| real-time pcr identification of unique bacillus anthracis sequences. | bacillus anthracis is a spore-forming, gram-positive microorganism. it is a causative agent of anthrax, a highly infectious disease. it belongs to the "bacillus cereus group", which contains other closely related species, including bacillus cereus, bacillus thuringiensis, bacillus mycoides, bacillus weihenstephanensis, and bacillus pseudomycoides. b. anthracis naturally occurs in soil environments. the ba5345 genetic marker was used for highly specific detection of b. anthracis with taqman probe ... | 2015 | 26667574 |
| bacteria present in comadia redtenbacheri larvae (lepidoptera: cossidae). | the external and internal culturable bacterial community present in the larvae of comadia redtenbacheri hammerschmidt, an edible insect, was studied. characterization of the isolates determined the existence of 18 morphotypes and phylogenetic analysis of the 16s rrna gene revealed the existence of paenibacillus sp., bacillus safensis, pseudomonas sp., bacillus pseudomycoides, corynebacterium variabile, enterococcus sp., gordonia sp., acinetobacter calcoaceticus, arthrobacter sp., micrococcus sp. ... | 2015 | 26336239 |
| bacillus manliponensis sp. nov., a new member of the bacillus cereus group isolated from foreshore tidal flat sediment. | a gram-positive, endospore-forming, new bacillus species, strain bl4-6(t), was isolated from tidal flat sediment of the yellow sea. strain bl4-6(t) is a straight rod, with motility by peritrichate flagella. the cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. the major fatty acids are iso-c(15:0) and summed feature 3 (containing c(16:1) ω7c/iso-c(15:0) 2oh, and/or iso-c(15:0) 2oh/c(16:1) ω7c). cells are catalase-positive and oxidase-negative. the g ... | 2011 | 22203569 |
| mutually exclusive distribution of the sap and eag s-layer genes and the lytb/lyta cell wall hydrolase genes in bacillus thuringiensis. | recently, two bacillus thuringiensis strains were reported to synthesize parasporal inclusion bodies made not of the expected crystal (cry) proteins but rather of the surface layer proteins (slp) sap (encoded by sap) and ea1 (encoded by eag), respectively. whether the presence of the sap and eag genes is restricted to these two b. thuringiensis strains or ubiquitous in b. thuringiensis is unknown. we report here the distribution of the sap and eag genes in b. thuringiensis. strains in the bacill ... | 2011 | 21611767 |
| bacillus gaemokensis sp. nov., isolated from foreshore tidal flat sediment from the yellow sea. | a gram-positive, rod-shaped, endospore-forming organism, strain bl3-6(t), was isolated from tidal flat sediments of the yellow sea in the region of tae-an. a 16s rrna gene sequence analysis demonstrated that this isolate belongs to the bacillus cereus group, and is closely related to bacillus mycoides (99.0% similarity), bacillus thuringiensis (99.0%), bacillus weihenstephanensis (99.0%), bacillus cereus (98.9%), bacillus anthracis (98.8%), and bacillus pseudomycoides (98.1%). the phylogenetic d ... | 2010 | 21221948 |
| development and validation of a real-time quantitative pcr assay for rapid identification of bacillus anthracis in environmental samples. | a real-time polymerase chain reaction (pcr) assay was developed for rapid identification of bacillus anthracis in environmental samples. these samples often harbor bacillus cereus bacteria closely related to b. anthracis, which may hinder its specific identification by resulting in false positive signals. the assay consists of two duplex real-time pcr: the first pcr allows amplification of a sequence specific of the b. cereus group (b. anthracis, b. cereus, bacillus thuringiensis, bacillus weihe ... | 2010 | 20827474 |
| [identification of a fibrinolytic enzyme producing bacillus pseudomycoides and purification and characterization of the enzyme]. | to purify a single fibrinolytic enzyme from bacillus pseudomycoides b-60 and to determine its n-terminal sequence and to characterize the fibrinolytic enzyme. | 2009 | 19621637 |
| glycosyltransferase: a specific marker for the discrimination of bacillus anthracis from the bacillus cereus group. | bacillus anthracis, the aetiological agent of anthrax, has been taxonomically classified with the bacillus cereus group, which comprises b. cereus, bacillus thuringiensis, bacillus mycoides, bacillus pseudomycoides and bacillus weihenstephanensis. although the pathogenesis and ecological manifestations may be different, b. anthracis shares a high degree of dna sequence similarity with its group member species. as a result, the discrimination of b. anthracis from its close relatives in the b. cer ... | 2008 | 18287289 |
| susceptibility of bacillus anthracis, bacillus cereus, bacillus mycoides, bacillus pseudomycoides and bacillus thuringiensis to 24 antimicrobials using sensititre automated microbroth dilution and etest agar gradient diffusion methods. | to examine susceptibilities of bacillus anthracis and related species to 24 antimicrobials using and concurrently comparing two methods. | 2007 | 17586563 |
