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partially consolidated bioprocessing of mixed lignocellulosic feedstocks for ethanol production.rapid urbanization and industrialization have accelerated the energy demand which cannot be met by decreasing fossil fuels thereby substantiate the need for lignocellulosic ethanol. the present study is one such attempt towards bioethanol production in an eco-friendly manner using enzymes in which a mixture of lignocellulosic biomass namely, ricinus communis, saccharum officinarum (tops) and saccharum spontaneum were taken as a substrate. the mixed biomass was processed through partially consoli ...201728898853
species composition, distribution, life forms and folk nomenclature of forest and common land plants of western chitwan, nepal.this paper enumerates 349 plant species belonging to 77 families of vascular plants collected in the winter seasons of 1996 and 2000 by the flora teams of the population and ecology research laboratory, nepal. of the total species, 249 species belong to dicotyledons, 87 species to monocotyledons and 13 species to pteridophytes. among the families, dicotyledons contributed the highest number of families (55 in number) followed by monocotyledons and pteridophytes. in the study areas, species compo ...200522962539
use of saccharum spontaneum (wild sugarcane) as biomaterial for cell immobilization and modulated ethanol production by thermotolerant saccharomyces cerevisiae vs3.saccharum spontaneum is a wasteland weed consists of 45.10+/-0.35% cellulose and 22.75+/-0.28% of hemicellulose on dry solid (ds) basis. aqueous ammonia delignified s. spontaneum yielded total reducing sugars, 53.91+/-0.44 g/l (539.10+/-0.55 mg/g of substrate) with a hydrolytic efficiency of 77.85+/-0.45%. the enzymes required for hydrolysis were prepared from culture supernatants of aspergillus oryzae mtcc 1846. a maximum of 0.85+/-0.07 iu/ml of filter paperase (fpase), 1.25+/-0.04 iu/ml of car ...200919114303
degradation of textile dyes mediated by plant peroxidases.the peroxidase enzyme from the plants ipomea palmata (1.003 iu/g of leaf) and saccharum spontaneum (3.6 iu/g of leaf) can be used as an alternative to the commercial source of horseradish and soybean peroxidase enzyme for the decolorization of textile dyes, mainly azo dyes. eight textiles dyes currently used by the industry and seven other dyes were selected for decolorization studies at 25-200 mg/l levels using these plant enzymes. the enzymes were purified prior to use by ammonium sulfate prec ...200612396133
process optimisation for the biosynthesis of cellulase by bacillus pc-bc6 and its mutant derivative bacillus n3 using submerged fermentation.this study deals with optimisation of cultural conditions for enhanced production of cellulase by bacillus pc-bc6 and its mutant derivative bacillus n3. influence of different variables including incubation time, temperature, inoculum size, ph, nitrogen sources and metal ions has been studied. the optimum conditions for cellulase production were incubation period of 72 h, inoculum size 4% incubation temperature 37°c, ph 7, 0.25% ammonium sulphate, 0.2% peptone as inorganic and organic nitrogen s ...201525421057
phytodiversity on fly ash deposits: evaluation of naturally colonized species for sustainable phytorestoration.proliferation of fly ash (fa) deposits and its toxicity have become a global concern, which contaminate the ecosystems of our earth. in this regard, identification of potential plant species for fa deposits' restoration is the main concern. keeping this view in mind, the present study was conducted to identify potential plant species naturally growing on fa deposits for the restoration purposes. six intensive surveys were made during 2010-2014 to collect naturally growing plant species during di ...201525209541
immunoglobulin e-binding reactivities of natural pollen grain extracts from selected grass species in the philippines.pollen grains have been reported to be present in the philippine atmosphere but studies regarding their allergenicity are limited.201222701864
bioconversion potential of trichoderma viride hn1 cellulase for a lignocellulosic biomass saccharum spontaneum.the industrialisation of lignocellulose conversion is impeded by expensive cellulase enzymes required for saccharification in bioethanol production. current research undertakes cellulase production from pretreated saccharum spontaneum through trichoderma viride hn1 under submerged fermentation conditions. pretreatment of substrate with 2% naoh resulted in 88% delignification. maximum cellulase production (2603 ± 16.39 u/ml/min carboxymethyl cellulase and 1393 ± 25.55 u/ml/min fpase) was achieved ...201525346145
a molecular approach to unraveling the genetics of sugarcane, a complex polyploid of the andropogoneae tribe.modern sugarcane varieties are complex aneuploids and typically have chromosome numbers in the 100-125 range with about 5-10% of them contributed by wild relatives, mainly saccharum spontaneum, and the rest by s. officinarum. this particular genomic constitution was found favorable for mapping the s. spontaneum genome, using maize as a diploid reference for comparison. we conducted an analysis of 32 individuals derived from the selfing of variety sp 701006 using four isozymes and 53 maize probes ...199418470072
