Publications

TitleAbstractYear
Filter
PMID
Filter
suppression of phytohemagglutinin response by fungi from a "leukemia" house.fungal isolates from the house of a husband and wife who both developed acute myelomonocytic leukemia were assayed for effects on the in vivo response to phytohemagglutinin in guinea pigs. skin responses to intradermal phytohemagglutinin were measured following injections of sterile fungal extracts. isolates of penicillium canescens, curvularia, fusarium sambucinum, fusarium equiseti and trichoderma koningii from the leukemia-associated house depressed the responses to phytohemagglutinin, but no ...1979291385
the post-harvest fruit rots of tomato (lycopersicum esculentum) in nigeria.a survey of the post-harvest fruit rot diseases of tomato was conducted in five states of nigeria. during severe infections, the diseases could cause 25% loss at harvest and 34% loss of the remaining product in transit, storage and market stalls; thus giving an overall loss of about 50% of the product. two types of rots, soft and dry were recognised. the soft rot was found to account for about 85% and the dry rot about 15% of the overall loss. erwinia carotovora, rhizopus oryzae, r. stolonifer, ...1979471028
fusarium equiseti associated mycotoxins as possible cause of degnala disease.in an attempt to find out the etiology of degnala disease, occurring among buffaloes and zebu cattle in certain rice growing areas of india, preliminary field and laboratory investigations were conducted. on the basis of information so obtained, certain hypotheses (possible causes) were formulated and each of them was examined by instituting laboratory and experimental studies. these investigations suggested the possibility of the involvement of mycotoxins.1977613929
changes in ascorbic acid and carbohydrate contents in tomato fruits infected with pathogens.tomato fruits var romer vfn, inoculated with fusarium equiseti, f. chlamydosporium, geotrichum candidum, acremonium recifei, aspergilus flavus and a. niger showed decline in ascorbic acid content with days of incubation when compared with control (uninoculated) fruits. the total soluble sugars of the inoculated fruits also showed a reduction trend as the storage period was prolonged, but slight increase was observed on the 6th day after inoculation.19921438080
isolation and characterization of zearalenone sulfate produced by fusarium spp.a water-soluble compound related to zearalenone was isolated from a culture of fusarium graminearum 30 grown in rice. the structure of the novel metabolite was determined to be zearalenone-4-sulfate on the basis of fast-atom-bombardment mass spectrometry, proton nuclear magnetic resonance, uv spectroscopy, and by chemical and enzymatic reactions. strains representing fusarium equiseti, fusarium sambucinum, and fusarium roseum produced the sulfate conjugate as well. in the rat uterus enlargement ...19911827972
decreased immune response and increased incidence of tibial dyschondroplasia caused by fusaria grown on sterile corn.corn, feed, and litter samples reported to be associated with feed refusal, diarrhea, leg weakness, and mortality were evaluated for the presence of toxic substances. intubated residues of ethyl acetate extracts of these samples did not cause gross lesions, diarrhea, or mortality in young new hampshire x single comb white leghorn crossbred chicks. fusarium moniliforme was the predominant fungal species found in unpelleted feed and corn samples. young broiler chicks, fed diets supplemented with 2 ...19912027835
natural occurrence of the mycotoxin fusarochromanone, a metabolite of fusarium equiseti, in cereal feed associated with tibial dyschondroplasia.the mycotoxin fusarochromanone, a metabolite of fusarium fungi, is able to induce tibial dyschondroplasia (td) in chickens under experimental conditions. on the basis of health surveillance data on td, two broiler farms with td prevalence rates of up to 56% were identified. in the corresponding pelleted feed samples, fusarochromanone was detected in all 12 samples analyzed by column purification and tlc, with concentrations 4 to 59 micrograms/kg. no fusarium fungi were available from the feed be ...19892619307
mycological survey of korean cereals and production of mycotoxins by fusarium isolates.the fungal species isolated from korean cereals (barley, polished barley, wheat, rye, and malt) were alternaria spp., aspergillus spp., chaetomium spp., drechslera spp., epicoccum sp., fusarium spp., and penicillium spp., etc. the number of fusarium strains isolated was 36, and their ability to produce fusarium mycotoxins on rice was tested. nivalenol (niv) was produced by fusarium graminearum (7 of 9 isolates), fusarium oxysporum (3 of 10 isolates), and fusarium spp. (7 of 15 isolates). of 15 i ...19862947538
acid phosphatase activity of chondroclasts from fusarium-induced tibial dyschondroplastic cartilage.tibial dyschondroplasia was induced in broiler chickens by oral administration of fusarochromanone, the toxic component of fusarium equiseti. in two experiments, the activity of acid phosphatase in chondroclasts was assessed histochemically. chicks were examined at 7, 14, 21, and 28 days of treatment in expt. 1 and at 2, 4, and 6 days of treatment in expt. 2. the staining for acid phosphatase was consistently lower in fusarochromanone-treated chicks after 2 days of treatment than in age-matched ...19883401171
in vitro characteristics on human lymphocyte functions of a new immunomodulatory agent, a cyclic peptide, cyclomunine.cyclomunine, a cyclic peptide extracted from fusarium equisiti, inhibits responses of human lymphocytes to mitogens, soluble antigens and allogeneic cells and the proliferation of lymphoblastoid cell lines. cyclomunine has little effect on small lymphocytes but acts rather on lymphoblasts. it has no effect on fibroblasts and myeloid cells. cyclomunine partially inhibits the generation of suppressor cells induced by con a and the generation of cytotoxic t cells in a mixed lymphocyte culture and t ...19806451339
mycotoxins in animal feeds and toxic fungi in queensland 1971-80.during the years 1971 to 1980, 355 samples of animal feeds submitted to this laboratory in connection with suspected mycotoxicoses were analysed for aflatoxins. most major queensland agricultural crops were represented. aflatoxins were detected in 67 samples, 45 of which were peanut meals and by-products (55% of all peanut materials analysed). of 200 aspergillus flavus isolates cultured on maize meal, aflatoxins were produced by 49%. ochratoxin a was detected in one sample of another 25 feeds an ...19816803756
bacterial expression of catalytically active fragments of the multifunctional enzyme enniatin synthetase.enniatin synthetase catalyzes the biosynthesis of n-methylated cyclohexadepsipeptides. the 347 kda enzyme is encoded by the esyn1 gene of fusarium scirpi and contains two domains (ea and eb) homologous to each other and to regions of other microbial peptide synthetases. parts of the esyn1 gene were subcloned in frame to a small lacz gene portion of escherichia coli expression vectors. overproduced recombinant proteins showed a high tendency towards inclusion body formation and could be only part ...19947932733
