| distribution and phylogenetic comparisons of a novel mosquito flavivirus sequence present in culex tarsalis mosquitoes from western canada with viruses isolated in california and colorado. | in a previous study, a new flavivirus genome sequence was identified in culex tarsalis mosquitoes obtained in alberta, canada and was shown to be genetically related to but distinct from members of the insect-specific flaviviruses. nonstructural protein 5-encoding sequences amplified from cx. tarsalis pools from western canada have shown a high similarity to genome sequences of novel flaviviruses isolated from mosquitoes in california and colorado. despite wide distribution of this virus, design ... | 2011 | 21734143 |
| Tembusu virus in ducks, china. | In China in 2010, a disease outbreak in egg-laying ducks was associated with a flavivirus. The virus was isolated and partially sequenced. The isolate exhibited 87%-91% identity with strains of Tembusu virus, a mosquito-borne flavivirus of the Ntaya virus group. These findings demonstrate emergence of Tembusu virus in ducks. | 2011 | 22000358 |
| Molecular evolution of the insect-specific flaviviruses. | There has been an explosion in the discovery of 'insect-specific' flaviviruses and/or their related sequences in natural mosquito populations. Herein we review all 'insect-specific' flaviviral sequences currently available and conduct phylogenetic analyses of both the 'insect-specific' flaviviruses and available sequences of the entire genus Flavivirus. We show that there is no statistical support for virus-mosquito co-divergence, suggesting that the 'insect-specific' flaviviruses may have under ... | 2011 | 22012464 |
| insect-specific flaviviruses: a systematic review of their discovery, host range, mode of transmission, superinfection exclusion potential and genomic organization. | there has been a dramatic increase in the number of insect-specific flaviviruses (isfs) discovered in the last decade. historically, these viruses have generated limited interest due to their inability to infect vertebrate cells. this viewpoint has changed in recent years because some isfs have been shown to enhance or suppress the replication of medically important flaviviruses in co-infected mosquito cells. additionally, comparative studies between isfs and medically important flaviviruses can ... | 2015 | 25866904 |
| bat airway epithelial cells: a novel tool for the study of zoonotic viruses. | bats have been increasingly recognized as reservoir of important zoonotic viruses. however, until now many attempts to isolate bat-borne viruses in cell culture have been unsuccessful. further, experimental studies on reservoir host species have been limited by the difficulty of rearing these species. the epithelium of the respiratory tract plays a central role during airborne transmission, as it is the first tissue encountered by viral particles. although several cell lines from bats were estab ... | 2014 | 24454736 |
| biological transmission of arboviruses: reexamination of and new insights into components, mechanisms, and unique traits as well as their evolutionary trends. | among animal viruses, arboviruses are unique in that they depend on arthropod vectors for transmission. field research and laboratory investigations related to the three components of this unique mode of transmission, virus, vector, and vertebrate host, have produced an enormous amount of valuable information that may be found in numerous publications. however, despite many reviews on specific viruses, diseases, or interests, a systematic approach to organizing the available information on all f ... | 0 | 16223950 |
| noncoding subgenomic flavivirus rna: multiple functions in west nile virus pathogenesis and modulation of host responses. | flaviviruses are a large group of positive strand rna viruses transmitted by arthropods that include many human pathogens such as west nile virus (wnv), japanese encephalitis virus (jev), yellow fever virus, dengue virus, and tick-borne encephalitis virus. all members in this genus tested so far are shown to produce a unique subgenomic flavivirus rna (sfrna) derived from the 3' untranslated region (utr). sfrna is a product of incomplete degradation of genomic rna by the cell 5'-3' exoribonucleas ... | 2014 | 24473339 |
