Publications

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the occurrence of cyanobacteria in pulp and paper waste-treatment systems.pulp and paper secondary waste-treatment systems in brazil, canada, new zealand, and the u.s.a. contained dynamic cyanobacterial communities, some of which exceeded heterotrophic bacterial biomass. no other viable photoautotrophic populations were detected in the ponds. regardless of geographical location, oscillatoriales including phormidium, geitlerinema, and pseudanabaena were the dominant taxa. as well, chroococcus (chroococcales) was an important genus in brazil and new zealand. the possibl ...200111575503
cyanobacterial diversity in extreme environments in baja california, mexico: a polyphasic study.cyanobacterial diversity from two geographical areas of baja california sur, mexico, were studied: bahia concepcion, and ensenada de aripez. the sites included hypersaline ecosystems, sea bottom, hydrothermal springs, and a shrimp farm. in this report we describe four new morphotypes, two are marine epilithic from bahia concepcion, dermocarpa sp. and hyella sp. the third, geitlerinema sp., occurs in thermal springs and in shrimp ponds, and the fourth, tychonema sp., is from a shrimp pond. the pa ...200112051567
genotypic diversity of oscillatoriacean strains belonging to the genera geitlerinema and spirulina determined by 16s rdna restriction analysis.genotypic diversity of several cyanobacterial strains mostly isolated from marine or brackish waters, belonging to the genera geitlerinema and spirulina, was investigated by amplified 16s ribosomal dna restriction analysis and compared with morphological features and response to salinity. cluster analysis was performed on amplified 16s rdna restriction profiles of these strains along with profiles obtained from sequence data of five spirulina-like strains, including three representatives of the ...200312732964
the presence of the cyanobacterial toxin microcystin in black band disease of corals.black band disease (bbd) is a migrating, cyanobacterial dominated, sulfide-rich microbial mat that moves across coral colonies lysing coral tissue. while it is known that bbd sulfate-reducing bacteria contribute to bbd pathogenicity by production of sulfide, additional mechanisms of toxicity may be involved. using hplc/ms, the cyanotoxin microcystin was detected in 22 field samples of bbd collected from five coral species on nine reefs of the wider caribbean (florida keys and bahamas). two cyano ...200717506829
molecular detection and ecological significance of the cyanobacterial genera geitlerinema and leptolyngbya in black band disease of corals.black band disease (bbd) is a pathogenic, sulfide-rich microbial mat dominated by filamentous cyanobacteria that infect corals worldwide. we isolated cyanobacteria from bbd into culture, confirmed their presence in the bbd community by using denaturing gradient gel electrophoresis (dgge), and demonstrated their ecological significance in terms of physiological sulfide tolerance and photosynthesis-versus-irradiance values. twenty-nine bbd samples were collected from nine host coral species, four ...200717601818
cyanobacterial diversity and halotolerance in a variable hypersaline environment.the great salt plains (gsp) in north-central oklahoma, usa is an expansive salt flat (approximately 65 km(2)) that is part of the federally protected salt plains national wildlife refuge. the gsp serves as an ideal environment to study the microbial diversity of a terrestrial, hypersaline system that experiences wide fluctuations in freshwater influx and diel temperature. our study assessed cyanobacterial diversity at the gsp by focusing on the taxonomic and physiological diversity of gsp isolat ...200817653786
different genotypes of anatoxin-producing cyanobacteria coexist in the tarn river, france.repeated dog deaths occurred in 2002, 2003, and 2005 after the animals drank water from the shoreline of the tarn river in southern france. signs of intoxication indicated acute poisoning due to a neurotoxin. floating scum and biofilms covering pebbles were collected in the summers of 2005 and 2006 from six different sites along 30 km from the border of this river. the cyanobacterial neurotoxic alkaloid anatoxin-a and/or its methyl homolog, homoanatoxin-a, was detected in the extracts of most sa ...200717933923
cyanobacterial composition of microbial mats from an australian thermal spring: a polyphasic evaluation.cyanobacterial composition of microbial mats from an alkaline thermal spring issuing at 43-71 degrees c from tropical north-eastern australia are described using a polyphasic approach. eight genera and 10 species from three cyanobacterial orders were identified based on morphological characters. these represented taxa previously known as thermophilic from other continents. ultrastructural analysis of the tower mats revealed two filamentous morphotypes contributed the majority of the biomass. bot ...200818081588
adaptation of cyanobacteria to the sulfide-rich microenvironment of black band disease of coral.black band disease (bbd) is a cyanobacteria-dominated microbial mat that migrates across living coral colonies lysing coral tissue and leaving behind exposed coral skeleton. the mat is sulfide-rich due to the presence of sulfate-reducing bacteria, integral members of the bbd microbial community, and the sulfide they produce is lethal to corals. the effect of sulfide, normally toxic to cyanobacteria, on the photosynthetic capabilities of five bbd cyanobacterial isolates of the genera geitlerinema ...200919049501
determining the specific microbial populations and their spatial distribution within the stromatolite ecosystem of shark bay.the stromatolites at shark bay, western australia, are analogues of some of the oldest evidence of life on earth. the aim of this study was to identify and spatially characterize the specific microbial communities associated with shark bay intertidal columnar stromatolites. conventional culturing methods and construction of 16s rdna clone libraries from community genomic dna with both universal and specific pcr primers were employed. the estimated coverage, richness and diversity of stromatolite ...200919092864