a bac library of the sp80-3280 sugarcane variety (saccharum sp.) and its inferred microsynteny with the sorghum genome.sugarcane breeding has significantly progressed in the last 30 years, but achieving additional yield gains has been difficult because of the constraints imposed by the complex ploidy of this crop. sugarcane cultivars are interspecific hybrids between saccharum officinarum and saccharum spontaneum. s. officinarum is an octoploid with 2n = 80 chromosomes while s. spontaneum has 2n = 40 to 128 chromosomes and ploidy varying from 5 to 16. the hybrid genome is composed of 70-80% s. officinaram and 5- ...201222524198
using quantitative pcr with retrotransposon-based insertion polymorphisms as markers in sugarcane.sugarcane is the main source of the world's sugar and is becoming increasingly important as a source of biofuel. the highly polyploid and heterozygous nature of the sugarcane genome has meant that characterization of the genome has lagged behind that of other important crops. here we developed a method using a combination of quantitative pcr with a transposable marker system to score the relative number of alleles with a transposable element (te) present at a particular locus. we screened two ge ...201526093024
cold responsive gene expression profiling of sugarcane and saccharum spontaneum with functional analysis of a cold inducible saccharum homolog of nod26-like intrinsic protein to salt and water stress.transcriptome analysis of sugarcane hybrid cp72-1210 (cold susceptible) and saccharum spontaneum tus05-05 (cold tolerant) using sugarcane assembled sequences (sas) from sucest-fun database showed that a total of 35,340 and 34,698 sas genes, respectively, were expressed before and after chilling stress. the analysis revealed that more than 600 genes are differentially expressed in each genotype after chilling stress. blast2go annotation revealed that the major difference in gene expression profil ...201525938773
phylogenetic analysis of different ploidy saccharum spontaneum based on rdna-its sequences.saccharum spontaneum l. is a crucial wild parent of modern sugarcane cultivars whose ploidy clones have been utilized successfully in improving the stress resistance and yield related traits of sugarcane cultivars. to establish knowledge regarding the genetic variances and evolutional relationships of ploidy clones of saccharum spontaneum collected in china, the rdna-its sequences of 62 ploidy clones including octaploid clones (2n = 64), nonaploid clones (2n = 72), decaploid clones (2n = 80), an ...201626986847
isolation and characterization of centromeric repetitive dna sequences in saccharum spontaneum.sugarcane (saccharum hybrids spp.) is the most important sugar crop that accounts for ~75% of the world's sugar production. recently, a whole-genome sequencing project was launched on the wild species s. spontaneum. to obtain information on the dna composition of the repeat-enriched region of the centromere, we conducted a genome-wide analysis of the dna sequences associated with cenh3 (a mutant of histone h3 located in eukaryote centromeres) using chromatin immunoprecipitation followed by seque ...201728134354
comparative structural analysis of bru1 region homeologs in saccharum spontaneum and s. officinarum.sugarcane is a major sugar and biofuel crop, but genomic research and molecular breeding have lagged behind other major crops due to the complexity of auto-allopolyploid genomes. sugarcane cultivars are frequently aneuploid with chromosome number ranging from 100 to 130, consisting of 70-80 % s. officinarum, 10-20 % s. spontaneum, and 10 % recombinants between these two species. analysis of a genomic region in the progenitor autoploid genomes of sugarcane hybrid cultivars will reveal the nature ...201627287040
corrigendum to "characterization and evaluation of methylmethacrylate-acetylated saccharum spontaneum l. graft copolymers prepared under microwave'' [carbohydr. polym. 78/4 (november (17)) (2009) 987-996]. 201727842851
fungal planet description sheets: 214-280.novel species of microfungi described in the present study include the following from south africa: cercosporella dolichandrae from dolichandra unguiscati, seiridium podocarpi from podocarpus latifolius, pseudocercospora parapseudarthriae from pseudarthria hookeri, neodevriesia coryneliae from corynelia uberata on leaves of afrocarpus falcatus, ramichloridium eucleae from euclea undulata and stachybotrys aloeticola from aloe sp. (south africa), as novel member of the stachybotriaceae fam. nov. s ...201425264390
truncated ubiquitin 5' regulatory region from erianthus arundinaceus drives enhanced transgene expression in heterologous systems.characterization of novel plant gene promoters underpins the development of transgenic crop plants. here, we report a novel 5' regulatory sequence (eriubi d7) of the ubiquitin gene from erianthus arundinaceus, a wild relative of sugarcane resistant to many biotic and abiotic stresses. a 3.2-kb regulatory sequence of ubiquitin gene was isolated through random amplification of genomic ends technique and characterized in rice, tobacco, and sugarcane. in silico analysis revealed that the regulatory ...201525985900
