isolation and properties of glyceraldehyde-3-phosphate dehydrogenase from a sturgeon from the caspian sea and its interaction with spin-labeled nad+ derivatives. | glyceraldehyde-3-phosphate dehydrogenase (d-glyceraldehyde-3-phosphate: nad+ oxidoreductase (phosphorylating), ec 1.2.1.12) was isolated from a sturgeon, huso huso, from the caspian sea. it is closely related to the enzyme from a pacific sturgeon, acipenser transmontanus, with respect to amino acid composition, steady-state kinetics and coenzyme binding. the latter, as studied by means of a spin-labeled derivative of nad+, is negatively cooperative exhibiting a hill coefficient of 0.84 at 12 deg ... | 1981 | 6266490 |
developmental structure of the vertebral column, fins, scutes and scales in bester sturgeon, a hybrid of beluga huso huso and sterlet acipenser ruthenus. | in the larval bester, a hybrid sturgeon of beluga huso huso and sterlet acipenser ruthenus, development of cartilage around the notochord began 7 days post hatch (dph) (14.0 mm, total length, l(t)). the vertebral cartilage develops in the following sequence: basidorsals and basiventrals, neural canals, neural spines and ribs. the development of ribs remained incomplete in the largest specimen (181 dph, 179 mm l(t) ) that was examined. endoskeletal development of the fins began 4 dph for the dors ... | 2012 | 23130694 |
biochemical characterisation and assessment of fibril-forming ability of collagens extracted from bester sturgeon huso huso × acipenser ruthenus. | collagens purified from bester sturgeon organs were characterised biochemically, and their fibril-forming abilities and fibril morphologies formed in vitro clarified. yields of collagens were 2.1%, 11.9%, 0.4%, 18.1%, 0.4%, 0.8% and 0.03% (collagen dry weight/tissue wet weight) from scales, skin, muscle, swim bladder, digestive tract, notochord and snout cartilage, respectively. using sds-page and amino acid composition analyses, collagens from scales, skin, muscle, the swim bladder and digestiv ... | 2014 | 24799243 |
activation of the albino sterlet acipenser ruthenus eggs by uv-irradiated bester hybrid spermatozoa to provide gynogenetic progeny. | meiotic gynogenesis was induced in the albino form of sterlet acipenser ruthenus by activation of eggs with uv-irradiated bester (huso huso x acipenser ruthenus) spermatozoa followed by inhibition of the second meiotic division performed by a heat shock. obtained putative gynogenetic progeny were all albinos. the genetic verification based on three microsatellite dna markers confirmed the only maternal inheritance of the progeny from the gynogenetic experimental groups. cytogenetic analysis prov ... | 2015 | 25858073 |
novel technique for visualizing primordial germ cells in sturgeons (acipenser ruthenus, a. gueldenstaedtii, a. baerii, and huso huso). | primordial germ cells (pgcs) are the origin of all germ cells in developing embryos. in the sturgeon embryo, pgcs develop from the vegetal hemisphere, which mainly acts as an extraembryonic source of nutrition. current methods for studying sturgeon pgcs require either killing the fish or using costly and time-consuming histological procedures. here, we demonstrate that visualization of sterlet (acipenser ruthenus>) pgcs in vivo is feasible by simply labeling the vegetal hemisphere with fluoresce ... | 2015 | 26134864 |
bioaccumulation of cd, pb and zn in the edible and inedible tissues of three sturgeon species in the iranian coastline of the caspian sea. | the accumulations of cd, pb and zn were determined in edible and inedible tissues of persian sturgeon (acipenser persicus; n=27), stellate sturgeon (acipenser stellatus; n=5) and beluga (huso huso; n=4) collected from coastal waters of the south caspian sea from march to april 2011. concentrations of metals evaluated in the caviar, muscle, liver, kidney, gills, ovary and heart of the three species of sturgeons have been assessed using the flame and graphite furnace atomic absorption spectrophoto ... | 2013 | 22990022 |
developmental transcription of genes putatively associated with growth in two sturgeon species of different growth rate. | in the present study, we surveyed developmental changes in the transcription of growth hormone (gh), insulin-like growth factor-i (igf-i), ghrelin (ghrl) and vascular endothelial growth factor (vegf) genes in the largest freshwater fish, european sturgeon (beluga, huso huso) and compared the same parameters to that of its phylogenically close moderate-sized species, persian sturgeon (acipenser persicus). the transcripts of gh, igf-i, ghrl and vegf were detected at all developmental time-points o ... | 2013 | 23229003 |