| yellow fever virus capsid protein is a potent suppressor of rna silencing that binds double-stranded rna. | mosquito-borne flaviviruses, including yellow fever virus (yfv), zika virus (zikv), and west nile virus (wnv), profoundly affect human health. the successful transmission of these viruses to a human host depends on the pathogen's ability to overcome a potentially sterilizing immune response in the vector mosquito. similar to other invertebrate animals and plants, the mosquito's rna silencing pathway comprises its primary antiviral defense. although a diverse range of plant and insect viruses has ... | 2016 | 27849599 |
| a glance at subgenomic flavivirus rnas and micrornas in flavivirus infections. | the family flaviviridae comprises a wide variety of viruses that are distributed worldwide, some of which are associated with high rates of morbidity and mortality. there are neither vaccines nor antivirals for most flavivirus infections, reinforcing the importance of research on different aspects of the viral life cycle. during infection, cytoplasmic accumulation of rna fragments mainly originating from the 3' utrs, which have been designated subgenomic flavivirus rnas (sfrnas), has been detect ... | 2016 | 27233361 |
| ecuador paraiso escondido virus, a new flavivirus isolated from new world sand flies in ecuador, is the first representative of a novel clade in the genus flavivirus. | a new flavivirus, ecuador paraiso escondido virus (epev), named after the village where it was discovered, was isolated from sand flies (psathyromyia abonnenci, formerly lutzomyia abonnenci) that are unique to the new world. this represents the first sand fly-borne flavivirus identified in the new world. epev exhibited a typical flavivirus genome organization. nevertheless, the maximum pairwise amino acid sequence identity with currently recognized flaviviruses was 52.8%. phylogenetic analysis o ... | 2015 | 26355096 |
| new insights into flavivirus evolution, taxonomy and biogeographic history, extended by analysis of canonical and alternative coding sequences. | to generate the most diverse phylogenetic dataset for the flaviviruses to date, we determined the genomic sequences and phylogenetic relationships of 14 flaviviruses, of which 10 are primarily associated with culex spp. mosquitoes. we analyze these data, in conjunction with a comprehensive collection of flavivirus genomes, to characterize flavivirus evolutionary and biogeographic history in unprecedented detail and breadth. based on the presumed introduction of yellow fever virus into the americ ... | 2015 | 25719412 |
| novel flaviviruses from mosquitoes: mosquito-specific evolutionary lineages within the phylogenetic group of mosquito-borne flaviviruses. | novel flaviviruses that are genetically related to pathogenic mosquito-borne flaviviruses (mbfv) have been isolated from mosquitoes in various geographical locations, including finland. we isolated and characterized another novel virus of this group from finnish mosquitoes collected in 2007, designated as ilomantsi virus (ilov). unlike the mbfv that infect both vertebrates and mosquitoes, the mbfv-related viruses appear to be specific to mosquitoes similar to the insect-specific flaviviruses (is ... | 2014 | 25108382 |
| identification of a flavivirus sequence in a marine arthropod. | phylogenetic analysis has yet to uncover the early origins of flaviviruses. in this study, i mined a database of expressed sequence tags in order to discover novel flavivirus sequences. flavivirus sequences were identified in a pool of mrna extracted from the sea spider endeis spinosa (pycnogonida, pantopoda). reconstruction of the translated sequences and blast analysis matched the sequence to the flavivirus ns5 gene. additional sequences corresponding to envelope and the ns5 mtase domain were ... | 2015 | 26717191 |
| evaluation of a field-portable dna microarray platform and nucleic acid amplification strategies for the detection of arboviruses, arthropods, and bloodmeals. | highly multiplexed assays, such as microarrays, can benefit arbovirus surveillance by allowing researchers to screen for hundreds of targets at once. we evaluated amplification strategies and the practicality of a portable dna microarray platform to analyze virus-infected mosquitoes. the prototype microarray design used here targeted the non-structural protein 5, ribosomal rna, and cytochrome b genes for the detection of flaviviruses, mosquitoes, and bloodmeals, respectively. we identified 13 of ... | 2012 | 23249687 |
