| effect of the composition of the medium and the conditions of aspergillus foetidus cultivation on the biosynthesis of glucoamylase. | the optimal conditions for biosynthesis of exocellular glucoamylase were found in the course of submerged cultivation of aspergillus foetidus atcc 14916: ph 4.5 at the beginning of cultivation, cultivation for four days, a temperature of 30 degrees c, and an aeration in the fermenter of 1.5 volumes of the air per 1 volume of the medium with a stirring of 280 rpm. the material can be inoculated either as spores (1 x 10(7) spores per 100 ml of the medium) or a material germinated from spores (5% b ... | 1978 | 26852 |
| [isolation and purification of glucoamylase from the submerged culture of aspergillus foetidus atcc 14916]. | isolation and purification of glucoamylase from aspergillus foetidus atcc 14916 were examined. ethanol and ammonium sulphate proved to be the best precipitants. with their use the yield of glucoamylase was 99.0 and 92.3%, respectively. treatment of the culture liquid with 0.5% cacl2 and activated charcoal clarified the filtrate noticeably and left glucoamylase activity unchanged. during submerged cultivation the gungus synthesized no glucosyl transferase. a method to inactivate alpha-amylase thr ... | 1978 | 353788 |
| semi-conservative transcription in particles of a double-stranded rna mycovirus. | during transcription in vitro catalysed by the virion rna polymerase of aspergillus foetidus virus afv-s in the presence of tritiated utp, the virus double-stranded rna becomes labelled in one strand, which has the same sequence as the single-stranded rna transcripts produced. most of the label incorporated into double-stranded rna could be chased into single stranded rna by further reaction with excess unlabelled nucleoside triphosphates. in reactions containing tritiated utp the single-strande ... | 1978 | 724502 |
| biophysical and biochemical properties of two viruses isolated from aspergillus foetidus. | aspergillus foetidus virus s (afv-s) and a. foetidus virus f(afv-f) have been shown to be serologically unrelated. amino acid compositions of the two virus capsids are compared and their capsid polypeptides have been examined by sds-polyacrylamide gel electrophoresis. afv-f contained one major (phi3) and two minor (phi1 and phi2) polypeptides with mol. wt. 87000, 125000 and 100000, while afv-s contained one major (sigma1) and one minor (sigma2) polypeptide with mol. wt. 83000 and 78000 respectiv ... | 1975 | 806661 |
| molecular weight of double-stranded rna: a re-examination of aspergillus foetidus virus s rna components. | the mol. wt of the three double-stranded rna (ds-rna) components of aspergillus foetidus virus s have been re-determined by using polyacrylamide gel electrophoresis (a) of native ds-rna with ds-rna standards and (b) of denatured ds-rna with single-stranded rna standards. good agreement between the two methods and confirmation that the relationship between log mol. wt. and electrophoretic mobility of ds-rna is not linear were obtained. the new mol. wt. values (4-0 to 4-2 x 10(6); 2-5 to 2-7 x 10( ... | 1977 | 915482 |
| an unstable strain of aspergillus foetidus segregating proline auxotrophs. | two basic colony types have been obtained through single conidial isolation from the bode strain of aspergillus foetidus as well as from mutants of this unstable strain. type i is prototrophic whereas type ii is an auxotroph requiring proline. when a type i strain is grown on complex medium it gradually becomes overwhelmed by type ii sectors of growth. however, essentially pure cultures of type i can be maintained on minimal medium (lacking proline). the yield of glucoamylase from type ii cultur ... | 1975 | 1147611 |
| rna polymerase activity in double-stranded ribonucleic acid virus particles from aspergillus foetidus. | | 1975 | 1180932 |
| [dermatomycoses in workers in enterprises of the microbiological industry]. | mycologic examination of 54 patients with clinical manifestations of dermatomycosis, engaged in glucose oxidase and catalase production has found a producer fungus, penicillium vitale in 31 (38%); 3 out of 37 people contacting with producers of glucamylase and pectofetidine appeared to be infected by aspergillus awamori (1.1%) and aspergillus foetidus (1.1%), respectively. the producers were isolated from the skin of 40 (16.44%) out of 243 workers having no clinical signs of dermatomycosis, main ... | 1992 | 1427292 |
| [development of a new system for selection of a. foetidus transformed with foreign genes by using thymidine kinase gene as a marker and expression of hbsag gene in a. foetidus]. | a new system for selection of transformed aspergillus foetidus was reported. in this system, tk- a. foetidus which were constructed by homologous recombination of mutated tk gene of vaccinia virus with tk gene of a. foetidus were screened by adding budr in agar plates. conditions for screen of tk+ a. foetidus strain, transformation of a. foetidus and selection of transformed tk- a. foetidus have been studied. by using this system, several transformed a. foetidus which contained hbsag gene derive ... | 1990 | 2360327 |
