| novel variant of tickborne encephalitis virus, russia. | we isolated a novel strain of tickborne encephalitis virus (tbev), glubinnoe/2004, from a patient with a fatal case in russia. we sequenced the strain, whose landmark features included 57 amino acid substitutions and 5 modified cleavage sites. phylogenetically, glubinnoe/2004 is a novel variant that belongs to the eastern type of tbev. | 2007 | 18258012 |
| Tembusu virus in ducks, china. | In China in 2010, a disease outbreak in egg-laying ducks was associated with a flavivirus. The virus was isolated and partially sequenced. The isolate exhibited 87%-91% identity with strains of Tembusu virus, a mosquito-borne flavivirus of the Ntaya virus group. These findings demonstrate emergence of Tembusu virus in ducks. | 2011 | 22000358 |
| revisiting the clinal concept of evolution and dispersal for the tick-borne flaviviruses by using phylogenetic and biogeographic analyses. | tick-borne flaviviruses (tbf) are widely dispersed across africa, europe, asia, oceania, and north america, and some present a significant threat to human health. seminal studies on tick-borne encephalitis viruses (tbev), based on partial envelope gene sequences, predicted a westward clinal pattern of evolution and dispersal across northern eurasia, terminating in the british isles. we tested this hypothesis using all available full-length open reading frame (orf) tbf sequences. phylogenetic ana ... | 2012 | 22674986 |
| preparing for the next epidemic with basic virology. | | 2015 | 26492495 |
| biological transmission of arboviruses: reexamination of and new insights into components, mechanisms, and unique traits as well as their evolutionary trends. | among animal viruses, arboviruses are unique in that they depend on arthropod vectors for transmission. field research and laboratory investigations related to the three components of this unique mode of transmission, virus, vector, and vertebrate host, have produced an enormous amount of valuable information that may be found in numerous publications. however, despite many reviews on specific viruses, diseases, or interests, a systematic approach to organizing the available information on all f ... | 0 | 16223950 |
| ecuador paraiso escondido virus, a new flavivirus isolated from new world sand flies in ecuador, is the first representative of a novel clade in the genus flavivirus. | a new flavivirus, ecuador paraiso escondido virus (epev), named after the village where it was discovered, was isolated from sand flies (psathyromyia abonnenci, formerly lutzomyia abonnenci) that are unique to the new world. this represents the first sand fly-borne flavivirus identified in the new world. epev exhibited a typical flavivirus genome organization. nevertheless, the maximum pairwise amino acid sequence identity with currently recognized flaviviruses was 52.8%. phylogenetic analysis o ... | 2015 | 26355096 |
| flavidb: a data mining system for knowledge discovery in flaviviruses with direct applications in immunology and vaccinology. | the flavivirus genus is unusually large, comprising more than 70 species, of which more than half are known human pathogens. it includes a set of clinically relevant infectious agents such as dengue, west nile, yellow fever, and japanese encephalitis viruses. although these pathogens have been studied extensively, safe and efficient vaccines lack for the majority of the flaviviruses. | 0 | 25544857 |
| experimental transmission of karshi (mammalian tick-borne flavivirus group) virus by ornithodoros ticks >2,900 days after initial virus exposure supports the role of soft ticks as a long-term maintenance mechanism for certain flaviviruses. | members of the mammalian tick-borne flavivirus group, including tick-borne encephalitis virus, are responsible for at least 10,000 clinical cases of tick-borne encephalitis each year. to attempt to explain the long-term maintenance of members of this group, we followed ornithodoros parkeri, o. sonrai, and o. tartakovskyi for >2,900 days after they had been exposed to karshi virus, a member of the mammalian tick-borne flavivirus group. | 2015 | 26285211 |
| structural and functional analyses of a conserved hydrophobic pocket of flavivirus methyltransferase. | the flavivirus methyltransferase (mtase) sequentially methylates the n7 and 2'-o positions of the viral rna cap (gpppa-rna → m(7)gpppa-rna → m(7)gpppam-rna), using s-adenosyl-l-methionine (adomet) as a methyl donor. we report here that sinefungin (sin), an adomet analog, inhibits several flaviviruses through suppression of viral mtase. the crystal structure of west nile virus mtase in complex with sin inhibitor at 2.0-å resolution revealed a flavivirus-conserved hydrophobic pocket located next t ... | 2010 | 20685660 |
