| a case of double infection with brugia pahangi buckley and edeson 1956, and dirofilaria immitis leidy 1856, in a malaysian clouded leopard, neofelis nebulosa. | a case of double infection with brugia pahangi and dirofilaria immitis in a clouded leopard, neofelis nebulosa, is presented. a brief review of filarial infections in both man and wild animals, and their medical importance is discussed. | 1986 | 3739206 |
| clouded leopard, neofelis neburosa, new host for dirofilaria immitis. | | 1983 | 6672414 |
| molecular evolution of mitochondrial 12s rna and cytochrome b sequences in the pantherine lineage of felidae. | dna sequence comparisons of two mitochondrial dna genes were used to infer phylogenetic relationships among 17 felidae species, notably 15 in the previously described pantherine lineage. the polymerase chain reaction (pcr) was used to generate sequences of 358 base pairs of the mitochondrial 12s rna gene and 289 base pairs of the cytochrome b protein coding gene. dna sequences were compared within and between 17 felid and five nonfelid carnivore species. evolutionary trees were constructed using ... | 1995 | 7544865 |
| comparative aspects of steroid hormone metabolism and ovarian activity in felids, measured noninvasively in feces. | noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. using the domestic cat (felis catus) as model, the excretory products of injected [14c]estradiol (e2) and [14c]progesterone (p4) were determined. within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered e2 and p4 radioactivity, respectively, was found in feces. e2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme-hydrolyzable conjugate (60%). p4 was excreted ... | 1994 | 7819459 |
| parasites of wild felidae in thailand: a coprological survey. | ninety-two fecal samples were collected from leopards (panthera pardus, n = 54), tigers (p. tigris, n = 19), and leopard cats (felis bengalensis, n = 3) in huai kha wildlife sanctuary, thailand; four samples that may have come from clouded leopards (neofelis nebulosa) or asian golden cats (felis temminicki) were identified as from small to medium cats. twelve samples were identified as from large cats. samples preserved in 10% formalin were examined for parasite eggs, larvae, cysts, and oocysts ... | 1994 | 7933301 |
| regulation of sperm function by protein tyrosine phosphorylation in diverse wild felid species. | protein tyrosine phosphorylation is associated with sperm capacitation and the acrosome reaction in several mammalian species. changes in phosphorylation of a 95-kda protein in human, mouse, and domestic cat spermatozoa are known to be influenced by capacitation and exposure to zona pellucida (zp) proteins. we previously reported diminished phosphorylation of 95- and 160-kda proteins in spermatozoa from teratospermic cats, compared with normospermic domestic cats. to determine if these proteins ... | 1998 | 9876019 |
| feline papillomas and papillomaviruses. | papillomaviruses (pvs) are highly species- and site-specific pathogens of stratified squamous epithelium. although pv infections in the various felidae are rarely reported, we identified productive infections in six cat species. pv-induced proliferative skin or mucous membrane lesions were confirmed by immunohistochemical screening for papillomavirus-specific capsid antigens. seven monoclonal antibodies, each of which reacts with an immunodominant antigenic determinant of the bovine papillomavir ... | 2000 | 10643975 |
| osmotic effects on feline spermatozoa from normospermic versus teratospermic donors. | effects of osmolality stresses on the sperm of normospermic (>60% normal sperm/ejaculate) versus teratospermic (<40% normal sperm) domestic cats and the normospermic leopard cat and the teratospermic clouded leopard were studied. spermatozoa were exposed to various anisotonic solutions in a single step or returned to near isotonic conditions in a single step after exposure to anisotonic solutions. the percentage of sperm motility was measured subjectively, and dual fluorescent stains were used t ... | 2000 | 10788313 |
| a generalized fecal glucocorticoid assay for use in a diverse array of nondomestic mammalian and avian species. | noninvasive fecal glucocorticoid analysis has tremendous potential as a means of assessing stress associated with environmental disturbance in wildlife. however, interspecific variation in excreted glucocorticoid metabolites requires careful selection of the antibody used in their quantification. we compared four antibodies for detecting the major fecal cortisol metabolites in yellow baboons following (3)h cortisol administration, acth challenge, and hplc separation of fecal glucocorticoid metab ... | 2000 | 11121291 |
| phacoemulsification in a clouded leopard (neofelis nebulosa). | phacoemulsification and prophylactic retinopexy were performed on a 3-month-old hand-raised clouded leopard with a unilateral mature cataract. the same procedures were repeated 3 months later when a cataract developed in the fellow eye. examination procedures, anesthetic protocols, and surgical techniques were similar to those utilized in domestic cats. the patient retains highly functional aphakic vision at the present age of 2.5 years. an inherited basis for the cataracts is proposed. although ... | 2001 | 11422992 |
| cytochrome b gene for species identification of the conservation animals. | a partial dna sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to taiwan. the conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan macaques. the control species used included domestic cats, domestic dogs, domestic sheeps, domestic cattle ... | 2001 | 11587860 |
| understanding the basic reproductive biology of wild felids by monitoring of faecal steroids. | the ability to track gonadal activity is essential for understanding the fundamentals of reproduction. faecal steroid metabolite monitoring is a well established tool for evaluating reproductive processes in diverse mammalian species, including felids. domestic cats were used as a model and injection of radiolabelled oestradiol, progesterone, testosterone and cortisol revealed that > 85% of metabolites were excreted in faeces with a time lag of 12-24 h. steroids were extracted by boiling faecal ... | 2001 | 11787193 |
| osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: influence of adding and removing cryoprotectant. | the spermatozoon of felids (cats) survives cryopreservation inconsistently. using ejaculates from three species (domestic cat [normospermic versus teratospermic], the normospermic serval and the teratospermic clouded leopard), this study (1) determined the influence of adding and removing two permeating cryoprotectants (glycerol and dimethylsulfoxide) and (2) assessed the impact of one-step versus multi-step cryoprotectant removal on sperm motility and membrane integrity. spermatozoa were expose ... | 2002 | 12237094 |
| irradiation of diets fed to captive exotic felids: microbial destruction, consumption, and fecal consistency. | two frozen, raw horse meat-based diets fed to captive exotic felids at brookfield zoo were irradiated to determine the extent of microbial destruction and whether radiation treatment would affect consumption and/or fecal consistency in exotic cats. fifteen cats, two african lions (panthera leo), two amur tigers (panthera tigris altaica), one amur leopard (panthera pardus orientalis), two clouded leopards (neofelis nebulosa), two caracals (felis caracal), one bobcat (felis rufus), and five fishin ... | 2001 | 12785680 |