[a research on ig heavy chain constant region of five acipenseridae]. | analyzed the immunoglobulin (ig) heavy chain constant regions of russian sturgeon (a. gueldenstaedtii); sterlte sturgeon (a. ruthenus); amur sturgeon (a. schrenckii); chinese sturgeon (a. sinensis) and great sturgeon (huso huso) with molecular biology and bioinformatics methods. we cloned igh nucleic acid sequences by rt-pcr using the specific primer, then determined the characteristics and functions of the amino acid sequences and plotted out the sequence into four sub-regions (ch1-ch4), of whi ... | 2006 | 17035183 |
immunoglobulin heavy chain constant region of five acipenseridae: cdna sequence and evolutionary relationship. | the immunoglobulin m (igm) heavy chain constant region genes of russian sturgeon (acipenser gueldenstaedtii), sterlte sturgeon (acipenser ruthenus), amur sturgeon (acipenser schrenckii), chinese sturgeon (acipenser sinensis) and great sturgeon (huso huso) were cloned and analyzed with molecular biology and bioinformatics methods. we cloned igh nucleic acid sequences by rt-pcr using the specific primer, then determined the characteristics and functions of the amino acid sequences. the gene contai ... | 2007 | 17234432 |
[polymorphism of the mitochondrial dna control region in eight sturgeon species and development of a system for dna-based species identification]. | intraspecific and interspecific nucleotide sequence variations of the mtdna control region (d-loop) were studied with mtdnas isolated from tissue specimens of more than 1400 sturgeons of nine species: russian sturgeon acipenser gueldenstaedtii, persian sturgeon a. persicus, siberian sturgeon a. baerii, amur sturgeon a. schrenkii, fringebarbel sturgeon a. nudiventris, sterlet a. ruthenus, stellate sturgeon a. stellatus, beluga huso huso, and kaluga h. dauricus. the results were used to analyze th ... | 2008 | 18767539 |
comparative protein profiles: potential molecular markers from spermatozoa of acipenseriformes (chondrostei, pisces). | sturgeon and paddlefish (acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. in this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: siberian sturgeon (acipenser baerii), sterlet (a. ruthenus), russian sturgeon (a. gueldenstaedtii), starry sturgeon (a. stellatus), beluga (huso huso), and mississippi paddlefish (polyodon spathula). protein profiles ... | 2010 | 20869341 |
effects of dietary spirulina platensis on growth performance, humoral and mucosal immune responses and disease resistance in juvenile great sturgeon (huso huso linnaeus, 1754). | dietary supplementation of spirulina platensis at different levels (0% control, 2.5%, 5% and 10%) was evaluated to find out the effects on growth performance, digestive enzyme activities, humoral and skin innate immune responses and disease resistance in the great sturgeon (huso huso). after 8 weeks of experimental trial, growth parameters, intestinal lactic acid bacteria count, protease and lipase activities were significantly high in 10% s. platensis fed group (p < 0.05). similarly, in this gr ... | 2016 | 27506276 |
Acquisition of glial cells missing 2 enhancers contributes to a diversity of ionocytes in zebrafish. | Glial cells missing 2 (gcm2) encoding a GCM-motif transcription factor is expressed in the parathyroid in amniotes. In contrast, gcm2 is expressed in pharyngeal pouches (a homologous site of the parathyroid), gills, and H(+)-ATPase-rich cells (HRCs), a subset of ionocytes on the skin surface of the teleost fish zebrafish. Ionocytes are specialized cells that are involved in osmotic homeostasis in aquatic vertebrates. Here, we showed that gcm2 is essential for the development of HRCs and Na(+)-Cl ... | 2011 | 21858216 |
Lactococcus lactis subsp. lactis infection in Bester sturgeon, a cultured hybrid of Huso huso×Acipenser ruthenus, in Taiwan. | Approximately 5300 hybrid sturgeons with an average body weight of 600-800g were farmed in 3 round tankers measuring 3m in diameter each containing 28,000L of aerated groundwater. According to the owner's description, the diseased fish had anorexia, pale body color, and reddish spots on the abdomen. The morbidity and lethality rates in this outbreak were about 70% (3706/5300) and 100% (3706/3706), respectively. The clinical examination revealed enteritis, enlarged abdomen, and rapid respiration ... | 2011 | 22098776 |
first detection of a viral agent causing disease in farmed sturgeon in russia. | a virus was isolated from siberian sturgeon acipenser baeri and bester (beluga huso huso x sterlet a. ruthenus hybrid) fingerlings in sso-2, ssf-2 and wssk-1 cell lines during an acute epizootic on a large fish farm producing fertilised sturgeon eggs and fry. transmission electron microscopic examination of samples from both inoculated cell cultures and skin of affected fish revealed viral particles with a herpesvirus-like morphology. the etiological role of the siberian sturgeon herpesvirus (sb ... | 2009 | 20066954 |