| ticks associated with macquarie island penguins carry arboviruses from four genera. | macquarie island, a small subantarctic island, is home to rockhopper, royal and king penguins, which are often infested with the globally distributed seabird tick, ixodes uriae. a flavivirus, an orbivirus, a phlebovirus, and a nairovirus were isolated from these ticks and partial sequences obtained. the flavivirus was nearly identical to gadgets gully virus, isolated some 30 year previously, illustrating the remarkable genetic stability of this virus. the nearest relative to the orbivirus (for w ... | 2009 | 19194498 |
| structural bases for substrate recognition and activity in meaban virus nucleoside-2'-o-methyltransferase. | viral methyltransferases are involved in the mrna capping process, resulting in the transfer of a methyl group from s-adenosyl-l-methionine to capped rna. two groups of methyltransferases (mtases) are known: (guanine-n7)-methyltransferases (n7mtases), adding a methyl group onto the n7 atom of guanine, and (nucleoside-2'-o-)-methyltransferases (2'omtases), adding a methyl group to a ribose hydroxyl. we have expressed and purified two constructs of meaban virus (mv; genus flavivirus) ns5 protein m ... | 2007 | 17473012 |
| phylogeny of the genus flavivirus. | we undertook a comprehensive phylogenetic study to establish the genetic relationship among the viruses of the genus flavivirus and to compare the classification based on molecular phylogeny with the existing serologic method. by using a combination of quantitative definitions (bootstrap support level and the pairwise nucleotide sequence identity), the viruses could be classified into clusters, clades, and species. our phylogenetic study revealed for the first time that from the putative ancesto ... | 1998 | 9420202 |
| circumpolar diversification of the ixodes uriae tick virome. | ticks (order: ixodida) are a highly diverse and ecologically important group of ectoparasitic blood-feeding organisms. one such species, the seabird tick (ixodes uriae), is widely distributed around the circumpolar regions of the northern and southern hemispheres. it has been suggested that ix. uriae spread from the southern to the northern circumpolar region millions of years ago and has remained isolated in these regions ever since. such a profound biographic subdivision provides a unique oppo ... | 2020 | 32745135 |
| first dating of a recombination event in mammalian tick-borne flaviviruses. | the mammalian tick-borne flavivirus group (mtbfg) contains viruses associated with important human and animal diseases such as encephalitis and hemorrhagic fever. in contrast to mosquito-borne flaviviruses where recombination events are frequent, the evolutionary dynamic within the mtbfg was believed to be essentially clonal. this assumption was challenged with the recent report of several homologous recombinations within the tick-borne encephalitis virus (tbev). we performed a thorough analysis ... | 2012 | 22384119 |
| inhibition of protein kinase c promotes dengue virus replication. | dengue virus (denv) is a member of the flaviviridae family, transmitted to human via mosquito. denv infection is common in tropical areas and occasionally causes life-threatening symptoms. denv contains a relatively short positive-stranded rna genome, which encodes ten viral proteins. thus, the viral life cycle is necessarily rely on or regulated by host factors. | 2016 | 26931565 |
| characterization of a novel insect-specific flavivirus from brazil: potential for inhibition of infection of arthropod cells with medically important flaviviruses. | in the past decade, there has been an upsurge in the number of newly described insect-specific flaviviruses isolated pan-globally. we recently described the isolation of a novel flavivirus (tentatively designated 'nhumirim virus'; nhuv) that represents an example of a unique subset of apparently insect-specific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses despite appearing to be limited to replication in mosquito cells. we characterized the in vitro growth p ... | 2014 | 25146007 |
| epidemiology of tick-borne encephalitis (tbe) in europe and its prevention by available vaccines. | tick-borne encephalitis (tbe), which is caused by a flavivirus, is the most common tick-transmitted disease in central and eastern europe and russia. today, tbe is endemic in 27 european countries, and has become an international public health problem. the epidemiology of tbe is changing owing to various factors, such as improvements in diagnosis and case reporting, increased recreational activities in areas populated by ticks, and changes in climatic conditions affecting tick habitats. vaccinat ... | 2013 | 23377671 |