| rapid evolutionary dynamics of structural disorder as a potential driving force for biological divergence in flaviviruses. | protein structure is commonly regarded to be conserved and to dictate function. most proteins rely on conformational flexibility to some degree. are regions that convey conformational flexibility conserved over evolutionary time? can changes in conformational flexibility alter protein function? here, the evolutionary dynamics of structurally ordered and disordered (flexible) regions are investigated genome-wide in flaviviruses, revealing that the amount and location of structural disorder fluctu ... | 0 | 23418179 |
| novel insect-specific flavivirus isolated from northern europe. | mosquitoes collected in finland were screened for flaviviral rna leading to the discovery and isolation of a novel flavivirus designated hanko virus (hankv). virus characterization, including phylogenetic analysis of the complete coding sequence, confirmed hankv as a member of the "insect-specific" flavivirus (isf) group. hankv is the first member of this group isolated from northern europe, and therefore the first northern european isf for which the complete coding sequence has been determined. ... | 2012 | 22999256 |
| zika virus on a spreading spree: what we now know that was unknown in the 1950's. | zika virus (zikv) is a mosquito-borne flavivirus that is transmitted through the bite of aedes spp mosquitoes and less predominantly, through sexual intercourse. prior to 2007, zikv was associated with only sporadic human infections with minimal or no clinical manifestations. recently the virus has caused disease outbreaks from the pacific islands, the americas, and off the coast of west africa with approximately 1.62 million people suspected to be infected in more than 60 countries around the g ... | 2016 | 27716242 |
| pathogenesis of modoc virus (flaviviridae; flavivirus) in persistently infected hamsters. | the long-term persistence of modoc virus (modv) infection was investigated in a hamster model. golden hamsters (mesocricetus auratus) were infected by subcutaneous inoculation with modv, in which fatal encephalitis developed in 12.5% (2 of 16). surviving hamsters shed infectious modv in their urine during the first five months after infection, and infectious modv was recovered by co-cultivation of kidney tissue up to eight months after infection. there were no histopathologic changes observed in ... | 2013 | 23358636 |
| substitution of the premembrane and envelope protein genes of modoc virus with the homologous sequences of west nile virus generates a chimeric virus that replicates in vertebrate but not mosquito cells. | most known flaviviruses, including west nile virus (wnv), are maintained in natural transmission cycles between hematophagous arthropods and vertebrate hosts. other flaviviruses such as modoc virus (modv) and culex flavivirus (cxfv) have host ranges restricted to vertebrates and insects, respectively. the genetic elements that modulate the differential host ranges and transmission cycles of these viruses have not been identified. | 2014 | 25151534 |
| characterization of a novel insect-specific flavivirus from brazil: potential for inhibition of infection of arthropod cells with medically important flaviviruses. | in the past decade, there has been an upsurge in the number of newly described insect-specific flaviviruses isolated pan-globally. we recently described the isolation of a novel flavivirus (tentatively designated 'nhumirim virus'; nhuv) that represents an example of a unique subset of apparently insect-specific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses despite appearing to be limited to replication in mosquito cells. we characterized the in vitro growth p ... | 2014 | 25146007 |
| serological evidence of flaviviruses and alphaviruses in livestock and wildlife in trinidad. | seroprevalence rates of selected arboviruses in animal populations in trinidad were determined using serum samples collected between 2006 and 2009 from horses (n=506), cattle (n=163), sheep (n=198), goats (n=82), pigs (n=184), birds (n=140), rodents (n=116), and other vertebrates (n=23). the sera were screened for antibodies to west nile virus (wnv), st. louis encephalitis virus (slev), ilheus virus (ilhv), bussuquara virus (bsqv), venezuelan equine encephalitis virus (veev), eastern equine ence ... | 2012 | 22989182 |