| [various characteristics of growth kinetics and exohydrolase synthesis in aspergillus foetidus and zygofabospora marxiana]. | the kinetics of growth and synthesis of exohydrolases (polygalacturonases and proteinases) were studied in aspergillus foetidus and zygofabospora marxiana. the processes of growth and synthesis of polygalacturonases were found to be shifted in time. endopolymethylgalacturonase of the micromycete and endopolygalacturonase of the yeast are not accumulated in the cells, but are mainly secreted into the growth medium. the maximal value of polygalacturonase bound to the cell coincides with the maxima ... | 1985 | 3892247 |
| antiviral activity of double stranded rna from a virus isolated from aspergillus foetidus. | | 1970 | 4316959 |
| intergenic complementation of glucoamylase and citric acid production in two species of aspergillus. | all auxotrophs of aspergillus foetidus and all but two auxotrophs of a. niger which we isolated yield glucoamylase and citric acid, respectively, at levels below that of the prototrophic strain from which they were derived. results of representative heterokaryon tests suggest that the nucleus was principally responsible for the inheritance of citric acid or glucoamylase production. most somatic diploid strains of a. foetidus gave rise to higher yields of glucoamylase when compared to their haplo ... | 1973 | 4716723 |
| purine ribonucleosidase g from aspergillus foetidus. | nucleosidase g was prepared by growing aspergillus foetidus on bran, and was purified by passage through a diethylaminoethyl-sephadex column. the enzyme acted on the purine ribosides (except xanthosine) and on their 5'-phosphates. action on the latter was a good means for preparing ribose-5-phosphate. | 1968 | 5726308 |
| production of amylase by aspergillus foetidus on rice flour medium and characterization of the enzyme. | aspergillus foetidus atcc 10254 was selected from nine starch-utilizing microorganisms for its high amylolytic activity. this mould produced high levels of extracellular alpha-amylase in rice starch medium and degraded the available starch efficiently. optimal conditions for enzyme production on 2.0% rice medium included 28 degrees c, initial ph of 6.6, and supplementations with 0.02% nano2, 0.08% kh2po4, and 0.08% corn steep liquor. eleven-fold purification of the enzyme was obtained after ammo ... | 1984 | 6611331 |
| [comparative role of exo-1,4-beta-glucosidase and cellobiase in the enzymatic hydrolysis of cellulose]. | an inhibitory action of glucono-delta-lactone on individual components of cellulase complexes from trichoderma reesei, t. longibrachiatum, t. lignorum and aspergillus foetidus has been studied. it was shown that gluconolactone exerts an inhibiting effect on cellobiases only (the inhibition constants varied within the range of 0.03-0.1 mm) and does not influence the activities of endoglucanases, cellobiohydrolases and exoglucosidases of the complexes. this formed a basis for a new method for dete ... | 1981 | 6788099 |
| [enzymatic hydrolysis of cellulose. inactivation and stabilization of the enzymes of the cellulose complex]. | the thermal inactivation of the individual cellulolytic components (endoglucanase, ec 3.2.1.4; exoglucosidase, ec 3.2.1.74; cellobiase, ec 3.2.1.21) from the fungi trichoderma reesei, t. viride, t. lignorum an aspergillus foetidus has been studied without resolution of the cellulase complexes. the kinetics of the thermal inactivation follow the first order for cellobiase of asp. foetidus alone and show a more complex picture which is typical for a number of isoenzymes of different thermal stabil ... | 1982 | 6812652 |
| [effect of the carbon sources and metabolic inhibitors on endopolymethylgalacturonase secretion in aspergillus foetidus]. | the mycelium of aspergillus foetidus was grown for 48 h, washed off, and used to study the effect of various carbon sources on the synthesis and secretion of endopolymethylgalacturonase (endo-pmg). sucrose, glucose, glucose-6-phosphate, fructose, pectin and mannitol were shown to favour the enzyme secretion from the mycelium. the enzyme accumulation in the medium was inhibited when such inhibitors of metabolism as sodium azide and fluoride, 2,4-dinitrophenol and n,n'-dicyclohexyl carbodiimide we ... | 1982 | 7155005 |
| [hydrolysis of microcrystalline cellulose by multienzyme cellulase complexes of various origins]. | the kinetic regularities of glucose and cellobiose formation from microcrystalline cellulose (mcc) under the action of cellulase complexes from eight different sources were studied. by means of successive addition of selected components of the cellulase complexes (endoglucanase and cellobiase) to the reaction system the rate-limiting steps for multienzymatic hydrolysis of mcc were determined. it was shown that in most cases the rate-limiting step of glucose formation (via hydrolysis of the inter ... | 1980 | 7248366 |
