| recessive resistance genes against potyviruses are localized in colinear genomic regions of the tomato ( lycopersicon spp.) and pepper ( capsicum spp.) genomes. | resistance against both potato virus y (pvy) and tobacco etch virus (tev) was identified in the wild tomato relative lycopersicon hirsutum pi247087. analysis of the segregation ratio in f(2)/f(3) and bc(1) interspecific progenies indicated that a single recessive gene, or two very tightly linked recessive loci, are involved in resistance to both potyviruses. this locus was named pot-1. using amplified fragment length polymorphism markers and a set of l. hirsutum introgression lines, pot-1 was ma ... | 2002 | 12582910 |
| proteomic analysis of resistance mediated by rcm 2.0 and rcm 5.1, two loci controlling resistance to bacterial canker of tomato. | two quantitative trait loci from lycopersicon hirsutum, rcm 2.0 and rcm 5.1, control resistance to clavibacter michiganensis subsp. michiganensis, the causal agent of bacterial canker of tomato. lines containing rcm 2.0 and rcm 5.1 and a susceptible control line were compared at 72 and 144 h postinoculation, using 2-dimensional gel electrophoresis to identify proteins regulated in response to c. michiganensis subsp. michiganensis infection. a total of 47 proteins were subjected to tandem mass sp ... | 2004 | 15384492 |
| expression of stress-response proteins upon whitefly-mediated inoculation of tomato yellow leaf curl virus in susceptible and resistant tomato plants. | to better understand the nature of resistance of tomato to the whitefly (bemisia tabaci, b biotype)-transmitted tomato yellow leaf curl virus (tylcv), whiteflies and tylcv were considered as particular cases of biotic stresses and virus resistance as a particular case of successful response to these stresses. two inbred tomato lines issued from the same breeding program that used solanum habrochaites as a tylcv resistance source, one susceptible and the other resistant, were used to compare the ... | 2007 | 17977149 |
| the am gene controlling resistance to alfalfa mosaic virus in tomato is located in the cluster of dominant resistance genes on chromosome 6. | abstract the dominant gene am from lycopersicon hirsutum f. sp. glabratum pi134417 confers resistance to most strains of alfalfa mosaic virus, including the recently identified necrotic strains. the phenotypic response includes a lack of symptom development following mechanical inoculation of leaves. to study the resistance mechanism controlled by am, biological (back-inoculation to susceptible hosts), serological (double-antibody sandwich, enzyme-linked immunosorbent assay), and molecular (reve ... | 2004 | 18944110 |
| a novel pathway for sesquiterpene biosynthesis from z,z-farnesyl pyrophosphate in the wild tomato solanum habrochaites. | in the wild tomato solanum habrochaites, the sst2 locus on chromosome 8 is responsible for the biosynthesis of several class ii sesquiterpene olefins by glandular trichomes. analysis of a trichome-specific est collection from s. habrochaites revealed two candidate genes for the synthesis of sst2-associated sesquiterpenes. zfps encodes a protein with homology to z-isoprenyl pyrophosphate synthases and sbs (for santalene and bergamotene synthase) encodes a terpene synthase with homology to kaurene ... | 2009 | 19155349 |
| silencing of a single gene in tomato plants resistant to tomato yellow leaf curl virus renders them susceptible to the virus. | a reverse-genetics approach was applied to identify genes involved in tomato yellow leaf curl virus (tylcv) resistance, taking advantage of two tomato inbred lines from the same breeding program-one susceptible (s), one resistant (r-that used solanum habrochaites as the source of resistance. cdna libraries from inoculated and non-inoculated r and s plants were compared, postulating that genes preferentially expressed in the r line may be part of the network sustaining resistance to tylcv. furthe ... | 2009 | 19533378 |
| isolation and functional characterization of lycopene beta-cyclase (cyc-b) promoter from solanum habrochaites. | carotenoids are a group of c40 isoprenoid molecules that play diverse biological and ecological roles in plants. tomato is an important vegetable in human diet and provides the vitamin a precursor beta-carotene. genes encoding enzymes involved in carotenoid biosynthetic pathway have been cloned. however, regulation of genes involved in carotenoid biosynthetic pathway and accumulation of specific carotenoid in chromoplasts are not well understood. one of the approaches to understand regulation of ... | 2010 | 20380705 |
| divergent regulation of terpenoid metabolism in the trichomes of wild and cultivated tomato species. | the diversification of chemical production in glandular trichomes is important in the development of resistance against pathogens and pests in two species of tomato. we have used genetic and genomic approaches to uncover some of the biochemical and molecular mechanisms that underlie the divergence in trichome metabolism between the wild species solanum habrochaites la1777 and its cultivated relative, solanum lycopersicum. la1777 produces high amounts of insecticidal sesquiterpene carboxylic acid ... | 2009 | 18997116 |
| enzymatic functions of wild tomato methylketone synthases 1 and 2. | the trichomes of the wild tomato species solanum habrochaites subsp. glabratum synthesize and store high levels of methylketones, primarily 2-tridecanone and 2-undecanone, that protect the plants against various herbivorous insects. previously, we identified cdnas encoding two proteins necessary for methylketone biosynthesis, designated methylketone synthase 1 (shmks1) and shmks2. here, we report the isolation of genomic sequences encoding shmks1 and shmks2 as well as the homologous genes from t ... | 2010 | 20605911 |
| multiple biochemical and morphological factors underlie the production of methylketones in tomato trichomes. | genetic analysis of interspecific populations derived from crosses between the wild tomato species solanum habrochaites f. sp. glabratum, which synthesizes and accumulates insecticidal methylketones (mk), mostly 2-undecanone and 2-tridecanone, in glandular trichomes, and cultivated tomato (solanum lycopersicum), which does not, demonstrated that several genetic loci contribute to mk metabolism in the wild species. a strong correlation was found between the shape of the glandular trichomes and th ... | 2009 | 19801397 |
| tomato breeding lines resistant and tolerant to tomato yellow leaf curl virus issued from lycopersicon hirsutum. | abstract two tomato yellow leaf curl virus (tylcv)-resistant plants from accessions la1777 and la386 of the wild tomato species lycopersicon hirsutum have been crossed. the resulting resistant f1 plants were crossed with the domesticated tomato l. esculentum, and a series of selfing was performed. at each generation, individuals were selected for resistance (no symptoms and undetectable viral dna) and tolerance (no symptoms but with detectable viral dna) following controlled massive and repeated ... | 1998 | 18944868 |
| two loci from lycopersicon hirsutum la407 confer resistance to strains of clavibacter michiganensis subsp. michiganensis. | abstract we used molecular markers to identify quantitative trait loci (qtl) that contribute to resistance to bacterial canker of tomato caused by clavibacter michiganensis subsp. michiganensis. resistance was first identified as a marker-trait association in an inbred backcross (ibc) population derived from crossing lycopersicon hirsutum accession (la407) with l. esculentum. single-marker qtl analysis suggested that at least two loci originating from l. hirsutum la407, rcm 2.0 on chromosome 2 a ... | 2002 | 18943024 |
