| fungal exudates. | the exudates or liquid droplets on various structures of a number of fungi were examined. the droplets were enveloped in membranous material and were associated with actively growing mycelia, including fruiting structures. osmium tetroxide vapour-fixed droplets of claviceps purpurea, myrothecium roridum, sclerotinia sclerotiorum, sclerotium rolfsii, and thanathephorus cucumeris did not dry to a powder but remained intact as spheres when freeze-dried. fractured spheres, examined with the scanning ... | 1978 | 728849 |
| keratinolytic and keratinophilic fungi in the soils of papua new guinea. | a study was made of soil samples collected during an expedition to the highlands of papua new guinea. fungi were isolated from the samples by the method of hair baiting (to-ka-va). of the 33 species isolated, about half showed keratinolytic activity. such activity is previously unreported for mucor hiemalis f. hiemalis, myrothecium roridum, paecilomyces carneus, p. marquandii, penicillium brevicompactum, rhinocladiella mansonii and verticillium lecanii. the species most active keratinolytically ... | 1991 | 1723786 |
| isolation and purification of a hemorrhagic factor (wortmannin) from fusarium oxysporum (n17b). | an isolate of fusarium oxysporum schlecht, emend. synd. et hans. n17b isolated from a grassy area in lakselv, norway (arctic region) produced a toxin in culture when grown on rice in the laboratory. this new toxin, which was given the trivial name of h-1 (indicating hemorrhagic factor), caused toxic effects in rats, including food refusal, weight loss, hemorrhage in the stomach, intestines, heart, and thymus, and finally death. the uv spectrum of h-1 showed 210, 254, and 292 nm as absorption max ... | 1988 | 3389818 |
| 7 alpha-hydroxytrichodermol, a new trichothecene from myrothecium roridum. | five plant-pathogenic isolates of myrothecium roridum from florida produced only simple trichothecenes rather than the usual macrocyclic trichothecenes. the major metabolite was 7 alpha-hydroxytrichodermol. | 1985 | 4091555 |
| [isolation of 2'-dehydroverrucarin a as metabolite of myrothecium roridum tode ex fr.,species type by fries. verrucarin and roridin, 13]. | | 1966 | 6010054 |
| the isolation and identification of trichothecene metabolites from a plant pathogenic strain of myrothecium roridum. | | 1984 | 6541681 |
| characterization of the gene cluster for biosynthesis of macrocyclic trichothecenes in myrothecium roridum. | macrocyclic trichothecenes are toxic sesquiterpenoids that are produced by certain fungi and plants. the unique structural features of macrocyclic trichothecenes result in increased toxicity relative to other trichothecene structural types. here we report the sequences and relative locations of the mrtri5, mrtri6, and mrtri4 genes in the biosynthetic pathway for macrocyclic trichothecenes in myrothecium roridum. the deduced sequences of the products of mrtri5 and mrtri4 display overall identitie ... | 1998 | 9529523 |
| characterization of a transcriptional activator controlling trichothecene toxin biosynthesis. | trichothecene biosynthetic pathway genes are localized within a gene cluster in fusarium sporotrichioides and require the zinc-finger containing protein, tri6, for expression. we show here that tri6 is able to bind within the promoter regions of nine different pathway genes and that tri6 binding is involved in pathway gene activation. tri6 binding occurs at three distinct sites in the tri5 promoter, all of which contain the sequence tnaggcct. dna fragments from the promoter regions of six other ... | 1999 | 10361036 |
| a new macrocyclic trichothecene, 12,13-deoxyroridin e, produced by the marine-derived fungus myrothecium roridum collected in palau. | a new macrocyclic trichothecene, 12,13-deoxyroridin e (1), and three known compounds, roridin e (2), verrucarin a (3), and verrucarin j (4), were obtained as cytotoxic components from the marine-derived fungus myrothecium roridum, isolated in palau. 12,13-deoxyroridin e is the second example of a macrocyclic trichothecene possessing a double bond at c-12-c-13 and was about 80-fold less cytotoxic than roridin e, the epoxide variant. | 2001 | 11277768 |
| 14'-hydroxymytoxin b and 16-hydroxyroridin e, two new cytotoxic trichothecenes from myrothecium roridum. | two new trichothecenes, 14'-hydroxymytoxin b (1) and 16-hydroxyroridin e (3), were isolated from a fermentation extract of myrothecium roridum. the structures of 1 and 3 were determined by spectral data interpretation. both compounds showed potent cytotoxic activity against primary soft-tissue sarcoma cells. | 2002 | 12027756 |
| antibiotic properties of mycelium and metabolites of myrothecium roridum tode. | | 1961 | 14479074 |
