100 soybean samples from different places of egyptian governorates were assayed for filamentous fungal growth at two incubation temperatures (28 and 45 degrees c). 73 species and 8 varieties belonging to 32 genera were isolated by the dilution plate method. at 28 degrees c, the common species were aspergillus flavus, a. fumigatus, a. niger and a. alutaceus, followed by a. terreus, penicillium chrysogenum, p. citrinum, mucor hiemalis, m. racemosus, emerice ...19938271157
studies on shared antigenic/allergenic components among fungi.fungal allergens have been found to be one of the most prevalent aeroallergens in india. knowledge of shared/unique components among different fungi is necessary for proper diagnosis and treatment of patients allergic to fungi. in the present study, crude extracts (ce) of 11 common fungi (alternaria alternata, aspergillus flavus, asp. fumigatus, asp. niger, asp. tamarii, asp. versicolor, cladosporium herbarum, curvularia lunata, mucor hiemalis, penicillium citrinum, and fusarium solani) were cha ...19958607563
microbial metabolism of 2-arylpropionic acids: chiral inversion of ibuprofen and 2-phenylpropionic acid.the metabolism of (r,s)-ibuprofen has been investigated in 24 microbial cultures. of these cunninghamella elegans, mucor hiemalis, and verticillium lecanii catalyzed the oxidation of the drug to 2-[4-(2-hydroxy-2-methylpropyl)phenyl]propionic acid, a known mammalian metabolite. the extent of metabolism was greatest with v. lecanii, with some 47% of the substrate being consumed over a 7-day incubation period. enantiomeric analysis indicated stereoselective metabolism of (r)-ibuprofen, the enantio ...19938305287
streptomyces halstedii k122 produces the antifungal compounds bafilomycin b1 and c1.streptomyces halstedii k122 was previously found to produce antifungal compounds on solid substrates that inhibit radial growth of fungi among ascomycetes, basidiomycetes, deuteromycetes, oomycetes, and zygomycetes, and strongly affected hyphal branching and morphology. during growth of s. halstedii k122 in submerged culture, no antifungal activity could be detected. however, cultivation of s. halstedii in thin (1 mm) liquid substrate layers in large surface-area tissue culture flasks caused int ...200010941524
epothilons a and b: antifungal and cytotoxic compounds from sorangium cellulosum (myxobacteria). production, physico-chemical and biological properties.an antifungal activity against mucor hiemalis was detected in the culture broth of sorangium cellulosum (myxococcales) strain so ce90. the activity was excreted into the supernatant during the log and early stationary phase. when the adsorber resin xad-16 was added to the culture, the active metabolites were quantitatively bound to the resin. the epothilons showed a high cytotoxicity for animal cells and mimic the biological effects of taxol (bollag et al., cancer res. 55: 2325 approximately 233 ...19968698639
chemoenzymatic synthesis of a novel glycopeptide using a microbial endoglycosidase.the chemoenzymatic synthesis of a glycopeptide by chemical synthesis of n-acetylglucosaminyl peptide and enzymatic transfer of an oligosaccharide is described. we synthesized glycosylated peptide t which blocks infection of human t cells by human immunodeficiency virus. the first step of the chemoenzymatic method is the solid-phase chemical synthesis of n-acetylglucosaminyl peptide t (ala-ser-thr-thr-thr-asn(glcnac)-tyr-thr) with an n-acetylglucosamine moiety bound to the asparaginyl residue by ...19979648260
high efficiency of transferring a native sugar chain from a glycopeptide by a microbial endoglycosidase in organic solvents.we examined the transglycosylation reaction by the recombinant endo-beta-n-acetylglucosaminidase from mucor hiemalis (endo-m) expressed in candida boidinii in media containing organic solvents. the recombinant endo-m could transglycosylate a disialo biantennary complex-type oligosaccharide from hen egg yolk glycopeptide to p-nitrophenyl n-acetyl-beta-d-glucosaminide even in the presence of 30% acetone, dimethyl sulfoxide, or methanol. the yield of the transglycosylation product reached 21-34% of ...200415013411
the structure and behavior of the nuclei in spores and growing hyphae of mucorales. i. mucor hiemalis and mucor fragilis. 195713460827
newly discovered neutral glycosphingolipids in aureobasidin a-resistant zygomycetes: identification of a novel family of gala-series glycolipids with core gal alpha 1-6gal beta 1-6gal beta sequences.we found for the first time that zygomycetes species showed resistance to aureobasidin a, an antifungal agent. a novel family of neutral glycosphingolipids (gsls) was found in these fungi and isolated from mucor hiemalis, which is a typical zygomycetes species. their structures were completely determined by compositional sugar, fatty acid, and sphingoid analyses, methylation analysis, matrix-assisted laser desorption ionization time-of-flight/mass spectrometry, and (1)h nmr spectroscopy. they we ...200415155728