molecular cloning of cdna of mammalian and chicken ii gonadotropin-releasing hormones (mgnrhs and cgnrh-ii) in the beluga (huso huso) and the disruptive effect of methylmercury on gene expression. | two gonadotropin-releasing hormone (gnrh) isoforms were identified in the beluga (huso huso) brain by cdna sequencing: prepro-mammalian gnrh (mgnrh) and prepro-chicken gnrh-ii (cgnrh-ii). the nucleotide sequences of the beluga mgnrh and cgnrh-ii precursors are 273 and 258 base pairs (bp) long, encoding peptides of 91 and 86 amino acids, respectively. to investigate the effect of methylmercury (mehg) on gnrh gene expression, animals were fed with four diets containing increasing levels of mehg (0 ... | 2010 | 19821139 |
contamination by organochlorine compounds in the edible tissue of four sturgeon species from the caspian sea (iran). | this study focused on accumulation of organochlorine compounds (ocs), including dichloro-diphenyl-trichloroethanes (ddts), hexachlorobenzene (hcb), hexachlorocyclohexanes (hchs), and polychlorinated biphenyls (pcbs) accumulation in the muscle of four sturgeon (persian sturgeon, acipenser persicus; stellate sturgeon, acipenser stellatus; ship sturgeon, acipenser nudiventris and beluga sturgeon, huso huso) from the southern caspian sea. the ddt group was prominent in all of the sturgeon muscle tes ... | 2008 | 18657287 |
concentrations and risks of organic and metal contaminants in eurasian caviar. | caviar (fish roe of sturgeon) may contain high levels of contaminants. concentrations of organic contaminants, including ddt, hexachlorocyclohexanes (hchs), polychlorinated biphenyls (pcbs), brominated flame retardants (polybrominated diphenyl ethers (pbdes)), and 23 metals were measured in three species of caviar (acipenser huso huso, acipenser gueldenstaedti, and acipenser stellatus) imported from azerbaijan, bulgaria, iran, and russia just prior to the 2006 export ban. pcb concentrations aver ... | 2008 | 17681601 |
trace elements accumulation in edible tissues of five sturgeon species from the caspian sea. | this study is focused on twenty trace elements (ag, ba, bi, cd, co, cr, cs, cu, ga, in, mn, mo, pb, rb, sb, sn, sr, ti, v, zn) accumulation in muscles of five sturgeon species (acipenser guldenstaedti, a. persicus, a. nudiventris, a. stellatus and huso huso) from the southern part of the caspian sea. moreover the relationships between some biological characteristics and the levels of the selected elements as well as inter-elemental relationships were assessed. the samples (10 specimens for each ... | 2005 | 15727302 |
concentrations of trace elements in muscle of sturgeons in the caspian sea. | concentrations of 21 trace elements were determined in muscle of beluga (huso huso), persian sturgeon (acipenser persicus), russian sturgeon (acipenser gueldenstaedtii), ship sturgeon (acipenser nudiventris) and stellate sturgeon (acipenser stellatus) collected from coastal regions of azerbaijan, iran, kazakhstan and turkmenistan, surrounding the caspian sea during 2000-2001. concentrations of mn, co, cu, mo, sn, hg, pb and bi in the muscle were apparently different among the five species of stu ... | 2004 | 15530523 |
chromosomal mapping of 18s-28s and 5s rrna genes by two-colour fluorescent in situ hybridization in six sturgeon species. | the number and distribution of the 18s-28s and 5s rrna (rdna) gene sequences were examined on mitotic chromosomes of six sturgeon species by two-colour in situ hybridization. four of the six species, huso huso, acipenser stellatus, acipenser sturio, and acipenser ruthenus, with about 120 chromosomes, showed from six to eight 18s-28s rdna signals, while 5s rdna signals were on only one chromosome pair. the two species with 250-270 chromosomes, acipenser baerii and acipenser transmontanus, showed ... | 2003 | 12834065 |
dna content in eurasian sturgeon species determined by flow cytometry. | the nuclear dna content in 10 species of chondrostean fishes was measured by flow cytometry. the sterlet acipenser ruthenus blood cells were used as an internal standard. the sterlet dna content was calculated on the basis of comparison with the xenopus laevis blood cells, 2c = 6.30 pg. in the tetraploid a. ruthenus and a. stellatus the dna content comprises 3.74 pg/nucleus and is practically invariant; in huso dauricus it is almost the same, 3.74-3.81 pg; and in a. nudiventris it is a little hi ... | 1993 | 8513694 |
[the characteristics of the circadian motor activity of sturgeons and their hybrids studied by a motion picture-tv method]. | technique of slow-motion shot was used for simultaneous automatic recording of behaviour of sturgeon species acipenser nudiventris and its hybrid with white sturgeon, huso huso. the hybrids and the individuals of the paternal species were placed in two separate aquaria under the conditions of constant light. cinematographic recording was performed each hour throughout 3 days during 30 s per hour. significant differences between two kinds of sturgeons were found in the locomotor activity, traject ... | 1994 | 8023569 |
