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[study on isoenzymes of paragonimus iloktsuenensis and paragonimus westermani by isoelectric focusing].the isoenzyme patterns of paragonimus iloktsuenesis and p. westermani were studied by isoelectric focusing on polyacrylamide gels for pgm, mdh, hk, me, po and acp. interspecific differences were apparent as shown by the isoenzyme profiles of these enzymes examined. mdh was polymorphic with 2 recognizable phenotypes, designated common type and variant type respectively, in p. i. and with 3 phenotypes, designated mdh1, mdh2 and mdh1-2 respectively, in p. w. the result showed that the two species c ...19911959172
[elisa of rat sera infected with paragonimus iloktsuenensis].enzyme-linked immunosorbent assay (elisa) of paragonimiasis iloktsuenensis rat sera was performed using crude antigens of paragonimus iloktsuenensis (pia), p. westermani (pwa) and clonorchis sinensis (csa). three crude antigens (pia, pwa, csa) were prepared to saline homogenated supernatants of whole adult worms. infected rat sera were obtained biweekly from the albino rats fed 50-80 metacercariae of p. iloktsuenensis through gastric catheter. experimental groups were divided into 4 groups: gi ( ...19902099174
[effect of praziquantel treatment on pulmonary lesions of rats infected with paragonimus iloktsuenensis].an experimental pathological study was performed to observe the effect of praziquantel treatment on the pulmonary lesions of the rat lung fluke, paragonimus iloktsuenensis. the metacercariae were obtained from the freshwater crab, sesarma dehaani, and 40 rats (wistar) were fed each with 10 metacercariae. on 20 rats praziquantel treatment (100mg/kg/day x 5 days) was done at 5 weeks after the infection while remaining 20 rats were kept untreated for use as controls. the drug-treated rats and the u ...19892486830
[experimental infection of paragonimus iloktsuenensis to albino rats, dogs and cats].this study was performed to observe the susceptibility of dogs and cats as definitive hosts of paragonimus iloktsuenensis. the metacercariae of this fluke were obtained from sesarma dehaani collected at a focus near the mouth of sumjin river in november, 1986 and february, 1987. the larvae isolated from the crabs were introduced per os into 7 albino rats, 2 dogs and 3 cats. the adults were recovered from the experimental animals, and they were morphologically observed and measured. the results w ...19892486930
[infection status of sesarma dehaani collected from sumjin river delta with the metacercariae of paragonimus iloktsuenensis].this study was performed to observe the recent infection status of sesarma dehaani with the metacercariae of p. iloktsuenensis in the well-known enzootic focus, sumjin river delta. a total of 74 sesarma dehaani were collected from a focus near the mouth of the sumjin river in november, 1986 and february, 1987. the crabs were examined for p. iloktsuenensis metacercariae by the method of seo and kwak(1972). the metacercariae of p. iloktsuenensis were found in the liver of the crabs. among the 74 c ...19892486934
[tegumental ultrastructures of paragonimus iloktsuenensis according to the developmental stages].a scanning electron microscopic study was performed to observe the tegumental ultrastructures of paragonimus iloktsuenensis according to its developmental stages. the metacercariae were obtained from the liver of the brackish water crab, sesarma dehaani. juvenile and adult p. iloktsuenensis were recovered from the experimental rats on 2, 4 and 8 weeks after infection. the findings were summarized as follows: 1. the excysted metacercariae were characteristically gourd-shape, with their whole body ...19892487264
[scanning electron microscopy of the metacercaria of paragonimus iloktsuenensis]. 19873447785
genetic variability and differentiation of natural populations in three japanese lung flukes, paragonimus ohirai, paragonimus iloktsuenensis and paragonimus sadoensis (digenea: troglotrematidae).the genetic differences among 3 closely related species, paragonimus ohirai, paragonimus iloktsuenensis and paragonimus sadoensis, were assessed by electrophoretic analysis of allozymes. fifteen enzymes were analyzed using starch gel electrophoresis. seven of 18 loci were polymorphic. the most variable population (pos) was polymorphic at 6 of the loci (p = 0.333) with a heterozygosity h of 0.054. genetic distances between p. ohirai and p. iloktsuenensis, between p. ohirai and p. sadoensis, and b ...19863746563