chemo-enzymatic synthesis of calcitonin derivatives containing n-linked oligosaccharides.eel calcitonin derivatives containing various n-linked oligosaccharides were chemo-enzymatically synthesized by the transglycosylation reaction of mucor hiemalis endo-beta-n-acetylglucosaminidase (endo-m) to a glycosylated calcitonin derivative [asn(glcnac)3]-ct in which n-acetyl-d-glycosamine (glcnac) is attached to the l-asparagine (asn) residue of the peptide.19989871755
[changes in the lipid composition of mucor hiemalis sporangiospores related to the age of the spore-forming culture].analysis of sporangiospore lipids of the fungus mucor hiemalis f-1156 showed that alterations occur in the content of fatty acids and individual classes of lipids during long-term cultivation (for about 20 days). the changes in the lipid composition related to the age of the spore-forming mycelium suggest an important role of sporangiospore lipids in spore germination and in further development of the spherical cells formed in this processes. the m. hiemalis f-1156 sporangiospores with a lipid p ...200314526543
transglycosylation reaction of mucor hiemalis endo-beta-n-acetylglucosaminidase using sugar derivatives modified at c-1 or c-2 as oligosaccharide acceptors.we investigated the transglycosylation reaction of the recombinant endo-beta-n-acetylglucosaminidase from mucor hiemalis (endo-m) expressed in candida boidinii using such sugar derivatives as n-acylated d-glucosamines, c-glucosyl derivatives, and a 2-o-glycosylated disaccharide as acceptors. we found that a variety of sugar derivatives modified at c-1 or c-2 could be used as acceptors for transglycosylation by endo-m to create novel oligosaccharides.200415113683
structural and functional characterization of ovotransferrin produced by pichia pastoris.ovotransferrin is an egg white protein with complex disulfide and bilobal structures, which is derived from the same gene as chicken serum transferrin. we demonstrate here the structural and functional characteristics of bilobal ovotransferrin, produced at a high level using pichia pastoris expression system. the recombinant protein was secreted into the medium, and the secretion signal peptide was processed correctly. the secretion level was almost 100 mg/l culture and the yield after purificat ...200414981301
enhancement of bioactivity of saccharomyces cerevisiae alpha-mating factor by attachment of sugar moiety to glutamine residue.we prepared yeast saccharomyces cerevisiae alpha-mating factor, a 13-amino acid pheromone produced by haploid alpha-cells, bound with glucose or n-acetylglucosamine at the fifth glutamine residue from the n-terminal by the chemical method of peptide synthesis. it was found that the bioactivity of glucosyl alpha-mating factor was higher than that of native alpha-mating factor. however, it was slightly lower than that of n-acetylglucosaminyl alpha-mating factor. this suggested that the n-acetylami ...200415522439
[the presence of non-carotinoid yellow pigments in mucor hiemalis]. 195214945437
[a possible pathway in the biosynthesis of carotenoids in mucor hiemalis]. 195513241486
synthesis of mono-glucose-branched cyclodextrins with a high inclusion ability for doxorubicin and their efficient glycosylation using mucor hiemalis endo-beta-n-acetylglucosaminidase.the mono-glucose-branched cyclodextrins having an appropriate spacer between the beta-cyclodextrin and a glucose moiety were synthesized from beta-cyclodextrin and arbutin. they had the significantly high association constants for doxorubicin, the anticancer agent, in the range of 10(5)-10(6)m(-1), and worked as highly reactive glycosyl acceptors for the transglycosylation reaction by endo-beta-n-acetylglucosaminidase of mucor hiemalis to produce sialo-complex type oligosaccharide-branched cyclo ...200515686902
bioactive sesquiterpene lactones from the leaves of vernonia amygdalina.phytochemical analysis of the leaves of vernonia amygdalina yielded two known sesquiterpene lactones: vernolide and vernodalol. the two compounds were tested by agar dilution method against 10 bacteria strains and 5 fungi species. both compounds exhibited a significant bactericidal activity against five gram positive bacteria while lacking efficacy against the gram negative strains. in the antifungal test, while vernolides exhibited high activity with lc(50) values of 0.2, 0.3 and 0.4 mg/ml agai ...200616458461
