herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in c4-fibre plants.the enrichment of nitrogen-fixing bacteria from the c4-fibre plants, spartina pectinata, miscanthus sinensis, miscanthus sacchariflorus and pennisetum purpureum, with nitrogen-free semi-solid media led to the isolation of herbaspirillum-like strains among other diazotrophic bacteria. on the basis of physiological properties, phylogenetic analysis comparing 16s rdna sequences and dna-dna hybridization experiments of chromosomal dna the new isolates could be grouped together in a new species with ...200111211253
defoliation effects on the community structure of arbuscular mycorrhizal fungi based on 18s rdna sequences.the effects of defoliation on arbuscular mycorrhizal (am) associations in the field were investigated in terms of the community structure of am fungi colonizing roots of grassland plants; the carbohydrate balance of the host plants was also determined. we focused on two plant species dominating japanese native grasslands: the grazing-intolerant species miscanthus sinensis and the grazing-tolerant species zoysia japonica. community structures of am fungi were determined from 18s rrna gene sequenc ...200414727168
improved pcr primers for the detection and identification of arbuscular mycorrhizal fungi.a set of pcr primers that should amplify all subgroups of arbuscular mycorrhizal fungi (amf, glomeromycota), but exclude sequences from other organisms, was designed to facilitate rapid detection and identification directly from field-grown plant roots. the small subunit rrna gene was targeted for the new primers (aml1 and aml2) because phylogenetic relationships among the glomeromycota are well understood for this gene. sequence comparisons indicate that the new primers should amplify all publi ...200818631176
bioethanol production from dedicated energy crops and residues in arkansas, usa.globally, one of the major technologic goals is to achieve cost-effective lignocellulosic ethanol production from biomass feedstocks. lignocellulosic biomass of four dedicated energy crops [giant reed (arundo donax l.), elephantgrass (pennisetum purpureum (schumach), miscanthus × giganteus (illinois clone), and (clone q42641) {hybrid of miscanthus sinensis anderss. and miscanthus sacchariflorus (maxim)}, hack. called giant miscanthus, and sugarcane clone us 84-1028 (saccharum l. spp. hybrid)] an ...201121086455
an improved plant leaf protein extraction method for high resolution two-dimensional polyacrylamide gel electrophoresis and comparative proteomics.we report here a simple and universally applicable protocol for extracting high quality proteins from plant leaf tissues. the protocol provides improved resolution and reproducibility of two-dimensional polyacrylamide gel electrophoresis (2-de) and reduces the time required to analyze samples. partitioning rubisco by polyethylene glycol (peg) fractionation provides clearer detection of low-abundance proteins. co-extraction of interfering substances increases the sample conductivity, which result ...201323072551
how cell wall complexity influences saccharification efficiency in miscanthus sinensis.the production of bioenergy from grasses has been developing quickly during the last decade, with miscanthus being among the most important choices for production of bioethanol. however, one of the key barriers to producing bioethanol is the lack of information about cell wall structure. cell walls are thought to display compositional differences that lead to emergence of a very high level of complexity, resulting in great diversity in cell wall architectures. in this work, a set of different te ...201525908240
production of hydrogen and volatile fatty acid by enterobacter sp. t4384 using organic waste a study of hydrogen-producing bacteria, strain t4384 was isolated from rice field samples in the republic of korea. the isolate was identified as enterobacter sp. t4384 by phylogenetic analysis of 16s rrna and rpob gene sequences. enterobacter sp. t4384 grew at a temperature range of 10-45 degrees c and at an initial ph range of 4.5-9.5. strain t4384 produced hydrogen at 0-6% nacl by using glucose, fructose, and mannose. in serum bottle cultures using a complete medium, enterobacter sp. t4384 ...201323412061
early-stage changes in natural (13)c and (15)n abundance and nutrient dynamics during different litter decomposition.decomposition, nutrient, and isotopic (δ(13)c and δ(15)n) dynamics during 1 year were studied for leaf and twig litters of pinus densiflora, castanea crenata, erigeron annuus, and miscanthus sinensis growing on a highly weathered soil with constrained nutrient supply using litterbags in a cool temperate region of south korea. decay constant (k/year) ranged from 0.58 to 1.29/year, and mass loss ranged from 22.36 to 58.43 % among litter types. the results demonstrate that mass loss and nutrient dy ...201626915037