| occurrence and pathogenic potential of bacillus cereus group bacteria in a sandy loam. | the major part (94%) of the bacillus cereus-like isolates from a danish sandy loam are psychrotolerant bacillus weihenstephanensis according to their ability to grow at temperatures below 7 degrees c and/or two pcr-based methods, while the remaining 6% are b. cereus. the bacillus mycoides-like isolates could also be divided into psychrotolerant and mesophilic isolates. the psychrotolerant isolates of b. mycoides could be discriminated from the mesophilic by the two pcr-based methods used to char ... | 2006 | 16710635 |
| diversity of commensal bacillus cereus sensu lato isolated from the common sow bug (porcellio scaber, isopoda). | although bacillus cereus sensu lato are important both from an ecological and an economical point of view, little is known about their population structure, ecology, and relationships with other organisms. in the present work, the genotypic similarity of arthropod-borne b. cereus s.l. isolates, and their symbiotic relationship with the host are assessed. bacilli of this group were recovered from the digestive tracts of sow bugs (porcellio scaber) collected in three closely located sites. their g ... | 2006 | 16542411 |
| the autolytic phenotype of the bacillus cereus group. | to determine the autolytic phenotype of five species in the bacillus cereus group. | 2005 | 16238737 |
| homoduplex and heteroduplex polymorphisms of the amplified ribosomal 16s-23s internal transcribed spacers describe genetic relationships in the "bacillus cereus group". | bacillus anthracis, bacillus cereus, bacillus mycoides, bacillus pseudomycoides, bacillus thuringiensis, and bacillus weihenstephanensis are closely related in phenotype and genotype, and their genetic relationship is still open to debate. the present work uses amplified 16s-23s internal transcribed spacers (its) to discriminate between the strains and species and to describe the genetic relationships within the "b. cereus group," advantage being taken of homoduplex-heteroduplex polymorphisms (h ... | 2000 | 11097928 |
| the hemolytic enterotoxin hbl is broadly distributed among species of the bacillus cereus group. | the prevalence of the hemolytic enterotoxin complex hbl was determined in all species of the bacillus cereus group with the exception of bacillus anthracis. hbla, encoding the binding subunit b, was detected by pcr and southern analysis and was confirmed by partial sequencing of 18 strains. the sequences formed two clusters, one including b. cereus and bacillus thuringiensis strains and the other one consisting of bacillus mycoides, bacillus pseudomycoides, and bacillus weihenstephanensis strain ... | 1999 | 10584001 |
| bacillus pseudomycoides sp. nov. | previous dna relatedness studies showed that strains identified as bacillus mycoides segregated into two genetically distinct yet phenotypically similar groups, one being b. mycoides sensu stricto and the other, an unclassified taxon. in the present study, the taxonomic position of this second group was assessed by measuring dna relatedness and determining phenotypic characteristics of an increased number of b. mycoides strains. also determined was the second group's 16s rna gene sequence. the 3 ... | 1998 | 9734060 |
| bacillus bingmayongensis sp. nov., isolated from the pit soil of emperor qin's terra-cotta warriors in china. | a bacillus-like isolate, strain fjat-13831(t), isolated from the no. 1 pit soil of emperor qin's terra-cotta warriors in xi'an city, china, was studied to determine its taxonomic status. dominant fatty acids of this organism included iso-c15:0, iso-c17:0, c16:0, iso-c13:0, anteiso-c15:0, and iso-c17:1ω5c. comparative 16s rrna gene sequence analysis confirmed the affiliation of this isolate to the genus bacillus and indicated that it was closely related to bacillus pseudomycoides dsm 12442(t) (99 ... | 2014 | 24370979 |
| pseudomycoicidin, a class ii lantibiotic from bacillus pseudomycoides. | lantibiotics are ribosomally synthesized antimicrobial peptides with substantial posttranslational modifications. they are characterized by the unique amino acids lanthionine and methyllanthionine, which are introduced by dehydration of ser/thr residues and linkage of the resulting dehydrated amino acids with cys residues. blast searches using the mersacidin biosynthetic enzyme (mrsm) in the ncbi database revealed a new class ii lantibiotic gene cluster in bacillus pseudomycoides dsm 12442. prod ... | 2015 | 25769830 |
