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effects of oxygen and glucose on energy metabolism and dimorphism of mucor genevensis grown in continuous culture: reversibility of yeast-mycelium conversion.mucor genevensis was grown in both glucose-limited and glucose-excess continuous cultures over a range of dissolved oxygen concentrations (<0.1 to 25 mum) to determine the effects of glucose and the influence of metabolic mode (fermentative versus oxidative) on dimorphic transformations in this organism. the extent of differentiation between yeast and mycelial phases has been correlated with physiological and biochemical parameters of the cultures. under glucose limitation, oxidative metabolism ...19744152122
relationship between dimorphology and respiration in mucor genevensis studied with chloramphenicol.growth of mucor genevensis, a facultatively anaerobic dimorphic mold, in high concentrations of chloramphenicol (4 mg/ml) leads to increased numbers of yeast-like cells and small club-like mycelial forms. this change in morphology is accompanied by a threefold increase in the mass doubling time, the loss of cyanide-sensitive respiration, and the development of cyanide-insensitive respiration. associated with these changes is the absence of cytochromes aa(3) and b and the inability of the organis ...19734745438
oxylipin formation in fungi: biotransformation of arachidonic acid to 3-hydroxy-5,8-tetradecadienoic acid by mucor genevensis.the soil fungus mucor genevensis was shown to convert exogenous arachidonic acid to the oxylipin 3-hydroxy-5z,8z-tetradecadienoic acid (3-htde) as determined by gas chromatography/mass spectrometry. this metabolite was only found in the aqueous supernatant together with free linoleic acid, but not in the final fungal biomass. in contrast, the corresponding primary arachidonic acid metabolite (3r)-hydroxy-(5z,8z,11z,14z)-eicosatetraenoic acid (3-hete), which has been earlier shown to be produced ...19989918790
variability of isozyme and rapd markers among isolates of mucor genevensis.mucor genevensis is a dimorphic and homothallic fungal species (zygomycetes). ten m. genevensis strains, each strain of the recently described new homothallic species (m. meguroense and m. hachijyoensis) and strains of m. hiemalis and m. piriformis (as outgroups for numerical analysis) were investigated. five different enzyme systems (cat, gdh, g6d, mdh and sod) and five 10-bp random primers were used in isoenzyme and random amplified polymorphic dna analyses, respectively. the data from these s ...200111426871
bioprospecting for novel oxylipins in fungi: the presence of 3-hydroxy oxylipins in pilobolus.as previously found in various members of the mucorales, 3-hydroxy oxylipins in mucor genevensis are associated with the sporangia, i.e. mainly the columella structure and between aggregating sporangiospores. to determine if this phenomenon is also true in distantly related members, the mucoralean fungus pilobolus was examined. this fungus is characterized by relatively large sub sporangial-columella structures which actively eject sporangia in a sticky liquid for attachment onto herbage surroun ...200111761371
fungal bioconversion of 2,4-dichlorophenoxyacetic acid (2,4-d) and 2,4-dichlorophenol (2,4-dcp).ninety strains of fungi from the collection of our mycology laboratory were tested in galzy and slonimski (gs) synthetic liquid medium for their ability to degrade the herbicide 2,4-dichlorophenoxyacetic acid (2,4-d) and its by-product, 2,4-dichlorophenol (2,4-dcp) at 100 mg l(-1), each. evolution of the amounts of each chemical in the culture media was monitored by hplc. after 5 days of cultivation, the best results were obtained with aspergillus penicilloides and mortierella isabellina for 2,4 ...200516201028
fatty acid and sterol composition of mucor genevensis in relation to dimorphism and anaerobic growth.fatty acid and sterol content and composition were determined for the dimorphic mold, mucor genevensis, grown under a variety of experimental conditions. fatty acids account for 6 to 9% of the dry weight of aerobically grown mycelium, and 70 to 80% of these are unsaturated. the organism contains gamma-linolenic acid which is characteristic for phycomycetes, and in sporangiospores this compound represents 40% of the total fatty acids. of the sterols found in mycelium, 80% is ergosterol, and stigm ...20074327506
cyclic adenosine 3',5'-monophosphate and germination of sporangiospores from the fungus mucor.cyclic adenosine 3',5'-monophosphate (camp) metabolism was examined in germinating sporangiospores of mucor genevensis and mucor mucedo. exogenous camp prevented normal hyphal development from sporangiospores. internal pools of camp fluctuated profoundly during development. spherical growth of the spores was characterized by large pools of camp whereas germ tube emergence and hyphal elongation were characterized by small pools of camp. these observations suggest a possible role for camp in spora ...19806254459
development of respiration and mitochondria in mucor genevensis after anaerobic growth: absence of glucose repression.respiration and mitochondria in mucor genevensis, a facultatively anaerobic dimorphic mold, have been studied in aerobically and anaerobically grown cells and in anaerobically grown cells adapting to aerobic conditions. respiration in hyphae continues at a high level during aerobic growth but drops rapidly on exhaustion of glucose. in anaerobically grown yeastlike cells, containing no recognizable aerobic cytochromes, a small cyanide-insensitive respiration occurs. mitochondria with well defined ...19724109888
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