| structural and functional analyses of a conserved hydrophobic pocket of flavivirus methyltransferase. | the flavivirus methyltransferase (mtase) sequentially methylates the n7 and 2'-o positions of the viral rna cap (gpppa-rna → m(7)gpppa-rna → m(7)gpppam-rna), using s-adenosyl-l-methionine (adomet) as a methyl donor. we report here that sinefungin (sin), an adomet analog, inhibits several flaviviruses through suppression of viral mtase. the crystal structure of west nile virus mtase in complex with sin inhibitor at 2.0-å resolution revealed a flavivirus-conserved hydrophobic pocket located next t ... | 2010 | 20685660 |
| structure and functionality in flavivirus ns-proteins: perspectives for drug design. | flaviviridae are small enveloped viruses hosting a positive-sense single-stranded rna genome. besides yellow fever virus, a landmark case in the history of virology, members of the flavivirus genus, such as west nile virus and dengue virus, are increasingly gaining attention due to their re-emergence and incidence in different areas of the world. additional environmental and demographic considerations suggest that novel or known flaviviruses will continue to emerge in the future. nevertheless, u ... | 2010 | 19945487 |
| characterization of a novel flavivirus from mosquitoes in northern europe that is related to mosquito-borne flaviviruses of the tropics. | a novel flavivirus was isolated from mosquitoes in finland, representing the first mosquito-borne flavivirus from northern europe. the isolate, designated lammi virus (lamv), was antigenically cross-reactive with other flaviviruses and exhibited typical flavivirus morphology as determined by electron microscopy. the genomic sequence of lamv was highly divergent from the recognized flaviviruses, and yet the polyprotein properties resembled those of mosquito-borne flaviviruses. phylogenetic analys ... | 2009 | 19570865 |
| structural bases for substrate recognition and activity in meaban virus nucleoside-2'-o-methyltransferase. | viral methyltransferases are involved in the mrna capping process, resulting in the transfer of a methyl group from s-adenosyl-l-methionine to capped rna. two groups of methyltransferases (mtases) are known: (guanine-n7)-methyltransferases (n7mtases), adding a methyl group onto the n7 atom of guanine, and (nucleoside-2'-o-)-methyltransferases (2'omtases), adding a methyl group to a ribose hydroxyl. we have expressed and purified two constructs of meaban virus (mv; genus flavivirus) ns5 protein m ... | 2007 | 17473012 |
| bats: important reservoir hosts of emerging viruses. | bats (order chiroptera, suborders megachiroptera ["flying foxes"] and microchiroptera) are abundant, diverse, and geographically widespread. these mammals provide us with resources, but their importance is minimized and many of their populations and species are at risk, even threatened or endangered. some of their characteristics (food choices, colonial or solitary nature, population structure, ability to fly, seasonal migration and daily movement patterns, torpor and hibernation, life span, roo ... | 2006 | 16847084 |
| association between hepatitis c virus and very-low-density lipoprotein (vldl)/ldl analyzed in iodixanol density gradients. | hepatitis c virus (hcv) rna circulates in the blood of persistently infected patients in lipoviroparticles (lvps), which are heterogeneous in density and associated with host lipoproteins and antibodies. the variability and lability of these virus-host complexes on fractionation has hindered our understanding of the structure of lvp and determination of the physicochemical properties of the hcv virion. in this study, hcv from an antibody-negative immunodeficient patient was analyzed using three ... | 2006 | 16474148 |
| the modeled structure of the rna dependent rna polymerase of gbv-c virus suggests a role for motif e in flaviviridae rna polymerases. | the flaviviridae virus family includes major human and animal pathogens. the rna dependent rna polymerase (rdrp) plays a central role in the replication process, and thus is a validated target for antiviral drugs. despite the increasing structural and enzymatic characterization of viral rdrps, detailed molecular replication mechanisms remain unclear. the hepatitis c virus (hcv) is a major human pathogen difficult to study in cultured cells. the bovine viral diarrhea virus (bvdv) is often used as ... | 2005 | 16225688 |
| low diversity of alkhurma hemorrhagic fever virus, saudi arabia, 1994-1999. | alkhurma hemorrhagic fever virus (genus flavivirus, ahfv) was recently identified as the agent of a viral hemorrhagic fever in saudi arabia and characterized serologically and genetically as a variant genotype of kyasanur forest disease virus (kfdv). since viral diagnosis and vaccine development may be hindered by genetic diversity, this study was intended to address ahfv genetic heterogeneity. eleven strains isolated from hospitalized patients from 1994 to 1999 in saudi arabia were sequenced in ... | 2005 | 15890119 |