| chromosome evolution in bears: reconstructing phylogenetic relationships by cross-species chromosome painting. | genome-wide homology maps among dog (canis familiaris, cfa, 2n = 78), african lion (panthera leo, ple, 2n = 38), clouded leopard (neofelis nebulosa, nne, 2n = 38) and malayan sun bear (helartos malayanus, hma, 2n = 74) have been established by chromosome painting using a complete set of dog probes. in total, chromosome-specific painting probes from the 38 dog autosomes reveal 69, 69 and 73 conserved segments in african lion, clouded leopard and malayan sun bear, respectively. the chromosomal pai ... | 2004 | 14984102 |
| noninvasive monitoring of adrenocortical activity in carnivores by fecal glucocorticoid analyses. | measurement of glucocorticoid metabolites in feces has become an accepted method for the noninvasive evaluation of adrenocortical activity. the objective of this study was to determine if a simple cortisol enzyme immunoassay (eia) was suitable for monitoring adrenocortical activity in a variety of carnivore species. performance of the cortisol eia was gauged by comparison to a corticosterone radioimmunoassay (ria) that has been used for measuring glucocorticoid metabolites in feces of numerous s ... | 2004 | 15158127 |
| ticks (acari: ixodidae) parasitizing wild carnivores in phu khieo wildlife sanctuary, thailand. | ixodid ticks were collected and identified from 8 wild carnivore species in phu khieo wildlife sanctuary, northeastern thailand. six tick species belonging to 4 genera were recovered and identified from 132 individuals. these included amblyomma testudinarium (n = 36), haemaphysalis asiatica (n = 58), h. hystricis (n = 31), h. semermis (n = 3), rhipicephalus haemaphysaloides (n = 3), and ixodes granulatus (n = 1). leopard cats (prionailurus bengalensis) (n = 19) were infested with 4 tick species, ... | 2004 | 15270119 |
| comparative immobilization of wild felids in thailand. | we immobilized individuals of four free-ranging felid species, leopard cat (prionailurus bengalensis), clouded leopard (neofelis nebulosa), asiatic golden cat (catopuma temminckii), and marbled cat (pardofelis marmorata) with ketamine hydrochloride and xylazine hydrochloride (kh-xh) and with tiletamine hydrochloride and zolazepam hydrochloride (th-zh) between march 1998 and july 2002. mean (+/-sd) dose of kh and xh was 26.51+/-5.71 mg/kg and 1.89+/-0.43 mg/kg, respectively (n=25), and mean dose ... | 2004 | 15465729 |
| phylogenetic studies of pantherine cats (felidae) based on multiple genes, with novel application of nuclear beta-fibrinogen intron 7 to carnivores. | the pantherine lineage of the cat family felidae (order: carnivora) includes five big cats of genus panthera and a great many midsized cats known worldwide. presumably because of their recent and rapid radiation, the evolutionary relationship among pantherines remains ambiguous. we provide an independent assessment of the evolutionary history of pantherine lineage using two complete mitochondrial (mt) genes (nd2 and nd4) and the nuclear beta-fibrinogen intron 7 gene, whose utility in carnivoran ... | 2005 | 15804417 |
| gnrh agonist lupron (leuprolide acetate) pre-treatments prevent ovulation in response to gonadotropin stimulation in the clouded leopard (neofelis nebulosa). | in many species, controlling the ovary prior to induction of ovulation improves the success of ovarian response and artificial insemination (ai). we assessed the impact of suppression of estrus with the gnrh agonist, lupron, on ovarian sensitivity to equine chorionic gonadotropin (ecg) and human chorionic gonadotropin (hcg) in the clouded leopard. seven female clouded leopards were given two injections of lupron (3.75 mg im) 23 d apart, followed 44 d later by ecg and hcg. daily fecal samples wer ... | 2006 | 16513161 |
| molecular evidence for species-level distinctions in clouded leopards. | among the 37 living species of felidae, the clouded leopard (neofelis nebulosa) is generally classified as a monotypic genus basal to the panthera lineage of great cats. this secretive, mid-sized (16-23 kg) carnivore, now severely endangered, is traditionally subdivided into four southeast asian subspecies (figure 1a). we used molecular genetic methods to re-evaluate subspecies partitions and to quantify patterns of population genetic variation among 109 clouded leopards of known geographic orig ... | 2006 | 17141620 |
| clouded leopard phylogeny revisited: support for species recognition and population division between borneo and sumatra. | the clouded leopard (neofelis nebulosa) is one of the least known cat species and depletion of their forested habitats puts it under heavy pressure. recently reclassification of bornean clouded leopards (n. nebulosa diardi) to species level (n.diardi) was suggested based on molecular and morphological evidence. since the genetic results were based solely on three bornean samples we re-evaluated this partition using additional samples of bornean clouded leopards (n = 7) and we were also able to i ... | 2007 | 17535420 |
| the mitochondrial genome structure of the clouded leopard (neofelis nebulosa). | the complete 16 844 bp mitochondrial genome of neofelis nebulosa has been sequenced and compared with the complete mitochondrial genomes of felis catus and the acinonyx jubatus. the base composition of the mitochondrial genome of n. nebulosa is as follows: a, 5343 bp (31.7%); c, 4441 bp (26.4%); g, 2491 bp (14.8%); t, 4569 bp (27.1%). the genome complement and the gene order of this mitochondrial genome was found to be typical of those reported for other mammals. several unusual features of this ... | 2007 | 17546090 |
| detection of toxolasma gondii in captive wild felids. | toxoplasma gondii can infect all species of warm-blooded animals, including humans, and causes serious diseases in immunocompromized hosts. live tachyzoites derived from serial passage in hela culture were used in the sabin-feldman dye test for detection of toxoplasma gondii antibody in serum samples of 21 captive wild felids including one fishing cat (prion nailurus viverrina), one leopard (panthera pardus), two flat-headed cats (prion nailurus planiceps), 6 tigers (panthera tigris), two leopar ... | 2006 | 17547043 |
| challenges in cryopreserving endangered mammal spermatozoa: morphology and the value of acrosomal integrity as markers of cryo-survival. | the science of cryobiology is essential to the effective, practical use of semen for assisted breeding to help manage small populations of rare wildlife species. in this review, we describe challenges associated with cryopreserving gametes from wild fauna. based on more than 25 years of experience across a diversity of mammals, it appears that the primary driving force dictating cryo-survival of a spermatozoon is its initial pre-freeze quality and morphology, especially having a morphologically ... | 2007 | 17644982 |