evaluation of solid-state bioconversion of domestic wastewater sludge as a promising environmental-friendly disposal technique.natural and environmental-friendly disposal of wastewater sludge is a great concern. recently, biological treatment has played prominent roles in bioremediation of complex hydrocarbon- rich contaminants. composting is quite an old biological-based process that is being practiced but it could not create a great impact in the minds of concerned researchers. the present study was conducted to evaluate the feasibility of the solid-state bioconversion (ssb) processes in the biodegradation of wastewat ...200415491662
[sterols of the fungus mucor hiemalis sporangiospores].we earlier reported that the viability and lipid composition of mucor hiemalis f-1156 sporangiospores, as well as their capacity to develop yeast-like cells, depend on the age of the spore-forming culture [1]. with the increase in the time of the culture growth, sporangiospores exhibit a decrease in the levels of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, cardiolipin, and reserve lipids, as well as in the degree of fatty acid unsaturation, whereas the level of glycolipids ...200314768556
mucor hiemalis endo-beta-n-acetylglucosaminidase can transglycosylate a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide.we found that the recombinant endo-beta-n-acetylglucosaminidase of mucor hiemalis (endo-m) expressed in candida boidinii had the transglycosylation activity of transferring a bisecting hybrid-type oligosaccharide from an ovalbumin glycopeptide to the acceptor (p-nitrophenyl 2-acetamido-2-deoxy-beta-d-glucopyranoside) in a good yield of 43%.200415476727
[biosynthesis of beta-carotene in mucor hiemalis; role of acetic acid in, the structure of carotene molecule studies with radiocarbon-tagged acetic acid]. 195413183060
a remodeling system for the oligosaccharide chains on glycoproteins with microbial endo-beta-n-acetylglucosaminidases.endo-m, endo-beta-n-acetylglucosaminidase from mucor hiemalis, transferred the complex type oligosaccharide of sialoglycopeptide to partially deglycosylated proteins (n-acetylglucosamine-attached proteins), which were prepared by excluding high-mannose type oligosaccharides from glycoproteins with endo-h, endo-beta-n-acetylglucosaminidase from streptomyces plicatus. this finding indicated that the high-mannose type oligosaccharides on glycoproteins can be changed to complex type ones by the tran ...200617049165
partial purification and insecticidal activity of toxic metabolites secreted by a mucor hiemalis strain (smu-21) against adults of bactrocera oleae and ceratitis capitata (diptera: tephritidae).the secondary metabolites present in the methanol extract of a mucor hiemalis strain (smu-21) mycelia, cultured in liquid medium, were evaluated for toxicity to bactrocera oleae (gmelin) and ceratitis capitata (wiedemann) (diptera: tephritidae) adults. feeding and contact bioassays revealed that the methanol fraction of the crude supernatant was strongly toxic to both species. symptoms of toxicity developed quickly; lethargy occurred 1-2 h posttreatment, and mortality reached 82-97% after 24 h. ...200617066796
study of cellulolytic soil fungi and two nova species and new medium.this study is aimed at identifying and determining the percentage of occurrence frequency of cellulose decomposing soil fungi. the soil samples were inoculated into culture plates prepared in sabouraud medium under sterilized conditions and incubated at 30 degrees c for 4 to 7 d. the identified fungal species were incubated in self-designed cellulose medium for testing their cellulolytic ability. forty-two species, including 2 nova species, representing sixteen genera showed growth and sporulati ...200616691640
chemo-enzymatic synthesis and structure-activity study of artificially n-glycosylated eel calcitonin derivatives with a complex type oligosaccharide.starting from n-glycosylated eel calcitonin derivatives that contain an n-acetyl-d-glucosamine residue specifically at the 3rd, 14th, 20th or 26th amino acid residue, corresponding glycopeptides with a complex-type oligosaccharide attached to the respective amino acid residue were synthesized by means of a transglycosylation reaction catalyzed by an endo-beta-n-acetylglucosaminidase from mucor hiemalis . the use of a recombinant enzyme and an excess of a glycosyl donor led to a yield in excess o ...200415514484
molecular cloning of mucor hiemalis endo-beta-n-acetylglucosaminidase and some properties of the recombinant enzyme.endo-m, endo-beta-n-acetylglucosaminidase from mucor hiemalis, is known as a useful enzyme for the synthesis of neoglycopeptides due to its transglycosylation activity. we cloned the endo-m gene encoding a putative 744 amino acids, which shows high identity to glycoside hydrolase family 85 endo-beta-n-acetylglucosaminidases. the gene encoding endo-m was expressed in protease-deficient candida boidinii with a molecular mass of 85 kda as a monomeric form. recombinant endo-m could liberate both hig ...200415519295