| chitosan (biochikol 020 pc) in the control of some ornamental foliage diseases. | chitosan, (biochikol 020 pc) a potential elicitor of plant defence and also an active inhibitor of fungal growth was used in experiments. the compound was used at concentrations 0.01 to 0.2% as a plant spray for rose protection against sphaerotheca pannosa var. rosae, peronospora sparsa and diplocarpon rosae, oidium chrysanthemi and puccinia horiana on chrysanthemum, against melampsora epitea on willow. myrothecium roridum on dieffenbachia and against lophodermium spp on scots pine. effectivenes ... | 2004 | 15756862 |
| synthesis and antifungal potential of co(ii) complexes of 1-(2'-hydroxyphenyl) ethylideneanilines. | synthesis of co(ii) complexes from 1-(2'-hydroxyphenyl)-ethylideneaniline and its n-phenyl derivatives has been described and their structures have been established on the basis of elemental analysis and ir studies. the complexes have been screened for their antifungal potential against alternaria alternata, fusarium oxysporum and myrothecium roridum by spore germination inhibition method and the results are discussed. | 2006 | 16256249 |
| absolute configuration of new cytotoxic and other bioactive trichothecene macrolides. | three new cytotoxic 10,13-cyclotrichothecane-derived macrolides, myrothecines a-c (1-3), were characterized from the extracts of two myrothecium roridum strains, ifb-e009 and ifb-e012, isolated as endophytic fungi found on the traditional chinese medicinal plants trachelospermum jasminoides and artemisia annua, respectively. the absolute configuration of myrothecines a-c was elucidated by a combination of spectral techniques (uv, ir, ms, circular dichroism (cd), (1)h and (13)c nmr, dept, (1)h-(1 ... | 2006 | 16673428 |
| isolation and characterization of two verrucarins from myrothecium roridum. | in the course of a screening for compounds inhibiting the growth of two human breast cancer cells lines, two highly cytotoxic compounds were isolated from fermentations of myrothecium roridum. the elucidation of their structures revealed that they are macrocyclic trichothecenes of the verrucarin type, 16-hydroxyverrucarin a (1), reported here as a natural product for the first time, and verrucarin x (2), a new compound. both 1 and 2 exhibit moderate antifungal activity and pronounced cytotoxic a ... | 2006 | 16869484 |
| four new macrocyclic trichothecenes from two strains of marine-derived fungi of the genus myrothecium. | three new macrocyclic trichothecenes, named 12'-hydroxyroridin e (1), roridin q (2), and 2',3'deoxyroritoxin d (3), were isolated from the marine-derived fungus myrothecium roridum tuf 98f42, and a new macrocyclic trichothecene, named roridin r (4), was isolated from myrothecium sp. tuf 02f6 together with roridins a and h and isororidin e. the structures of new compounds were determined on the basis of their spectral data. compound 2 possessed a unique ether moiety at the 13' position of 1. comp ... | 2006 | 17080680 |
| myrothecium roridum tri4 encodes a multifunctional oxygenase required for three oxygenation steps. | the biosyntheses of both macrocyclic trichothecenes in myrothecium roridum and simple trichothecenes in fusarium species begin with the cyclization of farnesyl pyrophosphate to form the sesquiterpene hydrocarbon trichodiene. a previous study showed that myrothecium has a cluster of 3 genes that are homologous with fusarium trichothecene genes: tri4, a p450 oxygenase; tri5, the sesquiterpene cyclase; and tri6, a zinc-finger regulatory gene. fusarium graminearum tri4 (fgtri4) and m. roridum mrtri4 ... | 2007 | 17668015 |
| a new cytotoxic trichothecene macrolide from the endophyte myrothecium roridum. | a new cytotoxic roridin-type trichothecene macrolide named roritoxin e(1) was characterized from the solid culture of myrothecium roridum ifb-e091 (residing originally inside artemisia annua root), together with the known compounds lumichrome (2), (22 e,24 s)-cerevisterol (3), and (22 e,24 r)-6 beta-methoxy-ergosta-7,22-diene-3 beta,5 alpha-diol (4). the structure of the new macrolide (1) was elucidated by a combination of spectroscopic (uv, ir, ms, and 1d and 2d nmr) analyses. compound 1 was de ... | 2010 | 20195960 |
| biological delignification of paddy straw and parthenium sp. using a novel micromycete myrothecium roridum lg7 for enhanced saccharification. | a new lignolytic micromycete fungus myrothecium roridum lg7 was isolated and selected for biological delignification of agro residue-paddy straw and herbaceous weed parthenium sp. physical and chemical modifications in the biomass following pretreatment with m. roridum lg7 for 7 days in term of structural modification and lignin removal, changes in lignin skeleton, and alteration of cellulose crystallinity was observed through sem-edxa, ftir and xrd analysis, respectively. colonization of the fu ... | 2012 | 23313177 |