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[enterobacter asburiae isolated from clinical specimens]. 19883150411
chromosomal ampc genes in enterobacter species other than enterobacter cloacae, and ancestral association of the act-1 plasmid-encoded cephalosporinase to enterobacter asburiae.the amplification and sequence of ampc genes in enterobacter asburiae, enterobacter cancerogenus, enterobacter dissolvens, enterobacter hormaechei and enterobacter intermedius bring the number of known cephalosporinase sequences from the genus enterobacter to seven. expression in escherichia coli of the ampc genes from e. asburiae, e. hormaechei and e. intermedius established the functional nature of these genes. ampc from e. asburiae shows 96.5% identity to bla(act-1) encoding a plasmid-borne c ...200212023082
colonization of arabidopsis thaliana with salmonella enterica and enterohemorrhagic escherichia coli o157:h7 and competition by enterobacter asburiae.enteric pathogens, such as salmonella enterica and escherichia coli o157:h7, have been shown to contaminate fresh produce. under appropriate conditions, these bacteria will grow on and invade the plant tissue. we have developed arabidopsis thaliana (thale cress) as a model system with the intention of studying plant responses to human pathogens. under sterile conditions and at 100% humidity, s. enterica serovar newport and e. coli o157:h7 grew to 10(9) cfu g(-1) on a. thaliana roots and to 2 x 1 ...200312902287
isolation of endophytic diazotroph pantoea agglomerans and nondiazotroph enterobacter asburiae from sweetpotato stem in japan.to isolate and identify diazotrophic endophytes in the stem of japanese sweetpotato cv. koganesengan.200414687210
incidence of antibiotic-resistant klebsiella pneumoniae and enterobacter species in freshwater wetlands.the aim of this study was to assess the incidence of enterobacteriaceae (potential human and animal pathogens) in wetlands.200415482436
reassignment of enterobacter dissolvens to enterobacter cloacae as e. cloacae subspecies dissolvens comb. nov. and emended description of enterobacter asburiae and enterobacter kobei.the taxonomic position of enterobacter dissolvens was re-evaluated based on the analysis of the type strain atcc 23373t and three clinical isolates. the strains were assigned to the genetic cluster of the species by phylogenetic sequence analysis in the frame of a recent population genetic study. the relatedness of e. dissolves to the other species of the e. cloacae complex was analyzed by dna-dna hybridization studies based on melting profiles in microplates. the genetic cluster of e. dissolven ...200515900966
complete fermentation of xylose and methylglucuronoxylose derived from methylglucuronoxylan by enterobacter asburiae strain jdr-1.acid pretreatment is commonly used to release pentoses from the hemicellulose fraction of cellulosic biomass for bioconversion. the predominant pentose in the hemicellulose fraction of hardwoods and crop residues is xylose in the polysaccharide methylglucuronoxylan, in which as many as one in six of the beta-1,4-linked xylopyranose residues is substituted with alpha-1,2-linked 4-o-methylglucuronopyranose. resistance of the alpha-1,2-methylglucuronosyl linkages to acid hydrolysis results in relea ...200919011070
isolation and characterization of rhamnolipid-producing bacterial strains from a biodiesel facility.novel strains of rhamnolipid-producing bacteria were isolated from soils at a biodiesel facility on the basis of their ability to grow on glycerol as a sole carbon source. strains were identified as acinetobacter calcoaceticus, enterobacter asburiae, enterobacter hormaechei, pantoea stewartii, and pseudomonas aeruginosa. the strains of the former five species were found to produce rhamnolipids in quantities the same as, or similar to, coisolated strains of p. aeruginosa. measurements of surface ...200919473254
genetic engineering of enterobacter asburiae strain jdr-1 for efficient production of ethanol from hemicellulose hydrolysates.dilute acid pretreatment is an established method for hydrolyzing the methylglucuronoxylans of hemicellulose to release fermentable xylose. in addition to xylose, this process releases the aldouronate methylglucuronoxylose, which cannot be metabolized by current ethanologenic biocatalysts. enterobacter asburiae jdr-1, isolated from colonized wood, was found to efficiently ferment both methylglucuronoxylose and xylose in acid hydrolysates of sweet gum xylan, producing predominantly ethanol and ac ...200919617386
evaluation of a biocontrol preparation consisting of enterobacter asburiae jx1 and a lytic bacteriophage cocktail to suppress the growth of salmonella javiana associated with tomatoes.a biocontrol preparation based on a combination of enterobacter asburiae jx1 and a cocktail of five lytic bacteriophages was evaluated for control of salmonella javiana within the rhizosphere of plants and in pre- and postharvest tomatoes. the biocontrol preparation introduced into the rhizosphere of growing tomato plants reduced the persistence of salmonella, although no synergistic action was observed between e. asburiae jx1 or the bacteriophage cocktail when used in combination. when the bioc ...200919903390
control of salmonella on sprouting mung bean and alfalfa seeds by using a biocontrol preparation based on antagonistic bacteria and lytic bacteriophages.the following reports on the application of a combination of antagonistic bacteria and lytic bacteriophages to control the growth of salmonella on sprouting mung beans and alfalfa seeds. antagonistic bacteria were isolated from mung bean sprouts and tomatoes by using the deferred plate assay to assess anti-salmonella activity. from the isolates screened, an enterobacter asburiae strain (labeled "jx1") exhibited stable antagonistic activity against a broad range of salmonella serovars (agona, ber ...201020051198