in vitro sensitivity of medically significant fusarium species to various antimycotics.sixteen isolates belonging to fusarium chlamydosporum (n = 4), fusarium equiseti (n = 1), fusarium moniliforme (n = 2), fusarium oxysporum (n = 3), fusarium proliferatum (n = 1), and fusarium solani (n = 5) were tested against amphotericin b, 5-fluorocytosine, fluconazole, itraconazole, ketoconazole, jai-amphotericin b (water-soluble compound), hamycin and amphotericin b combined with 5-fluorocytosine, using antibiotic medium m3, high-resolution broth (ph 7.1), sabouraud's dextrose, and yeast-ni ...19948082411
effects of equisetin on rat liver mitochondria: evidence for inhibition of substrate anion carriers of the inner membrane.the effect of equisetin, an antibiotic produced by fusarium equiseti, has been studied on mitochondrial functions (respiration, atpase, ion transport). equisetin inhibits the dnp-stimulated atpase activity of rat liver mitochondria and mitoplasts in a concentration-dependent manner; 50% inhibition is caused by about 8 nmol equisetin/mg protein. the antibiotic is without effect either on the atpase activity of submitochondrial particles or on the purified f1-atpase. it inhibits both the adp- or d ...19938132493
molecular characterization of the enniatin synthetase gene encoding a multifunctional enzyme catalysing n-methyldepsipeptide formation in fusarium scirpi.the gene encoding the multifunctional enzyme enniatin synthetase from fusarium scirpi (esyn1) was isolated and characterized by transcriptional mapping and expression studies in escherichia coli. this is the first example of a gene encoding an n-methyl peptide synthetase. the nucleotide sequence revealed an open reading frame of 9393 bp encoding a protein of 3131 amino acids (m(r) 346,900). two domains designated ea and eb within the protein were identified which share similarity to each other a ...19938483420
an exoantigen test for the rapid identification of medically significant fusarium species.the accurate identification of fusarium species can take 2-3 weeks. preliminary exoantigen studies indicate that a mature culture suspected of being a fusarium species may be immunologically identified 48 h after receipt. exoantigen extracts of 10-day-old slant cultures of fusarium chlamydosporum, fusarium moniliforme (= fusarium verticilloides), fusarium oxysporum, fusarium proliferatum and fusarium solani and partially purified reference homologous and heterologous shake culture extracts (6-we ...19958544080
influence of fungi associated with bananas on nutritional content during storage.botryodiplodia theobromae, rhizopus oryzae, aspergillus niger, a. flavus and fusarium equiseti were found to be associated with the ripening of bananas and also caused rot during storage. bananas stored in baskets with ash fire wood ripened 2-3 days earlier than bananas stored in fibre sacks and under constant light. the infected bananas showed a decrease in the quantity of total soluble sugars, protein, lipid, crude fibre, ash, ascorbic acid and mineral elements when compared with the control f ...19968711953
in-vitro antifungal susceptibility of clinical and environmental fusarium spp. strains.the mics of amphotericin b, miconazole, ketoconazole, flucytosine, itraconazole and fluconazole for 19 isolates of fusarium oxysporum, 16 fusarium solani, seven fusarium verticilliodes, four fusarium proliferatum, four fusarium dimerum, three fusarium equiseti, and one each of the following species: fusarium graminearum, fusarium chlamydosporum, fusarium semitectum, fusarium avenaceum and fusarium subglutinans were determined by a broth microdilution method. thirty-eight of these isolates were o ...19979069536
fusarium species from nepalese rice and production of mycotoxins and gibberellic acid by selected species.infection of cereal grains with fusarium species can cause contamination with mycotoxins that affect human and animal health. to determine the potential for mycotoxin contamination, we isolated fusarium species from samples of rice seeds that were collected in 1997 on farms in the foothills of the nepal himalaya. the predominant fusarium species in surface-disinfested seeds with husks were species of the gibberella fujikuroi complex, including g. fujikuroi mating population a (anamorph, fusarium ...200010698766
mutational analysis of the n-methyltransferase domain of the multifunctional enzyme enniatin synthetase.n-methylcyclopeptides like cyclosporins and enniatins are synthesized by multifunctional enzymes representing hybrid systems of peptide synthetases and s-adenosyl-l-methionine (adomet)-dependent n-methyltransferases. the latter constitute a new family of n-methyltransferases sharing high homology within procaryotes and eucaryotes. here we describe the mutational analysis of the n-methyltransferase domain of enniatin synthetase from fusarium scirpi to gain insight into the assembly of the adomet- ...200010887181
nematode-antagonistic trichothecenes from fusarium equiseti.a strain of the fungus fusarium equiseti isolated from soybean cyst nematode secretes nematode-antagonistic compounds. bioassay-guided fractionation of an extract of the culture broth was undertaken to identify the compounds. fractions were assayed for activity against a root-knot nematode (meloidogyne incognita), a plant pathogen that attacks the roots of numerous plant species. two trichothecene compounds were isolated that inhibited egg hatch and immobilized second-stage juveniles of this nem ...200111471940
production of trichothecenes and other secondary metabolites by fusarium culmorum and fusarium equiseti on common laboratory media and a soil organic matter agar: an ecological interpretation.fusarium culmorum and f. equiseti were characterized with regard to production of trichothecenes and other secondary metabolites. results following growth on laboratory media are interpreted with the aim of increasing the understanding of fungal metabolism in the field environment. while trichothecene production was detected for 94 of 102 f. culmorum isolates, only 8 of 57 f. equiseti isolates were positive. profiles of secondary metabolites were compared by following growth on yeast extract suc ...200212475276
an oligonucleotide microarray for the identification and differentiation of trichothecene producing and non-producing fusarium species occurring on cereal grain.cereal grain may be infected with a number of fusarium species some of which are producers of highly toxic compounds such as the trichothecenes. correct identification of these species is essential for risk assessment of cereal grain for human or animal consumption. most of the available methods for identification are either time consuming or aimed at only one or a few target species. microarray technology offers parallel analysis of a high number of dna targets. in this study 57 capture oligonu ...200515823394