| double-stranded rna mycoviruses in section nigri of the aspergillus genus. | double-stranded rna bands were detected electrophoretically in about 7% of natural isolates and in 13 of 51 collection strains belonging in section nigri of the genus aspergillus. the identity of these bands was proved by s1 nuclease and rnase treatment. most of the virus-containing natural isolates came from indonesia. electron microscopic examination of the strains revealed the presence of virus-like particles in the mycelia of the strains examined. all of the virus-like particles were isometr ... | 1994 | 8039055 |
| probiotics and manipulation of rumen development and function. | there is good evidence that the bacterial and fungal probiotics are effective in the manipulation of rumen development and function. the effects of adherent streptococcus bovis preparations were manifested by significant increase of adherent s. bovis bacteria and alpha amylase activity in the rumen wall of young ruminants. fungal probiotics based on saccharomyces cerevisiae and aspergillus oryzae are rapidly gaining acceptance as a means of improving productivity in adult ruminants. however, fun ... | 1993 | 8215880 |
| fungal biotransformation of organophosphines. | 1. the biotransformations of triphenyl phosphite and of triphenyl-, tri-n.-butyl-, diethylphenyl-, and ethylmethylphenyl-phosphines by the fungi mortierella isabellina atcc 42613, helminthosporium species nrrl 4671, and aspergillus foetidus atcc 10254, have been examined. 2. the triaryl and tri-n.-butyl substrates underwent oxidation at phosphorus in low yield, a process attributed largely to auto-oxidation under the experimental conditions used. 3. the alkyl aryl phosphines were enzymically oxi ... | 1993 | 8342299 |
| production of 1-kestose in transgenic yeast expressing a fructosyltransferase from aspergillus foetidus. | sucrose-inducible secretory sucrose:sucrose 1-fructosyltransferase (1-sst) from aspergillus foetidus has been purified and subjected to n-terminal amino acid sequence determination. the enzyme is extensively glycosylated, and the active form is probably represented by a dimer of identical subunits with an apparent molecular mass of 180 kda as judged from mobility in seminative acrylamide gels. the enzyme catalyzes fructosyl transfer from sucrose to sucrose producing glucose and 1-kestose. oligos ... | 1998 | 9495772 |
| use of hemicellulose hydrolysate for beta-glucosidase fermentation. | hydrolysis of cellulose by trichoderma cellulases often results in a mixture of glucose, cellobiose, and low-mol-wt cellodextrins. cellobiose is nonfermentable for most yeasts, and therefore it has to be hydrolyzed to glucose by beta-glucosidase prior to ethanol fermentation. in the present study, the beta-glucosidase production of one penicillium and three aspergillus strains, which were previously selected out of 24 strains, was investigated on steam pretreated willow. both steam-pretreated wi ... | 1998 | 9627384 |
| [enzymatic hydrolysis of willow treated with a steam burst without preliminary water extraction with a high concentration of substrate]. | a laboratory reactor equipped with a screw press was used for hydrolysis of steam-so2 exploded willow salix caprea by a composition of trichoderma reesei and aspergillus foetidus enzyme preparations at high substrate concentrations. optimal conditions providing the maximal volume of hydrolysis syrup with maximal sugar concentrations were determined. two different hydrolysis procedures were developed in order to exclude initial washing of steam-pretreated plant raw material by large volumes of wa ... | 2000 | 10779997 |
| cloning, developmental, and tissue-specific expression of sucrose:sucrose 1-fructosyl transferase from taraxacum officinale. fructan localization in roots. | sucrose:sucrose 1-fructosyl transferase (1-sst) is the key enzyme initiating fructan synthesis in asteraceae. using reverse transcriptase-pcr, we isolated the cdna for 1-sst from taraxacum officinale. the cdna-derived amino acid sequence showed very high homology to other asteracean 1-ssts (cichorium intybus 86%, cynara scolymus 82%, helianthus tuberosus 80%), but homology to 1-sst from allium cepa (46%) and aspergillus foetidus (18%) was much lower. fructan concentrations, 1-sst activities, 1-s ... | 2000 | 10806226 |
| [enzyme regeneration during hydrolysis of steam-pretreated willow and requirement for cellulase complex composition]. | in order to reduce the total enzyme consumption in high-solids static hydrolysis of nonwashed steam-exploded willow salix caprea by mixed cellulase of trichoderma reesei + aspergillus foetidus, two different approaches were proposed. in the first case, the enzyme activity adsorbed on residual solids after extended hydrolysis was used for hydrolysis of the newly added substrate. the initial mixing of fresh and hydrolyzed substrates was sufficient for the adsorbed enzyme redistribution and convers ... | 2000 | 10867945 |
