cytokinesis in impatiens balsamina and the effect of caffeine. | a description of cytokinesis in cells of roots of impatiens balsamina is given, and the effect of caffeine on this process. the disposition of organelles, microtubules and vesicles which form the new cell plate is similar in normal and caffeine treated roots. in sections cut in the plane of the developing cell plate, membranous arms radiating from common centres appear to play an important role in promoting efficient fusion of the vesicles, and caffeine reduces their occurrence. the possible pre ... | 1978 | 743893 |
scanning electron microscopy in nematode-induced giant transfer cells. | a study of giant cells induced by the root-knot nematode, meloidogyne incognita, in roots of impatiens balsamina was made by scanning electron microscopy. the cytoplasmic contents of giant cells were removed by a procedure based on koh digestion, to reveal inner wall structure. wall ingrowths typical of transfer cells are present in giant cells from six days onwards after induction. they develop on walls adjacent to vascular tissues, and their distribution and development was examined. pit field ... | 1976 | 1001022 |
accumulation of cadmium by plants of zawar mines, rajasthan, india. | several plants of the zawar zinc deposits of rajasthan (india) accumulate very high levels of cadmium. the maximum concentration namely 420 micrograms g-1 was found in the stems of crotalaria linifolia. some other cadmium accumulators with the concentration of the metal in microgram g-1 are impatients balsamina (380), dyerophytum indicum (282) and melhania futteyporensis (245). the sequence of cadmium accumulation in different organs of the same plant species was roots greater than stems greater ... | 1988 | 3254012 |
bioindicators and accumulators in geobotanical and biogeochemical prospecting of metals. | results of geobotanical and biogeochemical studies carried out on the known lead-zinc deposits of zawar mines and khetri copper deposits, india, are discussed. a wild variety of impatiens balsamina was found to be the most characteristic species on lead-zinc metal dumps, which can be regarded as a local 'bioindicator' for these metals. a number of 'accumulators' for lead, zinc, copper with high bac values, as well as some 'excluders' are also discussed. | 1986 | 3591164 |
on the possible involvement of chloroplasts from impatiens balsamina in the biosynthesis of flavonoid compounds. | | 1972 | 5027697 |
naphthoquinone biosynthesis in higher plants. i. studies on 2-hydroxy-1,4-naphthoquinone in impatiens balsamina l. | | 1966 | 5954117 |
occurrence of two powdery mildews on balsam, impatiens balsamina l. | | 1966 | 6007588 |
naphthoquinone biosynthesis in higher plants. ii. p-hydroxybenzoic acid as a precursor of 2-hydroxy 1,4-naphthoquinone in impatiens balsamina l. | | 1967 | 6049360 |
a simple acetic-orcein banding method for precise chromosome identification and detection of constitutive heterochromatin in impatiens balsamina l. | | 1982 | 6175925 |
the role of controlling elements in the instability of flower color in antirrhinum majus and impatiens balsamina. | | 1981 | 6266742 |
baccharane glycosides from seeds of impatiens balsamina. | from the seeds of impatiens balsamina have been isolated four rare baccharane glycosides, hosenkosides l-o. the structures of all isolates were secured by the use of 2d nmr techniques (1h-1h cosy, hmqc, hmbc, roesy) and chemical derivatization. hosenkosides l and m are hosenkol a 3-o-sambubiosyl-28-o-glucoside and 3-o-sambubiosyl-26-o-glucosyl-28-o-glucoside, respectively. hosenkoside n is hosenkol c 3-o-glucosyl-28-o-glucoside. hosenkoside o is hosenkol d 3-o-sophorosyl-28-o-glucoside. | 1994 | 7765763 |
structure determination of a kaempferol 3-rhamnosyldiglucoside from impatiens balsamina. | | 1994 | 7765767 |
hosenkosides f, g, h, i, j, and k, novel baccharane glycosides from the seeds of impatiens balsamina. | from the seeds of impatiens balsamina we have isolated six novel baccharane glycosides, hosenkosides f-k. the structures of all isolates were determined by the use of two dimensional (2d) nmr techniques (1h-1h correlation spectroscopy (cosy), heteronuclear multiple quantum coherence (hmqc), heteronuclear multiple-bond correlation (hmbc), rotating frame overhauser enhancement spectroscopy (roesy), total correlation spectroscopy (tocsy)) and chemical derivatization. hosenkosides f, h and i are hos ... | 1994 | 7923465 |
potential sources of sugar for the phlebotomine sandfly lutzomyia youngi (diptera: psychodidae) in a colombian coffee plantation. | sugar-deprived lutzomyia youngi were exposed to each of the five most numerous plant species in a colombian coffee plantation for 24 h and then tested for the presence of fructose by the cold anthrone assay. many flies exposed to coffee (coffea arabica) (up to 55% in an individual test) and guamo (inga edulis) (up to 28.0%) gave positive results. although some flies also took sugar from red-flowered balsam (impatiens balsamina) and bamboo (bambusa angustifolia), none of the insects exposed to pl ... | 1994 | 7979645 |