genetic variability among the chloroplast genomes of sugarcane (saccharum spp) and its wild progenitor species saccharum spontaneum l.a striking characteristic of modern sugarcane is that all sugarcane cultivars (saccharum spp) share a common cytoplasm from s. officinarum. to explore the potential value of s. spontaneum cytoplasm, new saccharum hybrids with an s. spontaneum cytoplasm were developed at the united states department of agriculture-agricultural research service, sugarcane research laboratory, through a combination of conventional and molecular breeding approaches. in this study, we analyzed the genetic variability ...201424615073
herbal medicines used in the treatment of diabetes mellitus in arunachal himalaya, northeast, india.medicinal plants have played an important role in treating and preventing a variety of diseases throughout the world. khampti tribal people living in the far-flung lohit district of the eastern arunachal himalaya, india still depend on medicinal plants and most of them have a general knowledge of medicinal plants which are used for treating a variety of ailments. this survey was undertaken in lohit district in order to inventory the medicinal plants used in folk medicine to treat diabetes mellit ...201222433536
bioconversion of saccharum spontaneum (wild sugarcane) hemicellulosic hydrolysate into ethanol by mono and co-cultures of pichia stipitis ncim3498 and thermotolerant saccharomyces cerevisiae-vs(3).the lignocellulosic biomass is a low-cost renewable resource for eco-benign liquid fuel 'ethanol'. to resolve the hydrolysis of mixed sugars in lignocellulosic substrate saccharum spontaneum, the microbial co-cultures of pichia stipitis ncim 3498 and thermotolerant saccharomyces cerevisiae-vs(3) were analyzed for efficient bioconversion of mixed sugars into ethanol. among the hydrolysis conditions, the acid hydrolysis released maximum sugars along with furans, phenolics and acetic acid. the acid ...201021185411
bioconversion of novel substrate saccharum spontaneum, a weedy material, into ethanol by pichia stipitis ncim3498.rising fuel prices and environmental issues have paved the way for the exploration of cellulosic ethanol. however, challenges involving substrate hydrolysis and cost-effectiveness still limit the efficient bioconversion and utilization of cellulosic ethanol. we aimed to evaluate a cheaper and abundantly available wild sugarcane variety, saccharum spontaneum, as the raw substrate for bioconversion of ethanol by pichia stipitis ncim3498. three different strategies for substrate hydrolysis using ac ...201120822899
second generation bioethanol production from saccharum spontaneum l. ssp. aegyptiacum (willd.) hack.saccharum (saccharum spontaneum l. ssp. aegyptiacum (willd.) hack.), is a rapidly growing, wide ranging high-yield perennial, suitable for second generation bioethanol production. this study evaluated oxalic acid as a pretreatment for bioconversion. overall sugar yields, sugar degradation products, enzymatic glucan hydrolysis and ethanol production were studied as effects of temperature (150-190 degrees c), reaction time (10-40 min) and oxalic acid concentration 2-8% (w/w). time and temperature ...201020194020
comparative analysis of miscanthus and saccharum reveals a shared whole-genome duplication but different evolutionary fates.multiple polyploidizations with divergent consequences in the grass subtribe saccharinae provide a singular opportunity to study in situ adaptation of a genome to the duplicated state, heretofore known primarily from paleogenomics. we show that allopolyploidy in a common miscanthus-saccharum ancestor ∼3.8 to 4.6 million years ago closely coincides in time with their divergence from the sorghum lineage. subsequent saccharum-specific autopolyploidy may have created pseudo-paralogous chromosome gro ...201424963058
full-length enriched cdna libraries and orfeome analysis of sugarcane hybrid and ancestor genotypes.sugarcane is a major crop used for food and bioenergy production. modern cultivars are hybrids derived from crosses between saccharum officinarum and saccharum spontaneum. hybrid cultivars combine favorable characteristics from ancestral species and contain a genome that is highly polyploid and aneuploid, containing 100-130 chromosomes. these complex genomes represent a huge challenge for molecular studies and for the development of biotechnological tools that can facilitate sugarcane improvemen ...201425222706
analysis of three sugarcane homo/homeologous regions suggests independent polyploidization events of saccharum officinarum and saccharum spontaneum.whole genome duplication has played an important role in plant evolution and diversification. sugarcane is an important crop with a complex hybrid polyploid genome, for which the process of adaptation to polyploidy is still poorly understood. in order to improve our knowledge about sugarcane genome evolution and the homo/homeologous gene expression balance, we sequenced and analyzed 27 bacs (bacterial artificial chromosome) of sugarcane r570 cultivar, containing the putative single-copy genes lf ...201728082603
