| its-2 rdna sequencing of gnathostoma species (nematoda) and elucidation of the species causing human gnathostomiasis in the americas. | from several gnathostome species the complete internal transcribed spacer its-2 ribosomal dna (rdna) repeat sequence and a fragment of the 5.8s rdna were obtained by direct polymerase chain reaction cycle-sequencing and silver-staining methods. the size of the complete its-1 sequence in agarose gel electrophoresis was also obtained. the its-2 enabled the differentiation of gnathostoma spinigerum from thailand and gnathostoma binucleatum from mexico and ecuador and confirmed the validity of the l ... | 2000 | 10864252 |
| morphology of gnathostoma spp. isolated from natural hosts in sinaloa, mexico. | gnathostomosis is an emerging public health problem in sinaloa, mexico, where an increasing number of human cases have been diagnosed since 1989. the present study was carried out to determine the presence of the parasite in other natural hosts from the area. birds, fish, opossums and raccoons were captured from local dams and lagoons. the flesh from bird and fish specimens was ground and examined under a 100 w light bulb. larvae were processed for light and electron microscopy. a total of 368 a ... | 2002 | 12107456 |
| study of the ethiological agent of gnathostomosis in nayarit, mexico. | in order to clarify the specific identity of the etiological agent of human gnathostomosis in nayarit state, mexico, morphological and molecular studies were conducted on advanced third stage larvae obtained from human and fish tissue. cathorops fuerthii from agua brava lagoons complex, was the only fish species found to be infected among four species surveyed. morphological variability does not allow specific identification of the larvae. internal transcribed spacer 2 of the ribosomal dna was s ... | 2002 | 12113759 |
| gnathostoma binucleatum: excretion-secretion antigen analysis obtained from advanced third-stage larvae in in vitro culture. | the paper describes an introductory characterization of antigenic stimulation of excretion-secretion products (esp) of gnathostoma binucleatum advanced third-stage larvae cultured in vitro and proteinases present in this products. excretory and secretory proteins were obtained after 10 larvae were maintained in 5% co(2) rpmi medium. the supernatant was collected each week for two months. the proteins were dialyzed, concentrated, and separated in 10% sds-page gels under reducing conditions and tr ... | 2005 | 15888296 |
| a new species of gnathostoma (nematoda: gnathostomatidae) in procyon lotor hernandezii from mexico. | gnathostoma lamothei n. sp., inhabiting the stomach of procyon lotor hernandezii wagler, 1831, in tlacotalpan, veracruz state, and rio sapo, oaxaca, mexico, is described. this new species differs from all other congeners by having the posterior half of the body surface covered by rows of tiny round bosses instead of spines, or lacking ornamentations. sequences of the its2 of the ribosomal dna of g. lamothei n. sp. are compared with sequences of other species of the genus recorded in mexico; they ... | 2005 | 16419762 |
| confirmation of gnathostoma binucleatum almeyda-artigas, 1991, advanced third-stage larvae in tres palos lagoon, mexico, by morphological and molecular data. | advanced third-stage larvae of gnathostoma sp. corresponding to 6 morphotypes, distinguished on the basis of the shape of the cephalic hooklets, were collected from the muscle tissue of 5 species of freshwater fish (i.e., dormitator latifrons, eleotris picta, gobiomorus maculatus, ariopsis guatemalensis, and cichlasoma trimaculatum) in tres palos lagoon, guerrero, mexico. principal components analysis of 4 morphological characters cluster all samples in a single taxonomic group. a minimum amount ... | 2005 | 17089777 |
| protein profile analysis from advanced third-stage larvae (advl3) and adult worms of gnathostoma binucleatum (nematoda: spirurida). | proteins from crude extracts of advanced third-stage larvae and adult gnathostoma binucleatum nematode worms showed protein profiles in sds-page analysis similar to echinococcus granulosus, trichinella spiralis, dipylidium caninum, ancylostoma caninum, ascaris lumbricoides and toxocara canis. the immunoblot analysis of the human serum infected or suspected to be infected with g. binucleatum using the total larvae extract recognized the 40, 60, 80 and 115 kda proteins and using the total adult wo ... | 2007 | 17096141 |
| [gnathostoma binucleatum (spirurida: gnathostomatidae) from the freshwater fish in tabasco, mexico]. | human gnathostomiasis is a food-born parasitic disease of relative importance in many countries in southeast asia. it is caused by several species of nematodes of the genus gnathostoma. in mexico is an emerging public health problem since 1970, when first cases were reported. until today, larval morphometric characters that have been proposed to differentiate between the three species of gnathostoma present in this country, are not satisfactory. recently, the presence of advanced third-stage lar ... | 2004 | 17354387 |
| estuarine fish and turtles as intermediate and paratenic hosts of gnathostoma binucleatum in nayarit, mexico. | human gnathostomosis is a severe public health problem in the state of nayarit, mexico. between 1995 and 2005, the registration of human cases numbered 6,328, which makes it one of the largest focal points of the disease in the country. the present study determined the presence of natural hosts of gnathostoma binucleatum larvae at the laguna de agua brava in nayarit, mexico. a total of 5,450 fish and 247 turtles were sampled. muscular tissue was ground and observed against the light using a 100- ... | 2007 | 17924145 |
| identification of estuarine fish dormitator latifrons as an intermediate host and eleotris picta as a paratenic host for gnathostoma binucleatum in sinaloa, mexico. | gnathostomosis is a typical fish-borne zoonotic parasitosis and is currently a serious public health issue in mexico. among several gnathostoma species present in wild animals in mexico, gnathostoma binucleatum is the only proven species responsible for human diseases, and the advanced third stage larvae (al3) of g. binucleatum have been found in over 20 species of fish in this country. in sinaloa state, two fish species, dormitator latifrons and eleotris picta, were heavily contaminated with g. ... | 2008 | 18719943 |