| biosynthesis of nutraceutical iso-oligosaccharides by multiple forms of transferase produced by aspergillus foetidus. | isomalto-oligosaccharide and isofructo-oligosaccharide production was attempted using enzymes produced by aspergillus foetidus. four fractions having transferase enzyme activity were obtained from the fermentation broth of aspergillus foetidus by ammonium sulfate precipitation and deae-cellulose chromatography. the optimum temperature was 60 degrees c and ph stability in the range 4 to 6 for various fractions. the ph optima, heat sensitivity and kinetic parameters for the four fractions were how ... | 2000 | 10907244 |
| uptake of reactive textile dyes by aspergillus foetidus. | an isolated fungus, aspergillus foetidus was found to effectively decolorize media containing azo reactive dyes namely, drimarene dyes. the extent of color removal was greater than 95% within 48 h of growth of the fungus. the entire color was found to be strongly bioadsorbed to the rapidly settling fungal biomass pellets without undergoing significant biotransformation. our investigations reveal that the process of decolorization is concomitant with the exponential growth phase of the fungus and ... | 2000 | 10938413 |
| fungal mediated decolorization of media containing procion dyes. | an isolated fungus, aspergillus foetidus was found to effectively decolorize media containing azo reactive dyes, namely procion dyes, under aerobic conditions. the extent of colour removal was 90% within 48 h of growth of the fungus. the entire colour was found to be strongly bioadsorbed to the rapidly settling spherical fungal biomass pellets. our investigations reveal that the process of decolorization is concomitant with the exponential growth phase of the fungus and has an obligate requireme ... | 2001 | 11380192 |
| identification, classification and phylogeny of the aspergillus section nigri inferred from mitochondrial cytochrome b gene. | the partial mitochondrial cytochrome b gene from 32 strains of 12 species belonging to aspergillus section nigri was amplified by the polymerase chain reaction and sequenced directly. using 402 nucleotide characters, nucleotide-based and amino acid-based phylogenetic trees were inferred and the genetic divergence among the species was evaluated. based on analyses of the 402-bp nucleotide and 133-amino acid sequences, strains were divided into 11 dna types and five amino acid types. aspergillus n ... | 2001 | 11425482 |
| purification, characterization, gene cloning and preliminary x-ray data of the exo-inulinase from aspergillus awamori. | extracellular exo-inulinase has been isolated from a solid-phase culture of the filamentous fungus aspergillus awamori var. 2250. the apparent molecular mass of the monomer enzyme was 69 +/- kda, with a pi of 4.4 and a ph optimum of 4.5. the enzyme hydrolysed the beta-(2-->1)-fructan (inulin) and beta-(2-->6)-fructan (levan) via exo-cleavage, releasing fructose. the values for the michaelis constants k(m) and v(max) in the hydrolysis of inulin were 0.003 +/- 0.0001 mm and 175 +/- 5 micromol.min( ... | 2002 | 11829749 |
| biosynthesis of tannase and gallic acid from tannin rich substrates by rhizopus oryzae and aspergillus foetidus. | modified solid-state fermentation (mssf) of tannin-rich substrates for production of tannase and gallic acid was carried out using two fungal cultures, rhizopus oryzae (ro iit rb-13, nrrl 21498) and aspergillus foetidus (gmrb013 mtcc 3557). the tannin rich substrates included powdered fruits of terminalia chebula and caesalpinia digyna pod cover powder. the different environmental parameters for the maximum production of tannase and gallic acid were optimized through media engineering. the highe ... | 2004 | 14768027 |
| optimization of a biological process for treating potato chips industry wastewater using a mixed culture of aspergillus foetidus and aspergillus niger. | potato chips industry wastewater was collected and analyzed for biochemical oxygen demand (bod), chemical oxygen demand (cod), total suspended solids (tss) and total carbohydrates. two aspergillus species, a. foetidus and a. niger, were evaluated for their ability to grow and produce biomass and reduce the organic load of the wastewater. a. foetidus mtcc 508 and a. niger itcc 2012 were able to reduce cod by about 60% and produce biomass 2.4 and 2.85 gl(-1), respectively. co-inoculation of both a ... | 2004 | 15081479 |
| microbial transformation of tannin-rich substrate to gallic acid through co-culture method. | modified solid-state fermentation (mssf) of tannin-rich substrate yielding tannase and gallic acid was carried out using a co-culture of the filamentous fungi, rhizopus oryzae (ro iit rb-13, nrrl 21498) and aspergillus foetidus (gmrb013 mtcc 3557). powdered fruits of terminalia chebula and powdered pod cover of caesalpinia digyna was used in the process and the different process parameters for maximum production of tannase and gallic acid by co-culture method were optimized through media enginee ... | 2005 | 15627566 |