a novel protein accumulated during maturation of the pods of the plant impatiens balsamina. | a high molecular weight protein has been isolated as a major polypeptide comprising 85% of the total extractable proteins in the fruit pericarp of the plant impatiens balsamina. the protein has been purified to homogeneity following fractionation of the crude cell supernatant with ammonium sulphate, chromatography on sepharose 4b and sephacryl-s-300. this protein appeared to be a homo-tetramer consisting of subunits, mr 75 k. amino acid analysis showed the presence of more acidic amino acids, wi ... | 1994 | 8028590 |
a novel family of small cysteine-rich antimicrobial peptides from seed of impatiens balsamina is derived from a single precursor protein. | four closely related peptides were isolated from seed of impatiens balsamina and were shown to be inhibitory to the growth of a range of fungi and bacteria, while not being cytotoxic to cultured human cells. the peptides, designated ib-amp1, ib-amp2, ib-amp3, and ib-amp4, are 20 amino acids long and are the smallest plant-derived antimicrobial peptides isolated to date. the ib-amps (i. balsamina antimicrobial peptides) are highly basic and contain four cysteine residues which form two intramolec ... | 1997 | 9305910 |
the induction and maintenance of flowering in impatiens. | the mechanisms that establish the floral meristem are now becoming clearer, but the way in which flowering is maintained is less well understood. impatiens balsamina provides a unique opportunity to address this question because reversion to vegetative growth can be obtained in a predictable way by transferring plants from inductive to non-inductive conditions. following increasing amounts of induction, reversion takes place at progressively later stages of flower development. partial flower ind ... | 1997 | 9310329 |
structural studies of impatiens balsamina antimicrobial protein (ib-amp1). | structural studies of ib-amp1, a small antimicrobial peptide derived from the seeds of impatiens balsamina have been performed using circular dichroism (cd) and two-dimensional proton nuclear magnetic resonance (1h nmr). this 20-residue peptide is highly basic with five arginine residues and contains four cysteines which form two intramolecular disulfide bonds. cd results reveal that the peptide may include a beta-turn but do not show evidence for either helical or beta-sheet structure over a ra ... | 1998 | 9454588 |
antipruritic dinaphthofuran-7,12-dione derivatives from the pericarp of impatiens balsamina. | dinaphthofuran-7,12-dione derivatives named balsaminones a (1) and b (2) were isolated from the pericarp of impatiens balsamina l. together with the known compound 2-methoxy-1,4-naphthoquinone (3). their structures were elucidated by spectral techniques. these compounds have significant antipruritic activity. | 1998 | 9748380 |
quantitative control of inflorescence formation in impatiens balsamina | we analyzed the process of inflorescence formation in impatiens balsamina by studying the architecture of the plant under different photoperiod treatments. floral reversion under noninductive conditions in this species is caused by the lack of persistence of the induced state in the leaf. this can be used to control the amount of inductive signal and to examine its quantitative influence on morphological changes in the plant. the floral transition was characterized by a continuum of variation at ... | 1998 | 9847093 |
screening method for paf antagonist substances: on the phenolic compounds from impatients balsamina l. | a blood pressure monitoring system was developed for studying antagonist effects against platelet activating factor (paf), a chemical mediator of anaphylactic hypotension. using this system, we were able to determine the inhibitory effects of a 35% ethanol extract (ib) from the petals of impatiens balsamina l. against paf-induced hypotension and the active principal compounds from ib. one mechanism of the antianaphylactic effects of ib was characterized as paf-antagonist effects. | 1999 | 10479766 |
biosynthetic origin of conjugated double bonds: production of fatty acid components of high-value drying oils in transgenic soybean embryos. | vegetable oils that contain fatty acids with conjugated double bonds, such as tung oil, are valuable drying agents in paints, varnishes, and inks. although several reaction mechanisms have been proposed, little is known of the biosynthetic origin of conjugated double bonds in plant fatty acids. an expressed sequence tag (est) approach was undertaken to characterize the enzymatic basis for the formation of the conjugated double bonds of alpha-eleostearic (18:3delta(9cis, 11trans,13trans)) and alp ... | 1999 | 10536026 |
comet assay application in environmental monitoring: dna damage in human leukocytes and plant cells in comparison with bacterial and yeast tests. | urban airborne particulate is a complex mixture of air pollutants, many of which have not been identified. however, short-term mutagenesis tests together with chemicophysical parameter analysis are able to better assess air quality and genotoxic load. the findings of continuous monitoring (january 1991-august 1998) of urban air genotoxicity of a po valley town (italy) on salmonella typhimurium and saccharomyces cerevisiae are reported. during this period, various measures (catalytic devices, unl ... | 1999 | 10567029 |