simultaneous cellulase production, saccharification and detoxification using dilute acid hydrolysate of s. spontaneum with trichoderma reesei ncim 992 and aspergillus niger.bioethanol production from lignocellulosic materials has several limitations. one aspect is the high production cost of cellulases used for saccharification of substrate and inhibition of fermenting yeast due to inhibitors released in acid hydrolysis. in the present work we have made an attempt to achieve simultaneous cellulases production, saccharification and detoxification using dilute acid hydrolysate of saccharum spontaneum with and without addition of nutrients, supplemented with acid hydr ...201123729891
cu-resistant kocuria sp. crb15: a potential pgpr isolated from the dry tailing of rakha copper mine.rhizobacteria may enhance biomass production and heavy metal tolerance of plants under stress conditions. the present study was carried out for isolation of metal-resistant bacteria that can be further utilized for phytoremediation process. a potential metal-resistant strain crb15 was isolated from rhizospheric region of saccharum spontaneum that was found to be resistant against cu (6.29 mm), zn (3.25 mm), pb (1.5 mm), ni (1.25 mm), and cd (0.25 mm). sem analysis was performed for evaluation of ...201728593515
de novo sequencing and transcriptome analysis of a low temperature tolerant saccharum spontaneum clone ind 00-1037.saccharum spontaneum l., a wild relative of sugarcane, is known for its adaptability to environmental stresses, particularly cold stress. in the present study, an attempt was made for transcriptome profiling of the low temperature (10°c) tolerant s. spontaneum clone ind 00-1037 collected from high altitude regions of arunachal pradesh, north eastern india. the illumina nextseq500 platform yielded a total of 47.63 and 48.18 million reads corresponding to 4.7 and 4.8 gigabase pairs (gb) of process ...201627269250
mechanistic investigation in ultrasound induced enhancement of enzymatic hydrolysis of invasive biomass species.this study has assessed four invasive weeds, viz. saccharum spontaneum (ss), mikania micrantha (mm), lantana camara (lc) and eichhornia crassipes (ec) for enzymatic hydrolysis prior to bioalcohol fermentation. enzymatic hydrolysis of pretreated biomasses of weeds has been conducted with mechanical agitation and sonication under constant (non-optimum) conditions. profiles of total reducible sugar release have been fitted to hch-1 model of enzymatic hydrolysis using genetic algorithm. trends in pa ...201626898160
restoring ecosystem services to littoral zones of rivers in the urban core of chongqing, china.two examples of the creation of naturalized areas in the littoral zone of the three gorges reservoir in the urban core of chongqing city, china, are described. the areas were created for the purpose of restoring ecological functions and services. plants were selected based on surveys of natural wetland vegetation in the region, and experiments were conducted to discover the capacity of species of interest to survive the sometimes extreme hydrological regimes at the sites. novel methods were deve ...201525907628
random mutagenesis and media optimisation for hyperproduction of cellulase from bacillus species using proximally analysed saccharum spontaneum in submerged fermentation.this study deals with the isolation of novel mutant of bacillus and optimisation of media for the hyperproduction of cellulase. cellulase-producing bacillus pc-bc6 was subjected to physical and chemical mutagenesis to enhance the cellulolytic potential. later, mutagenesis isolates were screened both qualitatively and quantitatively. among all the tested isolates, bacillus n3 yielded maximum (cmcase 1250 iu/ml/min and fpase 629 iu/ml/min) activity. the bacillus n3 strain exhibited 1.7-fold more e ...201525142026
inference of subgenomic origin of bacs in an interspecific hybrid sugarcane cultivar by overlapping oligonucleotide hybridizations.sugarcane (saccharum spp.) breeders in the early 20th century made remarkable progress in increasing yield and disease resistance by crossing saccharum spontaneum l., a wild relative, to saccharum officinarum l., a traditional cultivar. modern sugarcane cultivars have approximately 71%-83% of their chromosomes originating from s. officinarum, approximately 10%-21% from s. spontaneum, and approximately 2%-13% recombinant or translocated chromosomes. in the present work, c(0)t-based cloning and se ...201121883018
sugarcane genome sequencing by methylation filtration provides tools for genomic research in the genus saccharum.many economically important crops have large and complex genomes that hamper their sequencing by standard methods such as whole genome shotgun (wgs). large tracts of methylated repeats occur in plant genomes that are interspersed by hypomethylated gene-rich regions. gene-enrichment strategies based on methylation profiles offer an alternative to sequencing repetitive genomes. here, we have applied methyl filtration with mcrbc endonuclease digestion to enrich for euchromatic regions in the sugarc ...201424773339