| characterization of cauxin in the urine of domestic and big cats. | cauxin is an abundant protein in feline urine. we have used proteomics strategies to characterize cauxin from the urine of domestic cats and a number of big cat species. proteins were resolved by gel-based electrophoretic purification and subjected to in-gel digestion with trypsin. the resultant tryptic peptides were mass-measured by matrix-assisted laser desorption ionization time of flight mass spectrometry. peptides were also resolved by liquid chromatography and analyzed by electrospray ioni ... | 2007 | 17924168 |
| the complete mitochondrial genome structure of snow leopard panthera uncia. | the complete mitochondrial genome (mtdna) of snow leopard panthera uncia was obtained by using the polymerase chain reaction (pcr) technique based on the pcr fragments of 30 primers we designed. the entire mtdna sequence was 16 773 base pairs (bp) in length, and the base composition was: a-5,357 bp (31.9%); c-4,444 bp (26.5%); g-2,428 bp (14.5%); t-4,544 bp (27.1%). the structural characteristics [0] of the p. uncia mitochondrial genome were highly similar to these of felis catus, acinonyx jubat ... | 2009 | 18431688 |
| clouded leopard (neofelis diardi) predation on proboscis monkeys (nasalis larvatus) in sabah, malaysia. | in this study, we have reported two direct observations of individuals from a one-male group of proboscis monkeys (nasalis larvatus) being killed by clouded leopards (neofelis diardi) in the riverine forest along the menanggul river, a tributary of the kinabatangan river in sabah, malaysia. one of the two individuals was an infant female and the other was a juvenile female. based on literature reviews and the observations reported here, we suggest that clouded leopard and crocodile might be sign ... | 2008 | 18484152 |
| seroepidemiology of toxoplasma gondii in zoo animals in selected zoos in the midwestern united states. | toxoplasma gondii infections in zoo animals are of interest because many captive animals die of clinical toxoplasmosis and because of the potential risk of exposure of children and elderly to t. gondii oocysts excreted by cats in the zoos. seroprevalence of t. gondii antibodies in wild zoo felids, highly susceptible zoo species, and feral cats from 8 zoos of the midwestern united states was determined by using the modified agglutination test (mat). a titer of 1:25 was considered indicative of t. ... | 2008 | 18605803 |
| selection of river crossing location and sleeping site by proboscis monkeys (nasalis larvatus) in sabah, malaysia. | from may 2005-2006, selections of river crossing locations and sleeping sites used by a one-male group (be-group) of proboscis monkeys (nasalis larvatus) were investigated along the menanggul river, tributary of the kinabatangan river, sabah, malaysia. the frequency of river crossings for focal monkeys in the be-group was significantly higher at locations with narrow branch-to-bank distances. branch-to-bank distances were defined as the distances between the longest tree branches extending over ... | 2008 | 18651612 |
| comparative analysis of paw pad structure in the clouded leopard (neofelis nebulosa) and domestic cat (felis catus). | the clouded leopard (neofelis nebulosa) is a medium-sized highly arboreal cat. this study compares the structure of the digital, metacarpal and metatarsal pads of the manus and pes in n. nebulosa to that of the domestic cat (felis catus). covered by a stratified squamous cornified epithelium, the pads have a supple deposit of subepidermal fat that is partitioned by collagen fibers and extensively anchored to the muscle tendon sheaths. in both animals, a pes metatarsal pad suspensory ligament ori ... | 2009 | 19530159 |
| supermatrix and species tree methods resolve phylogenetic relationships within the big cats, panthera (carnivora: felidae). | the pantherine lineage of cats diverged from the remainder of modern felidae less than 11 million years ago and consists of the five big cats of the genus panthera, the lion, tiger, jaguar, leopard, and snow leopard, as well as the closely related clouded leopard. a significant problem exists with respect to the precise phylogeny of these highly threatened great cats. despite multiple publications on the subject, no two molecular studies have reconstructed panthera with the same topology. these ... | 2010 | 20138224 |
| gordonia neofelifaecis sp. nov., a cholesterol side-chain-cleaving actinomycete isolated from the faeces of neofelis nebulosa. | a cholesterol side-chain-cleaving bacterial strain, ad-6(t), was isolated from fresh faeces of a clouded leopard (neofelis nebulosa) and was studied using a polyphasic taxonomic approach. 16s rrna gene sequence analysis showed that the novel strain formed a distinct subline within the genus gordonia, its closest neighbours being the type strains of gordonia cholesterolivorans, gordonia sihwensis and gordonia hydrophobica, with sequence similarity values of 98.2, 97.8 and 97.6 %, respectively. th ... | 2011 | 20190025 |
| renal lesions of nondomestic felids. | to comprehensively evaluate the occurrence of renal lesions in a variety of nondomestic felids, necropsy cases from 1978 to 2008 were reviewed from a municipal zoo and a large cat sanctuary for those in which the kidneys were examined histologically. seventy exotic felids were identified (25 tigers, 18 lions, 6 cougars, 5 leopards, 3 snow leopards, 3 clouded leopards, 3 canadian lynx, 2 ocelots, 2 bobcats, 2 cheetahs, 1 jaguar), and their histologic renal lesions were evaluated and compared. the ... | 2010 | 20876911 |
| female reproductive cycles of wild female felids. | many felid species are endangered because of destructive human activities. as a result, zoos are being tasked with sustaining genetically healthy populations in case of catastrophic extinctions. unfortunately, with the exception of a few species, most felids do not reproduce well in captivity. the ability to track reproductive activity via hormones is key to developing successful ex situ breeding programs. through the development of noninvasive fecal hormone monitoring techniques, a high degree ... | 2010 | 20888156 |
| chemical characterization of milk oligosaccharides of an african lion (panthera leo) and a clouded leopard (neofelis nebulosa). | the carnivora include the superfamilies canoidea and feloidea. in species of canoidea other than the domestic dog, canis lupus, the milk contains only traces of lactose and much larger concentrations of oligosaccharides. in this study, lactose was found to be the dominant saccharide in the milk or colostrum of two species of feloidea, namely the african lion (panthera leo) and the clouded leopard (neofelis nebulosa). in addition to lactose, the following oligosaccharides were characterized in th ... | 2010 | 21108689 |
| vertebrates used for medicinal purposes by members of the nyishi and galo tribes in arunachal pradesh (north-east india). | arunachal pradesh, the easternmost part of india, is endowed with diverse natural resources and inhabited by a variety of ethnic groups that have developed skills to exploit the biotic resources of the region for food and medicines. information on animals and animal parts as components of folk remedies used by local healers and village headmen of the nyishi and galo tribes in their respective west siang and subansiri districts were obtained through interviews and structured questionnaires. of a ... | 2011 | 21453496 |