| novel arthropod repellent, bioud, is an efficacious alternative to deet. | for >50 yr, n,n-diethyl-3-methylbenzamide (deet) has been the standard for arthropod repellents and has been an important tool to protect people from disease agents carried by ticks, mosquitoes, and other arthropods. however, some people avoid using deet because of concerns about adverse health effects. in 2007, a new repellent, bioud, with the active ingredient 7.75% 2-undecanone, originally derived from wild tomato (lycopersicon hirsutum dunal f. glabratum c. h. müll) plants, was registered by ... | 2008 | 18826032 |
| response of tomato and its wild relatives in the genus solanum to cucumber mosaic virus and satellite rna combinations. | the differential response of 29 genotypes of tomato and wild tomato relatives (solanum section lycopersicon species) to cucumber mosaic virus strain fny (cmv-fny), alone or in combination with three different satellite rna (satrna) variants, allowed the identification of four disease phenotype patterns, each including plants that developed very severe symptoms (leaf malformations, top stunting and lethal necrosis) and plants that remained asymptomatic. no resistance or tolerance to cmv-fny was o ... | 2007 | 17947544 |
| 2-tridecanone: a naturally occurring insecticide from the wild tomato lycopersicon hirsutum f.glabratum. | a nonalkaloid insecticide was isolated from the wild tomato lycopersicon hirsutum f. glabratum and identified as 2-tridecanone, a compound 72 times more abundant in the wild tomato than in the cultivated tomato l. esculentum. lepidopterous larvae (manduca sexta and heliothis zea) and aphids (aphis gossypii) died when confined on 2-tridecanone-treated filter paper. | 1980 | 17729870 |
| heterologous expression of methylketone synthase1 and methylketone synthase2 leads to production of methylketones and myristic acid in transgenic plants. | some plants produce methylketones as potent defense compounds against various insects. wild tomato (solanum habrochaites), a relative of the cultivated tomato (solanum lycopersicum), synthesizes large amounts of 2-methylketones in its glandular trichomes, but cultivated tomato trichomes contain little or no methylketones. two enzymes, solanum habrochaites methylketone synthase1 (shmks1) and shmks2, are required to convert β-ketoacyl acyl-carrier protein intermediates of the fatty acid biosynthet ... | 2014 | 24390393 |
| candidate genes and quantitative trait loci affecting fruit ascorbic acid content in three tomato populations. | fresh fruit and vegetables are a major source of ascorbic acid (vitamin c), an important antioxidant for the human diet and also for plants. ascorbic acid content in fruit exhibits a quantitative inheritance. quantitative trait loci (qtl) for ascorbic acid content have been mapped in three tomato populations derived from crosses between cultivated tomato varieties (solanum lycopersicum accessions) and three related wild species or subspecies. the first population consists of a set of introgressi ... | 2007 | 17277090 |
| the construction of a solanum habrochaites lyc4 introgression line population and the identification of qtls for resistance to botrytis cinerea. | tomato (solanum lycopersicum) is susceptible to grey mold (botrytis cinerea). partial resistance to this fungus has been identified in accessions of wild relatives of tomato such as solanum habrochaites lyc4. in a previous f(2) mapping study, three qtls conferring resistance to b. cinerea (rbcq1, rbcq2 and rbcq4a) were identified. as it was probable that this study had not identified all qtls involved in resistance we developed an introgression line (il) population (n = 30), each containing a s. ... | 2007 | 17273845 |
| occurrence of colombian datura virus in brugmansia hybrids, physalis peruviana l. and solanum muricatum ait. in hungary. | colombian datura virus (cdv) has been found to infect angel trumpets (brugmansia spp.) frequently and cape gooseberry (physalis peruviana) and pepino (solanum muricatum) sporadically in hungary. a cdv brg/h isolate was characterized. it had flexuous thread-like virions of about 750 x 12 nm in size. host range and symptomathological studies revealed its great similarity to authentic cdv isolates. nicotiana tabacum cultivars and lines resistant to potato virus y (pvyn) either genically or transgen ... | 2005 | 16047739 |
| metabolic, genomic, and biochemical analyses of glandular trichomes from the wild tomato species lycopersicon hirsutum identify a key enzyme in the biosynthesis of methylketones. | medium-length methylketones (c7-c15) are highly effective in protecting plants from numerous pests. we used a biochemical genomics approach to elucidate the pathway leading to synthesis of methylketones in the glandular trichomes of the wild tomato lycopersicon hirsutum f glabratum (accession pi126449). a comparison of gland est databases from accession pi126449 and a second l. hirsutum accession, la1777, whose glands do not contain methylketones, showed that the expression of genes for fatty ac ... | 2005 | 15772286 |
| mapping, genetic effects, and epistatic interaction of two bacterial canker resistance qtls from lycopersicon hirsutum. | two quantitative trait loci (qtl) from lycopersicon hirsutum, rcm 2.0 and rcm 5.1, control resistance to clavibacter michiganensis subsp. michiganensis ( cmm). to precisely map both loci, we applied interval mapping techniques to 1,056 individuals in three populations exhibiting f(2) segregation. based on a 1-lod confidence interval, rcm 2.0 mapped to a 14.9-cm interval on chromosome 2 and accounted for 25.7-34.0% of the phenotypic variation in disease severity. rcm 5.1 mapped to a 4.3-cm interv ... | 2004 | 15067391 |
| mutations in potato virus y genome-linked protein determine virulence toward recessive resistances in capsicum annuum and lycopersicon hirsutum. | the recessive resistance genes pot-1 and pvr2 in lycopersicon hirsutum and capsicum annuum, respectively, control potato virus y (pvy) accumulation in the inoculated leaves. infectious cdna molecules from two pvy isolates differing in their virulence toward these resistances were obtained using two different strategies. chimeras constructed with these cdna clones showed that a single nucleotide change corresponding to an amino acid substitution (arg119his) in the central part of the viral protei ... | 2004 | 15000399 |
| identification of qtls for early blight ( alternaria solani) resistance in tomato using backcross populations of a lycopersicon esculentum x l. hirsutum cross. | most commercial cultivars of tomato, lycopersicon esculentum mill., are susceptible to early blight (eb), a devastating fungal ( alternaria solani sorauer) disease of tomato in the northern and eastern parts of the u.s. and elsewhere in the world. the disease causes plant defoliation, which reduces yield and fruit quality, and contributes to significant crop loss. sources of resistance have been identified within related wild species of tomato. the purpose of this study was to identify and valid ... | 2002 | 12582599 |
| ancient origin of pathogen recognition specificity conferred by the tomato disease resistance gene pto. | we have investigated the origin of the pto disease resistance (r) gene that was previously identified in the wild tomato species lycopersicon pimpinellifolium and isolated by map-based cloning. pto encodes a serine-threonine protein kinase that specifically recognizes strains of pseudomonas syringae pv. tomato (pst) that express the avirulence gene avrpto. we examined an accession of the distantly related wild species lycopersicon hirsutum var. glabratum that exhibits avrpto-specific resistance ... | 2001 | 11172075 |
| structural basis for the recognition-evasion arms race between tomato mosaic virus and the resistance gene tm-1. | the tomato mosaic virus (tomv) resistance gene tm-1 encodes a protein that shows no sequence homology to functionally characterized proteins. tm-1 binds tomv replication proteins and thereby inhibits replication complex formation. tomv mutants that overcome this resistance have amino acid substitutions in the helicase domain of the replication proteins (tomv-hel). a small region of tm-1 in the genome of the wild tomato solanum habrochaites has been under positive selection during its antagonisti ... | 2014 | 25092327 |