| genetic characterization of yokose virus, a flavivirus isolated from the bat in japan. | yokose virus (strain oita-36) was isolated from the bat in japan in 1971. in the present study, we determined complete nucleotide sequences of yokose virus using rt-pcr and race techniques. yokose virus genome consists of 10,857 nucleotides in length (accession no. ab114858), containing a single open reading frame (3425 amino acids) encoding 11 viral proteins. we deduced the boundaries of each protein in the polyprotein sequence according to the protein cleavage sites of other flaviviruses. the ... | 2005 | 15661139 |
| latent infection of rio bravo virus in salivary glands of bats. | | 1964 | 14234343 |
| universal primers that amplify rna from all three flavivirus subgroups. | species within the flavivirus genus pose public health problems around the world. increasing cases of dengue and japanese encephalitis virus in asia, frequent outbreaks of yellow fever virus in africa and south america, and the ongoing spread of west nile virus throughout the americas, show the geographical burden of flavivirus diseases. flavivirus infections are often indistinct from and confused with other febrile illnesses. here we review the specificity of published primers, and describe a n ... | 2008 | 18218114 |
| structure of yellow fever virus envelope protein domain iii. | the structure of recombinant domain iii of the envelope protein (red3) of yellow fever virus (yfv), containing the major neutralization site, was determined using nmr spectroscopy. the amino acid sequence and structure of the yfv-red3 shows differences from ed3s of other mosquito-borne flaviviruses; in particular, the partially surface-exposed bc loop where methionine-304 and valine-324 were identified as being critical for the structure of the loop. variations in the structure and surface chemi ... | 2009 | 19818466 |
| growth of rio bravo virus in cell cultures. | the growth of rio bravo virus (rbv) in eight cell culture systems was studied. highest yields of virus were produced in bhk-21 (c13), l, and vero cell lines, but l cells were resistant to low doses of virus. llc-mk(2), hela, and human embryo skin cells produced moderate amounts of virus, but fl amnion and primary chick embryo fibroblasts supported little virus growth. virus was rapidly inactivated by exposure to ph values below 7.0. single-cycle growth in bhk-21, l, and llc-mk(2) cell monolayers ... | 1970 | 5498612 |
| characterization of a siberian virus isolated from a patient with progressive chronic tick-borne encephalitis. | a strain of tick-borne encephalitis virus designated zausaev (za) was isolated in siberia from a patient who died of a progressive (2-year) form of tick-borne encephalitis 10 years after being bitten by a tick. the complete genomic sequence of this virus was determined, and an attempt was made to correlate the sequence with the biological characteristics of the virus. phylogenetic analysis demonstrated that this virus belongs to the siberian subtype of tick-borne encephalitis virus. comparison o ... | 2003 | 12477807 |
| exchanging the yellow fever virus envelope proteins with modoc virus prm and e proteins results in a chimeric virus that is neuroinvasive in scid mice. | a chimeric flavivirus infectious cdna was constructed by exchanging the premembrane (prm) and envelope (e) genes of the yellow fever virus vaccine strain 17d (yf17d) with the corresponding genes of modoc virus (mod). this latter virus belongs to the cluster of the "not-known vector" flaviviruses and is, unlike yf17d, neuroinvasive in scid mice. replication of in vitro-transcribed rna from this chimeric flavivirus was shown by [(3)h]uridine labeling and rna analysis. expression of the mod prm and ... | 2004 | 15220415 |
| viral rna switch mediates the dynamic control of flavivirus replicase recruitment by genome cyclization. | viral replicase recruitment and long-range rna interactions are essential for rna virus replication, yet the mechanism of their interplay remains elusive. flaviviruses include numerous important human pathogens, e.g., dengue virus (denv) and zika virus (zikv). here, we revealed a highly conserved, conformation-tunable cis-acting element named 5'-uar-flanking stem (ufs) in the flavivirus genomic 5' terminus. we demonstrated that the ufs was critical for efficient ns5 recruitment and viral rna syn ... | 2016 | 27692070 |