cyanotoxins from black band disease of corals and from other coral reef environments.many cyanobacteria produce cyanotoxins, which has been well documented from freshwater environments but not investigated to the same extent in marine environments. cyanobacteria are an obligate component of the polymicrobial disease of corals known as black band disease (bbd). cyanotoxins were previously shown to be present in field samples of bbd and in a limited number of bbd cyanobacterial cultures. these toxins were suggested as one of the mechanisms contributing to bbd-associated coral tiss ...200919554362
novel toxic effects associated with a tropical limnothrix/geitlerinema-like cyanobacterium.the presence of a toxic strain of a fine filamentous cyanobacterium belonging to the oscillatorialean family pseudanabaenacea was detected during a survey of cyanobacterial taxa associated with the presence of cylindrospermopsin in dams in central queensland (australia). the strain, ac0243, was isolated and cultured, its genomic dna extracted and 16s rna gene sequenced. phylogenetic analysis placed ac0243 with limnothrix species, although this genus appears polyphyletic. moreover, not all morpho ...201119950362
microcystin production and ecological physiology of caribbean black band disease cyanobacteria.molecular studies of black band disease (bbd), a coral disease found on tropical and subtropical reefs worldwide, have shown that one 16s rrna gene sequence is ubiquitous. this sequence has been reported to be a member of the cyanobacterial genus oscillatoria. in this study, extracts of two cultured laboratory strains of bbd oscillatoria, and for comparison two strains of bbd geitlerinema, all isolated from reefs of the wider caribbean, were analysed using ultra-performance liquid chromatography ...201021143569
bacterial community composition characterization of a lead-contaminated microcoleus sp. consortium.a microcoleus sp. consortium, obtained from the ebro delta microbial mat, was maintained under different conditions including uncontaminated, lead-contaminated, and acidic conditions.201121340467
vertical and temporal dynamics of cyanobacteria in the carpina potable water reservoir in northeastern brazil.this study analysed vertical and temporal variations of cyanobacteria in a potable water supply in northeastern brazil. samples were collected from four reservoir depths in the four months; september and december 2007; and march and june 2008. the water samples for the determination of nutrients and cyanobacteria were collected using a horizontal van dorn bottle. the samples were preserved in 4% formaldehyde for taxonomic analysis using an optical microscope, and water aliquots were preserved in ...201121755163
acute toxicity assessment of perfluorinated carboxylic acids towards the baltic microalgae.the presence of high-energy carbon-fluorine bonds in perfluoro compounds lends them great stability and causes them to be environmentally persistent. relatively little is known about the acute toxicity of perfluorinated carboxylic acids (pfcas) to ecotoxicological markers such as aquatic plants and animals. this study tested the toxicity of these compounds to the green alga chlorella vulgaris, the diatom skeletonema marinoi and the blue-green alga geitlerinema amphibium, which are species repres ...200921783999
Cyanobacterial blooms in stratified and destratified eutrophic reservoirs in semi-arid region of Brazil.This study investigated the dynamics of cyanobacteria in two deep, eutrophic reservoirs in a semi-arid region of Brazil during periods of stratification and destratification. Four collections were carried out at each reservoir at two depths at three-month intervals. The following abiotic variables were analyzed: water temperature, dissolved oxygen, pH, turbidity, water transparency, total phosphorus, total dissolved phosphorus, orthophosphate and total nitrogen. Phytoplankton density was quantif ...201122146960
inflammatory effects of the toxic cyanobacterium geitlerinema amphibium.toxic cyanobacteria in public water reservoirs may cause severe health issues for livestock and human beings. geitlerinema amphibium, which is frequently found in são paulo city's drinking water supplies, showed toxicity in the standard mouse bioassay, while displaying signs of intoxication and post-mortem findings different from those showed by animals intoxicated by known cyanotoxins. we report here the alterations caused by g. amphibium methanolic extract on mouse microcirculatory network, as ...201121867725
Phytoplankton abundance, dominance and coexistence in an eutrophic reservoir in the state of Pernambuco, Northeast Brazil.The present study reports the phytoplankton abundance, dominance and co-existence relationships in the eutrophic Carpina reservoir, Pernambuco, Brazil. Sampling was carried out at six different depths bimonthly at a single reservoir spanning two climatic periods: dry season (January, September, and November 2006) and rainy season (March, May, and July 2006). Density, abundance, dominance, specific diversity and equitability of the community were determined, along with chlorophyll a, and physical ...201122146959
detection of bioactive exometabolites produced by the filamentous marine cyanobacterium geitlerinema sp.marine cyanobacteria are noted for their ability to excrete metabolites with biotic properties. this paper focuses on such exometabolites obtained from the culture of the marine filamentous cyanobacterium geitlerinema sp. strain, their purification and subsequent analyses. by this means the recoveries of the active compounds, a prerequisite for properly determining their concentration, are quantified here for the first time. we demonstrate a new procedure using amberlite xad-1180 resin in combin ...201122160344
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