| nucleotide sequence analysis of virus isolates indicates the presence of three potyvirus species in allium plants. | cdnas of potyviruses from allium plants cultivated in different parts of the world were cloned by rt-pcr with a common primer for amplifying the 3' terminal genomic rnas of onion yellow dwarf virus (oydv), leek yellow stripe virus (lysv) and, probably, of closely related potyviruses. their nucleotide sequences bearing the viral coat protein (cp) gene and the 3' non-coding sequence were determined and compared. the degree of their sequence similarities clearly differentiated the respective viruse ... | 1998 | 9505969 |
| characterisation of some carla- and potyviruses from bulb crops in china. brief report. | conserved carla- and potyvirus primers were used in rt-pcr to amplify virus fragments from garlic and other bulb crops in china and the fragments were subsequently sequenced and compared in phylogenetic analyses. garlic plants from henan, hubei, jiangsu, shangdong and yunnan provinces all contained at least one isolate each of garlic latent virus (genus carlavirus), onion yellow dwarf virus (oydv, genus potyvirus) and leek yellow stripe virus (lysv, genus potyvirus). the complete sequence of a z ... | 2002 | 11890533 |
| characterisation of the welsh onion isolate of shallot yellow stripe virus from china. | the host range and nucleotide sequence of shallot yellow stripe virus (sysv) from welsh onion in shandong province, china is described. of the plants tested, only shallot and welsh onion became infected but most shallot plants were symptomless. the complete sequence of one isolate (10429 nt) and the 3'-terminal 3540 nts of a second isolate were determined. they had c. 90% nt identity to one another and to published (partial) sequences of sysv. sysv was most closely related to onion yellow dwarf ... | 2005 | 15968472 |
| identification and sequence analysis of potyviruses infecting crops in vietnam. | fifty-two virus isolates from 13 distinct potyvirus species infecting crops in vietnam were identified and the 3' region of each genome was sequenced. the viruses were: bean common mosaic virus (bcmv), potato virus y (pvy), sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv), chilli veinal mottle virus (chivmv), zucchini yellow mosaic virus (zymv), leek yellow stripe virus (lymv), shallot yellow stripe virus (sysv), onion yellow dwarf virus (oydv), turnip mosaic virus (tumv), dasheen mosa ... | 2008 | 17906829 |
| further evidence that shallot yellow stripe virus (sysv) is a distinct potyvirus and reidentification of welsh onion yellow stripe virus as a sysv strain. | abstract an antiserum to shallot yellow stripe virus (sysv) was raised and used in combination with a range of other antisera to potyviruses of allium spp. in electron microscopic decoration experiments. the serological results corroborated an earlier finding that the type isolates of sysv and welsh onion yellow stripe virus (woysv) are closely related to each other and only distantly related to onion yellow dwarf (oydv) and leek yellow stripe (lysv) viruses, the two other major potyviruses infe ... | 1999 | 18944789 |
| protein-protein interactions in two potyviruses using the yeast two-hybrid system. | interactions between all ten mature proteins of the potyviruses soybean mosaic virus (pinellia ternata isolate) and shallot yellow stripe virus were investigated using yeast two-hybrid (y2h) assays. consistently strong self-interactions were found between the pairs of hc-pro, vpg, nia-pro, nib and cp in both viruses. apart from the nib, such interactions have been previously reported for some other potyviruses. the 6k1/nia-pro combination gave a consistently moderate to strong interaction in bot ... | 2009 | 19189854 |
| interaction between potyvirus p3 and ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) of host plants. | the p3 protein encoded by shallot yellow stripe virus onion isolate (sysv-o) interacted in the yeast two-hybrid (y2h) system and in co-immunoprecipitation (co-ip) assays with the large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) protein that is encoded by the rbcl gene of its onion host. dissection analysis by y2h showed that the main part of sysv p3 (amino acids 1-390) and onion rbcl (amino acids 1-137) were responsible for the interaction. the p3 proteins encoded b ... | 2011 | 21400205 |
| the first complete genome sequence of iris severe mosaic virus. | the first complete genome sequence of ismv was determined by deep sequencing of a small rna library constructed from ismv-infected samples and rapid amplification of cdna ends (race) pcr. the ismv genome consists of 10,403 nucleotides excluding the poly(a) tail and contains a large open reading frame encoding a polyprotein of 3316 amino acids. putative proteolytic cleavage sites were identified by blast analysis. the ismv polyprotein showed highest amino acid sequence identity to that encoded by ... | 2016 | 26729478 |