genetic diversity of saccharum spontaneum from geographical regions of china assessed by simple sequence repeats.saccharum spontaneum is the most variable wild relative of sugarcane with potential for use in sugarcane improvement programs. in order to help preserve and exploit this species, 152 accessions from eight major geographical regions in china, including hainan, guangdong, guangxi, yunnan, sichuan, guizhou, fujian, and jiangxi provinces, were investigated by analyzing 20 simple sequence repeats (ssrs), including 11 genomic ssrs (gssrs) and nine ssrs developed from expressed sequence tags (est-ssrs) ...201324338385
research-artile agricultural performance and genetic parameters for yield-related traits of sugar- and energy cane families derived from planned crosses.the forecast of a growing energy demand in the coming years has aroused particular interest in biomass for energy cogeneration, to diversify the energy matrix by using clean and renewable sources. to meet the new demands of the sugarcane industry, this study evaluated the agronomic performance and estimated genetic parameters for yield traits in sugar- and energy cane families derived from planned crosses. the cane families were assessed in the northwest of the state of paraná, county of paranav ...201728973752
molecular insights into the origin of the brown rust resistance gene bru1 among saccharum species.analysis of 387 sugarcane clones using bru 1 diagnostic markers revealed two possible sources of bru 1 in chinese cultivars: one from saccharum spontaneum and another from saccharum robustum of new guinea. sugarcane brown rust (sbr) is an important fungal disease in many sugarcane production areas around the world, and can cause considerable yield losses in susceptible sugarcane cultivars. one major sbr resistance gene, named bru1, initially identified from cultivar r570, was shown to be a major ...201728821913
supply and demand: sink regulation of sugar accumulation in sugarcane.sugarcane (saccharum spp. hybrids) accumulates sucrose to high concentrations and, as a result, has been the focus of extensive research into the biochemistry and physiology of sucrose accumulation. despite this, the relationship between source leaf photosynthetic activity and sucrose accumulation in the culm sink is not well understood. the observations that photosynthetic activity declines during culm maturation in commercial cultivars and that high-sucrose-accumulating noble ancestral genotyp ...200919050062
comparative analysis of qtls affecting plant height and flowering among closely-related diploid and polyploid genomes.quantitative trait loci (qtls) affecting plant height and flowering were studied in the two saccharum species from which modern sugarcane cultivars are derived. two segregating populations derived from interspecific crosses between saccharum officinarum and saccharum spontaneum were genotyped with 735 dna markers. among the 65 significant associations found between these two traits and dna markers, 35 of the loci were linked to sugarcane genetic maps and 30 were unlinked dna markers. twenty-one ...200212416611
identification of cold-responsive genes in energycane for their use in genetic diversity analysis and future functional marker development.breeding for cold tolerance in sugarcane will allow its cultivation as a dedicated biomass crop in cold environments. development of functional markers to facilitate marker-assisted breeding requires identification of cold stress tolerance genes. using suppression subtractive hybridization, 465 cold-responsive genes were isolated from the cold-tolerant energycane ho02-144. predicted gene interactions network indicated several associated pathways that may coordinately regulate cold tolerance resp ...201323987817
detection of rdna sites in sugarcane by fish.hybridization sites of an rdna probe coding for the 5.8s, 18s and 26s genes were detected on the chromosomes of sugarcane and a related genus, erianthus, using fluorescence in situ hybridization. one unpaired and five paired hybridization sites were detected in a saccharum spp. hybrid. a first introgression hybrid (i1) between saccharum officinarum and saccharum spontaneum had seven pairs of hybridization sites. a clone of erianthus arundinaceus showed six hybridization sites in somatic tissue.19958528591
chromosome assortment in saccharum.recent work has revealed random chromosome pairing and assortment in saccharum spontaneum l., the most widely distributed, and morphologically and cytologically variable of the species of saccharum. this conclusion was based on the analysis of a segregating population from across between s. spontaneum 'ses 208' and a spontaneously-doubled haploid of itself, derived from anther culture. to determine whether polysomic inheritance is common in saccharum and whether it is observed in a typical bipar ...199424178110