comparison of microbial diversity of edible flowers and basil grown with organic versus conventional methods.the consumption and use of edible flowers as food is growing; however, no study has been conducted to evaluate their role in the cause of food-borne illness or in food safety. recent food-borne outbreaks traced to fresh herbs have raised concern about their processing and handling. basil, one of the most commonly used fresh herbs, has been identified as a source of food-borne illness. baseline assessments of microflora were performed, and the microbial diversity between growing methods (organic ...201021076485
enterobacter mori sp. nov., a novel enterobacter species associated with bacterial wilt on morus alba l.two isolates of mulberry pathogenetic bacteria isolated from the diseased mulberry root were investigated by a polyphasic taxonomic study. comparative 16s rrna gene sequence analysis combined with rpob gene sequence analysis allocated the strain r18-2t and r3-3 to the genus enterobacter with enterobacter asburiae, e. aminigenus, e. cancerogenus, e. cloacae subsp. cloacae, e. cloacae subsp. dissolvens and e. nimipressuralis as closet relatives. the isolates are gram negative rod, facultative anae ...201121216919
a long-term low-frequency hospital outbreak of kpc-producing klebsiella pneumoniae involving intergenus plasmid diffusion and a persisting environmental reservoir.to study the molecular characteristics of a long-term, low frequency outbreak of bla kpc-2 in a low prevalence setting involving the hospital environment.201323536849
distribution of extended-spectrum β-lactamases, ampc β-lactamases, and carbapenemases among enterobacteriaceae isolates causing intra-abdominal infections in the asia-pacific region: results of the study for monitoring antimicrobial resistance trends (smart).the increasing trend of β-lactam resistance among enterobacteriaceae is a worldwide threat. enterobacteriaceae isolates causing intra-abdominal infections (iai) from the study for monitoring antimicrobial resistance trends (smart) collected in 2008 and 2009 from the asia-pacific region were investigated. detection of extended-spectrum β-lactamases (esbls), ampc β-lactamases, and carbapenemases was performed by multiplex pcr. a total of 699 enterobacteriaceae isolates with positive genotypic resu ...201323587958
complete genome sequence of a clinical isolate of enterobacter asburiae.we report here the complete genome sequence of enterobacter asburiae strain enipbj-cg1, isolated from a bone marrow transplant patient. the size of the genome sequence is approximately 4.65 mb, with a g+c content of 55.76%, and it is predicted to contain 4,790 protein-coding genes.201627284137
quorum sensing activity of enterobacter asburiae isolated from lettuce leaves.bacterial communication or quorum sensing (qs) is achieved via sensing of qs signaling molecules consisting of oligopeptides in gram-positive bacteria and n-acyl homoserine lactones (ahl) in most gram-negative bacteria. in this study, enterobacteriaceae isolates from batavia lettuce were screened for ahl production. enterobacter asburiae, identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (maldi-tof-ms) was found to produce short chain ahls. high resolutio ...201324152877
microbial diversity and dynamics during the production of may bryndza cheese.diversity and dynamics of microbial cultures were studied during the production of may bryndza cheese, a traditional slovak cheese produced from unpasteurized ewes' milk. quantitative culture-based data were obtained for lactobacilli, lactococci, total mesophilic aerobic counts, coliforms, e. coli, staphylococci, coagulase-positive staphylococci, yeasts, fungi and geotrichum spp. in ewes' milk, curd produced from it and ripened for 0 - 10 days, and in bryndza cheese produced from the curd, in th ...201424291178
genome analysis of quorum sensing cedecea neteri ssmd04 leads to identification of its novel signaling synthase (cnei), cognate receptor (cner) and an orphan receptor.cedecea neteri is a very rare human pathogen. we have isolated a strain of c. neteri ssmd04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. using the biosensor chromobacterium violaceum cv026, we have demonstrated the presence of short chain n-acyl-homoserine lactone (ahl) type quorum sensing (qs) activity in c. neteri ssmd04. triple quadrupole lc/ms analysis revealed that c. neteri ssmd04 produced short chain n-butyryl-homoserine lactone (c4-hsl). with the a ...201526355540
characterization of rhamnolipids produced by non-pathogenic acinetobacter and enterobacter bacteria.rhamnolipid production by two non-pathogenic bacterial strains acinetobacter calcoaceticus and enterobacter asburiae, and established rhamnolipid producer pseudomonas aeruginosa was investigated. rhamnolipids were separated from supernatant and further purified by thin-layer chromatography. mass spectrometry with negative electrospray ionization revealed rhamnolipid homologues varying in chain length and unsaturation. tandem mass spectrometry identified mono-rhamnolipid and di-rhamnolipid homolo ...201223313768
a dual approach employing maldi-tof ms and real-time pcr for fast species identification within the enterobacter cloacae complex.a real-time pcr procedure targeting the gene of the molecular cochaperon dnaj (dnaj) was developed for specific detection of strains belonging to the enterobacter cloacae group. the inclusivity and exclusivity of the real-time pcr assay were assessed with seven reference strains of e. cloacae, 12 other enterobacter species and 41 non-enterobacter strains. inclusivity as well as exclusivity of the duplex real-time pcr was 100%. in contrast, resolution of matrix-assisted laser desorption ionizatio ...201122150997