| discovery of an endemic area of gnathostoma turgidum infection among opossums, didelphis virginiana, in mexico. | gnathostomosis, caused by gnathostoma binucleatum, is a serious public health issue in mexico. although 2 other gnathostoma spp., g. turgidum and g. lamothei, have been found in wild animals, their natural life cycle or their relation to human disease remains unclear. while we were conducting an epidemiological survey on gnathostoma spp. in sinaloa state, mexico, we found an endemic area for g. turgidum in common opossums, didelphis virginiana, located in tecualilla, sinaloa. the species identif ... | 2009 | 18989999 |
| gnathostoma binucleatum: pathological and parasitological aspects in experimentally infected dogs. | lesions and antibody kinetics produced by inoculation of gnathostoma binucleatum larvae into dogs are described, as well as the morphology of the recovered parasites. in four out of five infected bitches parasite phases were found in the stomach. only one bitch eliminated eggs and adult parasite phases in feces. in this bitch, the prepatency period lasted 22 weeks and the patency period 14 weeks. necropsy results showed a copiously vascularized 8-cm diameter fibrous nodule lodged in the greater ... | 2011 | 20599438 |
| effect of some physical factors on the viability of third-stage gnathostoma binucleatum larvae. | to diminish the risk of transmission to humans of advanced third-stage larvae (a3l) of gnathostoma binucleatum in fish foods, we evaluated the effects of some physical factors on larval viability. a3l protected within fish meatballs were subjected to freezing, refrigeration, boiling, dry heat, and immersion in lemon juice. by freezing, larvae were killed in 48 h, by refrigeration after 30 days, by boiling in 4 min, and by broiling for 60 min. by lemon juice immersion (ph 2.5), encysted larvae we ... | 2011 | 21549060 |
| ultrastructural changes in the advanced third larval stage of gnathostoma binucleatum almeyda-artigas, 1991 (nematoda: spirurida) following albendazole and albendazole sulfoxide in vitro exposure. | we report here the ultrastructural changes in the third advanced larval stage (al3) of gnathostoma binucleatum almeyda-artigas, 1991, induced by in vitro treatment with albendazole and the metabolite albendazole sulfoxide. during the whole period of the experiment, the controls remained active and vital throughout the 108-h exposure period. the primary site of action of both drugs appears to be the mitochondria and myofibrils of the polymyarian musculature. degenerative changes in the secretory ... | 2011 | 21744019 |
| gnathostomiasis in brazil: an emerging disease with a challenging diagnosis. | this case report describes the second reported case of gnathostomiasis acquired in brazil. the french traveller returned from a sport fishing trip from tocantins where he was repeatedly consuming raw freshwater fish marinated with lemon juice. gnathostoma infection was diagnosed based on clinical symptoms, dietary record and by detection of specific antibodies in the blood. | 2016 | 27799501 |
| cross-reactivity pattern of asian and american human gnathostomiasis in western blot assays using crude antigens prepared from gnathostoma spinigerum and gnathostoma binucleatum third-stage larvae. | gnathostomiasis is a zoonotic parasitosis endemic in many asian and some latin american countries. most human infections are caused by gnathostoma spinigerum in asia and gnathostoma binucleatum in the americas, and recently, imported cases have been increasing among travelers returning from endemic regions. confirmation of the clinical diagnosis relies largely on serologic tests, with a g. spinigerum-antigen-based immunoblot currently being the diagnostic method of choice. however, we repeatedly ... | 2016 | 27325806 |
| presumptive gnathostoma binucleatum-infection in a belgian traveler returning from south america. | | 2017 | 26960751 |
| pathological and parasitological traits in experimentally infected cats with gnathostoma binucleatum (spirurida: gnathostomatidae). | this study aims to describe some of the unknown pathological and parasitological traits of experimental feline gnathostomosis. thirteen female cats were orally inoculated with 30 advanced third-stage gnathostoma binucleatum larvae and were euthanized at various post-infection (p.i.) periods. clinically, the cats presented with nausea, vomiting, abdominal pain and other nonspecific signs. none of the cats shed eggs in their fecal matter. one cat, euthanized at 6 months p.i., developed a fibrous v ... | 2014 | 24877783 |
| identification of immunodominant peptides from gnathostoma binucleatum. | gnathostomiasis is now recognized as a zoonosis with a worldwide distribution. in the americas, it is caused by the third-stage larvae of gnathostoma binucleatum and in asia mainly by g. spinigerum. the availability and preparation of specific antigens are among the main obstacles for developing reliable immunodiagnostic tests. in this study, six immunodominant peptides were identified and characterized from g. binucleatum, somatic antigens (ags: 24, 32, and 40 kda) and excretory-secretory antig ... | 2012 | 22949520 |
| characterization of the humoral immune response against gnathostoma binucleatum in patients clinically diagnosed with gnathostomiasis. | gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked fresh-water fish infected with the advanced third-stage larvae of gnathostoma spp. this disease is endemic to the pacific region of mexico, and one of its etiologic agents has been identified as gnathostoma binucleatum. we characterized the humoral immune response of patients clinically diagnosed with gnathostomiasis by detecting total igm, ige, and igg class and subclasses against a crude extract of ... | 2012 | 22665606 |