| big cat phylogenies, consensus trees, and computational thinking. | abstract phylogenetics seeks to deduce the pattern of relatedness between organisms by using a phylogeny or evolutionary tree. for a given set of organisms or taxa, there may be many evolutionary trees depicting how these organisms evolved from a common ancestor. as a result, consensus trees are a popular approach for summarizing the shared evolutionary relationships in a group of trees. we examine these consensus techniques by studying how the pantherine lineage of cats (clouded leopard, jaguar ... | 2011 | 21563975 |
| polymorphism of cag repeats in androgen receptor of carnivores. | androgen effect is mediated by the androgen receptor (ar). the polymorphism of cag triplet repeat (polycag), in the n-terminal transactivation domain of the ar protein, has been involved either in endocrine or neurological disorders in human. we obtained partial sequence of ar exon 1 in 10 carnivore species. in most carnivore species, polyglutamine length polymorphism presented in all three cag repeat regions of ar, in contrast, only cag-i site polymorphism presented in primate species, and cag- ... | 2011 | 21643744 |
| draft genome sequence of gordonia neofelifaecis nrrl b-59395, a cholesterol-degrading actinomycete. | we report a draft sequence of the genome of gordonia neofelifaecis nrrl b-59395, a cholesterol-degrading actinomycete isolated from fresh feces of a clouded leopard (neofelis nebulosa). as predicted, the reported genome contains several gene clusters for cholesterol degradation. this is the second available genome sequence of the family gordoniaceae. | 2011 | 21742880 |
| mitogenomic analysis of the genus panthera. | the complete sequences of the mitochondrial dna genomes of panthera tigris, panthera pardus, and panthera uncia were determined using the polymerase chain reaction method. the lengths of the complete mitochondrial dna sequences of the three species were 16990, 16964, and 16773 bp, respectively. each of the three mitochondrial dna genomes included 13 protein-coding genes, 22 trna, two rrna, one o(l)r, and one control region. the structures of the genomes were highly similar to those of felis catu ... | 2011 | 22038004 |
| schrodinger's scat: a critical review of the currently available tiger (panthera tigris) and leopard (panthera pardus) specific primers in india, and a novel leopard specific primer. | non-invasive sampling has opened avenues for the genetic study of elusive species, which has contributed significantly to their conservation. where field based identity of non-invasive sample is ambiguous (e.g. carnivore scats), it is essential to establish identity of the species through molecular approaches. a cost effective procedure to ascertain species identity is to use species specific primers (ssp) for pcr amplification and subsequent resolution through agarose gel electrophoresis. howev ... | 2016 | 26860950 |
| identification of gene expression profiles in the actinomycete gordonia neofelifaecis grown with different steroids. | gordonia neofelifaecis nrrl b-59395 was initially isolated from the fresh feces of a clouded leopard based on its ability to degrade cholesterol. the transcriptome profiles of g. neofelifaecis nrrl b-59395 grown with cholesterol, androstenedione (ad), and pyruvic acid were compared by rna-seq. the sterol catabolic genes are highly conserved in g. neofelifaecis, rhodococcus jostii rha1, and mycobacterium tuberculosis. the rna-seq results indicated that the genes involved in the sterol side chain ... | 2014 | 25264805 |
| cutaneous atypical mycobacteriosis in a clouded leopard (neofelis nebulosa). | a 16-yr-old male clouded leopard (neofelis nebulosa) was presented for lethargy and anorexia. a cutaneous abdominal mass extending from the pubis to just caudal to the xiphoid process was present. a biopsy revealed histologic lesions consistent with an atypical mycobacterial infection consisting of diffuse, severe, pyogranulomatous dermatitis and panniculitis, with clear vacuoles and 3-5 microm, intravacuolar, faintly eosinophilic, filamentous bacilli that stained positively with fitefaraco modi ... | 2013 | 24063108 |
| effects of hand-rearing on reproductive success in captive large cats panthera tigris altaica, uncia uncia, acinonyx jubatus and neofelis nebulosa. | species survival plans and european endangered species programmes have been developed for several species of endangered felids in order to build up captive reserve populations and support their conservation in the wild. the siberian tiger (panthera tigris altaica), snow leopard (uncia uncia), cheetah (acinonyx jubatus) and clouded leopard (neofelis nebulosa) are managed in such ex situ conservation programmes. many zoological institutions hand-rear offspring if rearing by the mother fails. hand- ... | 2016 | 27214261 |
| prevalence of valvular regurgitations in clinically healthy captive leopards and cheetahs: a prospective study from the wildlife cardiology (wlc) group (2008-2013). | the purpose of this prospective study was to evaluate transthoracic echocardiograms from clinically healthy large felids for the presence of valvular regurgitations (vr). physiologic vr commonly occur in normal dogs and cats, but the percentage of large felids with vr has not been previously reported. during a 5-yr study period (2008-2013), 28 healthy animals were evaluated under general anesthesia: 16 cheetahs (acinonyx jubatus soemmeringuii) with a mean age of 1.5±0.8 yr (range 0.7-3.5 yr), 5 ... | 2015 | 26352956 |
| sensitivity to exogenous gonadotropins for ovulation induction and laparoscopic artificial insemination in the cheetah and clouded leopard. | ovarian sensitivity to exogenous gonadotropins was assessed in the cheetah (acinonyx jubatus) and clouded leopard (neofelis nebulosa) to help optimize artificial insemination (ai). eighteen female cheetahs were used on 29 occasions and were given i.m. injections of 100, 200, or 400 iu ecg and 100 or 250 iu hcg 80 h later. twenty-three female clouded leopards were treated i.m. on 27 occasions with 25, 50, 75, 100, 200, or 400 iu ecg followed 80 h later with 75, 140, or 280 iu hcg. ovaries were ex ... | 1997 | 9096891 |
| accuracy of mandibular force profiles for bite force estimation and feeding behavior reconstruction in extant and extinct carnivorans. | mandibular force profiles apply the principles of beam theory to identify mandibular biomechanical properties that reflect the bite force and feeding strategies of extant and extinct predators. while this method uses the external dimensions of the mandibular corpus to determine its biomechanical properties, more accurate results could potentially be obtained by quantifying its internal cortical bone distribution. to test this possibility, mandibular force profiles were calculated using both exte ... | 2016 | 27634400 |
| prey depletion as a threat to the world's large carnivores. | large terrestrial carnivores are an ecologically important, charismatic and highly endangered group of species. here, we assess the importance of prey depletion as a driver of large carnivore endangerment globally using lists of prey species for each large carnivore compiled from the literature. we consider spatial variation in prey endangerment, changes in endangerment over time and the causes of prey depletion, finding considerable evidence that loss of prey base is a major and wide-ranging th ... | 2016 | 27853599 |