[the assessment of the sensitivity of environmental fungi to urea phosphate].in this paper the authors presented the influence of various urea phosphate doses, 1-12%, on the environmental fungi growth depending on its intensity and the type of created spore e.g. (penicillium italicum, aspergillusfumigatus, aspergillus niger, cladosporium resinae, mucor hiemalis i rhizopus nigricans). the result of urea phosphate effect on fungi was estimated by static culture method using dry mass increase change [g. dry mass/ldm3]. the control was created by the growth of the above ment ...200415732502
influence of autoclaved saprotrophic fungal mycelia on proteolytic activity in ectomycorrhizal fungi.the production of proteolytic enzymes by several strains of ectomycorrhizal fungi i.e., amanita muscaria (16-3), laccaria laccata (9-12), l. laccata (9-1), suillus bovinus (15-4), suillus bovinus (15-3), suillus luteus (14-7) on mycelia of trichoderma harzianum, trichoderma virens and mucor hiemalis and sodium caseinate, yeast extract was evaluated. the strains of a. muscaria (16-3) and l. laccata (9-12) were characterized by the highest activity of the acidic and neutral proteases. taking the m ...200717333458
studies on mould growth and biomass production using waste banana peel.hyphomycetous (aspergillus fumigatus) and phycomycetous (mucor hiemalis) moulds were cultivated in vitro at room temperature (28 + 20 degrees c) to examined their growth and biomass production on waste banana peel agar (bpa) and broth (bpb) using commercial malt extract agar (mea) and broth (meb) as control. the moulds grew comparatively well on banana peel substrates. no significant difference (p > 0.05) in radial growth rates was observed between moulds cultivated on pba and mea, although grow ...200515939272
chemoenzymatic synthesis and application of a sialoglycopolymer with a chitosan backbone as a potent inhibitor of human influenza virus hemagglutination.sialoglycopeptide (sgp) is referred as the glycopeptide in hen's egg yolk, which has an n-linked, complex-type, disialyl biantennary oligosaccharide with an alpha-(2-->6)-sialyl n-acetyllactosamine residue. the residue is known as a binding ligand of type-a human influenza virus hemagglutinin. we describe herein a simple synthesis of a sialoglycopolymer with a chitosan backbone as a potent inhibitor of human influenza virus hemagglutination that makes use of the natural source ingredient, sgp, a ...200616716273
synthesis of enzymes connected with mycoparasitism by ectomycorrhizal fungi.the production of enzymes involved in mycoparasitism by several strains of ectomycorrhizal fungi: amanita muscaria (16-3), laccaria laccata (9-12), l. laccata (9-1), suillus bovinus (15-4), s. bovinus (15-3), s. luteus (14-7) on different substrates such as colloidal chitin, mycelia of trichoderma harzianum, t. virens and mucor hiemalis was examined. chitinases and beta-1,3-glucanases were assayed spectrophotometrically by measuring the amount of reducing sugars releasing from suitable substrate ...200616395555
[the effect of hydrogen peroxide on the growth of microscopic mycelial fungi isolated from habitats with different levels of radioactive contamination].the effect of hydrogen peroxide ( 10(-9)-10(-1) m) on the mycelial growth of the fungi alternaria alternata, cladosporium cladosporioides, mucor hiemalis, and paecilomyces lilacinus has been studied. the growth of fungi isolated from habitats with a background level of radioactive contamination was stopped by h2o2 concentrations equal to 10(-3) and 10(-2) m, whereas the growth of fungi that were isolated from habitats with high levels of radioactive contamination was only arrested by 10(-1) m h2 ...200516400985
micromycetes, producers of toxins, detected on stored vegetables.in 2003-2004, investigations of mycological contamination of stored and newly harvested vegetables were carried out. the aim of the study was to detect fungal species able to synthesize toxic metabolites, which are spread on vegetables under various conditions. for mycological investigations, samples of carrots, onions and cabbage were taken from storehouses with different storage periods and conditions. penicillium expansum, p. nalgiovense, mucor silvaticus and penicillium verrucosum were more ...200516457482
cutaneous mucormycosis in a young, immunocompetent girl.we report a case of cutaneous mucormycosis in a healthy, immunocompetent young girl (age 14 years). the patient had a 5-year history of a slowly enlarging, erythematous plaque with slight elevated, scaling, circinate borders on the right thigh. histopathology showed a granulomatous infiltrate with broad, pale, non-septate hyphae. mycological study identified mucor hiemalis (wehmer).200616966176