testosterone 5alpha-reductase inhibitor bisnaphthoquinone derivative from impatiens balsamina. | the 35% etoh extract of aerial parts of impatiens balsamina l. has been investigated for activity against testosterone 5alpha-reductase. activity-guided fractionation led to the identification of the bisnaphthquione derivative named impatienol (1), 3-hydroxy-2-¿[3-hydroxy-1,4-dioxo (2-naphthyl)] ethyl¿ naphthalene-1, 4-dione, which exhibited significant testosterone 5alpha-reductase inhibitory activity. this 5alpha-reductase inhibitory compound has been previously synthesized, but this is the fi ... | 2000 | 10641051 |
a leaf-derived signal is a quantitative determinant of floral form in impatiens. | the completion of flower development in impatiens balsamina requires continuous inductive (short-day) conditions. we have previously shown that a leaf-derived signal has a role in floral maintenance. the research described here analyzes the role of the leaf in flower development. leaf removal treatments, in which plants were restricted to a specified number of leaves, resulted in flowers with increased petal number, up to double that of the undefoliated control. similar petal number increases (a ... | 2000 | 11041880 |
separation of kaempferols in impatients balsamina flowers by capillary electrophoresis with electrochemical detection. | capillary electrophoresis with wall-jet amperometric detection was used to detect kaempferol and its derivatives kaempferol-3-glucoside, kaempferol-3-glucosylrhamnoside and kaempferol-3-(p-coumaroyl)glucoside. the influence of buffer ph on separation was investigated and optimized. with a phosphate buffer at ph 7.5, nearly complete separation of the four kaempferols was achieved according to their different electrophoretic mobilities. the detection potential was also evaluated and optimized. at ... | 2001 | 11269529 |
antipruritic and antidermatitic effect of extract and compounds of impatiens balsamina l. in atopic dermatitis model nc mice. | we examined the effects of a 35% ethanol extract (ib) from the petals of impatiens balsamina l. and the principal active compounds from ib on chronic and serious pruritus and the development of dermatitis using nc mice, a model of atopic dermatitis. ib at 100 mg/kg significantly inhibited serious scratching behaviour in the nc mouse with established dermatitis when administered i.v. 1 h before, or p.o. 24 h before the measurement. a 10 microg/kg dose of kaempferol 3-rutinoside and 2-hydroxy-1,4- ... | 2001 | 11536380 |
isolation of an antimicrobial compound from impatiens balsamina l. using bioassay-guided fractionation. | by using brine shrimp (artemia salina) lethality test-guided fractionation, a single bioactive compound (lc(50)=26 ppm) was isolated from the 95% ethanol extract of the dried aerial parts of impatiens balsamina l. and subsequently identified as 2-methoxy-1,4-naphthoquinone (mnq). the structure of mnq was confirmed by uv, ft-ir, ms, and 1-and 2-d nmr spectroscopy. the antimicrobial activity of mnq was evaluated using 12 bacterial and eight fungal strains. five gram-positive and two gram-negative ... | 2001 | 11746859 |
transgenic expression in arabidopsis of a polyprotein construct leading to production of two different antimicrobial proteins. | we developed a method for expression in arabidopsis of a transgene encoding a cleavable chimeric polyprotein. the polyprotein precursor consists of a leader peptide and two different antimicrobial proteins (amps), dmamp1 originating from dahlia merckii seeds and rsafp2 originating from raphanus sativus seeds, which are linked by an intervening sequence ("linker peptide") originating from a natural polyprotein occurring in seed of impatiens balsamina. the chimeric polyprotein was found to be clea ... | 2002 | 11950983 |
preventive effects of impatiens balsamina on the hen egg-white lysozyme (hel)-induced decrease in blood flow. | monitoring the blood flow of unanesthesized mice was found to be a reliable and effective method for studying their anaphylactic responses, in addition to the known method of monitoring blood pressure. hen egg-white lysozyme (hel)-specific anaphylaxis in mice was estimated by monitoring the decrease in blood flow with a doppler blood flow meter. this method is convenient for searching for both anaphylaxis and anti-anaphylactic substances from natural products. using this system, we estimated the ... | 2002 | 11995933 |
cyclooxygenase-2 inhibitory 1,4-naphthoquinones from impatiens balsamina l. | significant selective cyclooxygenase-2 (cox-2) inhibitory activities were observed for two new 1,4-naphthoquinone sodium salts, sodium 3-hydroxide-2[[sodium 3-hydroxide-1,4-dioxo(2-naphthyl)]ethyl]naphthalene-1,4-dione (impatienolate) (1) and sodium 2-hydroxide-3-(2-hydroxyethyl)naphthalene-1,4-dione (balsaminolate) (2), which were isolated from the corolla of impatiens balsamina l. (balsaminaceae). their structures were elucidated by spectral techniques. our results offer evidence supporting th ... | 2002 | 12033510 |