| genetic relationships among wild felidae in thailand using aflp markers. | the cytogenetics of eight felidae species in thailand were investigated by the colchicines-hypotonic fixation-air drying technique followed by a conventional technique. all species studied have an identical number of 38 diploid chromosomes, indicating a close genetic relationship among species. at a deep study level, the genetic relationships of eight felidae species were accessed by the aflp method. blood samples were collected from sources locating in their original regions for dna extraction. ... | 2007 | 19070075 |
| hip and thigh anatomy of the clouded leopard (neofelis nebulosa) with comparisons to the domestic cat (felis catus). | the clouded leopard (n. nebulosa) is an endangered felid native to southeast asia. it is considered to be the largest and most acrobatic of the arboreal cats. to date, published studies have focused on cranial morphology, particularly osteology and dentition. this study describes the hip and thigh morphology of n. nebulosa and compares it to the domestic cat (f. catus). a number of statistically significant differences were observed between n. nebulosa and f. catus that were primarily associated ... | 2012 | 22287279 |
| personality structure in the domestic cat (felis silvestris catus), scottish wildcat (felis silvestris grampia), clouded leopard (neofelis nebulosa), snow leopard (panthera uncia), and african lion (panthera leo): a comparative study. | although the study of nonhuman personality has increased in the last decade, there are still few studies on felid species, and the majority focus on domestic cats. we assessed the structure of personality and its reliability in five felids-domestic cats, clouded leopards, snow leopards, african lions, and previous data on scottish wildcats-and compared the results. in addition to the benefits of understanding more about this taxon, comparative studies of personality structure have the potential ... | 2014 | 25111629 |
| body mass dynamics in hand reared clouded leopard (neofelis nebulosa) cubs from birth to weaning. | to study the dynamics of body mass changes in hand reared clouded leopards, we analyzed 3,697 weight data points during the first 3 months of life in 49 cubs from 24 zoo-born litters from 2003 through 2012. all cubs were fed the same formula mixture after a similar weaning protocol. the hand rearing process was divided into three periods based on feeding protocols: stage 1: formula only (days 1-28; day 0 = day of birth); stage 2, formula supplemented with protein (e.g., turkey baby food; days 29 ... | 2015 | 25716685 |
| evaluating multispecies landscape connectivity in a threatened tropical mammal community. | habitat corridors are important tools for maintaining connectivity in increasingly fragmented landscapes, but generally they have been considered in single-species approaches. corridors intended to facilitate the movement of multiple species could increase persistence of entire communities, but at the likely cost of being less efficient for any given species than a corridor intended specifically for that species. there have been few tests of the trade-offs between single- and multispecies corrid ... | 2015 | 25065425 |
| accessing local knowledge to identify where species of conservation concern occur in a tropical forest landscape. | conventional biodiversity surveys play an important role in ensuring good conservation friendly management in tropical forest regions but are demanding in terms of expertise, time, and budget. can local people help? here, we illustrate how local knowledge can support low cost conservation surveys. we worked in the malinau watershed, east kalimantan, indonesia, an area currently at risk of extensive forest loss. we selected eight species of regional conservation interest: rafflesia (rafflesia spp ... | 2013 | 23633002 |
| comparative neuronal morphology of the cerebellar cortex in afrotherians, carnivores, cetartiodactyls, and primates. | although the basic morphological characteristics of neurons in the cerebellar cortex have been documented in several species, virtually nothing is known about the quantitative morphological characteristics of these neurons across different taxa. to that end, the present study investigated cerebellar neuronal morphology among eight different, large-brained mammalian species comprising a broad phylogenetic range: afrotherians (african elephant, florida manatee), carnivores (siberian tiger, clouded ... | 2014 | 24795574 |
| clouded leopard predation on a wild juvenile siamang. | i report the first documented case of a wild siamang killed by a clouded leopard. the event, involving a juvenile male (weighing 3.7 kg), occurred at dawn, near a known sleeping tree in a lowland tropical forest in southern sumatra (indonesia). examination of the siamang's body revealed that the first 2 cervical vertebrae were crushed and the face was partially eaten. this attack highlights the potential importance of predation as an evolutionary pressure for hylobatids, and poses some questions ... | 2010 | 21454986 |
| basic haematological values in carnivores--ii. the felidae. | 1. basic haematological values in 34 animals of eight carnivorous species are reported. 2. in four northern lynxs (lynx lynx lynx), two male and two female animals, the mean values are given: erythrocyte counts 8.51 x 10(12)/l, haematocrit 0.392/l, haemoglobin content 148.0 g/l and leukocyte count 7.92 x 10(9)/l. 3. in six male pumas (puma concolor missolensis) the mean values estimated are: erythrocyte count 9.35 x 10(12)/l, haematocrit 0.43/l, haemoglobin content 163.9 g/l and leukocyte count ... | 1987 | 2886279 |
| a molecular phylogeny of the felidae: immunological distance. | the phylogenetic distances between 34 of the 37 extant species of felidae were estimated using albumin immunological distances (aid). albumins from ten cat species were used to prepare antisera in rabbits. a consensus phylogeny was constructed from a matrix of reciprocal aid measurements using four distinct phylogenetic algorithms. a series of one-way measurements using the ten index antisera and those 24 species for which albumins were available (but antisera were not), permitted addition of th ... | 1985 | 28561961 |
| adaptive differences in the body proportions of large felids. | body proportions of eight species of large felids were studied and the relationship between their morphology, habitat structure and habitat utilization was analyzed. both panthera onca and neofelis nebulosa are exclusive forest dwellers (found exclusively in high densly structured habitats). these felids have the relatively shortest anterior limbs and lumbar spine. in addition, p. onca has the relatively shortest posterior limbs of the large felids, while the posterior limbs of n. nebulosa are r ... | 1976 | 973541 |
| how individual links affect network stability in a large-scale, heterogeneous metacommunity. | elucidating how dispersal and landscape connectivity influence metacommunity stability will shed light on natural processes structuring ecosystems and help prioritize conservation actions in an increasingly fragmented world. much of the theoretical and mathematical development of the metacommunity concept has been based on simplified experimental systems or simulated data. we still have limited understanding of how variation in the habitat matrix and species-specific differences in dispersal abi ... | 2016 | 27859156 |