biomineralization and formulation of endosulfan degrading bacterial and fungal consortiums.microbial degradation offers an effective approach to remove toxicants and in this study, a microbial consortium consisting of bacterial strains and fungal strains were originally obtained from endosulfan contaminated agricultural soils. identification of the bacterial isolates by 16s rrna sequences revealed the isolates to be halophilic bacterium jas4, klebsiella pneumoniae jas8, enterobacter asburiae jas5, and enterobacter cloacae jas7, whereas the fungal isolates were identified by 18s rrna s ...201425454517
conservation and diversity of seed associated endophytes in zea across boundaries of evolution, ethnography and ecology.endophytes are non-pathogenic microbes living inside plants. we asked whether endophytic species were conserved in the agriculturally important plant genus zea as it became domesticated from its wild ancestors (teosinte) to modern maize (corn) and moved from mexico to canada. kernels from populations of four different teosintes and 10 different maize varieties were screened for endophytic bacteria by culturing, cloning and dna fingerprinting using terminal restriction fragment length polymorphis ...201121673982
plasmid load adversely affects growth and gluconic acid secretion ability of mineral phosphate-solubilizing rhizospheric bacterium enterobacter asburiae psi3 under p limited conditions.effect of the metabolic load caused by the presence of plasmids on mineral phosphate-solubilizing (mps) enterobacter asburiae psi3, was monitored with four plasmid cloning vectors and one native plasmid, varying in size, nature of the replicon, copy number and antibiotic resistance genes. except for one plasmid, the presence of all other plasmids in e. asburiae psi3 resulted in the loss of the mps phenotype as reflected by the failure to bring about a drop in ph and release soluble p when grown ...201120171856
genetic engineering of enterobacter asburiae strain jdr-1 for efficient d(--) lactic acid production from hemicellulose hydrolysate.in the dilute acid pretreatment of lignocellulose, xylose substituted with alpha-1,2-methylglucuronate is released as methylglucuronoxylose (megax), which cannot be fermented by biocatalysts currently used to produce biofuels and chemicals. enterobacter asburiae jdr-1, isolated from colonized wood, efficiently fermented both megax and xylose in acid hydrolysates of sweetgum xylan. deletion of pflb and als genes in this bacterium modified the native mixed acid fermentation pathways to one for hom ...200919504045
hydrogen production from formic acid in ph-stat fed-batch operation for direct supply to fuel cell.enterobacter asburiae snu-1 harvested after cultivation was used as a whole cell biocatalyst, for the production of hydrogen. formic acid was efficiently converted to hydrogen using the harvested cells with an initial hydrogen production rate and total hydrogen production of 491 ml/l/h and 6668 ml/l, respectively, when 1 g/l of whole cell enzyme was used. moreover, new ph-stat fed-batch operation was conducted, and total hydrogen production was 1.4 times higher than that of batch operation. for ...201019364642
bacterial communities on electron-beam pt-deposited electrodes in a mediator-less microbial fuel cell.the content of a bacterial consortium found on an electron beam (e-beam) pt-deposited electrode in a mediator-less microbial fuel cell (mfc) using glucose and glutamate as fuel is reported in this paper. the e-beam pt-deposited electrode and electrochemically active bacteria (eab) consortium were developed to improve the mediator-less mfc performance. denaturing gradient gel electrophoresis (dgge), restriction fragment length polymorphism (rflp), and 16s rrna sequencing were used to identify the ...200818767694
isolation and characterization of nonrhizobial plant growth promoting bacteria from nodules of kudzu (pueraria thunbergiana) and their effect on wheat seedling growth.the leguminous vine kudzu (pueraria thunbergiana) is an introduction into the n. w. himalayan region of india. despite its value as a fodder and cover crop, little is known about the nature of the nodule microflora. in an attempt to study the nodule bacteria, we isolated and characterized three nonrhizobial plant growth promoting bacteria from surface sterilized nodules of kudzu. based on the sequencing of the 16 s r rna gene, the isolates were designated as bacillus thuringiensis kr-1, enteroba ...200818074178
escherichia coli o157:h7 survival and growth on lettuce is altered by the presence of epiphytic bacteria.escherichia coli o157:h7 can survive in low numbers in soil and on plants. occasionally, conditions may occur in the field that lead to contamination of produce. survival of enteric pathogens in the field is controlled to a certain extent by complex interactions with indigenous soilborne and seedborne epiphytes. identifying these interactions may assist in developing strategies to improve produce safety. two epiphytes were isolated from pathogen-contaminated plants that interact differently with ...200617066909
substrate specificity of glucose dehydrogenase (gdh) of enterobacter asburiae psi3 and rock phosphate solubilization with gdh substrates as c sources.enterobacter asburiae psi3 is a rhizospheric isolate that solubilizes mineral phosphates by the action of a phosphate starvation-inducible gdh (ec 1.1.5.2). we report here that gdh activity of this isolate shows broad substrate range, being able to act on mono and disaccharides. enterobacter asburiae psi3 was proficient at bringing about a drop in ph and solubilization of rp with the use of 75 mmol/l of each of the gdh substrate sugars tested as the sole c source. it liberated amounts of p rangi ...200516121225