| identification of a short rdna spacer sequence highly specific of a tomato line containing tm-1 gene introgressed from lycopersicon hirsutum. | we studied rdna restriction fragment length polymorphism between two tomato lines used for f1 hybrid seed production: line a, containing the tm-1 gene responsible for tobacco mosaic virus tolerance introgressed from the wild species lycopersicon hirsutum, and line b, a tobacco mosaic virus sensitive line. hybridization patterns led to distinct rdna maps with two size classes, 10.4 and 10.7 kb, in line a and a single, 8.9-kb class in line b. size differences were located in the intergenie sequenc ... | 1990 | 24221065 |
| an atl78-like ring-h2 finger protein confers abiotic stress tolerance through interacting with rav2 and csn5b in tomato. | ring finger proteins play an important role in plant adaptation to abiotic stresses. in the present study, a wild tomato (solanum habrochaites) cold-induced ring-h2 finger gene, shatl78l, was isolated, which has been identified as an abiotic stress responsive gene in tomato. the results showed that shatl78l was constitutively expressed in various tissues such as root, leaf, petiole, stem, flower, and fruit. cold stress up-regulated shatl78l in the cold-tolerant s. habrochaites compared to the su ... | 2016 | 27621744 |
| pto3 and pto4: novel genes from lycopersicon hirsutum var. glabratum that confer resistance to pseudomonas syringae pv tomato. | accessions of wild lycopersicon germplasm were screened for resistance to pseudomonas syringae pv tomato (p.s. tomato). resistance to both race-0 and race-1 strains of p.s. tomato was identified in l. pimpinellifolium, l. peruvianum and l. hirsutum var. glabratum. resistance to race-0 derived from l. hirsutum var. glabratum (pto3) appeared to be inherited independently of pto1 and pto2. filial and backcross generations derived from interspecific crosses between l. esculentum and l. hirsutum var. ... | 1994 | 24178099 |
| [somatic conversion (paramutation) at the sulfurea locus of lycopersicon esculentum mill. : iv. the genotypic determination of the frequency of conversion]. | 1. several lines of lycopersicon esculentum, which are heterozygous for a mutant sulfurea (sulf) allele vary greatly in the percentage of variegated plants among the heterozygotes. this variegation is caused by somatic conversion (paramutation). the different frequency of conversion is due to the presence of different sulf alleles. within the sulfurea (pura) (sulf (pura) ) and sulfurea (variegata) (sulf (vag) ) groups there are different alleles, which - though indistinguishable in homozygous co ... | 1969 | 24435556 |
| identification of qtls controlling resistance to pseudomonas syringae pv. tomato race 1 strains from the wild tomato, solanum habrochaites la1777. | screening of wild tomato accessions revealed a source of resistance to pseudomonas syringe pv. tomato race 1 from solanum habrochaites and facilitated mapping of qtls controlling disease resistance. pseudomonas syringae pv. tomato (pst) causes bacterial speck of tomato, which is one of the most persistent bacterial diseases in tomato worldwide. existing pst populations have overcome genetic resistance mediated by the tomato genes pto and prf. the objective of this study was to identify sources o ... | 2015 | 25634105 |
| a tilling approach to generate broad-spectrum resistance to potyviruses in tomato is hampered by eif4e gene redundancy. | genetic resistance to pathogens is important for sustainable maintenance of crop yields. recent biotechnologies offer alternative approaches to generate resistant plants by compensating for the lack of natural resistance. tomato (solanum lycopersicum) and related species offer a model in which natural and tilling-induced potyvirus resistance alleles may be compared. for resistance based on translation initiation factor eif4e1, we confirm that the natural allele sh-eif4e1(pi24)-pot1, isolated fro ... | 2016 | 26850324 |
| synthesis of methyl ketones by metabolically engineered escherichia coli. | methyl ketones are a group of highly reduced platform chemicals with widespread applications in the fragrance, flavor and pharmacological industries. current methods for the industrial production of methyl ketones include oxidation of hydrocarbons, but recent advances in the characterization of methyl ketone synthases from wild tomato have sparked interest towards the development of microbial platforms for the industrial production of methyl ketones. a functional methyl ketone biosynthetic pathw ... | 2012 | 22850984 |
| engineering of bacterial methyl ketone synthesis for biofuels. | we have engineered escherichia coli to overproduce saturated and monounsaturated aliphatic methyl ketones in the c(11) to c(15) (diesel) range; this group of methyl ketones includes 2-undecanone and 2-tridecanone, which are of importance to the flavor and fragrance industry and also have favorable cetane numbers (as we report here). we describe specific improvements that resulted in a 700-fold enhancement in methyl ketone titer relative to that of a fatty acid-overproducing e. coli strain, inclu ... | 2012 | 22038610 |
| resistance to tomato yellow leaf curl virus accumulation in the tomato wild relative solanum habrochaites associated with the c4 viral protein. | tomato yellow leaf curl disease (tylcd) is a severe threat to tomato crops worldwide and is caused by tomato yellow leaf curl virus (tylcv) and several other begomoviruses (genus begomovirus, family geminiviridae). host plant resistance is the best tylcd control method but limited sources of resistance are available. in this study, two solanum habrochaites tylcd-resistance sources, eelm-388 and eelm-889, were found after a wide germplasm screening and were further characterized. a consistent res ... | 2011 | 21405986 |
| coevolution and hierarchical interactions of tomato mosaic virus and the resistance gene tm-1. | during antagonistic coevolution between viruses and their hosts, viruses have a major advantage by evolving more rapidly. nevertheless, viruses and their hosts coexist and have coevolved, although the processes remain largely unknown. we previously identified tm-1 that confers resistance to tomato mosaic virus (tomv), and revealed that it encodes a protein that binds tomv replication proteins and inhibits rna replication. tm-1 was introgressed from a wild tomato species solanum habrochaites into ... | 2012 | 23093939 |
| an efficient in vitro-inoculation method for tomato yellow leaf curl virus. | tomato yellow leaf curl virus (tylcv) is a member of the family geminiviridae, genus begomovirus. to test the infectivity of tylcv in tomato plants, an improved protocol for inoculation of in vitro-cultured tomato plants was developed. | 2010 | 20429892 |
| resistance in tomato and wild relatives to crown and root rot caused by phytophthora capsici. | phytophthora capsici causes root, crown, and fruit rot of tomato, a major vegetable crop grown worldwide. the objective of this study was to screen tomato cultivars and wild relatives of tomato for resistance to p. capsici. four p. capsici isolates were individually used to inoculate 6-week-old seedlings (1 g of p. capsici-infested millet seed per 10 g of soilless medium) of 42 tomato cultivars and wild relatives of tomato in a greenhouse. plants were evaluated daily for wilting and death. all p ... | 2010 | 20465418 |
| multiple qtl for horticultural traits and quantitative resistance to phytophthora infestans linked on solanum habrochaites chromosome 11. | previously, a phytophthora infestans resistance qtl from solanum habrochaites chromosome 11 was introgressed into cultivated tomato (s. lycopersicum). fine mapping of this resistance qtl using near-isogenic lines (nils) revealed some co-located qtl with undesirable effects on plant size, canopy density, and fruit size traits. subsequently, higher-resolution mapping with sub-nils detected multiple p. infestans resistance qtl within this 9.4-cm region of chromosome 11. in our present study, these ... | 2014 | 25504736 |