antibiotic produced by fusarium equiseti nrrl 5537.fusarium equiseti nrrl 5537 grown on an autoclaved white corn grit medium for 3 to 4 weeks at room temperature produced a substance in excess of 5 g/kg of substrate that inhibited some gram-positive bacteria including mycobacteria. most bacillus subtilis, mycobacterium phlei, and staphylococcus aureus strains were inhibited when 1 mug of the antibiotic per ml was incorporated into the culture medium. except for neisseria perflava, gram-negative bacteria, yeasts, and molds were not inhibited by 1 ...197415825417
species-specific pcr-based assays for the detection of fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis.species-specific pcr was used for the identification of nine fusarium species in pure mycelial culture. a pcr-based method was compared with the whole seed agar plate method and trichothecene analysis for three toxin-producing fusarium species using 85 grain samples of wheat, barley, oat, corn and rye. a simple sds-based dna extraction system followed by potassium acetate precipitation resulted in consistent pcr amplification of dna fragments from cultures and grain samples. the species-specific ...200516099312
pcr detection assays for the trichothecene-producing species fusarium graminearum, fusarium culmorum, fusarium poae, fusarium equiseti and fusarium sporotrichioides.contamination of small-grain cereals with the fungal species fusarium graminearum, f. culmorum, f. poae, f. sporotrichioides and f. equiseti is an important source of trichothecenes, zearalenone and other mycotoxins which cause serious diseases in human and animals. additionally, these species contribute to fusarium head blight, a disease which produces important losses in cereal yield. early detection and control of these fusarium species is crucial to prevent toxins entering the food chain and ...200516104354
a comparative study of the metal ion uptake and antioxidant enzyme activities of fusarium equiseti and fusarium acuminatum as a function of external magnesium concentration.the increase of mg2+, from 1.3 to 3 microm, in growth medium of f. equiseti and f. acuminatum increased intracellular magnesium levels from 0.83 and 0.81 microm to 1.75 and 1.42 microm on the 12th day, respectively. intracellular magnesium levels also elevated depending upon the number of incubation days. the maximum manganese levels of f. equiseti and f. acuminatum obtained in 1.6 microm mg2+ culture medium were 0.67 and 1.23 microm, while maximum iron levels were determined to be 1.3 microm mg ...200516109634
the mycoestrogen zearalenone induces cyp3a through activation of the pregnane x receptor.zearalenone is a mycoestrogen that is produced in the fungi fusarium graminearum, fusarium culmorum, fusarium equiseti, and fusarium crookwellense. these fungi commonly exist in agricultural products. human pregnane x receptor (hpxr) is a ligand-activated transcription factor that regulates the expression of numerous hepatic drug-metabolizing enzymes, including several clinically important cytochrome p450s. in this report, we show that zearalenone is an efficacious ligand for hpxr. we also descr ...200616547076
functional characterization of the recombinant n-methyltransferase domain from the multienzyme enniatin synthetase.a 51 kda fusion protein incorporating the n-methyltransferase domain of the multienzyme enniatin synthetase from fusarium scirpi was expressed in saccharomyces cerevisiae. the protein was purified and found to bind s-adenosyl methionine (adomet) as demonstrated by cross-linking experiments with (14)c-methyl-adomet under uv irradiation. cofactor binding at equilibrium conditions was followed by saturation transfer difference (std) nmr spectroscopy, and the native conformation of the methyltransfe ...200717471480
[experimental study of fast diagnosis of mycotic keratitis using molecular biology technical].to establish a method for fast diagnosis of mycotic keratitis using multi-pcr system.200717605910
toxigenic fungi and mycotoxins in mature corn silage.to investigate the exposure of livestock and farm workers to mycotoxins during the last months of silage use, the mycoflora and the mycotoxins in a mature silage (11-months-old) were studied. a multimycotoxin method was developed to evaluate the toxigenic in vitro ability of fungal strains. the screening of potentially toxigenic fungi isolated from the mature silage showed that six fusaria (fusarium culmorum, fusarium equiseti, fusarium graminearum, fusarium oxysporum, fusarium solani and fusari ...200717655998
inhibitory effects of fusarochromanone on melanoma growth.fusarochromanone is a toxic metabolite produced by fusarium equiseti, a fungus present in decaying cereal plants in northern latitudes; it has been detected in various food grains. fusarochromanone has been shown to have both stimulatory and inhibitory effects on various mammalian cells, depending on the concentration used. whether these cytotoxic effects can be used in the clinical treatment of tumors remains to be established. here, we evaluated the effects of fusarochromanone on the growth of ...200717667595
degradation of hop bitter acids by fungi.nine fungal strains related to: trametes versicolor, nigrospora oryzae, inonotus radiatus, crumenulopsis sororia, coryneum betulinum, cryptosporiopsis radicicola, fusarium equiseti, rhodotorula glutinis and candida parapsilosis were tested for their ability to degrade humulones and lupulones. the best results were obtained for t. versicolor culture, in which humulones and lupulones were fully degraded after 4days of incubation in the dark or after 36h in the light. the experiments were performed ...200817845845
some fungi, zearalenone and other mycotoxins in chicken rations, stock feedstuffs, lucerne and pasture grasses in the communal farming area of rhenosterkop in south africa.mycotoxins may be present in feeds without any visible signs of mould contamination. there is a need for rapid and accurate measurement of mycotoxins for purposes of continual monitoring and identification of high risk commodities. samples from commercial chicken feed (maize kernels), cattle feed (lucerne, grass and hay) and milk were analysed for the presence of certain mycotoxins and cultured for fungi. results of fungal profiles showed that most samples were contaminated by moulds belonging t ...200717941598
fungal root endophytes from natural vegetation in mediterranean environments with special reference to fusarium spp.surveys (in 2002 and 2003) were performed for fungal endophytes in roots of 24 plant species growing at 12 sites (coastal and inland soils, both sandy soils and salt marshes) under either water or salt stress in the alicante province (southeast spain). all plant species examined were colonized by endophytic fungi. a total of 1830 fungal isolates were obtained and identified by morphological and molecular [internal transcribed spacer (its) and translation elongation factor-1alpha gene region (tef ...200818248439