carbapenemase-producing enterobacteriaceae, u.s. rivers.our study was initiated by previous isolation of 30 imipenem-resistant, gram-negative rods from 7 of 16 u.s. rivers sampled from 1999 to 2001. imipenem hydrolysis was detected in 22 of those isolates identified as enterobacter asburiae. random amplified polymorphism dna analysis showed that these e. asburiae isolates were genetically indistinguishable. an identical clavulanic acid-inhibited beta-lactamase imi-2 was identified from each isolate that shared 99% and 97% amino acid identity with the ...200515752444
kinetic characterization of a novel acid ectophosphatase from enterobacter asburiae.expression of acid ectophosphatase by enterobacter asburiae, isolated from cattleya walkeriana (orchidaceae) roots and identified by the 16s rrna gene sequencing analysis, was strictly regulated by phosphorus ions, with its optimal activity being observed at an inorganic phosphate concentration of 7 mm. at the optimum ph 3.5, intact cells released p-nitrophenol at a rate of 350.76 ± 13.53 nmol of p-nitrophenolate (pnp)/min/10(8) cells. the membrane-bound enzyme was obtained by centrifugation at ...201626832666
effect of antimicrobial peptides on colistin-susceptible and colistin-resistant strains of klebsiella pneumoniae and enterobacter asburiae.in this study susceptibility to different antimicrobial peptides was investigated on colistin-susceptible and colistin-resistant identical pulsotype strains of kpc-2 producing klebsiella pneumoniae st258 as well as colistin-susceptible and colistin-resistant enterobacter asburiae strains isolated from clinical samples. in our test, bacteria were exposed to 50 mg/ml lactoferrin, lysozyme and protamine - cationic antimicrobial peptides belonging to innate immune system and having structural simila ...201526689883
enterobacter muelleri sp. nov., isolated from the rhizosphere of zea mays.a beige-pigmented, oxidase-negative bacterial strain (jm-458t), isolated from a rhizosphere sample, was studied using a polyphasic taxonomic approach. cells of the isolate were rod-shaped and stained gram-negative. a comparison of the 16s rrna gene sequence of strain jm-458t with sequences of the type strains of closely related species of the genus enterobacter showed that it shared highest sequence similarity with enterobacter mori (98.7 %), enterobacter hormaechei (98.3 %), enterobacter cloaca ...201526294947
microbiological diversity associated with the spontaneous wet method of coffee fermentation.the evaluation of the microbiota present during coffee wet fermentation was done in two distinct regions of minas gerais, brazil: one farm in the south of minas gerais (lavras=l) and another farm in the savannah region (monte carmelo=mc). the yeast population ranged from 2.48 to 4.92 log cfu/g and from 2 to 4.81 log cfu/g, the mesophilic bacteria population ranged from 3.83 to 8.47 log cfu/g and from 5.37 to 7.36 log cfu/g, and the lab population ranged from 2.57 to 5.66 log cfu/g and from 3.40 ...201526119187
biodegradation of chlorpyrifos by enterobacter strain b-14 and its use in bioremediation of contaminated soils.six chlorpyrifos-degrading bacteria were isolated from an australian soil and compared by biochemical and molecular methods. the isolates were indistinguishable, and one (strain b-14) was selected for further analysis. this strain showed greatest similarity to members of the order enterobacteriales and was closest to members of the enterobacter asburiae group. the ability of the strain to mineralize chlorpyrifos was investigated under different culture conditions, and the strain utilized chlorpy ...200415294824
population genetics of the nomenspecies enterobacter cloacae.the genetic heterogeneity of the nomenspecies enterobacter cloacae is well known. enterobacter asburiae, enterobacter cancerogenus, enterobacter dissolvens, enterobacter hormaechei, enterobacter kobei, and enterobacter nimipressuralis are closely related to it and are subsumed in the so-called e. cloacae complex. dna-dna hybridization studies performed previously identified at least five dna-relatedness groups of this complex. in order to analyze the genetic structure and the phylogenetic relati ...200312957918
evidence of polyethylene biodegradation by bacterial strains from the guts of plastic-eating waxworms.polyethylene (pe) has been considered nonbiodegradable for decades. although the biodegradation of pe by bacterial cultures has been occasionally described, valid evidence of pe biodegradation has remained limited in the literature. we found that waxworms, or indian mealmoths (the larvae of plodia interpunctella), were capable of chewing and eating pe films. two bacterial strains capable of degrading pe were isolated from this worm's gut, enterobacter asburiae yt1 and bacillus sp. yp1. over a 28 ...201425384056
expansion of esbl-producing klebsiella pneumoniae in hospitalized patients: a successful story of international clones (st15, st147, st336) and epidemic plasmids (incr, incfiik).the aim of this study was to characterize by a multi-level approach extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae isolates other than e. coli from portuguese hospitals. eighty-eight esbl-producing clinical isolates (69 klebsiella pneumoniae, 13 enterobacter cloacae complex, 3 klebsiella oxytoca, 1 enterobacter asburiae, 1 proteus mirabilis and 1 serratia marcescens) recovered from hospitals located in the north (a) or centre (b, c) regions during two time periods (2006-7 and ...201425190354