| linkage relationships among multiple qtl for horticultural traits and late blight (p. infestans) resistance on chromosome 5 introgressed from wild tomato solanum habrochaites. | when the allele of a wild species at a quantitative trait locus (qtl) conferring a desirable trait is introduced into cultivated species, undesirable effects on other traits may occur. these negative phenotypic effects may result from the presence of wild alleles at other closely linked loci that are transferred along with the desired qtl allele (i.e., linkage drag) and/or from pleiotropic effects of the desired allele. previously, a qtl for resistance to phytophthora infestans on chromosome 5 o ... | 2013 | 24122052 |
| qtl analysis of quantitative resistance to phytophthora infestans (late blight) in tomato and comparisons with potato. | quantitative trait loci (qtls) for resistance to phytophthora infestans (late blight) were mapped in tomato. reciprocal backcross populations derived from cultivated lycopersicon esculentum x wild lycopersicon hirsutum (bc-e, backcross to l. esculentum; bc-h, backcross to l. hirsutum) were phenotyped in three types of replicated disease assays (detached-leaflet, whole-plant, and field). linkage maps were constructed for each bc population with rflps. resistance qtls were identified on all 12 tom ... | 2004 | 15190365 |
| wild tomato leaf extracts for spider mite and cowpea aphid control. | glandular trichomes on the leaves of wild tomato, l. hirsutum f. hirsutum mull, also known as solanum habrochaites (solanaceae), synthesize and accumulate high levels of methyl ketones (mks). l. hirsutum accession la 407, having high concentration of mks, was grown from seeds under greenhouse conditions. four mks (2-undecanone, 2-dodecanone, 2-tridecanone, and 2-pentadecanone) were screened for their toxicity to spider mites, tetranychus urticae koch and cowpea aphids, aphis craccivora koch. the ... | 2014 | 24813988 |
| establishment of an in vitro method for evaluating whitefly resistance in tomato. | an accurate and simple evaluation method is crucial for identifying whitefly resistance in tomato breeding. we developed an in vitro method for evaluating resistance of tomato leaves and tested this on wild and cultivated tomato varieties. we found that young leaves observed for whitefly oviposition after 8 hours provided appropriate comparative conditions. this method effectively distinguished resistance among tomato cultivars and wild species and also demonstrated significant difference in ovi ... | 2013 | 24273418 |
| tomato-produced 7-epizingiberene and r-curcumene act as repellents to whiteflies. | how whiteflies (bemisia tabaci) make the choice for a host plant prior to landing, is not precisely known. here we investigated whether they respond to specific volatiles of tomato. zingiberene and curcumene were purified from solanum habrochaites (pi127826), characterised by nmr and x-ray analysis and identified as 7-epizingiberene and r-curcumene. in contrast, oil from zingiber officinalis contained the stereoisomers zingiberene and s-curcumene, respectively. using a combination of free-choice ... | 2011 | 21074818 |
| sesquiterpene carboxylic acids from a wild tomato species affect larval feeding behavior and survival of helicoverpa zea and spodoptera exigua (lepidoptera: noctuidae). | the sesquiterpene carboxylic acids (sca), (+)-(e)-alpha-santalen-12-oic, (-)-(e)-endo-alpha-bergamoten-12-oic, and (+)-(e)-endo-beta-bergamoten-12-oic acid, are produced in glandular trichomes of lycopersicon hirsutum f. typicum humb. & bonpl. accession (la) 1777, which is highly resistant to a range of pests of cultivated tomatoes. l. esculentum mill. exposure of the larvae of two key tomato pests, tomato fruitworm [helicoverpa zea (boddie)] and beet armyworm [spodoptera exigua (hübner)], to th ... | 2001 | 11681691 |
| relative mass defect filtering of mass spectra: a path to discovery of plant specialized metabolites. | the rapid identification of novel plant metabolites and assignments of newly discovered substances to natural product classes present the main bottlenecks to defining plant specialized phenotypes. although mass spectrometry provides powerful support for metabolite discovery by measuring molecular masses, ambiguities in elemental formulas often fail to reveal the biosynthetic origins of specialized metabolites detected using liquid chromatography-mass spectrometry. a promising approach for mining ... | 2015 | 25659383 |
| identification, introgression, and validation of fruit volatile qtls from a red-fruited wild tomato species. | volatile organic compounds (vocs) are major determinants of fruit flavor, a primary objective in tomato breeding. a recombinant inbred line (ril) population consisting of 169 lines derived from a cross between solanum lycopersicum and a red-fruited wild tomato species solanum pimpinellifolium accession (sp) was characterized for vocs in three different seasons. correlation and hierarchical cluster analyses were performed on the 52 vocs identified, providing a tool for the putative assignation of ... | 2016 | 28040800 |
| diverse responses of wild and cultivated tomato to baba, oligandrin and oidium neolycopersici infection. | current strategies for increased crop protection of susceptible tomato plants against pathogen infections include treatment with synthetic chemicals, application of natural pathogen-derived compounds or transfer of resistance genes from wild tomato species within breeding programmes. in this study, a series of 45 genes potentially involved in defence mechanisms was retrieved from the genome sequence of inbred reference tomato cultivar solanum lycopersicum 'heinz 1706'. the aim of the study was t ... | 2017 | 27660055 |
| identification and ds-tagged isolation of a new gene at the cf-4 locus of tomato involved in disease resistance to cladosporium fulvum race 5. | leaf mould disease in tomato is caused by the biotrophic fungus cladosporium fulvum. an ac/ds targeted transposon tagging strategy was used to isolate the gene conferring resistance to race 5 of c. fulvum, a strain expressing the avirulence gene avr4. an infection assay of 2-week-old seedlings yielded five susceptible mutants, of which two had a ds element integrated in the same gene at different positions. this gene, member of a gene family, showed high sequence homology to the c. fulvum resist ... | 1998 | 9670557 |
| gene expression analysis of solanum lycopersicum and solanum habrochaites under drought conditions. | drought is one of the limiting environmental factors that affect crop production worldwide. understanding the molecular mechanism of drought stress is the key to developing drought tolerant crop. in this experiment we performed expression profiling of tomato plants under water deficit conditions using microarray technology. the data set we generated (available in the ncbi/geo database under gse22304) has been analyzed to identify genes that are involved in the regulation of tomato's responses to ... | 2016 | 27408808 |
| current status of early blight resistance in tomato: an update. | early blight (eb) is one of the dreadful diseases of tomato caused by several species of alternaria including alternaria linariae (which includes a. solani and a. tomatophila), as well as a. alternata. in some instances, annual economic yield losses due to eb have been estimated at 79%. alternaria are known only to reproduce asexually, but a highly-virulent isolate has the potential to overcome existing resistance genes. currently, cultural practices and fungicide applications are employed for t ... | 2017 | 28934121 |