evidence for metacercarial polymorphism in lung flukes, paragonimus ohirai and paragonimus iloktsuenensis.cross breeding experiments between 2 species of lung fluke, paragonimus ohirai and paragonimus iloktsuenensis, were carried out using metacercarial characteristics as distinguishing markers. all metacercariae of f1 obtained were identical to those of p. ohirai. in the f2 and bf1 of f1 x p. iloktsuenensis, both the p. ohirai and p. iloktsuenensis types of metacercariae appeared. in the bf1 of f1 x p. ohirai, however, only the p. ohirai type metacercariae were produced. no intermediate type betwee ...19854093814
[ultrastructural antigenic localization in paragonimus iloktsuenensis during developmental stage by immunogold labeling method].antigenic localization in paragonimus iloktsuenensis worm tissues (tegument, intestine and vitelline gland) in different developmental stages of 2 weeks, 3 weeks, 4 weeks, 5 weeks and 33 weeks from albino rats (sprague-dawley) infected with p. iloktsuenensis was observed by electron microscopy. these worm tissues of different developmental stage of p. iloktsuenensis was observed on electromicrograph by immunogold labeling method using p. iloktsuenensis infected rat serum of 10 weeks. antigenic l ...19958591015
molecular evidence for the synonymy of three species of paragonimus, p. ohirai miyazaki, 1939, p. iloktsuenensis chen, 1940 and p. sadoensis miyazaki et al., 1968the paragonimus ohirai group, named after p. ohirai miyazaki, 1939, consists of three nominal species. paragonimus iloktsuenensis chen, 1940 and p. sadoensis miyazaki et al., 1968, the other members of the group, were proposed primarily because of perceived differences in metacercarial morphology and/or host preferences. it has long been recognized that adults of the three were virtually indistinguishable. with the application of genetic techniques, it has become clear that the three forms can e ...19979445405
immunoelectron microscopic localization of partially purified antigens in adult paragonimus iloktsuenesis.an immunoelectron microscopy employing immunogold labeling method was performed to detect tissue origin of d1 fraction (d1a) among 5 antigenic protein fractions partially purified by deae-anion exchange chromatography from water-soluble crude antigen (piwa) of adult paragonimus iloktsuenensis. immune reactions of adult worm tissues with rabbit serum immunoglobulin immunized with crude antigen (pi-ig) and d1 antigen (d1-ig), as well as rat serum immunoglobulin infected with p. iloktsuenensis were ...200111441499
[studies on the lungfluke, paragonimus iloktsuenensis: vi. effect of prednisolone injection on the immune responses of albino rats]in order to understand the effect of prednisolone injection on the immune responses of albino rat against paragonimus iloktsuenensis infection, the differential leucocyte counts, appearance of immunoblast (large pyroninophilic cell, lpc) in the spleen and lungs in various experimental groups were observed in relation with the growth, maturation and migration sites of this rodent lungfluke. rats of 3 experimental groups (a series), each group consisted of 5 rats, were infected with 20 metacercari ...197612913442
[studies on the lungfluke, paragonimus iloktsuenensis: v. host tissue reactions in albino rats]in order to understand the tissue responses of albino rat host against paragonimus iloktsuenensis infection, the histopathological changes of the spleen and the lungs in 6 experimental groups of rats were observed in relation with the growth, maturation and migration of this lung fluke. rats of the experimental groups, each group consisted of 5 rats, were infected with the metacercariae of p. iloktsuenensis which were isolated from brackish water crab, sesarma dehaani, and were kept for 3 days, ...197612913445
studies on the lungfluke, paragonimus iloktsuenensis: iv. a mathematical analysis on metacercarial density of paragonimus iloktsuenensis in crab host, sesarma dehaani.mathematical models such as the negative binomial, poisson and polya-eggenberger distributions were applied to the observed data of the number of metacercariae of paragonimus iloktsuenensis in crab hosts, sesarma dehaani which were collected in hadong, south kyong-sang do, korea. from the above analysis, it was found that the pattern of density of metacercariae of this lungfluke among the crab hosts was well fitted to the negative binomial distribution, rather than to the poisson or polya-eggenb ...197512913464
the excretory system, particularly its flame cell pattern of paragonimus iloktsuenensis chen, 1940. 196013752879
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