halotolerance and survival kinetics of lactic acid bacteria isolated from jalapeño pepper (capsicum annuum l.) fermentation.the microbiota associated with spontaneous fermentation of vegetables in a saline substrate may represent an important group of bacteria in the food industry. in this work, the lactic acid bacteria (lab) weissella cibaria, lactobacillus plantarum, lactobacillus paraplantarum, and leuconostoc citreum were identified by partial 16s rrna gene sequence analysis. in addition, entophytic bacteria such as pantoea eucalypti, pantoea anthophila, enterobacter cowanii, and enterobacter asburiae were detect ...201425039289
enterobacter xiangfangensis sp. nov., isolated from chinese traditional sourdough, and reclassification of enterobacter sacchari zhu et al. 2013 as kosakonia sacchari comb. nov.a gram-stain-negative bacterial strain, 10-17(t), was isolated from traditional sourdough in heilongjiang province, china. the bacterium was characterized by a polyphasic approach, including 16s rrna gene sequence analysis, rna polymerase β subunit (rpob) gene sequence analysis, dna gyrase (gyrb) gene sequence analysis, initiation translation factor 2 (infb) gene sequence analysis, atp synthase β subunit (atpd) gene sequence analysis, fatty acid methyl ester analysis, determination of dna g+c co ...201424824638
carbapenemase-producing enterobacteriaceae in a tertiary hospital in madrid, spain: high percentage of colistin resistance among vim-1-producing klebsiella pneumoniae st11 isolates.here we describe the carbapenemase genes, genetic relatedness and antimicrobial susceptibility data of 123 carbapenemase-producing enterobacteriaceae (cpe) clinical isolates recovered from 2010 to 2012, comprising klebsiella pneumoniae (n = 79), klebsiella oxytoca (n = 13), serratia marcescens (n = 14), enterobacter cloacae (n = 12), enterobacter asburiae (n = 4) and enterobacter aerogenes (n = 1). vim-1 was the most common carbapenemase (n = 101) followed by kpc-2 (n = 19), oxa-48 (n = 2) and i ...201424657043
isolation, screening, characterization, and selection of superior rhizobacterial strains as bioinoculants for seedling emergence and growth promotion of mandarin orange (citrus reticulata blanco).mandarin orange (mo) is an important fruit crop of tropical and subtropical regions of the world. a total of 217 morphologically distinct rhizobacteria from mo orchards in 3 states of northeastern india were isolated and analyzed for 4 plant-growth-promoting (pgp) attributes: nitrogen fixation, production of indole acetic acid like substances, solubilization of phosphate, and ability to antagonize pathogenic fungi. isolates were ranked based on in-vitro-assayed pgp attributes, and 10 superior is ...201424498985
community-acquired pneumonia caused by enterobacter asburiae. 200111460856
identification by 16s ribosomal rna gene sequencing of an enterobacteriaceae species with ambiguous biochemical profile from a renal transplant recipient.traditional ways of identification of bacteria by phenotypic characteristics cannot be used for non-cultivable organisms and organisms with unusual biochemical profiles. in this study, an enterobacteriaceae was isolated in pure growth from the mid-stream urine of a 67-year old renal transplant recipient with urinary tract infection. conventional biochemical tests did not reveal a pattern resembling any known member of the enterobacteriaceae family. the vitek system (gni+) showed that it was 18% ...200111248520
[species of enterobacteria new to medicine].the paper presents main biological characteristics of six new species of enterobacteria, which may cause a number of infectious processes in man: moellerella wisconsensis, koserella trabulsii. leclercia adecarboxylata. escherichia fergusonii. rahnella aquatilis, enterobacter asburiae. the characteristics presented allow the bacteriologists of the laboratory of health services to isolate and to identify the above-mentioned enterobacteria.19969044705
enterobacter cloacae complex: clinical impact and emerging antibiotic resistance.species of the enterobacter cloacae complex are widely encountered in nature, but they can act as pathogens. the biochemical and molecular studies on e. cloacae have shown genomic heterogeneity, comprising six species: enterobacter cloacae, enterobacter asburiae, enterobacter hormaechei, enterobacter kobei, enterobacter ludwigii and enterobacter nimipressuralis, e. cloacae and e. hormaechei are the most frequently isolated in human clinical specimens. phenotypic identification of all species bel ...201222827309
spread of an ompk36-modified st15 klebsiella pneumoniae variant during an outbreak involving multiple carbapenem-resistant enterobacteriaceae species and clones.we aim to characterise multiple ertapenem-resistant (ert-r, n = 15) enterobacteriaceae isolates identified as presumptive carbapenemase producers in a portuguese hospital in a short period of time (march-july 2010). antibiotic susceptibility patterns, β-lactamases, genetic relatedness [pulsed-field gel electrophoresis (pfge), multi-locus sequence typing (mlst)], plasmid content and major enterobacterial porins were investigated. ertapenem resistance was associated with deficiencies in major pori ...201222706513
enterobacter asburiae sp. nov., a new species found in clinical specimens, and reassignment of erwinia dissolvens and erwinia nimipressuralis to the genus enterobacter as enterobacter dissolvens comb. nov. and enterobacter nimipressuralis comb. nov.enterobacter asburiae sp. nov. is a new species that was formerly referred to as enteric group 17 and that consists of 71 strains, 70 of which were isolated from humans. enterobacter asburiae sp. nov. strains gave positive reactions in tests for methyl red, citrate utilization (simmons and christensen's), urea hydrolysis, l-ornithine decarboxylase, growth in kcn, acid and gas production from d-glucose, and acid production from l-arabinose, cellobiose, glycerol (negative in 1 to 2 days, positive ...19863711302