evaluation of the genotoxicity induced by the fungicide fenarimol in mammalian and plant cells by use of the single-cell gel electrophoresis assay. | fenarimol, a systemic pyrimidine carbinol fungicide, is considered to be not genotoxic or weakly genotoxic, although the available toxicological data are controversial and incomplete. our results obtained in vitro with leukocytes of two different rodent species (rat and mouse) show that fenarimol affects dna, as detected by the single-cell gel electrophoresis (scge, comet) assay. this fungicide is able to induce dna damage in a dose-related manner, with significant effectiveness at 36 nm, but wi ... | 2003 | 12972058 |
the flavonols of impatiens balsamina l. | | 1958 | 13560021 |
the isolation of 2-hydroxy-1,4-naphthoquinone from impatiens balsamina l. (balsaminaceae). | | 1965 | 14330360 |
antifungal activity of synthetic peptides derived from impatiens balsamina antimicrobial peptides ib-amp1 and ib-amp4. | seeds of impatiens balsamina contain a set of related antimicrobial peptides (ib-amps). we have produced a synthetic variant of ib-amp1, oxidized to the bicyclic native conformation, which was fully active on yeast and fungal strains; and four linear 20-mer ib-amp variants, including two all-d forms. we show that the all-d variants are as active on yeast and fungal strains as native peptides. in addition, fungal growth inhibition nor salt-dependency of ib-amp4 could be improved by more than two- ... | 2005 | 15949628 |
mechanisms and function of flower and inflorescence reversion. | flower and inflorescence reversion involve a switch from floral development back to vegetative development, thus rendering flowering a phase in an ongoing growth pattern rather than a terminal act of the meristem. although it can be considered an unusual event, reversion raises questions about the nature and function of flowering. it is linked to environmental conditions and is most often a response to conditions opposite to those that induce flowering. research on molecular genetic mechanisms u ... | 2005 | 16131510 |
fungicidal and binding properties of three plant peptides. | the fungicidal properties of plant seed peptides from heuchera sanginea (hs-afp1), raphanus sativus (ea-afp2), and impatiens balsamina (ib-amp3) were determined for the non-germinated and germinated conidia of aspergillus flavus and fusarium moniliforme. these peptides were weakly lethal for germinated but not for non-germinated conidia of a. flavus. both non-germinated and germinated conidia of f. moniliforme were susceptible to these peptides. overall, f. moniliforme was more susceptible than ... | 1998 | 16284838 |
leafy, terminal flower1 and agamous are functionally conserved but do not regulate terminal flowering and floral determinacy in impatiens balsamina. | in impatiens balsamina a lack of commitment of the meristem during floral development leads to the continuous requirement for a leaf-derived floral signal. in the absence of this signal the meristem reverts to leaf production. current models for arabidopsis state that leafy (lfy) is central to the integration of floral signals and regulates flowering partly via interactions with terminal flower1 (tfl1) and agamous (ag). here we describe impatiens homologues of lfy, tfl1 and ag (iblfy, ibtfl1 and ... | 2005 | 16359391 |
anthocyanin production in detached petals of impatiens balsamina l. | | 1961 | 16655455 |
certain properties of hypocotyl of impatiens balsamina reflecting physiological complexity. | | 1961 | 16655569 |
chemical factors affecting anthocyanin formation and morphogenesis in cultured hypocotyl segments of impatiens balsamina. | | 1964 | 16655918 |
the differentiation of pigmentation in flower parts, iv. flavonoid elaborating enzymes from petals of impatiens balsamina s. | extracts of the flower petals of impatiens balsamina l. contain enzymes which catalyze the glycosylation of phenolic compounds. enzymes have been extracted which glycosylate hydroquinone to arbutin and at least 3 different flavonols to the 3-monoglucoside. the hydroquinone glucosylating enzyme is similar to enzymes previously described except that it requires an unidentified low molecular weight cofactor. the glucosylation of flavonols follows normal enzyme kinetics; it requires a nucleotide dip ... | 1968 | 16656918 |
floral meristem indeterminacy depends on flower position and is facilitated by acarpellate gynoecium development in impatiens balsamina. | * floral meristems are generally determinate. termination of their activity varies with species, occurring after carpel or ovule development, depending on the placentation type. in terminal flowering impatiens balsamina (cv. dwarf bush flowered) some flowers exhibit meristem indeterminacy; they produce organs from the placenta after ovule development. * here we provide a detailed description of gynoecium development in this line and explore the basis of the indeterminate nature of some of its fl ... | 2007 | 17176395 |