| aspergillus vadensis, a new species of the group of black aspergilli. | a strain from the group of black aspergilli was analysed in detail to determine the species to which it belongs. a detailed analysis of morphology, rflp patterns and metabolite profiles was carried out. in addition, a phylogenetic tree was constructed for the black aspergilli using the its and the beta-tubulin sequences of the individual strains. the new species differs by its poor growth on glycerol and galacturonate and its unique extrolite profile consisting of aurasperone b, nigragillin, asp ... | 2005 | 15803385 |
| xylanase production by fungal strains on spent sulphite liquor. | xylanase production by seven fungal strains was investigated using concentrated spent sulphite liquor (sslc), xylan and d: -xylose as carbon substrates. an sslc-based medium induced xylanase production at varying levels in all of these strains, with aspergillus oryzae nrrl 3485 and aspergillus phoenicis atcc 13157 yielding activities of 164 and 146 u ml(-1), respectively; these values were higher than those obtained on xylan or d: -xylose with the same fungal strains. the highest xylanase activi ... | 2005 | 15944854 |
| strain improvement for tannase production from co-culture of aspergillus foetidus and rhizopus oryzae. | spores from the co-culture of aspergillus foetidus and rhizopus oryzae were subjected to uv, heat and ntg (3-nitro,5-methylguanidine) mutagenesis. a few colonies were screened from the selected media for tannase study. amongst all, the best mutant isolated from the heat treatment (60 degrees c for 60 min) was scpr 337. the maximum yield of gallic acid and tannase in case of mutant strain was 95.2% and 53.6 u/ml with an incubation period of 30 h as compared to wild strain where the incubation per ... | 2006 | 15963714 |
| improvement of the quality of whole wheat bread by supplementation of xylanase from aspergillus foetidus. | the possible use of xylanase from aspergillus foetidus mtcc 4898 as a bread improver was tested in whole wheat bread. the partially purified xylanase was used as an additive at 12 u/g during mixing of wheat flour. the effects of xylanase addition on the fermentation stage and the final bread quality were analyzed. remarkable decrease (11%) in water absorption and increase in dough rising (28.5%) were noticed. final moisture content of the bread was higher (40.5%) than control (32.3%). improvemen ... | 2006 | 16307877 |
| baked-bean waste: a potential substrate for producing fungal amylases. | baked-bean waste was found to be a favorable substrate for amylase production by aspergillus foetidus nrrl 337. under optimum conditions, the yields of alpha-amylase (ec 3.2.1.1) and glucoamylase (ec 3.2.1.3) were 47 and 226 u, respectively, per ml of the waste fermented. | 1977 | 16345247 |
| kinetic and equilibrium studies on the biosorption of reactive black 5 dye by aspergillus foetidus. | an isolated fungus, aspergillus foetidus had the ability to decolourize growth unsupportive medium containing 100 mg l(-1) of reactive black 5 (rb5) dye with >99% efficiency at acidic ph (2-3). pre-treatment of fungal biomass by autoclaving or exposure to 0.1m sodium hydroxide facilitated more efficient uptake of dye as compared to untreated fungal biomass. pre-equilibrium biosorption of rb5 dye onto fungus under different temperatures followed pseudo-second-order kinetic model with high degree ... | 2008 | 17251011 |
| effect of cultivating conditions on alpha-galactosidase production by a novel aspergillus foetidus zu-g1 strain in solid-state fermentation. | the work is intended to achieve optimum culture conditions of alpha-galactosidase production by a mutant strain aspergillus foetidus zu-g1 in solid-state fermentation (ssf). certain fermentation parameters involving moisture content, incubation temperature, cultivation period of seed, inoculum volume, initial ph value, layers of pledget, load size of medium and period of cultivation were investigated separately. the optimal cultivating conditions of alpha-galactosidase production in ssf were 60% ... | 2007 | 17542067 |
| response surface methodology for optimizing the fermentation medium of alpha-galactosidase in solid-state fermentation. | alpha-galactosidase is applied in food and feed industries for hydrolysing raffinose series oligosaccharides (ro) that are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. the objective of the current work was to develop an optimal culture medium for the production of alpha-galactosidase in solid-state fermentation (ssf) by a mutant strain aspergillus foetidus. | 2007 | 17651220 |
| optimization of the fermentation medium for alpha-galactosidase production from aspergillus foetidus zu-g1 using response surface methodology. | the optimization of fermentation medium for alpha-galactosidase production by aspergillus foetidus zu-g1 was investigated in shaker flask fermentation. a one-factor-at-a-time experiment was used to screen the preferable nutriment (carbon sources, nitrogen sources, and essential elements) for alpha-galactosidase production. a fractional factorial design was used to screen the main 5 factors, soybean meal, wheat bran, kh2po4, feso4 x 7 h2o, and the medium initial ph, that affected the production o ... | 2007 | 17995779 |