chemoenzymatic synthesis of cd52 glycoproteins carrying native n-glycans.a facile synthesis of homogeneous cd52 glycoproteins carrying native n-glycans was achieved using an endolycosidase-catalyzed oligosaccharide transfer as the key step. the synthesis consists of two steps: the solid phase synthesis of glcnac-cd52 and the transfer of a high-mannose type or complex type n-glycan from man(9)glcnac(2) asn or a sialglycopeptide to the glcnac-cd52, under the catalysis of the endo-beta-n-acetylglucosaminidases from arthrobacter (endo-a) and mucor hiemalis (endo-m), resp ...200515686882
resolution of oligosaccharides in glycopeptides using immobilized endo-m and ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry.the resolution of asparagine-linked oligosaccharides in glycopeptides was carried out by combination of the transglycosylation reaction and ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry (uplc-esi-tof-ms). the resolution of the oligosaccharides is based on the enzymic transglycosylation reaction with endo-beta-n-acetylglucosaminidase (endo-m) isolated from mucor hiemalis. the oligosaccharides were transferred to a fluorescent acceptor (nda-a ...200717441218
lipase induction in mucor hiemalis.the influence on lipase induction in mucor hiemalis of different types of triglycerides containing mainly oleic acid (olive oil), erucic acid (mustard oil), or saturated fatty acids of 8 to 16 carbons (coconut oil) was studied. the fungus was grown in shake flasks in a fermentation medium containing peptone, minerals, and glucose or one of the oils as the carbon source. maximum lipase was produced when the initial ph of the fermentation medium was kept at 4.0. addition of ca to the medium did no ...198016345604
chemo-enzymatic synthesis of bioactive glycopeptide using microbial endoglycosidase.the chemo-enzymatic synthesis of a glycopeptide, which involves the chemical synthesis of n-acetylglucosaminyl peptide and the enzymatic transfer of oligosaccharide, is described. the first step of the chemo-enzymatic method is the chemical synthesis of n-acetylglucosaminyl peptide with an n-acetylglucosamine moiety bound to the asparaginyl residue of the peptide by a solid-phase method. the second step is transglycosylation of a complex-type oligosaccharide derived from a glycopeptide to an n-a ...200116233137
production of bioethanol by direct bioconversion of oil-palm industrial effluent in a stirred-tank bioreactor.the purpose of this study was to evaluate the feasibility of producing bioethanol from palm-oil mill effluent generated by the oil-palm industries through direct bioconversion process. the bioethanol production was carried out through the treatment of compatible mixed cultures such as thrichoderma harzianum, phanerochaete chrysosporium, mucor hiemalis, and yeast, saccharomyces cerevisiae. simultaneous inoculation of t. harzianum and s. cerevisiae was found to be the mixed culture that yielded th ...200919294441
acceptor specificity in the transglycosylation reaction using endo-m.to determine the structural specificity of the glycosyl acceptor of the transglycosylation reaction using endo-β-n-acetylglucosaminidase (engase) (ec 3.2.1.96) from mucor hiemalis (endo-m), several acceptor derivatives were designed and synthesized. the narrow regions of the 1,3-diol structure from the 4- to 6-hydroxy functions of glcnac were found to be essential for the transglycosylation reaction using endo-m. furthermore, it was determined that endo-m strictly recognizes a 1,3-diol structure ...201020934176
glycosynthases enable a highly efficient chemoenzymatic synthesis of n-glycoproteins carrying intact natural n-glycans.homogeneous n-glycoproteins carrying defined natural n-glycans are essential for detailed structural and functional studies. the transglycosylation activity of the endo-beta-n-acetylglucosaminidases from arthrobacter protophormiae (endo-a) and mucor hiemalis (endo-m) holds great potential for glycoprotein synthesis, but the wild-type enzymes are not practical for making glycoproteins carrying native n-glycans because of their predominant activity for product hydrolysis. in this article, we repor ...200919199609
chemo-enzymatic synthesis of glycosylated insulin using a glcnac tag.artificial insulin with an n-linked oligosaccharide was synthesized by a chemo-enzymatic method using endo-beta-n-acetylglucosaminidase from mucor hiemalis (endo-m). glcnac-modified insulin was prepared by the reaction of the carboxymethyl glycoside of glcnac and 3 amino groups of bovine insulin using a dimethylphosphinothioic mixed anhydride (mpt-ma) method. a transglycosylation reaction of the glcnac-modified insulin using endo-m gave mono-transglycosylated insulin predominantly. we determined ...201020036558
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