| natural variation in tomato reveals differences in the recognition of avrpto and avrptob effectors from pseudomonas syringae. | the pto protein kinase from solanum pimpinellifolium interacts with pseudomonas syringae effectors avrpto or avrptob to activate effector-triggered immunity. the previously solved crystal structures of the avrpto-pto and avrptob-pto complexes revealed that pto binds each effector through both a shared and a unique interface. here we use natural variation in wild species of tomato to further investigate pto recognition of these two effectors. one species, solanum chmielewskii, was found to have m ... | 2016 | 26993968 |
| identification of loci affecting accumulation of secondary metabolites in tomato fruit of a solanum lycopersicum × solanum chmielewskii introgression line population. | semi-polar metabolites such as flavonoids, phenolic acids, and alkaloids are very important health-related compounds in tomato. as a first step to identify genes responsible for the synthesis of semi-polar metabolites, quantitative trait loci (qtls) that influence the semi-polar metabolite content in red-ripe tomato fruit were identified, by characterizing fruits of a population of introgression lines (ils) derived from a cross between the cultivated tomato solanum lycopersicum and the wild spec ... | 0 | 27733856 |
| nitric oxide and reactive oxygen species regulate the accumulation of heat shock proteins in tomato leaves in response to heat shock and pathogen infection. | heat shock proteins (hsp) are produced in response to various stress stimuli to prevent cell damage. we evaluated the involvement of nitric oxide (no) and reactive oxygen species (ros) in the accumulation of hsp70 proteins in tomato leaves induced by abiotic and biotic stress stimuli. a model system of leaf discs was used with two tomato genotypes, solanum lycopersicum cv. amateur and solanum chmielewskii, differing in their resistance to fungal pathogen oidium neolycopersici. leaf discs were ex ... | 2013 | 23602099 |
| evolutionary history of two pollen self-incompatibility factors reveals alternate routes to self-compatibility within solanum. | self-incompatibility (si) prevents self-fertilization and reduces inbreeding. while si is common in plants, transitions to self-compatibility (sc) occur frequently. little is known about the genetic changes and evolutionary steps underlying these shifts. | 2017 | 29212768 |
| genetic structure of the four wild tomato species in the solanum peruvianum s.l. species complex. | the most diverse wild tomato species solanum peruvianum sensu lato (s.l.) has been reclassified into four separate species: solanum peruvianum sensu stricto (s.s.), solanum corneliomuelleri, solanum huaylasense, and solanum arcanum. however, reproductive barriers among the species are incomplete and this can lead to discrepancies regarding genetic identity of germplasm. we used genotyping by sequencing (gbs) of s. peruvianum s.l., solanum neorickii, and solanum chmielewskii to develop tens of th ... | 2014 | 24884691 |
| ripening behavior of wild tomato species. | nine wild tomato species were surveyed for variability in ripening characteristics. external signs of ripening, age of fruit at ripening, and ethylene production patterns were compared. ethylene production was monitored using an ethylene-free air stream system and gas chromatography. based on these ripening characteristics, the fruits fell into three general categories: those that change color when they ripen, green-fruited species that abscise prior to ripening, and green-fruited species that r ... | 1981 | 16662121 |
| combining ecophysiological modelling and quantitative trait locus analysis to identify key elementary processes underlying tomato fruit sugar concentration. | a mechanistic model predicting the accumulation of tomato fruit sugars was developed in order (i) to dissect the relative influence of three underlying processes: assimilate supply (s), metabolic transformation of sugars into other compounds (m), and dilution by water uptake (d); and (ii) to estimate the genetic variability of s, m, and d. the latter was estimated in a population of 20 introgression lines derived from the introgression of a wild tomato species (solanum chmielewskii) into s. lyco ... | 2011 | 21036926 |
| the influence of fruit load on the tomato pericarp metabolome in a solanum chmielewskii introgression line population. | it has been recently demonstrated, utilizing interspecific introgression lines of tomato, generated from the cross between solanum lycopersicum and the wild species solanum pennellii, that the efficiency of photosynthate partitioning exerts a considerable influence on the metabolic composition of tomato fruit pericarp. in order to further evaluate the influence of source-sink interaction, metabolite composition was determined by gas chromatography-mass spectrometry in a different population. for ... | 2010 | 20841452 |