wild plants, andropogon virginicus l. and miscanthus sinensis anders, are tolerant to multiple stresses including aluminum, heavy metals and oxidative isolate high-tolerant plants against aluminum (al), heavy metals and/or oxidative stresses as a final goal, screening of al tolerant plants from a collection of 49 wild plants was first of all performed in this study. andropogon virginicus l. and miscanthus sinensis anders showed high al tolerant phenotypes (more than 35% values in both relative root growth and germination frequency even under 900 microm al concentration) in our screening. al tolerance mechanisms in these two plants were char ...200818204843
ornamental plants for micropollutant removal in wetland systems.the objective of this paper was to evaluate the efficiency of micropollutant removal, such as cu, zn, carbamazepine, and linear alkylbenzene sulfonates (las), through the use of a subsurface vertical flow constructed wetland system with ornamental plants. zantedeschia aethiopica, canna indica, carex hirta, miscanthus sinensis, and phragmites australis were selected and planted in lysimeters filled up with gravel. the lysimeters were completely saturated with synthetic wastewater (n 280 mg l(-1), ...201524798922
engineering saccharomyces pastorianus for the co-utilisation of xylose and cellulose from biomass.lignocellulosic biomass is a viable source of renewable energy for bioethanol production. for the efficient conversion of biomass into bioethanol, it is essential that sugars from both the cellulose and hemicellulose fractions of lignocellulose be utilised.201525928878
[digestive enzymes in the proboscis of eulalia viridis (müller) (phyllococidae) and of glycera convouuta (keferstein) (glyceridae)--erratic polychaetous annelides]. 20144180312
inhibitory mechanisms of glycoprotein fraction derived from miscanthus sinensis for the immediate phase response of an ige-mediated cutaneous reaction.we investigated the inhibitory effect of the glycoprotein fraction (fraction 2) extracted from miscanthus sinensi andersson (m. sinensis) on biphasic cutaneous reactions in mice passively sensitized with ige. biphasic skin reactions with peak responses at 1 (ipr, immediate phase reaction) and 24 h (lpr, late phase reaction) were caused by passive sensitization with an anti-dinitrophenol ige monoclonal antibody (anti-dnp ige mab) followed by an epicutaneous challenge of 0.1% dinitrofluorobenzene ...19999989657
inhibitory effect of the water extract of spikes of miscanthus sinensis on ige formation in mice.the effects of four different plant extracts of the spikes of miscanthus sinensis, phragmites communis, and imperata cylindrica var. major, and of the spikelets of coix lachryma-jobi on ige antibody formation were investigated in mice. the ige antibody titer was tested by the passive cutaneous anaphylaxis (pca) method in rats. of these, the water extract of m. sinensis showed an appreciable inhibitory effect on ige formation. furthermore, an undialyzable fraction of the water extract with a rela ...19938302952
dart-based characterisation of genetic diversity in a miscanthus collection from poland.analysis of 180 accessions of miscanthus using a dart platform revealed high diversity. the phylogenetic analysis revealed that m. × giganteus accessions fall into two genetically distinct groups. miscanthus is a genus of perennial rhizomatous grasses that has emerged in last 20 years as a feedstock for bioenergy and biofuel production. currently, the most widely used accession for bioenergy purposes is miscanthus × giganteus, a sterile triploid hybrid between miscanthus sinensis and miscanthus ...201526040407
revision of ishigakia uchida (hymenoptera: ichneumonidae: acaenitinae) from japan, with a new species having a close relative in south africa.two japanese species of the genus ishigakia uchida, 1928, i.e., the type species from ryukyus, i. exetasea uchida, and a new species from honshu, i. albitarsa ito & maeto sp. nov., are (re-)described. antennal tapping and oviposition behavior of female i. exetasea was observed on the stalks of japanese silvergrass miscanthus sinensis. the new species, i. albitarsa, has (presumably) close relatives from mainland china and, disjunctely, from south africa. a key to the world species of ishigakia is ...201627395711