| flaviviruses in europe: complex circulation patterns and their consequences for the diagnosis and control of west nile disease. | in europe, many flaviviruses are endemic (west nile, usutu, tick-borne encephalitis viruses) or occasionally imported (dengue, yellow fever viruses). due to the temporal and geographical co-circulation of flaviviruses in europe, flavivirus differentiation by diagnostic tests is crucial in the adaptation of surveillance and control efforts. serological diagnosis of flavivirus infections is complicated by the antigenic similarities among the flavivirus genus. indeed, most flavivirus antibodies are ... | 2013 | 24225644 |
| ilheus virus isolation in the pantanal, west-central brazil. | the wetlands of the brazilian pantanal host large concentrations of diverse wildlife species and hematophagous arthropods, conditions that favor the circulation of zoonotic arboviruses. a recent study from the nhecolândia sub-region of pantanal reported serological evidence of various flaviviruses, including west nile virus and ilheus virus (ilhv). according to the age of seropositive horses, at least three flaviviruses, including ilhv, circulated in the brazilian pantanal between 2005 and 2009. ... | 2013 | 23875051 |
| flavidb: a data mining system for knowledge discovery in flaviviruses with direct applications in immunology and vaccinology. | the flavivirus genus is unusually large, comprising more than 70 species, of which more than half are known human pathogens. it includes a set of clinically relevant infectious agents such as dengue, west nile, yellow fever, and japanese encephalitis viruses. although these pathogens have been studied extensively, safe and efficient vaccines lack for the majority of the flaviviruses. | 0 | 25544857 |
| serologic evidence of flavivirus infection in bats in the yucatan peninsula of mexico. | we captured 140 bats of seven species in merida city in the yucatan peninsula of mexico in 2010. serum was collected from each bat and assayed by plaque reduction neutralization test (prnt) using six flaviviruses: west nile virus, st. louis encephalitis virus, and dengue viruses 1-4. flavivirus-specific antibodies were detected in 26 bats (19%). the antibody-positive bats belonged to three species: the pallas's long-tongued bat (glossophaga soricina), jamaican fruit bat (artibeus jamaicensis), a ... | 0 | 23778622 |
| genetic characterization of an insect-specific flavivirus isolated from culex theileri mosquitoes collected in southern portugal. | we describe the full genetic characterization of an insect-specific flavivirus (isf) from culex theileri (theobald) mosquitoes collected in portugal. this represents the first isolation and full characterization of an isf from portuguese mosquitoes. the virus, designated ctfv, for culex theileri flavivirus, was isolated in the c6/36 stegomyia albopicta (=aedes albopictus) cell line, and failed to replicate in vertebrate (vero) cells in common with other isfs. the ctfv genome encodes a single pol ... | 2012 | 22579596 |
| detection of entebbe bat virus after 54 years. | entebbe bat virus (entv; flaviviridae: flavivirus), closely related to yellow fever virus, was first isolated from a little free-tailed bat (chaerephon pumilus) in uganda in 1957, but was not detected after that initial isolation. in 2011, we isolated entv from a little free-tailed bat captured from the attic of a house near where it had originally been found. infectious virus was recovered from the spleen and lung, and the viral rna was sequenced and compared with that of the original isolate. ... | 2015 | 26101270 |
| characterisation of divergent flavivirus ns3 and ns5 protein sequences detected in rhipicephalus microplus ticks from brazil. | transcripts similar to those that encode the nonstructural (ns) proteins ns3 and ns5 from flaviviruses were found in a salivary gland (sg) complementary dna (cdna) library from the cattle tick rhipicephalus microplus. tick extracts were cultured with cells to enable the isolation of viruses capable of replicating in cultured invertebrate and vertebrate cells. deep sequencing of the viral rna isolated from culture supernatants provided the complete coding sequences for the ns3 and ns5 proteins an ... | 2013 | 24626302 |