| genotype-dependent response to carbon availability in growing tomato fruit. | tomato fruit growth and composition depend on both genotype and environment. this paper aims at studying how fruit phenotypic responses to changes in carbon availability can be influenced by genotype, and at identifying genotype-dependent and -independent changes in gene expression underlying variations in fruit growth and composition. we grew a parental line (solanum lycopersicum) and an introgression line from solanum chmielewskii harbouring quantitative trait loci for fresh weight and sugar c ... | 2010 | 20199617 |
| biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor slmyb12 leads to pink tomato fruit color. | the color of tomato fruit is mainly determined by carotenoids and flavonoids. phenotypic analysis of an introgression line (il) population derived from a cross between solanum lycopersicum 'moneyberg' and the wild species solanum chmielewskii revealed three ils with a pink fruit color. these lines had a homozygous s. chmielewskii introgression on the short arm of chromosome 1, consistent with the position of the y (yellow) mutation known to result in colorless epidermis, and hence pink-colored f ... | 2010 | 19906891 |
| genetic and physiological analysis of tomato fruit weight and composition: influence of carbon availability on qtl detection. | throughout tomato domestication, a large increase in fruit size was associated with a loss of dry matter and sugar contents. this study aims to dissect the contributions of genetic variation and the physiological processes underlying the relationships between fruit growth and the accumulation of dry matter and sugars. fruit quality traits and physiological parameters were measured on 20 introgression lines derived from the introgression of solanum chmielewskii into s. lycopersicum, under high (h ... | 2009 | 19179559 |
| fine mapping of quantitative trait loci for improved fruit characteristics from lycopersicon chmielewskii chromosome 1. | the near-isogenic line (nil) ta1150 contains a 56-cm introgression from lycopersicon chmielewskii chromosome 1 and has several interesting phenotypic characteristics including fruit with orange color, high levels of soluble solids, thick pericarp, small stem scars, and good firmness. a set of overlapping recombinant lines (subnils) was developed and field tested to fine map the quantitative trait loci (qtl) controlling these traits. the results indicated that the solids, pericarp thickness, and ... | 2003 | 12723039 |
| genes from lycopersicon chmielewskii affecting tomato quality during fruit ripening. | three chromosomal segments from the wild tomato, l. chmielewskii, introgressed into the l. esculentum genome have been previously mapped to the middle and terminal regions of chromosome 7 (7m, 7t respectively), and to the terminal region of chromosome 10 (10t). the present study was designed to investigate the physiological mechanisms controlled by the 7m and 7t segments on tomato soluble solids (ss) and ph, and their genetic regulation during fruit development. the effects of 7m and 7t were stu ... | 1995 | 24169841 |
| effects of the lycopersicon chmielewskii sucrose accumulator gene (sucr) on fruit yield and quality parameters following introgression into tomato. | a gene controlling fruit sucrose accumulation, sucr, was introgressed from the wild tomato species lycopersicon chmielewskii into the genetic background of a hexose-accumulating cultivated tomato, l. esculentum. during introgression, the size of the l. chmielewskii chromosomal segment containing sucr was reduced by selection for recombination between rflp markers for the sucr gene and flanking loci. the effects of sucr on soluble solids content, fruit size, yield and other fruit parameters were ... | 1995 | 24169782 |
| introgression into tomato (lycopersicon esculentum) of the l. chmielewskii sucrose accumulator gene (sucr) controlling fruit sugar composition. | high sucrose concentration in fruit of lycopersicon chmielewskii is governed by the recessive sucrose accumulator gene (sucr) that is situated in the pericentromeric region of chromosome 3. the sucr gene was introgressed into the genetic background of the hexose-accumulating cultivated tomato (l. esculentum cv 'hunt 100') by marker-assisted selection using tightly linked rflp markers and a tomato acid invertase cdna as probes for sucr. rflp mapping indicated that the segment containing sucr comp ... | 1995 | 24169781 |