[bioaccumulation of heavy metals by the dominant plants growing in huayuan manganese and lead/zinc mineland, xiangxi].heavy metal pollution is a major environmental problem of mine wasteland. finding out the tolerant plants, which can adapt to the local climate and the soil conditions, is the premise of vegetation restoration. an extensive vegetation survey and sampling were conducted in huayuan mn and pb/zn mineland, 76 species belonging to 69 genera and 39 families were recorded. the main dominant species and their associated soils were determined for heavy metal concentrations. the results showed that soil p ...201222946193
miscanthus as cellulosic biomass for bioethanol production.the members of the genus miscanthus are potential feedstocks for biofuels because of the promising high yields of biomass per unit of planted area. this review addresses species, cultivation, and lignocellulose composition of miscanthus, as well as pretreatment and enzyme saccharification of miscanthus biomass for ethanol fermentation. the average cellulose contents in dried biomass of miscanthus floridulus, miscanthus sinensis, miscanthus sacchariflorus, and miscanthus × giganteus (m × g) are 3 ...201526013948
discovery of natural miscanthus (poaceae) triploid plants in sympatric populations of miscanthus sacchariflorus and miscanthus sinensis in southern japan.• premise of the study: looming petroleum shortages and projected negative impacts of human-induced climate change may be partly alleviated by the development and use of bioenergy feedstock crops. miscanthus ×giganteus, a highly productive sterile triploid hybrid grass that was discovered in japan several decades ago, has considerable potential as an alternative source of energy. given the risks, however, involved in the reliance upon production of one clone of this hybrid, which is a natural cr ...201121613094
the parapatric distribution and contact zone of two forms showing different male-to-male aggressiveness in a social spider mite, stigmaeopsis miscanthi (acari: tetranychidae).two forms showing different male-to-male aggressiveness, different male morphologies and different diapause attributes are known in stigmaeopsis miscanthi (saito), a social spider mite infesting chinese silver grass (miscanthus sinensis anderss). reproductive isolation exists between the forms, although it is not always complete, and the details of their distributional patterns are unknown, but expected to be parapatric. we searched for the contact zone at mt. unzen (nagasaki pref., japan) where ...200818392941
novel endophytic nitrogen-fixing clostridia from the grass miscanthus sinensis as revealed by terminal restriction fragment length polymorphism analysis.anaerobic nitrogen-fixing consortia consisting of n2-fixing clostridia and diverse nondiazotrophic bacteria were previously isolated from various gramineous plants (k. minamisawa, k. nishioka, t. miyaki, b. ye, t. miyamoto, m. you, a. saito, m. saito, w. barraquio, n. teaumroong, t. sein, and t. tadashi, appl. environ. microbiol. 70:3096-3102, 2004). for this work, clostridial populations and their phylogenetic structures in a stand of the grass miscanthus sinensis in japan were assessed by a 16 ...200415528521
shoot population dynamics of persisting clones of miscanthus sinensis in the warm-temperate region of japan.the population dynamics of annual shoots in four clonal patches of miscanthus sinensis was surveyed in terms of clone persistence. over a study period of 3 years, a stable net shoot number was attained through a balanced replacement of old shoots by tillering 2-3 times a year. the birth rate was significantly correlated with the average monthly temperature. this suggests that a warm climate advances the date of tillerings, and shoots become taller as they emerge earlier. five cohorts were identi ...200314523617
within- and between-individual length heterogeneity of the rdna-igs in miscanthus sinensis var. glaber (poaceae): phylogenetic analyses.the variability in the intergenic spacer (igs) region between 17s and 25s rrna genes of ribosomal dna (rdna) gene family was surveyed in miscanthus sinensis var. glaber. length heterogeneity, with sizes from 1782 to 2212 base pairs, of the igs resulted from the variation of copy numbers of the a and b subrepeats. these repeated elements were located upstream of the presumptive polymeraseii promoter, which was the region corresponding to the nontranscribed spacer (nts). length heterogeneity was d ...199910659774
effect of spikelets of miscanthus sinensis on ige-mediated biphasic cutaneous reaction in mice.the effect of spikelets of miscanthus sinensis andersson (m. sinensis) on ige-mediated biphasic cutaneous reactions was investigated in balb/c mice. mice were passively sensitized by an intravenous (i.v.) injection of monoclonal antidinitrophenol ige antibody (anti-dnp ige mab), or actively by an intraperitoneal (i.p.) injection of dnp-derivatized ovalbumin (dnp-ova) plus aluminium hydroxide gel (alum) as an adjuvant. skin reactions were elicited by an epicutaneous challenge of dinitrofluorobenz ...19989491762