impatiens balsamina l.-the inheritance of floral colors. | | 1958 | 17247739 |
chemical aspects of the inheritance of flower color in impatiens balsamina l. | | 1958 | 17247741 |
influence of genes controlling flower color on relative quantities of anthocyanins and flavonols in petals of impatiens balsamina. | | 1959 | 17247857 |
a new biscoumarin from impatiens balsamina root cultures. | a new biscoumarin, 4,4'-biisofraxidin has been isolated from the root cultures of impatiens balsamina (balsaminaceae). the structure was elucidated by 1d and 2d nmr techniques. | 1998 | 17253327 |
in vitro activity of kaempferol isolated from the impatiens balsamina alone and in combination with erythromycin or clindamycin against propionibacterium acnes. | the in vitro antibacterial activity against antibiotic-resistant propionibacterium acnes of kaempferol isolated from the impatiens balsamina alone and in combination with erythromycin or clindamycin antibiotics was investigated. the antibiotic combination effect against antibiotic-resistant p. acnes was studied by checkerboard test. kaempferol and quercetin demonstrated antibacterial activities against p. acnes. minimum inhibitory concentrations (mics) for both compounds were < or =32 ug/ml and ... | 2007 | 17978809 |
isolation and identification of an anti-tumor component from leaves of impatiens balsamina. | we have previously shown that ethanol or chloroform extracts of the leaves of impatiens balsamina (lib) have anti-tumor activity against the human hepatocellular carcinoma cell line hepg2. the ethanol extracts were separated into five fractions according to polarity. an mtt assay indicated that two of the fractions had anti-tumor activity and that the petroleum ether fraction (pef) was the most active. but the available quantities of both the pef and chloroform fractions (chf) were limited, prec ... | 2008 | 18305414 |
time-activity budgets and behaviour of the amazilia hummingbird, amazilia amazilia (apodiformes: trochilidae) in an urban environment. | this study deals with the time-activity budgets of amazilia amazilia, a territorial hummingbird, and its preferences for different flower species and perches in the gardens of lima (peru) in september 2001. a. amazilia spent an important part of its time resting on perches (ca. 80%) and only 15.5% for foraging, devoted essentially to flower visitation and only 0.3% for hunting and drinking water. territorial defence accounted for 2% of total time, mostly against coereba flaveola, an introduced n ... | 2006 | 18491628 |
suppression of rhizoctonia solani on impatiens by enhanced microbial activity in composted swine waste-amended potting mixes. | abstract peat moss-based potting mix was amended with either of two composted swine wastes, csw1 and csw2, at rates from 4 to 20% (vol/vol) to evaluate suppression of pre-emergence damping-off of impatiens (impatiens balsamina) caused by rhizoctonia solani (anastomosis group-4). a cucumber bioassay was used prior to each impatiens experiment to monitor maturity of compost as the compost aged in a curing pile by evaluating disease suppression toward both pythium ultimum and r. solani. at 16, 24, ... | 2003 | 18944095 |
early stages of nematode-induced giant-cell formation in roots of impatiens balsamina. | giant cells induced in roots of impatiens balsamina by meloidogyne javanica and meloidogyne incognita have been examined by light and electron microscopy. the first sign of giant-cell formation was division of cells surrounding a larva. cell plate alignment appeared to proceed normally, but cytokinesis was unsuccessful and binucleate cells formed subsequently. no wall breakdown was evident then or later. the number of nuclei appeared to increase by repeated mitosis without separation by cytokine ... | 1978 | 19305816 |
genome analysis and characterization of a tobacco mosaic virus isolate infecting balsam (impatiens balsamina). | the complete rna genomic sequence of a tobacco mosaic virus isolate infecting impatiens balsamina, designated as tmv-im, has been determined. the genomic sequence and the predicted gene products of tmv-im were similar to those of other members of the genus tobamovirus. the cp orf of tmv-im showed sequence identities of 95.0-99.5% with the corresponding orfs of other tmv strains. full-length cdna of tmv-im was amplified by rt-pcr with a 5'-end primer harboring a t7 promoter sequence and a 3'-end ... | 2009 | 19381775 |
in vitro activity of impatiens balsamina l. against multiple antibiotic-resistant helicobacter pylori. | impatiens balsamina l. has been used as indigenous medicine in asia for the treatment of rheumatism, fractures, and fingernail inflammation. in this study, anti-h. pylori activity of i. balsamina l. was investigated. the mics, mbcs, time-kill assay, and effect of environmental ph for the plant extracts were determined. the test h. pylori strains have resistance to clarithromycin (clr), metronidazole (mtz), and levofloxacin (lvx). from our results, all part (root/stem/leaf, seed, and pod) extract ... | 2009 | 19655409 |