| use of hemicellulose hydrolysate for beta-glucosidase fermentation. | hydrolysis of cellulose by trichoderma cellulases often results in a mixture of glucose, cellobiose, and low-mol-wt cellodextrins. cellobiose is nonfermentable for most yeasts, and therefore it has to be hydrolyzed to glucose by beta-glucosidase prior to ethanol fermentation. in the present study, the beta-glucosidase production of one penicillium and three aspergillus strains, which were previously selected out of 24 strains, was investigated on steam pretreated willow. both steam-pretreated wi ... | 1998 | 18575992 |
| optimization of xylanase production from aspergillus foetidus mtcc 4898 by solid state fermentation using statistical methods. | | 2010 | 20339224 |
| application of response surface analysis for biodegradation of azo reactive textile dye using aspergillus foetidus. | this paper reports the application of experimental design methodology for the optimization of decolourization of azo reactive textile dye remazol red rr and reduction of chemical oxygen demand (cod) using fungal isolate aspergillus foetidus. response surface methodology (rsm), involving central composite design matrix in three most important input variables; temperature, ph and initial dye concentration was employed. a total of 20 experiments were conducted in the study towards the construction ... | 2011 | 21780149 |
| Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics. | The selective production of xylooligosaccharides (XOS) was carried out using partially purified xylanase from Aspergillus foetidus MTCC 4898. Corncob xylan was extracted using a mild alkali treatment which yielded 178.73±5.8gofxylan/kg of corncobs. Partially purified ß-xylosidase free xylanase was found efficient in releasing xylooligosaccharides from corncob xylan. Maximum yield of xylooligosaccharides was 6.73±0.23mg/ml after 8h of reaction time using 20U of xylanase at 45°C. Purification of X ... | 2011 | 22100233 |
| Genetic re-identification and antifungal susceptibility testing of Aspergillus section Nigri strains of the BCCM/IHEM collection. | Black Aspergilli are widely distributed in the environment and are frequently reported as causative agents of different types of mycoses. Many taxonomical revisions have been made, and presently 19 different species are accepted. In this study we (re-) identified 123 strains of the Aspergillus niger group of the BCCM/IHEM collection to check for the presence of species other than A. niger in both environmental and clinical samples. The susceptibility for antifungal drugs was compared between A. ... | 2011 | 21929560 |
| optimization of parameters for enhanced oil recovery from enzyme treated wild apricot kernels. | present investigation was undertaken with the overall objective of optimizing the enzymatic parameters i.e. moisture content during hydrolysis, enzyme concentration, enzyme ratio and incubation period on wild apricot kernel processing for better oil extractability and increased oil recovery. response surface methodology was adopted in the experimental design. a central composite rotatable design of four variables at five levels was chosen. the parameters and their range for the experiments were ... | 2011 | 23904657 |
| deep sequencing analysis reveals the mycoviral diversity of the virome of an avirulent isolate of rhizoctonia solani ag-2-2 iv. | rhizoctonia solani represents an important plant pathogenic basidiomycota species complex and the host of many different mycoviruses, as indicated by frequent detection of dsrna elements in natural populations of the fungus. to date, eight different mycoviruses have been characterized in rhizoctonia and some of them have been reported to modulate its virulence. dsrna extracts of the avirulent r. solani isolate dc17 (ag2-2-iv) displayed a diverse pattern, indicating multiple infections with mycov ... | 2016 | 27814394 |
| sequence determination of a quadripartite dsrna virus isolated from aspergillus foetidus. | virus infection of aspergillus foetidus was documented over 40 years ago and was one of the first mycovirus infections described in a filamentous fungus. the virus, named aspergillus foetidus virus (afv), contains at least two types of icosahedral particles, called afv-fast (-f) and afv-slow (-s) virions, based on their relative electrophoretic mobilities. here, we report the complete nucleotide sequence of the afv-f genome isolated from virions purified from the prototype isolate of the fungus. ... | 2013 | 22760661 |