phylogenetic analysis of chloroplast restriction enzyme site mutations in the saccharinae griseb. subtribe of the andropogoneae dumort. tribe.chloroplast (cp) dna from 32 genotypes representing eight genera and 19 species from the andropogoneae tribe was analyzed using 15 restriction enzymes and southern hybridization with 12 cpdna probes that span the complete rice chloroplast genome. six of the genera, saccharum, miscanthus, erianthus, narenga, eccoilopus, and sclerostachya, are part of the saccharinae subtribe, whereas the other two, zea and sorghum, were used as outgroups. narenga, miscanthus, erianthus, and sclerostachya are pres ...199424190471
cloning and characterization of a new β-glucosidase from a metagenomic library of rumen of cattle feeding with miscanthus sinensis.the study on the second generation bio-fuel is a hot area of current research of renewable energy. among series of key points in this area, the role of β-glucosidase in the degradation of intermediate gluco-oligosaccharides limits the rate of the complete saccharification of lignocellulose.201425274487
root ethylene signalling is involved in miscanthus sinensis growth promotion by the bacterial endophyte herbaspirillum frisingense gsf30(t).the bacterial endophyte herbaspirillum frisingense gsf30(t) is a colonizer of several grasses grown in temperate climates, including the highly nitrogen-efficient perennial energy grass miscanthus. inoculation of miscanthus sinensis seedlings with h. frisingense promoted root and shoot growth but had only a minor impact on nutrient concentrations. the bacterium affected the root architecture and increased fine-root structures. although h. frisingense has the genetic requirements to fix nitrogen, ...201324043849
effects of inclusion levels of pelleted silvergrass (miscanthus sinensis andress.) in the diet on digestibility, chewing activity, ruminal fermentation and blood metabolites in breeding japanese black examine the effects of inclusion levels of pelleted silvergrass (ps) in the diet on digestibility, ruminal fermentation and nutrient status of breeding japanese black cows, four cows were allotted to a 4 × 4 latin square design experiment. treatments were control fed a diet consisting of 89.4% sudangrass hay and 10.6% soybean meal on a dry matter (dm) basis, and ps18, ps27 and ps45 fed the diet replaced with 18%, 27% and 45% of control with ps, respectively. the total digestible nutrients (td ...201727444406
endophytic root colonization of gramineous plants by herbaspirillum frisingense.herbaspirillum frisingense is a diazotrophic betaproteobacterium isolated from c4-energy plants, for example miscanthus sinensis. to demonstrate endophytic colonization unequivocally, immunological labeling techniques using monospecific polyclonal antibodies against two h. frisingense strains and green fluorescent protein (gfp)-fluorescence tagging were applied. the polyclonal antibodies enabled specific in situ identification and very detailed localization of h. frisingense isolates mb11 and gs ...200818761671
three-dimensional surface reconstruction and in situ site-specific cutting of the teliospores of puccinia miscanthi causing leaf rust of the biomass plant miscanthus sinensis.the rust fungus puccinia miscanthi on the naturally infected leaves of eulalia grass were investigated by light and electron microscopy. many black telia were present on both the adaxial and abaxial leaf surfaces. the telia were often confluent, protruding distinctly from the leaf surface, and had numerous teliospores. light microscopy revealed that the teliospores were club-like in shape (clavate), chestnut brown, and produced pedicels from one end. they were measured to be approximately 45 μm ...201525855600
ultrastructure of the rust fungus puccinia miscanthi in the teliospore stage interacting with the biofuel plant miscanthus sinensis.interaction of the the rust fungus puccinia miscanthi with the biofuel plant miscanthus sinensis during the teliospore phase was investigated by light and electron microscopy. p. miscanthi telia were oval-shaped and present on both the adaxial and abaxial leaf surfaces. teliospores were brown, one-septate (two-celled), and had pedicels attached to one end. transmission electron microscopy revealed numerous electron-translucent lipid globules in the cytoplasm of teliospores. extensive cell wall d ...201526361478
hormone independent regeneration in eulalia viridis (polychaeta-phyllodocidae). 20051169185
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