| ht proteins contribute to s-rnase-independent pollen rejection in solanum. | plants have mechanisms to recognize and reject pollen from other species. although widespread, these mechanisms are less well understood than the self-incompatibility (si) mechanisms plants use to reject pollen from close relatives. previous studies have shown that some interspecific reproductive barriers (irbs) are related to si in the solanaceae. for example, the pistil si proteins s-rnase and ht protein function in a pistil-side irb that causes rejection of pollen from self-compatible (sc) re ... | 2017 | 27862494 |
| exploring genetic variation in the tomato (solanum section lycopersicon) clade by whole-genome sequencing. | we explored genetic variation by sequencing a selection of 84 tomato accessions and related wild species representative of the lycopersicon, arcanum, eriopersicon and neolycopersicon groups, which has yielded a huge amount of precious data on sequence diversity in the tomato clade. three new reference genomes were reconstructed to support our comparative genome analyses. comparative sequence alignment revealed group-, species- and accession-specific polymorphisms, explaining characteristic fruit ... | 2014 | 25039268 |
| evidence of cryptic introgression in tomato (solanum lycopersicum l.) based on wild tomato species alleles. | many highly beneficial traits (e.g. disease or abiotic stress resistance) have been transferred into crops through crosses with their wild relatives. the 13 recognized species of tomato (solanum section lycopersicon) are closely related to each other and wild species genes have been extensively used for improvement of the crop, solanum lycopersicum l. in addition, the lack of geographical barriers has permitted natural hybridization between s. lycopersicum and its closest wild relative solanum p ... | 2012 | 22871151 |
| comparative functional genomic analysis of solanum glandular trichome types. | glandular trichomes play important roles in protecting plants from biotic attack by producing defensive compounds. we investigated the metabolic profiles and transcriptomes to characterize the differences between different glandular trichome types in several domesticated and wild solanum species: solanum lycopersicum (glandular trichome types 1, 6, and 7), solanum habrochaites (types 1, 4, and 6), solanum pennellii (types 4 and 6), solanum arcanum (type 6), and solanum pimpinellifolium (type 6). ... | 2011 | 21098679 |
| mineral and trace elements content in 30 accessions of tomato fruits (solanum lycopersicum l.,) and wild relatives (solanum pimpinellifolium l., solanum cheesmaniae l. riley, and solanum habrochaites s. knapp & d.m. spooner). | tomato quality and its potential health benefits are directly related to its chemical composition. the characterization of nutritional properties of solanum germplasm is essential to choose suitable donor parents for breeding programs. in this sense, wild species could be very useful for tomato fruit quality genetic improvement. with this objective, in this work, we characterize micronutrients content in eulycopersicon germplasm (20 cultivars of s. lycopersicum l. and 10 accessions of wild relat ... | 2011 | 20526750 |
| the identification of a gene (cwp1), silenced during solanum evolution, which causes cuticle microfissuring and dehydration when expressed in tomato fruit. | one of the most intriguing phenomena of fleshy fruit is the ability to maintain high water content at maturity, even following harvest. this is accomplished by a fruit cuticle that is highly impermeable to water diffusion. in this paper, we report on a novel genotype of tomato, developed via introgression from the wild species solanum habrochaites, which is characterized by microfissuring of the fruit cuticle and dehydration of the mature fruit. the microfissure/dehydration phenotype is inherite ... | 2007 | 17877702 |
| ripening behavior of wild tomato species. | nine wild tomato species were surveyed for variability in ripening characteristics. external signs of ripening, age of fruit at ripening, and ethylene production patterns were compared. ethylene production was monitored using an ethylene-free air stream system and gas chromatography. based on these ripening characteristics, the fruits fell into three general categories: those that change color when they ripen, green-fruited species that abscise prior to ripening, and green-fruited species that r ... | 1981 | 16662121 |
| rapid identification of candidate genes for resistance to tomato late blight disease using next-generation sequencing technologies. | tomato late blight caused by phytophthora infestans (mont.) de bary, also known as the irish famine pathogen, is one of the most destructive plant diseases. wild relatives of tomato possess useful resistance genes against this disease, and could therefore be used in breeding to improve cultivated varieties. in the genome of a wild relative of tomato, solanum habrochaites accession la1777, we identified a new quantitative trait locus for resistance against blight caused by an aggressive egyptian ... | 2017 | 29253902 |
| green-fruited solanum habrochaites lacks fruit-specific carotenogenesis due to metabolic and structural blocks. | members of the tomato clade exhibit a wide diversity in fruit color, but the mechanisms governing inter-species diversity of coloration are largely unknown. the carotenoid profiles, carotenogenic gene expression and proteome profiles of green-fruited solanum habrochaites (sh), orange-fruited s. galapagense, and red-fruited s. pimpinellifolium were compared with cultivated tomato [s. lycopersicum cv. ailsa craig (sl)] to decipher the molecular basis of coloration diversity. green-fruited sh, thou ... | 2017 | 29048567 |
| rootstock sub-optimal temperature tolerance determines transcriptomic responses after long-term root cooling in rootstocks and scions of grafted tomato plants. | grafting of elite cultivars onto tolerant rootstocks is an advanced strategy to increase tomato tolerance to sub-optimal temperature. however, a detailed understanding of adaptive mechanisms to sub-optimal temperature in rootstocks and scions of grafting combinations on a physiological and molecular level is lacking. here, the commercial cultivar kommeet was grafted either onto 'moneymaker' (sensitive) or onto the line accession la 1777 of solanum habrochaites (tolerant). grafted plants were gro ... | 2017 | 28642763 |
| pervasive antagonistic interactions among hybrid incompatibility loci. | species barriers, expressed as hybrid inviability and sterility, are often due to epistatic interactions between divergent loci from two lineages. theoretical models indicate that the strength, direction, and complexity of these genetic interactions can strongly affect the expression of interspecific reproductive isolation and the rates at which new species evolve. nonetheless, empirical analyses have not quantified the frequency with which loci are involved in interactions affecting hybrid fitn ... | 2017 | 28604770 |
| grafting response to excess boron and expression analysis of genes coding boron transporters in tomato. | boron (b) is essential for plant growth, however its excess in soil and/or in irrigation water can severely compromise plant growth and yield. the goal of this work was to determine whether grafting onto 'arnold', a commercial interspecific hybrid (solanum lycopersicum × s. habrochaites) rootstock, which in a previous study was found to be tolerant to salt stress, could improve tomato (s. lycopersicum l. 'ikram') tolerance to excess b, and whether this effect is associated with an exclusion mech ... | 2017 | 28603945 |
| transcriptomic analysis links gene expression to unilateral pollen-pistil reproductive barriers. | unilateral incompatibility (ui) is an asymmetric reproductive barrier that unidirectionally prevents gene flow between species and/or populations. ui is characterized by a compatible interaction between partners in one direction, but in the reciprocal cross fertilization fails, generally due to pollen tube rejection by the pistil. although ui has long been observed in crosses between different species, the underlying molecular mechanisms are only beginning to be characterized. the wild tomato re ... | 2017 | 28438120 |