colonization of barley roots by endophytic fungi and their reduction of take-all caused by gaeumannomyces graminis var. tritici.fungal root endophytes obtained from natural vegetation were tested for antifungal activity in dual culture tests against the root pathogen gaeumannomyces graminis var. tritici. fifteen isolates, including acremonium blochii, acremonium furcatum, aspergillus fumigatus, cylindrocarpon sp., cylindrocarpon destructans, dactylaria sp., fusarium equiseti, phoma herbarum, phoma leveillei, and a sterile mycelium, selected based on the dual culture test, were inoculated on barley roots in growth tubes u ...200818772922
real-time pcr quantification and live-cell imaging of endophytic colonization of barley (hordeum vulgare) roots by fusarium equiseti and pochonia chlamydosporia.*new tools were developed for the study of the endophytic development of the fungal species fusarium equiseti and pochonia chlamydosporia in barley (hordeum vulgare) roots. these were applied to monitor the host colonization patterns of these potential candidates for biocontrol of root pathogens. * molecular beacons specific for either f. equiseti or p. chlamydosporia were designed and used in real-time polymerase chain reaction (pcr) quantification of fungal populations in roots. genetic transf ...200919170898
in-vitro assays of meloidogyne incognita and heterodera glycines for detection of nematode-antagonistic fungal compounds.in-vitro methods were developed to test fungi for production of metabolites affecting nematode egg hatch and mobility of second-stage juveniles. separate assays were developed for two nematodes: root-knot nematode (meloidogyne incognita) and soybean cyst nematode (heterodera glycines). for egg hatch to be successfully assayed, eggs must first be surface-disinfested to avoid the confounding effects of incidental microbial growth facilitated by the fungal culture medium. sodium hypochlorite was mo ...199919270887
use of mass spectrometry to identify clinical fusarium isolates.fusarium spp. have recently emerged as significant human pathogens. identification of these species is important, both for epidemiological purposes and for patient management, but conventional identification based on morphological traits is hindered by major phenotypic polymorphism. in this study, 62 strains, or isolates, belonging to nine fusarium species were subjected to both molecular identification tef1 gene sequencing and matrix-assisted laser desorption ionization-time-of-flight (maldi-to ...200919456834
assessing fungal root colonization for plant improvement.fungal endophytes display a broad range of symbiotic interactions with their host plants. current studies on their biology, diversity and benefits are unravelling their high relevance on plant adaptation to environmental stresses. implementation of such properties may open new perspectives in agriculture and forestry. we aim to exploit the endophytic capacities of the fungal species fusarium equiseti, a naturally occurring root endophyte which has shown antagonism to plant pathogens, and pochoni ...200919816102
pathogenicity of fusarium isolates to striga hermonthica in burkina faso.striga hermonthica (del.) benth. is an important constraint to cereal crop production in burkina faso, of which sorghum (sorghum bicolor l. moench) is the most important component. native fusarium species to use as bio-control agents to s. hermonthica has been investigated. fifty one fusarium isolates obtained from diseased plants of s. hermonthica were evaluated for their pathogenicity against striga under controlled environmental conditions. of 51 fusarium isolates, 14 were pathogenic to s. he ...201020464941
effect of biofumigation with manure amendments and repeated biosolarization on fusarium densities in pepper crops.in the region of murcia (southeast spain), sweet pepper has been grown as a monoculture in greenhouses for many years. until 2005, when it was banned, soils were disinfested with methyl bromide (mb) to control pathogens and to prevent soil fatigue effects. the genus fusarium plays an important role in the microbiological component associated with yield decline in pepper monocultures. in the present study, soils were treated with manure amendments, alone (biofumigation, b) or in combination with ...201120820866
solar disinfection of fungal spores in water aided by low concentrations of hydrogen peroxide.our previous contribution showed that fusarium solani spores are inactivated by low amounts of hydrogen peroxide (lower than 50 mg l(-1)) together with solar irradiation in bottles. the purpose of the current study was to evaluate the effectiveness of solar h(2)o(2)/uv-vis in distilled water and simulated municipal wastewater treatment plant effluent (se) contaminated with chlamydospores of fusarium equiseti in a 60 l solar cpc photo-reactor under solar irradiation. this study showed that f. equ ...201120859602
plant growth-promoting fungus, trichoderma koningi suppresses isoflavonoid phytoalexin vestitol production for colonization on/in the roots of lotus japonicus.the relationship between the colonization of lotus japonicus by plant growth-promoting fungi (pgpf) and biosynthesis of the isoflavonoid phytoalexin vestitol, a major defensive response of leguminous plants, was analyzed. when pgpf including trichoderma koningi, fusarium equiseti, and penicillium simplicissimum were inoculated onto l. japonicus roots, only t. koningi colonized the roots long-term and increased plant dry weight (126%). microscopic observations of transverse sections of roots colo ...201121502738
prevalence of deg nala disease in eastern india and its reproduction in buffaloes by feeding fusarium oxysporum infested rice straw.to undertake a study on prevalence of deg nala disease in eastern states of india and to reproduce the disease in buffaloes by the fusarium spp., isolated from the affected region.201121771416
extracellular xylanase production by fusarium species in solid state fermentation.fusarium sp. has been shown to be a promising organism for enhanced production of xylanases. in the present study, xylanase production by 21 fusarium sp. isolates (8 fusarium culmorum, 4 fusarium solani, 6 fusarium verticillioides and 3 fusarium equiseti) was evaluated under solid state fermentation (ssf). the fungal isolate fusarium solani syrn7 was the best xylanase producer among the tested isolates. the effects of some agriculture wastes (like wheat straw, wheat bran, beet pulp and cotton se ...201122184927
production of zearalenone in vitro and in corn grains stored under modified atmospheres.the production of zearalenone by an isolate of fusarium equiseti was studied in chemically defined medium and in corn grains stored under modified atmospheres. an increase in the concentrations of sucrose or xylose in czapek's medium resulted in increased toxin production, while no toxin was produced when lactose was present in the medium. methionine (10(-2) and 10(-3) m) and cystine (10(-3) m) added to czapek's medium inhibited zearalenone production. when amino acids or nitrogen salts were add ...20061828684