| assessment of culturable tea rhizobacteria isolated from tea estates of assam, india for growth promotion in commercial tea cultivars. | in the present study, 217 rhizobacterial isolates were obtained from six different tea estates of assam, india and subjected to preliminary in vitro plant growth promotion (pgp) screening for indole acetic acid (iaa) production, phosphate solubilization, siderophore production and ammonia production. fifty isolates showed all the pgp traits and five isolates did not exhibit any pgp traits. these 50 potential isolates were further analyzed for quantitative estimation of the pgp traits along with ... | 2015 | 26617590 |
| analysis of comparative sequence and genomic data to verify phylogenetic relationship and explore a new subfamily of bacterial lipases. | thermostable and organic solvent-tolerant enzymes have significant potential in a wide range of synthetic reactions in industry due to their inherent stability at high temperatures and their ability to endure harsh organic solvents. in this study, a novel gene encoding a true lipase was isolated by construction of a genomic dna library of thermophilic aneurinibacillus thermoaerophilus strain hz into escherichia coli plasmid vector. sequence analysis revealed that hz lipase had 62% identity to pu ... | 2016 | 26934700 |
| family portrait of bacillus cereus and bacillus weihenstephanensis cereulide-producing strains. | two thousand bacillus cereus sensu lato isolates from food and environmental matrices were screened by pcr for the presence of cereulide-producing strains. this survey identified 73 potential emetic strains, most of which originated from non-random food and clinical samplings. none of the 460 bacillus thuringiensis, bacillus mycoides and bacillus pseudomycoides strains were pcr-positive for the cereulide genetic determinants. the chromosomal and extrachromosomal gene pool diversity of a subset o ... | 2009 | 23765791 |
| isolation and characterization of spore-forming bacilli (sfb) from shepherd's purse (capsella bursa-pastoris). | shepherd's purse (capsella bursa-pastoris), native to europe, is commonly consumed fresh and sometimes inadequately washed before consumption in korea. the objective of this study was to characterize isolates of spore-forming bacilli (sfb) in samples of fresh shepherd's purse. three genera were identified: bacillus (9 species), paenibacillus (3 species), and brevibacillus (1 species). none of the genes of the hemolysin bl (hbl) and nonhemolytic enterotoxin (nhe) complexes, or of the emetic toxin ... | 2016 | 26822957 |
| multilocus sequence analysis of bacillus thuringiensis serovars navarrensis, bolivia and vazensis and bacillus weihenstephanensis reveals a common phylogeny. | the bacillus cereus group sensu lato includes six closely-related bacterial species: bacillus cereus, bacillus anthracis, bacillus thuringiensis, bacillus mycoides, bacillus pseudomycoides and bacillus weihenstephanensis. b. thuringiensis is distinguished from the other species mainly by the appearance of an inclusion body upon sporulation. b. weihenstephanensis is distinguished based on its psychrotolerance and the presence of specific signature sequences in the 16s rrna gene and cspa genes. a ... | 2013 | 23073664 |
| bacillus weihenstephanensis characteristics are present in bacillus cereus and bacillus mycoides strains. | the bacillus cereus group comprises seven bacterial species: bacillus cereus, bacillus anthracis, bacillus thuringiensis, bacillus mycoides, bacillus pseudomycoides, bacillus cytotoxicus, and bacillus weihenstephanensis. bacillus weihenstephanensis is distinguished based on its capability to grow at 7 °c but not at 43 °c, and the presence of specific signature sequences in the 16s rrna and cspa genes and in several housekeeping genes: glpf, gmk, purh, and tpi. bacillus weihenstephanensis-specifi ... | 2013 | 23413955 |
| the members of the bacillus cereus group are commonly present contaminants of fresh and heat-treated milk. | to determine the level of milk contamination by bacillus cereus sensu lato, 44 samples were collected from a dairy farm and two independent dairies in northeastern poland. a total of 680 b. cereus sensu lato isolates were recovered. based on spore counts, their highest level in milk was found during the spring and summer months. although significant variations in chromosomal dna polymorphisms among b. cereus sensu lato isolates were noted based on repetitive element sequence polymorphism (rep-pc ... | 2008 | 18456114 |
| differentiation of strains from the bacillus cereus group by rflp-pfge genomic fingerprinting. | bacillus mycoides, bacillus pseudomycoides, bacillus weihenstephanensis, bacillus anthracis, bacillus thuringiensis, and bacillus cereus belong to the b. cereus group. the last three species are characterized by different phenotype features and pathogenicity spectrum, but it has been shown that these species are genetically closely related. the macrorestriction analysis of the genomic dna with the noti enzyme was used to generate polymorphism of restriction profiles for 39 food-borne isolates (b ... | 2013 | 23893780 |