[new enterobacteriaceae found in medical bacteriology moellerella wisconsensis, koserella trabulsii, leclercia adecarboxylata, escherichia fergusonii, enterobacter asbutiae, rahnella aquatilis].characteristics of 6 "new" enterobacteriaceae species, occasionally isolated from human sources: moellerella wisconsensis, koserella trabulsii, leclercia adecarboxylata, escherichia fergusonii, rahnella aquatilis, enterobacter asburiae are described and compared with those of closely related species.19892742218
bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria.seventy-five bacterial contaminants which still persisted to cleaning system from three puff pastry production lines (dough forming, layer and filling forming, and shock freezing) were identified using 16s rdna as seven genera of bacillus, corynebacterium, dermacoccus, enterobacter, klebsiella, pseudomonas, and staphylococcus with detection frequencies of 24.00, 2.66, 1.33, 37.33, 1.33, 2.66, and 30.66, respectively. seventeen species were discovered while only 11 species bacillus cereus, b. sub ...201728572930
structural and physiochemical characterization of rhamnolipids produced by acinetobacter calcoaceticus, enterobacter asburiae and pseudomonas aeruginosa in single strain and mixed cultures.rhamnolipids are naturally occurring biosurfactants with a wide range of potential commercial applications. as naturally derived products they present an ecological alternative to synthetic surfactants. the majority of described rhamnolipid productions are single strain pseudomonas spp. cultivations. here we report rhamnolipids producing bacteria acinetobacter calcoaceticus, enterobacter asburiae and pseudomonas aeruginosa that were cultivated separately and as mixed populations. the ratio and c ...201525433178
colistin resistance associated with outer membrane protein change in klebsiella pneumoniae and enterobacter asburiae.in this study, outer membrane proteins (omps) of colistin-resistant klebsiella pneumoniae and enterobacter asburiae were analyzed. one colistin-susceptible and three colistin-resistant k. pneumoniae sequence type 258 strains as well as one colistin-susceptible e. asburiae and its colistin-heteroresistant counterpart strain were involved in the study. omp analysis of each strain was performed by microchip method. matrix-assisted laser desorption ionization time of flight/mass spectrometry (maldi- ...201728629229
new insight into microbial diversity and functions in traditional vietnamese alcoholic fermentation.the roles of microorganisms in traditional alcoholic fermentation are often assumed based on abundance in the starter and activity in pure culture. there is a serious lack of hard evidence on the behavior and activity of individual microbial species during the actual fermentation process. in this study, microbial succession and metabolite changes during 7days of traditional vietnamese alcoholic fermentation were monitored. special attention was devoted to starch degradation. in total, 22 microbi ...201627236224
enterobacter asburiae strain l1: complete genome and whole genome optical mapping analysis of a quorum sensing bacterium.enterobacter asburiae l1 is a quorum sensing bacterium isolated from lettuce leaves. in this study, for the first time, the complete genome of e. asburiae l1 was sequenced using the single molecule real time sequencer (pacbio rsii) and the whole genome sequence was verified by using optical genome mapping (opgen) technology. in our previous study, e. asburiae l1 has been reported to produce ahls, suggesting the possibility of virulence factor regulation which is quorum sensing dependent. this ev ...201425196111
draft genome sequence of endophytic bacterium enterobacter asburiae pda134, isolated from date palm (phoenix dactylifera l.) roots.in this report, a draft of the enterobacter asburiae strain pda134 genome was sequenced. this bacterial strain was isolated from the root tissue of a date palm, where it has the ability to produce 1-aminocyclopropane-1-carboxylic acid (acc) deaminase and indole-3-acetic acid (iaa) under salinity stress.201627540071
characterization of a novel putative xer-dependent integrative mobile element carrying the bla(nmc-a) carbapenemase gene, inserted into the chromosome of members of the enterobacter cloacae complex.an enterobacter ludwigii strain was isolated during routine screening of a japanese patient for carriage of carbapenem-resistant enterobacteriaceae. pcr analysis revealed the blanmc-a carbapenemase gene. whole-genome sequencing revealed that blanmc-a was inserted in the chromosome and associated with a novel 29.1-kb putative xer-dependent integrative mobile element, named eludimex-1. bioinformatic analysis identified similar elements in the genomes of an enterobacter asburiae strain and of other ...201526248383
alterations and correlations of the gut microbiome, metabolism and immunity in patients with primary biliary cirrhosis.we selected 42 early-stage primary biliary cirrhosis (pbc) patients and 30 healthy controls (hc). metagenomic sequencing of the 16s rrna gene was used to characterize the fecal microbiome. uplc-ms/ms assaying of small molecules was used to characterize the metabolomes of the serum, urine and feces. liquid chip assaying of serum cytokines was used to characterize the immune profiles. the gut of pbc patients were depleted of some potentially beneficial bacteria, such as acidobacteria, lachnobacter ...201627243236