biosynthesis of fusarochromanone and its monoacetyl derivative by fusarium equiseti.one fluorescent compound previously named tdp-2 was isolated and purified from a rice culture of fusarium equiseti (alaska 2-2). mass spectral and nuclear magnetic resonance data indicated that it is a c-3'-n-acetyl derivative of fusarochromanone, a newly discovered mycotoxin. time course studies of synthesis of these two compounds on autoclaved rice and czapek-dox medium enriched with soybean peptone indicated that fusarochromanone was converted to tdp-2 in the cultures. a high concentration of ...20092729980
chemical defence in a millipede: evaluation and characterization of antimicrobial activity of the defensive secretion from pachyiulus hungaricus (karsch, 1881) (diplopoda, julida, julidae).the chemical defence of the millipede pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. in total, independently of sex, 44 compounds were identified. all compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. the relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. the two dominant quinones in b ...201627907048
identification and characterization of ethanol utilizing fungal flora of oil refinery contaminated soil.the indigenous fungal flora of three oil refinery contaminated sites (bharuch, valsad and vadodara) of india has been documented in the present investigation. a total seventy-five fungal morphotypes were isolated from these sites and out of them, only fifteen isolates were capable of utilizing ethanol (0-8%; v:v) as a sole source of carbon and energy for growth. ten percent ethanol was completely lethal for the growth of all the isolated fungus. biochemical characterization of the potent ethanol ...201424068535
[identification of filamentous fungi isolated from clinical samples by two different methods and their susceptibility results].molds are widely distributed in nature. aspergillus spp. represent the most frequently observed causative agents, however less frequent pathogens fusarium, scedosporium and zygomycetes have also been considered the most important causes of morbidity and mortality in profoundly immunosuppressed hosts. the aims of this study were to identify filamentous fungi isolated from clinical specimens by conventional and molecular methods, and to detect their antifungal susceptibilities. a total of 6742 cli ...201222399173
isolation and characterisation of an enterocin p-producing enterococcus lactis strain from a fresh shrimp (penaeus vannamei).screening for lactic acid bacteria (lab) from fresh shrimp samples (penaeus vannamei) collected from retail seafood markets in the tunisian's coast, resulted in the isolation of an enterococcus strain termed q1. this strain was selected for its antagonistic activity against pathogenic bacteria such as listeria monocytogenes, pseudomonas aeruginosa, lactococcus garvieae and against fungi (aspergillus niger and fusarium equiseti). the q1 strain was characterised using standard morphological and bi ...201728265787
alkyl-substituted δ-lactones derived from dihydrojasmone and their stereoselective fungi-mediated conversion: production of new antifeedant agents.a chemoenzymatic method was applied to obtain optically pure alkyl-substituted δ-lactones. first, chemical baeyer-villiger oxidation of dihydrojasmone (1) was carried out, affording two new alkyl-substituted δ-lactones: 3,4-dihydro-5-methyl-6-pentyl-2h-pyran-2-one (2) and 5-methyl-6-pentyl-1,13-dioxabicyclo[4.1.0]heptan-2-one (3). in the next step, fungal strains were investigated as biocatalysts to enantioselective conversion of δ-lactones (2) and (3). the fungal cultures: fusarium culmorum am1 ...201627649116
wildly growing asparagus (asparagus officinalis l.) hosts pathogenic fusarium species and accumulates their mycotoxins.asparagus officinalis l. is an important crop in many european countries, likely infected by a number of fusarium species. most of them produce mycotoxins in plant tissues, thus affecting the physiology of the host plant. however, there is lack of information on fusarium communities in wild asparagus, where they would definitely have considerable environmental significance. therefore, the main scientific aim of this study was to identify the fusarium species and quantify their typical mycotoxins ...201626687343
biosynthesis of beauvericin and enniatins in vitro by wheat fusarium species and natural grain contamination in an area of central italy.contamination of wheat grain by beauvericin (bea) and enniatins (ens) is a global emerging mycotoxicological food problem. in this study, strains of fusarium avenaceum (fa), fusarium poae (fp), fusarium equiseti and fusarium sporotrichioides, all potential bea and en producers, isolated from 162 grain samples of durum and soft wheat harvested in 2009 and 2010 collected in an area of central italy, were preliminarily screened for the presence of the esyn1 gene, encoding the multifunctional enzyme ...201525475336
fusarium species isolated from pennisetum clandestinum collected during outbreaks of kikuyu poisoning in cattle in south africa.kikuyu poisoning occurs sporadically in south africa. it is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the eastern cape province of south africa from 2008 to 2010. the kikuyu grass samples were submitted specifically for the isolation and molecular identification of fusarium species, as it was recently suggested ...201425686222
<i>fusarium</i> species isolated from <i>pennisetum clandestinum</i> collected during outbreaks of kikuyu poisoning in cattle in south africa.kikuyu poisoning occurs sporadically in south africa. it is of major economic importance, as valuable dairy cows are often poisoned by it, and once affected, the mortality rate is high. pennisetum clandestinum samples were collected during eight outbreaks of kikuyu poisoning in cattle in the eastern cape province of south africa from 2008 to 2010. the kikuyu grass samples were submitted specifically for the isolation and molecular identification of fusarium species, as it was recently suggested ...201428235286
biotransformation of bicyclic halolactones with a methyl group in the cyclohexane ring into hydroxylactones and their biological activity.the aim of this study was the chemical synthesis of a series of halo- and unsaturated lactones, as well as their microbial transformation products. finally some of their biological activities were assessed. three bicyclic halolactones with a methyl group in the cyclohexane ring were obtained from the corresponding γ,δ-unsaturated ester during a two-step synthesis. these lactones were subjected to screening biotransformation using twenty two fungal strains. these strains were tested on their abil ...201627809258
different culture metabolites of the red sea fungus fusarium equiseti optimize the inhibition of hepatitis c virus ns3/4a protease (hcv pr).the endophytic fungus fusarium equiseti was isolated from the brown alga padina pavonica, collected from the red sea. the fungus was identified by its morphology and 18s rdna. cultivation of this fungal strain in biomalt-peptone medium led to isolation of 12 known metabolites of diketopeprazines and anthraquinones. the organic extract and isolated compounds were screened for their inhibition of hepatitis c virus ns3/4a protease (hcv pr). as a result, the fungal metabolites showed inhibition of h ...201627775589