| expression of acid invertase gene controls sugar composition in tomato (lycopersicon) fruit. | a wild tomato species, lycopersicon chmielewskii, accumulates high levels of soluble sugar in mature fruit and, unlike the domesticated tomato species, lycopersicon esculentum, accumulates sucrose rather than glucose and fructose. genetic and biochemical analyses of progeny resulting from a cross of l. chmielewskii with l. esculentum have previously indicated that the trait of sucrose accumulation is controlled by a single recessive gene and is associated with low levels of acid invertase protei ... | 1993 | 12231984 |
| evidence for the involvement of sucrose phosphate synthase in the pathway of sugar accumulation in sucrose-accumulating tomato fruits. | to better understand the mechanism of sugar unloading and sugar concentration in hexose- and sucrose-accumulating tomato fruits (lycopersicon chmielewskii and l. esculentum, respectively) and to determine the causes of the late accumulation of sucrose present in sucrose-accumulating tomato fruits, the assimilation of [(3)h](fructosyl)-sucrose was studied. key enzymes involved in carbohydrate metabolism were also assayed. the results demonstrated that the low level of sucrose present in young fru ... | 1992 | 16668903 |
| sucrose synthase in wild tomato, lycopersicon chmielewskii, and tomato fruit sink strength. | here it is reported that sucrose synthase can be readily measured in growing wild tomato fruits (lycopersicon chmielewskii) when suitable methods are adopted during fruit extraction. the enzyme also was present in fruit pericarp tissues, in seeds, and in flowers. to check for novel characteristics, the wild tomato fruit sucrose synthase was purified, by (nh(4))(2)so(4) fraction and chromatography with de-32, sephadex g-200, and pba-60, to one major band on sodium dodecyl sulfate-polyacrylamide g ... | 1992 | 16668741 |
| sink metabolism in tomato fruit : iv. genetic and biochemical analysis of sucrose accumulation. | fruit of domesticated tomato (lycopersicon esculentum) accumulate primarily glucose and fructose, whereas some wild tomato species, including lycopersicon chmielewskii, accumulate sucrose. genetic analysis of progeny resulting from a cross between l. chmielewskii and l. esculentum indicated that the sucrose-accumulating trait could be stably transferred and that the trait was controlled by the action of one or two recessive genes. biochemical analysis of progeny resulting from this cross indicat ... | 1991 | 16668087 |
| fine mapping of quantitative trait loci using selected overlapping recombinant chromosomes, in an interspecies cross of tomato. | quantitative trait loci (qtls) have been mapped to small intervals along the chromosomes of tomato (lycopersicon esculentum), by a method we call substitution mapping. the size of the interval to which a qtl can be mapped is determined primarily by the number and spacing of previously mapped genetic markers in the region surrounding the qtl. we demonstrate the method using tomato genotypes carrying chromosomal segments from lycopersicon chmielewskii, a wild relative of tomato with high soluble s ... | 1990 | 1968874 |
| acclimation of two tomato species to high atmospheric co(2): ii. ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase. | lycopersicon esculentum mill. cv vedettos and lycopersicon chmielewskii rick, la 1028, were exposed to two co(2) concentrations (330 or 900 microliters per liter) for 10 weeks. the elevated co(2) concentrations increased the initial ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activity of both species for the first 5 weeks of treatment but the difference did not persist during the last 5 weeks. the activity of mg(2+)-co(2)-activated rubisco was higher in 900 microliters per liter fo ... | 1989 | 16666953 |
| acclimation of two tomato species to high atmospheric co(2): i. sugar and starch concentrations. | lycopersicon esculentum mill. cv vedettos and lycopersicon chmielewskii rick, la 1028, were exposed to two co(2) concentrations (330 or 900 microliters per liter) for 10 weeks. tomato plants grown at 900 microliters per liter contained more starch and more sugars than the control. however, we found no significant accumulation of starch and sugars in the young leaves of l. esculentum exposed to high co(2). carbon exchange rates were significantly higher in co(2)-enriched plants for the first few ... | 1989 | 16666952 |
| identification of restriction fragment length polymorphisms linked to genes controlling soluble solids content in tomato fruit. | gene(s) conferring high soluble solids (ss) in tomato fruit had been backcrossed previously from a wild tomato species, lycopersicon chmielewskii la1028 (∼ 10% ss), into a l. esculentum cultivar, vf36 (∼ 5% ss), to derive a bc5s5 line, la1563, similar to 'vf 36' but with 7-8% ss. dnas from these lines and a tomato breeding line, h2038, were screened for restriction fragment length polymorphisms (rflps) using four restriction endonucleases and sixty clones chosen at random from a tomato cdna libr ... | 1987 | 24240994 |