enterobacter asburiae and aeromonas hydrophila: soft tissue infection requiring debridement.enterobacter asburiae and aeromonas hydrophila are gram-negative bacilli that have been isolated in soil and water. enterobacter asburiae can cause an array of diseases, and exposure to a hydrophila can cause soft tissue infections, including necrotizing faciitis.a healthy-appearing 22-year-old man presented with an innocuous soft tissue injury to his leg due to an all-terrain vehicle crash. he received intravenous antibiotics and was discharged with prophylactic oral antibiotics. after the rapi ...201222691684
characterization of microbial population of breba and main crops (ficus carica) during cold storage: influence of passive modified atmospheres (map) and antimicrobial extract application.the purpose of this work was to study the changes of bacterial and fungal population of breba fruits such as 'banane' and 'san antonio' as well as 'cuello dama negro', 'cuello dama blanco' and 'san antonio' fig cultivars stored in passive modified atmospheres (map) by the use of three different microperforated films (m10 with 16 holes; m30 with five holes and m50 with three holes). moreover the effects of the application of aqueous soy polyphenolic antimicrobial extract (ape), alone or combined ...201728040180
degradation of acephate by enterobacter asburiae, bacillus cereus and pantoea agglomerans isolated from diamondback moth plutella xylostella (l), a pest of cruciferous crops.acephate-degrading bacterial isolates were isolated from the larval gut of diamondback moth plutella xylostella, a notorious pest of cruciferous crops worldwide that has developed resistance to insecticides. partial 16s rrna gene sequencing identified the isolates as bacillus cereus (px-b.c.or), enterobacter asburiae (pxe), and pantoae agglomerans (px-pt.ag.jor). all isolates grew on minimal media (mm) in the presence of acephate at 100 and 200 ppm, with maximum growth at 200 ppm. lc-ms analyses ...201627498509
quorum quenching activity in cell-free lysate of endophytic bacteria isolated from pterocarpus santalinus linn., and its effect on quorum sensing regulated biofilm in pseudomonas aeruginosa pao1.quorum sensing mechanism allows the microorganisms to resist the antibiotic treatment by forming biofilms. quorum quenching is one of the mechanisms to control the development of drug resistance in microbes. endophyte bacteria are beneficial to plant growth as they support the immune system against the pathogen attack. the endophytic bacteria present in pterocarpus santalinus were screened for the presence of n-acyl homoserine lactones (ahls) degrading bacteria using biosensor strains and furthe ...201424268182
international life science institute north america cronobacter (formerly enterobacter sakazakii) isolate set.foodborne pathogen isolate collections are important for the development of detection methods, for validation of intervention strategies, and to develop an understanding of pathogenesis and virulence. we have assembled a publicly available cronobacter (formerly enterobacter sakazakii) isolate set that consists of (i) 25 cronobacter sakazakii isolates, (ii) two cronobacter malonaticus isolates, (iii) one cronobacter muytjensii isolate, which displays some atypical phenotypic characteristics, bioc ...201323317855
in vitro antibacterial activity of mgdg-palmitoyl from oscillatoria acuminata ntapc05 against extended-spectrum β-lactamase producers.extended-spectrum β-lactamase (esbl)-producing bacteria pose a big challenge in clinical practices, warranting a new therapeutic strategy. in this study, methanol extract of the marine cyanobacterium oscillatoria acuminata ntapc05 was fractionated under bioassay guidance and the fractions were tested against three well-characterized esbl-producing bacteria escherichia coli u655, stenotrophomonas maltophilia b929 and enterobacter asburiae b938. out of the four hplc fractions, fraction 2 showed ba ...201728377637
seed vectored endophytic bacteria modulate development of rice seedlings.the aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens.201728375579
high-quality genome sequence of human pathogen enterobacter asburiae type strain 1497-78(t).enterobacter asburiae belongs to the enterobacter cloacae complex (ecc), which comprises six heterogenic species. these bacteria can cause nosocomial infections. moreover, they are well known for antibiotic resistance features based on overproduction of ampc β-lactamases. although ecc have clinical importance, little is known about their virulence-associated properties, and very few strains from the six species have been sequenced. in this study, the type strain of e. asburiae 1497-78(t) (atcc 3 ...201728082144
sucrose dependent mineral phosphate solubilization in enterobacter asburiae psi3 by heterologous overexpression of periplasmic invertases.enterobacter asburiae psi3 solubilizes mineral phosphates in the presence of glucose by the secretion of gluconic acid generated by the action of a periplasmic pyrroloquinoline quinone dependent glucose dehydrogenase. in order to achieve mineral phosphate solubilization phenotype in the presence of sucrose, plasmids pcnk4 and pcnk5 containing genes encoding the invertase enzyme of zymomonas mobilis (invb) and of saccharomyces cerevisiae (suc2) under constitutive promoters were constructed with m ...201627722827
enterobacter asburiae kuni5, a nickel resistant bacterium for possible bioremediation of nickel contaminated sites.nickel resistant bacterial strain enterobacter asburiae kuni5 was isolated and showed resistance up to 15 mm and could remove ni optimally better at 37 degrees c and ph 7. maximum removal was found at initial concentration of 0.5 to 2 mm, however, growth and ni removal were affected by other heavy metals. major amount of the metal was accumulated in the membrane fractions and certain negatively charged groups were found responsible for ni binding. kuni5 could also produce 1-aminocyclopropane-1-c ...201627282003