pathogenic ability and saline stress tolerance of two fusarium isolates from odontesthes bonariensis eggs.several fungal species represent a potential risk to embryos of odontesthes bonariensis (cuvier and valenciennes, 1835), a euryhaline freshwater fish that lives in the pampean inland waters and has potential economic relevance.201626674586
genus-specific primers for study of fusarium communities in field samples.fusarium is a large and diverse genus of fungi of great agricultural and economic importance, containing many plant pathogens and mycotoxin producers. to date, high-throughput sequencing of fusarium communities has been limited by the lack of genus-specific primers targeting regions with high discriminatory power at the species level. in the present study, we evaluated two fusarium-specific primer pairs targeting translation elongation factor 1 (tef1). we also present the new primer pair fa+7/ra ...201526519387
a new subtilase-like protease deriving from fusarium equiseti with high potential for industrial applications.a gene encoding a novel extracellular subtilisin-like protease was cloned from the ascomycete fusarium equiseti and expressed in trichoderma reesei. the f. equiseti protease (fe protease) showed excellent performance in stain removal and good compatibility with several commercial laundry detergent formulations, suggesting that it has high potential for use in various industrial applications. the recombinant enzyme was purified and characterized. the temperature optimum of the fe protease was 60 ...201526178876
identification of different fusarium spp. in allium spp. in germany.in 2013 allium cepa bulbs from different fields in northern and southern germany, seeds and sets from onion breeders were analysed for infestation with fusarium species. the same investigation was done in 2014 with different edible allium spp. from local markets. different fusarium spp. were isolated and identified by morphological characterisation. 24 different fusarium spp. were identified. the diversity of fusarium spp. and the intensity of infestation was higher on edible bulbs compared to t ...201527141742
growth rate and tri5 gene expression profiles of fusarium equiseti strains isolated from spanish cereals cultivated on wheat and barley media at different environmental conditions.fusarium equiseti is a toxigenic species that often contaminates cereal crops from diverse climatic regions such as northern and southern europe. previous results suggested the existence of two distinct populations within this species with differences in toxin profile which largely corresponded to north and south europe (spain). in this work, growth rate profiles of 4 f. equiseti strains isolated from different cereals and distinct spanish regions were determined on wheat and barley based media ...201525500278
fusarochromanone induces g1 cell cycle arrest and apoptosis in cos7 and hek293 cells.fusarochromanone (fc101), a mycotoxin produced by the fungus fusarium equiseti, is frequently observed in the contaminated grains and feedstuffs, which is toxic to animals and humans. however, the underlying molecular mechanism remains to be defined. in this study, we found that fc101 inhibited cell proliferation and induced cell death in cos7 and hek293 cells in a concentration-dependent manner. flow cytometric analysis showed that fc101 induced g1 cell cycle arrest and apoptosis in the cells. ...201425384025
occurrence of root rot and vascular wilt diseases in roselle (hibiscus sabdariffa l.) in upper egypt.roselle (hibiscus sabdariffa l.) family malvaceae is an important crop used in food, cosmetics and pharmaceutics industries. roselle is cultivated mainly in upper egypt (qena and aswan governorates) producing 94% of total production. root rot disease of roselle is one of the most important diseases that attack both seedlings and adult plants causing serious losses in crop productivity and quality. the main objective of the present study is to identify and characterize pathogens associated with r ...201424808737
[distribution of endophytic fungi in oxytropis glabra dc].endophytic fungi were detected and isolated from the stems, leaves, petioles and seeds of oxytropis glabra dc sampled from alashan of inner mongolia to investigate the infection rate and species distribution in different tissues.201323957152
involvement of the salicylic acid signaling pathway in the systemic resistance induced in arabidopsis by plant growth-promoting fungus fusarium equiseti gf19-1.plant growth-promoting fungi (pgpf) are effective biocontrol agents for a number of soil-borne diseases and are known for their ability to trigger induced systemic resistance (isr). in this study, we investigated the mechanisms triggered by pgpf fusarium equiseti gf19-1, which is known to increase pathogen resistance in plants, by using gf19-1 spores and the culture filtrate (cf) to treat the roots of arabidopsis thaliana. subsequently, the leaves were challenged with pseudomonas syringae pv tom ...201323728333
microbial transformation of asiatic acid.asiatic acid (1), a major pentacyclic triterpene of centella asiatica, was subjected to transformation by penicillium lilacinum accc 31890, fusarium equiseti cgmcc 3.3658, and streptomyces griseus cgmcc 4.18 strains. incubation of asiatic acid with p. lilacinum accc 31890 and f. equiseti cgmcc 3.3658 gave an identical product: 2α,3β,15α,23-tetrahydroxyurs-12-en-28-oic acid (2). biotransformation of asiatic acid by s. griseus cgmcc 4.18 resulted in three derivatives: 2α,3β,21β,23-tetrahydroxyurs- ...201323227815
trichothecenes and zearalenone production by fusarium equiseti and fusarium semitectum species isolated from argentinean soybean.fusarium equiseti and fusarium semitectum represent the most abundant species in the fusarium complex isolated from flowers, soybean pods and seeds in argentina. the aim of the present study was to assess the production of major type a and type b trichothecenes (diacetoxyscirpenol, neosolaniol, t-2 toxin and ht-2 toxin, nivalenol, deoxynivalenol) and zearalenone by 40 f. equiseti and 22 f. semitectum isolates on rice culture. mycotoxins were determined by hplc with fluorescence detection after d ...201222830612
phylogenetic analyses and toxigenic profiles of fusarium equiseti and fusarium acuminatum isolated from cereals from southern europe.fusarium equiseti and fusarium acuminatum are toxigenic species that contaminate cereal crops from diverse climatic regions. they are common in spanish cereals. the information available on their phylogenetics and toxigenic profiles is, however, insufficient to assist risk evaluation. in this work, phylogenetic analyses were performed using partial sequences of the translation elongation factor gene (ef-1α) of f. equiseti and f. acuminatum strains isolated from barley and wheat from spain and ot ...201222608228