in vitro activity of 2-methoxy-1,4-naphthoquinone and stigmasta-7,22-diene-3{beta}-ol from impatiens balsamina l. against multiple antibiotic-resistant helicobacter pylori. | infection with helicobacter pylori is strongly associated with gastric cancer and gastric adenocarcinoma. who classified h. pylori as a group 1 carcinogen in 1994. impatiens balsamina l. has been used as indigenous medicine in asia for the treatment of rheumatism, fractures and fingernail inflammation. in this study, we isolated anti-h. pylori compounds from this plant and investigated their anti- and bactericidal activity. compounds of 2-methoxy-1,4-naphthoquinone (meonq) and stigmasta-7,22-die ... | 2009 | 19773391 |
antimicrobial specificity and mechanism of action of disulfide-removed linear analogs of the plant-derived cys-rich antimicrobial peptide ib-amp1. | ib-amp1 is a 20-residue disulfide-linked beta-sheet antimicrobial peptide found in the seeds of impatiens balsamina. in order to investigate the effects of the 2 disulfide bonds on the antimicrobial specificity, to determine the mechanism of antimicrobial action of ib-amp1 and to develop novel cell-selective antimicrobial peptides with improved antimicrobial specificity as compared to wild-type ib-amp1, we synthesized a disulfide-removed linear analog of ib-amp1 with l-pro, d-pro or peptoid resi ... | 2009 | 19778562 |
fertilizer efficiency and environmental risk of irrigating impatiens with composting leachate in decentralized solid waste management. | the reduction and reuse of composting leachate is an issue of importance in the field of decentralized solid waste management. in this study, composting leachate from source-separated food waste was treated and subsequently used as liquid fertilizer to irrigate impatiens (impatiens balsamina). the leachate was altered by adjusting storage time and dilution, and through addition of microbial inocula. for each test case, the effects of irrigation were monitored by analyzing the impatiens extension ... | 2010 | 20194007 |
increased production of naphthoquinones in impatiens balsamina root cultures by elicitation with methyl jasmonate. | impatiens balsamina root cultures were treated with yeast extract (ye), candida albicans homogenate (cah), trichophyton rubrum homogenate (trh), chitosan (chi) and methyl jasmonate (mj). different elicitors, depending on concentrations used exerted differential effects on the production of the three main naphthoquinones, lawsone (2-hydroxy-1,4-naphthoquinone), lawsone methyl ether and methylene-3,3'-bilawsone. treatment with mj (400microm) was capable of increasing production of lawsone, and law ... | 2010 | 20620051 |
promoted biodegradation and microbiological effects of petroleum hydrocarbons by impatiens balsamina l. with strong endurance. | phytoremediation is a promising green technology for cleanup of petroleum hydrocarbons (phcs) in contaminated environment. based on the objective of identifying special ornamental plants for the effective biodegradation of phcs, the efficacy of impatiens balsamina l. to phytoremedy petroleum contaminated soil from the shengli oil field in dongying city, shandong province, china, was further examined in a field plot-culture experiment under greenhouse conditions. after a 4-month culture period, t ... | 2010 | 20724074 |
2-methoxy-1,4-naphthoquinone isolated from impatiens balsamina in a screening program for activity to inhibit wnt signaling. | a screening study using a luciferase assay to identify natural products which inhibit wnt signaling was carried out. the bioassay-guided fractionation of aerial parts of a plant, impatiens balsamina, led to the isolation of 2-methoxy-1,4-naphthoquinone (1) as an active compound. compound 1 inhibited the tcf/β-catenin (top) transcriptional activity (ic(50) 2.9 µm), while it decreased the transcriptional activity of fop (mutated tcf-binding site)-transfected cells at >5 µm. | 2010 | 20886301 |
simultaneous determination of three naphthoquinones in the leaves of impatiens balsamina l. by reversed-phase high-performance liquid chromatography. | naphthoquinones; lawsone (1), lawsone methyl ether (2) and methylene-3,3'-bilawsone (3) are the main active compounds of impatiens balsamina leaves. | 2010 | 20931623 |
simultaneous qualification and quantification of baccharane glycosides in impatientis semen by hplc-esi-msd and hplc-elsd. | this study presents a high performance liquid chromatography (hplc) with electrospray ionization mass spectrometric detection (esi-msd) and evaporative light scattering detection (elsd) method for the simultaneous qualification and quantification of eight major baccharane glycosides, namely hosenlosides a, b, c, f, g, k, l, and m in impatientis semen, a chinese herbal medicine derived from the seeds of impatiens balsamina l. in order to achieve optimum performance, several extraction parameters ... | 2010 | 21075577 |
two new flavone glycosides from the seeds of impatiens balsamina l. | two new flavone glycosides were isolated from the seeds of impatiens balsamina l. and their structures were determined as quercetin-3-o-[α-l-rhamnose-(1 → 2)-β-d-glucopyranosyl]-5-o-β-d-glucopyranoside (1), and quercetin-3-o-[(6'''-o-caffeoyl)-α-l-rhamnose-(1 → 2)-β-d-glucopyranosyl]-5-o-β-d-glucopyranoside (2) on the basis of various spectral and chemical studies. | 2010 | 21128143 |