isolation and identification enterobacter asburiae from consumed powdered infant formula milk (pif) in the neonatal intensive care unit (nicu).enterobacter asburiae (e. asburiae) is a facultative anaerobic, non-spore-forming gram-negative rod-shaped bacterium belonging to the family of enterobacteriaceae. it is an opportunistic pathogen that its strains are isolated from a variety of clinical and environmental specimens. since powdered infant formula milk (pif) is not a sterile product, it is an excellent medium for bacterial growth. the aim of this study was to isolate and identify e. asburiae from pif in the neonatal intensive care u ...201626853289
enterobacter asburiae ke17 association regulates physiological changes and mitigates the toxic effects of heavy metals in soybean.this study aimed to elucidate the role played by enterobacter asburiae ke17 in the growth and metabolism of soybeans during copper (100 μm cu) and zinc (100 μm zn) toxicity. when compared to controls, plants grown under cu and zn stress exhibited significantly lower growth rates, but inoculation with e. asburiae ke17 increased growth rates of stressed plants. the concentrations of plant hormones (abscisic acid and salicylic acid) and rates of lipid peroxidation were higher in plants under heavy ...201525940948
plant growth promoting bacteria enterobacter asburiae jas5 and enterobacter cloacae jas7 in mineralization of endosulfan.endosulfan and their metabolites can be detected in soils with a history of endosulfan application. microbial degradation offers an effective approach to remove toxicants, and in this study, enterobacter asburiae jas5 and enterobacter cloacae jas7 were isolated through enrichment technique. the biodegradation of endosulfan and its metabolites rate constant (k) and dt50 were determined through first-order kinetic models. e. asburiae jas5 degraded the endosulfan, and its metabolites in liquid medi ...201525638268
molecular identification of aiia homologous gene from endophytic enterobacter species and in silico analysis of putative tertiary structure of ahl-lactonase.the aiia homologous gene known to encode ahl- lactonase enzyme which hydrolyze the n-acylhomoserine lactone (ahl) quorum sensing signaling molecules produced by gram negative bacteria. in this study, the degradation of ahl molecules was determined by cell-free lysate of endophytic enterobacter species. the percentage of quorum quenching was confirmed and quantified by hplc method (p<0.0001). amplification and sequence blast analysis showed the presence of aiia homologous gene in endophytic enter ...201424309109
first report of imi-1-producing colistin-resistant enterobacter clinical isolate in ireland, march 2013.we report the first case in ireland of an imi-1 carbapenemase-producing enterobacter asburiae, which was resistant to both colistin and fosfomycin. the circumstances under which this isolate was acquired were unclear. several reports of imi-producing enterobacter spp. have emerged in recent years, and colistin resistance in enterobacteriaceae is also increasingly reported. laboratories should be aware of the unusual antibiograms of imi-producing isolates.201323929227
2-ketogluconic acid secretion by incorporation of pseudomonas putida kt 2440 gluconate dehydrogenase (gad) operon in enterobacter asburiae psi3 improves mineral phosphate solubilization.enterobacter asburiae psi3 is known to efficiently solubilize rock phosphate by secretion of approximately 50 mm gluconic acid in tris-buffered medium in the presence of 75 mm glucose and in a mixture of seven aldosugars each at 15 mm concentration, mimicking alkaline vertisol soils. efficacy of this bacterium in the rhizosphere requires p release in the presence of low amount of sugars. to achieve this, e. asburiae psi3 has been manipulated to express gluconate dehydrogenase (gad) operon of pse ...201323666029
isolation and characterization of plant growth promoting bacteria from non-rhizospheric soil and their effect on cowpea (vigna unguiculata (l.) walp.) seedling growth.the efficacy of four potential phosphate solubilizing enterobacter isolated from non-rhizospheric soil in western ghat forest in india. plant growth promoting ability of these isolates was evaluated in cowpea. all are gram negative, rod shaped, 0.8-1.6 mm in size, and psychrotrophic in nature, grow from 5 to 40°c (optimum temp. 28 ± 2°c). all isolates exhibits growth at a wide range of ph 6-12, optimum at ph 7.0 and tolerates up to 7% (w/v) salt concentration. 16s rrna gene sequencing reveals th ...201024026928
glucose dehydrogenase of a rhizobacterial strain of enterobacter asburiae involved in mineral phosphate solubilization shares properties and sequence homology with other members of enterobacteriaceae.glucose dehydrogenase (gdh) of gram-negative bacteria is a membrane bound enzyme catalyzing the oxidation of glucose to gluconic acid and is involved in the solubilization of insoluble mineral phosphate complexes. a 2.4 kb glucose dehydrogenase gene (gcd) of enterobacter asburiae sharing extensive homology to the gcd of other enterobacteriaceae members was cloned in a pcr-based directional genome walking approach and the expression confirmed in escherichia coli yu423 on both macconkey glucose ag ...200723100654
metagenomic sequencing of diamondback moth gut microbiome unveils key holobiont adaptations for herbivory.herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. our previous study of the genome of diamondback moth, plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. here we expand on that work with a complete taxonomic and functi ...201728491055
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