| multi-omics of tomato glandular trichomes reveals distinct features of central carbon metabolism supporting high productivity of specialized metabolites. | glandular trichomes are metabolic cell factories with the capacity to produce large quantities of secondary metabolites. little is known about the connection between central carbon metabolism and metabolic productivity for secondary metabolites in glandular trichomes. to address this gap in our knowledge, we performed comparative metabolomics, transcriptomics, proteomics, and 13c-labeling of type vi glandular trichomes and leaves from a cultivated (solanum lycopersicum la4024) and a wild (solanu ... | 2017 | 28408661 |
| differential transcriptional regulation in roots of tomato near-isogenic lines in response to rapid-onset water stress. | cultivated tomato (solanum lycopersicum l.) is susceptible to abiotic stresses, including drought and chilling stress, while its wild relative (solanum habrochaites) exhibits tolerance to many abiotic stresses. chilling roots to 6°c induces rapid-onset water stress by impeding water movement from roots to shoots. wild s. habrochaites responds to root chilling by closing stomata and maintaining shoot turgor, while cultivated tomato fails to close stomata and wilts. this phenotypic response (shoot ... | 2017 | 28270818 |
| in silico identification of snp diversity in cultivated and wild tomato species: insight from molecular simulations. | single nucleotide polymorphisms (snps), an important source of genetic variations, are often used in crop improvement programme. the present study represented comprehensive in silico analysis of nucleotide polymorphisms in wild (solanum habrochaites) and cultivated (solanum lycopersicum) species of tomato to explore the consequence of substitutions both at sequence and structure level. a total of 8978 snps having ts/tv (transition/transversion) ratio 1.75 were identified from the expressed seque ... | 2016 | 27929054 |
| solanum habrochaites, a potential source of resistance against bactericera cockerelli (hemiptera: triozidae) and "candidatus liberibacter solanacearum". | the potato psyllid, bactericera cockerelli sulc, also known as tomato psyllid, is a serious pest of solanaceous plants. its host selection criteria are poorly understood. in this study, we tested whether the solanum habrochaites (pi127826), a wild solanaceous plant known for its property to repel whiteflies, was repellent to potato psyllids. using a combination of nonchoice assays and choice assays on different psyllid stages, we demonstrated that s. habrochaites is both repelling and toxic to p ... | 2014 | 25026681 |
| towards the introducing of resistance to powdery mildew from lycopersicon hirsutum into l. esculentum. | genes of resistance to oidium lycopersicum from lycopersicon hirsutum la 1775 were introduced to l. esculentum. breeding procedures were based on a one-way programme up to the f2 generation and then four different methods were adopted to obtain f4 and bc4 populations. screening tests among those hybrid populations were performed in a greenhouse and showed segregation for resistance to powdery mildew due to different genetic backgrounds of the families derived from four breeding methods that chan ... | 2001 | 14564061 |
| characterization and mapping of resistance to oidium lycopersicum in two lycopersicon hirsutum accessions: evidence for close linkage of two ol-genes on chromosome 6 of tomato. | the cultivated tomato is susceptible to powdery mildew (oidium lycopersicum). several accessions of wild species are resistant. in this study we describe (i) the genetics and mapping of resistance to o. lycopersicum in g1.1290, one of the resistant accessions in lycopersicon hirsutum, (ii) fine mapping of ol-1 originated from l. hirsutum g1.1560, another resistant accession of l. hirsutum, and (iii) tests of allelism for resistance in g1.1290 and g1.1560. initially, it is demonstrated that the r ... | 2000 | 11240617 |
| resistance to powdery mildew (oidium lycopersicum) in lycopersicon hirsutum is controlled by an incompletely-dominant gene ol-1 on chromosome 6. | the inheritance of resistance to powdery mildew (oidium lycopersicum) in lycopersicon hirsutum was investigated by disease tests in segregating populations obtained by hybridising tomato (l. esculentum) cv moneymaker with the wild relative l. hirsutum g1.1560. one incompletely dominant gene ol-1 was found to largely control resistance to the disease. to map ol-1, dna pools from seven resistant and ten susceptible f2 plants were analyzed for random amplified polymorphic dna (rapd). with 32 primer ... | 1994 | 24177896 |
| discovering regulated metabolite families in untargeted metabolomics studies. | the identification of metabolites by mass spectrometry constitutes a major bottleneck which considerably limits the throughput of metabolomics studies in biomedical or plant research. here, we present a novel approach to analyze metabolomics data from untargeted, data-independent lc-ms/ms measurements. by integrated analysis of ms(1) abundances and ms/ms spectra, the identification of regulated metabolite families is achieved. this approach offers a global view on metabolic regulation in compara ... | 2016 | 27452369 |
| autofluorescence as a signal to sort developing glandular trichomes by flow cytometry. | the industrial relevance of a number of metabolites produced in plant glandular trichomes (gts) has spurred research on these specialized organs for a number of years. most of the research, however, has focused on the elucidation of secondary metabolite pathways and comparatively little has been undertaken on the development and differentiation of gts. one way to gain insight into these developmental processes is to generate stage-specific transcriptome and metabolome data. the difficulty for th ... | 2016 | 27446176 |
| indirect selection of industrial tomato genotypes rich in zingiberene and resistant to tuta absoluta meyrick. | obtaining tomato cultivars resistant to pests through interspecific crosses between commercial cultivars and wild accessions is an important tool in integrated pest management. the aim of this study was to select tomato genotypes with high zingiberene (zgb) levels that are resistant to the south american tomato moth (tuta absoluta meyrick) and to estimate genetic parameters of zgb inheritance from the interspecific cross solanum lycopersicum cultivar 'redenção' x solanum habrochaites var. hirsut ... | 2015 | 26634470 |
| protein modeling and molecular dynamics simulation of slwrky4 protein cloned from drought tolerant tomato (solanum habrochaites) line ec520061. | wrky genes are members of one of the largest families of plant transcription factors and play an important role in response to biotic and abiotic stresses, and overall growth and development. understanding the interaction of wrky proteins with other proteins/ligands in plant cells is of utmost importance to develop plants having tolerance to biotic and abiotic stresses. the slwrky4 gene was cloned from a drought tolerant wild species of tomato (solanum habrochaites) and the secondary structure a ... | 2015 | 26369915 |
| dependence of negative-mode electrospray ionization response factors on mobile phase composition and molecular structure for newly-authenticated neutral acylsucrose metabolites. | authentic standards of known concentrations serve as references for accurate absolute quantification of plant metabolites using liquid chromatography/mass spectrometry (lc/ms). however, often such standards are not commercially available or not amenable for custom syntheses. despite the widespread use of electrospray ionization for metabolite analyses, the fundamentals needed for reliable prediction of molecular response factors have yet to be explored in detail for analytes that lack ionized fu ... | 2015 | 26331907 |
