| pathogenic amoebae in natural thermal waters of three resorts of hidalgo, mexico. | in a search for free-living amoebae, seven water samples from three thermal water bathing resorts in tecozautla, hidalgo, were analyzed during december 1984. the samples were concentrated by filtration and centrifugation, and inoculated later on monoxenic and axenic media. the identification of the isolates was performed by morphology and isoelectric focusing of isoenzymes and total proteins. thirty-three strains of free-living amoebae belonging to the genera naegleria, acanthamoeba, and willaer ... | 1989 | 2583075 |
| [acanthamoeba keratitis. epidemiologic and parasitologic study]. | the amebic keratitis with acanthamoeba are until now rare, but however increasing as their diagnosis is better, and the contact lens wearers more numerous. the authors present the clinical and parasitological features of cases they did diagnose since 1986. in the immunological sphere, the t4 lymphocytes were 20% less than the normal for the first patient, and the third one had a very low level of immunoglobulins a. in the evolution sphere, the first one did received a keratoplasty; the second on ... | 1989 | 2621311 |
| effects of muramyl dipeptide and trehalose dimycolate on resistance of mice to toxoplasma gondii and acanthamoeba culbertsoni infections. | the effects of synthetic muramyl dipeptide (mdp) and natural trehalose dimycolate (tdm) against parasitic infections by intracellular toxoplasma gondii and free-living acanthamoeba culbertsoni were studied. significant resistance against oral t. gondii infection was induced by intraperitoneal pretreatment with tdm but not with mdp. the protective effect of tdm against t. gondii was corroborated by a significant reduction in the number of cysts in brains of pretreated animals and elevated serum a ... | 1986 | 3759255 |
| acridine orange staining for rapid diagnosis of acanthamoeba keratitis. | acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. therefore, accurate and rapid diagnosis of acanthamoeba keratitis is essential for successful treatment and good prognosis. we evaluated the usefulness of acridine orange staining from corneal scrapings and contact lens solutions for the rapid diagnosis of four ... | 1998 | 9587842 |
| [acanthamoeba keratitis after use of soft contact lenses--case report]. | the case history of a 39-year patient suffering from a deep inflammation of cornea and not responding to conventional antibiotic treatment is presented. the patient was using soft contact lenses during the period of initial symptoms; moreover, he was bathing in thermal bathing pool. a cultivation examination of smears from the area of corneal defect revealed the presence of acanthamoeba lugdunensis in combination with bacterial infection by pseudomonas aeruginosa. the available data indicate tha ... | 2003 | 14518364 |
| dialkylamino and nitrogen heterocyclic analogues of hexadecylphosphocholine and cetyltrimetylammonium bromide: effect of phosphate group and environment of the ammonium cation on their biological activity. | a series of dialkylamino and nitrogen heterocyclic analogues of hexadecylphosphocholine and cetyltrimethylammonium bromide have been synthesized. the prepared compounds exhibit significant cytotoxic, antifungal and antiprotozoal activities. alkylphosphocholines possess higher antifungal activity against candida albicans in comparison with quaternary ammonium compounds. however, quaternary ammonium compounds exhibit significant higher activity against human tumor cells and acanthamoeba lugdunensi ... | 2009 | 19762125 |
| synthesis and biological activity of dialkylphosphocholines. | a series of dialkylphosphocholines were prepared and evaluated for their biological activity. the antiprotozoal activity was determined against acanthamoeba lugdunensis. compound 15 exhibited excellent trophocidal activity. none of the tested dialkylphosphocholines exhibited better fungicidal activity against candida albicans than miltefosine. the antineoplastic activity was determined against hela. the most cytotoxic was compound 10, which was more active against tumor cells as against normal c ... | 2009 | 19818608 |
| weak cytotoxic activity of miltefosine against clinical isolates of acanthamoeba spp. | abstract hexadecylphosphocholine (miltefosine) is an anticancer drug active in vitro against various protozoan parasites, and recently used for the treatment of disseminated acanthamoeba infection. in the present study, we present results of weak cytotoxic activity of this potential amoebicidal agent for 2 of 3 clinical isolates of acanthamoeba spp. although the inhibition effect for all tested concentrations was apparent, and showed 100% eradication of trophozoites of acanthamoeba castellanii s ... | 2010 | 21506779 |