| transmission or within-host dynamics driving pulses of zoonotic viruses in reservoir-host populations. | progress in combatting zoonoses that emerge from wildlife is often constrained by limited knowledge of the biology of pathogens within reservoir hosts. we focus on the host-pathogen dynamics of four emerging viruses associated with bats: hendra, nipah, ebola, and marburg viruses. spillover of bat infections to humans and domestic animals often coincides with pulses of viral excretion within bat populations, but the mechanisms driving such pulses are unclear. three hypotheses dominate current res ... | 2016 | 27489944 |
| full genomic sequence of the prototype strain (m64) of rio bravo virus. | rio bravo virus (rbv) is a member of the family flaviviridae, genus flavivirus. it belongs to a group of viruses in the genus with no known vector. in this report, we analyze the complete genome of the prototype rbv, strain m64. | 0 | 22457530 |
| biochemical and biophysical characteristics of rio bravo virus (flaviviridae). | rio bravo (rb) virus has been assigned to the family flaviviridae on the basis of its antigenic relatedness to other members of this family. rb virus, unlike most members of the flaviviridae, is believed not to have an arthropod vector. we examined biochemical and biophysical characteristics of rb virus to determine whether it should be assigned to the flaviviridae and to compare it with arthropod-borne flaviviruses. purified rb virus banded at a density of 1.18 g/ml in sucrose and had a sedimen ... | 1988 | 2828514 |
| seroepidemiology of selected alphaviruses and flaviviruses in bats in trinidad. | a serosurvey of antibodies against selected flaviviruses and alphaviruses in 384 bats (representing 10 genera and 14 species) was conducted in the caribbean island of trinidad. sera were analysed using epitope-blocking enzyme-linked immunosorbent assays (elisas) specific for antibodies against west nile virus (wnv), venezuelan equine encephalitis virus (veev) and eastern equine encephalitis virus (eeev), all of which are zoonotic viruses of public health significance in the region. overall, the ... | 2015 | 24751420 |
| biophysical characterization and vector-specific antagonist activity of domain iii of the tick-borne flavivirus envelope protein. | the molecular determinants responsible for flavivirus host cell binding and tissue tropism are largely unknown, although domain iii of the envelope protein has been implicated in these functions. we examined the solution properties and antagonist activity of langat virus domain iii. our results suggest that domain iii adopts a stably folded structure that can mediate binding of tick-borne flaviviruses but not mosquito-borne flaviviruses to their target cells. three clusters of phylogenetically c ... | 2001 | 11264392 |
| [chiroptera viruses transmitted or not transmitted by arthropods]. | the authors make a review of literature on viruses isolated from chiroptera's tissues. they study especially two of these viruses: a true arbovirus, the japanese b encephalitis virus and a virus of the same genus but not arthropod-borne, the rio bravo virus. they consider successively for each virus: isolations from bats, serosurveys in bat and in man, and experimental studies with these viruses, allowing to estimate the role of chiroptera in the diffusion and the maintenance of some arboviruses ... | 1986 | 3807758 |
| detection of quang binh virus from mosquitoes in china. | flaviviruses present a wide range of genetic diversity and exhibit diverse host relationships. mosquito-borne flaviviruses have recently been isolated and characterized worldwide. yunnan province of china is one of the richest areas of species diversity and is the center of multi-species evolution in mainland asia, which supports the circulation of numerous arthropod-borne viruses (arboviruses). in a screening program of arboviruses, mosquitoes were collected during the mosquito activity season ... | 2014 | 24342141 |
| serological evidence of widespread exposure of grenada fruit bats to chikungunya virus. | antibody detection against selected potentially zoonotic vector-borne alphaviruses and flaviviruses was conducted on sera from bats from all six parishes in grenada, west indies. sera were tested for (i) antibodies to flaviviruses west nile virus, st. louis encephalitis virus, ilhéus virus, bussuquara virus (bsqv), rio bravo virus and all four serotypes of dengue virus (denv) by plaque reduction neutralization test (prnt); (ii) antibodies to alphaviruses western equine encephalitis virus, venezu ... | 2018 | 29575672 |