srap analysis of genetic diversity of nine native populations of wild sugarcane, saccharum spontaneum, from sichuan, china.saccharum spontaneum is a wild sugarcane species that is native to and widely distributed in china. it has been extensively used in sugarcane breeding programs, and is being tested for the development of bioenergy cultivars. in order to provide basic information for the exploitation of this species, we analyzed genetic variation among and within native s. spontaneum populations collected from sichuan, china. eighty plants from nine native populations were sampled. twenty-one sequence-related amp ...201222614352
vermicomposting and anaerobic digestion - viable alternative options for terrestrial weed management - a review.the management of terrestrial weed is of great concern for the scientific community as these weeds cause adverse effect in different ecosystems like forest, agriculture and urban. the widespread of these weeds by their adaptive capability and morphological advancement is difficult to control. parthenium hysterophorous, lantana camara, saccharum spontaneum, ageratum conyzoides are the weeds that spread all over the world. there are various management practices employed for the control of this wee ...201829349043
genetic mapping in sugarcane, a high polyploid, using bi-parental progeny: identification of a gene controlling stalk colour and a new rust resistance gene.modern sugarcane cultivars (saccharum spp) are highly polyploïd and aneuploid interspecific hybrids (2n = 100-130). two genetic maps were constructed using a population of 198 progeny from a cross between r570, a modern cultivar, and mq76-53, an old australian clone derived from a cross between trojan (a modern cultivar) and ses528 (a wild saccharum spontaneum clone). a total of 1,666 polymorphic markers were produced using 37 aflp primer combinations, 46 ssrs and 9 rflp probes. linkage analysis ...200616552554
molecular dissection of complex traits in autopolyploids: mapping qtls affecting sugar yield and related traits in sugarcane.mapping quantitative trait loci (qtls) for sugar yield and related traits will provide essential information for sugarcane improvement through marker-assisted selection. two sugarcane segregating populations derived from interspecific crosses between saccharum offinarum and saccharum spontaneum with 264 and 239 individuals, respectively, were evaluated in three replications each for field performance from 1994 to 1996 at weslaco, texas. these two populations were analyzed for a total of 735 dna ...200212582536
statistical aspects of genetic mapping in autopolyploids.many plant species of agriculture importance are polyploid, having more than two copies of each chromosome per cell. in this paper, we describe statistical methods for genetic map construction in autopolyploid species with particular reference to the use of molecular markers. the first step is to determine the dosage of each dna fragment (electrophoretic band) from its segregation ratio. fragments present in a single dose can be used to construct framework maps for individual chromosomes. fragme ...199910415330
quantitative chromosome map of the polyploid saccharum spontaneum by multicolor fluorescence in situ hybridization and imaging methods.somatic chromosomes of a wild relative of sugarcane (saccharum spontaneum l.) anther culture-derived clone (ap 85-361, 2n = 32) were identified and characterized by computer-aided imaging technology and molecular cytological methods. the presence of four satellite chromosomes and four nearly identical chromosome sets suggests that the clone is a tetrahaploid with the basic number x = 8. a quantitative chromosome map, or idiogram, was developed using image analysis of the condensation pattern (cp ...199910380803
ribosomal dna variations in erianthus, a wild sugarcane relative (andropogoneae-saccharinae).variation at the 18s+26s and 5s ribosomal dna loci was assessed on 62 erianthus michx. clones, representing 11 species, and 15 clones from two saccharum l. species used as a reference. genus-specific markers for erianthus michx. sect. ripidium henrard (old world species) were identified. ribosomal dna units in erianthus sect. ripidium exhibited an additional bamhi site compared to saccharum, and 5s units showed length and restriction-site differences between erianthus and saccharum. these marker ...199624166398
quantitative analysis of the effect of selection history on sugar yield adaptation of sugarcane clones.an objective of the csr sugarcane breeding programme in australia was to assess the scope for broadening the genetic base of the commercial sugarcane germ plasm through interspecific hybridization with saccharum spontaneum clones. the contribution of both selection history and s. spontaneum to sugar yield and its components was investigated in the germ plasm pool assembled. the analysis was conducted on a data-set of 256 clones, consisting of parents and full-sib families generated from 32 bipar ...199424190405