a novel strain of brevibacillus laterosporus produces chitinases that contribute to its biocontrol potential.a novel strain exhibiting entomopathogenic and chitinolytic activity was isolated from mangrove marsh soil in india. the isolate was identified as brevibacillus laterosporus by phenotypic characterization and 16s rrna sequencing and designated lak1210. when grown in the presence of colloidal chitin as the sole carbon source, the isolate produced extracellular chitinases. chitinase activity was inhibited by allosamidin indicating that the enzymes belong to the family 18 chitinases. the chitinases ...201322543421
polymorphism of mycotoxin biosynthetic genes among fusarium equiseti isolates from italy and poland.fusarium equiseti (corda) saccardo is a soil saprophyte and a weak pathogen, associated with several diseases of fruit and other crops in subtropical and tropical areas, but also in countries with temperate climate. a wide range of secondary metabolites has been identified among natural f. equiseti populations, with zearalenone (zea), fusarochromanone and fusarenon-x being the most common. in present study, the genetic diversity of strains from two populations (from italy and poland) was evaluat ...201222354659
molecular characterization of pathogenic fusarium species in cucurbit plants from kermanshah province, iran.fusarium is one of the important phytopathogenic genera of microfungi causing serious losses on cucurbit plants in kermanshah province, the largest area of cucurbits plantation in iran. therefore, the objectives in this study were to isolate and identify disease-causing fusarium spp. from infected cucurbit plants, to ascertain their pathogenicity, and to determine their phylogenetic relationships. a total of 100 fusarium isolates were obtained from diseased cucurbit plants collected from fields ...201123961146
comparative study of the pathogenicity of seabed isolates of fusarium equiseti and the effect of the composition of the mineral salt medium and temperature on mycelial growth.the pathogenicity of seven strains of fusarium equiseti isolated from seabed soil was evaluated on different host plants showing pre and post emergence damage. radial growth of 27 strains was measured on culture media previously adjusted to different osmotic potentials with either kcl or nacl (-1.50 to -144.54 bars) at 15°, 25° and 35° c. significant differences and interactive effects were observed in the response of mycelia to osmotic potential and temperature.201124031710
biotransformation of the phytoestrogen 8-prenylnaringenin.in order to select microorganisms capable of transforming the potent phytoestrogen 8-prenylnaringenin, preliminary screening tests on 30 fungal cultures were performed. no reports concerning successful biotransformation of 8-prenylnaringenin by this class of organisms have been known so far. fusarium equiseti converted 8-prenylnaringenin into201021138063
fusarium incarnatum/equiseti hemodialysis graft infection.hemodialysis graft infections typically occur as a result of contamination by skin flora at the time of insertion or become secondarily infected after high-grade bacteremia. infection of implanted vascular devices with filamentous fungi is rare. we report a case of infection of an implanted polytetrafluoroethylene dialysis graft with fusarium incarnatum/equiseti that did not grow in cultures of tissue but was identified by molecular means.201024470894
morphological, chemical and molecular differentiation of fusarium equiseti isolated from norwegian cereals.the morphological variation, secondary metabolite profiles and restriction fragment length polymorphisms (rflps) of pcr amplified intergenic spacer (igs) ribosomal dna (rdna) were studied in 27 isolates of fusarium equiseti, 25 isolated from norwegian cereals and 2 from soil obtained from the ibt culture collection (biocentrum, technical university of denmark). all 27 isolates were tested for production of fusarochromanone (fuschr), zearalenone (zea) and the trichothecenes: 15-monoacetoxy-scirpe ...200515734567
[presearch on preventing the medicinal plant diseases with trichoderma harzianum preparation].to control the medicinal plant diseases with the preparation of trichoderma harzianum.200315015261
cytotoxicity of trichothecenes and fusarochromanone produced by fusarium equiseti strains isolated from norwegian cereals.the cytotoxicity and secondary metabolites of 28 norwegian strains of fusarium equiseti have been characterized. trichothecenes and fusarochromanone (fuch) in rice culture extracts of the strains were analysed by gas chromatography-mass spectrometry (gc-ms) and high performance liquid chromatography (hplc). the following metabolites were found in all isolates: fuch, nivalenol (niv), scirpentriol (scirp), 4-acetylnivalenol (4-ac-niv, also called fusarenon-x), 15-acetyl-nivalenol (15-ac-niv), and ...200211913766
directed biosynthesis of new enniatins.new cyclohexadepsipeptides of the enniatin type with potential anthelmintic properties were produced by two different strategies: 1. in vitro synthesis by use of the multienzyme enniatin synthetase, and 2. in vivo precursor feeding of enniatin producing strains fusarium scirpi and fusarium sambucinum. the compounds were analyzed by hplc, various nmr measurements and mass spectrometry. the three n-methyl l-amino acid positions in the enniatin b molecule could be gradually replaced by other (n-met ...200111776434
isolation and immunobiochemical characterization of a major allergen (65 kda) from fusarium equiseti.fusarium equiseti is one of the most important species in the class deuteromycetes (fungi imperfecti). for proper diagnosis and immunotherapy, isolation and characterization of allergens of f. equiseti are necessary. in the present study, culture filtrate (cf) extract of f. equiseti was resolved into 35-37 bands on isoelectric focusing pi (3-9) and sds-page (mol. wt. 10-100 kda). most of them were glycoproteins, as identified by pas staining. f. equiseti cf revealed 15 allergenic proteins on imm ...19989542613
enniatin production by fusarium strains and its effect on potato tuber tissue.several fusarium strains produce the cyclohexadepsipeptide enniatin, a host-nonspecific phytotoxin. enniatins are synthesized by the 347-kda multifunctional enzyme enniatin synthetase. in the present study, 36 fusarium strains derived from a wide range of host plants were characterized with respect to enniatin production in different media. thirteen of these strains produced enniatins on one or more of these media. to determine whether enniatin production affected virulence, an assay on potato t ...199616535227
highly conserved n-methyltransferases as an integral part of peptide synthetases.the methyltransferase portion of the n-methyl-peptide-synthetase gene, synthesizing enniatin from fusarium sambucinum, was amplified with the polymerase chain reaction (pcr) using primers derived from the highly conserved sequences of the flanking peptide synthetase domain. the deduced amino acid sequence of the product shares high similarity to the 430 amino acid methyltransferase portion of enniatin synthetase of fusarium scirpi and the corresponding portions of another fungal peptide syntheta ...19958673002