| chemical immobilization of free-ranging and captive sunda clouded leopards (neofelis diardi) with two anesthetic protocols: medetomidine-ketamine and tiletamine-zolazepam. | there is currently no available information regarding the veterinary management of sunda clouded leopards (neofelis diardi), either in captivity or in the wild. in this study, 12 sunda clouded leopards were anesthetized between january 2008 and february 2014 for medical exams, and/or gps-collaring. seven wild-caught individuals were kept in captivity and 5 free-ranging animals were captured by cage traps. two anesthesia combinations were used: medetomidine-ketamine (m-k) or tiletamine-zolazepam ... | 2017 | 28904261 |
| scent marking in sunda clouded leopards (neofelis diardi): novel observations close a key gap in understanding felid communication behaviours. | intraspecific communication is integral to the behavioural ecology of solitary carnivores, but observing and quantifying their communication behaviours in natural environments is difficult. our systematic literature review found that basic information on scent marking is completely lacking for 23% of all felid species, and information on 21% of other felid species comes solely from one study of captive animals. here we present results of the first systematic investigation of the scent marking be ... | 2016 | 27739507 |
| the making of a monster: postnatal ontogenetic changes in craniomandibular shape in the great sabercat smilodon. | derived sabercats had craniomandibular morphologies that in many respects were highly different from those of extant felids, and this has often been interpreted functionally as adaptations for predation at extreme gape angles with hypertrophied upper canines. it is unknown how much of this was a result of intraspecific postnatal ontogeny, since juveniles of sabercats are rare and no quantitative study has been made of craniomandibular ontogeny. postnatal ontogenetic craniomandibular shape change ... | 2012 | 22235326 |
| geographical variation in and evolutionary history of the sunda clouded leopard (neofelis diardi) (mammalia: carnivora: felidae) with the description of a new subspecies from borneo. | recent morphological and molecular studies led to the recognition of two extant species of clouded leopards; neofelis nebulosa from mainland southeast asia and neofelis diardi from the sunda islands of borneo and sumatra, including the batu islands. in addition to these new species-level distinctions, preliminary molecular data suggested a genetic substructure that separates bornean and sumatran clouded leopards, indicating the possibility of two subspecies of n. diardi. this suggestion was base ... | 2011 | 21074625 |
| influence of living status (single vs. paired) and centrifugation with colloids on the sperm morphology and functionality in the clouded leopard (neofelis nebulosa). | the objectives of the present study were to evaluate sperm characteristic of captive clouded leopards in thailand and examine the structural and functional properties of sperm after selection with the single-layer centrifugation (slc) method. twenty-two ejaculates from 11 captive clouded leopards (four housed with access to a female in estrus, and seven housed singly) were collected and assessed for semen traits during 2013 to 2015. twelve fresh ejaculates were chosen from seven males, and each ... | 2016 | 27568458 |
| mitigation of sperm tail abnormalities using demembranation approach in the clouded leopard (neofelis nebulosa). | clouded leopards (neofelis nebulosa) produced high proportion of abnormal spermatozoa (mainly tail defects) that can limit sperm movement and conception. the study aimed to better identify the origin of those defects using a demembranation approach. ejaculates (1-2 ejaculations/male; n = 9) were allocated to simple washing (control; resulting in 11.7% ± 1.9% coiled tails) and processed through colloid centrifugation to reduce the number of sperm with tail defects (treatment, resulting in 5.9% ± ... | 2017 | 27758006 |
| characterization of multiple pathways modulating aggression in the male clouded leopard (neofelis nebulosa). | breeding clouded leopards (neofelis nebulosa) ex situ has been a challenge, primarily due to extreme and often fatal male aggression toward females. this study's aim was to determine the degree to which two possible mechanisms-serotonergic pathways and circulating testosterone levels-affect aggressive behavior. male clouded leopard behavioral and hormonal data were collected during a series of behavior tests administered before and after treatment with either an anxiety-reducing tricyclic antide ... | 2016 | 27588696 |
| histologic and immunohistochemical characterization of pheochromocytomas in 20 clouded leopards ( neofelis nebulosa). | clouded leopards in north american zoological institutions have a high frequency of pheochromocytomas and were identified in 32 of 70 (45%) animals necropsied. archival sections of adrenal gland from 20 adult clouded leopards with unilateral or bilateral pheochromocytomas collected between 1984 and 2011 were examined by light microscopy and immunohistochemistry, and case demographics were reviewed. affected leopards were older than 10 years of age (mean, 16 years; range, 11-19 years), and males ... | 2017 | 27543451 |
| neocortical neuronal morphology in the siberian tiger (panthera tigris altaica) and the clouded leopard (neofelis nebulosa). | despite extensive investigations of the neocortex in the domestic cat, little is known about neuronal morphology in larger felids. to this end, the present study characterized and quantified the somatodendritic morphology of neocortical neurons in prefrontal, motor, and visual cortices of the siberian tiger (panthera tigris altaica) and clouded leopard (neofelis nebulosa). after neurons were stained with a modified golgi technique (n = 194), dendritic branching and spine distributions were analy ... | 2016 | 27098982 |
| comparison of subjective well-being and personality assessments in the clouded leopard (neofelis nebulosa), snow leopard (panthera uncia), and african lion (panthera leo). | the study of subjective well-being in nonhuman animals is growing in the field of psychology, but there are still only a few published studies and the focus is on primates. to consider whether the construct of subjective well-being could be found in another mammal, this study aimed to assess subjective well-being in felids and to examine its association with personality. personality is one of the strongest and most consistent predictors of well-being in humans. this relationship could have impor ... | 2017 | 26983676 |
| swimmer syndrome in a clouded leopard (neofelis nebulosa) cub. | a 32-day-old, hand-reared, captive-born female clouded leopard (neofelis nebulosa) cub presented as being unable to stand, ambulate, or adduct both hind limbs. the cub exhibited hyperextension of both tarsal joints and a flattened thorax, which limited mobility to "swimmer-like" movements. neither congenital defects nor neurologic deficits were observed during the medical examination. radiographic examination showed the thorax was compressed dorsoventrally, but no other skeletal abnormalities we ... | 2014 | 25000703 |