effects of dietary onion (allium cepa) powder on growth, innate immune response and hemato-biochemical parameters of beluga (huso huso linnaeus, 1754) juvenile. | the present study was aimed at determining the effects of dietary onion powder on growth, innate immune response and hemato-biochemical parameters of beluga juvenile (huso huso). basal diets containing onion powder 0 (control), 0.5 and 1% of feed were fed to beluga juvenile. at the end of the experiment, the highest weight gain (wg%) and specific growth rate (sgr) was observed in group fed with 1% onion (p < 0.05). there were no significant difference (p > 0.05) about feed conversion ratio (fcr) ... | 2015 | 26067169 |
effect of ginger- and garlic-supplemented diet on growth performance, some hematological parameters and immune responses in juvenile huso huso. | physiological parameters and growth performance of huso huso fingerlings fed with diet supplemented with herbal plants were studied in this study. dietary treatments were 0 g (= control), 1.0 g (garlic) and 1.0 g (ginger) of 100 g(-1) feed for 60 days. specific growth rate, body weight gain and condition factor were significantly increased in ginger treatment at the end of the trial. highest levels of erythrocyte and hematocrit were observed in fingerlings ginger group after 60 days. globulin in ... | 2014 | 24122196 |
effects of dietary administration of rose hip and safflower on growth performance, haematological, biochemical parameters and innate immune response of beluga, huso huso (linnaeus, 1758). | the aim of this study was to investigate effects of two dietary medicinal herbs, rose hip (rosa canina) and safflower (carthamus tinctorius) supplementation on growth performance, haematological, biochemical parameters and innate immune response of in juvenile beluga, huso huso. fish (26.3 ± 0.4 g) were allocated into 15 tanks (20 fish per tank) and triplicate groups were fed a control diet or diets containing 1% and 2% of medicinal herbs, respectively. feed conversion ratio (fcr), specific grow ... | 2016 | 27773731 |
a visual pigment of the sturgeon retina. | the visual pigments of hybrid sturgeon (a cross between acipenser ruthenus (male) and huso huso (female) have been studied both by the methods of incomplete partial bleaching and hplc analysis. on the basis of the results obtained the relationship between the structure of opsins and the spectral characteristics of visual pigments is discussed. | 1989 | 2920798 |
lymphoid organs in sturgeons (acipenseridae). | lymphoid (lymphomyeloid) tissues in sturgeons (hybrid sturgeon, huso huso x acipenser ruthenus, and white pacific sturgeon, a. transmontanus) were investigated by dissection, histology and transmission electron microscopy. the main lymphomyeloid tissues are the thymus, the spleen, the anterior part of the kidney, the meningeal myeloid tissue, the pericardial tissue and lymphoid masses of the intestine, especially in the spiral valve. the kidney is the main hemopoietic tissue. the meningeal tissu ... | 1986 | 3765338 |
[localization of cells connected with different tropic functions in the hypophysis of the young of huso huso l. x acipenser ruthenus l., chondrostei]. | a number of histological and histochemical methods were used in order to detect different secretory cells in the hypophysis of young huso huso l. x acipenser ruthenus l., chondrostei. special attention was given to somatotropic cells. for their detection the following staining methods were used: orange g - acidic red 2c - acidic green g after fixation in the mixture of hgcl2 with formalin. in the dorsal part of the hypophysis having the appearance of vertical folds pressed into the hypophysis fr ... | 1976 | 971119 |
on the adrenergic system of ganoid fish: the beluga, huso huso (chondrostei). | the adrenergic system of the beluga, huso huso, was studied by glyoxylic acid fluorescence histochemistry, analyses of catecholamine content in various organs and studies of the effects of acetylcholine and adrenaline on isolated strip preparations from blood vessels, spleen, atrium and ventricle. chromaffin cells were found mainly in the walls of the posterior cardinal veins, and to some extent also in the wall of the celiaco-mesenteric artery. the plasma concentration of adrenaline was high en ... | 1981 | 7304205 |
physiological characteristics and stress resistance of great sturgeon (huso huso) juveniles fed with vitamins c, e, and hufa-enriched artemia urmiana nauplii. | this study was carried out to examine the effect of artemia urmiana nauplii enriched with hufa, and vitamins c and e on stress tolerance, hematocrit, and biochemical parameters of great sturgeon, huso huso juveniles. cod liver oil (epa 18% and dha 12%), ascorbyl-6-palmitate and alpha-tocopherol acetate were used as lipid, and vitamin c and e sources, respectively. beluga juveniles at the stage of first feeding (69.7 +/- 5.9 mg body weight) were randomly divided into five treatments and three tan ... | 2010 | 19459059 |