| taxonomic re-evaluation of black koji molds. | black koji molds including its albino mutant, the white koji mold, have been widely used for making the distilled spirit shochu in northeast asia because they produce citric acid which prevents undesirable contamination from bacteria. since inui reported aspergillus luchuensis from black koji in okinawa in 1901, many fungal names associated with black koji molds were reported. however, some species are similar and differentiation between species is difficult. fungal taxonomists tried to arrange ... | 2014 | 24281756 |
| analyses of black aspergillus species of peanut and maize for ochratoxins and fumonisins. | the genus aspergillus section nigri, or the black aspergilli, represents genetically closely related species that produce the mycotoxins, ochratoxins and the fumonisins. fumonisin b1 (fb1) is of an added concern because it is also a virulence factor for maize. our preliminary data indicated that black aspergilli could develop asymptomatic infections with maize and peanuts plants. symptomless infections are potential problems, because under favorable conditions, there is a potential for accumulat ... | 2014 | 24780336 |
| positive and negative aspects of green coffee consumption - antioxidant activity versus mycotoxins. | the quality of coffee depends not only on the contents of healthy compounds but also on its contamination with microorganisms that can produce mycotoxins during development, harvesting, preparation, transport and storage. | 2017 | 28195330 |
| stereoselective reduction of prochiral ketones by plant and microbial biocatalysts. | chiral alcohols are the key chiral building blocks to many enantiomerically pure pharmaceuticals. the biocatalytic approach in asymmetric reduction of corresponding prochiral ketones to the preparation of these optically pure substances is one of the most promising routes. the stereoselective reduction of different kinds of prochiral ketones catalyzed by various plants and microorganisms was studied in this work. benzyl acetoacetate, methyl 3-oxopentanoate, ethyl 3-oxopentanoate, and ethyl butyr ... | 2017 | 27168684 |
| comparative study of two purified inulinases from thermophile thielavia terrestris nrrl 8126 and mesophile aspergillus foetidus nrrl 337 grown on cichorium intybus l. | thirty fungal species grown on cichorium intybus l. root extract as a sole carbon source, were screened for the production of exo-inulinase activities. the thermophile thielavia terrestris nrrl 8126 and mesophile aspergillus foetidus nrrl 337 gave the highest production levels of inulinases i & ii at 50 and 24 ºc respectively. yeast extract and peptone were the best nitrogen sources for highest production of inulinases i & ii at five and seven days of incubation respectively. the two inulinases ... | 2011 | 24031675 |
| kinetic and thermodynamic studies of a novel acid protease from aspergillus foetidus. | the kinetics of a thermostable extracellular acid protease produced by an aspergillus foetidus strain was investigated at different ph, temperatures and substrate concentrations. the enzyme exhibited maximal activity at ph 5.0 and 55°c, and its irreversible deactivation was well described by first-order kinetics. when temperature was raised from 55 to 70°c, the deactivation rate constant increased from 0.018 to 5.06h(-1), while the half-life decreased from 37.6 to 0.13h. the results of activity ... | 2015 | 26210038 |
| design, synthesis, and biological evaluation of vanillin hydroxamic acid derivatives as novel peptide deformylase inhibitors. | infectious disease is increasingly hampering human health, which challenge the discovery of new antibacterial target. peptide deformylase (pdf), a metalloenzyme responsible for catalyzing the remove of the n-formyl group from nascent proteins, was considered as an important target in antibacterial drug discovery. | 2017 | 28606047 |
| multiple virus infection in a single strain of fusarium poae shown by deep sequencing. | many bands were detected on an electrophoretic profile of double-stranded (ds) rna preparation from a single strain of fusarium poae isolated from wheat. when the purified dsrna sample was deep-sequenced by a next-generation sequencer, sixteen virus-like assembled contigs with predicted amino acid sequences showing homologies to respective viral rna-dependent rna polymerases (rdrps) were found by blast analysis. fourteen out of sixteen sequences showed homologies to rdrps of known mycoviruses, t ... | 2016 | 27550368 |
| a novel dsrna element isolated from the aspergillus foetidus mycovirus complex. | aspergillus foetidus virus (afv) contains at least two icosahedral particle types named afv-fast (-f) and afv-slow (-s), based on relative electrophoretic mobility. afv-f is a quadripartite double-stranded rna (dsrna) virus, and afv-s contains afv-s1, which is a member of the genus victorivirus in the family totiviridae, and afv-s2, which may be a satellite rna or satellite virus and is described here. analysis of the complete afv-s2 nucleotide sequence reveals it to be significantly similar to ... | 2013 | 23827976 |