| correspondence regarding 'clouded leopards, the secretive top-carnivore of south-east asian rainforests: their distribution, status and conservation needs in sabah, malaysia'. | | 2007 | 17605794 |
| geographical variation in the clouded leopard, neofelis nebulosa, reveals two species. | the clouded leopard, neofelis nebulosa, is an endangered semiarboreal felid with a wide distribution in tropical forests of southern and southeast asia, including the islands of sumatra and borneo in the indonesian archipelago. in common with many larger animal species, it displays morphological variation within its wide geographical range and is currently regarded as comprising of up to four subspecies. it is widely recognized that taxonomic designation has a major impact on conservation planni ... | 2006 | 17141621 |
| clouded leopards, the secretive top-carnivore of south-east asian rainforests: their distribution, status and conservation needs in sabah, malaysia. | the continued depletion of tropical rainforests and fragmentation of natural habitats has led to significant ecological changes which place most top carnivores under heavy pressure. various methods have been used to determine the status of top carnivore populations in rainforest habitats, most of which are costly in terms of equipment and time. in this study we utilized, for the first time, a rigorous track classification method to estimate population size and density of clouded leopards (neofel ... | 2006 | 17092347 |
| sabertooth characters in the clouded leopard (neofelis nebulosa griffiths 1821). | the clouded leopard (neofelis nebulosa) is an unusual medium-sized felid whose ecology in the wild is poorly known. mainly famous for its large canines, it has often been an overlooked taxon in analyses of felid morphology and systematics or has proven aberrant and difficult to interpret. in this article i report on a number of unusual features in the clouded leopard skull hitherto considered characteristic of sabertooth felids exclusively, and, accordingly, universally believed to be absent in ... | 2006 | 16845677 |
| challenges in cryopreservation of clouded leopard (neofelis nebulosa) spermatozoa. | the clouded leopard (neofelis nebulosa) is an endangered species that is difficult to breed in captivity. species management could benefit from the use of artificial insemination (ai) with frozen-thawed spermatozoa, but there have been no detailed studies of sperm cryosensitivity. the purposes of this study were to: (1) re-characterize seminal characteristics in the clouded leopard 20 years after the first descriptive studies wildt et al., [wildt de, howard jg, hall ll, bush m. reproductive phys ... | 2006 | 16563488 |
| pleural malignant mesothelioma in a captive clouded leopard (neofelis nebulosa nebulosa). | | 1998 | 9698630 |
| intestinal adenocarcinoma with neuroendocrine cells in a clouded leopard (neofelis nebulosa). | adenocarcinoma of the small intestine was found in a 6-year-old clouded leopard. this tumour was characterized by the presence of goblet and neuroendocrine cells. the latter were labelled positively for either serotonin, somatostatin, neurotensin, gastrin or secretin. these neuroendocrine cells were reduced in number in most of the metastatic lesions, including those of the mesentery, diaphragmatic pleura, lungs, liver and heart, and squamous metaplasia was detected in some metastatic sites. | 1996 | 8923240 |
| natural versus chorionic gonadotropin-induced ovarian responses in the clouded leopard (neofelis nebulosa) assessed by fecal steroid analysis. | the clouded leopard (neofelis nebulosa) is an endangered species difficult to propagate in captivity because of sexual incompatibility between paired individuals. fecal estradiol (e2) and progesterone (p4) metabolites were quantified in 14 females to noninvasively monitor seasonal reproductive patterns and compare ovarian responses to natural mating vs. exogenous gonadotropins. increased e2 excretion was associated with behavioral estrus or ecg treatment, whereas elevated p4 metabolites were obs ... | 1995 | 7669862 |
| reproductive physiology of the clouded leopard: i. electroejaculates contain high proportions of pleiomorphic spermatozoa throughout the year. | ejaculates were analyzed from clouded leopards (neofelis nebulosa) subjected to a regimented anesthesia/electroejaculation protocol. group i males (n = 4), maintained individually in an environment with natural fluctuations in photoperiod, were electroejaculated on the same day at monthly intervals (january-december). group ii clouded leopards (n = 8), maintained in random zoo populations throughout the u.s., were evaluated on a single occasion. phase contrast and electron microscopy indicated a ... | 1986 | 3730487 |
| reproductive physiology of the clouded leopard: ii. a circannual analysis of adrenal-pituitary-testicular relationships during electroejaculation or after an adrenocorticotropin hormone challenge. | a circannual analysis was made of serum cortisol, luteinizing hormone (lh), and testosterone concentrations in the male clouded leopard (neofelis nebulosa). group i males (n = 4), maintained in a standardized environment, were bled serially during a regimented anesthesia/electroejaculation episode occurring monthly (beginning in january, ending in december). additional sampling intervals were conducted under anesthesia only (control, n = 8), anesthesia plus a single adrenocorticotropin hormone c ... | 1986 | 3015273 |
| feline viral rhinotracheitis in a colony of clouded leopards. | | 1977 | 203080 |
| [2 formerly unknown types of microfilaria in the blood of the clouded leopard]. | | 1966 | 6011659 |
| down from the treetops: red langur (presbytis rubicunda) terrestrial behavior. | using direct observations and camera traps at eight sites across indonesian borneo we show how red langurs (presbytis rubicunda) are more terrestrial than previously believed, regularly coming to the ground. this unusual behavior has been found at six of the eight sites surveyed. we find that red langurs come to the ground more frequently in disturbed forests, specifically ones which have been impacted by logging, fire, and hunting, though more data are needed to confirm this as a direct correla ... | 2018 | 30027488 |
| suspected sunda clouded leopard (neofelis diardi) predation attempts on two reintroduced bornean orangutans (pongo pygmaeus wurmbii) in bukit batikap protection forest, central kalimantan, indonesia. | in february 2017 and august 2018, respectively, two bornean orangutans (pongo pygmaeus wurmbii) reintroduced into the bukit batikap protection forest in central kalimantan were found in weakened physical condition and with deep puncture wounds. the first individual was a sub-adult male, and the second an adult female whose 6- to 8-week-old infant was missing. both individuals were rescued and transported back to the field base camp for treatment. experienced veterinarians treating the injuries r ... | 2020 | 32623603 |
| practical application of laparoscopic oviductal artificial insemination for the propagation of domestic cats and wild felids. | ai was first reported in cats almost 50 years ago but, unlike ai in other domesticated animals (e.g. dogs, cattle, horses), has not been widely used for routine propagation by veterinarians or breeders. anatomical and physiological challenges with cats have hindered the efficiency of ai using standardised transcervical approaches applied to other species. development of laparoscopic oviductal ai (lo-ai) has helped overcome some of these barriers and, during the past 7 years, produced high pregna ... | 2018 | 32188540 |