rflp linkage map and genome analysis of saccharum spontaneum.an rflp linkage map of the wild sugarcane species saccharum spontaneum l. (2n = 8x = 40-128) was constructed, comprising 216 loci, detected by 116 dna probes, and distributed over 44 linkage groups. at a density of at least one marker every 25-cm interval, the coverage of the genome was estimated as 86%. for the generation of rflp markers, probes were surveyed from seven dna libraries: three sugarcane cdna, one oat cdna, one rice cdna, and one barley cdna, as well as one sugarcane genomic. sixty ...199318470024
a genetic linkage map of saccharum spontaneum l. 'ses 208'.the arbitrarily primed polymerase chain reaction was used to detect single-dose polymorphisms that, in turn, were used to generate a linkage map of a polyploid relative of cultivated sugarcane, saccharum spontaneum 'ses 208' (2n = 64). the mapping population was composed of 88 progeny from a cross between ses 208 and a diploidized haploid derived from ses 208 by anther culture, adp 85-0068. this cross allowed direct analysis of meiosis in ses 208 and gametic segregation ratios to be observed. on ...19938375659
high output genetic mapping of polyploids using pcr-generated markers.the polymerase chain reaction (pcr) with arbitrarily selected primers has been established as an efficient method to generate fingerprints that are useful in genetic mapping and genomic fingerprinting. to further increase the productivity of mapping and fingerprinting efforts, we have altered existing protocols to include the use of the stoffel fragment, which is derived from genetically engineered taq polymerase. we also optimized the thermal profile of the reaction to increase the number of us ...199324193389
biochemical genetic markers in sugarcane.isozyme variation was used to identify biochemical markers of potential utility in sugarcane genetics and breeding. electrophoretic polymorphism was surveyed for nine enzymes among 39 wild and noble sugarcane clones, belonging to the species most closely related to modern varieties. up to 114 distinct bands showing presence versus absence type of variation were revealed and used for qualitative characterization of the materials. multivariate analysis of the data isolated the erianthus clone samp ...198924225682
production of haploid saccharum spontaneum l. - comparison of media for cold incubation of panicle branches and for float culture of anthers.means were sought to increase the frequency of haploid production from anther cultures of saccharum spontaneum l. excised preemerged panicle branches were incubated in modified murashige-skoog (ms), nitsch (h), gamborg (b5), and chu (n6) media at 10 °c for 4 to 10 weeks prior to removal of the anthers for culturing at 27 °c. anthers, either continuously cultured on liquid media or floated on liquid media 1 to 8 weeks prior to nurse culture, produced visible calli in 30 days. nearly 1% of the ant ...198423195613
developmental and biochemical characteristics of cold-treated anthers of saccharum spontaneum.the role of cold in stimulating androgenic haploid development was evaluated by assessing morphological and biochemical changes occurring in saccharum spontaneum anthers during 3 weeks of incubation of panicle sections at 10 °c. during incubation, anthers increased in size; and although many microspores lost viability, those that survived proceeded to divide symmetrically rather than asymmetrically as found in normal microsporogenesis. anthers which contained dividing microspores differed bioche ...198423194722
quantitative genetics of sugarcane : iii. potential for sucrose selection in saccharum spontaneum.1. a project has been initiated to explore the possibilities of selection for higher levels of sucrose storage in the wild species s. spontaneum, using a representative sample of parental clones under conditions of natural crossing. 2. the aim of the programme is the development of superior clones to represent the wild species in conventional breeding programmes, which involve repeated backcrossing to s. officinarum. 3. an analysis of the first generation following the intercrossing of 21 parent ...196924435287
the effect of long periods of darkness on flowering in saccharum spontaneum soraparai, 270. 194720243386
identification and cloning of molecular markers for uv-b tolerant gene in wild sugarcane (saccharum spontaneum l.).previously we have selected wild sugarcane (saccharum spontaneum l.) sterile lines that are tolerant or susceptible to uv-b radiation based on response index (ri) in a field screening test. the ri was established according to plant height, tiller number, leaf index, total biomass and brix under enhanced ultraviolet-b (uv-b, 280-310 nm) radiation. in this experiment, molecular markers linked to the uv-b tolerant and susceptible genes were identified and cloned. rapd (randomly amplified polymorphi ...201121925894
kushneria pakistanensis sp. nov., a novel moderately halophilic bacterium isolated from rhizosphere of a plant (saccharum spontaneum) growing in salt mines of the karak area in pakistan.the taxonomic position of a gram-stain negative, moderately halophilic bacterium, designated nccp-934(t), was investigated using polyphasic taxonomic approach. the strain nccp-934(t) was isolated from rhizosphere of a plant (saccharum spontaneum, family poaceae) growing in salt mines area in the karak district of khyber pakhtunkhwa province, pakistan. cells of strain nccp-934(t) are rod shaped and motile. the bacterium is strictly aerobic, can grow at a temperature range of 10-40 °c (optimum at ...201525631404