structure and development of the upper haustorium in the parasitic flowering plant cuscuta japonica (convolvulaceae). | the anatomical and ultrastructural development of the haustorium of the cuscuta japonica, a holoparasitic angiosperm, growing on the host plant impatiens balsamina was studied. after the shoot tips of light-grown parasite seedlings contacted the host, the upper haustorium (external to the host organ) developed through three main successive stages of the haustorial initials, the meristem, and the endophyte primoridium (ep) within the middle layer of the cortex of the parasite stem. the haustorial ... | 2007 | 21636442 |
2-meth-oxy-naphthalene-1,4-dione. | the title compound, c(11)h(8)o(3), was isolated from impatiens balsamina plants (balsam, lib) grown in our laboratory. the two six-membered rings of the naphthalene-1,4-dione unit are coplanar [maximum deviation = 0.009 (1) å]. the o and c atoms of the meth-oxy substituent also lie close to the naphthalene plane, with deviations of 0.0090 (2) and 0.047 (2) å, respectively. | 2011 | 21754213 |
comparison of antimicrobial activities of naphthoquinones from impatiens balsamina. | lawsone (1), lawsone methyl ether (2), and methylene-3,3'-bilawsone (3) are the main naphthoquinones in the leaf extracts of impatiens balsamina l. (balsaminaceae). antimicrobial activities of these three naphthoquinones against dermatophyte fungi, yeast, aerobic bacteria and facultative anaerobic and anaerobic bacteria were evaluated by determination of minimal inhibitory concentrations (mics) and minimal bactericidal or fungicidal concentrations (mbcs or mfcs) using a modified agar dilution me ... | 2011 | 21895457 |
bacterial reduction of cr(vi) at technical scale--the malaysian experience. | the bacterial reduction of cr(vi) from industrial wastewater was evaluated using a 2.0-m(3) bioreactor. liquid pineapple waste was used as a nutrient for the biofilm community formed inside the bioreactor. the use of rubber wood sawdust as packing material was able to immobilize more than 10(6) cfu ml(-1) of acinetobacter haemolyticus cells after 3 days of contact time. complete reduction of 15-240 mg l(-1) of cr(vi) was achieved even after 3 months of bioreactor operation. cr(vi) was not detect ... | 2012 | 22350941 |
tandem combination of trigonella foenum-graecum defensin (tfgd2) and raphanus sativus antifungal protein (rsafp2) generates a more potent antifungal protein. | plant defensins are small (45 to 54 amino acids) positively charged antimicrobial peptides produced by the plant species, which can inhibit the growth of a broad range of fungi at micro-molar concentrations. these basic peptides share a common characteristic three-dimensional folding pattern with one α-helix and three β-sheets that are stabilized by eight disulfide-linked cysteine residues. instead of using two single-gene constructs, it is beneficial when two effective genes are made into a sin ... | 2013 | 24022215 |
antibacterial activity of the recombinant antimicrobial peptide ib-amp4 from impatiens balsamina and its synergy with other antimicrobial agents against drug resistant bacteria. | ib-amp4 is an antimicrobial peptide of impatiens balsamina (balsaminaceae). ib-amp4 was produced as a recombinant peptide and in this study its antimicrobial activity against human bacterial pathogens was investigated. ib-amp4 was bactericidal against both gram positive and gram negative bacteria with mic values between 0.49 and 3.5 microm in sensitive species. a genuine synergistic effect was achieved when ib-amp4 was employed in combination with the plant monoterpene thymol against drug-resist ... | 2013 | 23923648 |
bactericidal properties of the antimicrobial peptide ib-amp4 from impatiens balsamina produced as a recombinant fusion-protein in escherichia coli. | antimicrobial peptides (amps) represent a novel class of powerful natural antimicrobial agents. as amps are bactericidal, production of amps in recombinant bacteria is far from trivial. we report the production of impatiens balsamina antimicrobial peptide 4 (ib-amp4, originally isolated from impatiens balsamina) in escherichia coli as a fusion protein and investigate ib-amp4's antimicrobial effects on human pathogens. a plasmid vector pet32a-trx-ib-amp4 was constructed and transferred into e. co ... | 2013 | 23713064 |
processing and targeting of proteins derived from polyprotein with 2a and lp4/2a as peptide linkers in a maize expression system. | in the transformation of multiple genes, gene fusion is an attractive alternative to other methods, including sexual crossing, re-transformation, and co-transformation, among others. the 2a peptide from the foot-and-mouth disease virus (fmdv) causes the co-translational "cleavage" of polyprotein and operates in a wide variety of eukaryotic cells. lp4, a linker peptide that originates from a natural polyprotein occurring in the seed of impatiens balsamina, can be split between the first and secon ... | 2017 | 28358924 |