| repellency and oviposition deterrence of wild tomato leaf extracts to spider mites, tetranychus urticae koch. | glandular trichomes on the leaves of wild tomato, lycopersicon hirsutum f. hirsutum mull, also known as solanum habrochaites (solanaceae) synthesize and accumulate high levels of methyl ketones (mks). the potential of using mks as alternatives to synthetic acaricides for controlling the twospotted spider mite, tetranychus urticae koch, is explored in this study. plants of l. hirsutum accession la 407 having high concentrations of mks were grown from seeds under greenhouse conditions. the main ob ... | 2015 | 26079341 |
| a comparison of the low temperature transcriptomes of two tomato genotypes that differ in freezing tolerance: solanum lycopersicum and solanum habrochaites. | solanum lycopersicum and solanum habrochaites are closely related plant species; however, their cold tolerance capacities are different. the wild species s. habrochaites is more cold tolerant than the cultivated species s. lycopersicum. | 2015 | 26048292 |
| high-resolution mapping of a major effect qtl from wild tomato solanum habrochaites that influences water relations under root chilling. | qtl stm9 controlling rapid-onset water stress tolerance in s. habrochaites was high-resolution mapped to a chromosome 9 region that contains genes associated with abiotic stress tolerances. wild tomato (solanum habrochaites) exhibits tolerance to abiotic stresses, including drought and chilling. root chilling (6 °c) induces rapid-onset water stress by impeding water movement from roots to shoots. s. habrochaites responds to such changes by closing stomata and maintaining shoot turgor, while cult ... | 2015 | 26044122 |
| functionally divergent alleles and duplicated loci encoding an acyltransferase contribute to acylsugar metabolite diversity in solanum trichomes. | glandular trichomes from tomato (solanum lycopersicum) and other species in the solanaceae produce and secrete a mixture of o-acylsugars (aliphatic esters of sucrose and glucose) that contribute to insect defense. despite their phylogenetic distribution and diversity, relatively little is known about how these specialized metabolites are synthesized. mass spectrometric profiling of acylsugars in the s. lycopersicum x solanum pennellii introgression lines identified a chromosome 11 locus containi ... | 2015 | 25862303 |
| overexpression of shdhn, a dehydrin gene from solanum habrochaites enhances tolerance to multiple abiotic stresses in tomato. | dehydrins (dhns) play important roles in plant adaptation to abiotic stress. in this study, a cold-induced sk3-type dhn gene (shdhn) isolated from wild tomato species solanum habrochaites was characterized for its function in abiotic stress tolerance. shdhn was constitutively expressed in root, leaf, stem, flower and fruit. shdhn was continuously up-regulated during cold stress and showed higher expression level in the cold-tolerant s. habrochaites than in the susceptible s. lycopersicum. moreov ... | 2015 | 25576005 |
| the cf-4 and cf-9 resistance genes against cladosporium fulvum are conserved in wild tomato species. | the cf-4 and cf-9 genes originate from the wild tomato species lycopersicon hirsutum and l. pimpinellifolium and confer resistance to strains of the leaf mold fungus cladosporium fulvum that secrete the avr4 and avr9 elicitor proteins, respectively. homologs of cf-4 and cf-9 (hcr9s) are located in several clusters and evolve mainly through sequence exchange between homologs. to study the evolution of cf genes, we set out to identify functional hcr9s that mediate recognition of avr4 and avr9 (des ... | 2005 | 16167771 |
| mating system transitions in solanum habrochaites impact interactions between populations and species. | in plants, transitions in mating system from outcrossing to self-fertilization are common; however, the impact of these transitions on interspecific and interpopulation reproductive barriers is not fully understood. we examined the consequences of mating system transition for reproductive barriers in 19 populations of the wild tomato species solanum habrochaites. we identified s. habrochaites populations with self-incompatible (si), self-compatible (sc) and mixed population (mp) mating systems, ... | 2017 | 27516156 |
| overexpression of calmodulin-like (shcml44) stress-responsive gene from solanum habrochaites enhances tolerance to multiple abiotic stresses. | calmodulin-like (cml) proteins are important ca(2+) sensors, which play significant role in mediating plant stress tolerance. in the present study, cold responsive calmodulin-like (shcml44) gene was isolated from cold tolerant wild tomato (solanum habrochaites), and functionally characterized. the shcml44 was differentially expressed in all plant tissues including root, stem, leaf, flower and fruit, and was strongly up-regulated under cold, drought and salinity stresses along with plant growth h ... | 2016 | 27546315 |
| mapping and characterization of novel parthenocarpy qtls in tomato. | parthenocarpy is the development of the fruit in absence of pollination and/or fertilization. in tomato, parthenocarpy is considered as an attractive trait to solve the problems of fruit setting under unfavorable conditions. we studied the genetics of parthenocarpy in two different lines, il5-1 and ivt-line 1, both carrying solanum habrochaites chromosome segments. parthenocarpy in il5-1 is under the control of two qtls, one on chromosome 4 (pat4.1) and one on chromosome 5 (pat5.1). ivt-line 1 a ... | 2008 | 18231773 |
| identification of chilling stress-responsive tomato micrornas and their target genes by high-throughput sequencing and degradome analysis. | micrornas (mirnas) are a class of noncoding small rnas (srnas) that are 20-24 nucleotides (nt) in length. extensive studies have indicated that mirnas play versatile roles in plants, functioning in processes such as growth, development and stress responses. chilling is a common abiotic stress that seriously affects plants growth and development. recently, chilling-responsive mirnas have been detected in several plant species. however, little is known about the mirnas in the model plant tomato. ' ... | 2014 | 25519760 |
| analysis of natural and induced variation in tomato glandular trichome flavonoids identifies a gene not present in the reference genome. | flavonoids are ubiquitous plant aromatic specialized metabolites found in a variety of cell types and organs. methylated flavonoids are detected in secreting glandular trichomes of various solanum species, including the cultivated tomato (solanum lycopersicum). inspection of the sequenced s. lycopersicum heinz 1706 reference genome revealed a close homolog of solanum habrochaites momt1 3'/5' myricetin o-methyltransferase gene, but this gene (solyc06g083450) is missing the first exon, raising the ... | 2014 | 25128240 |
| comparative structural profiling of trichome specialized metabolites in tomato (solanumlycopersicum) and s. habrochaites: acylsugar profiles revealed by uhplc/ms and nmr. | many plants accumulate large quantities of specialized metabolites in secretory glandular trichomes (sgts), which are specialized epidermal cells. in the genus solanum, sgts store a diverse collection of glucose and sucrose esters. profiling of extracts from two accessions (la1777 and la1392) of solanum habrochaites using ultra-high performance liquid chromatography-mass spectrometry (uhplc/ms) revealed wide acylsugar diversity, with up to 11 isomers annotated for each individual elemental formu ... | 2014 | 24772058 |
| isolation and functional characterization of the shcbf1 gene encoding a crt/dre-binding factor from the wild tomato species solanum habrochaites. | plant growth and productivity are greatly affected by low ambient temperature. complex cascades of gene expression in cold stress response are regulated by transcription factors. in this study, a cdna clone, named shcbf1, was isolated from solanum habrochaites seedlings (a wild relative of cultivated tomato). it was classified as one of cbf family members based on multiple sequence alignment. the expression analysis confirmed that shcbf1 was induced by low temperature, high salinity and drought ... | 2014 | 24333684 |