| identification of feline kiss1 and distribution of immunoreactive kisspeptin in the hypothalamus of the domestic cat. | in recent years, the kiss1 gene has been reported in a number of vertebrate species, and a substantial dataset has been acquired to demonstrate the critical role of kisspeptins in the reproductive system; yet limited information is available for carnivores. in the present study, we identified and characterized feline kiss1 by isolating and cloning its full-length cdna in the domestic cat hypothalamus and caracal testis, using the method of rapid amplification of cdna ends. additionally, we isola ... | 2019 | 31142694 |
| analysis of metabolic flux in felid spermatozoa using metabolomics and 13c-based fluxomics†. | spermatozoa from three feline species-the domestic cat (felis catus), the cheetah (acinonyx jubatus), and the clouded leopard (neofelis nebulosa)-were analyzed using metabolomic profiling and 13c-based fluxomics to address questions raised regarding their energy metabolism. metabolic profiles and utilization of 13c-labeled energy substrates were detected and quantified using gas chromatography-mass spectrometry (gc-ms). spermatozoa were collected by electroejaculation and incubated in media supp ... | 2019 | 30715249 |
| serological evidence of influenza virus infection in captive wild felids, thailand. | influenza virus is known to affect wild felids. to explore the prevalence of influenza viruses in these animal species, 196 archival sera from 5 felid species including panthera tigris (n=147), prionailurus viverrinus (n=35), panthera leo (n=5), pardofelis temminckii (n=8) and neofelis nebulosa (n=1) collected between 2011 and 2015 in 10 provinces of thailand were determined for the presence of antibody to avian and human influenza viruses. blocking enzyme-linked immunosorbent (elisa) assay and ... | 2019 | 31341136 |
| population dynamics of threatened felids in response to forest cover change in sumatra. | habitat loss caused by deforestation is a global driver of predator population declines. however, few studies have focussed on these effects for mesopredator populations, particularly the cryptic and elusive species inhabiting tropical rainforests. we conducted camera trapping from 2009-11 and 2014-16, and used occupancy modelling to understand trends of sumatran mesopredator occupancy in response to forest loss and in the absence of threats from poaching. by comparing the two survey periods we ... | 2020 | 32785217 |
| first evaluations and cryopreservation of semen samples from sunda clouded leopards (neofelis diardi). | a better understanding of semen characteristics and resilience to freezing temperatures is necessary before developing assisted reproductive techniques and systematic biobanking for the sunda clouded leopard. the objective of this study was to evaluate for the first time the semen and sperm quality (in fresh and frozen samples) of two captive sunda clouded leopards in malaysia. a total of 17 examinations of the reproductive tract (using ultrasonography) and electro-ejaculations were performed on ... | 2020 | 32580372 |
| clouded leopard (neofelis nebulosa) morbidity and mortality in captive-bred populations: a comprehensive retrospective study of medical data from 271 individuals in european, asian, and australian zoos. | the clouded leopard (neofelis nebulosa) is classified as vulnerable on the international union for the conservation of nature red list of threatened species. however, diseases affecting this species across zoo populations are not well documented. the primary objective of this retrospective study was to identify common and significant causes of morbidity and mortality in captive-bred clouded leopards from european, asian, and australian institutions. medical records from 44 zoological parks that ... | 2020 | 32212558 |
| assessing the spatiotemporal interactions of mesopredators in sumatra's tropical rainforest. | co-occurrence between mesopredators can be achieved by differentiation of prey, temporal activity, and spatial habitat use. the study of mesopredator interactions is a growing area of research in tropical forests and shedding new light on inter-guild competition between threatened vertebrate species that were previously little understood. here, we investigate sympatry between the sunda clouded leopard (neofelis diardi) and asiatic golden cat (pardofelis temminckii) living in the sumatran rainfor ... | 2018 | 30231043 |
| simulating impacts of rapid forest loss on population size, connectivity and genetic diversity of sunda clouded leopards (neofelis diardi) in borneo. | habitat loss is the greatest threat to biodiversity in borneo, and to anticipate and combat its effects it is important to predict the pattern of loss and its consequences. borneo is a region of extremely high biodiversity from which forest is being lost faster than in any other. the little-known sunda clouded leopard (neofelis diardi) is the top predator in borneo and is likely to depend critically on habitat connectivity that is currently being rapidly lost to deforestation. we modeled the eff ... | 2018 | 30208031 |
| directional selection in the evolution of elongated upper canines in clouded leopards and sabre-toothed cats. | extremely developed or specialized traits such as the elongated upper canines of extinct sabre-toothed cats are often not analogous to those of any extant species, which limits our understanding of their evolutionary cause. however, an extant species may have undergone directional selection for a similar extreme phenotype. among living felids, the clouded leopard, neofelis nebulosa, has exceptionally long upper canines for its body size. we hypothesized that directional selection generated the e ... | 2018 | 29904973 |
| identifying important conservation areas for the clouded leopard neofelis nebulosa in a mountainous landscape: inference from spatial modeling techniques. | the survival of large carnivores is increasingly precarious due to extensive human development that causes the habitat loss and fragmentation. habitat selection is influenced by anthropogenic as well as environmental factors, and understanding these relationships is important for conservation management. we assessed the environmental and anthropogenic variables that influence site use of clouded leopard neofelis nebulosa in bhutan, estimated their population density, and used the results to pred ... | 2018 | 29721297 |
| successful laparoscopic oviductal artificial insemination in the clouded leopard (neofelis nebulosa) in thailand. | captive breeding of clouded leopards (neofelis nebulosa) is challenging because of mating incompatibility, high incidence of teratospermia in males, and inconsistent ovulation patterns in females. assisted reproductive techniques, therefore, are necessary to overcome these issues and maintain the genetic diversity in the captive population. the objective was to use laparoscopic oviductal artificial insemination (ai) to breed genetically valuable females (n = 4; aged 4.5-5 yr) that were unsuccess ... | 2017 | 28920796 |