anti-gastric adenocarcinoma activity of 2-methoxy-1,4-naphthoquinone, an anti-helicobacter pylori compound from impatiens balsamina l. | 2-methoxy-1,4-naphthoquinone (meonq) from impatiens balsamina l. exhibited strong anti-h. pylori activity in our previous study. in this study, we investigated the cytotoxicity of meonq against gastric adenocarcinoma (mkn45 cell line) and propose the relevant mechanisms. meonq resulted in serious necrosis via superoxide anion catastrophe when the treatment doses were higher than 50μm, whereas apoptosis occurred at low treatment doses (25-50μm) through the caspase-dependent apoptosis pathway. nec ... | 2012 | 22516543 |
three-dimensional ultrastructure of feeding tubes and interconnected endoplasmic reticulum in root-knot nematode-induced giant cells in rose balsam. | we investigated the three-dimensional ultrastructure of feeding tubes and the surrounding region in giant cells induced in rose balsam (impatiens balsamina l.) roots by the root-knot nematode meloidogyne incognita, using osmium maceration coupled with field emission scanning electron microscopy (fe-sem). in the roots of 35-day-old galled rose balsam plants, adult nematodes induced the formation of giant cells containing feeding tubes and numerous organelles, including tubular endoplasmic reticul ... | 2017 | 28204899 |
the feeding behavior of adult root-knot nematodes (meloidogyne incognita) in rose balsam and tomato. | meloidogyne incognita is a parasitic root-knot nematode that causes considerable yield loss in a wide range of plants. in this study we documented the movement of adult female nematodes for more than 2 hr in micro-slices of infected tomato (solanum lycopersicum) and rose balsam (impatiens balsamina) plants using light and video microscopy. stylet thrusting was followed by short pumping actions of the esophagus, dorsal esophageal gland ampulla, and metacorpal bulb. regular thrusting was normally ... | 2014 | 25276004 |
its2 barcoding dna region combined with high resolution melting (hrm) analysis of hyoscyami semen, the mature seed of hyoscyamus niger. | hyoscyami semen, the mature dried seed of hyoscyamus niger l., has long been used as a traditional chinese medicine to treat human diseases. hyoscyami semen is found in local markets in china. in markets, sellers and buyers commonly inadvertently mix the seeds of h. niger with the seeds of related species such as hygrophila salicifolia (vahl) nees, astragalus complanatus r. br., cuscuta australis r. br., cuscuta chinensis lam., and impatiens balsamina l. because of their similar morphologies or ... | 2016 | 28262116 |
enhancement of the initial growth rate of agricultural plants by using static magnetic fields. | electronic devices and high-voltage wires induce magnetic fields. a magnetic field of 1,300-2,500 gauss (0.2 tesla) was applied to petri dishes containing seeds of garden balsam (impatiens balsamina), mizuna (brassica rapa var. japonica), komatsuna (brassica rapa var. perviridis), and mescluns (lepidium sativum). we applied magnets under the culture dish. during the 4 days of application, we observed that the stem and root length increased. the group subjected to magnetic field treatment (n = 10 ... | 2016 | 27500712 |
preference of a polyphagous mirid bug, apolygus lucorum (meyer-dür) for flowering host plants. | apolygus lucorum (meyer-dür) (hemiptera: miridae) is one of the most important herbivores in a broad range of cultivated plants, including cotton, cereals, vegetables, and fruit crops in china. in this manuscript, we report on a 6-year long study in which (adult) a. lucorum abundance was recorded on 174 plant species from 39 families from early july to mid-september. through the study period per year, the proportion of flowering plants exploited by adult a. lucorum was significantly greater than ... | 2013 | 23874835 |
a new label-free screen for steroid 5α-reductase inhibitors using lc-ms. | steroid 5α-reductase (s5αr) plays an important role in metabolizing testosterone into active androgen dihydrotestosterone (dht) which is involved in many androgen dependent disorders, such as androgenic alopecia, benign prostatic hyperplasia and acne. the method for screening for s5αr inhibition is key in finding new antagonists. in this study, the label-free s5αr inhibitory assay using lc-ms was developed. s5αr type 1 enzyme was obtained from lncap prostate cancer cells. the enzymatic assay was ... | 2016 | 27789379 |
a bioinformatics approach to distinguish plant parasite and host transcriptomes in interface tissue by classifying rna-seq reads. | the genus cuscuta is a group of parasitic plants that are distributed world-wide. the process of parasitization starts with a cuscuta plant coiling around the host stem. the parasite's haustorial organs then establish a vascular connection allowing for access to the phloem content. the host and the parasite form new cellular connections, suggesting coordination of developmental and biochemical processes. simultaneous monitoring of gene expression in the parasite's and host's tissues may shed lig ... | 2015 | 26052341 |