| sesquiterpenoid glycosides from glandular trichomes of the wild tomato relative solanum habrochaites. | profiles of terpenoid glycoside metabolites in glandular trichomes of solanum habrochaites la1777 leaves were generated using ultrahigh performance liquid chromatography/time-of-flight mass spectrometry with multiplexing of non-selective collision-induced dissociation (cid). profiling data suggested a diverse group of 52 sesquiterpenoid glycosides, and fragment ions observed in both non-selective cid mass spectra and true tandem mass spectrometry (ms/ms) product ion spectra documented variation ... | 2014 | 24333030 |
| determination of residues responsible for substrate and product specificity of solanum habrochaites short-chain cis-prenyltransferases. | isoprenoids are diverse compounds that have their biosynthetic origin in the initial condensation of isopentenyl diphosphate and dimethylallyl diphosphate to form c10 prenyl diphosphates that can be elongated by the addition of subsequent isopentenyl diphosphate units. these reactions are catalyzed by either cis-prenyltransferases (cpts) or trans-prenyltransferases. the synthesis of volatile terpenes in plants typically proceeds through either geranyl diphosphate (c10) or trans-farnesyl diphosph ... | 2014 | 24254315 |
| chemical imaging of trichome specialized metabolites using contact printing and laser desorption/ionization mass spectrometry. | cell transfer by contact printing coupled with carbon-substrate-assisted laser desorption/ionization was used to directly profile and image secondary metabolites in trichomes on leaves of the wild tomato solanum habrochaites. major specialized metabolites, including acyl sugars, alkaloids, flavonoids, and terpenoid acids, were successfully detected in positive ion mode or negative ion mode, and in some cases in both modes. this simple solvent-free and matrix-free sample preparation for mass spec ... | 2014 | 24220760 |
| genotypic variability for antioxidant and quality parameters among tomato cultivars, hybrids, cherry tomatoes and wild species. | wide germplasm diversity and transferability of antioxidant parameters is the primary requirement for the development of high-antioxidant tomato cultivars. the present study was conducted to screen tomato genotypes including hybrids, varieties, cherry tomatoes, wild species, elite germplasm lines, interspecific hybrids and backcross populations for antioxidant activity and other quality parameters to select high-antioxidant lines with good total soluble solids (tss) for further usage in crop imp ... | 2014 | 24037905 |
| evolution of a complex locus for terpene biosynthesis in solanum. | functional gene clusters, containing two or more genes encoding different enzymes for the same pathway, are sometimes observed in plant genomes, most often when the genes specify the synthesis of specialized defensive metabolites. here, we show that a cluster of genes in tomato (solanum lycopersicum; solanaceae) contains genes for terpene synthases (tpss) that specify the synthesis of monoterpenes and diterpenes from cis-prenyl diphosphates, substrates that are synthesized by enzymes encoded by ... | 2013 | 23757397 |
| mutations in two pollen self-incompatibility factors in geographically marginal populations of solanum habrochaites impact mating system transitions and reproductive isolation. | self-incompatibility (si) is a mechanism that prevents inbreeding in many plant species. the mutational breakdown of si occurs frequently, yet relatively little is known about the evolutionary steps involved in the progressive loss of pistil and pollen si function. | 2016 | 27793860 |
| development of a set of near isogenic and backcross recombinant inbred lines containing most of the lycopersicon hirsutum genome in a l. esculentum genetic background: a tool for gene mapping and gene discovery. | a novel population consisted of a set of 99 near isogenic lines (nils) and backcross recombinant inbred lines (bcrils) derived from a cross between the cultivated tomato lycopersicon esculentum cv. e6206 and l. hirsutum accession la1777 is presented. most of the lines contain a single defined introgression from l. hirsutum in the l. esculentum genetic background and together, the lines provide a coverage of more than the 85% of the l. hirsutum genome. these lines represent a new tool to uncover ... | 2000 | 11081970 |
| emergent decarboxylase activity and attenuation of α/β-hydrolase activity during the evolution of methylketone biosynthesis in tomato. | specialized methylketone-containing metabolites accumulate in certain plants, in particular wild tomatoes in which they serve as toxic compounds against chewing insects. in solanum habrochaites f. glabratum, methylketone biosynthesis occurs in the plastids of glandular trichomes and begins with intermediates of de novo fatty acid synthesis. these fatty-acyl intermediates are converted via sequential reactions catalyzed by methylketone synthase2 (mks2) and mks1 to produce the n-1 methylketone. we ... | 2012 | 22523203 |
| the fruit cuticles of wild tomato species exhibit architectural and chemical diversity, providing a new model for studying the evolution of cuticle function. | the cuticle covers the aerial epidermis of land plants and plays a primary role in water regulation and protection from external stresses. remarkable species diversity in the structure and composition of its components, cutin and wax, have been catalogued, but few functional or genetic correlations have emerged. tomato (solanum lycopersicum) is part of a complex of closely related wild species endemic to the northern andes and the galapagos islands (solanum sect. lycopersicon). although sharing ... | 2012 | 22007785 |
| 2,3-dihydrofarnesoic acid, a unique terpene from trichomes oflycopersicon hirsutum, repels spider mites. | lycopersicon hirsutum, a wild relative of the tomato, is highly resistant to arthropod herbivores. both botanic forms ofl. hirsutum, l. hirsutum f.glabratum (c.h. mull.) andl. hirsutum f.typicum (humb. & bonpl.), are resistant to two-spotted spider mites,tetranychus urticae koch. however, leaves and trichome secretions from f.typicum repel mites more so than those from f.glabratum. we have previously demonstrated that trichome secretions from la 1363 and la 1927, accessions of f.typicum, repelle ... | 1993 | 24248790 |
| rna-seq discovery, functional characterization, and comparison of sesquiterpene synthases from solanum lycopersicum and solanum habrochaites trichomes. | solanum lycopersicum and solanum habrochaites (f. typicum) accession pi127826 emit a variety of sesquiterpenes. to identify terpene synthases involved in the production of these volatile sesquiterpenes, we used massive parallel pyrosequencing (rna-seq) to obtain the transcriptome of the stem trichomes from these plants. this approach resulted initially in the discovery of six sesquiterpene synthase cdnas from s. lycopersicum and five from s. habrochaites. searches of other databases and the s. l ... | 2011 | 21818683 |
| a sensitive bioassay for spider mite (tetranychus urticae) repellency: a double bond makes a difference. | choice bioassays were used to determine repellency of homologous n-alkanes (c(8)h(18)-c(21)h(44)) to spider mites. when tested at 400 μg/cm(2), the c(15)-c(19) alkanes were highly repellent; the c(16) n-alkane, n-hexadecane, was most repellent. subsequently the ec(50) values, the concentration at which 50% of the mites were repelled, were determined for the c(15)-c(19) n-alkanes and their analogous 1-n-alkenes (c(15)h(30)-c(19)h(38)). the ec(50) value for 1-heptadecene, the c(17) 1-n-alkene, was ... | 2011 | 21761225 |