| Relationship between aggregation properties and antimicrobial activities of alkylphosphocholines with branched alkyl chains. | Synthesis of five alkylphosphocholines with branched alkyl chains (Isophol-PCs) with different length of alkyl chains was described. Isophol(8)-PC and Isophol(12)-PC represent new compounds. The physico-chemical properties of Isophol-PCs were determined, critical micelle concentration and types of formed aggregates in aqueous solutions were investigated. The biological activities of Isophol-PCs have been studied for the first time in the present study. Antimicrobial activities of alkylphosphocho ... | 2011 | 22178895 |
| synthesis of structural analogues of hexadecylphosphocholine and their antineoplastic, antimicrobial and amoebicidal activity. | twelve derivatives of hexadecylphosphocholine (miltefosine) were synthesized to determine how the position and length of the alkyl chain within the molecule influence their biological activities. the prepared alkylphosphocholines have the same molecular formula as miltefosine. activity of the compounds was studied against a spectrum of tumour cells, two species of protozoans, bacteria and yeast. antitumour efficacy of some alkylphosphocholines measured up on mcf-7, a2780, hut-78 and thp-1 cell l ... | 2015 | 25698517 |
| synthesis, self-aggregation and biological properties of alkylphosphocholine and alkylphosphohomocholine derivatives of cetyltrimethylammonium bromide, cetylpyridinium bromide, benzalkonium bromide (c16) and benzethonium chloride. | a series of alkylphosphocholine and alkylphosphohomocholine derivatives of cetyltrimethylammonium bromide, cetylpyridinium bromide, benzalkonium bromide (c16) and benzethonium chloride have been synthesized. their physicochemical properties were also investigated. the critical micelle concentration (cmc), the surface tension value at the cmc (γcmc), and the surface area at the surface saturation per head group (acmc) were determined by means of surface tension measurements. the prepared compound ... | 2013 | 23792315 |
| adhesion of acanthamoeba on cosmetic contact lenses. | this study aimed to evaluate the adhesion of acanthamoeba trophozoites on cosmetic contact lenses (cls) with and without cl care multipurpose solution (mps) treatment. | 2018 | 29318793 |
| adhesion of acanthamoeba on silicone hydrogel contact lenses. | to evaluate adhesion of acanthamoeba trophozoites to different silicone hydrogel contact lens (shcl) generations with and without multipurpose contact lens care solution (mps) treatment. | 2016 | 26938330 |
| laboratory investigation of acanthamoeba lugdunensis from patients with keratitis. | species of four acanthamoeba isolates (ka/e2, ka/e12, ka/e15, and ka/e16) from the cornea of patients with keratitis were identified and their molecular characteristics compared with those of other strains. | 2004 | 15111597 |
| purification and characterization of a 33 kda serine protease from acanthamoeba lugdunensis ka/e2 isolated from a korean keratitis patient. | in order to evaluate the possible roles of secretory proteases in the pathogenesis of amoebic keratitis, we purified and characterized a serine protease secreted by acanthamoeba lugdunensis ka/e2, isolated from a korean keratitis patient. the ammonium sulfate-precipitated culture supernatant of the isolate was purified by sequential chromatography on cm-sepharose, sephacryl s-200, and mono q-anion exchange column. the purified 33 kda protease had a ph optimum of 8.5 and a temperature optimum of ... | 2003 | 14699259 |
| [bacterial endosymbiosis within the cytoplasm of acanthamoeba lugdunensis isolated from a contact lens storage case]. | transmission electron microscopy of an acanthamoeba isolate (ka/l5) from a contact lens case revealed bacterial endosymbionts within cytoplasm of the amoebae. the acanthamoeba isolate belonged to the morphological group ii. based on the polymerase chain reaction (pcr)-restriction fragment length polymorphism (rflp) of 18s ribosomal rna coding dna (rdna), the isolate was identified as a. lugdunensis. strain typing by isoenzyme analysis using isoelectric focusing (ief) and mitochondrial (mt) dna r ... | 1997 | 9241987 |