| bioremediation potential of arsenic by non-enzymatically biofabricated silver nanoparticles adhered to the mesoporous carbonized fungal cell surface of aspergillus foetidus mtcc8876. | our study aiming for the diminution of arsenic concentration in arsenic contaminated aqua environment by using novel adsorbents based on bio-fabricated silver nanoparticles on a fungal cell surface. bioreduction of silver ion was done in a non-enzymatic manner in presence of the biomass of fungus aspergillus foetidus mtcc8876. aspergillus spp. cells were harvested following the bioreduction and made into carbon by carbonization that led to pore formation (pore width of 2.35 nm) on the cell surfa ... | 2017 | 28710960 |
| production, purification and characterization of an aspartic protease from aspergillus foetidus. | an acidic thermostable protease was extracellularly produced either in shake flask or in stirred tank bioreactor by an aspergillus foetidus strain isolated from the brazilian savanna soil using different nitrogen sources. its maximum activity (63.7 u ml-1) was obtained in a medium containing 2% (w/v) peptone. a cultivation carried out in a 5.0 l stirred-tank bioreactor provided a maximum protease activity 9% lower than that observed in erlenmeyer flasks, which was obtained after a significantly ... | 2017 | 28359876 |
| production of α-galactosidase from aspergillus foetidus mtcc 6322 by solid state fermentation and its application in soymilk hydrolysis. | the production of α-galactosidase from the wild fungal strain aspergillus foetidus mtcc 6322 using solid state fermentation (ssf), its characterization, and its efficacy in the hydrolysis of soymilk using response surface methodology were studied. the optimum conditions for production of α-galactosidase by ssf were: wheat bran (10 g), moisture content (64%), inoculum volume (1.0 ml; 6 x 10(7) spores/ml) with a yield of 4.1 x 10(3) units per gram dry substrate (u/gds) at 96 h. the enzyme showed o ... | 2016 | 26891555 |
| optimization, purification and characterization of pectinases from pectinolytic strain, aspergillus foetidus mtcc 10559. | a strain (mpuat-2), isolated from coconut hull and identified as aspergillus foetidus mtcc 10559, was used for pectinase production. optimum pectinase production was obtained at ph 8.0 and temperature 35 degrees c under static conditions in submerged fermentation after 5 days of incubation. orange peel, a byproduct of fruit industry, was used as a sole carbon source (3% w/v) to produce high pectinase, thus making the process cost effective. the culture filtrate was analyzed for pectin methyl est ... | 2015 | 25895274 |
| sequence determination of a satellite rna isolated from aspergillus foetidus. | aspergillus foetidus virus (afv) has at least two distinct particle types, designated as afv-fast (f) and afv-slow (s). afv-s includes afv-s1, a victorivirus; afv-s2, an unclassified satellite rna; and afv-s3, a previously uncharacterized dsrna element. here, we describe the complete sequence of afv-s3, which is a short non-coding rna with no known homologs. afv-s3 is predicted to form an extended secondary structure, shares a 5' terminus with afv-s2, and is a satellite rna possibly dependent on ... | 2015 | 25613164 |
| cadmium-induced oxidative stress tolerance in cadmium resistant aspergillus foetidus: its possible role in cadmium bioremediation. | toxic effects of cadmium (cd) were examined on a cadmium-resistant strain of aspergillus foetidus isolated from wastewater. the cd removal potential was analyzed. the results indicated that the strain could tolerate up to 25 mm and 63 mm cd in liquid and solid czapek-dox media, respectively. it efficiently removed cd from liquid growth media and industrial wastewater by mycelial biosorption. the strain produced oxalic acid for the purpose of cd bioleaching as confirmed by the presence of cadmium ... | 2014 | 24836877 |
| gastrodin production from p-2-hydroxybenzyl alcohol through biotransformation by cultured cells of aspergillus foetidus and penicillium cyclopium. | the objective of this work was to take advantage of the resting cells of suitable fungus as an in vitro model to prepare gastrodin from p-2-hydroxybenzyl alcohol (hba), which mainly exists in the metabolites of the plant gastrodia elata blume. the one-step biotransformation of hba into gastrodin was examined with the filamentous fungi cells of aspergillus foetidus and penicillium cyclopium as 3.4513 in this study. the fundamental conditions of biotransformation were screened and compared for bot ... | 2013 | 23483410 |
| the complete nucleotide sequence of a totivirus from aspergillus foetidus. | virus infection of aspergillus foetidus was first described in the 1970s, and the purified virus, named aspergillus foetidus virus (afv), contains at least two types of icosahedral particles, called afv-fast (-f) and afv-slow (-s) virions, based on their relative electrophoretic mobilities. afv-s consists of a mixture of two viruses, the larger of which, called afv-s1, is a dsrna-containing member of the genus victorivirus in the family totiviridae, and its complete nucleotide sequence is descri ... | 2013 | 22729614 |