isolation and structure identification of two new derivatives of the mycotoxin fusarochromenone produced by fusarium equiseti.two new chromone derivatives, 2,2-dimethyl-5-amino-6-(4'-hydroxylbutyryl)- 4-chromone [2] and 2,2-dimethyl-5-amino-6-(2'e-ene-4'-hydroxylbutyryl)- 4-chromone [4], were isolated from rice cultures of fusarium equiseti. their structures were deduced from chemical and spectral data.19957760069
evaluation of some antibiotics on fusarium equiseti causing damping off in guar (cyamopsis tetragonolobe (l.) taub. 20137960930
[the colors produced by fusarium link ex fr. 1821 (deuteromycetes) species in various carbohydrate cultures. i. fusarium equiseti (corda) sacc. 1886].in this study, fusarium equiseti (corda) sacc. 1886 fungus was inoculated in cultures containing glucose, maltose, galactose, mannitol, sucrose, fructose, lactose and starch and control media cultures and were observed at intervals of five days. in most of the cultures various tones of brown were observed. our results showed a correlation when compared with literature in general no different results were observed. three color catalogs were used for the identification of these colors.19938421446
enniatin synthetases from different fusaria exhibiting distinct amino acid specificities.enniatin synthetases from the cyclodepsipeptide producers fusarium lateritium and fusarium sambucinum were purified to homogeneity and characterized. like the previously described enniatin synthetase from fusarium scirpi both enzymes consist of a single polypeptide chain and are very similar concerning their mr (250 kdaltons) and reaction mechanism. limited proteolytic digests show only slight differences in their patterns in sds-gels. interestingly the synthetases differ in their amino acids sp ...19921399848
mass spectral analysis and fragment ion structure of fusarochromanone.fusarochromanone is a mycotoxin produced by fusarium equiseti that is implicated in the poultry disease tibial dyschrondroplasia. electron impact ionization tandem mass spectrometry was used to elucidate probable structures of fragment ions found at m/z 274, 275, 261, 233, 218 and 191 and for devising an analytical rationale for the metabolites of the parent compound. in addition, a sensitive, qualitative liquid chromatographic technique using direct injection continuous-flow fast atom bombardme ...19911799587
immunochromatography of fusarochromanone mycotoxins.the present paper describes an enzyme-linked immunoassay (elisa) used in combination with thin-layer chromatography (tlc) and liquid chromatography (lc) for determination of fusarochromanone (tdp) mycotoxins in barley, wheat, and a fusarium culture grown in rice and corn. the mycotoxins were first extracted from the sample with 100% methanol and subjected to tlc or lc without additional cleanup treatment. individual fractions eluted from tlc or lc were acetylated, then analyzed by elisa. determi ...20151917812
[population of fusarium spp. in soils from the arid regions and effect of water potential on their growth].in trials of wheat establishment in irrigated areas in the patagonian valleys (picún leufú), sectors with plants showing symptoms of typical foot-rot were detected. in order to investigate the phytopathogenic mycoflora of these soils (arid zone), the population of fusarium spp was analysed quali- and quantitatively and its tolerance to water stress by measuring the mycelial growth in the presence of different water potentials. fusarium spp detected in that type of soil did not coincide with thos ...20131815274
fusarochromanone production by fusarium isolates.sixty two fusarium isolates representing nine species from many parts of the world were screened for fusarochromanone production. a simplified method for the detection of fusarochromanone in culture filtrates or grain cultures was used. under uv irradiation (364 nm) the chloroform phase from fusarochromanone-positive culture extracts fluoresced a characteristic bright blue color. results were confirmed by thin-layer-chromatography comparison with pure fusarochromanone standards. detection was po ...19902285312
isolation and structural identification of a new metabolite of fusarium equiseti.a fluorescent compound was isolated and purified from rice cultures of fusarium equiseti (alaska 2-2). mass spectrometry and nuclear magnetic resonance data indicated that its structure is 2,2-dimethyl-5-amino-6-(3'-hydroxyl-4'-methoxyl-butyryl)-4-chromone. it is an analog of the mycotoxin fusarochromanone, in which the amino group on c-3' is replaced by a hydroxyl group and the hydroxyl group on c-4' is replaced by a methoxyl group.199016348305
constitutive expression of enniatin synthetase during fermentative growth of fusarium scirpi.the production of enniatins by fusarium scirpi during fermentative growth in submerged cultures was measured. the fungus produced the antibiotic during mycelial growth, but not during the stationary phase of cultivation. by contrast, enniatin synthetase, the enzyme responsible for enniatin synthesis, was present during growth, during the stationary phase, and even in spores. similarly, the enniatin synthetase mrna was present at every stage of the cultivation of the fungus. therefore, this multi ...198816347758
adsorption of lithocholic acid to fusarium equiseti m41 as an essential process in its conversion to ursodeoxycholic acid.fusarium equiseti m41 converts lithocholic acid to ursodeoxycholic acid. adsorption of lithocholic acid particles to mycelia of f. equiseti m41 is essential in the conversion of lithocholic acid to ursodeoxycholic acid. production of ursodeoxycholic acid was negligible when particles of lithocholic acid were absent. as the concentration of lithocholic acid particles increased, both the amount of mycelium-bound lithocholic acid and the production of ursodeoxycholic acid increased hyperbolically ( ...198816347578
optimum conditions for ursodeoxycholic acid production from lithocholic acid by fusarium equiseti m41.ursodeoxycholic acid dissolves cholesterol gallstones in humans. in the present study optimum conditions for ursodeoxycholic acid production by fusarium equiseti m41 were studied. resting mycelia of f. equiseti m41 showed maximum conversion at 28 degrees c, ph 8.0, and dissolved oxygen tension of higher than 60% saturation. monovalent cations, such as na+, k+, and rb+, stimulated the conversion rate more than twofold. in the presence of 0.5 m kcl, the initial uptake rate and equilibrium concentr ...19853985610
microbial production of ursodeoxycholic acid from lithocholic acid by fusarium equiseti m41.a fungus identified as fusarium equiseti was isolated from soil and found to carry out 7beta-hydroxylation of lithocholic acid to ursodeoxycholic acid (35% yield; 350 mg/liter) in 112 h.198216346142
equisetin, an antibiotic from fusarium equiseti nrrl 5537, identified as a derivative of n-methyl-2,4-pyrollidone. 1979541270
phytotoxic compounds produced by fusarium equiseti. vi. 4-beta-8-alpha,15-triacetoxy-12,13-epoxytrichothec-9-ene-3-alpha,7-alpha-diol. 19705460867
phytotoxic compounds produced by fusarium equiseti. v. transformation products of 4-beta,15-diacetoxy-3-alpha,7-alpha-dihydroxy-12,13-epoxytrichothec-9-en-8-one and the structures of nivalenol and fusarenone. 19705460866
Displaying items 1 - 100 of 104