| a survey for haemagglutination-inhibiting antibody to west nile virus in human and animal sera in nigeria. | a survey for west nile virus (wnv) haemagglutination-inhibition (hi) antibody was carried out in humans and domestic animals. human sera were collected from ibadan, while the animal sera were collected from both ibadan and maiduguri. out of 304 human sera tested, 123 were positive (40%). there was a higher prevalence of hi antibody in adults than children. sex distribution of positive sera showed that 37% of males and 43% of females had wnv hi antibody. there was no significant difference in the ... | 1990 | 2323182 |
| susceptibility of laboratory and domestic animals to experimental infection with potiskum virus. | the biological characteristics of potiskum virus, a hitherto undescribed virus isolated in nigeria from the liver of a giant rat (cricetomys gambianus), were studied by experimental infections of laboratory and domestic animals. the laboratory animal hosts used included mice, rats, rabbits and chicks. suckling and weaning mice succumbed to fatal infection when infected with potiskum virus by intracerebral or intraperitoneal routes. infected mice had high titres of virus and mild histopathologica ... | 1989 | 2558278 |
| potiskum virus: enhancement of replication in a macrophage-like cell line. | replication of potiskum virus was studied in p388d1 macrophage-like cell line in the presence and absence of subneutralizing concentrations of specific antiviral antibody. the cultures were infected at multiplicities of infection (moi) ranging from 0.4 to 0.0004. the virus replicated to high titres at all moi tested, but there was an enhancement of virus replication in cultures supplemented with the antibody. enhancement of replication was moi dependent, the highest ratios being obtained in cult ... | 1987 | 2894139 |
| cross-infection enhancement among african flaviviruses by immune mouse ascitic fluids. | cross-infection enhancement of seven african flaviviruses by subneutralising concentrations of antibody in immune ascitic fluids was investigated in p388d1 cell culture. infection by all the seven flaviviruses tested was enhanced by homologous and at least one of six heterologous immune mouse ascitic fluids (imaf) tested. enhancement ratios and enhancing antibody titres were higher in homologous than in heterologous enhancement. zika, wesselsbron, uganda s and west nile viruses were enhanced in ... | 1987 | 3028713 |
| antibody-mediated enhancement of wesselsbron virus in p388d1 cells. | antibody-mediated enhancement of wesselsbron virus was investigated in p388d1 cell cultures. virus infection was enhanced in culture by various dilutions of homologous and heterologous flavivirus antibody. highest enhancement ratios and enhancing antibody titres were obtained with the homologous antibody. enhancement of wesselsbron virus infection in p388d1 cultures was also dependent on the multiplicity of infection (moi) used; cultures infected at the lowest moi produced the highest enhancemen ... | 1986 | 3031959 |
| phylogeny of the genus flavivirus. | we undertook a comprehensive phylogenetic study to establish the genetic relationship among the viruses of the genus flavivirus and to compare the classification based on molecular phylogeny with the existing serologic method. by using a combination of quantitative definitions (bootstrap support level and the pairwise nucleotide sequence identity), the viruses could be classified into clusters, clades, and species. our phylogenetic study revealed for the first time that from the putative ancesto ... | 1998 | 9420202 |
| structure and functionality in flavivirus ns-proteins: perspectives for drug design. | flaviviridae are small enveloped viruses hosting a positive-sense single-stranded rna genome. besides yellow fever virus, a landmark case in the history of virology, members of the flavivirus genus, such as west nile virus and dengue virus, are increasingly gaining attention due to their re-emergence and incidence in different areas of the world. additional environmental and demographic considerations suggest that novel or known flaviviruses will continue to emerge in the future. nevertheless, u ... | 2010 | 19945487 |
| structural and functional analyses of a conserved hydrophobic pocket of flavivirus methyltransferase. | the flavivirus methyltransferase (mtase) sequentially methylates the n7 and 2'-o positions of the viral rna cap (gpppa-rna → m(7)gpppa-rna → m(7)gpppam-rna), using s-adenosyl-l-methionine (adomet) as a methyl donor. we report here that sinefungin (sin), an adomet analog, inhibits several flaviviruses through suppression of viral mtase. the crystal structure of west nile virus mtase in complex with sin inhibitor at 2.0-å resolution revealed a flavivirus-conserved hydrophobic pocket located next t ... | 2010 | 20685660 |
| ecuador paraiso escondido virus, a new flavivirus isolated from new world sand flies in ecuador, is the first representative of a novel clade in the genus flavivirus. | a new flavivirus, ecuador paraiso escondido virus (epev), named after the village where it was discovered, was isolated from sand flies (psathyromyia abonnenci, formerly lutzomyia abonnenci) that are unique to the new world. this represents the first sand fly-borne flavivirus identified in the new world. epev exhibited a typical flavivirus genome organization. nevertheless, the maximum pairwise amino acid sequence identity with currently recognized flaviviruses was 52.8%. phylogenetic analysis o ... | 2015 | 26355096 |
| characterization of a novel insect-specific flavivirus from brazil: potential for inhibition of infection of arthropod cells with medically important flaviviruses. | in the past decade, there has been an upsurge in the number of newly described insect-specific flaviviruses isolated pan-globally. we recently described the isolation of a novel flavivirus (tentatively designated 'nhumirim virus'; nhuv) that represents an example of a unique subset of apparently insect-specific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses despite appearing to be limited to replication in mosquito cells. we characterized the in vitro growth p ... | 2014 | 25146007 |
| evaluation of a field-portable dna microarray platform and nucleic acid amplification strategies for the detection of arboviruses, arthropods, and bloodmeals. | highly multiplexed assays, such as microarrays, can benefit arbovirus surveillance by allowing researchers to screen for hundreds of targets at once. we evaluated amplification strategies and the practicality of a portable dna microarray platform to analyze virus-infected mosquitoes. the prototype microarray design used here targeted the non-structural protein 5, ribosomal rna, and cytochrome b genes for the detection of flaviviruses, mosquitoes, and bloodmeals, respectively. we identified 13 of ... | 2012 | 23249687 |
| flavidb: a data mining system for knowledge discovery in flaviviruses with direct applications in immunology and vaccinology. | the flavivirus genus is unusually large, comprising more than 70 species, of which more than half are known human pathogens. it includes a set of clinically relevant infectious agents such as dengue, west nile, yellow fever, and japanese encephalitis viruses. although these pathogens have been studied extensively, safe and efficient vaccines lack for the majority of the flaviviruses. | 0 | 25544857 |