isolation and characterization of a satellite dna family in the saccharum complex.eacir1, a 371-bp erianthus-specific satellite dna sequence, was cloned from taqi restricted genomic dna after agarose-gel electrophoresis. this sequence has 77% homology with a 365-bp satellite of helictotrichon convolutum and 72% homology with a 353-bp tandem repeat sequence from oryza sativa. pcr primers defined in the conserved regions of these repetitive sequences were used to isolate other satellite dnas in different representatives of the saccharum complex: socir1 in saccharum officinarum, ...19989924794
feeding preferences of the asian elephant (elephas maximus) in nepal.nepal provides habitat for approximately 100-125 wild asian elephants (elephas maximus). although a small proportion of the world population of this species, this group is important for maintaining the genetic diversity of elephants and conservation of biodiversity in this region. knowledge of foraging patterns of these animals, which is important for understanding their habitat requirements and for assessing their habitat condition, is lacking for the main areas populated by elephants in nepal. ...201627855704
ecotechnological approach for consolidation of uranium tailings.present study has been undertaken to consolidate radioactivity in uranium mill tailings at jaduguda, jharkhand, india.tailings that remain after processing of ore are released in tailing ponds specially designed for the purpose. the degraded tailing ponds have been capped with 30 cm. thick soil cover. for cosolidation of radioactivity in the tailings firstly the selected plant species should not have any socioeconomic relevance in that area and secondly, uptake of uranium by selected plants has ...201123029938
development of corn starch based green composites reinforced with saccharum spontaneum l fiber and graft copolymers--evaluation of thermal, physico-chemical and mechanical properties.in this paper, corn starch based green composites reinforced with graft copolymers of saccharum spontaneum l. (ss) fiber and methyl methacrylates (mma) and its mixture with acrylamide (aam), acrylonitrile (an), acrylic acid (aa) were prepared. resorcinol-formaldehyde (rf) was used as the cross-linking agent in corn starch matrix and different physico-chemical, thermal and mechanical properties were evaluated. the matrix and composites were found to be thermally more stable than the natural corn ...201020395134
phytoremediation potentials of selected tropical plants for ethidium bromide.research and development has its own benefits and inconveniences. one of the inconveniences is the generation of enormous quantity of diverse toxic and hazardous wastes and its eventual contamination to soil and groundwater resources. ethidium bromide (etbr) is one of the commonly used substances in molecular biology experiments. it is highly mutagenic and moderately toxic substance used in dna-staining during electrophoresis. interest in phytoremediation as a method to solve chemical contaminat ...200718062483
biotrophic interaction of sporisorium scitamineum on a new host--saccharum spontaneum.sporisorium scitamineum is a biotrophic smut fungus harbored inside the smut gall on the top internodal region of saccharum spontaneum, a wild relative of sugarcane (saccharum officinarum). the interactions of spined conidia of s. scitamineum with s. spontaneum were examined during the different stages of plant growth starting from the bud stage to the decaying stage. the spores in the soil from the polyetic inocula grew into confined epidermal cells of the buds and finally sporulated in the top ...201626642345
alkaline pretreatment methods followed by acid hydrolysis of saccharum spontaneum for bioethanol production.different alkaline pretreatment methods (naoh, naoh+10% urea and aqueous ammonia) were optimized for maximum delignification of saccharum spontaneum at 30°c. maximum delignification were obtained as 47.8%, 51% and 48% from naoh (7% naoh, 48h, and 10% biomass loading), naoh+urea (7% naoh+10% urea, 48 h and 10% biomass loading) and 30% ammonia (40 days and 10% biomass loading) respectively. h(2)so(4) 60% (v/v), 10% biomass loading at 30°c for 4h, were optimized conditions to solubilize the cellulo ...201222985852
co-expression network analysis reveals transcription factors associated to cell wall biosynthesis in sugarcane.sugarcane is a hybrid of saccharum officinarum and saccharum spontaneum, with minor contributions from other species in saccharum and other genera. understanding the molecular basis of cell wall metabolism in sugarcane may allow for rational changes in fiber quality and content when designing new energy crops. this work describes a comparative expression profiling of sugarcane ancestral genotypes: s. officinarum, s. spontaneum and s. robustum and a commercial hybrid: rb867515, linking gene expre ...201626820137
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